TH cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TH
Entrez Gene ID	280707
Full Name	tyrosine hydroxylase
General protein information	Preferred Names tyrosine hydroxylase Names tyrosine 3-monooxygenase tyrosine 3-hydroxylase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024986968.1	XP_024842736.1	tyrosine 3-monooxygenase isoform X1
XM_015461329.1	XP_015316815.1	tyrosine 3-monooxygenase isoform X1
NM_173884.2	NP_776309.1	tyrosine 3-monooxygenase
XM_015461329.2	XP_015316815.1	tyrosine 3-monooxygenase isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TH	NP_954987.2
Mus musculus (house mouse)	Th	NP_033403.1
Drosophila melanogaster (fruit fly)	ple	NP_476897.1
Bos taurus (cattle)	TH	NP_776309.1
Rattus norvegicus (Norway rat)	Th	NP_036872.1
	TH	XP_002799487.1
Canis lupus familiaris (dog)	TH	NP_001002966.1
Pan troglodytes (chimpanzee)	TH	XP_003312902.2
Gallus gallus (chicken)	TH	NP_990136.1
Danio rerio (zebrafish)	th	NP_571224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006023	XP_316061.3
Xenopus tropicalis (tropical clawed frog)	LOC100488900	XP_002938944.2
Caenorhabditis elegans (roundworm)	cat-2	NP_001254009.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of tyrosine hydroxylase is preserved across different homo- and heterodimeric 14-3-3 proteins.
Ghorbani S, Fossbakk A, Jorge-Finnigan A, Flydal MI, Haavik J, Kleppe R
Amino acids48(5)1221-9(2016 May)

Interferon-alpha signalling in bovine adrenal chromaffin cells: involvement of signal-transducer and activator of transcription 1 and 2, extracellular signal-regulated protein kinases 1/2 and serine 31 phosphorylation of tyrosine hydroxylase.
Douglas SA, Bunn SJ
Journal of neuroendocrinology21(3)200-7(2009 Mar)

Retinol activates tyrosine hydroxylase acutely by increasing the phosphorylation of serine40 and then serine31 in bovine adrenal chromaffin cells.
Gelain DP, Moreira JC, Bevilaqua LR, Dickson PW, Dunkley PR
Journal of neurochemistry103(6)2369-79(2007 Dec)

PACAP stimulates the sustained phosphorylation of tyrosine hydroxylase at serine 40.
Bobrovskaya L, Gelain DP, Gilligan C, Dickson PW, Dunkley PR
Cellular signalling19(6)1141-9(2007 Jun)

Predicted amino acid sequence of bovine tyrosine hydroxylase and its similarity to tyrosine hydroxylases from other species.
Saadat S, Stehle AD, Lamouroux A, Mallet J, Thoenen H
Journal of neurochemistry51(2)572-8(1988 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TH is a robust interaction partner of different 14-3-3 dimer types with moderate variability between the 14-3-3 dimers on their regulation of TH.
Title: Regulation of tyrosine hydroxylase is preserved across different homo- and heterodimeric 14-3-3 proteins.

IFN-alpha mediated activation of ERK1/2 appeared to be responsible for the increased phosphorylation of tyrosine hydroxylase.
Title: Interferon-alpha signalling in bovine adrenal chromaffin cells: involvement of signal-transducer and activator of transcription 1 and 2, extracellular signal-regulated protein kinases 1/2 and serine 31 phosphorylation of tyrosine hydroxylase.

Retinol activates tyrosine hydroxylase via two sequential non-genomic mechanisms, which have not previously been characterized. These mechanisms are likely to operate in vivo to facilitate the stress response.
Title: Retinol activates tyrosine hydroxylase acutely by increasing the phosphorylation of serine40 and then serine31 in bovine adrenal chromaffin cells.

The sustained activation of TH that occurred as a result of TH phosphorylation at Ser40 could maintain the synthesis of catecholamines without the need for further stimulus of the adrenal cells or increased TH protein synthesis.
Title: PACAP stimulates the sustained phosphorylation of tyrosine hydroxylase at serine 40.

The following TH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41636	NM_173884.2 Latest version!	Bos taurus tyrosine hydroxylase (TH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa231827	XM_024986968.1 Latest version!	Bos taurus tyrosine hydroxylase (TH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa231828	XM_015461329.2 Latest version!	Bos taurus tyrosine hydroxylase (TH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa89144	XM_015461329.1 Latest version!	Bos taurus tyrosine hydroxylase (TH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TH ( NM_173884.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776309.1 Bos taurus tyrosine hydroxylase (TH), mRNA.

