TSPEAR cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSPEAR
Entrez Gene ID	100335468
Full Name	thrombospondin type laminin G domain and EAR repeats
General protein information	Preferred Names thrombospondin type laminin G domain and EAR repeats Names thrombospondin-type laminin G domain and EAR repeat-containing protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206810.1	NP_001193739.1	thrombospondin-type laminin G domain and EAR repeat-containing protein precursor
XM_025001316.1	XP_024857084.1	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSPEAR	XP_531582.2
Danio rerio (zebrafish)	tspearb	XP_682770.2
Danio rerio (zebrafish)	tspeara	XP_698991.2
Homo sapiens (human)	TSPEAR	NP_659428.2
Canis lupus familiaris (dog)	LOC611461	XP_005639060.1
Mus musculus (house mouse)	Tspear	NP_001274003.1
Gallus gallus (chicken)	TSPEAR	XP_422647.3
Bos taurus (cattle)	TSPEAR	NP_001193739.1
Xenopus tropicalis (tropical clawed frog)	tspear	XP_002936569.2

Gene Ontology
Bibliography

Related articles in PubMed

Defect in the gene encoding the EAR/EPTP domain-containing protein TSPEAR causes DFNB98 profound deafness.
Delmaghani S, Aghaie A, Michalski N, Bonnet C, Weil D, Petit C
Human molecular genetics21(17)3835-44(2012 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TSPEAR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSPEAR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01570	NM_001206810.1 Latest version!	Bos taurus thrombospondin-type laminin G domain and EAR repeats (TSPEAR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa218587	XM_025001316.1 Latest version!	Bos taurus thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSPEAR ( NM_001206810.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193739.1 Bos taurus thrombospondin-type laminin G domain and EAR repeats (TSPEAR), mRNA.

