MAGI2 cDNA ORF clone, Bos taurus(cattle)

The following MAGI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa233879 XM_024990949.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa89704 XM_015468987.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89704 XM_015463003.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89705 XM_015463004.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89705 XM_015468988.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89706 XM_015468989.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89706 XM_015463005.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89707 XM_015463006.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89707 XM_015468990.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa89708 XM_015463007.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89708 XM_015468991.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89709 XM_015468992.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89709 XM_015463008.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89710 XM_015463009.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89710 XM_015468993.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89711 XM_015468994.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89711 XM_015463010.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89712 XM_015463011.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89712 XM_015468995.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa89713 XM_015468996.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa89713 XM_015463012.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa89714 XM_015468997.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa89714 XM_015463013.1
Latest version!
Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa233879
    Clone ID Related Accession (Same CDS sequence) XM_024990949.1
    Accession Version XM_024990949.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAAATC AAATTACATA CTTATTTTCC AATTGTATCA TTCTAGGAGG AATTGTTGAT 
    AAGGATCTTC GTCACTACCT CAACTTACGA TTTCAGAAGG GGTCTGTGGA CCATGAACTT
    CAGCAAATCA TTCGTGACAA CCTCTACCTC CGCACGGTGC CATGCACCAC AAGGCCACAT
    AAGGAGGGTG AGGTCCCTGG AGTGGACTAT ATTTTCATCA CTGTTGAAGA TTTTATGGAA
    TTGGAGAAAA GCGGTGCTCT CCTAGAAAGT GGGACTTATG AAGACAATTA CTACGGTACC
    CCAAAGCCTC CAGCAGAACC AGCACCATTA TTATTAAATG TAACAGACCA GATACTTCCT
    GGAGCCACTC CAAGCGCTGA AGGAAAACGA AAGAGGAATA AATCAGTGAG CAACATGGAG
    AAAGCAAGCA TAGAGCCTCC TGAGGAGGAA GAGGAAGAGA GGCCTGTGGT CAATGGAAAT
    GGAGTAGTAG TGACACCAGA ATCCAGTGAA CCTGAAGACA AGAGTGCAGG TGCCTCAGGG
    GAGACACCCT CCCAGCCTTA TCCTGCACCA GTGTACAGTC AGCCCGAGGA GGTGAAGGAG
    CAGATGGATG ACGCGAAACC AACTAAACCT GAGGAGAATG AGGACTCAGA CCCATTGCCT
    GACAACTGGG AAATGGCTTA TACAGAGAAG GGTGAAGTCT ACTTCATTGA CCATAACACA
    AAGACAACAT CATGGCTGGA TCCACGACTT GCGAAAAAGG CTAAACCTCC AGAAGAGTGC
    AAAGAAAATG AGCTTCCATA TGGCTGGGAA AAAATCGATG ATCCCATATA TGGCACTTAT
    TATGTCGACC ACATAAATAG AAGAACACAG TTTGAAAACC CTGTCCTGGA AGCAAAAAGG
    AAGCTACAGC AGCATAACAT GCCCCACACA GAACTTGGAA CAAAGCCCTT GCAGGCCCCA
    GGTTTCCGAG TAGACAGCTC CTCATCGACA GTACCTAGGC CCAAACCTGC CCGCTCCCTC
    GCCGCCCGGG AGCGGTCCCG CAGCGTGAGT GACCTGTCTT ACTACGGGAG CCACCTCCCC
    GGGAGGCTCT GGCGCATCGA AAAGCCACTC TTTACCCGGG ATGCCTCCCA ATTGAAGGGA
    ACGTTTCTCA GCACCACACT CAAAAAGAGC AACATGGGGT TTGGATTTAC CATTATTGGC
    GGAGATGAGC CTGACGAGTT TCTGCAGGTG AAAAGTGTGA TTCCCGACGG GCCTGCAGCA
    CAAGACGGGA AAATGGAAAC AGGTGATGTC ATTGTCTATA TTAATGAAGT TTGTGTCCTT
    GGACACACTC ATGCAGATGT AGTCAAACTT TTCCAGTCTG TTCCTATTGG TCAGAGTGTC
    AACTTGGTGT TGTGTCGTGG CTACCCTTTG CCCTTTGATC CTGAAGATCC TGCTAACAGC
    ATGGTGCCAC CCCTTGCAAT AATGGAGAGG CCACCTCCAG TGATGGTCAA TGGAAGACAT
    AACTATGAAA CATATTTGGA ATACATTTCT CGGACCTCAC AGTCAGTTCC GGATATAACA
    GATCGGCCGC CTCATACTTT GCACTCAATG CCAGCTGATG GTCAGCTAGA TGGCACGTAT
    CCACCACCTG TCCATGACGA CAATGTATCT ATGGCTTCAT CTGGGGCCAC CCAAGCTGAA
    CTTATGACCT TAACCATTGT GAAAGGTGCC CAGGGTTTTG GCTTCACCAT TGCAGACAGT
    CCTACAGGAC AGCGGGTGAA ACAAATACTT GACATTCAGG GATGCCCTGG CCTGTGTGAA
    GGCGACCTCA TTATTGAGAT CAACCAGCAG AATGTACAGA ACCTGAGCCA TACGGAAGTA
    GTGGATATAC TTAAGGACTG TCCCATTGGA AGCGAGACTT CTTTGATTAT TCATCGAGGA
    GGTTTCTTTT CTCCATGGAA AACCCCAAAG CCTATAATGG ATCGATGGGA GAACCAAGGC
    AGTCCTCAAA CGAGTTTATC TGCTCCGGCC ATACCACAGA ACCTGCCCTT CCCACCCGCC
    CTTCACAGGA GTTCCTTTCC TGACTCAACA GAGGCCTTTG ACCCAAGGAA GCCTGACCCA
    TATGAGCTCT ACGAGAAATC GAGAGCCATT TATGAAAGTA GGCAACAAGT GCCACCAAGG
    ACCAGTTTTC GAATGGATTC TTCTGGTCCA GATTATAAGG AGCTGGATGT TCACCTTCGC
    AGGATGGAGT CTGGATTTGG CTTCAGAATC CTCGGGGGAG ATGAGCCTGG ACAGCCTATT
    TTGATTGGAG CCGTCATCGC CATGGGCTCA GCTGACAGAG ATGGCCGCCT ACACCCAGGA
    GATGAGCTCG TCTATGTGGA TGGAATTCCA GTGGCTGGAA AGACCCACCG CTATGTCATT
    GACCTTATGC ACCATGCAGC CCGGAATGGG CAGGTCAACC TCACTGTGAG AAGAAAGGTG
    CTATGTGGAG GGGAGCCCTG CCCAGAGAAT GGGAGGAGTC CAGGCTCCGT GTCGACCCAC
    CACAGTTCTC CTCGCAGTGA CTACGCAACC TACACCAACA CCTACAGCAA CAGTAACCAT
    GCCGTCCCCA CCAGCAACGC CTCTCCCCCC GAAGGCTTCG CCTCCCACAG CCTGCAGACC
    AGCGACGTGG TCATTCACCG CAAAGAGAAC GAGGGTTTCG GCTTTGTCAT CATCAGCTCC
    CTGAACAGGC CAGAGTCTGG ATCCACTATA ACTGTGCCAC ATAAAATTGG ACGCATCATT
    GATGGGAGTC CCGCAGATCG CTGTGCAAAG CTAAAAGTGG GAGACCGGAT CTTAGCGGTG
    AACGGCCAGT CTATCATCAA CATGCCTCAT GCAGACATCG TGAAGCTCAT CAAGGATGCG
    GGTCTTAGCG TCACCCTTCG CATCATCCCT CAGGAGGAAC TCAACAGCCC CGCTTCAGCA
    CCCAGCTCAG AGAAGCAGAG CCCCCTGGCC CAGCAGCACA GCCCCCTGGC CCAACAGAGT
    CCTCTGGCCC AGCCCAGCCC GGCCACGCCC AACAGTCCCA TCGCCCAGCC AGCTCCGCCT
    CAGCCTCTTC AGCCTCAAGG ACATGAAAAT AGTTACAGGT CAGAAGTAAA AGCGAGGCAA
    GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACGGACT ACAGGCAGCC TCCACTGGAC
    TACAGGCAAC CCCCAGGAGG GGACTACCCG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
    CCGCTACTGG ACTACAGGCA GCACTCCCCA GACACCAGGC AGTTCCCTCT GTCAGACTAC
    AGGCAGCCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
    GGATTCAGCA TTCGTGGAGG AAGGGAGTAC AAAATGGATT TGTATGTGCT GAGATTAGCA
    GAAGATGGGC CAGCAATAAG GAATGGAAGG ATGAGGGTAG GAGATCAGAT CATTGAAATC
    AATGGGGAGA GCACAAGAGA CATGACCCAT GCCAGAGCAA TAGAGCTCAT CAAATCAGGC
    GGAAGGAGAG TGAGGCTACT GCTGAAGCGT GGGACGGGAC AGGTCCCAGA GTATGACGAA
    CCCGGCTCCT GGAGTGCTCC CGCTGCCGCC TCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
    CTGGAGGACG TCCTCTCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
    CACCAGACAA ACCCAGACCC AACTTGGGAT ATCAAAAGGG AACACGACGT TAGGAAACCA
    AAGGAGCTTT CAGCCGGCAG CCAGAAGAAG CAGCGCCTTG GGGAGCAGAG CGAGCGCTCA
    GCCAGCCCGC AAAGGGCCGT GCAACCAGGG CTCGAGGAGG CGCCTGGCGG CCAGGGGCAG
    CCTGAGGCCG GCAGGCCCTC CTCGGAGACC AGAGCGCCTG GGCCCGCACT GGCCGAAGCG
    GCGCGGGCTG GCAACAAGGA GGGGCTGCAG GCCCCGGTGG GCTCCGAGCT CGGCCGGCAC
    GGAGGCCCCG GCGCGGCACC TGCGTTCGTG GGCCCGGGCC GCGGCGGGGG CGCACGGGAG
    GTCGAGGGCC GGGCCGGGGC GCGGGCGGGG TCCAGGCAGG GCTCGCGGCC CCCCGGGGGC
    GCCCCTGCGC GCAAGGCCGC CGTGGCGCCC GGGCCGTGGA AGGTGCCGGG CTCCGACAAG
    CTGCCGGGCG TCCTCAAACC CGGCGCCTCA GCCCCGGGGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846717.1
    CDS1..4245
    Translation

    Target ORF information:

