SYMPK cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SYMPK
Entrez Gene ID	100337373
Full Name	symplekin
General protein information	Preferred Names symplekin Names symplekin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005219143.3	XP_005219200.1	symplekin isoform X1
XM_005219142.3	XP_005219199.1	symplekin isoform X1
NM_001206913.1	NP_001193842.1	symplekin
XM_010814988.2	XP_010813290.1	symplekin isoform X1
XM_010814987.2	XP_010813289.1	symplekin isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SYMPK	NP_001193842.1
Danio rerio (zebrafish)	si:dkeyp-117b8.1	XP_005165630.1
Homo sapiens (human)	SYMPK	NP_004810.2
Caenorhabditis elegans (roundworm)	symk-1	NP_505210.2
Mus musculus (house mouse)	Sympk	NP_080881.2
Rattus norvegicus (Norway rat)	Sympk	NP_001094300.1
Canis lupus familiaris (dog)	SYMPK	XP_533638.2
Drosophila melanogaster (fruit fly)	Sym	NP_649580.1
Xenopus tropicalis (tropical clawed frog)	sympk	NP_989259.2
Pan troglodytes (chimpanzee)	SYMPK	XP_001166898.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002618	XP_312315.5

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SYMPK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SYMPK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa28797	XM_005219143.3 Latest version!	Bos taurus symplekin (SYMPK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OBa28797	XM_010814987.2 Latest version!	Bos taurus symplekin (SYMPK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OBa28797	XM_010814988.2 Latest version!	Bos taurus symplekin (SYMPK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OBa28797	XM_005219142.3 Latest version!	Bos taurus symplekin (SYMPK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OBa01251	NM_001206913.1 Latest version!	Bos taurus symplekin (SYMPK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SYMPK ( XM_005219143.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219200.1 Bos taurus symplekin (SYMPK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa28797
Clone ID Related Accession (Same CDS sequence)	XM_005219143.3 , XM_005219142.3 , XM_010814988.2 , XM_010814987.2
Accession Version	XM_005219143.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	symplekin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCTTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCACCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCAAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CTGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219200.1
CDS	211..4029
Translation	MAASSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEVPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLNVLKHPASLEFQAQITTLLVDLGTPQVEIARNMPSGKDARKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKPESVLIKRRLPAQGQAISVVGSLSSMSSLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFDSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGVKGALDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVIRKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKLIKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSAKCDMKSIIKATNLCFAERNVYTSEVLAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIKCCQRTKPQSFQVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMAILEASGKQEPETKEAPAGPLEEDDLEPLALAPPPAPRPPQDLIGLRLAQEKALKRQLEEEQKLKPGGLGGPSSSSSSSSARPGPPQPEEAIDFREEGPECETPAIFISMDDDSGLTEAALLDSSLEGPLPKETAAGGLLSKEERSPQPLTAAGEDTVQTPSPATEEVGEPETKGNS

Target ORF information:

RefSeq Version	XM_005219143.3
Organism	Bos taurus(cattle)
Definition	Bos taurus symplekin (SYMPK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219143.3

ORF Insert Sequence:

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCTTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCACCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCAAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CTGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SYMPK ( XM_005219142.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219199.1 Bos taurus symplekin (SYMPK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28797
Clone ID Related Accession (Same CDS sequence)	XM_005219143.3 , XM_005219142.3 , XM_010814988.2 , XM_010814987.2
Accession Version	XM_005219142.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	symplekin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219142.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCTTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCACCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCAAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CTGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219199.1
CDS	368..4186
Translation	MAASSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEVPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLNVLKHPASLEFQAQITTLLVDLGTPQVEIARNMPSGKDARKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKPESVLIKRRLPAQGQAISVVGSLSSMSSLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFDSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGVKGALDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVIRKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKLIKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSAKCDMKSIIKATNLCFAERNVYTSEVLAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIKCCQRTKPQSFQVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMAILEASGKQEPETKEAPAGPLEEDDLEPLALAPPPAPRPPQDLIGLRLAQEKALKRQLEEEQKLKPGGLGGPSSSSSSSSARPGPPQPEEAIDFREEGPECETPAIFISMDDDSGLTEAALLDSSLEGPLPKETAAGGLLSKEERSPQPLTAAGEDTVQTPSPATEEVGEPETKGNS

Target ORF information:

RefSeq Version	XM_005219142.3
Organism	Bos taurus(cattle)
Definition	Bos taurus symplekin (SYMPK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219142.3

ORF Insert Sequence:

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCTTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCACCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCAAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CTGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SYMPK ( XM_010814988.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813290.1 Bos taurus symplekin (SYMPK), transcript variant X3, mRNA.