Price & Availability↑

CloneID	OBa41636
Clone ID Related Accession (Same CDS sequence)	NM_173884.2
Accession Version	NM_173884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Bos taurus(cattle)
Product	tyrosine 3-monooxygenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M36705.1. On or before Oct 4, 2005 this sequence version replaced XM_865665.1, NM_173884.1. ##Evidence-Data-START## Transcript exon combination :: M36705.1, M36794.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGCCTACCC CCAACGCCGC CTCGCCGCAG GCCAAGGGCT TCCGCAGGGC CGTCTCCGAG 
CTGGACGCCA AGCAGGCTGA GGCCATCATG TCCCCACGCT TCGTCGGGAG GCGGCAGAGC 
CTCATCCAGG ACGCACGCAA GGAGCGGGAG AAGGCGGAGG CCGCGGCCTC ATCCTCGGAG 
TCCGCGGAGG CGGGCAGCCT GGTGGAGAGG GACGGGAAGG CGGTGCTGAC CCTGCTGTTC 
GCCCTGCGGC CCACCAAGCC CCCTGCGCTG ACCCGGGCTA TCAAGGTGTT TGAGACATTC 
GAAGCCCACC TCCACCACCT GGAGACCCGA CCGGCCCAGC CACTGCGGGC GGGGAGCCCG 
CCCCTGGAGT GCTTCGTGCG CTGTGAGGTG CCCGGCCCAG TGGTGCCCGC CCTGCTGAGC 
GCCCTGCGCC GCGTGGCAGA AGATGTGCGT GCCGCCGGGG AGAGCAAAGT CCTCTGGTTC 
CCAAGGAAAG TGTCCGAGCT GGACAAGTGT CACCACCTCG TCACCAAGTT TGACCCTGAC 
CTGGACTTGG ATCATCCCGG CTTCTCCGAC CAGGCGTACC GCCAGCGCAG GAAGCTGATC 
GCCGAGATCG CCTTCCAGTA CAAGCAAGGC GACCCCATTC CCCACGTGGA GTACACAGCC 
GAGGAGACAG CCACCTGGAA GGAGGTCTAC TCCACGCTGC GGGGCCTGTA CCCCACCCAC 
GCCTGCCGCG AGCACCTGGA GGCCTTCGAG CTGCTGGAGC GCTTCTGCGG GTACCGCGAG 
GACCGAATCC CGCAGCTGGA GGACGTCTCA CGCTTCCTGA AGGAGAGGAC CGGCTTCCAG 
CTGCGGCCCG TGGCCGGCCT GCTGTCCGCG CGGGACTTCC TGGCCAGCCT GGCCTTCCGC 
GTGTTCCAGT GCACCCAGTA CATCCGCCAC GCCTCCTCGC CCATGCACTC CCCGGAGCCG 
GACTGCTGCC ACGAGCTGCT GGCACATGGC CCAATGCTCG CCGACCGGAC CTTCGCGCAG 
TTTTCCCAGG ACATCGGGCT CGCATCCTTG GGAGTGTCGG ATGAGGAAAT TGAGAAGCTG 
TCCACGCTGT ACTGGTTCAC CGTGGAGTTC GGGCTATGCA AACAGAATGG AGAGGTGAAC 
GCGTACGGCG CGGGGCTGCT GTCCTCCTAC GGGGAGCTCC TGCACTCCCT TTCCGAGGAG 
CCCGAGATCC GGGCCTTCGA CCCTGATGCA GCGGCCGTGC AGCCCTATCA GGACCAGACC 
TACCAGCCCG TCTACTTCGT GTCTGAGAGC TTCAGCGATG CCAAGGACAA GCTCAGGAGC 
TACGCCTCCC GCATCCAGCG CCCCTTCTCT GTGAAGTTTG ACCCGTACAC ACTGGCCATC 
GACGTGCTGG ACAGTCCCCA CGCCATCCGG CGCGCCCTGG ACGGCGTCCA GGACGAGATG 
CAGGCCCTGG CCCATGCTCT GAACGCCATC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776309.1
CDS	19..1494
Translation	MPTPNAASPQAKGFRRAVSELDAKQAEAIMSPRFVGRRQSLIQDARKEREKAEAAASSSESAEAGSLVERDGKAVLTLLFALRPTKPPALTRAIKVFETFEAHLHHLETRPAQPLRAGSPPLECFVRCEVPGPVVPALLSALRRVAEDVRAAGESKVLWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQAYRQRRKLIAEIAFQYKQGDPIPHVEYTAEETATWKEVYSTLRGLYPTHACREHLEAFELLERFCGYREDRIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLAHGPMLADRTFAQFSQDIGLASLGVSDEEIEKLSTLYWFTVEFGLCKQNGEVNAYGAGLLSSYGELLHSLSEEPEIRAFDPDAAAVQPYQDQTYQPVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPHAIRRALDGVQDEMQALAHALNAIS

Target ORF information:

RefSeq Version	NM_173884.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine hydroxylase (TH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173884.2

ORF Insert Sequence:

ATGCCTACCC CCAACGCCGC CTCGCCGCAG GCCAAGGGCT TCCGCAGGGC CGTCTCCGAG 
CTGGACGCCA AGCAGGCTGA GGCCATCATG TCCCCACGCT TCGTCGGGAG GCGGCAGAGC 
CTCATCCAGG ACGCACGCAA GGAGCGGGAG AAGGCGGAGG CCGCGGCCTC ATCCTCGGAG 
TCCGCGGAGG CGGGCAGCCT GGTGGAGAGG GACGGGAAGG CGGTGCTGAC CCTGCTGTTC 
GCCCTGCGGC CCACCAAGCC CCCTGCGCTG ACCCGGGCTA TCAAGGTGTT TGAGACATTC 
GAAGCCCACC TCCACCACCT GGAGACCCGA CCGGCCCAGC CACTGCGGGC GGGGAGCCCG 
CCCCTGGAGT GCTTCGTGCG CTGTGAGGTG CCCGGCCCAG TGGTGCCCGC CCTGCTGAGC 
GCCCTGCGCC GCGTGGCAGA AGATGTGCGT GCCGCCGGGG AGAGCAAAGT CCTCTGGTTC 
CCAAGGAAAG TGTCCGAGCT GGACAAGTGT CACCACCTCG TCACCAAGTT TGACCCTGAC 
CTGGACTTGG ATCATCCCGG CTTCTCCGAC CAGGCGTACC GCCAGCGCAG GAAGCTGATC 
GCCGAGATCG CCTTCCAGTA CAAGCAAGGC GACCCCATTC CCCACGTGGA GTACACAGCC 
GAGGAGACAG CCACCTGGAA GGAGGTCTAC TCCACGCTGC GGGGCCTGTA CCCCACCCAC 
GCCTGCCGCG AGCACCTGGA GGCCTTCGAG CTGCTGGAGC GCTTCTGCGG GTACCGCGAG 
GACCGAATCC CGCAGCTGGA GGACGTCTCA CGCTTCCTGA AGGAGAGGAC CGGCTTCCAG 
CTGCGGCCCG TGGCCGGCCT GCTGTCCGCG CGGGACTTCC TGGCCAGCCT GGCCTTCCGC 
GTGTTCCAGT GCACCCAGTA CATCCGCCAC GCCTCCTCGC CCATGCACTC CCCGGAGCCG 
GACTGCTGCC ACGAGCTGCT GGCACATGGC CCAATGCTCG CCGACCGGAC CTTCGCGCAG 
TTTTCCCAGG ACATCGGGCT CGCATCCTTG GGAGTGTCGG ATGAGGAAAT TGAGAAGCTG 
TCCACGCTGT ACTGGTTCAC CGTGGAGTTC GGGCTATGCA AACAGAATGG AGAGGTGAAC 
GCGTACGGCG CGGGGCTGCT GTCCTCCTAC GGGGAGCTCC TGCACTCCCT TTCCGAGGAG 
CCCGAGATCC GGGCCTTCGA CCCTGATGCA GCGGCCGTGC AGCCCTATCA GGACCAGACC 
TACCAGCCCG TCTACTTCGT GTCTGAGAGC TTCAGCGATG CCAAGGACAA GCTCAGGAGC 
TACGCCTCCC GCATCCAGCG CCCCTTCTCT GTGAAGTTTG ACCCGTACAC ACTGGCCATC 
GACGTGCTGG ACAGTCCCCA CGCCATCCGG CGCGCCCTGG ACGGCGTCCA GGACGAGATG 
CAGGCCCTGG CCCATGCTCT GAACGCCATC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TH ( XM_024986968.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842736.1 Bos taurus tyrosine hydroxylase (TH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231827
Clone ID Related Accession (Same CDS sequence)	XM_024986968.1
Accession Version	XM_024986968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tyrosine 3-monooxygenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACCC CCAACGCCGC CTCGCCGCAG GCCAAGGGCT TCCGCAGGGC CGTCTCCGAG 