Price & Availability↑

CloneID	OBa01570
Clone ID Related Accession (Same CDS sequence)	NM_001206810.1
Accession Version	NM_001206810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	thrombospondin-type laminin G domain and EAR repeat-containing protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03091878.1, AAFC03018731.1, AAFC03031405.1 and AAFC03031404.1. On or before May 11, 2011 this sequence version replaced XM_002702332.1, XM_002685139.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822097, SAMN03145425 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCCC TGCTGACTCT GTGTGTCGTG CTCCTGCCCC TGGCGACCCC AGGCCGCGGG 
GCCCGGCAAT GGGAGCCCTG CACTGACCTG CGGCCCCTGG ATGTGCTGGC AGAGGCGGTC 
CCTCCCGATG GCTCTGCAGT CGGGGTCAGG GTGATGCAAG TCCAAGGTGC ACGAGGACTC 
CAGCTCTCAG CCGCCACGCC CCGTGCCCTG AGCTTCCCTG CCTCGAGGAT TTTCTTCAGA 
TGTGATCTCT TCCCGGAGGA GTTTTCCATC GTTGTGACCT TGAAAGTCCC CAGCCTCCCC 
CCTAAGAAGA ACGAGTACCT GCTGGCGGTG GTGGCAGAGG AGCGGGACTC GCTGCTGCTG 
GGCCTGCGAT ACTCGCCCAC CCAGCTGCAC TTCCTCTTCC TCAGCGAGGA CGGGGCAGGC 
GCCTGGCAGA CCCGCGTGTC CTTCCGCAGC CCGGCTCTGG TGGACGGCCA GTGGCACGTG 
CTGGTCTTGG CCGTGTCCGA GGGCTCCTTT TCGCTCACTA CGGACTGCGG CCCCGCCGTG 
GACATAATGG CTGACATGCC CTTTCCAGCC ACCCTATCTG TGCGAGGAGC CCGGTTCTTC 
ATCGGCAGTC GGAGGAGGAC GAAAGGCCGA TTCACGGGCC TGGTGAGGCA GCTGGTTCTG 
TTGCCTGGCT CAGATGCCAC CCCGAGGCTC TGTCCCTGCG TGAATCCTGA GCTCGCTGTG 
CTGTCCATCC CTGCTATCCT GCATGGCCTC ACAGGGAAGC CGGAAGACAA CGAGGTGCTA 
AAATACCCCT ATGAAACCAA CATGAAGGTG ACGCTGGGAC CCCGGCCTCC GTGCACCAAA 
GCGGAGGATG CCCAGTTCTG GTTCGACGCC AGCCGCAAGG GGCTATACCT GTGCGTGGGC 
AGCGAGTGGG TCTCCGTGTT AGCAGCCAAA GAAAAGCTGG ATTACGTGGA GGAGCATCAG 
AGCCTATTCA CGAACTCAGA GACGCTGGGC ATCGAGGTCT TCGTCATCCC TGAGGCTGGG 
CTCTTTGTAG CAACCGCCAA CCGGAAAACC ACATCTGCCA TCTACAAGTG GACCGATGGC 
AAGTTTGCTT CATACCAGAA CATTCCCACG CACCAGGCAC AGTCCTGGAG ACACTTCACC 
ATTGGGAAAA AGATCTTCCT GGCTGTGGCT AATTTTGAGC CGAATGAGAA GGGTCAGGAG 
TTCTCGGTCA TTTACAAGTG GAGCCAGAGA AGGCTGAGGT TCACGCCATA CCAGAGGGTC 
CCCACCCACA GTGCCCGGGA CTGGGAGGCC TTCGAGGTGG CCGGCGAGCA CTTCCTGGCC 
GTGGCCAACC ACCGGGAAGG CGACAACCAT AACATTGACA GTGTCATCTA CAAGTGGAAC 
CCAGGGACCC GGCTCTTCGA GGCCAACCAG ACCATCGCCA CTTCGGGCGC CTACGACTGG 
GAGTTCTTCA CGGTGGGGCC CTACGCCTTT CTGGCCGTAG CCAACGCCTT CAACGGCACA 
TCCACGCGGC TGCAGTCCCA CCTGTACGTG CGGCTGGACG GCTCCTTCCA GCTCTTCCAG 
TCTTTCCTGA CGTTTGGCGC CGCAGACTGG GAGGTTTTCC ACATTGGGGA GAGGGTCTTC 
CTCGCCGTGG CGAACAGTCA CCGCTACGAC GTGGAGATGC GAGTGCAGAA CGACTCCTAC 
GTCATCAACT CGGTCATATA CGAGCTGAAC GTCACCGCGC AGACGTTCGT CCGCTTCCAG 
GAGATCCGCA CCTGCAGTGC TCTGGACTGG GAGTTCTTCT CGGTGGGAGA AGATTATTTC 
CTGGTGGTTG CAAACTCCTT CGATGGAAAT ACCTTCTCGG TAAACAGTAT TATTTACAGG 
TGGCAGGGGT ATGAAGGCTT CGTGGCCGTG CACAGCCTCC CAACCTTCGG CTGCAGGGAC 
TGGGAGGCCT TCCGCACAGC GGCCGGCTCC TTCCTGGTCT ACTCCAGCGC CAAGGAACCG 
CTGTCCCGGG TGCTGAAGCT GAGGACGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193739.1
CDS	60..2072
Translation	MSALLTLCVVLLPLATPGRGARQWEPCTDLRPLDVLAEAVPPDGSAVGVRVMQVQGARGLQLSAATPRALSFPASRIFFRCDLFPEEFSIVVTLKVPSLPPKKNEYLLAVVAEERDSLLLGLRYSPTQLHFLFLSEDGAGAWQTRVSFRSPALVDGQWHVLVLAVSEGSFSLTTDCGPAVDIMADMPFPATLSVRGARFFIGSRRRTKGRFTGLVRQLVLLPGSDATPRLCPCVNPELAVLSIPAILHGLTGKPEDNEVLKYPYETNMKVTLGPRPPCTKAEDAQFWFDASRKGLYLCVGSEWVSVLAAKEKLDYVEEHQSLFTNSETLGIEVFVIPEAGLFVATANRKTTSAIYKWTDGKFASYQNIPTHQAQSWRHFTIGKKIFLAVANFEPNEKGQEFSVIYKWSQRRLRFTPYQRVPTHSARDWEAFEVAGEHFLAVANHREGDNHNIDSVIYKWNPGTRLFEANQTIATSGAYDWEFFTVGPYAFLAVANAFNGTSTRLQSHLYVRLDGSFQLFQSFLTFGAADWEVFHIGERVFLAVANSHRYDVEMRVQNDSYVINSVIYELNVTAQTFVRFQEIRTCSALDWEFFSVGEDYFLVVANSFDGNTFSVNSIIYRWQGYEGFVAVHSLPTFGCRDWEAFRTAAGSFLVYSSAKEPLSRVLKLRTG

