AK1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	AK1
Entrez Gene ID	280715
Full Name	adenylate kinase 1
General protein information	Preferred Names adenylate kinase 1 Names adenylate kinase isoenzyme 1 AK 1 ATP-AMP transphosphorylase 1 ATP:AMP phosphotransferase adenylate kinase 1, soluble adenylate monophosphate kinase myokinase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015473595.1	XP_015329081.1	adenylate kinase isoenzyme 1 isoform X1
XM_005213172.1	XP_005213229.1	adenylate kinase isoenzyme 1 isoform X2
XM_024998258.1	XP_024854026.1	adenylate kinase isoenzyme 1 isoform X2
XM_024998257.1	XP_024854025.1	adenylate kinase isoenzyme 1 isoform X2
XM_005213172.2	XP_005213229.1	adenylate kinase isoenzyme 1 isoform X2
XM_015473595.2	XP_015329081.2	adenylate kinase isoenzyme 1 isoform X1
NM_001013582.1	NP_001013600.1	adenylate kinase isoenzyme 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AK1	NP_001124519.1
Bos taurus (cattle)	AK1	NP_001013600.1
Arabidopsis thaliana (thale cress)	AT4G25280	NP_194258.2
Homo sapiens (human)	AK1	NP_000467.1
	AK1	XP_002800218.1
Gallus gallus (chicken)	AK1	NP_990440.1
Mus musculus (house mouse)	Ak1	NP_067490.1
Rattus norvegicus (Norway rat)	Ak1	NP_077325.2
Xenopus tropicalis (tropical clawed frog)	ak1	NP_001006817.1
Pan troglodytes (chimpanzee)	AK1	XP_003951582.1
Danio rerio (zebrafish)	ak1	NP_001003993.1
Drosophila melanogaster (fruit fly)	Adk1	NP_729792.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009317	XP_309986.3
Caenorhabditis elegans (roundworm)	F38B2.4	NP_001257141.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Seminal plasma proteins regulate the association of lipids and proteins within detergent-resistant membrane domains of bovine spermatozoa.
Girouard J, Frenette G, Sullivan R
Biology of reproduction78(5)921-31(2008 May)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

Studies on adenosine triphosphate transphosphorylases. Amino acid sequence of rabbit muscle ATP-AMP transphosphorylase.
Kuby SA, Palmieri RH, Frischat A, Fischer AH, Wu LH, Maland L, Manship M
Biochemistry23(11)2393-9(1984 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The sperm-associated proteins, P25b and adenylate kinase 1, are linked to detergent-resistant membrane of epididymal spermatozoa, but were exclusively associated with detergent-soluble material in ejaculated spermatozoa.
Title: Seminal plasma proteins regulate the association of lipids and proteins within detergent-resistant membrane domains of bovine spermatozoa.

The following AK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa24321	NM_001013582.1 Latest version!	Bos taurus adenylate kinase 1 (AK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa24321	XM_024998258.1 Latest version!	Bos taurus adenylate kinase 1 (AK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa24321	XM_005213172.2 Latest version!	Bos taurus adenylate kinase 1 (AK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa24321	XM_024998257.1 Latest version!	Bos taurus adenylate kinase 1 (AK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa220087	XM_015473595.2 Latest version!	Bos taurus adenylate kinase 1 (AK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa91967	XM_015473595.1 Latest version!	Bos taurus adenylate kinase 1 (AK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OBa24321	XM_005213172.1	Bos taurus adenylate kinase 1 (AK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AK1 ( NM_001013582.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001013600.1 Bos taurus adenylate kinase 1 (AK1), mRNA.

Price & Availability↑

CloneID	OBa24321
Clone ID Related Accession (Same CDS sequence)	XM_005213172.1 , XM_024998258.1 , XM_024998257.1 , XM_005213172.2 , NM_001013582.1
Accession Version	NM_001013582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	adenylate kinase isoenzyme 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT020951.1. ##Evidence-Data-START## Transcript exon combination :: BT020951.1, BC114796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145450, SAMN03145467 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013600.1
CDS	76..660
Translation	MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSARGKMLSEIMEKGQLVPLETVLDMLRDAMVAKVDTSKGFLIDGYPREVQQGEEFERRIAQPTLLLYVDAGPETMTKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDNVFSQVCTHLDALK

Target ORF information:

RefSeq Version	NM_001013582.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate kinase 1 (AK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013582.1