CTGGACGCCA AGCAGGCTGA GGCCATCATG TCCCCACGCT TCGTCGGGAG GCGGCAGAGC 
CTCATCCAGG ACGCACGCAA GGAGCGGGAG AAGGCGGAGG CCGCGGCCTC ATCCTCGGAG 
TCCGCGGAGG CAGGCAGCCT GGTGGAGAGG GACGGGAAGG CGGTGCTGAC CCTGCTGTTC 
GCCCTGCGGC CCACCAAGCC CCCTGCGCTG ACCCGGGCTA TCAAGGTGTT TGAGACATTC 
GAAGCCCACC TCCACCACCT GGAGACCCGA CCGGCCCAGC CACTGCGGGC GGGGAGCCCG 
CCCCTGGAGT GCTTCGTGCG CTGTGAGGTG CCCGGCCCAG TGGTGCCCGC CCTGCTGAGC 
GCCCTGCGCC GCGTGGCAGA AGATGTGCGT GCCGCCGGGG AGAGCAAAGT CCTCTGGTTC 
CCAAGGAAAG TGTCCGAGCT GGACAAGTGT CACCACCTCG TCACCAAGTT TGACCCTGAC 
CTGGACTTGG ATCATCCCGG CTTCTCCGAC CAGGCGTACC GCCAGCGCAG GAAGCTGATC 
GCCGAGATCG CCTTCCAGTA CAAGCAAGGC GACCCCATTC CCCACGTGGA GTACACAGCC 
GAGGAGACAG CCACCTGGAA GGAGGTCTAC TCCACGCTGC GGGGCCTGTA CCCCACCCAC 
GCCTGCCGCG AGCACCTGGA GGCCTTCGAG CTGCTGGAGC GCTTCTGCGG GTACCGCGAG 
GACCGAATCC CGCAGCTGGA GGACGTCTCA CGCTTCCTGA AGGAGAGGAC CGGCTTCCAG 
CTGCGGCCCG TGGCCGGCCT GCTGTCCGCG CGGGACTTCC TGGCCAGCCT GGCCTTCCGC 
GTGTTCCAGT GCACCCAGTA CATCCGCCAC GCCTCCTCGC CCATGCACTC CCCGGAGCCG 
GACTGCTGCC ACGAGCTGCT GGGGCACGTG CCCATGCTCG CCGACCGGAC CTTCGCGCAG 
TTTTCCCAGG ACATCGGGCT CGCATCCTTG GGAGTGTCGG ATGAGGAAAT TGAGAAGCTG 
TCCACGCTGT ACTGGTTCAC CGTGGAGTTC GGGCTATGCA AACAGAATGG AGAGGTGAAG 
GCCTACGGCG CGGGGCTGCT GTCCTCCTAC GGGGAGCTCC TGCACTCCCT TTCCGAGGAG 
CCCGAGATCC GGGCCTTCGA CCCTGATGCA GCGGCCGTGC AGCCCTATCA GGACCAGACC 
TACCAGCCCG TCTACTTCGT GTCTGAGAGC TTCAGCGATG CCAAGGACAA GCTCAGGAGC 
TACGCCTCCC GCATCCAGCG CCCCTTCTCT GTGAAGTTTG ACCCGTACAC ACTGGCCATC 
GACGTGCTGG ACAGTCCCCA CGCCATCCGG CGCGCCCTGG ACGGCGTCCA GGACGAGATG 
CAGGCCCTGG CCCATGCTCT GAACGCCATC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842736.1
CDS	385..1860
Translation	MPTPNAASPQAKGFRRAVSELDAKQAEAIMSPRFVGRRQSLIQDARKEREKAEAAASSSESAEAGSLVERDGKAVLTLLFALRPTKPPALTRAIKVFETFEAHLHHLETRPAQPLRAGSPPLECFVRCEVPGPVVPALLSALRRVAEDVRAAGESKVLWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQAYRQRRKLIAEIAFQYKQGDPIPHVEYTAEETATWKEVYSTLRGLYPTHACREHLEAFELLERFCGYREDRIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGVSDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHSLSEEPEIRAFDPDAAAVQPYQDQTYQPVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPHAIRRALDGVQDEMQALAHALNAIS