Target ORF information:

RefSeq Version	NM_001206810.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thrombospondin-type laminin G domain and EAR repeats (TSPEAR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206810.1

ORF Insert Sequence:

ATGTCTGCCC TGCTGACTCT GTGTGTCGTG CTCCTGCCCC TGGCGACCCC AGGCCGCGGG 
GCCCGGCAAT GGGAGCCCTG CACTGACCTG CGGCCCCTGG ATGTGCTGGC AGAGGCGGTC 
CCTCCCGATG GCTCTGCAGT CGGGGTCAGG GTGATGCAAG TCCAAGGTGC ACGAGGACTC 
CAGCTCTCAG CCGCCACGCC CCGTGCCCTG AGCTTCCCTG CCTCGAGGAT TTTCTTCAGA 
TGTGATCTCT TCCCGGAGGA GTTTTCCATC GTTGTGACCT TGAAAGTCCC CAGCCTCCCC 
CCTAAGAAGA ACGAGTACCT GCTGGCGGTG GTGGCAGAGG AGCGGGACTC GCTGCTGCTG 
GGCCTGCGAT ACTCGCCCAC CCAGCTGCAC TTCCTCTTCC TCAGCGAGGA CGGGGCAGGC 
GCCTGGCAGA CCCGCGTGTC CTTCCGCAGC CCGGCTCTGG TGGACGGCCA GTGGCACGTG 
CTGGTCTTGG CCGTGTCCGA GGGCTCCTTT TCGCTCACTA CGGACTGCGG CCCCGCCGTG 
GACATAATGG CTGACATGCC CTTTCCAGCC ACCCTATCTG TGCGAGGAGC CCGGTTCTTC 
ATCGGCAGTC GGAGGAGGAC GAAAGGCCGA TTCACGGGCC TGGTGAGGCA GCTGGTTCTG 
TTGCCTGGCT CAGATGCCAC CCCGAGGCTC TGTCCCTGCG TGAATCCTGA GCTCGCTGTG 
CTGTCCATCC CTGCTATCCT GCATGGCCTC ACAGGGAAGC CGGAAGACAA CGAGGTGCTA 
AAATACCCCT ATGAAACCAA CATGAAGGTG ACGCTGGGAC CCCGGCCTCC GTGCACCAAA 
GCGGAGGATG CCCAGTTCTG GTTCGACGCC AGCCGCAAGG GGCTATACCT GTGCGTGGGC 
AGCGAGTGGG TCTCCGTGTT AGCAGCCAAA GAAAAGCTGG ATTACGTGGA GGAGCATCAG 
AGCCTATTCA CGAACTCAGA GACGCTGGGC ATCGAGGTCT TCGTCATCCC TGAGGCTGGG 
CTCTTTGTAG CAACCGCCAA CCGGAAAACC ACATCTGCCA TCTACAAGTG GACCGATGGC 
AAGTTTGCTT CATACCAGAA CATTCCCACG CACCAGGCAC AGTCCTGGAG ACACTTCACC 
ATTGGGAAAA AGATCTTCCT GGCTGTGGCT AATTTTGAGC CGAATGAGAA GGGTCAGGAG 
TTCTCGGTCA TTTACAAGTG GAGCCAGAGA AGGCTGAGGT TCACGCCATA CCAGAGGGTC 
CCCACCCACA GTGCCCGGGA CTGGGAGGCC TTCGAGGTGG CCGGCGAGCA CTTCCTGGCC 
GTGGCCAACC ACCGGGAAGG CGACAACCAT AACATTGACA GTGTCATCTA CAAGTGGAAC 
CCAGGGACCC GGCTCTTCGA GGCCAACCAG ACCATCGCCA CTTCGGGCGC CTACGACTGG 
GAGTTCTTCA CGGTGGGGCC CTACGCCTTT CTGGCCGTAG CCAACGCCTT CAACGGCACA 