    RefSeq Version XM_024990949.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990949.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGTCAAATC AAATTACATA CTTATTTTCC AATTGTATCA TTCTAGGAGG AATTGTTGAT 
    AAGGATCTTC GTCACTACCT CAACTTACGA TTTCAGAAGG GGTCTGTGGA CCATGAACTT
    CAGCAAATCA TTCGTGACAA CCTCTACCTC CGCACGGTGC CATGCACCAC AAGGCCACAT
    AAGGAGGGTG AGGTCCCTGG AGTGGACTAT ATTTTCATCA CTGTTGAAGA TTTTATGGAA
    TTGGAGAAAA GCGGTGCTCT CCTAGAAAGT GGGACTTATG AAGACAATTA CTACGGTACC
    CCAAAGCCTC CAGCAGAACC AGCACCATTA TTATTAAATG TAACAGACCA GATACTTCCT
    GGAGCCACTC CAAGCGCTGA AGGAAAACGA AAGAGGAATA AATCAGTGAG CAACATGGAG
    AAAGCAAGCA TAGAGCCTCC TGAGGAGGAA GAGGAAGAGA GGCCTGTGGT CAATGGAAAT
    GGAGTAGTAG TGACACCAGA ATCCAGTGAA CCTGAAGACA AGAGTGCAGG TGCCTCAGGG
    GAGACACCCT CCCAGCCTTA TCCTGCACCA GTGTACAGTC AGCCCGAGGA GGTGAAGGAG
    CAGATGGATG ACGCGAAACC AACTAAACCT GAGGAGAATG AGGACTCAGA CCCATTGCCT
    GACAACTGGG AAATGGCTTA TACAGAGAAG GGTGAAGTCT ACTTCATTGA CCATAACACA
    AAGACAACAT CATGGCTGGA TCCACGACTT GCGAAAAAGG CTAAACCTCC AGAAGAGTGC
    AAAGAAAATG AGCTTCCATA TGGCTGGGAA AAAATCGATG ATCCCATATA TGGCACTTAT
    TATGTCGACC ACATAAATAG AAGAACACAG TTTGAAAACC CTGTCCTGGA AGCAAAAAGG
    AAGCTACAGC AGCATAACAT GCCCCACACA GAACTTGGAA CAAAGCCCTT GCAGGCCCCA
    GGTTTCCGAG TAGACAGCTC CTCATCGACA GTACCTAGGC CCAAACCTGC CCGCTCCCTC
    GCCGCCCGGG AGCGGTCCCG CAGCGTGAGT GACCTGTCTT ACTACGGGAG CCACCTCCCC
    GGGAGGCTCT GGCGCATCGA AAAGCCACTC TTTACCCGGG ATGCCTCCCA ATTGAAGGGA
    ACGTTTCTCA GCACCACACT CAAAAAGAGC AACATGGGGT TTGGATTTAC CATTATTGGC
    GGAGATGAGC CTGACGAGTT TCTGCAGGTG AAAAGTGTGA TTCCCGACGG GCCTGCAGCA
    CAAGACGGGA AAATGGAAAC AGGTGATGTC ATTGTCTATA TTAATGAAGT TTGTGTCCTT
    GGACACACTC ATGCAGATGT AGTCAAACTT TTCCAGTCTG TTCCTATTGG TCAGAGTGTC
    AACTTGGTGT TGTGTCGTGG CTACCCTTTG CCCTTTGATC CTGAAGATCC TGCTAACAGC
    ATGGTGCCAC CCCTTGCAAT AATGGAGAGG CCACCTCCAG TGATGGTCAA TGGAAGACAT
    AACTATGAAA CATATTTGGA ATACATTTCT CGGACCTCAC AGTCAGTTCC GGATATAACA
    GATCGGCCGC CTCATACTTT GCACTCAATG CCAGCTGATG GTCAGCTAGA TGGCACGTAT
    CCACCACCTG TCCATGACGA CAATGTATCT ATGGCTTCAT CTGGGGCCAC CCAAGCTGAA
    CTTATGACCT TAACCATTGT GAAAGGTGCC CAGGGTTTTG GCTTCACCAT TGCAGACAGT
    CCTACAGGAC AGCGGGTGAA ACAAATACTT GACATTCAGG GATGCCCTGG CCTGTGTGAA
    GGCGACCTCA TTATTGAGAT CAACCAGCAG AATGTACAGA ACCTGAGCCA TACGGAAGTA
    GTGGATATAC TTAAGGACTG TCCCATTGGA AGCGAGACTT CTTTGATTAT TCATCGAGGA
    GGTTTCTTTT CTCCATGGAA AACCCCAAAG CCTATAATGG ATCGATGGGA GAACCAAGGC
    AGTCCTCAAA CGAGTTTATC TGCTCCGGCC ATACCACAGA ACCTGCCCTT CCCACCCGCC
    CTTCACAGGA GTTCCTTTCC TGACTCAACA GAGGCCTTTG ACCCAAGGAA GCCTGACCCA
    TATGAGCTCT ACGAGAAATC GAGAGCCATT TATGAAAGTA GGCAACAAGT GCCACCAAGG
    ACCAGTTTTC GAATGGATTC TTCTGGTCCA GATTATAAGG AGCTGGATGT TCACCTTCGC
    AGGATGGAGT CTGGATTTGG CTTCAGAATC CTCGGGGGAG ATGAGCCTGG ACAGCCTATT
    TTGATTGGAG CCGTCATCGC CATGGGCTCA GCTGACAGAG ATGGCCGCCT ACACCCAGGA
    GATGAGCTCG TCTATGTGGA TGGAATTCCA GTGGCTGGAA AGACCCACCG CTATGTCATT
    GACCTTATGC ACCATGCAGC CCGGAATGGG CAGGTCAACC TCACTGTGAG AAGAAAGGTG
    CTATGTGGAG GGGAGCCCTG CCCAGAGAAT GGGAGGAGTC CAGGCTCCGT GTCGACCCAC
    CACAGTTCTC CTCGCAGTGA CTACGCAACC TACACCAACA CCTACAGCAA CAGTAACCAT
    GCCGTCCCCA CCAGCAACGC CTCTCCCCCC GAAGGCTTCG CCTCCCACAG CCTGCAGACC
    AGCGACGTGG TCATTCACCG CAAAGAGAAC GAGGGTTTCG GCTTTGTCAT CATCAGCTCC
    CTGAACAGGC CAGAGTCTGG ATCCACTATA ACTGTGCCAC ATAAAATTGG ACGCATCATT
    GATGGGAGTC CCGCAGATCG CTGTGCAAAG CTAAAAGTGG GAGACCGGAT CTTAGCGGTG
    AACGGCCAGT CTATCATCAA CATGCCTCAT GCAGACATCG TGAAGCTCAT CAAGGATGCG
    GGTCTTAGCG TCACCCTTCG CATCATCCCT CAGGAGGAAC TCAACAGCCC CGCTTCAGCA
    CCCAGCTCAG AGAAGCAGAG CCCCCTGGCC CAGCAGCACA GCCCCCTGGC CCAACAGAGT
    CCTCTGGCCC AGCCCAGCCC GGCCACGCCC AACAGTCCCA TCGCCCAGCC AGCTCCGCCT
    CAGCCTCTTC AGCCTCAAGG ACATGAAAAT AGTTACAGGT CAGAAGTAAA AGCGAGGCAA
    GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACGGACT ACAGGCAGCC TCCACTGGAC
    TACAGGCAAC CCCCAGGAGG GGACTACCCG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
    CCGCTACTGG ACTACAGGCA GCACTCCCCA GACACCAGGC AGTTCCCTCT GTCAGACTAC
    AGGCAGCCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
    GGATTCAGCA TTCGTGGAGG AAGGGAGTAC AAAATGGATT TGTATGTGCT GAGATTAGCA
    GAAGATGGGC CAGCAATAAG GAATGGAAGG ATGAGGGTAG GAGATCAGAT CATTGAAATC
    AATGGGGAGA GCACAAGAGA CATGACCCAT GCCAGAGCAA TAGAGCTCAT CAAATCAGGC
    GGAAGGAGAG TGAGGCTACT GCTGAAGCGT GGGACGGGAC AGGTCCCAGA GTATGACGAA
    CCCGGCTCCT GGAGTGCTCC CGCTGCCGCC TCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
    CTGGAGGACG TCCTCTCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
    CACCAGACAA ACCCAGACCC AACTTGGGAT ATCAAAAGGG AACACGACGT TAGGAAACCA
    AAGGAGCTTT CAGCCGGCAG CCAGAAGAAG CAGCGCCTTG GGGAGCAGAG CGAGCGCTCA
    GCCAGCCCGC AAAGGGCCGT GCAACCAGGG CTCGAGGAGG CGCCTGGCGG CCAGGGGCAG
    CCTGAGGCCG GCAGGCCCTC CTCGGAGACC AGAGCGCCTG GGCCCGCACT GGCCGAAGCG
    GCGCGGGCTG GCAACAAGGA GGGGCTGCAG GCCCCGGTGG GCTCCGAGCT CGGCCGGCAC
    GGAGGCCCCG GCGCGGCACC TGCGTTCGTG GGCCCGGGCC GCGGCGGGGG CGCACGGGAG
    GTCGAGGGCC GGGCCGGGGC GCGGGCGGGG TCCAGGCAGG GCTCGCGGCC CCCCGGGGGC
    GCCCCTGCGC GCAAGGCCGC CGTGGCGCCC GGGCCGTGGA AGGTGCCGGG CTCCGACAAG
    CTGCCGGGCG TCCTCAAACC CGGCGCCTCA GCCCCGGGGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89704
    Clone ID Related Accession (Same CDS sequence) XM_015468987.1 , XM_015463003.1
    Accession Version XM_015468987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCAACAAGTG
    CCACCAAGGA CCAGTTTTCG AATGGATTCT TCTGGTCCAG ATTATAAGGA GCTGGATGTT
    CACCTTCGCA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
    CAGCCTATTT TGATTGGAGC CGTCATCGCC ATGGGCTCAG CTGACAGAGA TGGCCGCCTA
    CACCCAGGAG ATGAGCTCGT CTATGTGGAT GGAATTCCAG TGGCTGGAAA GACCCACCGC
    TATGTCATTG ACCTTATGCA CCATGCAGCC CGGAATGGGC AGGTCAACCT CACTGTGAGA
    AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAATG GGAGGAGTCC AGGCTCCGTG
    TCGACCCACC ACAGTTCTCC TCGCAGTGAC TACGCAACCT ACACCAACAC CTACAGCAAC
    AGTAACCATG CCGTCCCCAC CAGCAACGCC TCTCCCCCCG AAGGCTTCGC CTCCCACAGC
    CTGCAGACCA GCGACGTGGT CATTCACCGC AAAGAGAACG AGGGTTTCGG CTTTGTCATC
    ATCAGCTCCC TGAACAGGCC AGAGTCTGGA TCCACTATAA CTGTGCCACA TAAAATTGGA
    CGCATCATTG ATGGGAGTCC CGCAGATCGC TGTGCAAAGC TAAAAGTGGG AGACCGGATC
    TTAGCGGTGA ACGGCCAGTC TATCATCAAC ATGCCTCATG CAGACATCGT GAAGCTCATC
    AAGGATGCGG GTCTTAGCGT CACCCTTCGC ATCATCCCTC AGGAGGAACT CAACAGCCCC
    GCTTCAGCAC CCAGCTCAGA GAAGCAGAGC CCCCTGGCCC AGCAGCACAG CCCCCTGGCC
    CAACAGAGTC CTCTGGCCCA GCCCAGCCCG GCCACGCCCA ACAGTCCCAT CGCCCAGCCA
    GCTCCGCCTC AGCCTCTTCA GCCTCAAGGA CATGAAAATA GTTACAGGTC AGAAGTAAAA
    GCGAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACGGACTA CAGGCAGCCT
    CCACTGGACT ACAGGCAACC CCCAGGAGGG GACTACCCGC AGCCCCCACC CTTGGACTAC
    AGGCAGCCTC CGCTACTGGA CTACAGGCAG CACTCCCCAG ACACCAGGCA GTTCCCTCTG
    TCAGACTACA GGCAGCCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
    AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAGTACA AAATGGATTT GTATGTGCTG
    AGATTAGCAG AAGATGGGCC AGCAATAAGG AATGGAAGGA TGAGGGTAGG AGATCAGATC
    ATTGAAATCA ATGGGGAGAG CACAAGAGAC ATGACCCATG CCAGAGCAAT AGAGCTCATC
    AAATCAGGCG GAAGGAGAGT GAGGCTACTG CTGAAGCGTG GGACGGGACA GGTCCCAGAG
    TATGACGAAC CCGGCTCCTG GAGTGCTCCC GCTGCCGCCT CCCCAGGTCT GCCGGAAGTA
    GGCGTCTCCC TGGAGGACGT CCTCTCTCCA TTCTCTCCAT CACATCCAGC CCCACCCTCC
    GACCCTTCCC ACCAGACAAA CCCAGACCCA ACTTGGGATA TCAAAAGGGA ACACGACGTT
    AGGAAACCAA AGGAGCTTTC AGCCGGCAGC CAGAAGAAGC AGCGCCTTGG GGAGCAGAGC
    GAGCGCTCAG CCAGCCCGCA AAGGGCCGTG CAACCAGGGC TCGAGGAGGC GCCTGGCGGC
    CAGGGGCAGC CTGAGGCCGG CAGGCCCTCC TCGGAGACCA GAGCGCCTGG GCCCGCACTG
    GCCGAAGCGG CGCGGGCTGG CAACAAGGAG GGGCTGCAGG CCCCGGTGGG CTCCGAGCTC
    GGCCGGCACG GAGGCCCCGG CGCGGCACCT GCGTTCGTGG GCCCGGGCCG CGGCGGGGGC
    GCACGGGAGG TCGAGGGCCG GGCCGGGGCG CGGGCGGGGT CCAGGCAGGG CTCGCGGCCC
    CCCGGGGGCG CCCCTGCGCG CAAGGCCGCC GTGGCGCCCG GGCCGTGGAA GGTGCCGGGC
    TCCGACAAGC TGCCGGGCGT CCTCAAACCC GGCGCCTCAG CCCCGGGGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324473.1
    CDS920..4933
    Translation

    Target ORF information:

    RefSeq Version XM_015468987.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCAACAAGTG
    CCACCAAGGA CCAGTTTTCG AATGGATTCT TCTGGTCCAG ATTATAAGGA GCTGGATGTT
    CACCTTCGCA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
    CAGCCTATTT TGATTGGAGC CGTCATCGCC ATGGGCTCAG CTGACAGAGA TGGCCGCCTA
    CACCCAGGAG ATGAGCTCGT CTATGTGGAT GGAATTCCAG TGGCTGGAAA GACCCACCGC
    TATGTCATTG ACCTTATGCA CCATGCAGCC CGGAATGGGC AGGTCAACCT CACTGTGAGA
    AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAATG GGAGGAGTCC AGGCTCCGTG
    TCGACCCACC ACAGTTCTCC TCGCAGTGAC TACGCAACCT ACACCAACAC CTACAGCAAC
    AGTAACCATG CCGTCCCCAC CAGCAACGCC TCTCCCCCCG AAGGCTTCGC CTCCCACAGC
    CTGCAGACCA GCGACGTGGT CATTCACCGC AAAGAGAACG AGGGTTTCGG CTTTGTCATC
    ATCAGCTCCC TGAACAGGCC AGAGTCTGGA TCCACTATAA CTGTGCCACA TAAAATTGGA
    CGCATCATTG ATGGGAGTCC CGCAGATCGC TGTGCAAAGC TAAAAGTGGG AGACCGGATC
    TTAGCGGTGA ACGGCCAGTC TATCATCAAC ATGCCTCATG CAGACATCGT GAAGCTCATC
    AAGGATGCGG GTCTTAGCGT CACCCTTCGC ATCATCCCTC AGGAGGAACT CAACAGCCCC
    GCTTCAGCAC CCAGCTCAGA GAAGCAGAGC CCCCTGGCCC AGCAGCACAG CCCCCTGGCC
    CAACAGAGTC CTCTGGCCCA GCCCAGCCCG GCCACGCCCA ACAGTCCCAT CGCCCAGCCA
    GCTCCGCCTC AGCCTCTTCA GCCTCAAGGA CATGAAAATA GTTACAGGTC AGAAGTAAAA
    GCGAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACGGACTA CAGGCAGCCT
    CCACTGGACT ACAGGCAACC CCCAGGAGGG GACTACCCGC AGCCCCCACC CTTGGACTAC
    AGGCAGCCTC CGCTACTGGA CTACAGGCAG CACTCCCCAG ACACCAGGCA GTTCCCTCTG
    TCAGACTACA GGCAGCCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
    AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAGTACA AAATGGATTT GTATGTGCTG
    AGATTAGCAG AAGATGGGCC AGCAATAAGG AATGGAAGGA TGAGGGTAGG AGATCAGATC
    ATTGAAATCA ATGGGGAGAG CACAAGAGAC ATGACCCATG CCAGAGCAAT AGAGCTCATC
    AAATCAGGCG GAAGGAGAGT GAGGCTACTG CTGAAGCGTG GGACGGGACA GGTCCCAGAG
    TATGACGAAC CCGGCTCCTG GAGTGCTCCC GCTGCCGCCT CCCCAGGTCT GCCGGAAGTA
    GGCGTCTCCC TGGAGGACGT CCTCTCTCCA TTCTCTCCAT CACATCCAGC CCCACCCTCC
    GACCCTTCCC ACCAGACAAA CCCAGACCCA ACTTGGGATA TCAAAAGGGA ACACGACGTT
    AGGAAACCAA AGGAGCTTTC AGCCGGCAGC CAGAAGAAGC AGCGCCTTGG GGAGCAGAGC
    GAGCGCTCAG CCAGCCCGCA AAGGGCCGTG CAACCAGGGC TCGAGGAGGC GCCTGGCGGC
    CAGGGGCAGC CTGAGGCCGG CAGGCCCTCC TCGGAGACCA GAGCGCCTGG GCCCGCACTG
    GCCGAAGCGG CGCGGGCTGG CAACAAGGAG GGGCTGCAGG CCCCGGTGGG CTCCGAGCTC
    GGCCGGCACG GAGGCCCCGG CGCGGCACCT GCGTTCGTGG GCCCGGGCCG CGGCGGGGGC
    GCACGGGAGG TCGAGGGCCG GGCCGGGGCG CGGGCGGGGT CCAGGCAGGG CTCGCGGCCC
    CCCGGGGGCG CCCCTGCGCG CAAGGCCGCC GTGGCGCCCG GGCCGTGGAA GGTGCCGGGC
    TCCGACAAGC TGCCGGGCGT CCTCAAACCC GGCGCCTCAG CCCCGGGGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89704
    Clone ID Related Accession (Same CDS sequence) XM_015468987.1 , XM_015463003.1
    Accession Version XM_015463003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCAACAAGTG
    CCACCAAGGA CCAGTTTTCG AATGGATTCT TCTGGTCCAG ATTATAAGGA GCTGGATGTT
    CACCTTCGCA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
    CAGCCTATTT TGATTGGAGC CGTCATCGCC ATGGGCTCAG CTGACAGAGA TGGCCGCCTA
    CACCCAGGAG ATGAGCTCGT CTATGTGGAT GGAATTCCAG TGGCTGGAAA GACCCACCGC
    TATGTCATTG ACCTTATGCA CCATGCAGCC CGGAATGGGC AGGTCAACCT CACTGTGAGA
    AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAATG GGAGGAGTCC AGGCTCCGTG
    TCGACCCACC ACAGTTCTCC TCGCAGTGAC TACGCAACCT ACACCAACAC CTACAGCAAC
    AGTAACCATG CCGTCCCCAC CAGCAACGCC TCTCCCCCCG AAGGCTTCGC CTCCCACAGC
    CTGCAGACCA GCGACGTGGT CATTCACCGC AAAGAGAACG AGGGTTTCGG CTTTGTCATC
    ATCAGCTCCC TGAACAGGCC AGAGTCTGGA TCCACTATAA CTGTGCCACA TAAAATTGGA
    CGCATCATTG ATGGGAGTCC CGCAGATCGC TGTGCAAAGC TAAAAGTGGG AGACCGGATC
    TTAGCGGTGA ACGGCCAGTC TATCATCAAC ATGCCTCATG CAGACATCGT GAAGCTCATC
    AAGGATGCGG GTCTTAGCGT CACCCTTCGC ATCATCCCTC AGGAGGAACT CAACAGCCCC
    GCTTCAGCAC CCAGCTCAGA GAAGCAGAGC CCCCTGGCCC AGCAGCACAG CCCCCTGGCC
    CAACAGAGTC CTCTGGCCCA GCCCAGCCCG GCCACGCCCA ACAGTCCCAT CGCCCAGCCA
    GCTCCGCCTC AGCCTCTTCA GCCTCAAGGA CATGAAAATA GTTACAGGTC AGAAGTAAAA
    GCGAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACGGACTA CAGGCAGCCT
    CCACTGGACT ACAGGCAACC CCCAGGAGGG GACTACCCGC AGCCCCCACC CTTGGACTAC
    AGGCAGCCTC CGCTACTGGA CTACAGGCAG CACTCCCCAG ACACCAGGCA GTTCCCTCTG
    TCAGACTACA GGCAGCCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
    AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAGTACA AAATGGATTT GTATGTGCTG
    AGATTAGCAG AAGATGGGCC AGCAATAAGG AATGGAAGGA TGAGGGTAGG AGATCAGATC
    ATTGAAATCA ATGGGGAGAG CACAAGAGAC ATGACCCATG CCAGAGCAAT AGAGCTCATC
    AAATCAGGCG GAAGGAGAGT GAGGCTACTG CTGAAGCGTG GGACGGGACA GGTCCCAGAG
    TATGACGAAC CCGGCTCCTG GAGTGCTCCC GCTGCCGCCT CCCCAGGTCT GCCGGAAGTA
    GGCGTCTCCC TGGAGGACGT CCTCTCTCCA TTCTCTCCAT CACATCCAGC CCCACCCTCC
    GACCCTTCCC ACCAGACAAA CCCAGACCCA ACTTGGGATA TCAAAAGGGA ACACGACGTT
    AGGAAACCAA AGGAGCTTTC AGCCGGCAGC CAGAAGAAGC AGCGCCTTGG GGAGCAGAGC
    GAGCGCTCAG CCAGCCCGCA AAGGGCCGTG CAACCAGGGC TCGAGGAGGC GCCTGGCGGC
    CAGGGGCAGC CTGAGGCCGG CAGGCCCTCC TCGGAGACCA GAGCGCCTGG GCCCGCACTG
    GCCGAAGCGG CGCGGGCTGG CAACAAGGAG GGGCTGCAGG CCCCGGTGGG CTCCGAGCTC
    GGCCGGCACG GAGGCCCCGG CGCGGCACCT GCGTTCGTGG GCCCGGGCCG CGGCGGGGGC
    GCACGGGAGG TCGAGGGCCG GGCCGGGGCG CGGGCGGGGT CCAGGCAGGG CTCGCGGCCC
    CCCGGGGGCG CCCCTGCGCG CAAGGCCGCC GTGGCGCCCG GGCCGTGGAA GGTGCCGGGC
    TCCGACAAGC TGCCGGGCGT CCTCAAACCC GGCGCCTCAG CCCCGGGGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318489.1
    CDS920..4933
    Translation