Target ORF information:

RefSeq Version	XM_024986968.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine hydroxylase (TH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986968.1

ORF Insert Sequence:

ATGCCTACCC CCAACGCCGC CTCGCCGCAG GCCAAGGGCT TCCGCAGGGC CGTCTCCGAG 
CTGGACGCCA AGCAGGCTGA GGCCATCATG TCCCCACGCT TCGTCGGGAG GCGGCAGAGC 
CTCATCCAGG ACGCACGCAA GGAGCGGGAG AAGGCGGAGG CCGCGGCCTC ATCCTCGGAG 
TCCGCGGAGG CAGGCAGCCT GGTGGAGAGG GACGGGAAGG CGGTGCTGAC CCTGCTGTTC 
GCCCTGCGGC CCACCAAGCC CCCTGCGCTG ACCCGGGCTA TCAAGGTGTT TGAGACATTC 
GAAGCCCACC TCCACCACCT GGAGACCCGA CCGGCCCAGC CACTGCGGGC GGGGAGCCCG 
CCCCTGGAGT GCTTCGTGCG CTGTGAGGTG CCCGGCCCAG TGGTGCCCGC CCTGCTGAGC 
GCCCTGCGCC GCGTGGCAGA AGATGTGCGT GCCGCCGGGG AGAGCAAAGT CCTCTGGTTC 
CCAAGGAAAG TGTCCGAGCT GGACAAGTGT CACCACCTCG TCACCAAGTT TGACCCTGAC 
CTGGACTTGG ATCATCCCGG CTTCTCCGAC CAGGCGTACC GCCAGCGCAG GAAGCTGATC 
GCCGAGATCG CCTTCCAGTA CAAGCAAGGC GACCCCATTC CCCACGTGGA GTACACAGCC 
GAGGAGACAG CCACCTGGAA GGAGGTCTAC TCCACGCTGC GGGGCCTGTA CCCCACCCAC 
GCCTGCCGCG AGCACCTGGA GGCCTTCGAG CTGCTGGAGC GCTTCTGCGG GTACCGCGAG 
GACCGAATCC CGCAGCTGGA GGACGTCTCA CGCTTCCTGA AGGAGAGGAC CGGCTTCCAG 
CTGCGGCCCG TGGCCGGCCT GCTGTCCGCG CGGGACTTCC TGGCCAGCCT GGCCTTCCGC 
GTGTTCCAGT GCACCCAGTA CATCCGCCAC GCCTCCTCGC CCATGCACTC CCCGGAGCCG 
GACTGCTGCC ACGAGCTGCT GGGGCACGTG CCCATGCTCG CCGACCGGAC CTTCGCGCAG 
TTTTCCCAGG ACATCGGGCT CGCATCCTTG GGAGTGTCGG ATGAGGAAAT TGAGAAGCTG 
TCCACGCTGT ACTGGTTCAC CGTGGAGTTC GGGCTATGCA AACAGAATGG AGAGGTGAAG 
GCCTACGGCG CGGGGCTGCT GTCCTCCTAC GGGGAGCTCC TGCACTCCCT TTCCGAGGAG 
CCCGAGATCC GGGCCTTCGA CCCTGATGCA GCGGCCGTGC AGCCCTATCA GGACCAGACC 
TACCAGCCCG TCTACTTCGT GTCTGAGAGC TTCAGCGATG CCAAGGACAA GCTCAGGAGC 
TACGCCTCCC GCATCCAGCG CCCCTTCTCT GTGAAGTTTG ACCCGTACAC ACTGGCCATC 
GACGTGCTGG ACAGTCCCCA CGCCATCCGG CGCGCCCTGG ACGGCGTCCA GGACGAGATG 
CAGGCCCTGG CCCATGCTCT GAACGCCATC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TH ( XM_015461329.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316815.1 Bos taurus tyrosine hydroxylase (TH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa231828
Clone ID Related Accession (Same CDS sequence)	XM_015461329.2
Accession Version	XM_015461329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tyrosine 3-monooxygenase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCAC GCTTCGTCGG GAGGCGGCAG AGCCTCATCC AGGACGCACG CAAGGAGCGG 
GAGAAGGCGG AGGCCGCGGC CTCATCCTCG GAGTCCGCGG AGGCAGGCAG CCTGGTGGAG 
AGGGACGGGA AGGCGGTGCT GACCCTGCTG TTCGCCCTGC GGCCCACCAA GCCCCCTGCG 
CTGACCCGGG CTATCAAGGT GTTTGAGACA TTCGAAGCCC ACCTCCACCA CCTGGAGACC 
CGACCGGCCC AGCCACTGCG GGCGGGGAGC CCGCCCCTGG AGTGCTTCGT GCGCTGTGAG 
GTGCCCGGCC CAGTGGTGCC CGCCCTGCTG AGCGCCCTGC GCCGCGTGGC AGAAGATGTG 
CGTGCCGCCG GGGAGAGCAA AGTCCTCTGG TTCCCAAGGA AAGTGTCCGA GCTGGACAAG 
TGTCACCACC TCGTCACCAA GTTTGACCCT GACCTGGACT TGGATCATCC CGGCTTCTCC 
GACCAGGCGT ACCGCCAGCG CAGGAAGCTG ATCGCCGAGA TCGCCTTCCA GTACAAGCAA 
GGCGACCCCA TTCCCCACGT GGAGTACACA GCCGAGGAGA CAGCCACCTG GAAGGAGGTC 
TACTCCACGC TGCGGGGCCT GTACCCCACC CACGCCTGCC GCGAGCACCT GGAGGCCTTC 
GAGCTGCTGG AGCGCTTCTG CGGGTACCGC GAGGACCGAA TCCCGCAGCT GGAGGACGTC 
TCACGCTTCC TGAAGGAGAG GACCGGCTTC CAGCTGCGGC CCGTGGCCGG CCTGCTGTCC 
GCGCGGGACT TCCTGGCCAG CCTGGCCTTC CGCGTGTTCC AGTGCACCCA GTACATCCGC 
CACGCCTCCT CGCCCATGCA CTCCCCGGAG CCGGACTGCT GCCACGAGCT GCTGGGGCAC 
GTGCCCATGC TCGCCGACCG GACCTTCGCG CAGTTTTCCC AGGACATCGG GCTCGCATCC 
TTGGGAGTGT CGGATGAGGA AATTGAGAAG CTGTCCACGC TGTACTGGTT CACCGTGGAG 
TTCGGGCTAT GCAAACAGAA TGGAGAGGTG AAGGCCTACG GCGCGGGGCT GCTGTCCTCC 
TACGGGGAGC TCCTGCACTC CCTTTCCGAG GAGCCCGAGA TCCGGGCCTT CGACCCTGAT 
GCAGCGGCCG TGCAGCCCTA TCAGGACCAG ACCTACCAGC CCGTCTACTT CGTGTCTGAG 
AGCTTCAGCG ATGCCAAGGA CAAGCTCAGG AGCTACGCCT CCCGCATCCA GCGCCCCTTC 
TCTGTGAAGT TTGACCCGTA CACACTGGCC ATCGACGTGC TGGACAGTCC CCACGCCATC 
CGGCGCGCCC TGGACGGCGT CCAGGACGAG ATGCAGGCCC TGGCCCATGC TCTGAACGCC 
ATCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316815.1
CDS	312..1700
Translation	MSPRFVGRRQSLIQDARKEREKAEAAASSSESAEAGSLVERDGKAVLTLLFALRPTKPPALTRAIKVFETFEAHLHHLETRPAQPLRAGSPPLECFVRCEVPGPVVPALLSALRRVAEDVRAAGESKVLWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQAYRQRRKLIAEIAFQYKQGDPIPHVEYTAEETATWKEVYSTLRGLYPTHACREHLEAFELLERFCGYREDRIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGVSDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHSLSEEPEIRAFDPDAAAVQPYQDQTYQPVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPHAIRRALDGVQDEMQALAHALNAIS