TCCACGCGGC TGCAGTCCCA CCTGTACGTG CGGCTGGACG GCTCCTTCCA GCTCTTCCAG 
TCTTTCCTGA CGTTTGGCGC CGCAGACTGG GAGGTTTTCC ACATTGGGGA GAGGGTCTTC 
CTCGCCGTGG CGAACAGTCA CCGCTACGAC GTGGAGATGC GAGTGCAGAA CGACTCCTAC 
GTCATCAACT CGGTCATATA CGAGCTGAAC GTCACCGCGC AGACGTTCGT CCGCTTCCAG 
GAGATCCGCA CCTGCAGTGC TCTGGACTGG GAGTTCTTCT CGGTGGGAGA AGATTATTTC 
CTGGTGGTTG CAAACTCCTT CGATGGAAAT ACCTTCTCGG TAAACAGTAT TATTTACAGG 
TGGCAGGGGT ATGAAGGCTT CGTGGCCGTG CACAGCCTCC CAACCTTCGG CTGCAGGGAC 
TGGGAGGCCT TCCGCACAGC GGCCGGCTCC TTCCTGGTCT ACTCCAGCGC CAAGGAACCG 
CTGTCCCGGG TGCTGAAGCT GAGGACGGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSPEAR ( XM_025001316.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857084.1 Bos taurus thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa218587
Clone ID Related Accession (Same CDS sequence)	XM_025001316.1
Accession Version	XM_025001316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	thrombospondin-type laminin G domain and EAR repeat-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTCC AAGGTGCACG AGGACTCCAG CTCTCAGCCG CCACGCCCCG TGCCCTGAGC 
TTCCCTGCCT CGAGGATTTT CTTCAGATGT GATCTCTTCC CGGAGGAATT TTCCATCGTT 
GTGACCTTGA AAGTCCCCAG CCTCCCCCCT AAGAAGAACG AGTACCTGCT GGCGGTGGTG 
GCAGAGGAGC GGGACTCGCT GCTGCTGGGC CTGCGATACT CGCCCACCCA GCTGCACTTC 
CTCTTCCTCA GCGAGGACGG GGCAGGCGCC TGGCAGACCC GCGTGTCCTT CCGCAGCCCG 
GCTCTGGTGG ACGGCCAGTG GCACGTGCTG GTCTTGGCCG TGTCCGAGGG CTCCTTTTCG 
CTCACTACGG ACTGCGGCCC CGCCGTGGAC ATAATGGCTG ACATGCCCTT TCCAGCCACC 
CTATCTGTGC GAGGAGCCCG GTTCTTCATC GGCAGTCGGA GGAGGACGAA AGGCCGATTC 
ACGGGCCTGG TGAGGCAGCT GGTTCTGTTG CCTGGCTCAG ATGCCACCCC GAGGCTCTGT 
CCCTGCGTGA ATCCTGAGCT CGCTGTGCTG TCCATCCCTG CTATCCTGCA TGGCCTCACA 
GGGAAGCCGG AAGACAACGA GGTGCTAAAA TACCCCTATG AAACCAACAT GAAGGTGACG 
CTGGGACCCC GGCCTCCGTG CACCAAAGCG GAGGATGCCC AGTTCTGGTT CGACGCCAGC 
CGCAAGGGGC TATACCTGTG CGTGGGCAGC GAGTGGGTCT CCGTGTTAGC AGCCAAAGAA 