    Target ORF information:

    RefSeq Version XM_015463003.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCAACAAGTG
    CCACCAAGGA CCAGTTTTCG AATGGATTCT TCTGGTCCAG ATTATAAGGA GCTGGATGTT
    CACCTTCGCA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
    CAGCCTATTT TGATTGGAGC CGTCATCGCC ATGGGCTCAG CTGACAGAGA TGGCCGCCTA
    CACCCAGGAG ATGAGCTCGT CTATGTGGAT GGAATTCCAG TGGCTGGAAA GACCCACCGC
    TATGTCATTG ACCTTATGCA CCATGCAGCC CGGAATGGGC AGGTCAACCT CACTGTGAGA
    AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAATG GGAGGAGTCC AGGCTCCGTG
    TCGACCCACC ACAGTTCTCC TCGCAGTGAC TACGCAACCT ACACCAACAC CTACAGCAAC
    AGTAACCATG CCGTCCCCAC CAGCAACGCC TCTCCCCCCG AAGGCTTCGC CTCCCACAGC
    CTGCAGACCA GCGACGTGGT CATTCACCGC AAAGAGAACG AGGGTTTCGG CTTTGTCATC
    ATCAGCTCCC TGAACAGGCC AGAGTCTGGA TCCACTATAA CTGTGCCACA TAAAATTGGA
    CGCATCATTG ATGGGAGTCC CGCAGATCGC TGTGCAAAGC TAAAAGTGGG AGACCGGATC
    TTAGCGGTGA ACGGCCAGTC TATCATCAAC ATGCCTCATG CAGACATCGT GAAGCTCATC
    AAGGATGCGG GTCTTAGCGT CACCCTTCGC ATCATCCCTC AGGAGGAACT CAACAGCCCC
    GCTTCAGCAC CCAGCTCAGA GAAGCAGAGC CCCCTGGCCC AGCAGCACAG CCCCCTGGCC
    CAACAGAGTC CTCTGGCCCA GCCCAGCCCG GCCACGCCCA ACAGTCCCAT CGCCCAGCCA
    GCTCCGCCTC AGCCTCTTCA GCCTCAAGGA CATGAAAATA GTTACAGGTC AGAAGTAAAA
    GCGAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACGGACTA CAGGCAGCCT
    CCACTGGACT ACAGGCAACC CCCAGGAGGG GACTACCCGC AGCCCCCACC CTTGGACTAC
    AGGCAGCCTC CGCTACTGGA CTACAGGCAG CACTCCCCAG ACACCAGGCA GTTCCCTCTG
    TCAGACTACA GGCAGCCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
    AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAGTACA AAATGGATTT GTATGTGCTG
    AGATTAGCAG AAGATGGGCC AGCAATAAGG AATGGAAGGA TGAGGGTAGG AGATCAGATC
    ATTGAAATCA ATGGGGAGAG CACAAGAGAC ATGACCCATG CCAGAGCAAT AGAGCTCATC
    AAATCAGGCG GAAGGAGAGT GAGGCTACTG CTGAAGCGTG GGACGGGACA GGTCCCAGAG
    TATGACGAAC CCGGCTCCTG GAGTGCTCCC GCTGCCGCCT CCCCAGGTCT GCCGGAAGTA
    GGCGTCTCCC TGGAGGACGT CCTCTCTCCA TTCTCTCCAT CACATCCAGC CCCACCCTCC
    GACCCTTCCC ACCAGACAAA CCCAGACCCA ACTTGGGATA TCAAAAGGGA ACACGACGTT
    AGGAAACCAA AGGAGCTTTC AGCCGGCAGC CAGAAGAAGC AGCGCCTTGG GGAGCAGAGC
    GAGCGCTCAG CCAGCCCGCA AAGGGCCGTG CAACCAGGGC TCGAGGAGGC GCCTGGCGGC
    CAGGGGCAGC CTGAGGCCGG CAGGCCCTCC TCGGAGACCA GAGCGCCTGG GCCCGCACTG
    GCCGAAGCGG CGCGGGCTGG CAACAAGGAG GGGCTGCAGG CCCCGGTGGG CTCCGAGCTC
    GGCCGGCACG GAGGCCCCGG CGCGGCACCT GCGTTCGTGG GCCCGGGCCG CGGCGGGGGC
    GCACGGGAGG TCGAGGGCCG GGCCGGGGCG CGGGCGGGGT CCAGGCAGGG CTCGCGGCCC
    CCCGGGGGCG CCCCTGCGCG CAAGGCCGCC GTGGCGCCCG GGCCGTGGAA GGTGCCGGGC
    TCCGACAAGC TGCCGGGCGT CCTCAAACCC GGCGCCTCAG CCCCGGGGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89705
    Clone ID Related Accession (Same CDS sequence) XM_015463004.1 , XM_015468988.1
    Accession Version XM_015463004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGAAAAG CCACTCTTTA CCCGGGATGC CTCCCAATTG
    AAGGGAACGT TTCTCAGCAC CACACTCAAA AAGAGCAACA TGGGGTTTGG ATTTACCATT
    ATTGGCGGAG ATGAGCCTGA CGAGTTTCTG CAGGTGAAAA GTGTGATTCC CGACGGGCCT
    GCAGCACAAG ACGGGAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
    GTCCTTGGAC ACACTCATGC AGATGTAGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
    AGTGTCAACT TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGATCCTGCT
    AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
    AGACATAACT ATGAAACATA TTTGGAATAC ATTTCTCGGA CCTCACAGTC AGTTCCGGAT
    ATAACAGATC GGCCGCCTCA TACTTTGCAC TCAATGCCAG CTGATGGTCA GCTAGATGGC
    ACGTATCCAC CACCTGTCCA TGACGACAAT GTATCTATGG CTTCATCTGG GGCCACCCAA
    GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GTTTTGGCTT CACCATTGCA
    GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
    TGTGAAGGCG ACCTCATTAT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACG
    GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGCG AGACTTCTTT GATTATTCAT
    CGAGGAGGTT TCTTTTCTCC ATGGAAAACC CCAAAGCCTA TAATGGATCG ATGGGAGAAC
    CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CACAGAACCT GCCCTTCCCA
    CCCGCCCTTC ACAGGAGTTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC AAGGAAGCCT
    GACCCATATG AGCTCTACGA GAAATCGAGA GCCATTTATG AAAGTAGGCA ACAAGTGCCA
    CCAAGGACCA GTTTTCGAAT GGATTCTTCT GGTCCAGATT ATAAGGAGCT GGATGTTCAC
    CTTCGCAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
    CCTATTTTGA TTGGAGCCGT CATCGCCATG GGCTCAGCTG ACAGAGATGG CCGCCTACAC
    CCAGGAGATG AGCTCGTCTA TGTGGATGGA ATTCCAGTGG CTGGAAAGAC CCACCGCTAT
    GTCATTGACC TTATGCACCA TGCAGCCCGG AATGGGCAGG TCAACCTCAC TGTGAGAAGA
    AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAATGGGA GGAGTCCAGG CTCCGTGTCG
    ACCCACCACA GTTCTCCTCG CAGTGACTAC GCAACCTACA CCAACACCTA CAGCAACAGT
    AACCATGCCG TCCCCACCAG CAACGCCTCT CCCCCCGAAG GCTTCGCCTC CCACAGCCTG
    CAGACCAGCG ACGTGGTCAT TCACCGCAAA GAGAACGAGG GTTTCGGCTT TGTCATCATC
    AGCTCCCTGA ACAGGCCAGA GTCTGGATCC ACTATAACTG TGCCACATAA AATTGGACGC
    ATCATTGATG GGAGTCCCGC AGATCGCTGT GCAAAGCTAA AAGTGGGAGA CCGGATCTTA
    GCGGTGAACG GCCAGTCTAT CATCAACATG CCTCATGCAG ACATCGTGAA GCTCATCAAG
    GATGCGGGTC TTAGCGTCAC CCTTCGCATC ATCCCTCAGG AGGAACTCAA CAGCCCCGCT
    TCAGCACCCA GCTCAGAGAA GCAGAGCCCC CTGGCCCAGC AGCACAGCCC CCTGGCCCAA
    CAGAGTCCTC TGGCCCAGCC CAGCCCGGCC ACGCCCAACA GTCCCATCGC CCAGCCAGCT
    CCGCCTCAGC CTCTTCAGCC TCAAGGACAT GAAAATAGTT ACAGGTCAGA AGTAAAAGCG
    AGGCAAGATG TGAAACCAGA CATCCGACAG CCTCCATTCA CGGACTACAG GCAGCCTCCA
    CTGGACTACA GGCAACCCCC AGGAGGGGAC TACCCGCAGC CCCCACCCTT GGACTACAGG
    CAGCCTCCGC TACTGGACTA CAGGCAGCAC TCCCCAGACA CCAGGCAGTT CCCTCTGTCA
    GACTACAGGC AGCCCCAGGA TTTTGATTAT TTCACTGTGG ACATGGAGAA AGGAGCCAAA
    GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAGTACAAAA TGGATTTGTA TGTGCTGAGA
    TTAGCAGAAG ATGGGCCAGC AATAAGGAAT GGAAGGATGA GGGTAGGAGA TCAGATCATT
    GAAATCAATG GGGAGAGCAC AAGAGACATG ACCCATGCCA GAGCAATAGA GCTCATCAAA
    TCAGGCGGAA GGAGAGTGAG GCTACTGCTG AAGCGTGGGA CGGGACAGGT CCCAGAGTAT
    GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC CAGGTCTGCC GGAAGTAGGC
    GTCTCCCTGG AGGACGTCCT CTCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
    CCTTCCCACC AGACAAACCC AGACCCAACT TGGGATATCA AAAGGGAACA CGACGTTAGG
    AAACCAAAGG AGCTTTCAGC CGGCAGCCAG AAGAAGCAGC GCCTTGGGGA GCAGAGCGAG
    CGCTCAGCCA GCCCGCAAAG GGCCGTGCAA CCAGGGCTCG AGGAGGCGCC TGGCGGCCAG
    GGGCAGCCTG AGGCCGGCAG GCCCTCCTCG GAGACCAGAG CGCCTGGGCC CGCACTGGCC
    GAAGCGGCGC GGGCTGGCAA CAAGGAGGGG CTGCAGGCCC CGGTGGGCTC CGAGCTCGGC
    CGGCACGGAG GCCCCGGCGC GGCACCTGCG TTCGTGGGCC CGGGCCGCGG CGGGGGCGCA
    CGGGAGGTCG AGGGCCGGGC CGGGGCGCGG GCGGGGTCCA GGCAGGGCTC GCGGCCCCCC
    GGGGGCGCCC CTGCGCGCAA GGCCGCCGTG GCGCCCGGGC CGTGGAAGGT GCCGGGCTCC
    GACAAGCTGC CGGGCGTCCT CAAACCCGGC GCCTCAGCCC CGGGGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318490.1
    CDS919..4929
    Translation

    Target ORF information:

    RefSeq Version XM_015463004.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGAAAAG CCACTCTTTA CCCGGGATGC CTCCCAATTG
    AAGGGAACGT TTCTCAGCAC CACACTCAAA AAGAGCAACA TGGGGTTTGG ATTTACCATT
    ATTGGCGGAG ATGAGCCTGA CGAGTTTCTG CAGGTGAAAA GTGTGATTCC CGACGGGCCT
    GCAGCACAAG ACGGGAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
    GTCCTTGGAC ACACTCATGC AGATGTAGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
    AGTGTCAACT TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGATCCTGCT
    AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
    AGACATAACT ATGAAACATA TTTGGAATAC ATTTCTCGGA CCTCACAGTC AGTTCCGGAT
    ATAACAGATC GGCCGCCTCA TACTTTGCAC TCAATGCCAG CTGATGGTCA GCTAGATGGC
    ACGTATCCAC CACCTGTCCA TGACGACAAT GTATCTATGG CTTCATCTGG GGCCACCCAA
    GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GTTTTGGCTT CACCATTGCA
    GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
    TGTGAAGGCG ACCTCATTAT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACG
    GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGCG AGACTTCTTT GATTATTCAT
    CGAGGAGGTT TCTTTTCTCC ATGGAAAACC CCAAAGCCTA TAATGGATCG ATGGGAGAAC
    CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CACAGAACCT GCCCTTCCCA
    CCCGCCCTTC ACAGGAGTTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC AAGGAAGCCT
    GACCCATATG AGCTCTACGA GAAATCGAGA GCCATTTATG AAAGTAGGCA ACAAGTGCCA
    CCAAGGACCA GTTTTCGAAT GGATTCTTCT GGTCCAGATT ATAAGGAGCT GGATGTTCAC
    CTTCGCAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
    CCTATTTTGA TTGGAGCCGT CATCGCCATG GGCTCAGCTG ACAGAGATGG CCGCCTACAC
    CCAGGAGATG AGCTCGTCTA TGTGGATGGA ATTCCAGTGG CTGGAAAGAC CCACCGCTAT
    GTCATTGACC TTATGCACCA TGCAGCCCGG AATGGGCAGG TCAACCTCAC TGTGAGAAGA
    AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAATGGGA GGAGTCCAGG CTCCGTGTCG
    ACCCACCACA GTTCTCCTCG CAGTGACTAC GCAACCTACA CCAACACCTA CAGCAACAGT
    AACCATGCCG TCCCCACCAG CAACGCCTCT CCCCCCGAAG GCTTCGCCTC CCACAGCCTG
    CAGACCAGCG ACGTGGTCAT TCACCGCAAA GAGAACGAGG GTTTCGGCTT TGTCATCATC
    AGCTCCCTGA ACAGGCCAGA GTCTGGATCC ACTATAACTG TGCCACATAA AATTGGACGC
    ATCATTGATG GGAGTCCCGC AGATCGCTGT GCAAAGCTAA AAGTGGGAGA CCGGATCTTA
    GCGGTGAACG GCCAGTCTAT CATCAACATG CCTCATGCAG ACATCGTGAA GCTCATCAAG
    GATGCGGGTC TTAGCGTCAC CCTTCGCATC ATCCCTCAGG AGGAACTCAA CAGCCCCGCT
    TCAGCACCCA GCTCAGAGAA GCAGAGCCCC CTGGCCCAGC AGCACAGCCC CCTGGCCCAA
    CAGAGTCCTC TGGCCCAGCC CAGCCCGGCC ACGCCCAACA GTCCCATCGC CCAGCCAGCT
    CCGCCTCAGC CTCTTCAGCC TCAAGGACAT GAAAATAGTT ACAGGTCAGA AGTAAAAGCG
    AGGCAAGATG TGAAACCAGA CATCCGACAG CCTCCATTCA CGGACTACAG GCAGCCTCCA
    CTGGACTACA GGCAACCCCC AGGAGGGGAC TACCCGCAGC CCCCACCCTT GGACTACAGG
    CAGCCTCCGC TACTGGACTA CAGGCAGCAC TCCCCAGACA CCAGGCAGTT CCCTCTGTCA
    GACTACAGGC AGCCCCAGGA TTTTGATTAT TTCACTGTGG ACATGGAGAA AGGAGCCAAA
    GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAGTACAAAA TGGATTTGTA TGTGCTGAGA
    TTAGCAGAAG ATGGGCCAGC AATAAGGAAT GGAAGGATGA GGGTAGGAGA TCAGATCATT
    GAAATCAATG GGGAGAGCAC AAGAGACATG ACCCATGCCA GAGCAATAGA GCTCATCAAA
    TCAGGCGGAA GGAGAGTGAG GCTACTGCTG AAGCGTGGGA CGGGACAGGT CCCAGAGTAT
    GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC CAGGTCTGCC GGAAGTAGGC
    GTCTCCCTGG AGGACGTCCT CTCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
    CCTTCCCACC AGACAAACCC AGACCCAACT TGGGATATCA AAAGGGAACA CGACGTTAGG
    AAACCAAAGG AGCTTTCAGC CGGCAGCCAG AAGAAGCAGC GCCTTGGGGA GCAGAGCGAG
    CGCTCAGCCA GCCCGCAAAG GGCCGTGCAA CCAGGGCTCG AGGAGGCGCC TGGCGGCCAG
    GGGCAGCCTG AGGCCGGCAG GCCCTCCTCG GAGACCAGAG CGCCTGGGCC CGCACTGGCC
    GAAGCGGCGC GGGCTGGCAA CAAGGAGGGG CTGCAGGCCC CGGTGGGCTC CGAGCTCGGC
    CGGCACGGAG GCCCCGGCGC GGCACCTGCG TTCGTGGGCC CGGGCCGCGG CGGGGGCGCA
    CGGGAGGTCG AGGGCCGGGC CGGGGCGCGG GCGGGGTCCA GGCAGGGCTC GCGGCCCCCC
    GGGGGCGCCC CTGCGCGCAA GGCCGCCGTG GCGCCCGGGC CGTGGAAGGT GCCGGGCTCC
    GACAAGCTGC CGGGCGTCCT CAAACCCGGC GCCTCAGCCC CGGGGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89705
    Clone ID Related Accession (Same CDS sequence) XM_015463004.1 , XM_015468988.1
    Accession Version XM_015468988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGAAAAG CCACTCTTTA CCCGGGATGC CTCCCAATTG
    AAGGGAACGT TTCTCAGCAC CACACTCAAA AAGAGCAACA TGGGGTTTGG ATTTACCATT
    ATTGGCGGAG ATGAGCCTGA CGAGTTTCTG CAGGTGAAAA GTGTGATTCC CGACGGGCCT
    GCAGCACAAG ACGGGAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
    GTCCTTGGAC ACACTCATGC AGATGTAGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
    AGTGTCAACT TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGATCCTGCT
    AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
    AGACATAACT ATGAAACATA TTTGGAATAC ATTTCTCGGA CCTCACAGTC AGTTCCGGAT
    ATAACAGATC GGCCGCCTCA TACTTTGCAC TCAATGCCAG CTGATGGTCA GCTAGATGGC
    ACGTATCCAC CACCTGTCCA TGACGACAAT GTATCTATGG CTTCATCTGG GGCCACCCAA
    GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GTTTTGGCTT CACCATTGCA
    GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
    TGTGAAGGCG ACCTCATTAT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACG
    GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGCG AGACTTCTTT GATTATTCAT
    CGAGGAGGTT TCTTTTCTCC ATGGAAAACC CCAAAGCCTA TAATGGATCG ATGGGAGAAC
    CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CACAGAACCT GCCCTTCCCA
    CCCGCCCTTC ACAGGAGTTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC AAGGAAGCCT
    GACCCATATG AGCTCTACGA GAAATCGAGA GCCATTTATG AAAGTAGGCA ACAAGTGCCA
    CCAAGGACCA GTTTTCGAAT GGATTCTTCT GGTCCAGATT ATAAGGAGCT GGATGTTCAC
    CTTCGCAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
    CCTATTTTGA TTGGAGCCGT CATCGCCATG GGCTCAGCTG ACAGAGATGG CCGCCTACAC
    CCAGGAGATG AGCTCGTCTA TGTGGATGGA ATTCCAGTGG CTGGAAAGAC CCACCGCTAT
    GTCATTGACC TTATGCACCA TGCAGCCCGG AATGGGCAGG TCAACCTCAC TGTGAGAAGA
    AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAATGGGA GGAGTCCAGG CTCCGTGTCG
    ACCCACCACA GTTCTCCTCG CAGTGACTAC GCAACCTACA CCAACACCTA CAGCAACAGT
    AACCATGCCG TCCCCACCAG CAACGCCTCT CCCCCCGAAG GCTTCGCCTC CCACAGCCTG
    CAGACCAGCG ACGTGGTCAT TCACCGCAAA GAGAACGAGG GTTTCGGCTT TGTCATCATC
    AGCTCCCTGA ACAGGCCAGA GTCTGGATCC ACTATAACTG TGCCACATAA AATTGGACGC
    ATCATTGATG GGAGTCCCGC AGATCGCTGT GCAAAGCTAA AAGTGGGAGA CCGGATCTTA
    GCGGTGAACG GCCAGTCTAT CATCAACATG CCTCATGCAG ACATCGTGAA GCTCATCAAG
    GATGCGGGTC TTAGCGTCAC CCTTCGCATC ATCCCTCAGG AGGAACTCAA CAGCCCCGCT
    TCAGCACCCA GCTCAGAGAA GCAGAGCCCC CTGGCCCAGC AGCACAGCCC CCTGGCCCAA
    CAGAGTCCTC TGGCCCAGCC CAGCCCGGCC ACGCCCAACA GTCCCATCGC CCAGCCAGCT
    CCGCCTCAGC CTCTTCAGCC TCAAGGACAT GAAAATAGTT ACAGGTCAGA AGTAAAAGCG
    AGGCAAGATG TGAAACCAGA CATCCGACAG CCTCCATTCA CGGACTACAG GCAGCCTCCA
    CTGGACTACA GGCAACCCCC AGGAGGGGAC TACCCGCAGC CCCCACCCTT GGACTACAGG
    CAGCCTCCGC TACTGGACTA CAGGCAGCAC TCCCCAGACA CCAGGCAGTT CCCTCTGTCA
    GACTACAGGC AGCCCCAGGA TTTTGATTAT TTCACTGTGG ACATGGAGAA AGGAGCCAAA
    GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAGTACAAAA TGGATTTGTA TGTGCTGAGA
    TTAGCAGAAG ATGGGCCAGC AATAAGGAAT GGAAGGATGA GGGTAGGAGA TCAGATCATT
    GAAATCAATG GGGAGAGCAC AAGAGACATG ACCCATGCCA GAGCAATAGA GCTCATCAAA
    TCAGGCGGAA GGAGAGTGAG GCTACTGCTG AAGCGTGGGA CGGGACAGGT CCCAGAGTAT
    GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC CAGGTCTGCC GGAAGTAGGC
    GTCTCCCTGG AGGACGTCCT CTCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
    CCTTCCCACC AGACAAACCC AGACCCAACT TGGGATATCA AAAGGGAACA CGACGTTAGG
    AAACCAAAGG AGCTTTCAGC CGGCAGCCAG AAGAAGCAGC GCCTTGGGGA GCAGAGCGAG
    CGCTCAGCCA GCCCGCAAAG GGCCGTGCAA CCAGGGCTCG AGGAGGCGCC TGGCGGCCAG
    GGGCAGCCTG AGGCCGGCAG GCCCTCCTCG GAGACCAGAG CGCCTGGGCC CGCACTGGCC
    GAAGCGGCGC GGGCTGGCAA CAAGGAGGGG CTGCAGGCCC CGGTGGGCTC CGAGCTCGGC
    CGGCACGGAG GCCCCGGCGC GGCACCTGCG TTCGTGGGCC CGGGCCGCGG CGGGGGCGCA
    CGGGAGGTCG AGGGCCGGGC CGGGGCGCGG GCGGGGTCCA GGCAGGGCTC GCGGCCCCCC
    GGGGGCGCCC CTGCGCGCAA GGCCGCCGTG GCGCCCGGGC CGTGGAAGGT GCCGGGCTCC
    GACAAGCTGC CGGGCGTCCT CAAACCCGGC GCCTCAGCCC CGGGGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324474.1
    CDS919..4929
    Translation

    Target ORF information:

    RefSeq Version XM_015468988.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGAAAAG CCACTCTTTA CCCGGGATGC CTCCCAATTG
    AAGGGAACGT TTCTCAGCAC CACACTCAAA AAGAGCAACA TGGGGTTTGG ATTTACCATT
    ATTGGCGGAG ATGAGCCTGA CGAGTTTCTG CAGGTGAAAA GTGTGATTCC CGACGGGCCT
    GCAGCACAAG ACGGGAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
    GTCCTTGGAC ACACTCATGC AGATGTAGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
    AGTGTCAACT TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGATCCTGCT
    AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
    AGACATAACT ATGAAACATA TTTGGAATAC ATTTCTCGGA CCTCACAGTC AGTTCCGGAT
    ATAACAGATC GGCCGCCTCA TACTTTGCAC TCAATGCCAG CTGATGGTCA GCTAGATGGC
    ACGTATCCAC CACCTGTCCA TGACGACAAT GTATCTATGG CTTCATCTGG GGCCACCCAA
    GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GTTTTGGCTT CACCATTGCA
    GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
    TGTGAAGGCG ACCTCATTAT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACG
    GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGCG AGACTTCTTT GATTATTCAT
    CGAGGAGGTT TCTTTTCTCC ATGGAAAACC CCAAAGCCTA TAATGGATCG ATGGGAGAAC
    CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CACAGAACCT GCCCTTCCCA
    CCCGCCCTTC ACAGGAGTTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC AAGGAAGCCT
    GACCCATATG AGCTCTACGA GAAATCGAGA GCCATTTATG AAAGTAGGCA ACAAGTGCCA
    CCAAGGACCA GTTTTCGAAT GGATTCTTCT GGTCCAGATT ATAAGGAGCT GGATGTTCAC
    CTTCGCAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
    CCTATTTTGA TTGGAGCCGT CATCGCCATG GGCTCAGCTG ACAGAGATGG CCGCCTACAC
    CCAGGAGATG AGCTCGTCTA TGTGGATGGA ATTCCAGTGG CTGGAAAGAC CCACCGCTAT
    GTCATTGACC TTATGCACCA TGCAGCCCGG AATGGGCAGG TCAACCTCAC TGTGAGAAGA
    AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAATGGGA GGAGTCCAGG CTCCGTGTCG
    ACCCACCACA GTTCTCCTCG CAGTGACTAC GCAACCTACA CCAACACCTA CAGCAACAGT
    AACCATGCCG TCCCCACCAG CAACGCCTCT CCCCCCGAAG GCTTCGCCTC CCACAGCCTG
    CAGACCAGCG ACGTGGTCAT TCACCGCAAA GAGAACGAGG GTTTCGGCTT TGTCATCATC
    AGCTCCCTGA ACAGGCCAGA GTCTGGATCC ACTATAACTG TGCCACATAA AATTGGACGC
    ATCATTGATG GGAGTCCCGC AGATCGCTGT GCAAAGCTAA AAGTGGGAGA CCGGATCTTA
    GCGGTGAACG GCCAGTCTAT CATCAACATG CCTCATGCAG ACATCGTGAA GCTCATCAAG
    GATGCGGGTC TTAGCGTCAC CCTTCGCATC ATCCCTCAGG AGGAACTCAA CAGCCCCGCT
    TCAGCACCCA GCTCAGAGAA GCAGAGCCCC CTGGCCCAGC AGCACAGCCC CCTGGCCCAA
    CAGAGTCCTC TGGCCCAGCC CAGCCCGGCC ACGCCCAACA GTCCCATCGC CCAGCCAGCT
    CCGCCTCAGC CTCTTCAGCC TCAAGGACAT GAAAATAGTT ACAGGTCAGA AGTAAAAGCG
    AGGCAAGATG TGAAACCAGA CATCCGACAG CCTCCATTCA CGGACTACAG GCAGCCTCCA
    CTGGACTACA GGCAACCCCC AGGAGGGGAC TACCCGCAGC CCCCACCCTT GGACTACAGG
    CAGCCTCCGC TACTGGACTA CAGGCAGCAC TCCCCAGACA CCAGGCAGTT CCCTCTGTCA
    GACTACAGGC AGCCCCAGGA TTTTGATTAT TTCACTGTGG ACATGGAGAA AGGAGCCAAA
    GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAGTACAAAA TGGATTTGTA TGTGCTGAGA
    TTAGCAGAAG ATGGGCCAGC AATAAGGAAT GGAAGGATGA GGGTAGGAGA TCAGATCATT
    GAAATCAATG GGGAGAGCAC AAGAGACATG ACCCATGCCA GAGCAATAGA GCTCATCAAA
    TCAGGCGGAA GGAGAGTGAG GCTACTGCTG AAGCGTGGGA CGGGACAGGT CCCAGAGTAT
    GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC CAGGTCTGCC GGAAGTAGGC
    GTCTCCCTGG AGGACGTCCT CTCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
    CCTTCCCACC AGACAAACCC AGACCCAACT TGGGATATCA AAAGGGAACA CGACGTTAGG
    AAACCAAAGG AGCTTTCAGC CGGCAGCCAG AAGAAGCAGC GCCTTGGGGA GCAGAGCGAG
    CGCTCAGCCA GCCCGCAAAG GGCCGTGCAA CCAGGGCTCG AGGAGGCGCC TGGCGGCCAG
    GGGCAGCCTG AGGCCGGCAG GCCCTCCTCG GAGACCAGAG CGCCTGGGCC CGCACTGGCC
    GAAGCGGCGC GGGCTGGCAA CAAGGAGGGG CTGCAGGCCC CGGTGGGCTC CGAGCTCGGC
    CGGCACGGAG GCCCCGGCGC GGCACCTGCG TTCGTGGGCC CGGGCCGCGG CGGGGGCGCA
    CGGGAGGTCG AGGGCCGGGC CGGGGCGCGG GCGGGGTCCA GGCAGGGCTC GCGGCCCCCC
    GGGGGCGCCC CTGCGCGCAA GGCCGCCGTG GCGCCCGGGC CGTGGAAGGT GCCGGGCTCC
    GACAAGCTGC CGGGCGTCCT CAAACCCGGC GCCTCAGCCC CGGGGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89706
    Clone ID Related Accession (Same CDS sequence) XM_015468989.1 , XM_015463005.1
    Accession Version XM_015468989.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGACAG CTCCTCATCG ACAGTACCTA GGCCCAAACC TGCCCGCTCC
    CTCGCCGCCC GGGAGCGGTC CCGCAGCGTG AGTGACCTGT CTTACTACGG GAGCCACCTC
    CCCGGGAGGC TCTGGCGCAT CGTAGAAAAG CCACTCTTTA CCCGGGATGC CTCCCAATTG
    AAGGGAACGT TTCTCAGCAC CACACTCAAA AAGAGCAACA TGGGGTTTGG ATTTACCATT
    ATTGGCGGAG ATGAGCCTGA CGAGTTTCTG CAGGTGAAAA GTGTGATTCC CGACGGGCCT
    GCAGCACAAG ACGGGAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
    GTCCTTGGAC ACACTCATGC AGATGTAGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
    AGTGTCAACT TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGATCCTGCT
    AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
    AGACATAACT ATGAAACATA TTTGGAATAC ATTTCTCGGA CCTCACAGTC AGTTCCGGAT
    ATAACAGATC GGCCGCCTCA TACTTTGCAC TCAATGCCAG CTGATGGTCA GCTAGATGGC
    ACGTATCCAC CACCTGTCCA TGACGACAAT GTATCTATGG CTTCATCTGG GGCCACCCAA
    GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GTTTTGGCTT CACCATTGCA
    GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
    TGTGAAGGCG ACCTCATTAT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACG
    GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGCG AGACTTCTTT GATTATTCAT
    CGAGGAGGTT TCTTTTCTCC ATGGAAAACC CCAAAGCCTA TAATGGATCG ATGGGAGAAC
    CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CACAGAACCT GCCCTTCCCA
    CCCGCCCTTC ACAGGAGTTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC AAGGAAGCCT
    GACCCATATG AGCTCTACGA GAAATCGAGA GCCATTTATG AAAGTAGGCA ACAAGTGCCA
    CCAAGGACCA GTTTTCGAAT GGATTCTTCT GGTCCAGATT ATAAGGAGCT GGATGTTCAC
    CTTCGCAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
    CCTATTTTGA TTGGAGCCGT CATCGCCATG GGCTCAGCTG ACAGAGATGG CCGCCTACAC
    CCAGGAGATG AGCTCGTCTA TGTGGATGGA ATTCCAGTGG CTGGAAAGAC CCACCGCTAT
    GTCATTGACC TTATGCACCA TGCAGCCCGG AATGGGCAGG TCAACCTCAC TGTGAGAAGA
    AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAATGGGA GGAGTCCAGG CTCCGTGTCG
    ACCCACCACA GTTCTCCTCG CAGTGACTAC GCAACCTACA CCAACACCTA CAGCAACAGT
    AACCATGCCG TCCCCACCAG CAACGCCTCT CCCCCCGAAG GCTTCGCCTC CCACAGCCTG
    CAGACCAGCG ACGTGGTCAT TCACCGCAAA GAGAACGAGG GTTTCGGCTT TGTCATCATC
    AGCTCCCTGA ACAGGCCAGA GTCTGGATCC ACTATAACTG TGCCACATAA AATTGGACGC
    ATCATTGATG GGAGTCCCGC AGATCGCTGT GCAAAGCTAA AAGTGGGAGA CCGGATCTTA
    GCGGTGAACG GCCAGTCTAT CATCAACATG CCTCATGCAG ACATCGTGAA GCTCATCAAG
    GATGCGGGTC TTAGCGTCAC CCTTCGCATC ATCCCTCAGG AGGAACTCAA CAGCCCCGCT
    TCAGCACCCA GCTCAGAGAA GCAGAGCCCC CTGGCCCAGC AGCACAGCCC CCTGGCCCAA
    CAGAGTCCTC TGGCCCAGCC CAGCCCGGCC ACGCCCAACA GTCCCATCGC CCAGCCAGCT
    CCGCCTCAGC CTCTTCAGCC TCAAGGACAT GAAAATAGTT ACAGGTCAGA AGTAAAAGCG
    AGGCAAGATG TGAAACCAGA CATCCGACAG CCTCCATTCA CGGACTACAG GCAGCCTCCA
    CTGGACTACA GGCAACCCCC AGGAGGGGAC TACCCGCAGC CCCCACCCTT GGACTACAGG
    CAGCCTCCGC TACTGGACTA CAGGCAGCAC TCCCCAGACA CCAGGCAGTT CCCTCTGTCA
    GACTACAGGC AGCCCCAGGA TTTTGATTAT TTCACTGTGG ACATGGAGAA AGGAGCCAAA
    GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAGTACAAAA TGGATTTGTA TGTGCTGAGA
    TTAGCAGAAG ATGGGCCAGC AATAAGGAAT GGAAGGATGA GGGTAGGAGA TCAGATCATT
    GAAATCAATG GGGAGAGCAC AAGAGACATG ACCCATGCCA GAGCAATAGA GCTCATCAAA
    TCAGGCGGAA GGAGAGTGAG GCTACTGCTG AAGCGTGGGA CGGGACAGGT CCCAGAGTAT
    GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC CAGGTCTGCC GGAAGTAGGC
    GTCTCCCTGG AGGACGTCCT CTCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
    CCTTCCCACC AGACAAACCC AGACCCAACT TGGGATATCA AAAGGGAACA CGACGTTAGG
    AAACCAAAGG AGCTTTCAGC CGGCAGCCAG AAGAAGCAGC GCCTTGGGGA GCAGAGCGAG
    CGCTCAGCCA GCCCGCAAAG GGCCGTGCAA CCAGGGCTCG AGGAGGCGCC TGGCGGCCAG
    GGGCAGCCTG AGGCCGGCAG GCCCTCCTCG GAGACCAGAG CGCCTGGGCC CGCACTGGCC
    GAAGCGGCGC GGGCTGGCAA CAAGGAGGGG CTGCAGGCCC CGGTGGGCTC CGAGCTCGGC
    CGGCACGGAG GCCCCGGCGC GGCACCTGCG TTCGTGGGCC CGGGCCGCGG CGGGGGCGCA
    CGGGAGGTCG AGGGCCGGGC CGGGGCGCGG GCGGGGTCCA GGCAGGGCTC GCGGCCCCCC
    GGGGGCGCCC CTGCGCGCAA GGCCGCCGTG GCGCCCGGGC CGTGGAAGGT GCCGGGCTCC
    GACAAGCTGC CGGGCGTCCT CAAACCCGGC GCCTCAGCCC CGGGGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324475.1
    CDS920..4930
    Translation