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CloneID	OBa28797
Clone ID Related Accession (Same CDS sequence)	XM_005219143.3 , XM_005219142.3 , XM_010814988.2 , XM_010814987.2
Accession Version	XM_010814988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	symplekin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCTTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCACCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCAAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CTGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813290.1
CDS	366..4184
Translation	MAASSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEVPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLNVLKHPASLEFQAQITTLLVDLGTPQVEIARNMPSGKDARKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKPESVLIKRRLPAQGQAISVVGSLSSMSSLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFDSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGVKGALDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVIRKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKLIKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSAKCDMKSIIKATNLCFAERNVYTSEVLAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIKCCQRTKPQSFQVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMAILEASGKQEPETKEAPAGPLEEDDLEPLALAPPPAPRPPQDLIGLRLAQEKALKRQLEEEQKLKPGGLGGPSSSSSSSSARPGPPQPEEAIDFREEGPECETPAIFISMDDDSGLTEAALLDSSLEGPLPKETAAGGLLSKEERSPQPLTAAGEDTVQTPSPATEEVGEPETKGNS

Target ORF information:

RefSeq Version	XM_010814988.2
Organism	Bos taurus(cattle)
Definition	Bos taurus symplekin (SYMPK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814988.2

ORF Insert Sequence:

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCTTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCACCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCAAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CTGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SYMPK ( XM_010814987.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813289.1 Bos taurus symplekin (SYMPK), transcript variant X2, mRNA.

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CloneID	OBa28797
Clone ID Related Accession (Same CDS sequence)	XM_005219143.3 , XM_005219142.3 , XM_010814988.2 , XM_010814987.2
Accession Version	XM_010814987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	symplekin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCTTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCACCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCAAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CTGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813289.1
CDS	290..4108
Translation	MAASSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEVPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLNVLKHPASLEFQAQITTLLVDLGTPQVEIARNMPSGKDARKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKPESVLIKRRLPAQGQAISVVGSLSSMSSLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFDSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGVKGALDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVIRKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKLIKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSAKCDMKSIIKATNLCFAERNVYTSEVLAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIKCCQRTKPQSFQVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMAILEASGKQEPETKEAPAGPLEEDDLEPLALAPPPAPRPPQDLIGLRLAQEKALKRQLEEEQKLKPGGLGGPSSSSSSSSARPGPPQPEEAIDFREEGPECETPAIFISMDDDSGLTEAALLDSSLEGPLPKETAAGGLLSKEERSPQPLTAAGEDTVQTPSPATEEVGEPETKGNS

Target ORF information:

RefSeq Version	XM_010814987.2
Organism	Bos taurus(cattle)
Definition	Bos taurus symplekin (SYMPK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814987.2

ORF Insert Sequence:

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCTTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCACCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCAAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CTGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SYMPK ( NM_001206913.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193842.1 Bos taurus symplekin (SYMPK), mRNA.

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CloneID	OBa01251
Clone ID Related Accession (Same CDS sequence)	NM_001206913.1
Accession Version	NM_001206913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	symplekin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03117243.1. On or before May 12, 2011 this sequence version replaced XM_002701895.1, XM_002686082.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCCTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCGCCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCCAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CGGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193842.1
CDS	283..4101
Translation	MAASSGDSVTRRSVASQFFTQEEGPGIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLLDNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEVPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLNVLKHPASLEFQAQITTLLVDLGTPQVEIARNMPSGKDARKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKPESVLIKRRLPAQGQAISVVGSLSSMSSLEEEAPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEAMKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFDSGLKAEVLSFILEDVRARLDLAFAWLYQEYNAYLAAGVKGALDKYEDCLIRLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVIRKYCEDESRTYLGMSTLRDLIFKRPSRQFQYLHVLLDLSSHEKDKVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLFGADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPELLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKLIKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSAKCDMKSIIKATNLCFAERNVYTSEVLAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIMKQVWKYPKVWEGFIKCCQRTKPQSFQVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMAILEASGKQEPETKEAPAGPLEEDDLEPLALAPPPAPRPPQDLIGLRLAQEKALKRQLEEEQKLKPGGLGGPSSSSSSSSARPGPPQPEEAIDFREEGPECETPAIFISMDDDSGLTEAALLDSSLEGPLPKETAAGGLLSKEERSPQPLTAAGEDTVQTPSPATEEVGEPETKGNS

Target ORF information:

RefSeq Version	NM_001206913.1
Organism	Bos taurus(cattle)
Definition	Bos taurus symplekin (SYMPK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206913.1

ORF Insert Sequence:

ATGGCAGCCA GCAGTGGGGA CAGCGTCACA CGCCGGAGCG TGGCATCACA GTTCTTCACT 
CAAGAGGAGG GGCCGGGCAT CGATGGCATG ACCACATCAG AGAGAGTGGT AGATCTCTTG 
AACCAGGCAG CACTGATCAC CAACGACTCA AAGATCACAG TGCTCAAACA GGTGCAGGAA 
CTGATCATCA ACAAAGATCC CACACTACTG GACAACTTCC TGGATGAGAT CATCGCTTTC 
CAGGCAGACA AGTCCATCGA AGTGCGCAAA TTTGTCATCG GCTTCATCGA GGAGGCTTGC 
AAGCGAGACA TTGAGCTGCT GCTGAAACTG ATCGCGAACC TCAACATGCT CTTGAGAGAC 
GAGAATGTGA ATGTGGTGAA GAAGGCCATC CTCACCATGA CCCAGCTCTA CAAGGTGGCC 
CTGCAGTGGA TGGTGAAGTC ACGGGTGATC AGTGAGCTCC AGGAGGCCTG CTGGGATATG 
GTGTCGGCCA TGGCTGGGGA CATCATCCTG CTGCTGGACT CTGACAATGA CGGCATCCGT 
ACCCATGCCA TCAAGTTCGT GGAGGGCCTC ATCGTCACCC TTTCGCCCCG CATGGCTGAC 
TCGGAGGTGC CCCGACGCCA GGAGCACGAC ATCAGCCTGG ACCGCATCCC TCGTGACCAC 
CCCTACATCC AGTACAATGT GCTGTGGGAA GAAGGCAAGG CAGCCTTGGA GCAGCTGCTC 
AAGTTCATGG TGCACCCCGC TATCTCCTCC ATCAACCTGA CCACAGCCCT GGGCTCCCTG 
GCCAATATTG CCCGCCAGAG GCCCATGTTC ATGTCAGAGG TGATTCAGGC CTACGAAACC 
CTCCACGCCA ATCTGCCCCC AACGCTGGCC AAATCTCAGG TGAGCAGCGT GCGTAAGAAT 
CTCAAGTTGC ACCTGTTGAA TGTGCTAAAG CACCCAGCGT CCTTGGAGTT CCAGGCTCAG 
ATCACCACCC TCCTGGTGGA CCTGGGCACG CCTCAGGTGG AGATCGCCCG AAACATGCCC 
AGTGGCAAGG ATGCCCGCAA GCGGCCCCGA GACGACTCGG ATTCCACACT CAAGAAGATG 
AAGCTGGAGC CCAACCTGGG GGAAGACGAT GAGGACAAGG ACTTGGAGCC AGGCCCCTCG 
GGAACCTCGA AGGCCTCAGC CCAGATCTCA GGCCAGTCAG ACACAGACAT CACAGCCGAA 
TTCCTCCAGC CTCTGCTGAC ACCCGACAAC GTGGCCAATC TGGTCCTCAT CAGCATGGTG 
TACCTGCCCG AGGCCATGCC CGCCTCCTTC CAAGCGATCT ACACCCCAGT GGAGTCAGCA 
GGCACCGAAG CCCAGATCAA GCACCTGGCT CGGCTCATGG CCACGCAGAT GACGGCCGCA 
GGACTGGGGC CAGGCGTGGA GCAGACCAAG CAGTGCAAGG AGGAGCCCAA GGAGGAGAAG 
GTGGTGAAGC CAGAGAGCGT TCTGATCAAG CGGCGCCTGC CAGCCCAGGG CCAGGCCATC 
TCGGTAGTGG GCTCCCTGAG CTCCATGTCC TCCCTGGAGG AGGAGGCGCC CCAGGCCAAG 
AGGAGGCCGG AGCCCATTAT CCCCGTCACG CAGCCCCGGC TGGCGGGTGC TGGCGGGCGC 
AAGAAAATCT TCCGCCTAAG TGACGTGCTG AAGCCCCTGA CGGACGCGCA GGTTGAGGCC 
ATGAAGCTGG GCGCTGTGAA GCGGATCCTG CGGGCCGAGA AGGCCGTGGC CTGCAGCGGG 
GCCGCCCAGG TGCGCATAAA GATCCTGGCC AGCCTGGTGA CGCAGTTCGA CTCGGGCCTG 
AAGGCCGAGG TCCTGTCCTT CATCCTGGAG GACGTCCGGG CCCGCCTGGA CCTGGCCTTC 
GCCTGGCTCT ACCAGGAGTA CAACGCCTAC CTCGCAGCTG GCGTCAAGGG CGCCCTGGAC 
AAGTATGAGG ACTGCCTCAT CCGCCTGCTC TCCGGCCTGC AGGAGAAGCC GGACCAGAAG 