AAGCTGGATT ATGTGGAGGA GCATCAGAGC CTATTCACGA ACTCAGAGAC GCTGGGCATC 
GAGGTCTTCG TCATCCCTGA GGCTGGGCTC TTTGTAGCAA CCGCCAACCG GAAAACCACA 
TCTGCCATCT ACAAGTGGAC CGATGGCAAG TTTGCTTCAT ACCAGAACAT TCCCACGCAC 
CAGGCACAGT CCTGGAGACA CTTCACCATT GGGAAAAAGA TCTTCCTGGC TGTGGCTAAT 
TTTGAGCCGA ATGAGAAGGG TCAGGAGTTC TCGGTCATTT ACAAGTGGAG CCAGAGAAGG 
CTGAGGTTCA CGCCATACCA GAGGGTCCCC ACCCACAGTG CCCGGGACTG GGAGGCCTTC 
GAGGTGGCCG GCGAGCACTT CCTGGCCGTG GCCAACCACC GGGAAGGCGA CAACCATAAC 
ATTGACAGTG TCATCTACAA GTGGAACCCA GGGACCCGGC TCTTCGAGGC CAACCAGACC 
ATCGCCACTT CGGGCGCCTA CGACTGGGAG TTCTTCACGG TGGGGCCCTA CGCCTTTCTG 
GCCGTAGCCA ACGCCTTCAA CGGCACATCC ACGCGGCTGC AGTCCCACCT GTACGTGCGG 
CTGGACGGCT CCTTCCAGCT CTTCCAGTCT TTCCTGACGT TTGGCGCCGC AGACTGGGAG 
GTTTTCCACA TTGGGGAGAG GGTCTTCCTC GCCGTGGCGA ACAGTCACCG CTACGACGTG 
GAGATGCGAG TGCAGAACGA CTCCTACGTC ATCAACTCGG TCATATACGA GCTGAACGTC 
ACCGCGCAGA CGTTCGTCCG CTTCCAGGAG ATCCGCACCT GCAGTGCTCT GGACTGGGAG 
TTCTTCTCGG TGGGAGAAGA TTATTTCCTG GTGGTTGCAA ACTCCTTCGA TGGAAATACC 
TTCTCGGTAA ACAGTATTAT TTACAGGTGG CAGGGGTATG AAGGCTTCGT GGCCGTGCAC 
AGCCTCCCAA CCTTCGGCTG CAGGGACTGG GAGGCCTTCC GCACAGCGGC CGGCTCCTTC 
CTGGTCTACT CCAGCGCCAA GGAACCGCTG TCCCGGGTGC TGAAGCTGAG GACGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857084.1
CDS	185..2044
Translation	MQVQGARGLQLSAATPRALSFPASRIFFRCDLFPEEFSIVVTLKVPSLPPKKNEYLLAVVAEERDSLLLGLRYSPTQLHFLFLSEDGAGAWQTRVSFRSPALVDGQWHVLVLAVSEGSFSLTTDCGPAVDIMADMPFPATLSVRGARFFIGSRRRTKGRFTGLVRQLVLLPGSDATPRLCPCVNPELAVLSIPAILHGLTGKPEDNEVLKYPYETNMKVTLGPRPPCTKAEDAQFWFDASRKGLYLCVGSEWVSVLAAKEKLDYVEEHQSLFTNSETLGIEVFVIPEAGLFVATANRKTTSAIYKWTDGKFASYQNIPTHQAQSWRHFTIGKKIFLAVANFEPNEKGQEFSVIYKWSQRRLRFTPYQRVPTHSARDWEAFEVAGEHFLAVANHREGDNHNIDSVIYKWNPGTRLFEANQTIATSGAYDWEFFTVGPYAFLAVANAFNGTSTRLQSHLYVRLDGSFQLFQSFLTFGAADWEVFHIGERVFLAVANSHRYDVEMRVQNDSYVINSVIYELNVTAQTFVRFQEIRTCSALDWEFFSVGEDYFLVVANSFDGNTFSVNSIIYRWQGYEGFVAVHSLPTFGCRDWEAFRTAAGSFLVYSSAKEPLSRVLKLRTG

