ENGASE gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005221289.3	XP_005221346.1	cytosolic endo-beta-N-acetylglucosaminidase isoform X1
XM_003583636.4	XP_003583684.3	cytosolic endo-beta-N-acetylglucosaminidase isoform X2
XM_010824291.2	XP_010822593.2	cytosolic endo-beta-N-acetylglucosaminidase isoform X3
XM_005221289.4	XP_005221346.2	cytosolic endo-beta-N-acetylglucosaminidase isoform X1
XM_003587480.5	XP_003587528.3	cytosolic endo-beta-N-acetylglucosaminidase isoform X2
XM_010816579.3	XP_010814881.2	cytosolic endo-beta-N-acetylglucosaminidase isoform X4
XM_003587480.4	XP_003587528.2	cytosolic endo-beta-N-acetylglucosaminidase isoform X2
XM_024980999.1	XP_024836767.1	cytosolic endo-beta-N-acetylglucosaminidase isoform X3
XM_010816579.2	XP_010814881.1	cytosolic endo-beta-N-acetylglucosaminidase isoform X3
XM_005195832.3	XP_005195889.2	cytosolic endo-beta-N-acetylglucosaminidase isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa60348	XM_010816579.2 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa60348	XM_010824291.2 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa60347	XM_010816579.3 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa10406	XM_005221289.4 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa10407	XM_003587480.5 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa10408	XM_024980999.1 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa30442	XM_005195832.3 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa30442	XM_005221289.3 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa30443	XM_003587480.4 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa30443	XM_003583636.4 Latest version!	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ENGASE ( XM_010824291.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822593.2 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa60348
Clone ID Related Accession (Same CDS sequence)	XM_010824291.2 , XM_010816579.2
Accession Version	XM_010824291.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCATG ACATGATGGG CGGGTACCTG GATGACAAGT TTATTCAAGG CTCAGCCACA 
CAGACTCCCT ATTCCTTTTA CCACTGGCAG TACATCGACA TCTTTGTGTA CTTCAGCCAT 
CACACGGTCA CCATCCCCCC AGTAGGCTGG ACCAACGCTG CCCACAGGCA TGGGGTCTGT 
GTGCTGGGGA CTTTCATCAC TGAATGGAAA GACGGGGAGC GGCTCTGCCA GGCCTTCCTG 
GCTGGGGATG AGCACTCATA CCGGGCGGTG GCCGATCAGC TGGTCCTGAT CGCCCAGTTT 
TTCCGATTTG ACGGCTGGCT GATCAACATT GAGAACTCTC TGAGTCTGGC CGCCGTGGGG 
AACGTGCCCC ACTTCCTCCA GTACCTGACC TCTCAGCTGC ATCAGCAGGT CCCCGGGGGC 
CTGGTGCTCT GGTATGACAG CGTGGTGAGC AGCGGGCAGC TCAAATGGCA GAACGAGCTC 
AACGAGCAGA ACAGAGTCTT CTTTGATGCC TGCGACGGCT TCTTCACCAA CTATAACTGG 
CGGGAGGAGC ATCTGGAGCG GACGCTGGGG CAGGCCGGGG AGCGCCGGGC GGACGTGTAC 
GTGGGTGTAG ACGTGTTCGC CCGCGGGAAT GTCGTCGGGG GCCGGTTCGA CACGAATAAG 
TCGCTGGAGC TGATTCGGAA GCACGGGTTC TCGGCGGCCC TGTTTGCGCC TGGCTGGGTG 
TACGAGTGTT TGGAGAAGGG GAATTTCTTC CAGAACCAGG ACAAGTTCTG GAGTTTGCTG 
GAACGCTACT TGCCCACACA CAGCATCTGC TCCTTGCCCT TTGTCACTTC CTTCTGCCTG 
GGCATGGGGA CTCGAAGAGT CTACTATGGC CAGGAGGAGG CCGTGGGGCC CTGGTACCAC 
CCGAGCGCCC AGGAGATCCA GCCCCTGTTT GGAGAGCACA GGCTGGAAGG GGACGGGCAG 
GGCTGGGTGA AGACGCACTG CTGCCTGGAG GACTCCTGGA ACGGGGGCAG CTCTCTGCTC 
ATCCGAGGGC TGATTCCGCC CGAGGTTGGA AACGTGGCTG TGAGGTTATT CTCCCTGCAG 
GTCCCAGTGC CACCCAAGAT CTTCGTGTCC CTGGTGTACA AGCTGGAAGG GCCTTCGGCG 
GTGGGGGTGG CTTTGGAGCT CACCACAGGG GACGCGGGCA GCTGTCATGT GGGAGGCATC 
TCGGTGCTGA GCGCAGAAAC GAGCTCAAGG CGCAGTCCCC GACCCCTCCG GGTGCCCCCA 
ACCAAGCTGG CCAAATGGGT GGGCCGTTGC GGCCAGCAGC TCAGTGGGGG CTGGGTCCAG 
CGCTGCTACG AAGTGACTCT GCGGGGCTGC CTCCTACAGG ACCTCTTTGT TAATTTCTCC 
CGGCCTCCGG GCAGCCAGAA GGAGGAGAAC TTCATCTGTC GCCTTGGAGA GCTCCAGGTG 
GTGGATGCCA ACAGCCTGCT GACCCCGCTC CCCCAGGTGC AGGCCGTGAC GGTCTCGCAT 
GTGCGCTGGC AGCCGTTCGC CTCCGAGTGG GAGGGCGGCC CCGCCGGGCT GCGGCTCAGC 
TGTACCCTCC ACTGGGCCTA CCTCCTTCCC CGTGTCCGGT GCTTCCGGAT CCACTGCTGC 
CGAGGATCGG AAGGTGGCTC TCCCAGCAGC GGGCCTTCGC AGCCAGGGAG GCCCAGGCTG 
CTGGGCCTGG CTTTCGTCAA CCGGTATCGG ATAGTGGGCC TGGCAGTGGC AGCCGCGGAG 
CCTGGCCAGG ATGGCCGCGT GGAGTTCCTG GTGGAGCCTG TCCCCAAAGA GGGGTTCCTG 
GTGCCTCAGG CCGAGTGGGG CAAGGCGGCC ATACTCTACT CCATGCCCCG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822593.2
CDS	248..2104
Translation	MCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDSWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSAETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_010824291.2
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824291.2

ORF Insert Sequence:

ATGTGTCATG ACATGATGGG CGGGTACCTG GATGACAAGT TTATTCAAGG CTCAGCCACA 
CAGACTCCCT ATTCCTTTTA CCACTGGCAG TACATCGACA TCTTTGTGTA CTTCAGCCAT 
CACACGGTCA CCATCCCCCC AGTAGGCTGG ACCAACGCTG CCCACAGGCA TGGGGTCTGT 
GTGCTGGGGA CTTTCATCAC TGAATGGAAA GACGGGGAGC GGCTCTGCCA GGCCTTCCTG 
GCTGGGGATG AGCACTCATA CCGGGCGGTG GCCGATCAGC TGGTCCTGAT CGCCCAGTTT 
TTCCGATTTG ACGGCTGGCT GATCAACATT GAGAACTCTC TGAGTCTGGC CGCCGTGGGG 
AACGTGCCCC ACTTCCTCCA GTACCTGACC TCTCAGCTGC ATCAGCAGGT CCCCGGGGGC 
CTGGTGCTCT GGTATGACAG CGTGGTGAGC AGCGGGCAGC TCAAATGGCA GAACGAGCTC 
AACGAGCAGA ACAGAGTCTT CTTTGATGCC TGCGACGGCT TCTTCACCAA CTATAACTGG 
CGGGAGGAGC ATCTGGAGCG GACGCTGGGG CAGGCCGGGG AGCGCCGGGC GGACGTGTAC 
GTGGGTGTAG ACGTGTTCGC CCGCGGGAAT GTCGTCGGGG GCCGGTTCGA CACGAATAAG 
TCGCTGGAGC TGATTCGGAA GCACGGGTTC TCGGCGGCCC TGTTTGCGCC TGGCTGGGTG 
TACGAGTGTT TGGAGAAGGG GAATTTCTTC CAGAACCAGG ACAAGTTCTG GAGTTTGCTG 
GAACGCTACT TGCCCACACA CAGCATCTGC TCCTTGCCCT TTGTCACTTC CTTCTGCCTG 
GGCATGGGGA CTCGAAGAGT CTACTATGGC CAGGAGGAGG CCGTGGGGCC CTGGTACCAC 
CCGAGCGCCC AGGAGATCCA GCCCCTGTTT GGAGAGCACA GGCTGGAAGG GGACGGGCAG 
GGCTGGGTGA AGACGCACTG CTGCCTGGAG GACTCCTGGA ACGGGGGCAG CTCTCTGCTC 
ATCCGAGGGC TGATTCCGCC CGAGGTTGGA AACGTGGCTG TGAGGTTATT CTCCCTGCAG 
GTCCCAGTGC CACCCAAGAT CTTCGTGTCC CTGGTGTACA AGCTGGAAGG GCCTTCGGCG 
GTGGGGGTGG CTTTGGAGCT CACCACAGGG GACGCGGGCA GCTGTCATGT GGGAGGCATC 
TCGGTGCTGA GCGCAGAAAC GAGCTCAAGG CGCAGTCCCC GACCCCTCCG GGTGCCCCCA 
ACCAAGCTGG CCAAATGGGT GGGCCGTTGC GGCCAGCAGC TCAGTGGGGG CTGGGTCCAG 
CGCTGCTACG AAGTGACTCT GCGGGGCTGC CTCCTACAGG ACCTCTTTGT TAATTTCTCC 
CGGCCTCCGG GCAGCCAGAA GGAGGAGAAC TTCATCTGTC GCCTTGGAGA GCTCCAGGTG 
GTGGATGCCA ACAGCCTGCT GACCCCGCTC CCCCAGGTGC AGGCCGTGAC GGTCTCGCAT 
GTGCGCTGGC AGCCGTTCGC CTCCGAGTGG GAGGGCGGCC CCGCCGGGCT GCGGCTCAGC 
TGTACCCTCC ACTGGGCCTA CCTCCTTCCC CGTGTCCGGT GCTTCCGGAT CCACTGCTGC 
CGAGGATCGG AAGGTGGCTC TCCCAGCAGC GGGCCTTCGC AGCCAGGGAG GCCCAGGCTG 
CTGGGCCTGG CTTTCGTCAA CCGGTATCGG ATAGTGGGCC TGGCAGTGGC AGCCGCGGAG 
CCTGGCCAGG ATGGCCGCGT GGAGTTCCTG GTGGAGCCTG TCCCCAAAGA GGGGTTCCTG 
GTGCCTCAGG CCGAGTGGGG CAAGGCGGCC ATACTCTACT CCATGCCCCG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_010816579.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814881.1 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa60348
Clone ID Related Accession (Same CDS sequence)	XM_010824291.2 , XM_010816579.2
Accession Version	XM_010816579.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816579.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCATG ACATGATGGG CGGGTACCTG GATGACAAGT TTATTCAAGG CTCAGCCACA 
CAGACTCCCT ATTCCTTTTA CCACTGGCAG TACATCGACA TCTTTGTGTA CTTCAGCCAT 
CACACGGTCA CCATCCCCCC AGTAGGCTGG ACCAACGCTG CCCACAGGCA TGGGGTCTGT 
GTGCTGGGGA CTTTCATCAC TGAATGGAAA GACGGGGAGC GGCTCTGCCA GGCCTTCCTG 
GCTGGGGATG AGCACTCATA CCGGGCGGTG GCCGATCAGC TGGTCCTGAT CGCCCAGTTT 
TTCCGATTTG ACGGCTGGCT GATCAACATT GAGAACTCTC TGAGTCTGGC CGCCGTGGGG 
AACGTGCCCC ACTTCCTCCA GTACCTGACC TCTCAGCTGC ATCAGCAGGT CCCCGGGGGC 
CTGGTGCTCT GGTATGACAG CGTGGTGAGC AGCGGGCAGC TCAAATGGCA GAACGAGCTC 
AACGAGCAGA ACAGAGTCTT CTTTGATGCC TGCGACGGCT TCTTCACCAA CTATAACTGG 
CGGGAGGAGC ATCTGGAGCG GACGCTGGGG CAGGCCGGGG AGCGCCGGGC GGACGTGTAC 
GTGGGTGTAG ACGTGTTCGC CCGCGGGAAT GTCGTCGGGG GCCGGTTCGA CACGAATAAG 
TCGCTGGAGC TGATTCGGAA GCACGGGTTC TCGGCGGCCC TGTTTGCGCC TGGCTGGGTG 
TACGAGTGTT TGGAGAAGGG GAATTTCTTC CAGAACCAGG ACAAGTTCTG GAGTTTGCTG 
GAACGCTACT TGCCCACACA CAGCATCTGC TCCTTGCCCT TTGTCACTTC CTTCTGCCTG 
GGCATGGGGA CTCGAAGAGT CTACTATGGC CAGGAGGAGG CCGTGGGGCC CTGGTACCAC 
CCGAGCGCCC AGGAGATCCA GCCCCTGTTT GGAGAGCACA GGCTGGAAGG GGACGGGCAG 
GGCTGGGTGA AGACGCACTG CTGCCTGGAG GACTCCTGGA ACGGGGGCAG CTCTCTGCTC 
ATCCGAGGGC TGATTCCGCC CGAGGTTGGA AACGTGGCTG TGAGGTTATT CTCCCTGCAG 
GTCCCAGTGC CACCCAAGAT CTTCGTGTCC CTGGTGTACA AGCTGGAAGG GCCTTCGGCG 
GTGGGGGTGG CTTTGGAGCT CACCACAGGG GACGCGGGCA GCTGTCATGT GGGAGGCATC 
TCGGTGCTGA GCGCAGAAAC GAGCTCAAGG CGCAGTCCCC GACCCCTCCG GGTGCCCCCA 
ACCAAGCTGG CCAAATGGGT GGGCCGTTGC GGCCAGCAGC TCAGTGGGGG CTGGGTCCAG 
CGCTGCTACG AAGTGACTCT GCGGGGCTGC CTCCTACAGG ACCTCTTTGT TAATTTCTCC 
CGGCCTCCGG GCAGCCAGAA GGAGGAGAAC TTCATCTGTC GCCTTGGAGA GCTCCAGGTG 
GTGGATGCCA ACAGCCTGCT GACCCCGCTC CCCCAGGTGC AGGCCGTGAC GGTCTCGCAT 
GTGCGCTGGC AGCCGTTCGC CTCCGAGTGG GAGGGCGGCC CCGCCGGGCT GCGGCTCAGC 
TGTACCCTCC ACTGGGCCTA CCTCCTTCCC CGTGTCCGGT GCTTCCGGAT CCACTGCTGC 
CGAGGATCGG AAGGTGGCTC TCCCAGCAGC GGGCCTTCGC AGCCAGGGAG GCCCAGGCTG 
CTGGGCCTGG CTTTCGTCAA CCGGTATCGG ATAGTGGGCC TGGCAGTGGC AGCCGCGGAG 
CCTGGCCAGG ATGGCCGCGT GGAGTTCCTG GTGGAGCCTG TCCCCAAAGA GGGGTTCCTG 
GTGCCTCAGG CCGAGTGGGG CAAGGCGGCC ATACTCTACT CCATGCCCCG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814881.1
CDS	248..2104
Translation	MCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDSWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSAETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_010816579.2
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816579.2

ORF Insert Sequence:

ATGTGTCATG ACATGATGGG CGGGTACCTG GATGACAAGT TTATTCAAGG CTCAGCCACA 
CAGACTCCCT ATTCCTTTTA CCACTGGCAG TACATCGACA TCTTTGTGTA CTTCAGCCAT 
CACACGGTCA CCATCCCCCC AGTAGGCTGG ACCAACGCTG CCCACAGGCA TGGGGTCTGT 
GTGCTGGGGA CTTTCATCAC TGAATGGAAA GACGGGGAGC GGCTCTGCCA GGCCTTCCTG 
GCTGGGGATG AGCACTCATA CCGGGCGGTG GCCGATCAGC TGGTCCTGAT CGCCCAGTTT 
TTCCGATTTG ACGGCTGGCT GATCAACATT GAGAACTCTC TGAGTCTGGC CGCCGTGGGG 
AACGTGCCCC ACTTCCTCCA GTACCTGACC TCTCAGCTGC ATCAGCAGGT CCCCGGGGGC 
CTGGTGCTCT GGTATGACAG CGTGGTGAGC AGCGGGCAGC TCAAATGGCA GAACGAGCTC 
AACGAGCAGA ACAGAGTCTT CTTTGATGCC TGCGACGGCT TCTTCACCAA CTATAACTGG 
CGGGAGGAGC ATCTGGAGCG GACGCTGGGG CAGGCCGGGG AGCGCCGGGC GGACGTGTAC 
GTGGGTGTAG ACGTGTTCGC CCGCGGGAAT GTCGTCGGGG GCCGGTTCGA CACGAATAAG 
TCGCTGGAGC TGATTCGGAA GCACGGGTTC TCGGCGGCCC TGTTTGCGCC TGGCTGGGTG 
TACGAGTGTT TGGAGAAGGG GAATTTCTTC CAGAACCAGG ACAAGTTCTG GAGTTTGCTG 
GAACGCTACT TGCCCACACA CAGCATCTGC TCCTTGCCCT TTGTCACTTC CTTCTGCCTG 
GGCATGGGGA CTCGAAGAGT CTACTATGGC CAGGAGGAGG CCGTGGGGCC CTGGTACCAC 
CCGAGCGCCC AGGAGATCCA GCCCCTGTTT GGAGAGCACA GGCTGGAAGG GGACGGGCAG 
GGCTGGGTGA AGACGCACTG CTGCCTGGAG GACTCCTGGA ACGGGGGCAG CTCTCTGCTC 
ATCCGAGGGC TGATTCCGCC CGAGGTTGGA AACGTGGCTG TGAGGTTATT CTCCCTGCAG 
GTCCCAGTGC CACCCAAGAT CTTCGTGTCC CTGGTGTACA AGCTGGAAGG GCCTTCGGCG 
GTGGGGGTGG CTTTGGAGCT CACCACAGGG GACGCGGGCA GCTGTCATGT GGGAGGCATC 
TCGGTGCTGA GCGCAGAAAC GAGCTCAAGG CGCAGTCCCC GACCCCTCCG GGTGCCCCCA 
ACCAAGCTGG CCAAATGGGT GGGCCGTTGC GGCCAGCAGC TCAGTGGGGG CTGGGTCCAG 
CGCTGCTACG AAGTGACTCT GCGGGGCTGC CTCCTACAGG ACCTCTTTGT TAATTTCTCC 
CGGCCTCCGG GCAGCCAGAA GGAGGAGAAC TTCATCTGTC GCCTTGGAGA GCTCCAGGTG 
GTGGATGCCA ACAGCCTGCT GACCCCGCTC CCCCAGGTGC AGGCCGTGAC GGTCTCGCAT 
GTGCGCTGGC AGCCGTTCGC CTCCGAGTGG GAGGGCGGCC CCGCCGGGCT GCGGCTCAGC 
TGTACCCTCC ACTGGGCCTA CCTCCTTCCC CGTGTCCGGT GCTTCCGGAT CCACTGCTGC 
CGAGGATCGG AAGGTGGCTC TCCCAGCAGC GGGCCTTCGC AGCCAGGGAG GCCCAGGCTG 
CTGGGCCTGG CTTTCGTCAA CCGGTATCGG ATAGTGGGCC TGGCAGTGGC AGCCGCGGAG 
CCTGGCCAGG ATGGCCGCGT GGAGTTCCTG GTGGAGCCTG TCCCCAAAGA GGGGTTCCTG 
GTGCCTCAGG CCGAGTGGGG CAAGGCGGCC ATACTCTACT CCATGCCCCG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_010816579.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814881.2 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa60347
Clone ID Related Accession (Same CDS sequence)	XM_010816579.3
Accession Version	XM_010816579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCATG ACATGATGGG CGGGTACCTG GATGACAAGT TTATTCAAGG CTCAGCCACA 
CAGACTCCCT ATTCCTTTTA CCACTGGCAG TACATCGACA TCTTTGTGTA CTTCAGCCAT 
CACACGGTCA CCATCCCCCC AGTAGGCTGG ACCAACGCTG CCCACAGGCA TGGGGTCTGT 
GTGCTGGGGA CTTTCATCAC TGAATGGAAA GACGGGGAGC GGCTCTGCCA GGCCTTCCTG 
GCTGGGGATG AGCACTCATA CCGGGCGGTG GCCGATCAGC TGGTCCTGAT CGCCCAGTTT 
TTCCGATTTG ACGGCTGGCT GATCAACATT GAGAACTCTC TGAGTCTGGC CGCCGTGGGG 
AACGTGCCCC ACTTCCTCCA GTACCTGACC TCTCAGCTGC ATCAGCAGGT CCCCGGGGGC 
CTGGTGCTCT GGTATGACAG CGTGGTGAGC AGCGGGCAGC TCAAATGGCA GAACGAGCTC 
AACGAGCAGA ACAGAGTCTT CTTTGATGCC TGCGACGGCT TCTTCACCAA CTATAACTGG 
CGGGAGGAGC ATCTGGAGCG GACGCTGGGG CAGGCCGGGG AGCGCCGGGC GGACGTGTAC 
GTGGGTGTAG ACGTGTTCGC CCGCGGGAAT GTCGTCGGGG GCCGGTTCGA CACGAATAAG 
TCGCTGGAGC TGATTCGGAA GCACGGGTTC TCGGCGGCCC TGTTTGCGCC TGGCTGGGTG 
TACGAGTGTT TGGAGAAGGG GAATTTCTTC CAGAACCAGG ACAAGTTCTG GAGTTTGCTG 
GAACGCTACT TGCCCACACA CAGCATCTGC TCCTTGCCCT TTGTCACTTC CTTCTGCCTG 
GGCATGGGGA CTCGAAGAGT CTACTATGGC CAGGAGGAGG CCGTGGGGCC CTGGTACCAC 
CCGAGCGCCC AGGAGATCCA GCCCCTGTTT GGAGAGCACA GGCTGGAAGG GGACGGGCAG 
GGCTGGGTGA AGACGCACTG CTGCCTGGAG GACGCCTGGA ACGGGGGCAG CTCTCTGCTC 
ATCCGAGGGC TGATTCCGCC CGAGGTTGGA AACGTGGCTG TGAGGTTATT CTCCCTGCAG 
GTCCCAGTGC CACCCAAGAT CTTCGTGTCC CTGGTGTACA AGCTGGAAGG GCCTTCGGCG 
GTGGGGGTGG CTTTGGAGCT CACCACAGGG GACGCGGGCA GCTGTCATGT GGGAGGCATC 
TCGGTGCTGA GCGCAGAAAC GAGCTCAAGG CGCAGTCCCC GACCCCTCCG GGTGCCCCCA 
ACCAAGCTGG CCAAATGGGT GGGCCGTTGC GGCCAGCAGC TCAGTGGGGG CTGGGTCCAG 
CGCTGCTACG AAGTGACTCT GCGGGGCTGC CTCCTACAGG ACCTCTTTGT TAATTTCTCC 
CGGCCTCCGG GCAGCCAGAA GGAGGAGAAC TTCATCTGTC GCCTTGGAGA GCTCCAGGTG 
GTGGATGCCA ACAGCCTGCT GACCCCGCTC CCCCAGGTGC AGGCCGTGAC GGTCTCGCAT 
GTGCGCTGGC AGCCGTTCGC CTCCGAGTGG GAGGGCGGCC CCGCCGGGCT GCGGCTCAGC 
TGTACCCTCC ACTGGGCCTA CCTCCTTCCC CGTGTCCGGT GCTTCCGGAT CCACTGCTGC 
CGAGGATCGG AAGGTGGCTC TCCCAGCAGC GGGCCTTCGC AGCCAGGGAG GCCCAGGCTG 
CTGGGCCTGG CTTTCGTCAA CCGGTATCGG ATAGTGGGCC TGGCAGTGGC AGCCGCGGAG 
CCTGGCCAGG ATGGCCGCGT GGAGTTCCTG GTGGAGCCTG TCCCCAAAGA GGGGTTCCTG 
GTGCCTCAGG CCGAGTGGGG CAAGGCGGCC ATACTCTACT CCATGCCCCG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814881.2
CDS	305..2161
Translation	MCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDAWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSAETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_010816579.3
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816579.3

ORF Insert Sequence:

ATGTGTCATG ACATGATGGG CGGGTACCTG GATGACAAGT TTATTCAAGG CTCAGCCACA 
CAGACTCCCT ATTCCTTTTA CCACTGGCAG TACATCGACA TCTTTGTGTA CTTCAGCCAT 
CACACGGTCA CCATCCCCCC AGTAGGCTGG ACCAACGCTG CCCACAGGCA TGGGGTCTGT 
GTGCTGGGGA CTTTCATCAC TGAATGGAAA GACGGGGAGC GGCTCTGCCA GGCCTTCCTG 
GCTGGGGATG AGCACTCATA CCGGGCGGTG GCCGATCAGC TGGTCCTGAT CGCCCAGTTT 
TTCCGATTTG ACGGCTGGCT GATCAACATT GAGAACTCTC TGAGTCTGGC CGCCGTGGGG 
AACGTGCCCC ACTTCCTCCA GTACCTGACC TCTCAGCTGC ATCAGCAGGT CCCCGGGGGC 
CTGGTGCTCT GGTATGACAG CGTGGTGAGC AGCGGGCAGC TCAAATGGCA GAACGAGCTC 
AACGAGCAGA ACAGAGTCTT CTTTGATGCC TGCGACGGCT TCTTCACCAA CTATAACTGG 
CGGGAGGAGC ATCTGGAGCG GACGCTGGGG CAGGCCGGGG AGCGCCGGGC GGACGTGTAC 
GTGGGTGTAG ACGTGTTCGC CCGCGGGAAT GTCGTCGGGG GCCGGTTCGA CACGAATAAG 
TCGCTGGAGC TGATTCGGAA GCACGGGTTC TCGGCGGCCC TGTTTGCGCC TGGCTGGGTG 
TACGAGTGTT TGGAGAAGGG GAATTTCTTC CAGAACCAGG ACAAGTTCTG GAGTTTGCTG 
GAACGCTACT TGCCCACACA CAGCATCTGC TCCTTGCCCT TTGTCACTTC CTTCTGCCTG 
GGCATGGGGA CTCGAAGAGT CTACTATGGC CAGGAGGAGG CCGTGGGGCC CTGGTACCAC 
CCGAGCGCCC AGGAGATCCA GCCCCTGTTT GGAGAGCACA GGCTGGAAGG GGACGGGCAG 
GGCTGGGTGA AGACGCACTG CTGCCTGGAG GACGCCTGGA ACGGGGGCAG CTCTCTGCTC 
ATCCGAGGGC TGATTCCGCC CGAGGTTGGA AACGTGGCTG TGAGGTTATT CTCCCTGCAG 
GTCCCAGTGC CACCCAAGAT CTTCGTGTCC CTGGTGTACA AGCTGGAAGG GCCTTCGGCG 
GTGGGGGTGG CTTTGGAGCT CACCACAGGG GACGCGGGCA GCTGTCATGT GGGAGGCATC 
TCGGTGCTGA GCGCAGAAAC GAGCTCAAGG CGCAGTCCCC GACCCCTCCG GGTGCCCCCA 
ACCAAGCTGG CCAAATGGGT GGGCCGTTGC GGCCAGCAGC TCAGTGGGGG CTGGGTCCAG 
CGCTGCTACG AAGTGACTCT GCGGGGCTGC CTCCTACAGG ACCTCTTTGT TAATTTCTCC 
CGGCCTCCGG GCAGCCAGAA GGAGGAGAAC TTCATCTGTC GCCTTGGAGA GCTCCAGGTG 
GTGGATGCCA ACAGCCTGCT GACCCCGCTC CCCCAGGTGC AGGCCGTGAC GGTCTCGCAT 
GTGCGCTGGC AGCCGTTCGC CTCCGAGTGG GAGGGCGGCC CCGCCGGGCT GCGGCTCAGC 
TGTACCCTCC ACTGGGCCTA CCTCCTTCCC CGTGTCCGGT GCTTCCGGAT CCACTGCTGC 
CGAGGATCGG AAGGTGGCTC TCCCAGCAGC GGGCCTTCGC AGCCAGGGAG GCCCAGGCTG 
CTGGGCCTGG CTTTCGTCAA CCGGTATCGG ATAGTGGGCC TGGCAGTGGC AGCCGCGGAG 
CCTGGCCAGG ATGGCCGCGT GGAGTTCCTG GTGGAGCCTG TCCCCAAAGA GGGGTTCCTG 
GTGCCTCAGG CCGAGTGGGG CAAGGCGGCC ATACTCTACT CCATGCCCCG CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_005221289.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221346.2 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa10406
Clone ID Related Accession (Same CDS sequence)	XM_005221289.4
Accession Version	XM_005221289.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221289.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACGCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGCAG AAACGAGCTC AAGGCGCAGT CCCCGACCCC TCCGGGTGCC CCCAACCAAG 
CTGGCCAAAT GGGTGGGCCG TTGCGGCCAG CAGCTCAGTG GGGGCTGGGT CCAGCGCTGC 
TACGAAGTGA CTCTGCGGGG CTGCCTCCTA CAGGACCTCT TTGTTAATTT CTCCCGGCCT 
CCGGGCAGCC AGAAGGAGGA GAACTTCATC TGTCGCCTTG GAGAGCTCCA GGTGGTGGAT 
GCCAACAGCC TGCTGACCCC GCTCCCCCAG GTGCAGGCCG TGACGGTCTC GCATGTGCGC 
TGGCAGCCGT TCGCCTCCGA GTGGGAGGGC GGCCCCGCCG GGCTGCGGCT CAGCTGTACC 
CTCCACTGGG CCTACCTCCT TCCCCGTGTC CGGTGCTTCC GGATCCACTG CTGCCGAGGA 
TCGGAAGGTG GCTCTCCCAG CAGCGGGCCT TCGCAGCCAG GGAGGCCCAG GCTGCTGGGC 
CTGGCTTTCG TCAACCGGTA TCGGATAGTG GGCCTGGCAG TGGCAGCCGC GGAGCCTGGC 
CAGGATGGCC GCGTGGAGTT CCTGGTGGAG CCTGTCCCCA AAGAGGGGTT CCTGGTGCCT 
CAGGCCGAGT GGGGCAAGGC GGCCATACTC TACTCCATGC CCCGCACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221346.2
CDS	128..2338
Translation	MEEAGTGTRTAARRQGRRMRAPATLEKQRDRRPAGRLTRRRIEEDREEAVFREVVSFTPDPLPVRYYDKDTTRPISFYLSSLEELLAWRPDAEDGFNVALGPPECRQPPLSSRRPRTLMCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDAWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSAETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_005221289.4
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221289.4

ORF Insert Sequence:

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACGCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGCAG AAACGAGCTC AAGGCGCAGT CCCCGACCCC TCCGGGTGCC CCCAACCAAG 
CTGGCCAAAT GGGTGGGCCG TTGCGGCCAG CAGCTCAGTG GGGGCTGGGT CCAGCGCTGC 
TACGAAGTGA CTCTGCGGGG CTGCCTCCTA CAGGACCTCT TTGTTAATTT CTCCCGGCCT 
CCGGGCAGCC AGAAGGAGGA GAACTTCATC TGTCGCCTTG GAGAGCTCCA GGTGGTGGAT 
GCCAACAGCC TGCTGACCCC GCTCCCCCAG GTGCAGGCCG TGACGGTCTC GCATGTGCGC 
TGGCAGCCGT TCGCCTCCGA GTGGGAGGGC GGCCCCGCCG GGCTGCGGCT CAGCTGTACC 
CTCCACTGGG CCTACCTCCT TCCCCGTGTC CGGTGCTTCC GGATCCACTG CTGCCGAGGA 
TCGGAAGGTG GCTCTCCCAG CAGCGGGCCT TCGCAGCCAG GGAGGCCCAG GCTGCTGGGC 
CTGGCTTTCG TCAACCGGTA TCGGATAGTG GGCCTGGCAG TGGCAGCCGC GGAGCCTGGC 
CAGGATGGCC GCGTGGAGTT CCTGGTGGAG CCTGTCCCCA AAGAGGGGTT CCTGGTGCCT 
CAGGCCGAGT GGGGCAAGGC GGCCATACTC TACTCCATGC CCCGCACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_003587480.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587528.3 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa10407
Clone ID Related Accession (Same CDS sequence)	XM_003587480.5
Accession Version	XM_003587480.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587480.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACGCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGAAA CGAGCTCAAG GCGCAGTCCC CGACCCCTCC GGGTGCCCCC AACCAAGCTG 
GCCAAATGGG TGGGCCGTTG CGGCCAGCAG CTCAGTGGGG GCTGGGTCCA GCGCTGCTAC 
GAAGTGACTC TGCGGGGCTG CCTCCTACAG GACCTCTTTG TTAATTTCTC CCGGCCTCCG 
GGCAGCCAGA AGGAGGAGAA CTTCATCTGT CGCCTTGGAG AGCTCCAGGT GGTGGATGCC 
AACAGCCTGC TGACCCCGCT CCCCCAGGTG CAGGCCGTGA CGGTCTCGCA TGTGCGCTGG 
CAGCCGTTCG CCTCCGAGTG GGAGGGCGGC CCCGCCGGGC TGCGGCTCAG CTGTACCCTC 
CACTGGGCCT ACCTCCTTCC CCGTGTCCGG TGCTTCCGGA TCCACTGCTG CCGAGGATCG 
GAAGGTGGCT CTCCCAGCAG CGGGCCTTCG CAGCCAGGGA GGCCCAGGCT GCTGGGCCTG 
GCTTTCGTCA ACCGGTATCG GATAGTGGGC CTGGCAGTGG CAGCCGCGGA GCCTGGCCAG 
GATGGCCGCG TGGAGTTCCT GGTGGAGCCT GTCCCCAAAG AGGGGTTCCT GGTGCCTCAG 
GCCGAGTGGG GCAAGGCGGC CATACTCTAC TCCATGCCCC GCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587528.3
CDS	9..2216
Translation	MEEAGTGTRTAARRQGRRMRAPATLEKQRDRRPAGRLTRRRIEEDREEAVFREVVSFTPDPLPVRYYDKDTTRPISFYLSSLEELLAWRPDAEDGFNVALGPPECRQPPLSSRRPRTLMCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDAWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_003587480.5
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587480.5

