HRAS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HRAS
Entrez Gene ID	100298939
Full Name	HRas proto-oncogene, GTPase
Synonyms	H-Ras
General protein information	Preferred Names HRas proto-oncogene, GTPase Names GTPase HRas GTPase HRas (Transforming protein p21) (H-Ras-1) (c-H-ras) Harvey ras 1 p19 H-RasIDX protein v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001242346.1	NP_001229275.1	GTPase HRas isoform 1
XM_024986964.1	XP_024842732.1	GTPase HRas isoform X1
XM_024986966.1	XP_024842734.1	GTPase HRas isoform X1
NM_001242347.1	NP_001229276.1	GTPase HRas isoform 2

Pathways from BioSystems

Homologies

	HRAS	XP_001085804.1
Danio rerio (zebrafish)	hrasb	NP_001018465.1
Homo sapiens (human)	HRAS	NP_005334.1
Danio rerio (zebrafish)	hrasa	NP_001017623.1
Danio rerio (zebrafish)	LOC100151000	XP_001923439.1
Mus musculus (house mouse)	Hras	NP_001123915.1
Rattus norvegicus (Norway rat)	Hras	NP_001091711.1
Gallus gallus (chicken)	HRAS	NP_990623.1
Pan troglodytes (chimpanzee)	HRAS	XP_521702.2
Canis lupus familiaris (dog)	HRAS	XP_540523.3
Bos taurus (cattle)	HRAS	NP_001229275.1
Xenopus tropicalis (tropical clawed frog)	hras	NP_001017003.1

Gene Ontology
Bibliography

Related articles in PubMed

Thrombospondin 1, fibronectin, and vitronectin are differentially dependent upon RAS, ERK1/2, and p38 for induction of vascular smooth muscle cell chemotaxis.
Willis AI, Sadowitz B, Fuse S, Maier KG, Lee TS, Wang XJ, Tuszynski GP, Sumpio BE, Gahtan V
Vascular and endovascular surgery45(1)55-62(2011 Jan)

Increased oxidative stress in diabetes regulates activation of a small molecular weight G-protein, H-Ras, in the retina.
Kowluru V, Kowluru RA
Molecular vision13602-10(2007 Apr)

Small molecular weight G-protein, H-Ras, and retinal endothelial cell apoptosis in diabetes.
Kowluru RA, Kowluru A, Kanwar M
Molecular and cellular biochemistry296(1-2)69-76(2007 Feb)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

H-ras immunohistochemical expression and molecular analysis of urinary bladder lesions in grazing adult cattle exposed to bracken fern.
Sardon D, de la Fuente I, Calonge E, Perez-Alenza MD, Casta?o M, Dunner S, Pe?a L
Journal of comparative pathology132(2-3)195-201(2005 Feb-Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thrombospondin 1, fibronectin, and vitronectin are differentially dependent upon RAS, ERK1/2, and p38 for induction of vascular smooth muscle cell chemotaxis.
Title: Thrombospondin 1, fibronectin, and vitronectin are differentially dependent upon RAS, ERK1/2, and p38 for induction of vascular smooth muscle cell chemotaxis.

Activation of H-Ras and its downstream signaling pathway in the retina and its vasculature could be under the control of superoxide, and H-Ras activation in diabetes can be prevented by inhibiting superoxide accumulation.
Title: Increased oxidative stress in diabetes regulates activation of a small molecular weight G-protein, H-Ras, in the retina.

Data demonstrate that H-Ras activation is important in the activation of the specific signaling events leading to the accelerated retinal capillary cell apoptosis in hyperglycemic conditions.
Title: Small molecular weight G-protein, H-Ras, and retinal endothelial cell apoptosis in diabetes.