Target ORF information:

RefSeq Version	XM_025001316.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thrombospondin type laminin G domain and EAR repeats (TSPEAR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001316.1

ORF Insert Sequence:

ATGCAAGTCC AAGGTGCACG AGGACTCCAG CTCTCAGCCG CCACGCCCCG TGCCCTGAGC 
TTCCCTGCCT CGAGGATTTT CTTCAGATGT GATCTCTTCC CGGAGGAATT TTCCATCGTT 
GTGACCTTGA AAGTCCCCAG CCTCCCCCCT AAGAAGAACG AGTACCTGCT GGCGGTGGTG 
GCAGAGGAGC GGGACTCGCT GCTGCTGGGC CTGCGATACT CGCCCACCCA GCTGCACTTC 
CTCTTCCTCA GCGAGGACGG GGCAGGCGCC TGGCAGACCC GCGTGTCCTT CCGCAGCCCG 
GCTCTGGTGG ACGGCCAGTG GCACGTGCTG GTCTTGGCCG TGTCCGAGGG CTCCTTTTCG 
CTCACTACGG ACTGCGGCCC CGCCGTGGAC ATAATGGCTG ACATGCCCTT TCCAGCCACC 
CTATCTGTGC GAGGAGCCCG GTTCTTCATC GGCAGTCGGA GGAGGACGAA AGGCCGATTC 
ACGGGCCTGG TGAGGCAGCT GGTTCTGTTG CCTGGCTCAG ATGCCACCCC GAGGCTCTGT 
CCCTGCGTGA ATCCTGAGCT CGCTGTGCTG TCCATCCCTG CTATCCTGCA TGGCCTCACA 
GGGAAGCCGG AAGACAACGA GGTGCTAAAA TACCCCTATG AAACCAACAT GAAGGTGACG 
CTGGGACCCC GGCCTCCGTG CACCAAAGCG GAGGATGCCC AGTTCTGGTT CGACGCCAGC 
CGCAAGGGGC TATACCTGTG CGTGGGCAGC GAGTGGGTCT CCGTGTTAGC AGCCAAAGAA 
AAGCTGGATT ATGTGGAGGA GCATCAGAGC CTATTCACGA ACTCAGAGAC GCTGGGCATC 
GAGGTCTTCG TCATCCCTGA GGCTGGGCTC TTTGTAGCAA CCGCCAACCG GAAAACCACA 
TCTGCCATCT ACAAGTGGAC CGATGGCAAG TTTGCTTCAT ACCAGAACAT TCCCACGCAC 
CAGGCACAGT CCTGGAGACA CTTCACCATT GGGAAAAAGA TCTTCCTGGC TGTGGCTAAT 
TTTGAGCCGA ATGAGAAGGG TCAGGAGTTC TCGGTCATTT ACAAGTGGAG CCAGAGAAGG 
CTGAGGTTCA CGCCATACCA GAGGGTCCCC ACCCACAGTG CCCGGGACTG GGAGGCCTTC 
GAGGTGGCCG GCGAGCACTT CCTGGCCGTG GCCAACCACC GGGAAGGCGA CAACCATAAC 
ATTGACAGTG TCATCTACAA GTGGAACCCA GGGACCCGGC TCTTCGAGGC CAACCAGACC 
ATCGCCACTT CGGGCGCCTA CGACTGGGAG TTCTTCACGG TGGGGCCCTA CGCCTTTCTG 
GCCGTAGCCA ACGCCTTCAA CGGCACATCC ACGCGGCTGC AGTCCCACCT GTACGTGCGG 
CTGGACGGCT CCTTCCAGCT CTTCCAGTCT TTCCTGACGT TTGGCGCCGC AGACTGGGAG 
GTTTTCCACA TTGGGGAGAG GGTCTTCCTC GCCGTGGCGA ACAGTCACCG CTACGACGTG 
GAGATGCGAG TGCAGAACGA CTCCTACGTC ATCAACTCGG TCATATACGA GCTGAACGTC 
ACCGCGCAGA CGTTCGTCCG CTTCCAGGAG ATCCGCACCT GCAGTGCTCT GGACTGGGAG 
TTCTTCTCGG TGGGAGAAGA TTATTTCCTG GTGGTTGCAA ACTCCTTCGA TGGAAATACC 
TTCTCGGTAA ACAGTATTAT TTACAGGTGG CAGGGGTATG AAGGCTTCGT GGCCGTGCAC 
AGCCTCCCAA CCTTCGGCTG CAGGGACTGG GAGGCCTTCC GCACAGCGGC CGGCTCCTTC 
CTGGTCTACT CCAGCGCCAA GGAACCGCTG TCCCGGGTGC TGAAGCTGAG GACGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defect in the gene encoding the EAR/EPTP domain-containing protein TSPEAR causes DFNB98 profound deafness.
Human molecular genetics21(17)3835-44(2012 Sep)
Delmaghani S,Aghaie A,Michalski N,Bonnet C,Weil D,Petit C

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TSPEAR cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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