Target ORF information:

RefSeq Version	XM_015461329.2
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine hydroxylase (TH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461329.2

ORF Insert Sequence:

ATGTCCCCAC GCTTCGTCGG GAGGCGGCAG AGCCTCATCC AGGACGCACG CAAGGAGCGG 
GAGAAGGCGG AGGCCGCGGC CTCATCCTCG GAGTCCGCGG AGGCAGGCAG CCTGGTGGAG 
AGGGACGGGA AGGCGGTGCT GACCCTGCTG TTCGCCCTGC GGCCCACCAA GCCCCCTGCG 
CTGACCCGGG CTATCAAGGT GTTTGAGACA TTCGAAGCCC ACCTCCACCA CCTGGAGACC 
CGACCGGCCC AGCCACTGCG GGCGGGGAGC CCGCCCCTGG AGTGCTTCGT GCGCTGTGAG 
GTGCCCGGCC CAGTGGTGCC CGCCCTGCTG AGCGCCCTGC GCCGCGTGGC AGAAGATGTG 
CGTGCCGCCG GGGAGAGCAA AGTCCTCTGG TTCCCAAGGA AAGTGTCCGA GCTGGACAAG 
TGTCACCACC TCGTCACCAA GTTTGACCCT GACCTGGACT TGGATCATCC CGGCTTCTCC 
GACCAGGCGT ACCGCCAGCG CAGGAAGCTG ATCGCCGAGA TCGCCTTCCA GTACAAGCAA 
GGCGACCCCA TTCCCCACGT GGAGTACACA GCCGAGGAGA CAGCCACCTG GAAGGAGGTC 
TACTCCACGC TGCGGGGCCT GTACCCCACC CACGCCTGCC GCGAGCACCT GGAGGCCTTC 
GAGCTGCTGG AGCGCTTCTG CGGGTACCGC GAGGACCGAA TCCCGCAGCT GGAGGACGTC 
TCACGCTTCC TGAAGGAGAG GACCGGCTTC CAGCTGCGGC CCGTGGCCGG CCTGCTGTCC 
GCGCGGGACT TCCTGGCCAG CCTGGCCTTC CGCGTGTTCC AGTGCACCCA GTACATCCGC 
CACGCCTCCT CGCCCATGCA CTCCCCGGAG CCGGACTGCT GCCACGAGCT GCTGGGGCAC 
GTGCCCATGC TCGCCGACCG GACCTTCGCG CAGTTTTCCC AGGACATCGG GCTCGCATCC 
TTGGGAGTGT CGGATGAGGA AATTGAGAAG CTGTCCACGC TGTACTGGTT CACCGTGGAG 
TTCGGGCTAT GCAAACAGAA TGGAGAGGTG AAGGCCTACG GCGCGGGGCT GCTGTCCTCC 
TACGGGGAGC TCCTGCACTC CCTTTCCGAG GAGCCCGAGA TCCGGGCCTT CGACCCTGAT 
GCAGCGGCCG TGCAGCCCTA TCAGGACCAG ACCTACCAGC CCGTCTACTT CGTGTCTGAG 
AGCTTCAGCG ATGCCAAGGA CAAGCTCAGG AGCTACGCCT CCCGCATCCA GCGCCCCTTC 
TCTGTGAAGT TTGACCCGTA CACACTGGCC ATCGACGTGC TGGACAGTCC CCACGCCATC 
CGGCGCGCCC TGGACGGCGT CCAGGACGAG ATGCAGGCCC TGGCCCATGC TCTGAACGCC 
ATCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TH ( XM_015461329.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015316815.1 Bos taurus tyrosine hydroxylase (TH), transcript variant X1, mRNA.