ORF Insert Sequence:

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACGCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGAAA CGAGCTCAAG GCGCAGTCCC CGACCCCTCC GGGTGCCCCC AACCAAGCTG 
GCCAAATGGG TGGGCCGTTG CGGCCAGCAG CTCAGTGGGG GCTGGGTCCA GCGCTGCTAC 
GAAGTGACTC TGCGGGGCTG CCTCCTACAG GACCTCTTTG TTAATTTCTC CCGGCCTCCG 
GGCAGCCAGA AGGAGGAGAA CTTCATCTGT CGCCTTGGAG AGCTCCAGGT GGTGGATGCC 
AACAGCCTGC TGACCCCGCT CCCCCAGGTG CAGGCCGTGA CGGTCTCGCA TGTGCGCTGG 
CAGCCGTTCG CCTCCGAGTG GGAGGGCGGC CCCGCCGGGC TGCGGCTCAG CTGTACCCTC 
CACTGGGCCT ACCTCCTTCC CCGTGTCCGG TGCTTCCGGA TCCACTGCTG CCGAGGATCG 
GAAGGTGGCT CTCCCAGCAG CGGGCCTTCG CAGCCAGGGA GGCCCAGGCT GCTGGGCCTG 
GCTTTCGTCA ACCGGTATCG GATAGTGGGC CTGGCAGTGG CAGCCGCGGA GCCTGGCCAG 
GATGGCCGCG TGGAGTTCCT GGTGGAGCCT GTCCCCAAAG AGGGGTTCCT GGTGCCTCAG 
GCCGAGTGGG GCAAGGCGGC CATACTCTAC TCCATGCCCC GCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_024980999.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836767.1 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa10408
Clone ID Related Accession (Same CDS sequence)	XM_024980999.1
Accession Version	XM_024980999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG GGACTTTCAT CACTGAATGG AAAGACGGGG AGCGGCTCTG CCAGGCCTTC 
CTGGCTGGGG ATGAGCACTC ATACCGGGCG GTGGCCGATC AGCTGGTCCT GATCGCCCAG 
TTTTTCCGAT TTGACGGCTG GCTGATCAAC ATTGAGAACT CTCTGAGTCT GGCCGCCGTG 
GGGAACGTGC CCCACTTCCT CCAGTACCTG ACCTCTCAGC TGCATCAGCA GGTCCCCGGG 
GGCCTGGTGC TCTGGTATGA CAGCGTGGTG AGCAGCGGGC AGCTCAAATG GCAGAACGAG 
CTCAACGAGC AGAACAGAGT CTTCTTTGAT GCCTGCGACG GCTTCTTCAC CAACTATAAC 
TGGCGGGAGG AGCATCTGGA GCGGACGCTG GGGCAGGCCG GGGAGCGCCG GGCGGACGTG 
TACGTGGGTG TAGACGTGTT CGCCCGCGGG AATGTCGTCG GGGGCCGGTT CGACACGAAT 
AAGTCGCTGG AGCTGATTCG GAAGCACGGG TTCTCGGCGG CCCTGTTTGC GCCTGGCTGG 
GTGTACGAGT GTTTGGAGAA GGGGAATTTC TTCCAGAACC AGGACAAGTT CTGGAGTTTG 
CTGGAACGCT ACTTGCCCAC ACACAGCATC TGCTCCTTGC CCTTTGTCAC TTCCTTCTGC 
CTGGGCATGG GGACTCGAAG AGTCTACTAT GGCCAGGAGG AGGCCGTGGG GCCCTGGTAC 
CACCCGAGCG CCCAGGAGAT CCAGCCCCTG TTTGGAGAGC ACAGGCTGGA AGGGGACGGG 
CAGGGCTGGG TGAAGACGCA CTGCTGCCTG GAGGACGCCT GGAACGGGGG CAGCTCTCTG 
CTCATCCGAG GGCTGATTCC GCCCGAGGTT GGAAACGTGG CTGTGAGGTT ATTCTCCCTG 
CAGGTCCCAG TGCCACCCAA GATCTTCGTG TCCCTGGTGT ACAAGCTGGA AGGGCCTTCG 
GCGGTGGGGG TGGCTTTGGA GCTCACCACA GGGGACGCGG GCAGCTGTCA TGTGGGAGGC 
ATCTCGGTGC TGAGCGCAGA AACGAGCTCA AGGCGCAGTC CCCGACCCCT CCGGGTGCCC 
CCAACCAAGC TGGCCAAATG GGTGGGCCGT TGCGGCCAGC AGCTCAGTGG GGGCTGGGTC 
CAGCGCTGCT ACGAAGTGAC TCTGCGGGGC TGCCTCCTAC AGGACCTCTT TGTTAATTTC 
TCCCGGCCTC CGGGCAGCCA GAAGGAGGAG AACTTCATCT GTCGCCTTGG AGAGCTCCAG 
GTGGTGGATG CCAACAGCCT GCTGACCCCG CTCCCCCAGG TGCAGGCCGT GACGGTCTCG 
CATGTGCGCT GGCAGCCGTT CGCCTCCGAG TGGGAGGGCG GCCCCGCCGG GCTGCGGCTC 
AGCTGTACCC TCCACTGGGC CTACCTCCTT CCCCGTGTCC GGTGCTTCCG GATCCACTGC 
TGCCGAGGAT CGGAAGGTGG CTCTCCCAGC AGCGGGCCTT CGCAGCCAGG GAGGCCCAGG 
CTGCTGGGCC TGGCTTTCGT CAACCGGTAT CGGATAGTGG GCCTGGCAGT GGCAGCCGCG 
GAGCCTGGCC AGGATGGCCG CGTGGAGTTC CTGGTGGAGC CTGTCCCCAA AGAGGGGTTC 
CTGGTGCCTC AGGCCGAGTG GGGCAAGGCG GCCATACTCT ACTCCATGCC CCGCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836767.1
CDS	132..1991
Translation	MEEAGTGTRTAARRQGRRMRAPATLEKQRDRRPAGRLTRRRIEEDREEAVFREVVSFTPDPLPGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDAWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSAETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_024980999.1
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980999.1

ORF Insert Sequence:

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG GGACTTTCAT CACTGAATGG AAAGACGGGG AGCGGCTCTG CCAGGCCTTC 
CTGGCTGGGG ATGAGCACTC ATACCGGGCG GTGGCCGATC AGCTGGTCCT GATCGCCCAG 
TTTTTCCGAT TTGACGGCTG GCTGATCAAC ATTGAGAACT CTCTGAGTCT GGCCGCCGTG 
GGGAACGTGC CCCACTTCCT CCAGTACCTG ACCTCTCAGC TGCATCAGCA GGTCCCCGGG 
GGCCTGGTGC TCTGGTATGA CAGCGTGGTG AGCAGCGGGC AGCTCAAATG GCAGAACGAG 
CTCAACGAGC AGAACAGAGT CTTCTTTGAT GCCTGCGACG GCTTCTTCAC CAACTATAAC 
TGGCGGGAGG AGCATCTGGA GCGGACGCTG GGGCAGGCCG GGGAGCGCCG GGCGGACGTG 
TACGTGGGTG TAGACGTGTT CGCCCGCGGG AATGTCGTCG GGGGCCGGTT CGACACGAAT 
AAGTCGCTGG AGCTGATTCG GAAGCACGGG TTCTCGGCGG CCCTGTTTGC GCCTGGCTGG 
GTGTACGAGT GTTTGGAGAA GGGGAATTTC TTCCAGAACC AGGACAAGTT CTGGAGTTTG 
CTGGAACGCT ACTTGCCCAC ACACAGCATC TGCTCCTTGC CCTTTGTCAC TTCCTTCTGC 
CTGGGCATGG GGACTCGAAG AGTCTACTAT GGCCAGGAGG AGGCCGTGGG GCCCTGGTAC 
CACCCGAGCG CCCAGGAGAT CCAGCCCCTG TTTGGAGAGC ACAGGCTGGA AGGGGACGGG 
CAGGGCTGGG TGAAGACGCA CTGCTGCCTG GAGGACGCCT GGAACGGGGG CAGCTCTCTG 
CTCATCCGAG GGCTGATTCC GCCCGAGGTT GGAAACGTGG CTGTGAGGTT ATTCTCCCTG 
CAGGTCCCAG TGCCACCCAA GATCTTCGTG TCCCTGGTGT ACAAGCTGGA AGGGCCTTCG 
GCGGTGGGGG TGGCTTTGGA GCTCACCACA GGGGACGCGG GCAGCTGTCA TGTGGGAGGC 
ATCTCGGTGC TGAGCGCAGA AACGAGCTCA AGGCGCAGTC CCCGACCCCT CCGGGTGCCC 
CCAACCAAGC TGGCCAAATG GGTGGGCCGT TGCGGCCAGC AGCTCAGTGG GGGCTGGGTC 
CAGCGCTGCT ACGAAGTGAC TCTGCGGGGC TGCCTCCTAC AGGACCTCTT TGTTAATTTC 
TCCCGGCCTC CGGGCAGCCA GAAGGAGGAG AACTTCATCT GTCGCCTTGG AGAGCTCCAG 
GTGGTGGATG CCAACAGCCT GCTGACCCCG CTCCCCCAGG TGCAGGCCGT GACGGTCTCG 
CATGTGCGCT GGCAGCCGTT CGCCTCCGAG TGGGAGGGCG GCCCCGCCGG GCTGCGGCTC 
AGCTGTACCC TCCACTGGGC CTACCTCCTT CCCCGTGTCC GGTGCTTCCG GATCCACTGC 
TGCCGAGGAT CGGAAGGTGG CTCTCCCAGC AGCGGGCCTT CGCAGCCAGG GAGGCCCAGG 
CTGCTGGGCC TGGCTTTCGT CAACCGGTAT CGGATAGTGG GCCTGGCAGT GGCAGCCGCG 
GAGCCTGGCC AGGATGGCCG CGTGGAGTTC CTGGTGGAGC CTGTCCCCAA AGAGGGGTTC 
CTGGTGCCTC AGGCCGAGTG GGGCAAGGCG GCCATACTCT ACTCCATGCC CCGCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_005221289.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221346.1 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30442
Clone ID Related Accession (Same CDS sequence)	XM_005221289.3 , XM_005195832.3
Accession Version	XM_005221289.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACTCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGCAG AAACGAGCTC AAGGCGCAGT CCCCGACCCC TCCGGGTGCC CCCAACCAAG 
CTGGCCAAAT GGGTGGGCCG TTGCGGCCAG CAGCTCAGTG GGGGCTGGGT CCAGCGCTGC 
TACGAAGTGA CTCTGCGGGG CTGCCTCCTA CAGGACCTCT TTGTTAATTT CTCCCGGCCT 
CCGGGCAGCC AGAAGGAGGA GAACTTCATC TGTCGCCTTG GAGAGCTCCA GGTGGTGGAT 
GCCAACAGCC TGCTGACCCC GCTCCCCCAG GTGCAGGCCG TGACGGTCTC GCATGTGCGC 
TGGCAGCCGT TCGCCTCCGA GTGGGAGGGC GGCCCCGCCG GGCTGCGGCT CAGCTGTACC 
CTCCACTGGG CCTACCTCCT TCCCCGTGTC CGGTGCTTCC GGATCCACTG CTGCCGAGGA 
TCGGAAGGTG GCTCTCCCAG CAGCGGGCCT TCGCAGCCAG GGAGGCCCAG GCTGCTGGGC 
CTGGCTTTCG TCAACCGGTA TCGGATAGTG GGCCTGGCAG TGGCAGCCGC GGAGCCTGGC 
CAGGATGGCC GCGTGGAGTT CCTGGTGGAG CCTGTCCCCA AAGAGGGGTT CCTGGTGCCT 
CAGGCCGAGT GGGGCAAGGC GGCCATACTC TACTCCATGC CCCGCACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221346.1
CDS	16..2226
Translation	MEEAGTGTRTAARRQGRRMRAPATLEKQRDRRPAGRLTRRRIEEDREEAVFREVVSFTPDPLPVRYYDKDTTRPISFYLSSLEELLAWRPDAEDGFNVALGPPECRQPPLSSRRPRTLMCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDSWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSAETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_005221289.3
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221289.3

ORF Insert Sequence:

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACTCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGCAG AAACGAGCTC AAGGCGCAGT CCCCGACCCC TCCGGGTGCC CCCAACCAAG 
CTGGCCAAAT GGGTGGGCCG TTGCGGCCAG CAGCTCAGTG GGGGCTGGGT CCAGCGCTGC 
TACGAAGTGA CTCTGCGGGG CTGCCTCCTA CAGGACCTCT TTGTTAATTT CTCCCGGCCT 
CCGGGCAGCC AGAAGGAGGA GAACTTCATC TGTCGCCTTG GAGAGCTCCA GGTGGTGGAT 
GCCAACAGCC TGCTGACCCC GCTCCCCCAG GTGCAGGCCG TGACGGTCTC GCATGTGCGC 
TGGCAGCCGT TCGCCTCCGA GTGGGAGGGC GGCCCCGCCG GGCTGCGGCT CAGCTGTACC 
CTCCACTGGG CCTACCTCCT TCCCCGTGTC CGGTGCTTCC GGATCCACTG CTGCCGAGGA 
TCGGAAGGTG GCTCTCCCAG CAGCGGGCCT TCGCAGCCAG GGAGGCCCAG GCTGCTGGGC 
CTGGCTTTCG TCAACCGGTA TCGGATAGTG GGCCTGGCAG TGGCAGCCGC GGAGCCTGGC 
CAGGATGGCC GCGTGGAGTT CCTGGTGGAG CCTGTCCCCA AAGAGGGGTT CCTGGTGCCT 
CAGGCCGAGT GGGGCAAGGC GGCCATACTC TACTCCATGC CCCGCACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_005195832.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195889.2 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30442
Clone ID Related Accession (Same CDS sequence)	XM_005221289.3 , XM_005195832.3
Accession Version	XM_005195832.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACTCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGCAG AAACGAGCTC AAGGCGCAGT CCCCGACCCC TCCGGGTGCC CCCAACCAAG 
CTGGCCAAAT GGGTGGGCCG TTGCGGCCAG CAGCTCAGTG GGGGCTGGGT CCAGCGCTGC 
TACGAAGTGA CTCTGCGGGG CTGCCTCCTA CAGGACCTCT TTGTTAATTT CTCCCGGCCT 
CCGGGCAGCC AGAAGGAGGA GAACTTCATC TGTCGCCTTG GAGAGCTCCA GGTGGTGGAT 
GCCAACAGCC TGCTGACCCC GCTCCCCCAG GTGCAGGCCG TGACGGTCTC GCATGTGCGC 
TGGCAGCCGT TCGCCTCCGA GTGGGAGGGC GGCCCCGCCG GGCTGCGGCT CAGCTGTACC 
CTCCACTGGG CCTACCTCCT TCCCCGTGTC CGGTGCTTCC GGATCCACTG CTGCCGAGGA 
TCGGAAGGTG GCTCTCCCAG CAGCGGGCCT TCGCAGCCAG GGAGGCCCAG GCTGCTGGGC 
CTGGCTTTCG TCAACCGGTA TCGGATAGTG GGCCTGGCAG TGGCAGCCGC GGAGCCTGGC 
CAGGATGGCC GCGTGGAGTT CCTGGTGGAG CCTGTCCCCA AAGAGGGGTT CCTGGTGCCT 
CAGGCCGAGT GGGGCAAGGC GGCCATACTC TACTCCATGC CCCGCACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195889.2
CDS	16..2226
Translation	MEEAGTGTRTAARRQGRRMRAPATLEKQRDRRPAGRLTRRRIEEDREEAVFREVVSFTPDPLPVRYYDKDTTRPISFYLSSLEELLAWRPDAEDGFNVALGPPECRQPPLSSRRPRTLMCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDSWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSAETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_005195832.3
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195832.3