GACGGGATCT TCACCAAGGT TGTGCTGGAG GCGCCGCTGA TCACCGAGAG CGCCCTGGAG 
GTGATCCGCA AGTATTGCGA GGACGAGAGT CGCACCTACC TGGGCATGTC CACTCTCCGA 
GACCTGATCT TCAAGCGCCC ATCCCGCCAG TTCCAGTACC TGCATGTCCT GCTAGACCTC 
AGCTCCCACG AGAAGGACAA GGTGCGTTCC CAGGCCTTGC TGTTCATCAA GCGCATGTAC 
GAAAAGGAGC AGCTGCGAGA GTACGTGGAG AAGTTTGCCC TCAACTACCT GCAGCTCTTG 
GTCCACCCCA ACCCGCCATC GGTGCTGTTT GGAGCTGACA AGGACACAGA GGTGGCCGCA 
CCCTGGACCG AGGAGACGGT GAAGCAGTGC CTGTACCTCT ACCTGGCTCT CCTACCTCAG 
AACCACAAGC TGATCCACGA GCTGGCGGCC GTGTACACGG AGGCCATCGC TGACATCAAG 
AGGACAGTGC TGAGGGTCAT CGAGCAGCCG ATCCGGGGGA TGGGCATGAA CTCGCCAGAG 
CTGCTCCTGC TGGTGGAGAA CTGTCCCAAG GGGGCGGAGA CACTGGTCAC CCGTTGTCTG 
CACAGCCTCA CGGACAAAGT GCCCCCGTCC CCAGAGCTGG TGAAGCGGGT CAGGGATCTC 
TACCACAAGC GACTGCCAGA CGTCCGCTTC CTCATCCCAG TGCTCAATGG GCTGGAGAAG 
AAGGAAGTGA TCCAGGCCCT GCCAAAGCTC ATCAAGCTTA ACCCCATCGT GGTGAAAGAG 
GTCTTCAATC GCCTGCTGGG CACCCAGCAC GGTGAAGGGA ACTCAGCCCT GTCCCCCCTG 
AACCCTGGAG AGCTGCTCAT CGCCTTACAC AACATTGACT CGGCAAAGTG TGACATGAAG 
TCCATCATCA AAGCCACCAA CCTGTGCTTT GCGGAGCGGA ACGTGTACAC GTCGGAGGTG 
CTGGCCGTGG TGATGCAGCA ACTGATGGAG CAGAGCCCCC TGCCCATGCT GCTCATGAGG 
ACCGTCATCC AGTCCTTGAC TATGTACCCC CGCCTGGGGG GCTTCGTCAT GAACATCCTG 
TCCCGGCTCA TCATGAAGCA GGTGTGGAAG TATCCCAAGG TGTGGGAGGG CTTCATCAAG 
TGCTGTCAGC GCACCAAGCC TCAGAGCTTC CAGGTCATCC TGCAGCTCCC ACCGCAGCAG 
CTGGGGGCCG TCTTTGACAA GTGCCCAGAG CTCCGGGAGC CCCTGCTGGC CCACGTCCGC 
TCCTTCACCC CCCACCAGCA AGCGCACATC CCCAACTCCA TCATGGCCAT CCTGGAGGCC 
AGCGGCAAGC AGGAGCCAGA GACTAAGGAA GCACCTGCCG GGCCCCTGGA AGAGGATGAC 
TTAGAGCCTC TGGCCCTGGC ACCACCTCCG GCCCCCCGAC CCCCTCAAGA TCTCATCGGC 
CTGCGGCTGG CCCAGGAGAA GGCCTTAAAG CGGCAACTGG AGGAAGAACA GAAGCTGAAG 
CCTGGGGGAC TGGGAGGCCC CTCCTCTTCC TCGTCCTCTT CCTCGGCGCG GCCAGGCCCC 
CCCCAGCCAG AGGAAGCCAT TGATTTCCGG GAGGAGGGGC CTGAGTGTGA GACCCCGGCC 
ATCTTCATCA GCATGGACGA TGACTCGGGG CTGACTGAGG CCGCACTGCT GGACTCTAGT 
CTTGAGGGAC CCCTACCTAA GGAAACTGCA GCAGGTGGAT TGCTTTCAAA GGAGGAGCGG 
AGCCCCCAGC CCCTCACAGC TGCTGGAGAA GACACAGTGC AGACTCCCAG CCCCGCCACT 
GAGGAAGTGG GCGAACCGGA GACCAAGGGG AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SYMPK cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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