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CloneID	OBa89144
Clone ID Related Accession (Same CDS sequence)	XM_015461329.1
Accession Version	XM_015461329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine 3-monooxygenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCAC GCTTCGTCGG GAGGCGGCAG AGCCTCATCC AGGACGCACG CAAGGAGCGG 
GAGAAGGCGG AGGCCGCGGC CTCATCCTCG GAGTCCGCGG AGGCGGGCAG CCTGGTGGAG 
AGGGACGGGA AGGCGGTGCT GACCCTGCTG TTCGCCCTGC GGCCCACCAA GCCCCCTGCG 
CTGACCCGGG CTATCAAGGT GTTTGAGACA TTCGAAGCCC ACCTCCACCA CCTGGAGACC 
CGACCGGCCC AGCCACTGCG GGCGGGGAGC CCGCCCCTGG AGTGCTTCGT GCGCTGTGAG 
GTGCCCGGCC CAGTGGTGCC CGCCCTGCTG AGCGCCCTGC GCCGCGTGGC AGAAGATGTG 
CGTGCCGCCG GGGAGAGCAA AGTCCTCTGG TTCCCAAGGA AAGTGTCCGA GCTGGACAAG 
TGTCACCACC TCGTCACCAA GTTTGACCCT GACCTGGACT TGGATCATCC CGGCTTCTCC 
GACCAGGCGT ACCGCCAGCG CAGGAAGCTG ATCGCCGAGA TCGCCTTCCA GTACAAGCAA 
GGCGACCCCA TTCCCCACGT GGAGTACACA GCCGAGGAGA CAGCCACCTG GAAGGAGGTC 
TACTCCACGC TGCGGGGCCT GTACCCCACC CACGCCTGCC GCGAGCACCT GGAGGCCTTC 
GAGCTGCTGG AGCGCTTCTG CGGGTACCGC GAGGACCGAA TCCCGCAGCT GGAGGACGTC 
TCACGCTTCC TGAAGGAGAG GACCGGCTTC CAGCTGCGGC CCGTGGCCGG CCTGCTGTCC 
GCGCGGGACT TCCTGGCCAG CCTGGCCTTC CGCGTGTTCC AGTGCACCCA GTACATCCGC 
CACGCCTCCT CGCCCATGCA CTCCCCGGAG CCGGACTGCT GCCACGAGCT GCTGGGGCAC 
GTGCCCATGC TCGCCGACCG GACCTTCGCG CAGTTTTCCC AGGACATCGG GCTCGCATCC 
TTGGGAGTGT CGGATGAGGA AATTGAGAAG CTGTCCACGC TGTACTGGTT CACCGTGGAG 
TTCGGGCTAT GCAAACAGAA TGGAGAGGTG AAGGCCTACG GCGCGGGGCT GCTGTCCTCC 
TACGGGGAGC TCCTGCACTC CCTTTCCGAG GAGCCCGAGA TCCGGGCCTT CGACCCTGAT 
GCAGCGGCCG TGCAGCCCTA TCAGGACCAG ACCTACCAGC CCGTCTACTT CGTGTCTGAG 
AGCTTCAGCG ATGCCAAGGA CAAGCTCAGG AGCTACGCCT CCCGCATCCA GCGCCCCTTC 
TCTGTGAAGT TTGACCCGTA CACACTGGCC ATCGACGTGC TGGACAGTCC CCACGCCATC 
CGGCGCGCCC TGGACGGCGT CCAGGACGAG ATGCAGGCCC TGGCCCATGC TCTGAACGCC 
ATCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015316815.1
CDS	295..1683
Translation	MSPRFVGRRQSLIQDARKEREKAEAAASSSESAEAGSLVERDGKAVLTLLFALRPTKPPALTRAIKVFETFEAHLHHLETRPAQPLRAGSPPLECFVRCEVPGPVVPALLSALRRVAEDVRAAGESKVLWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQAYRQRRKLIAEIAFQYKQGDPIPHVEYTAEETATWKEVYSTLRGLYPTHACREHLEAFELLERFCGYREDRIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGVSDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHSLSEEPEIRAFDPDAAAVQPYQDQTYQPVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPHAIRRALDGVQDEMQALAHALNAIS

Target ORF information:

RefSeq Version	XM_015461329.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tyrosine hydroxylase (TH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015461329.1

ORF Insert Sequence:

ATGTCCCCAC GCTTCGTCGG GAGGCGGCAG AGCCTCATCC AGGACGCACG CAAGGAGCGG 
GAGAAGGCGG AGGCCGCGGC CTCATCCTCG GAGTCCGCGG AGGCGGGCAG CCTGGTGGAG 
AGGGACGGGA AGGCGGTGCT GACCCTGCTG TTCGCCCTGC GGCCCACCAA GCCCCCTGCG 
CTGACCCGGG CTATCAAGGT GTTTGAGACA TTCGAAGCCC ACCTCCACCA CCTGGAGACC 
CGACCGGCCC AGCCACTGCG GGCGGGGAGC CCGCCCCTGG AGTGCTTCGT GCGCTGTGAG 
GTGCCCGGCC CAGTGGTGCC CGCCCTGCTG AGCGCCCTGC GCCGCGTGGC AGAAGATGTG 
CGTGCCGCCG GGGAGAGCAA AGTCCTCTGG TTCCCAAGGA AAGTGTCCGA GCTGGACAAG 
TGTCACCACC TCGTCACCAA GTTTGACCCT GACCTGGACT TGGATCATCC CGGCTTCTCC 
GACCAGGCGT ACCGCCAGCG CAGGAAGCTG ATCGCCGAGA TCGCCTTCCA GTACAAGCAA 
GGCGACCCCA TTCCCCACGT GGAGTACACA GCCGAGGAGA CAGCCACCTG GAAGGAGGTC 
TACTCCACGC TGCGGGGCCT GTACCCCACC CACGCCTGCC GCGAGCACCT GGAGGCCTTC 
GAGCTGCTGG AGCGCTTCTG CGGGTACCGC GAGGACCGAA TCCCGCAGCT GGAGGACGTC 
TCACGCTTCC TGAAGGAGAG GACCGGCTTC CAGCTGCGGC CCGTGGCCGG CCTGCTGTCC 
GCGCGGGACT TCCTGGCCAG CCTGGCCTTC CGCGTGTTCC AGTGCACCCA GTACATCCGC 
CACGCCTCCT CGCCCATGCA CTCCCCGGAG CCGGACTGCT GCCACGAGCT GCTGGGGCAC 
GTGCCCATGC TCGCCGACCG GACCTTCGCG CAGTTTTCCC AGGACATCGG GCTCGCATCC 
TTGGGAGTGT CGGATGAGGA AATTGAGAAG CTGTCCACGC TGTACTGGTT CACCGTGGAG 
TTCGGGCTAT GCAAACAGAA TGGAGAGGTG AAGGCCTACG GCGCGGGGCT GCTGTCCTCC 
TACGGGGAGC TCCTGCACTC CCTTTCCGAG GAGCCCGAGA TCCGGGCCTT CGACCCTGAT 
GCAGCGGCCG TGCAGCCCTA TCAGGACCAG ACCTACCAGC CCGTCTACTT CGTGTCTGAG 
AGCTTCAGCG ATGCCAAGGA CAAGCTCAGG AGCTACGCCT CCCGCATCCA GCGCCCCTTC 
TCTGTGAAGT TTGACCCGTA CACACTGGCC ATCGACGTGC TGGACAGTCC CCACGCCATC 
CGGCGCGCCC TGGACGGCGT CCAGGACGAG ATGCAGGCCC TGGCCCATGC TCTGAACGCC 
ATCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of tyrosine hydroxylase is preserved across different homo- and heterodimeric 14-3-3 proteins.
Amino acids48(5)1221-9(2016 May)
Ghorbani S,Fossbakk A,Jorge-Finnigan A,Flydal MI,Haavik J,Kleppe R

Interferon-alpha signalling in bovine adrenal chromaffin cells: involvement of signal-transducer and activator of transcription 1 and 2, extracellular signal-regulated protein kinases 1/2 and serine 31 phosphorylation of tyrosine hydroxylase.
Journal of neuroendocrinology21(3)200-7(2009 Mar)
Douglas SA,Bunn SJ

Retinol activates tyrosine hydroxylase acutely by increasing the phosphorylation of serine40 and then serine31 in bovine adrenal chromaffin cells.
Journal of neurochemistry103(6)2369-79(2007 Dec)
Gelain DP,Moreira JC,Bevilaqua LR,Dickson PW,Dunkley PR

PACAP stimulates the sustained phosphorylation of tyrosine hydroxylase at serine 40.
Cellular signalling19(6)1141-9(2007 Jun)
Bobrovskaya L,Gelain DP,Gilligan C,Dickson PW,Dunkley PR

Predicted amino acid sequence of bovine tyrosine hydroxylase and its similarity to tyrosine hydroxylases from other species.
Journal of neurochemistry51(2)572-8(1988 Aug)
Saadat S,Stehle AD,Lamouroux A,Mallet J,Thoenen H

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TH cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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