ORF Insert Sequence:

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACTCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGCAG AAACGAGCTC AAGGCGCAGT CCCCGACCCC TCCGGGTGCC CCCAACCAAG 
CTGGCCAAAT GGGTGGGCCG TTGCGGCCAG CAGCTCAGTG GGGGCTGGGT CCAGCGCTGC 
TACGAAGTGA CTCTGCGGGG CTGCCTCCTA CAGGACCTCT TTGTTAATTT CTCCCGGCCT 
CCGGGCAGCC AGAAGGAGGA GAACTTCATC TGTCGCCTTG GAGAGCTCCA GGTGGTGGAT 
GCCAACAGCC TGCTGACCCC GCTCCCCCAG GTGCAGGCCG TGACGGTCTC GCATGTGCGC 
TGGCAGCCGT TCGCCTCCGA GTGGGAGGGC GGCCCCGCCG GGCTGCGGCT CAGCTGTACC 
CTCCACTGGG CCTACCTCCT TCCCCGTGTC CGGTGCTTCC GGATCCACTG CTGCCGAGGA 
TCGGAAGGTG GCTCTCCCAG CAGCGGGCCT TCGCAGCCAG GGAGGCCCAG GCTGCTGGGC 
CTGGCTTTCG TCAACCGGTA TCGGATAGTG GGCCTGGCAG TGGCAGCCGC GGAGCCTGGC 
CAGGATGGCC GCGTGGAGTT CCTGGTGGAG CCTGTCCCCA AAGAGGGGTT CCTGGTGCCT 
CAGGCCGAGT GGGGCAAGGC GGCCATACTC TACTCCATGC CCCGCACGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_003583636.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583684.3 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30443
Clone ID Related Accession (Same CDS sequence)	XM_003583636.4 , XM_003587480.4
Accession Version	XM_003583636.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583636.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACTCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGAAA CGAGCTCAAG GCGCAGTCCC CGACCCCTCC GGGTGCCCCC AACCAAGCTG 
GCCAAATGGG TGGGCCGTTG CGGCCAGCAG CTCAGTGGGG GCTGGGTCCA GCGCTGCTAC 
GAAGTGACTC TGCGGGGCTG CCTCCTACAG GACCTCTTTG TTAATTTCTC CCGGCCTCCG 
GGCAGCCAGA AGGAGGAGAA CTTCATCTGT CGCCTTGGAG AGCTCCAGGT GGTGGATGCC 
AACAGCCTGC TGACCCCGCT CCCCCAGGTG CAGGCCGTGA CGGTCTCGCA TGTGCGCTGG 
CAGCCGTTCG CCTCCGAGTG GGAGGGCGGC CCCGCCGGGC TGCGGCTCAG CTGTACCCTC 
CACTGGGCCT ACCTCCTTCC CCGTGTCCGG TGCTTCCGGA TCCACTGCTG CCGAGGATCG 
GAAGGTGGCT CTCCCAGCAG CGGGCCTTCG CAGCCAGGGA GGCCCAGGCT GCTGGGCCTG 
GCTTTCGTCA ACCGGTATCG GATAGTGGGC CTGGCAGTGG CAGCCGCGGA GCCTGGCCAG 
GATGGCCGCG TGGAGTTCCT GGTGGAGCCT GTCCCCAAAG AGGGGTTCCT GGTGCCTCAG 
GCCGAGTGGG GCAAGGCGGC CATACTCTAC TCCATGCCCC GCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583684.3
CDS	16..2223
Translation	MEEAGTGTRTAARRQGRRMRAPATLEKQRDRRPAGRLTRRRIEEDREEAVFREVVSFTPDPLPVRYYDKDTTRPISFYLSSLEELLAWRPDAEDGFNVALGPPECRQPPLSSRRPRTLMCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDSWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_003583636.4
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583636.4

ORF Insert Sequence:

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACTCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGAAA CGAGCTCAAG GCGCAGTCCC CGACCCCTCC GGGTGCCCCC AACCAAGCTG 
GCCAAATGGG TGGGCCGTTG CGGCCAGCAG CTCAGTGGGG GCTGGGTCCA GCGCTGCTAC 
GAAGTGACTC TGCGGGGCTG CCTCCTACAG GACCTCTTTG TTAATTTCTC CCGGCCTCCG 
GGCAGCCAGA AGGAGGAGAA CTTCATCTGT CGCCTTGGAG AGCTCCAGGT GGTGGATGCC 
AACAGCCTGC TGACCCCGCT CCCCCAGGTG CAGGCCGTGA CGGTCTCGCA TGTGCGCTGG 
CAGCCGTTCG CCTCCGAGTG GGAGGGCGGC CCCGCCGGGC TGCGGCTCAG CTGTACCCTC 
CACTGGGCCT ACCTCCTTCC CCGTGTCCGG TGCTTCCGGA TCCACTGCTG CCGAGGATCG 
GAAGGTGGCT CTCCCAGCAG CGGGCCTTCG CAGCCAGGGA GGCCCAGGCT GCTGGGCCTG 
GCTTTCGTCA ACCGGTATCG GATAGTGGGC CTGGCAGTGG CAGCCGCGGA GCCTGGCCAG 
GATGGCCGCG TGGAGTTCCT GGTGGAGCCT GTCCCCAAAG AGGGGTTCCT GGTGCCTCAG 
GCCGAGTGGG GCAAGGCGGC CATACTCTAC TCCATGCCCC GCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ENGASE ( XM_003587480.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587528.2 Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30443
Clone ID Related Accession (Same CDS sequence)	XM_003583636.4 , XM_003587480.4
Accession Version	XM_003587480.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytosolic endo-beta-N-acetylglucosaminidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587480.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACTCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGAAA CGAGCTCAAG GCGCAGTCCC CGACCCCTCC GGGTGCCCCC AACCAAGCTG 
GCCAAATGGG TGGGCCGTTG CGGCCAGCAG CTCAGTGGGG GCTGGGTCCA GCGCTGCTAC 
GAAGTGACTC TGCGGGGCTG CCTCCTACAG GACCTCTTTG TTAATTTCTC CCGGCCTCCG 
GGCAGCCAGA AGGAGGAGAA CTTCATCTGT CGCCTTGGAG AGCTCCAGGT GGTGGATGCC 
AACAGCCTGC TGACCCCGCT CCCCCAGGTG CAGGCCGTGA CGGTCTCGCA TGTGCGCTGG 
CAGCCGTTCG CCTCCGAGTG GGAGGGCGGC CCCGCCGGGC TGCGGCTCAG CTGTACCCTC 
CACTGGGCCT ACCTCCTTCC CCGTGTCCGG TGCTTCCGGA TCCACTGCTG CCGAGGATCG 
GAAGGTGGCT CTCCCAGCAG CGGGCCTTCG CAGCCAGGGA GGCCCAGGCT GCTGGGCCTG 
GCTTTCGTCA ACCGGTATCG GATAGTGGGC CTGGCAGTGG CAGCCGCGGA GCCTGGCCAG 
GATGGCCGCG TGGAGTTCCT GGTGGAGCCT GTCCCCAAAG AGGGGTTCCT GGTGCCTCAG 
GCCGAGTGGG GCAAGGCGGC CATACTCTAC TCCATGCCCC GCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587528.2
CDS	16..2223
Translation	MEEAGTGTRTAARRQGRRMRAPATLEKQRDRRPAGRLTRRRIEEDREEAVFREVVSFTPDPLPVRYYDKDTTRPISFYLSSLEELLAWRPDAEDGFNVALGPPECRQPPLSSRRPRTLMCHDMMGGYLDDKFIQGSATQTPYSFYHWQYIDIFVYFSHHTVTIPPVGWTNAAHRHGVCVLGTFITEWKDGERLCQAFLAGDEHSYRAVADQLVLIAQFFRFDGWLINIENSLSLAAVGNVPHFLQYLTSQLHQQVPGGLVLWYDSVVSSGQLKWQNELNEQNRVFFDACDGFFTNYNWREEHLERTLGQAGERRADVYVGVDVFARGNVVGGRFDTNKSLELIRKHGFSAALFAPGWVYECLEKGNFFQNQDKFWSLLERYLPTHSICSLPFVTSFCLGMGTRRVYYGQEEAVGPWYHPSAQEIQPLFGEHRLEGDGQGWVKTHCCLEDSWNGGSSLLIRGLIPPEVGNVAVRLFSLQVPVPPKIFVSLVYKLEGPSAVGVALELTTGDAGSCHVGGISVLSETSSRRSPRPLRVPPTKLAKWVGRCGQQLSGGWVQRCYEVTLRGCLLQDLFVNFSRPPGSQKEENFICRLGELQVVDANSLLTPLPQVQAVTVSHVRWQPFASEWEGGPAGLRLSCTLHWAYLLPRVRCFRIHCCRGSEGGSPSSGPSQPGRPRLLGLAFVNRYRIVGLAVAAAEPGQDGRVEFLVEPVPKEGFLVPQAEWGKAAILYSMPRT