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AK1 ( XM_005213172.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213229.1 Bos taurus adenylate kinase 1 (AK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa24321
Clone ID Related Accession (Same CDS sequence)	XM_005213172.1 , XM_024998258.1 , XM_024998257.1 , XM_005213172.2 , NM_001013582.1
Accession Version	XM_005213172.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate kinase isoenzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213229.1
CDS	337..921
Translation	MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSARGKMLSEIMEKGQLVPLETVLDMLRDAMVAKVDTSKGFLIDGYPREVQQGEEFERRIAQPTLLLYVDAGPETMTKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDNVFSQVCTHLDALK

Target ORF information:

RefSeq Version	XM_005213172.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate kinase 1 (AK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213172.1

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AK1 ( XM_024998258.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854026.1 Bos taurus adenylate kinase 1 (AK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa24321
Clone ID Related Accession (Same CDS sequence)	XM_005213172.1 , XM_024998258.1 , XM_024998257.1 , XM_005213172.2 , NM_001013582.1
Accession Version	XM_024998258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate kinase isoenzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854026.1
CDS	164..748
Translation	MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSARGKMLSEIMEKGQLVPLETVLDMLRDAMVAKVDTSKGFLIDGYPREVQQGEEFERRIAQPTLLLYVDAGPETMTKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDNVFSQVCTHLDALK

Target ORF information:

RefSeq Version	XM_024998258.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate kinase 1 (AK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998258.1

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AK1 ( XM_024998257.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854025.1 Bos taurus adenylate kinase 1 (AK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa24321
Clone ID Related Accession (Same CDS sequence)	XM_005213172.1 , XM_024998258.1 , XM_024998257.1 , XM_005213172.2 , NM_001013582.1
Accession Version	XM_024998257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate kinase isoenzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854025.1
CDS	78..662
Translation	MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSARGKMLSEIMEKGQLVPLETVLDMLRDAMVAKVDTSKGFLIDGYPREVQQGEEFERRIAQPTLLLYVDAGPETMTKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDNVFSQVCTHLDALK

Target ORF information:

RefSeq Version	XM_024998257.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate kinase 1 (AK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998257.1

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AK1 ( XM_005213172.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213229.1 Bos taurus adenylate kinase 1 (AK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa24321
Clone ID Related Accession (Same CDS sequence)	XM_005213172.1 , XM_024998258.1 , XM_024998257.1 , XM_005213172.2 , NM_001013582.1
Accession Version	XM_005213172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate kinase isoenzyme 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213229.1
CDS	368..952
Translation	MEEKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSARGKMLSEIMEKGQLVPLETVLDMLRDAMVAKVDTSKGFLIDGYPREVQQGEEFERRIAQPTLLLYVDAGPETMTKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDNVFSQVCTHLDALK

Target ORF information:

RefSeq Version	XM_005213172.2
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate kinase 1 (AK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213172.2

ORF Insert Sequence:

ATGGAAGAGA AGCTGAAGAA AACCAAGATC ATCTTTGTGG TGGGCGGGCC CGGCTCGGGG 
AAGGGCACCC AGTGTGAGAA GATTGTGCAG AAGTACGGCT ACACCCACCT CTCCACCGGA 
GACCTCCTGC GGGCCGAGGT CAGCTCGGGC TCGGCTAGGG GCAAGATGCT GTCAGAAATC 
ATGGAGAAGG GGCAGCTGGT GCCACTGGAG ACAGTGCTGG ACATGCTCCG AGACGCCATG 
GTGGCCAAGG TAGATACTTC CAAAGGCTTT CTGATCGACG GCTACCCCCG GGAGGTGCAG 
CAGGGGGAGG AGTTTGAGCG GAGGATCGCA CAGCCCACAC TACTGCTGTA TGTGGACGCC 
GGCCCTGAGA CCATGACCAA GCGGCTCCTG AAGCGCGGAG AGACCAGCGG ACGTGTGGAC 
GACAACGAAG AGACCATCAA GAAGCGTCTG GAAACCTACT ACAAGGCCAC AGAGCCCGTC 
ATCGCCTTCT ACGAGAAACG CGGCATTGTT CGCAAGGTCA ACGCTGAAGG CTCTGTGGAC 
AATGTCTTCT CCCAGGTCTG CACCCACCTG GACGCCCTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AK1 ( XM_015473595.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329081.2 Bos taurus adenylate kinase 1 (AK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220087
Clone ID Related Accession (Same CDS sequence)	XM_015473595.2
Accession Version	XM_015473595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	adenylate kinase isoenzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGGT TGCCAACTCC ACACTGGTAC TGCCTAGGGG AGGCAGGCGA TGGGCAGGCC 
CAGAGCCTGA GGGGGGAAGG CTGGGGGACA CAGCAGACCG GGACCATGGG GGCCTGCTGC 
TCGGGGACAG ACCCCCGCAT CATGGAAGAC TCTAGAGCCA GAGAGAAGCT GAAGAAAACC 
AAGATCATCT TTGTGGTGGG CGGGCCCGGC TCGGGGAAGG GCACCCAGTG TGAGAAGATT 
GTGCAGAAGT ACGGCTACAC CCACCTCTCC ACCGGAGACC TCCTGCGGGC CGAGGTCAGC 
TCGGGCTCGG CTAGGGGCAA GATGCTGTCA GAAATCATGG AGAAGGGGCA GCTGGTGCCA 
CTGGAGACAG TGCTGGACAT GCTCCGAGAC GCCATGGTGG CCAAGGTAGA TACTTCCAAA 
GGCTTTCTGA TCGACGGCTA CCCCCGGGAG GTGCAGCAGG GGGAGGAGTT TGAGCGGAGG 
ATCGCACAGC CCACACTACT GCTGTATGTG GACGCCGGCC CTGAGACCAT GACCAAGCGG 
CTCCTGAAGC GCGGAGAGAC CAGCGGACGT GTGGACGACA ACGAAGAGAC CATCAAGAAG 
CGTCTGGAAA CCTACTACAA GGCCACAGAG CCCGTCATCG CCTTCTACGA GAAACGCGGC 
ATTGTTCGCA AGGTCAACGC TGAAGGCTCT GTGGACAATG TCTTCTCCCA GGTCTGCACC 
CACCTGGACG CCCTCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329081.2
CDS	1..741
Translation	MLWLPTPHWYCLGEAGDGQAQSLRGEGWGTQQTGTMGACCSGTDPRIMEDSRAREKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSARGKMLSEIMEKGQLVPLETVLDMLRDAMVAKVDTSKGFLIDGYPREVQQGEEFERRIAQPTLLLYVDAGPETMTKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDNVFSQVCTHLDALK

Target ORF information:

RefSeq Version	XM_015473595.2
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate kinase 1 (AK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473595.2

ORF Insert Sequence:

ATGTTGTGGT TGCCAACTCC ACACTGGTAC TGCCTAGGGG AGGCAGGCGA TGGGCAGGCC 
CAGAGCCTGA GGGGGGAAGG CTGGGGGACA CAGCAGACCG GGACCATGGG GGCCTGCTGC 
TCGGGGACAG ACCCCCGCAT CATGGAAGAC TCTAGAGCCA GAGAGAAGCT GAAGAAAACC 
AAGATCATCT TTGTGGTGGG CGGGCCCGGC TCGGGGAAGG GCACCCAGTG TGAGAAGATT 
GTGCAGAAGT ACGGCTACAC CCACCTCTCC ACCGGAGACC TCCTGCGGGC CGAGGTCAGC 
TCGGGCTCGG CTAGGGGCAA GATGCTGTCA GAAATCATGG AGAAGGGGCA GCTGGTGCCA 
CTGGAGACAG TGCTGGACAT GCTCCGAGAC GCCATGGTGG CCAAGGTAGA TACTTCCAAA 
GGCTTTCTGA TCGACGGCTA CCCCCGGGAG GTGCAGCAGG GGGAGGAGTT TGAGCGGAGG 
ATCGCACAGC CCACACTACT GCTGTATGTG GACGCCGGCC CTGAGACCAT GACCAAGCGG 
CTCCTGAAGC GCGGAGAGAC CAGCGGACGT GTGGACGACA ACGAAGAGAC CATCAAGAAG 
CGTCTGGAAA CCTACTACAA GGCCACAGAG CCCGTCATCG CCTTCTACGA GAAACGCGGC 
ATTGTTCGCA AGGTCAACGC TGAAGGCTCT GTGGACAATG TCTTCTCCCA GGTCTGCACC 
CACCTGGACG CCCTCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AK1 ( XM_015473595.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329081.1 Bos taurus adenylate kinase 1 (AK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa91967
Clone ID Related Accession (Same CDS sequence)	XM_015473595.1
Accession Version	XM_015473595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	adenylate kinase isoenzyme 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCT GCTGCTCGGG GACAGACCCC CGCATCATGG AAGACTCTAG AGCCAGAGAG 
AAGCTGAAGA AAACCAAGAT CATCTTTGTG GTGGGCGGGC CCGGCTCGGG GAAGGGCACC 
CAGTGTGAGA AGATTGTGCA GAAGTACGGC TACACCCACC TCTCCACCGG AGACCTCCTG 
CGGGCCGAGG TCAGCTCGGG CTCGGCTAGG GGCAAGATGC TGTCAGAAAT CATGGAGAAG 
GGGCAGCTGG TGCCACTGGA GACAGTGCTG GACATGCTCC GAGACGCCAT GGTGGCCAAG 
GTAGATACTT CCAAAGGCTT TCTGATCGAC GGCTACCCCC GGGAGGTGCA GCAGGGGGAG 
GAGTTTGAGC GGAGGATCGC ACAGCCCACA CTACTGCTGT ATGTGGACGC CGGCCCTGAG 
ACCATGACCA AGCGGCTCCT GAAGCGCGGA GAGACCAGCG GACGTGTGGA CGACAACGAA 
GAGACCATCA AGAAGCGTCT GGAAACCTAC TACAAGGCCA CAGAGCCCGT CATCGCCTTC 
TACGAGAAAC GCGGCATTGT TCGCAAGGTC AACGCTGAAG GCTCTGTGGA CAATGTCTTC 
TCCCAGGTCT GCACCCACCT GGACGCCCTC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329081.1
CDS	16..651
Translation	MGACCSGTDPRIMEDSRAREKLKKTKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSARGKMLSEIMEKGQLVPLETVLDMLRDAMVAKVDTSKGFLIDGYPREVQQGEEFERRIAQPTLLLYVDAGPETMTKRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDNVFSQVCTHLDALK

Target ORF information:

RefSeq Version	XM_015473595.1
Organism	Bos taurus(cattle)
Definition	Bos taurus adenylate kinase 1 (AK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473595.1

ORF Insert Sequence:

ATGGGGGCCT GCTGCTCGGG GACAGACCCC CGCATCATGG AAGACTCTAG AGCCAGAGAG 
AAGCTGAAGA AAACCAAGAT CATCTTTGTG GTGGGCGGGC CCGGCTCGGG GAAGGGCACC 
CAGTGTGAGA AGATTGTGCA GAAGTACGGC TACACCCACC TCTCCACCGG AGACCTCCTG 
CGGGCCGAGG TCAGCTCGGG CTCGGCTAGG GGCAAGATGC TGTCAGAAAT CATGGAGAAG 
GGGCAGCTGG TGCCACTGGA GACAGTGCTG GACATGCTCC GAGACGCCAT GGTGGCCAAG 
GTAGATACTT CCAAAGGCTT TCTGATCGAC GGCTACCCCC GGGAGGTGCA GCAGGGGGAG 
GAGTTTGAGC GGAGGATCGC ACAGCCCACA CTACTGCTGT ATGTGGACGC CGGCCCTGAG 
ACCATGACCA AGCGGCTCCT GAAGCGCGGA GAGACCAGCG GACGTGTGGA CGACAACGAA 
GAGACCATCA AGAAGCGTCT GGAAACCTAC TACAAGGCCA CAGAGCCCGT CATCGCCTTC 
TACGAGAAAC GCGGCATTGT TCGCAAGGTC AACGCTGAAG GCTCTGTGGA CAATGTCTTC 
TCCCAGGTCT GCACCCACCT GGACGCCCTC AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Seminal plasma proteins regulate the association of lipids and proteins within detergent-resistant membrane domains of bovine spermatozoa.
Biology of reproduction78(5)921-31(2008 May)
Girouard J,Frenette G,Sullivan R

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

Studies on adenosine triphosphate transphosphorylases. Amino acid sequence of rabbit muscle ATP-AMP transphosphorylase.
Biochemistry23(11)2393-9(1984 May)
Kuby SA,Palmieri RH,Frischat A,Fischer AH,Wu LH,Maland L,Manship M

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AK1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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