The following HRAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HRAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa34960	NM_001242346.1 Latest version!	Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa38876	NM_001242347.1 Latest version!	Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa38876	XM_024986964.1 Latest version!	Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa38876	XM_024986966.1 Latest version!	Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HRAS ( NM_001242346.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001229275.1 Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa34960
Clone ID Related Accession (Same CDS sequence)	NM_001242346.1
Accession Version	NM_001242346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	GTPase HRas isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB419631.1, DN532777.1 and CO892114.1. On May 29, 2011 this sequence version replaced XM_002699432.1. Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGAGT ATAAGCTCGT GGTGGTGGGC GCCGGTGGCG TGGGGAAGAG CGCCCTGACT 
ATCCAGCTCA TTCAGAATCA CTTCGTGGAC GAGTACGACC CCACCATCGA GGACTCCTAC 
CGGAAGCAAG TGGTCATCGA TGGGGAGACG TGCCTGCTGG ACATCCTGGA CACAGCGGGC 
CAGGAGGAAT ACAGCGCCAT GCGAGACCAG TACATGCGCA CCGGGGAGGG CTTTCTCTGC 
GTGTTTGCTA TCAACCACGT CAAGTCCTTC GAGGACATCC ACCAGTACCG GGAGCAGATC 
AAGCGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGTTGG TTGGGAACAA GTGCGACCTG 
GCCGCGCGCA CCGTGGAGTC TCGGCAGGCC CAGGACCTCG CCCGCAGCTA CGGCATCCCG 
TACATCGAGA CCTCCGCCAA GACCCGCCAG GGCGTGGAGG ATGCTTTCTA CACCCTGGTG 
CGCGAGATCC GGCAGCACAA GGTGCGCAAG CTGAGCCCGC CGGACGAGGG CGGCCCCGGC 
TGCCTGAGCT GCAGGTGCCT GCTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229275.1
CDS	160..729
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINHVKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKVRKLSPPDEGGPGCLSCRCLLS

Target ORF information:

RefSeq Version	NM_001242346.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242346.1

ORF Insert Sequence:

ATGACGGAGT ATAAGCTCGT GGTGGTGGGC GCCGGTGGCG TGGGGAAGAG CGCCCTGACT 
ATCCAGCTCA TTCAGAATCA CTTCGTGGAC GAGTACGACC CCACCATCGA GGACTCCTAC 
CGGAAGCAAG TGGTCATCGA TGGGGAGACG TGCCTGCTGG ACATCCTGGA CACAGCGGGC 
CAGGAGGAAT ACAGCGCCAT GCGAGACCAG TACATGCGCA CCGGGGAGGG CTTTCTCTGC 
GTGTTTGCTA TCAACCACGT CAAGTCCTTC GAGGACATCC ACCAGTACCG GGAGCAGATC 
AAGCGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGTTGG TTGGGAACAA GTGCGACCTG 
GCCGCGCGCA CCGTGGAGTC TCGGCAGGCC CAGGACCTCG CCCGCAGCTA CGGCATCCCG 
TACATCGAGA CCTCCGCCAA GACCCGCCAG GGCGTGGAGG ATGCTTTCTA CACCCTGGTG 
CGCGAGATCC GGCAGCACAA GGTGCGCAAG CTGAGCCCGC CGGACGAGGG CGGCCCCGGC 
TGCCTGAGCT GCAGGTGCCT GCTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HRAS ( NM_001242347.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001229276.1 Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa38876
Clone ID Related Accession (Same CDS sequence)	XM_024986964.1 , XM_024986966.1 , NM_001242347.1
Accession Version	NM_001242347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	GTPase HRas isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB419631.1, DY123658.1 and CO892114.1. Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGAGT ATAAGCTCGT GGTGGTGGGC GCCGGTGGCG TGGGGAAGAG CGCCCTGACT 
ATCCAGCTCA TTCAGAATCA CTTCGTGGAC GAGTACGACC CCACCATCGA GGACTCCTAC 
CGGAAGCAAG TGGTCATCGA TGGGGAGACG TGCCTGCTGG ACATCCTGGA CACAGCGGGC 
CAGGAGGAAT ACAGCGCCAT GCGAGACCAG TACATGCGCA CCGGGGAGGG CTTTCTCTGC 
GTGTTTGCTA TCAACCACGT CAAGTCCTTC GAGGACATCC ACCAGTACCG GGAGCAGATC 
AAGCGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGTTGG TTGGGAACAA GTGCGACCTG 
GCCGCGCGCA CCGTGGAGTC TCGGCAGGCC CAGGACCTCG CCCGCAGCTA CGGCATCCCG 
TACATCGAGA CCTCCGCCAA GACCCGCCAG GGCAGCCGCT CTGGCTCTGG CTCCAGCTCC 
GGGACCCTCT GGGACCCTCC GGGACCCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229276.1
CDS	160..672
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINHVKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGSRSGSGSSSGTLWDPPGPP

Target ORF information:

RefSeq Version	NM_001242347.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242347.1

ORF Insert Sequence:

ATGACGGAGT ATAAGCTCGT GGTGGTGGGC GCCGGTGGCG TGGGGAAGAG CGCCCTGACT 
ATCCAGCTCA TTCAGAATCA CTTCGTGGAC GAGTACGACC CCACCATCGA GGACTCCTAC 
CGGAAGCAAG TGGTCATCGA TGGGGAGACG TGCCTGCTGG ACATCCTGGA CACAGCGGGC 
CAGGAGGAAT ACAGCGCCAT GCGAGACCAG TACATGCGCA CCGGGGAGGG CTTTCTCTGC 
GTGTTTGCTA TCAACCACGT CAAGTCCTTC GAGGACATCC ACCAGTACCG GGAGCAGATC 
AAGCGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGTTGG TTGGGAACAA GTGCGACCTG 
GCCGCGCGCA CCGTGGAGTC TCGGCAGGCC CAGGACCTCG CCCGCAGCTA CGGCATCCCG 
TACATCGAGA CCTCCGCCAA GACCCGCCAG GGCAGCCGCT CTGGCTCTGG CTCCAGCTCC 
GGGACCCTCT GGGACCCTCC GGGACCCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HRAS ( XM_024986964.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842732.1 Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa38876
Clone ID Related Accession (Same CDS sequence)	XM_024986964.1 , XM_024986966.1 , NM_001242347.1
Accession Version	XM_024986964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GTPase HRas isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGT ATAAGCTCGT GGTGGTGGGC GCCGGTGGCG TGGGGAAGAG CGCCCTGACT 
ATCCAGCTCA TTCAGAATCA CTTCGTGGAC GAGTACGACC CCACCATCGA GGACTCCTAC 
CGGAAGCAAG TGGTCATCGA TGGGGAGACG TGCCTGCTGG ACATCCTGGA CACAGCGGGC 
CAGGAGGAAT ACAGCGCCAT GCGAGACCAG TACATGCGCA CCGGGGAGGG CTTTCTCTGC 
GTGTTTGCTA TCAACCACGT CAAGTCCTTC GAGGACATCC ACCAGTACCG GGAGCAGATC 
AAGCGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGTTGG TTGGGAACAA GTGCGACCTG 
GCCGCGCGCA CCGTGGAGTC TCGGCAGGCC CAGGACCTCG CCCGCAGCTA CGGCATCCCG 
TACATCGAGA CCTCCGCCAA GACCCGCCAG GGCAGCCGCT CTGGCTCTGG CTCCAGCTCC 
GGGACCCTCT GGGACCCTCC GGGACCCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842732.1
CDS	195..707
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINHVKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGSRSGSGSSSGTLWDPPGPP

Target ORF information:

RefSeq Version	XM_024986964.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986964.1

ORF Insert Sequence:

ATGACGGAGT ATAAGCTCGT GGTGGTGGGC GCCGGTGGCG TGGGGAAGAG CGCCCTGACT 
ATCCAGCTCA TTCAGAATCA CTTCGTGGAC GAGTACGACC CCACCATCGA GGACTCCTAC 
CGGAAGCAAG TGGTCATCGA TGGGGAGACG TGCCTGCTGG ACATCCTGGA CACAGCGGGC 
CAGGAGGAAT ACAGCGCCAT GCGAGACCAG TACATGCGCA CCGGGGAGGG CTTTCTCTGC 
GTGTTTGCTA TCAACCACGT CAAGTCCTTC GAGGACATCC ACCAGTACCG GGAGCAGATC 
AAGCGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGTTGG TTGGGAACAA GTGCGACCTG 
GCCGCGCGCA CCGTGGAGTC TCGGCAGGCC CAGGACCTCG CCCGCAGCTA CGGCATCCCG 
TACATCGAGA CCTCCGCCAA GACCCGCCAG GGCAGCCGCT CTGGCTCTGG CTCCAGCTCC 
GGGACCCTCT GGGACCCTCC GGGACCCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HRAS ( XM_024986966.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842734.1 Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa38876
Clone ID Related Accession (Same CDS sequence)	XM_024986964.1 , XM_024986966.1 , NM_001242347.1
Accession Version	XM_024986966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GTPase HRas isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGT ATAAGCTCGT GGTGGTGGGC GCCGGTGGCG TGGGGAAGAG CGCCCTGACT 
ATCCAGCTCA TTCAGAATCA CTTCGTGGAC GAGTACGACC CCACCATCGA GGACTCCTAC 
CGGAAGCAAG TGGTCATCGA TGGGGAGACG TGCCTGCTGG ACATCCTGGA CACAGCGGGC 
CAGGAGGAAT ACAGCGCCAT GCGAGACCAG TACATGCGCA CCGGGGAGGG CTTTCTCTGC 
GTGTTTGCTA TCAACCACGT CAAGTCCTTC GAGGACATCC ACCAGTACCG GGAGCAGATC 
AAGCGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGTTGG TTGGGAACAA GTGCGACCTG 
GCCGCGCGCA CCGTGGAGTC TCGGCAGGCC CAGGACCTCG CCCGCAGCTA CGGCATCCCG 
TACATCGAGA CCTCCGCCAA GACCCGCCAG GGCAGCCGCT CTGGCTCTGG CTCCAGCTCC 
GGGACCCTCT GGGACCCTCC GGGACCCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842734.1
CDS	964..1476
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINHVKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGSRSGSGSSSGTLWDPPGPP

Target ORF information:

RefSeq Version	XM_024986966.1
Organism	Bos taurus(cattle)
Definition	Bos taurus HRas proto-oncogene, GTPase (HRAS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986966.1

ORF Insert Sequence:

ATGACGGAGT ATAAGCTCGT GGTGGTGGGC GCCGGTGGCG TGGGGAAGAG CGCCCTGACT 
ATCCAGCTCA TTCAGAATCA CTTCGTGGAC GAGTACGACC CCACCATCGA GGACTCCTAC 
CGGAAGCAAG TGGTCATCGA TGGGGAGACG TGCCTGCTGG ACATCCTGGA CACAGCGGGC 
CAGGAGGAAT ACAGCGCCAT GCGAGACCAG TACATGCGCA CCGGGGAGGG CTTTCTCTGC 
GTGTTTGCTA TCAACCACGT CAAGTCCTTC GAGGACATCC ACCAGTACCG GGAGCAGATC 
AAGCGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGTTGG TTGGGAACAA GTGCGACCTG 
GCCGCGCGCA CCGTGGAGTC TCGGCAGGCC CAGGACCTCG CCCGCAGCTA CGGCATCCCG 
TACATCGAGA CCTCCGCCAA GACCCGCCAG GGCAGCCGCT CTGGCTCTGG CTCCAGCTCC 
GGGACCCTCT GGGACCCTCC GGGACCCCCG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Thrombospondin 1, fibronectin, and vitronectin are differentially dependent upon RAS, ERK1/2, and p38 for induction of vascular smooth muscle cell chemotaxis.
Vascular and endovascular surgery45(1)55-62(2011 Jan)
Willis AI,Sadowitz B,Fuse S,Maier KG,Lee TS,Wang XJ,Tuszynski GP,Sumpio BE,Gahtan V

Increased oxidative stress in diabetes regulates activation of a small molecular weight G-protein, H-Ras, in the retina.
Molecular vision13602-10(2007 Apr)
Kowluru V,Kowluru RA

Small molecular weight G-protein, H-Ras, and retinal endothelial cell apoptosis in diabetes.
Molecular and cellular biochemistry296(1-2)69-76(2007 Feb)
Kowluru RA,Kowluru A,Kanwar M

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

H-ras immunohistochemical expression and molecular analysis of urinary bladder lesions in grazing adult cattle exposed to bracken fern.
Journal of comparative pathology132(2-3)195-201(2005 Feb-Apr)
Sardon D,de la Fuente I,Calonge E,Perez-Alenza MD,Casta?o M,Dunner S,Pe?a L

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HRAS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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