Target ORF information:

RefSeq Version	XM_003587480.4
Organism	Bos taurus(cattle)
Definition	Bos taurus endo-beta-N-acetylglucosaminidase (ENGASE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587480.4

ORF Insert Sequence:

ATGGAGGAGG CAGGGACAGG GACCCGGACG GCGGCGCGGC GGCAGGGGCG GCGGATGCGG 
GCCCCGGCGA CCTTGGAGAA GCAGCGGGAT CGGCGGCCGG CAGGGCGGCT GACCCGGAGG 
AGAATTGAAG AGGATCGAGA AGAAGCAGTC TTTCGAGAAG TGGTCAGTTT TACCCCAGAC 
CCTTTGCCAG TTAGATATTA TGACAAGGAC ACCACCAGAC CCATCAGCTT TTACTTGTCT 
TCCCTGGAGG AACTCTTGGC GTGGAGACCC GATGCGGAAG ATGGCTTTAA TGTGGCCTTG 
GGGCCCCCTG AGTGTCGGCA GCCCCCTCTG AGCAGCCGGA GGCCCAGAAC TTTGATGTGT 
CATGACATGA TGGGCGGGTA CCTGGATGAC AAGTTTATTC AAGGCTCAGC CACACAGACT 
CCCTATTCCT TTTACCACTG GCAGTACATC GACATCTTTG TGTACTTCAG CCATCACACG 
GTCACCATCC CCCCAGTAGG CTGGACCAAC GCTGCCCACA GGCATGGGGT CTGTGTGCTG 
GGGACTTTCA TCACTGAATG GAAAGACGGG GAGCGGCTCT GCCAGGCCTT CCTGGCTGGG 
GATGAGCACT CATACCGGGC GGTGGCCGAT CAGCTGGTCC TGATCGCCCA GTTTTTCCGA 
TTTGACGGCT GGCTGATCAA CATTGAGAAC TCTCTGAGTC TGGCCGCCGT GGGGAACGTG 
CCCCACTTCC TCCAGTACCT GACCTCTCAG CTGCATCAGC AGGTCCCCGG GGGCCTGGTG 
CTCTGGTATG ACAGCGTGGT GAGCAGCGGG CAGCTCAAAT GGCAGAACGA GCTCAACGAG 
CAGAACAGAG TCTTCTTTGA TGCCTGCGAC GGCTTCTTCA CCAACTATAA CTGGCGGGAG 
GAGCATCTGG AGCGGACGCT GGGGCAGGCC GGGGAGCGCC GGGCGGACGT GTACGTGGGT 
GTAGACGTGT TCGCCCGCGG GAATGTCGTC GGGGGCCGGT TCGACACGAA TAAGTCGCTG 
GAGCTGATTC GGAAGCACGG GTTCTCGGCG GCCCTGTTTG CGCCTGGCTG GGTGTACGAG 
TGTTTGGAGA AGGGGAATTT CTTCCAGAAC CAGGACAAGT TCTGGAGTTT GCTGGAACGC 
TACTTGCCCA CACACAGCAT CTGCTCCTTG CCCTTTGTCA CTTCCTTCTG CCTGGGCATG 
GGGACTCGAA GAGTCTACTA TGGCCAGGAG GAGGCCGTGG GGCCCTGGTA CCACCCGAGC 
GCCCAGGAGA TCCAGCCCCT GTTTGGAGAG CACAGGCTGG AAGGGGACGG GCAGGGCTGG 
GTGAAGACGC ACTGCTGCCT GGAGGACTCC TGGAACGGGG GCAGCTCTCT GCTCATCCGA 
GGGCTGATTC CGCCCGAGGT TGGAAACGTG GCTGTGAGGT TATTCTCCCT GCAGGTCCCA 
GTGCCACCCA AGATCTTCGT GTCCCTGGTG TACAAGCTGG AAGGGCCTTC GGCGGTGGGG 
GTGGCTTTGG AGCTCACCAC AGGGGACGCG GGCAGCTGTC ATGTGGGAGG CATCTCGGTG 
CTGAGCGAAA CGAGCTCAAG GCGCAGTCCC CGACCCCTCC GGGTGCCCCC AACCAAGCTG 
GCCAAATGGG TGGGCCGTTG CGGCCAGCAG CTCAGTGGGG GCTGGGTCCA GCGCTGCTAC 
GAAGTGACTC TGCGGGGCTG CCTCCTACAG GACCTCTTTG TTAATTTCTC CCGGCCTCCG 
GGCAGCCAGA AGGAGGAGAA CTTCATCTGT CGCCTTGGAG AGCTCCAGGT GGTGGATGCC 
AACAGCCTGC TGACCCCGCT CCCCCAGGTG CAGGCCGTGA CGGTCTCGCA TGTGCGCTGG 
CAGCCGTTCG CCTCCGAGTG GGAGGGCGGC CCCGCCGGGC TGCGGCTCAG CTGTACCCTC 
CACTGGGCCT ACCTCCTTCC CCGTGTCCGG TGCTTCCGGA TCCACTGCTG CCGAGGATCG 
GAAGGTGGCT CTCCCAGCAG CGGGCCTTCG CAGCCAGGGA GGCCCAGGCT GCTGGGCCTG 
GCTTTCGTCA ACCGGTATCG GATAGTGGGC CTGGCAGTGG CAGCCGCGGA GCCTGGCCAG 
GATGGCCGCG TGGAGTTCCT GGTGGAGCCT GTCCCCAAAG AGGGGTTCCT GGTGCCTCAG 
GCCGAGTGGG GCAAGGCGGC CATACTCTAC TCCATGCCCC GCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ENGASE
Entrez Gene ID	100847654
Full Name	endo-beta-N-acetylglucosaminidase
General protein information	Preferred Names endo-beta-N-acetylglucosaminidase Names cytosolic endo-beta-N-acetylglucosaminidase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	ENGASE	XP_001159278.1
Canis lupus familiaris (dog)	ENGASE	XP_005624210.1
Rattus norvegicus (Norway rat)	Engase	NP_001100540.1
Danio rerio (zebrafish)	engase	NP_001074047.2
Homo sapiens (human)	ENGASE	NP_001036038.1
Gallus gallus (chicken)	ENGASE	XP_427660.4
Arabidopsis thaliana (thale cress)	AT3G11040	NP_187715.1
Oryza sativa (rice)	Os05g0346500	NP_001055263.2
Xenopus tropicalis (tropical clawed frog)	engase	XP_002932564.2
Arabidopsis thaliana (thale cress)	AT5G05460	NP_196165.3
Mus musculus (house mouse)	Engase	NP_766161.1
Bos taurus (cattle)	ENGASE	XP_005221346.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ENGASE cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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