    Target ORF information:

    RefSeq Version XM_015468989.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGACAG CTCCTCATCG ACAGTACCTA GGCCCAAACC TGCCCGCTCC
    CTCGCCGCCC GGGAGCGGTC CCGCAGCGTG AGTGACCTGT CTTACTACGG GAGCCACCTC
    CCCGGGAGGC TCTGGCGCAT CGTAGAAAAG CCACTCTTTA CCCGGGATGC CTCCCAATTG
    AAGGGAACGT TTCTCAGCAC CACACTCAAA AAGAGCAACA TGGGGTTTGG ATTTACCATT
    ATTGGCGGAG ATGAGCCTGA CGAGTTTCTG CAGGTGAAAA GTGTGATTCC CGACGGGCCT
    GCAGCACAAG ACGGGAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
    GTCCTTGGAC ACACTCATGC AGATGTAGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
    AGTGTCAACT TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGATCCTGCT
    AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
    AGACATAACT ATGAAACATA TTTGGAATAC ATTTCTCGGA CCTCACAGTC AGTTCCGGAT
    ATAACAGATC GGCCGCCTCA TACTTTGCAC TCAATGCCAG CTGATGGTCA GCTAGATGGC
    ACGTATCCAC CACCTGTCCA TGACGACAAT GTATCTATGG CTTCATCTGG GGCCACCCAA
    GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GTTTTGGCTT CACCATTGCA
    GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
    TGTGAAGGCG ACCTCATTAT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACG
    GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGCG AGACTTCTTT GATTATTCAT
    CGAGGAGGTT TCTTTTCTCC ATGGAAAACC CCAAAGCCTA TAATGGATCG ATGGGAGAAC
    CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CACAGAACCT GCCCTTCCCA
    CCCGCCCTTC ACAGGAGTTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC AAGGAAGCCT
    GACCCATATG AGCTCTACGA GAAATCGAGA GCCATTTATG AAAGTAGGCA ACAAGTGCCA
    CCAAGGACCA GTTTTCGAAT GGATTCTTCT GGTCCAGATT ATAAGGAGCT GGATGTTCAC
    CTTCGCAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
    CCTATTTTGA TTGGAGCCGT CATCGCCATG GGCTCAGCTG ACAGAGATGG CCGCCTACAC
    CCAGGAGATG AGCTCGTCTA TGTGGATGGA ATTCCAGTGG CTGGAAAGAC CCACCGCTAT
    GTCATTGACC TTATGCACCA TGCAGCCCGG AATGGGCAGG TCAACCTCAC TGTGAGAAGA
    AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAATGGGA GGAGTCCAGG CTCCGTGTCG
    ACCCACCACA GTTCTCCTCG CAGTGACTAC GCAACCTACA CCAACACCTA CAGCAACAGT
    AACCATGCCG TCCCCACCAG CAACGCCTCT CCCCCCGAAG GCTTCGCCTC CCACAGCCTG
    CAGACCAGCG ACGTGGTCAT TCACCGCAAA GAGAACGAGG GTTTCGGCTT TGTCATCATC
    AGCTCCCTGA ACAGGCCAGA GTCTGGATCC ACTATAACTG TGCCACATAA AATTGGACGC
    ATCATTGATG GGAGTCCCGC AGATCGCTGT GCAAAGCTAA AAGTGGGAGA CCGGATCTTA
    GCGGTGAACG GCCAGTCTAT CATCAACATG CCTCATGCAG ACATCGTGAA GCTCATCAAG
    GATGCGGGTC TTAGCGTCAC CCTTCGCATC ATCCCTCAGG AGGAACTCAA CAGCCCCGCT
    TCAGCACCCA GCTCAGAGAA GCAGAGCCCC CTGGCCCAGC AGCACAGCCC CCTGGCCCAA
    CAGAGTCCTC TGGCCCAGCC CAGCCCGGCC ACGCCCAACA GTCCCATCGC CCAGCCAGCT
    CCGCCTCAGC CTCTTCAGCC TCAAGGACAT GAAAATAGTT ACAGGTCAGA AGTAAAAGCG
    AGGCAAGATG TGAAACCAGA CATCCGACAG CCTCCATTCA CGGACTACAG GCAGCCTCCA
    CTGGACTACA GGCAACCCCC AGGAGGGGAC TACCCGCAGC CCCCACCCTT GGACTACAGG
    CAGCCTCCGC TACTGGACTA CAGGCAGCAC TCCCCAGACA CCAGGCAGTT CCCTCTGTCA
    GACTACAGGC AGCCCCAGGA TTTTGATTAT TTCACTGTGG ACATGGAGAA AGGAGCCAAA
    GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAGTACAAAA TGGATTTGTA TGTGCTGAGA
    TTAGCAGAAG ATGGGCCAGC AATAAGGAAT GGAAGGATGA GGGTAGGAGA TCAGATCATT
    GAAATCAATG GGGAGAGCAC AAGAGACATG ACCCATGCCA GAGCAATAGA GCTCATCAAA
    TCAGGCGGAA GGAGAGTGAG GCTACTGCTG AAGCGTGGGA CGGGACAGGT CCCAGAGTAT
    GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC CAGGTCTGCC GGAAGTAGGC
    GTCTCCCTGG AGGACGTCCT CTCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
    CCTTCCCACC AGACAAACCC AGACCCAACT TGGGATATCA AAAGGGAACA CGACGTTAGG
    AAACCAAAGG AGCTTTCAGC CGGCAGCCAG AAGAAGCAGC GCCTTGGGGA GCAGAGCGAG
    CGCTCAGCCA GCCCGCAAAG GGCCGTGCAA CCAGGGCTCG AGGAGGCGCC TGGCGGCCAG
    GGGCAGCCTG AGGCCGGCAG GCCCTCCTCG GAGACCAGAG CGCCTGGGCC CGCACTGGCC
    GAAGCGGCGC GGGCTGGCAA CAAGGAGGGG CTGCAGGCCC CGGTGGGCTC CGAGCTCGGC
    CGGCACGGAG GCCCCGGCGC GGCACCTGCG TTCGTGGGCC CGGGCCGCGG CGGGGGCGCA
    CGGGAGGTCG AGGGCCGGGC CGGGGCGCGG GCGGGGTCCA GGCAGGGCTC GCGGCCCCCC
    GGGGGCGCCC CTGCGCGCAA GGCCGCCGTG GCGCCCGGGC CGTGGAAGGT GCCGGGCTCC
    GACAAGCTGC CGGGCGTCCT CAAACCCGGC GCCTCAGCCC CGGGGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89706
    Clone ID Related Accession (Same CDS sequence) XM_015468989.1 , XM_015463005.1
    Accession Version XM_015463005.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGACAG CTCCTCATCG ACAGTACCTA GGCCCAAACC TGCCCGCTCC
    CTCGCCGCCC GGGAGCGGTC CCGCAGCGTG AGTGACCTGT CTTACTACGG GAGCCACCTC
    CCCGGGAGGC TCTGGCGCAT CGTAGAAAAG CCACTCTTTA CCCGGGATGC CTCCCAATTG
    AAGGGAACGT TTCTCAGCAC CACACTCAAA AAGAGCAACA TGGGGTTTGG ATTTACCATT
    ATTGGCGGAG ATGAGCCTGA CGAGTTTCTG CAGGTGAAAA GTGTGATTCC CGACGGGCCT
    GCAGCACAAG ACGGGAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
    GTCCTTGGAC ACACTCATGC AGATGTAGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
    AGTGTCAACT TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGATCCTGCT
    AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
    AGACATAACT ATGAAACATA TTTGGAATAC ATTTCTCGGA CCTCACAGTC AGTTCCGGAT
    ATAACAGATC GGCCGCCTCA TACTTTGCAC TCAATGCCAG CTGATGGTCA GCTAGATGGC
    ACGTATCCAC CACCTGTCCA TGACGACAAT GTATCTATGG CTTCATCTGG GGCCACCCAA
    GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GTTTTGGCTT CACCATTGCA
    GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
    TGTGAAGGCG ACCTCATTAT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACG
    GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGCG AGACTTCTTT GATTATTCAT
    CGAGGAGGTT TCTTTTCTCC ATGGAAAACC CCAAAGCCTA TAATGGATCG ATGGGAGAAC
    CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CACAGAACCT GCCCTTCCCA
    CCCGCCCTTC ACAGGAGTTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC AAGGAAGCCT
    GACCCATATG AGCTCTACGA GAAATCGAGA GCCATTTATG AAAGTAGGCA ACAAGTGCCA
    CCAAGGACCA GTTTTCGAAT GGATTCTTCT GGTCCAGATT ATAAGGAGCT GGATGTTCAC
    CTTCGCAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
    CCTATTTTGA TTGGAGCCGT CATCGCCATG GGCTCAGCTG ACAGAGATGG CCGCCTACAC
    CCAGGAGATG AGCTCGTCTA TGTGGATGGA ATTCCAGTGG CTGGAAAGAC CCACCGCTAT
    GTCATTGACC TTATGCACCA TGCAGCCCGG AATGGGCAGG TCAACCTCAC TGTGAGAAGA
    AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAATGGGA GGAGTCCAGG CTCCGTGTCG
    ACCCACCACA GTTCTCCTCG CAGTGACTAC GCAACCTACA CCAACACCTA CAGCAACAGT
    AACCATGCCG TCCCCACCAG CAACGCCTCT CCCCCCGAAG GCTTCGCCTC CCACAGCCTG
    CAGACCAGCG ACGTGGTCAT TCACCGCAAA GAGAACGAGG GTTTCGGCTT TGTCATCATC
    AGCTCCCTGA ACAGGCCAGA GTCTGGATCC ACTATAACTG TGCCACATAA AATTGGACGC
    ATCATTGATG GGAGTCCCGC AGATCGCTGT GCAAAGCTAA AAGTGGGAGA CCGGATCTTA
    GCGGTGAACG GCCAGTCTAT CATCAACATG CCTCATGCAG ACATCGTGAA GCTCATCAAG
    GATGCGGGTC TTAGCGTCAC CCTTCGCATC ATCCCTCAGG AGGAACTCAA CAGCCCCGCT
    TCAGCACCCA GCTCAGAGAA GCAGAGCCCC CTGGCCCAGC AGCACAGCCC CCTGGCCCAA
    CAGAGTCCTC TGGCCCAGCC CAGCCCGGCC ACGCCCAACA GTCCCATCGC CCAGCCAGCT
    CCGCCTCAGC CTCTTCAGCC TCAAGGACAT GAAAATAGTT ACAGGTCAGA AGTAAAAGCG
    AGGCAAGATG TGAAACCAGA CATCCGACAG CCTCCATTCA CGGACTACAG GCAGCCTCCA
    CTGGACTACA GGCAACCCCC AGGAGGGGAC TACCCGCAGC CCCCACCCTT GGACTACAGG
    CAGCCTCCGC TACTGGACTA CAGGCAGCAC TCCCCAGACA CCAGGCAGTT CCCTCTGTCA
    GACTACAGGC AGCCCCAGGA TTTTGATTAT TTCACTGTGG ACATGGAGAA AGGAGCCAAA
    GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAGTACAAAA TGGATTTGTA TGTGCTGAGA
    TTAGCAGAAG ATGGGCCAGC AATAAGGAAT GGAAGGATGA GGGTAGGAGA TCAGATCATT
    GAAATCAATG GGGAGAGCAC AAGAGACATG ACCCATGCCA GAGCAATAGA GCTCATCAAA
    TCAGGCGGAA GGAGAGTGAG GCTACTGCTG AAGCGTGGGA CGGGACAGGT CCCAGAGTAT
    GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC CAGGTCTGCC GGAAGTAGGC
    GTCTCCCTGG AGGACGTCCT CTCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
    CCTTCCCACC AGACAAACCC AGACCCAACT TGGGATATCA AAAGGGAACA CGACGTTAGG
    AAACCAAAGG AGCTTTCAGC CGGCAGCCAG AAGAAGCAGC GCCTTGGGGA GCAGAGCGAG
    CGCTCAGCCA GCCCGCAAAG GGCCGTGCAA CCAGGGCTCG AGGAGGCGCC TGGCGGCCAG
    GGGCAGCCTG AGGCCGGCAG GCCCTCCTCG GAGACCAGAG CGCCTGGGCC CGCACTGGCC
    GAAGCGGCGC GGGCTGGCAA CAAGGAGGGG CTGCAGGCCC CGGTGGGCTC CGAGCTCGGC
    CGGCACGGAG GCCCCGGCGC GGCACCTGCG TTCGTGGGCC CGGGCCGCGG CGGGGGCGCA
    CGGGAGGTCG AGGGCCGGGC CGGGGCGCGG GCGGGGTCCA GGCAGGGCTC GCGGCCCCCC
    GGGGGCGCCC CTGCGCGCAA GGCCGCCGTG GCGCCCGGGC CGTGGAAGGT GCCGGGCTCC
    GACAAGCTGC CGGGCGTCCT CAAACCCGGC GCCTCAGCCC CGGGGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318491.1
    CDS920..4930
    Translation

    Target ORF information:

    RefSeq Version XM_015463005.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463005.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGACAG CTCCTCATCG ACAGTACCTA GGCCCAAACC TGCCCGCTCC
    CTCGCCGCCC GGGAGCGGTC CCGCAGCGTG AGTGACCTGT CTTACTACGG GAGCCACCTC
    CCCGGGAGGC TCTGGCGCAT CGTAGAAAAG CCACTCTTTA CCCGGGATGC CTCCCAATTG
    AAGGGAACGT TTCTCAGCAC CACACTCAAA AAGAGCAACA TGGGGTTTGG ATTTACCATT
    ATTGGCGGAG ATGAGCCTGA CGAGTTTCTG CAGGTGAAAA GTGTGATTCC CGACGGGCCT
    GCAGCACAAG ACGGGAAAAT GGAAACAGGT GATGTCATTG TCTATATTAA TGAAGTTTGT
    GTCCTTGGAC ACACTCATGC AGATGTAGTC AAACTTTTCC AGTCTGTTCC TATTGGTCAG
    AGTGTCAACT TGGTGTTGTG TCGTGGCTAC CCTTTGCCCT TTGATCCTGA AGATCCTGCT
    AACAGCATGG TGCCACCCCT TGCAATAATG GAGAGGCCAC CTCCAGTGAT GGTCAATGGA
    AGACATAACT ATGAAACATA TTTGGAATAC ATTTCTCGGA CCTCACAGTC AGTTCCGGAT
    ATAACAGATC GGCCGCCTCA TACTTTGCAC TCAATGCCAG CTGATGGTCA GCTAGATGGC
    ACGTATCCAC CACCTGTCCA TGACGACAAT GTATCTATGG CTTCATCTGG GGCCACCCAA
    GCTGAACTTA TGACCTTAAC CATTGTGAAA GGTGCCCAGG GTTTTGGCTT CACCATTGCA
    GACAGTCCTA CAGGACAGCG GGTGAAACAA ATACTTGACA TTCAGGGATG CCCTGGCCTG
    TGTGAAGGCG ACCTCATTAT TGAGATCAAC CAGCAGAATG TACAGAACCT GAGCCATACG
    GAAGTAGTGG ATATACTTAA GGACTGTCCC ATTGGAAGCG AGACTTCTTT GATTATTCAT
    CGAGGAGGTT TCTTTTCTCC ATGGAAAACC CCAAAGCCTA TAATGGATCG ATGGGAGAAC
    CAAGGCAGTC CTCAAACGAG TTTATCTGCT CCGGCCATAC CACAGAACCT GCCCTTCCCA
    CCCGCCCTTC ACAGGAGTTC CTTTCCTGAC TCAACAGAGG CCTTTGACCC AAGGAAGCCT
    GACCCATATG AGCTCTACGA GAAATCGAGA GCCATTTATG AAAGTAGGCA ACAAGTGCCA
    CCAAGGACCA GTTTTCGAAT GGATTCTTCT GGTCCAGATT ATAAGGAGCT GGATGTTCAC
    CTTCGCAGGA TGGAGTCTGG ATTTGGCTTC AGAATCCTCG GGGGAGATGA GCCTGGACAG
    CCTATTTTGA TTGGAGCCGT CATCGCCATG GGCTCAGCTG ACAGAGATGG CCGCCTACAC
    CCAGGAGATG AGCTCGTCTA TGTGGATGGA ATTCCAGTGG CTGGAAAGAC CCACCGCTAT
    GTCATTGACC TTATGCACCA TGCAGCCCGG AATGGGCAGG TCAACCTCAC TGTGAGAAGA
    AAGGTGCTAT GTGGAGGGGA GCCCTGCCCA GAGAATGGGA GGAGTCCAGG CTCCGTGTCG
    ACCCACCACA GTTCTCCTCG CAGTGACTAC GCAACCTACA CCAACACCTA CAGCAACAGT
    AACCATGCCG TCCCCACCAG CAACGCCTCT CCCCCCGAAG GCTTCGCCTC CCACAGCCTG
    CAGACCAGCG ACGTGGTCAT TCACCGCAAA GAGAACGAGG GTTTCGGCTT TGTCATCATC
    AGCTCCCTGA ACAGGCCAGA GTCTGGATCC ACTATAACTG TGCCACATAA AATTGGACGC
    ATCATTGATG GGAGTCCCGC AGATCGCTGT GCAAAGCTAA AAGTGGGAGA CCGGATCTTA
    GCGGTGAACG GCCAGTCTAT CATCAACATG CCTCATGCAG ACATCGTGAA GCTCATCAAG
    GATGCGGGTC TTAGCGTCAC CCTTCGCATC ATCCCTCAGG AGGAACTCAA CAGCCCCGCT
    TCAGCACCCA GCTCAGAGAA GCAGAGCCCC CTGGCCCAGC AGCACAGCCC CCTGGCCCAA
    CAGAGTCCTC TGGCCCAGCC CAGCCCGGCC ACGCCCAACA GTCCCATCGC CCAGCCAGCT
    CCGCCTCAGC CTCTTCAGCC TCAAGGACAT GAAAATAGTT ACAGGTCAGA AGTAAAAGCG
    AGGCAAGATG TGAAACCAGA CATCCGACAG CCTCCATTCA CGGACTACAG GCAGCCTCCA
    CTGGACTACA GGCAACCCCC AGGAGGGGAC TACCCGCAGC CCCCACCCTT GGACTACAGG
    CAGCCTCCGC TACTGGACTA CAGGCAGCAC TCCCCAGACA CCAGGCAGTT CCCTCTGTCA
    GACTACAGGC AGCCCCAGGA TTTTGATTAT TTCACTGTGG ACATGGAGAA AGGAGCCAAA
    GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAGTACAAAA TGGATTTGTA TGTGCTGAGA
    TTAGCAGAAG ATGGGCCAGC AATAAGGAAT GGAAGGATGA GGGTAGGAGA TCAGATCATT
    GAAATCAATG GGGAGAGCAC AAGAGACATG ACCCATGCCA GAGCAATAGA GCTCATCAAA
    TCAGGCGGAA GGAGAGTGAG GCTACTGCTG AAGCGTGGGA CGGGACAGGT CCCAGAGTAT
    GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC CAGGTCTGCC GGAAGTAGGC
    GTCTCCCTGG AGGACGTCCT CTCTCCATTC TCTCCATCAC ATCCAGCCCC ACCCTCCGAC
    CCTTCCCACC AGACAAACCC AGACCCAACT TGGGATATCA AAAGGGAACA CGACGTTAGG
    AAACCAAAGG AGCTTTCAGC CGGCAGCCAG AAGAAGCAGC GCCTTGGGGA GCAGAGCGAG
    CGCTCAGCCA GCCCGCAAAG GGCCGTGCAA CCAGGGCTCG AGGAGGCGCC TGGCGGCCAG
    GGGCAGCCTG AGGCCGGCAG GCCCTCCTCG GAGACCAGAG CGCCTGGGCC CGCACTGGCC
    GAAGCGGCGC GGGCTGGCAA CAAGGAGGGG CTGCAGGCCC CGGTGGGCTC CGAGCTCGGC
    CGGCACGGAG GCCCCGGCGC GGCACCTGCG TTCGTGGGCC CGGGCCGCGG CGGGGGCGCA
    CGGGAGGTCG AGGGCCGGGC CGGGGCGCGG GCGGGGTCCA GGCAGGGCTC GCGGCCCCCC
    GGGGGCGCCC CTGCGCGCAA GGCCGCCGTG GCGCCCGGGC CGTGGAAGGT GCCGGGCTCC
    GACAAGCTGC CGGGCGTCCT CAAACCCGGC GCCTCAGCCC CGGGGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89707
    Clone ID Related Accession (Same CDS sequence) XM_015463006.1 , XM_015468990.1
    Accession Version XM_015463006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAAACA AGTGCCACCA
    AGGACCAGTT TTCGAATGGA TTCTTCTGGT CCAGATTATA AGGAGCTGGA TGTTCACCTT
    CGCAGGATGG AGTCTGGATT TGGCTTCAGA ATCCTCGGGG GAGATGAGCC TGGACAGCCT
    ATTTTGATTG GAGCCGTCAT CGCCATGGGC TCAGCTGACA GAGATGGCCG CCTACACCCA
    GGAGATGAGC TCGTCTATGT GGATGGAATT CCAGTGGCTG GAAAGACCCA CCGCTATGTC
    ATTGACCTTA TGCACCATGC AGCCCGGAAT GGGCAGGTCA ACCTCACTGT GAGAAGAAAG
    GTGCTATGTG GAGGGGAGCC CTGCCCAGAG AATGGGAGGA GTCCAGGCTC CGTGTCGACC
    CACCACAGTT CTCCTCGCAG TGACTACGCA ACCTACACCA ACACCTACAG CAACAGTAAC
    CATGCCGTCC CCACCAGCAA CGCCTCTCCC CCCGAAGGCT TCGCCTCCCA CAGCCTGCAG
    ACCAGCGACG TGGTCATTCA CCGCAAAGAG AACGAGGGTT TCGGCTTTGT CATCATCAGC
    TCCCTGAACA GGCCAGAGTC TGGATCCACT ATAACTGTGC CACATAAAAT TGGACGCATC
    ATTGATGGGA GTCCCGCAGA TCGCTGTGCA AAGCTAAAAG TGGGAGACCG GATCTTAGCG
    GTGAACGGCC AGTCTATCAT CAACATGCCT CATGCAGACA TCGTGAAGCT CATCAAGGAT
    GCGGGTCTTA GCGTCACCCT TCGCATCATC CCTCAGGAGG AACTCAACAG CCCCGCTTCA
    GCACCCAGCT CAGAGAAGCA GAGCCCCCTG GCCCAGCAGC ACAGCCCCCT GGCCCAACAG
    AGTCCTCTGG CCCAGCCCAG CCCGGCCACG CCCAACAGTC CCATCGCCCA GCCAGCTCCG
    CCTCAGCCTC TTCAGCCTCA AGGACATGAA AATAGTTACA GGTCAGAAGT AAAAGCGAGG
    CAAGATGTGA AACCAGACAT CCGACAGCCT CCATTCACGG ACTACAGGCA GCCTCCACTG
    GACTACAGGC AACCCCCAGG AGGGGACTAC CCGCAGCCCC CACCCTTGGA CTACAGGCAG
    CCTCCGCTAC TGGACTACAG GCAGCACTCC CCAGACACCA GGCAGTTCCC TCTGTCAGAC
    TACAGGCAGC CCCAGGATTT TGATTATTTC ACTGTGGACA TGGAGAAAGG AGCCAAAGGA
    TTTGGATTCA GCATTCGTGG AGGAAGGGAG TACAAAATGG ATTTGTATGT GCTGAGATTA
    GCAGAAGATG GGCCAGCAAT AAGGAATGGA AGGATGAGGG TAGGAGATCA GATCATTGAA
    ATCAATGGGG AGAGCACAAG AGACATGACC CATGCCAGAG CAATAGAGCT CATCAAATCA
    GGCGGAAGGA GAGTGAGGCT ACTGCTGAAG CGTGGGACGG GACAGGTCCC AGAGTATGAC
    GAACCCGGCT CCTGGAGTGC TCCCGCTGCC GCCTCCCCAG GTCTGCCGGA AGTAGGCGTC
    TCCCTGGAGG ACGTCCTCTC TCCATTCTCT CCATCACATC CAGCCCCACC CTCCGACCCT
    TCCCACCAGA CAAACCCAGA CCCAACTTGG GATATCAAAA GGGAACACGA CGTTAGGAAA
    CCAAAGGAGC TTTCAGCCGG CAGCCAGAAG AAGCAGCGCC TTGGGGAGCA GAGCGAGCGC
    TCAGCCAGCC CGCAAAGGGC CGTGCAACCA GGGCTCGAGG AGGCGCCTGG CGGCCAGGGG
    CAGCCTGAGG CCGGCAGGCC CTCCTCGGAG ACCAGAGCGC CTGGGCCCGC ACTGGCCGAA
    GCGGCGCGGG CTGGCAACAA GGAGGGGCTG CAGGCCCCGG TGGGCTCCGA GCTCGGCCGG
    CACGGAGGCC CCGGCGCGGC ACCTGCGTTC GTGGGCCCGG GCCGCGGCGG GGGCGCACGG
    GAGGTCGAGG GCCGGGCCGG GGCGCGGGCG GGGTCCAGGC AGGGCTCGCG GCCCCCCGGG
    GGCGCCCCTG CGCGCAAGGC CGCCGTGGCG CCCGGGCCGT GGAAGGTGCC GGGCTCCGAC
    AAGCTGCCGG GCGTCCTCAA ACCCGGCGCC TCAGCCCCGG GGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318492.1
    CDS920..4927
    Translation

    Target ORF information:

    RefSeq Version XM_015463006.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAAACA AGTGCCACCA
    AGGACCAGTT TTCGAATGGA TTCTTCTGGT CCAGATTATA AGGAGCTGGA TGTTCACCTT
    CGCAGGATGG AGTCTGGATT TGGCTTCAGA ATCCTCGGGG GAGATGAGCC TGGACAGCCT
    ATTTTGATTG GAGCCGTCAT CGCCATGGGC TCAGCTGACA GAGATGGCCG CCTACACCCA
    GGAGATGAGC TCGTCTATGT GGATGGAATT CCAGTGGCTG GAAAGACCCA CCGCTATGTC
    ATTGACCTTA TGCACCATGC AGCCCGGAAT GGGCAGGTCA ACCTCACTGT GAGAAGAAAG
    GTGCTATGTG GAGGGGAGCC CTGCCCAGAG AATGGGAGGA GTCCAGGCTC CGTGTCGACC
    CACCACAGTT CTCCTCGCAG TGACTACGCA ACCTACACCA ACACCTACAG CAACAGTAAC
    CATGCCGTCC CCACCAGCAA CGCCTCTCCC CCCGAAGGCT TCGCCTCCCA CAGCCTGCAG
    ACCAGCGACG TGGTCATTCA CCGCAAAGAG AACGAGGGTT TCGGCTTTGT CATCATCAGC
    TCCCTGAACA GGCCAGAGTC TGGATCCACT ATAACTGTGC CACATAAAAT TGGACGCATC
    ATTGATGGGA GTCCCGCAGA TCGCTGTGCA AAGCTAAAAG TGGGAGACCG GATCTTAGCG
    GTGAACGGCC AGTCTATCAT CAACATGCCT CATGCAGACA TCGTGAAGCT CATCAAGGAT
    GCGGGTCTTA GCGTCACCCT TCGCATCATC CCTCAGGAGG AACTCAACAG CCCCGCTTCA
    GCACCCAGCT CAGAGAAGCA GAGCCCCCTG GCCCAGCAGC ACAGCCCCCT GGCCCAACAG
    AGTCCTCTGG CCCAGCCCAG CCCGGCCACG CCCAACAGTC CCATCGCCCA GCCAGCTCCG
    CCTCAGCCTC TTCAGCCTCA AGGACATGAA AATAGTTACA GGTCAGAAGT AAAAGCGAGG
    CAAGATGTGA AACCAGACAT CCGACAGCCT CCATTCACGG ACTACAGGCA GCCTCCACTG
    GACTACAGGC AACCCCCAGG AGGGGACTAC CCGCAGCCCC CACCCTTGGA CTACAGGCAG
    CCTCCGCTAC TGGACTACAG GCAGCACTCC CCAGACACCA GGCAGTTCCC TCTGTCAGAC
    TACAGGCAGC CCCAGGATTT TGATTATTTC ACTGTGGACA TGGAGAAAGG AGCCAAAGGA
    TTTGGATTCA GCATTCGTGG AGGAAGGGAG TACAAAATGG ATTTGTATGT GCTGAGATTA
    GCAGAAGATG GGCCAGCAAT AAGGAATGGA AGGATGAGGG TAGGAGATCA GATCATTGAA
    ATCAATGGGG AGAGCACAAG AGACATGACC CATGCCAGAG CAATAGAGCT CATCAAATCA
    GGCGGAAGGA GAGTGAGGCT ACTGCTGAAG CGTGGGACGG GACAGGTCCC AGAGTATGAC
    GAACCCGGCT CCTGGAGTGC TCCCGCTGCC GCCTCCCCAG GTCTGCCGGA AGTAGGCGTC
    TCCCTGGAGG ACGTCCTCTC TCCATTCTCT CCATCACATC CAGCCCCACC CTCCGACCCT
    TCCCACCAGA CAAACCCAGA CCCAACTTGG GATATCAAAA GGGAACACGA CGTTAGGAAA
    CCAAAGGAGC TTTCAGCCGG CAGCCAGAAG AAGCAGCGCC TTGGGGAGCA GAGCGAGCGC
    TCAGCCAGCC CGCAAAGGGC CGTGCAACCA GGGCTCGAGG AGGCGCCTGG CGGCCAGGGG
    CAGCCTGAGG CCGGCAGGCC CTCCTCGGAG ACCAGAGCGC CTGGGCCCGC ACTGGCCGAA
    GCGGCGCGGG CTGGCAACAA GGAGGGGCTG CAGGCCCCGG TGGGCTCCGA GCTCGGCCGG
    CACGGAGGCC CCGGCGCGGC ACCTGCGTTC GTGGGCCCGG GCCGCGGCGG GGGCGCACGG
    GAGGTCGAGG GCCGGGCCGG GGCGCGGGCG GGGTCCAGGC AGGGCTCGCG GCCCCCCGGG
    GGCGCCCCTG CGCGCAAGGC CGCCGTGGCG CCCGGGCCGT GGAAGGTGCC GGGCTCCGAC
    AAGCTGCCGG GCGTCCTCAA ACCCGGCGCC TCAGCCCCGG GGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89707
    Clone ID Related Accession (Same CDS sequence) XM_015463006.1 , XM_015468990.1
    Accession Version XM_015468990.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAAACA AGTGCCACCA
    AGGACCAGTT TTCGAATGGA TTCTTCTGGT CCAGATTATA AGGAGCTGGA TGTTCACCTT
    CGCAGGATGG AGTCTGGATT TGGCTTCAGA ATCCTCGGGG GAGATGAGCC TGGACAGCCT
    ATTTTGATTG GAGCCGTCAT CGCCATGGGC TCAGCTGACA GAGATGGCCG CCTACACCCA
    GGAGATGAGC TCGTCTATGT GGATGGAATT CCAGTGGCTG GAAAGACCCA CCGCTATGTC
    ATTGACCTTA TGCACCATGC AGCCCGGAAT GGGCAGGTCA ACCTCACTGT GAGAAGAAAG
    GTGCTATGTG GAGGGGAGCC CTGCCCAGAG AATGGGAGGA GTCCAGGCTC CGTGTCGACC
    CACCACAGTT CTCCTCGCAG TGACTACGCA ACCTACACCA ACACCTACAG CAACAGTAAC
    CATGCCGTCC CCACCAGCAA CGCCTCTCCC CCCGAAGGCT TCGCCTCCCA CAGCCTGCAG
    ACCAGCGACG TGGTCATTCA CCGCAAAGAG AACGAGGGTT TCGGCTTTGT CATCATCAGC
    TCCCTGAACA GGCCAGAGTC TGGATCCACT ATAACTGTGC CACATAAAAT TGGACGCATC
    ATTGATGGGA GTCCCGCAGA TCGCTGTGCA AAGCTAAAAG TGGGAGACCG GATCTTAGCG
    GTGAACGGCC AGTCTATCAT CAACATGCCT CATGCAGACA TCGTGAAGCT CATCAAGGAT
    GCGGGTCTTA GCGTCACCCT TCGCATCATC CCTCAGGAGG AACTCAACAG CCCCGCTTCA
    GCACCCAGCT CAGAGAAGCA GAGCCCCCTG GCCCAGCAGC ACAGCCCCCT GGCCCAACAG
    AGTCCTCTGG CCCAGCCCAG CCCGGCCACG CCCAACAGTC CCATCGCCCA GCCAGCTCCG
    CCTCAGCCTC TTCAGCCTCA AGGACATGAA AATAGTTACA GGTCAGAAGT AAAAGCGAGG
    CAAGATGTGA AACCAGACAT CCGACAGCCT CCATTCACGG ACTACAGGCA GCCTCCACTG
    GACTACAGGC AACCCCCAGG AGGGGACTAC CCGCAGCCCC CACCCTTGGA CTACAGGCAG
    CCTCCGCTAC TGGACTACAG GCAGCACTCC CCAGACACCA GGCAGTTCCC TCTGTCAGAC
    TACAGGCAGC CCCAGGATTT TGATTATTTC ACTGTGGACA TGGAGAAAGG AGCCAAAGGA
    TTTGGATTCA GCATTCGTGG AGGAAGGGAG TACAAAATGG ATTTGTATGT GCTGAGATTA
    GCAGAAGATG GGCCAGCAAT AAGGAATGGA AGGATGAGGG TAGGAGATCA GATCATTGAA
    ATCAATGGGG AGAGCACAAG AGACATGACC CATGCCAGAG CAATAGAGCT CATCAAATCA
    GGCGGAAGGA GAGTGAGGCT ACTGCTGAAG CGTGGGACGG GACAGGTCCC AGAGTATGAC
    GAACCCGGCT CCTGGAGTGC TCCCGCTGCC GCCTCCCCAG GTCTGCCGGA AGTAGGCGTC
    TCCCTGGAGG ACGTCCTCTC TCCATTCTCT CCATCACATC CAGCCCCACC CTCCGACCCT
    TCCCACCAGA CAAACCCAGA CCCAACTTGG GATATCAAAA GGGAACACGA CGTTAGGAAA
    CCAAAGGAGC TTTCAGCCGG CAGCCAGAAG AAGCAGCGCC TTGGGGAGCA GAGCGAGCGC
    TCAGCCAGCC CGCAAAGGGC CGTGCAACCA GGGCTCGAGG AGGCGCCTGG CGGCCAGGGG
    CAGCCTGAGG CCGGCAGGCC CTCCTCGGAG ACCAGAGCGC CTGGGCCCGC ACTGGCCGAA
    GCGGCGCGGG CTGGCAACAA GGAGGGGCTG CAGGCCCCGG TGGGCTCCGA GCTCGGCCGG
    CACGGAGGCC CCGGCGCGGC ACCTGCGTTC GTGGGCCCGG GCCGCGGCGG GGGCGCACGG
    GAGGTCGAGG GCCGGGCCGG GGCGCGGGCG GGGTCCAGGC AGGGCTCGCG GCCCCCCGGG
    GGCGCCCCTG CGCGCAAGGC CGCCGTGGCG CCCGGGCCGT GGAAGGTGCC GGGCTCCGAC
    AAGCTGCCGG GCGTCCTCAA ACCCGGCGCC TCAGCCCCGG GGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324476.1
    CDS920..4927
    Translation

    Target ORF information:

    RefSeq Version XM_015468990.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAAACA AGTGCCACCA
    AGGACCAGTT TTCGAATGGA TTCTTCTGGT CCAGATTATA AGGAGCTGGA TGTTCACCTT
    CGCAGGATGG AGTCTGGATT TGGCTTCAGA ATCCTCGGGG GAGATGAGCC TGGACAGCCT
    ATTTTGATTG GAGCCGTCAT CGCCATGGGC TCAGCTGACA GAGATGGCCG CCTACACCCA
    GGAGATGAGC TCGTCTATGT GGATGGAATT CCAGTGGCTG GAAAGACCCA CCGCTATGTC
    ATTGACCTTA TGCACCATGC AGCCCGGAAT GGGCAGGTCA ACCTCACTGT GAGAAGAAAG
    GTGCTATGTG GAGGGGAGCC CTGCCCAGAG AATGGGAGGA GTCCAGGCTC CGTGTCGACC
    CACCACAGTT CTCCTCGCAG TGACTACGCA ACCTACACCA ACACCTACAG CAACAGTAAC
    CATGCCGTCC CCACCAGCAA CGCCTCTCCC CCCGAAGGCT TCGCCTCCCA CAGCCTGCAG
    ACCAGCGACG TGGTCATTCA CCGCAAAGAG AACGAGGGTT TCGGCTTTGT CATCATCAGC
    TCCCTGAACA GGCCAGAGTC TGGATCCACT ATAACTGTGC CACATAAAAT TGGACGCATC
    ATTGATGGGA GTCCCGCAGA TCGCTGTGCA AAGCTAAAAG TGGGAGACCG GATCTTAGCG
    GTGAACGGCC AGTCTATCAT CAACATGCCT CATGCAGACA TCGTGAAGCT CATCAAGGAT
    GCGGGTCTTA GCGTCACCCT TCGCATCATC CCTCAGGAGG AACTCAACAG CCCCGCTTCA
    GCACCCAGCT CAGAGAAGCA GAGCCCCCTG GCCCAGCAGC ACAGCCCCCT GGCCCAACAG
    AGTCCTCTGG CCCAGCCCAG CCCGGCCACG CCCAACAGTC CCATCGCCCA GCCAGCTCCG
    CCTCAGCCTC TTCAGCCTCA AGGACATGAA AATAGTTACA GGTCAGAAGT AAAAGCGAGG
    CAAGATGTGA AACCAGACAT CCGACAGCCT CCATTCACGG ACTACAGGCA GCCTCCACTG
    GACTACAGGC AACCCCCAGG AGGGGACTAC CCGCAGCCCC CACCCTTGGA CTACAGGCAG
    CCTCCGCTAC TGGACTACAG GCAGCACTCC CCAGACACCA GGCAGTTCCC TCTGTCAGAC
    TACAGGCAGC CCCAGGATTT TGATTATTTC ACTGTGGACA TGGAGAAAGG AGCCAAAGGA
    TTTGGATTCA GCATTCGTGG AGGAAGGGAG TACAAAATGG ATTTGTATGT GCTGAGATTA
    GCAGAAGATG GGCCAGCAAT AAGGAATGGA AGGATGAGGG TAGGAGATCA GATCATTGAA
    ATCAATGGGG AGAGCACAAG AGACATGACC CATGCCAGAG CAATAGAGCT CATCAAATCA
    GGCGGAAGGA GAGTGAGGCT ACTGCTGAAG CGTGGGACGG GACAGGTCCC AGAGTATGAC
    GAACCCGGCT CCTGGAGTGC TCCCGCTGCC GCCTCCCCAG GTCTGCCGGA AGTAGGCGTC
    TCCCTGGAGG ACGTCCTCTC TCCATTCTCT CCATCACATC CAGCCCCACC CTCCGACCCT
    TCCCACCAGA CAAACCCAGA CCCAACTTGG GATATCAAAA GGGAACACGA CGTTAGGAAA
    CCAAAGGAGC TTTCAGCCGG CAGCCAGAAG AAGCAGCGCC TTGGGGAGCA GAGCGAGCGC
    TCAGCCAGCC CGCAAAGGGC CGTGCAACCA GGGCTCGAGG AGGCGCCTGG CGGCCAGGGG
    CAGCCTGAGG CCGGCAGGCC CTCCTCGGAG ACCAGAGCGC CTGGGCCCGC ACTGGCCGAA
    GCGGCGCGGG CTGGCAACAA GGAGGGGCTG CAGGCCCCGG TGGGCTCCGA GCTCGGCCGG
    CACGGAGGCC CCGGCGCGGC ACCTGCGTTC GTGGGCCCGG GCCGCGGCGG GGGCGCACGG
    GAGGTCGAGG GCCGGGCCGG GGCGCGGGCG GGGTCCAGGC AGGGCTCGCG GCCCCCCGGG
    GGCGCCCCTG CGCGCAAGGC CGCCGTGGCG CCCGGGCCGT GGAAGGTGCC GGGCTCCGAC
    AAGCTGCCGG GCGTCCTCAA ACCCGGCGCC TCAGCCCCGG GGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89708
    Clone ID Related Accession (Same CDS sequence) XM_015463007.1 , XM_015468991.1
    Accession Version XM_015463007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCGTCCAGAT
    TATAAGGAGC TGGATGTTCA CCTTCGCAGG ATGGAGTCTG GATTTGGCTT CAGAATCCTC
    GGGGGAGATG AGCCTGGACA GCCTATTTTG ATTGGAGCCG TCATCGCCAT GGGCTCAGCT
    GACAGAGATG GCCGCCTACA CCCAGGAGAT GAGCTCGTCT ATGTGGATGG AATTCCAGTG
    GCTGGAAAGA CCCACCGCTA TGTCATTGAC CTTATGCACC ATGCAGCCCG GAATGGGCAG
    GTCAACCTCA CTGTGAGAAG AAAGGTGCTA TGTGGAGGGG AGCCCTGCCC AGAGAATGGG
    AGGAGTCCAG GCTCCGTGTC GACCCACCAC AGTTCTCCTC GCAGTGACTA CGCAACCTAC
    ACCAACACCT ACAGCAACAG TAACCATGCC GTCCCCACCA GCAACGCCTC TCCCCCCGAA
    GGCTTCGCCT CCCACAGCCT GCAGACCAGC GACGTGGTCA TTCACCGCAA AGAGAACGAG
    GGTTTCGGCT TTGTCATCAT CAGCTCCCTG AACAGGCCAG AGTCTGGATC CACTATAACT
    GTGCCACATA AAATTGGACG CATCATTGAT GGGAGTCCCG CAGATCGCTG TGCAAAGCTA
    AAAGTGGGAG ACCGGATCTT AGCGGTGAAC GGCCAGTCTA TCATCAACAT GCCTCATGCA
    GACATCGTGA AGCTCATCAA GGATGCGGGT CTTAGCGTCA CCCTTCGCAT CATCCCTCAG
    GAGGAACTCA ACAGCCCCGC TTCAGCACCC AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG
    CAGCACAGCC CCCTGGCCCA ACAGAGTCCT CTGGCCCAGC CCAGCCCGGC CACGCCCAAC
    AGTCCCATCG CCCAGCCAGC TCCGCCTCAG CCTCTTCAGC CTCAAGGACA TGAAAATAGT
    TACAGGTCAG AAGTAAAAGC GAGGCAAGAT GTGAAACCAG ACATCCGACA GCCTCCATTC
    ACGGACTACA GGCAGCCTCC ACTGGACTAC AGGCAACCCC CAGGAGGGGA CTACCCGCAG
    CCCCCACCCT TGGACTACAG GCAGCCTCCG CTACTGGACT ACAGGCAGCA CTCCCCAGAC
    ACCAGGCAGT TCCCTCTGTC AGACTACAGG CAGCCCCAGG ATTTTGATTA TTTCACTGTG
    GACATGGAGA AAGGAGCCAA AGGATTTGGA TTCAGCATTC GTGGAGGAAG GGAGTACAAA
    ATGGATTTGT ATGTGCTGAG ATTAGCAGAA GATGGGCCAG CAATAAGGAA TGGAAGGATG
    AGGGTAGGAG ATCAGATCAT TGAAATCAAT GGGGAGAGCA CAAGAGACAT GACCCATGCC
    AGAGCAATAG AGCTCATCAA ATCAGGCGGA AGGAGAGTGA GGCTACTGCT GAAGCGTGGG
    ACGGGACAGG TCCCAGAGTA TGACGAACCC GGCTCCTGGA GTGCTCCCGC TGCCGCCTCC
    CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG GAGGACGTCC TCTCTCCATT CTCTCCATCA
    CATCCAGCCC CACCCTCCGA CCCTTCCCAC CAGACAAACC CAGACCCAAC TTGGGATATC
    AAAAGGGAAC ACGACGTTAG GAAACCAAAG GAGCTTTCAG CCGGCAGCCA GAAGAAGCAG
    CGCCTTGGGG AGCAGAGCGA GCGCTCAGCC AGCCCGCAAA GGGCCGTGCA ACCAGGGCTC
    GAGGAGGCGC CTGGCGGCCA GGGGCAGCCT GAGGCCGGCA GGCCCTCCTC GGAGACCAGA
    GCGCCTGGGC CCGCACTGGC CGAAGCGGCG CGGGCTGGCA ACAAGGAGGG GCTGCAGGCC
    CCGGTGGGCT CCGAGCTCGG CCGGCACGGA GGCCCCGGCG CGGCACCTGC GTTCGTGGGC
    CCGGGCCGCG GCGGGGGCGC ACGGGAGGTC GAGGGCCGGG CCGGGGCGCG GGCGGGGTCC
    AGGCAGGGCT CGCGGCCCCC CGGGGGCGCC CCTGCGCGCA AGGCCGCCGT GGCGCCCGGG
    CCGTGGAAGG TGCCGGGCTC CGACAAGCTG CCGGGCGTCC TCAAACCCGG CGCCTCAGCC
    CCGGGGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318493.1
    CDS918..4889
    Translation

    Target ORF information:

    RefSeq Version XM_015463007.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCGTCCAGAT
    TATAAGGAGC TGGATGTTCA CCTTCGCAGG ATGGAGTCTG GATTTGGCTT CAGAATCCTC
    GGGGGAGATG AGCCTGGACA GCCTATTTTG ATTGGAGCCG TCATCGCCAT GGGCTCAGCT
    GACAGAGATG GCCGCCTACA CCCAGGAGAT GAGCTCGTCT ATGTGGATGG AATTCCAGTG
    GCTGGAAAGA CCCACCGCTA TGTCATTGAC CTTATGCACC ATGCAGCCCG GAATGGGCAG
    GTCAACCTCA CTGTGAGAAG AAAGGTGCTA TGTGGAGGGG AGCCCTGCCC AGAGAATGGG
    AGGAGTCCAG GCTCCGTGTC GACCCACCAC AGTTCTCCTC GCAGTGACTA CGCAACCTAC
    ACCAACACCT ACAGCAACAG TAACCATGCC GTCCCCACCA GCAACGCCTC TCCCCCCGAA
    GGCTTCGCCT CCCACAGCCT GCAGACCAGC GACGTGGTCA TTCACCGCAA AGAGAACGAG
    GGTTTCGGCT TTGTCATCAT CAGCTCCCTG AACAGGCCAG AGTCTGGATC CACTATAACT
    GTGCCACATA AAATTGGACG CATCATTGAT GGGAGTCCCG CAGATCGCTG TGCAAAGCTA
    AAAGTGGGAG ACCGGATCTT AGCGGTGAAC GGCCAGTCTA TCATCAACAT GCCTCATGCA
    GACATCGTGA AGCTCATCAA GGATGCGGGT CTTAGCGTCA CCCTTCGCAT CATCCCTCAG
    GAGGAACTCA ACAGCCCCGC TTCAGCACCC AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG
    CAGCACAGCC CCCTGGCCCA ACAGAGTCCT CTGGCCCAGC CCAGCCCGGC CACGCCCAAC
    AGTCCCATCG CCCAGCCAGC TCCGCCTCAG CCTCTTCAGC CTCAAGGACA TGAAAATAGT
    TACAGGTCAG AAGTAAAAGC GAGGCAAGAT GTGAAACCAG ACATCCGACA GCCTCCATTC
    ACGGACTACA GGCAGCCTCC ACTGGACTAC AGGCAACCCC CAGGAGGGGA CTACCCGCAG
    CCCCCACCCT TGGACTACAG GCAGCCTCCG CTACTGGACT ACAGGCAGCA CTCCCCAGAC
    ACCAGGCAGT TCCCTCTGTC AGACTACAGG CAGCCCCAGG ATTTTGATTA TTTCACTGTG
    GACATGGAGA AAGGAGCCAA AGGATTTGGA TTCAGCATTC GTGGAGGAAG GGAGTACAAA
    ATGGATTTGT ATGTGCTGAG ATTAGCAGAA GATGGGCCAG CAATAAGGAA TGGAAGGATG
    AGGGTAGGAG ATCAGATCAT TGAAATCAAT GGGGAGAGCA CAAGAGACAT GACCCATGCC
    AGAGCAATAG AGCTCATCAA ATCAGGCGGA AGGAGAGTGA GGCTACTGCT GAAGCGTGGG
    ACGGGACAGG TCCCAGAGTA TGACGAACCC GGCTCCTGGA GTGCTCCCGC TGCCGCCTCC
    CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG GAGGACGTCC TCTCTCCATT CTCTCCATCA
    CATCCAGCCC CACCCTCCGA CCCTTCCCAC CAGACAAACC CAGACCCAAC TTGGGATATC
    AAAAGGGAAC ACGACGTTAG GAAACCAAAG GAGCTTTCAG CCGGCAGCCA GAAGAAGCAG
    CGCCTTGGGG AGCAGAGCGA GCGCTCAGCC AGCCCGCAAA GGGCCGTGCA ACCAGGGCTC
    GAGGAGGCGC CTGGCGGCCA GGGGCAGCCT GAGGCCGGCA GGCCCTCCTC GGAGACCAGA
    GCGCCTGGGC CCGCACTGGC CGAAGCGGCG CGGGCTGGCA ACAAGGAGGG GCTGCAGGCC
    CCGGTGGGCT CCGAGCTCGG CCGGCACGGA GGCCCCGGCG CGGCACCTGC GTTCGTGGGC
    CCGGGCCGCG GCGGGGGCGC ACGGGAGGTC GAGGGCCGGG CCGGGGCGCG GGCGGGGTCC
    AGGCAGGGCT CGCGGCCCCC CGGGGGCGCC CCTGCGCGCA AGGCCGCCGT GGCGCCCGGG
    CCGTGGAAGG TGCCGGGCTC CGACAAGCTG CCGGGCGTCC TCAAACCCGG CGCCTCAGCC
    CCGGGGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89708
    Clone ID Related Accession (Same CDS sequence) XM_015463007.1 , XM_015468991.1
    Accession Version XM_015468991.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCGTCCAGAT
    TATAAGGAGC TGGATGTTCA CCTTCGCAGG ATGGAGTCTG GATTTGGCTT CAGAATCCTC
    GGGGGAGATG AGCCTGGACA GCCTATTTTG ATTGGAGCCG TCATCGCCAT GGGCTCAGCT
    GACAGAGATG GCCGCCTACA CCCAGGAGAT GAGCTCGTCT ATGTGGATGG AATTCCAGTG
    GCTGGAAAGA CCCACCGCTA TGTCATTGAC CTTATGCACC ATGCAGCCCG GAATGGGCAG
    GTCAACCTCA CTGTGAGAAG AAAGGTGCTA TGTGGAGGGG AGCCCTGCCC AGAGAATGGG
    AGGAGTCCAG GCTCCGTGTC GACCCACCAC AGTTCTCCTC GCAGTGACTA CGCAACCTAC
    ACCAACACCT ACAGCAACAG TAACCATGCC GTCCCCACCA GCAACGCCTC TCCCCCCGAA
    GGCTTCGCCT CCCACAGCCT GCAGACCAGC GACGTGGTCA TTCACCGCAA AGAGAACGAG
    GGTTTCGGCT TTGTCATCAT CAGCTCCCTG AACAGGCCAG AGTCTGGATC CACTATAACT
    GTGCCACATA AAATTGGACG CATCATTGAT GGGAGTCCCG CAGATCGCTG TGCAAAGCTA
    AAAGTGGGAG ACCGGATCTT AGCGGTGAAC GGCCAGTCTA TCATCAACAT GCCTCATGCA
    GACATCGTGA AGCTCATCAA GGATGCGGGT CTTAGCGTCA CCCTTCGCAT CATCCCTCAG
    GAGGAACTCA ACAGCCCCGC TTCAGCACCC AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG
    CAGCACAGCC CCCTGGCCCA ACAGAGTCCT CTGGCCCAGC CCAGCCCGGC CACGCCCAAC
    AGTCCCATCG CCCAGCCAGC TCCGCCTCAG CCTCTTCAGC CTCAAGGACA TGAAAATAGT
    TACAGGTCAG AAGTAAAAGC GAGGCAAGAT GTGAAACCAG ACATCCGACA GCCTCCATTC
    ACGGACTACA GGCAGCCTCC ACTGGACTAC AGGCAACCCC CAGGAGGGGA CTACCCGCAG
    CCCCCACCCT TGGACTACAG GCAGCCTCCG CTACTGGACT ACAGGCAGCA CTCCCCAGAC
    ACCAGGCAGT TCCCTCTGTC AGACTACAGG CAGCCCCAGG ATTTTGATTA TTTCACTGTG
    GACATGGAGA AAGGAGCCAA AGGATTTGGA TTCAGCATTC GTGGAGGAAG GGAGTACAAA
    ATGGATTTGT ATGTGCTGAG ATTAGCAGAA GATGGGCCAG CAATAAGGAA TGGAAGGATG
    AGGGTAGGAG ATCAGATCAT TGAAATCAAT GGGGAGAGCA CAAGAGACAT GACCCATGCC
    AGAGCAATAG AGCTCATCAA ATCAGGCGGA AGGAGAGTGA GGCTACTGCT GAAGCGTGGG
    ACGGGACAGG TCCCAGAGTA TGACGAACCC GGCTCCTGGA GTGCTCCCGC TGCCGCCTCC
    CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG GAGGACGTCC TCTCTCCATT CTCTCCATCA
    CATCCAGCCC CACCCTCCGA CCCTTCCCAC CAGACAAACC CAGACCCAAC TTGGGATATC
    AAAAGGGAAC ACGACGTTAG GAAACCAAAG GAGCTTTCAG CCGGCAGCCA GAAGAAGCAG
    CGCCTTGGGG AGCAGAGCGA GCGCTCAGCC AGCCCGCAAA GGGCCGTGCA ACCAGGGCTC
    GAGGAGGCGC CTGGCGGCCA GGGGCAGCCT GAGGCCGGCA GGCCCTCCTC GGAGACCAGA
    GCGCCTGGGC CCGCACTGGC CGAAGCGGCG CGGGCTGGCA ACAAGGAGGG GCTGCAGGCC
    CCGGTGGGCT CCGAGCTCGG CCGGCACGGA GGCCCCGGCG CGGCACCTGC GTTCGTGGGC
    CCGGGCCGCG GCGGGGGCGC ACGGGAGGTC GAGGGCCGGG CCGGGGCGCG GGCGGGGTCC
    AGGCAGGGCT CGCGGCCCCC CGGGGGCGCC CCTGCGCGCA AGGCCGCCGT GGCGCCCGGG
    CCGTGGAAGG TGCCGGGCTC CGACAAGCTG CCGGGCGTCC TCAAACCCGG CGCCTCAGCC
    CCGGGGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324477.1
    CDS918..4889
    Translation

    Target ORF information:

    RefSeq Version XM_015468991.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCGTCCAGAT
    TATAAGGAGC TGGATGTTCA CCTTCGCAGG ATGGAGTCTG GATTTGGCTT CAGAATCCTC
    GGGGGAGATG AGCCTGGACA GCCTATTTTG ATTGGAGCCG TCATCGCCAT GGGCTCAGCT
    GACAGAGATG GCCGCCTACA CCCAGGAGAT GAGCTCGTCT ATGTGGATGG AATTCCAGTG
    GCTGGAAAGA CCCACCGCTA TGTCATTGAC CTTATGCACC ATGCAGCCCG GAATGGGCAG
    GTCAACCTCA CTGTGAGAAG AAAGGTGCTA TGTGGAGGGG AGCCCTGCCC AGAGAATGGG
    AGGAGTCCAG GCTCCGTGTC GACCCACCAC AGTTCTCCTC GCAGTGACTA CGCAACCTAC
    ACCAACACCT ACAGCAACAG TAACCATGCC GTCCCCACCA GCAACGCCTC TCCCCCCGAA
    GGCTTCGCCT CCCACAGCCT GCAGACCAGC GACGTGGTCA TTCACCGCAA AGAGAACGAG
    GGTTTCGGCT TTGTCATCAT CAGCTCCCTG AACAGGCCAG AGTCTGGATC CACTATAACT
    GTGCCACATA AAATTGGACG CATCATTGAT GGGAGTCCCG CAGATCGCTG TGCAAAGCTA
    AAAGTGGGAG ACCGGATCTT AGCGGTGAAC GGCCAGTCTA TCATCAACAT GCCTCATGCA
    GACATCGTGA AGCTCATCAA GGATGCGGGT CTTAGCGTCA CCCTTCGCAT CATCCCTCAG
    GAGGAACTCA ACAGCCCCGC TTCAGCACCC AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG
    CAGCACAGCC CCCTGGCCCA ACAGAGTCCT CTGGCCCAGC CCAGCCCGGC CACGCCCAAC
    AGTCCCATCG CCCAGCCAGC TCCGCCTCAG CCTCTTCAGC CTCAAGGACA TGAAAATAGT
    TACAGGTCAG AAGTAAAAGC GAGGCAAGAT GTGAAACCAG ACATCCGACA GCCTCCATTC
    ACGGACTACA GGCAGCCTCC ACTGGACTAC AGGCAACCCC CAGGAGGGGA CTACCCGCAG
    CCCCCACCCT TGGACTACAG GCAGCCTCCG CTACTGGACT ACAGGCAGCA CTCCCCAGAC
    ACCAGGCAGT TCCCTCTGTC AGACTACAGG CAGCCCCAGG ATTTTGATTA TTTCACTGTG
    GACATGGAGA AAGGAGCCAA AGGATTTGGA TTCAGCATTC GTGGAGGAAG GGAGTACAAA
    ATGGATTTGT ATGTGCTGAG ATTAGCAGAA GATGGGCCAG CAATAAGGAA TGGAAGGATG
    AGGGTAGGAG ATCAGATCAT TGAAATCAAT GGGGAGAGCA CAAGAGACAT GACCCATGCC
    AGAGCAATAG AGCTCATCAA ATCAGGCGGA AGGAGAGTGA GGCTACTGCT GAAGCGTGGG
    ACGGGACAGG TCCCAGAGTA TGACGAACCC GGCTCCTGGA GTGCTCCCGC TGCCGCCTCC
    CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG GAGGACGTCC TCTCTCCATT CTCTCCATCA
    CATCCAGCCC CACCCTCCGA CCCTTCCCAC CAGACAAACC CAGACCCAAC TTGGGATATC
    AAAAGGGAAC ACGACGTTAG GAAACCAAAG GAGCTTTCAG CCGGCAGCCA GAAGAAGCAG
    CGCCTTGGGG AGCAGAGCGA GCGCTCAGCC AGCCCGCAAA GGGCCGTGCA ACCAGGGCTC
    GAGGAGGCGC CTGGCGGCCA GGGGCAGCCT GAGGCCGGCA GGCCCTCCTC GGAGACCAGA
    GCGCCTGGGC CCGCACTGGC CGAAGCGGCG CGGGCTGGCA ACAAGGAGGG GCTGCAGGCC
    CCGGTGGGCT CCGAGCTCGG CCGGCACGGA GGCCCCGGCG CGGCACCTGC GTTCGTGGGC
    CCGGGCCGCG GCGGGGGCGC ACGGGAGGTC GAGGGCCGGG CCGGGGCGCG GGCGGGGTCC
    AGGCAGGGCT CGCGGCCCCC CGGGGGCGCC CCTGCGCGCA AGGCCGCCGT GGCGCCCGGG
    CCGTGGAAGG TGCCGGGCTC CGACAAGCTG CCGGGCGTCC TCAAACCCGG CGCCTCAGCC
    CCGGGGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89709
    Clone ID Related Accession (Same CDS sequence) XM_015468992.1 , XM_015463008.1
    Accession Version XM_015468992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTCC AGATTATAAG
    GAGCTGGATG TTCACCTTCG CAGGATGGAG TCTGGATTTG GCTTCAGAAT CCTCGGGGGA
    GATGAGCCTG GACAGCCTAT TTTGATTGGA GCCGTCATCG CCATGGGCTC AGCTGACAGA
    GATGGCCGCC TACACCCAGG AGATGAGCTC GTCTATGTGG ATGGAATTCC AGTGGCTGGA
    AAGACCCACC GCTATGTCAT TGACCTTATG CACCATGCAG CCCGGAATGG GCAGGTCAAC
    CTCACTGTGA GAAGAAAGGT GCTATGTGGA GGGGAGCCCT GCCCAGAGAA TGGGAGGAGT
    CCAGGCTCCG TGTCGACCCA CCACAGTTCT CCTCGCAGTG ACTACGCAAC CTACACCAAC
    ACCTACAGCA ACAGTAACCA TGCCGTCCCC ACCAGCAACG CCTCTCCCCC CGAAGGCTTC
    GCCTCCCACA GCCTGCAGAC CAGCGACGTG GTCATTCACC GCAAAGAGAA CGAGGGTTTC
    GGCTTTGTCA TCATCAGCTC CCTGAACAGG CCAGAGTCTG GATCCACTAT AACTGTGCCA
    CATAAAATTG GACGCATCAT TGATGGGAGT CCCGCAGATC GCTGTGCAAA GCTAAAAGTG
    GGAGACCGGA TCTTAGCGGT GAACGGCCAG TCTATCATCA ACATGCCTCA TGCAGACATC
    GTGAAGCTCA TCAAGGATGC GGGTCTTAGC GTCACCCTTC GCATCATCCC TCAGGAGGAA
    CTCAACAGCC CCGCTTCAGC ACCCAGCTCA GAGAAGCAGA GCCCCCTGGC CCAGCAGCAC
    AGCCCCCTGG CCCAACAGAG TCCTCTGGCC CAGCCCAGCC CGGCCACGCC CAACAGTCCC
    ATCGCCCAGC CAGCTCCGCC TCAGCCTCTT CAGCCTCAAG GACATGAAAA TAGTTACAGG
    TCAGAAGTAA AAGCGAGGCA AGATGTGAAA CCAGACATCC GACAGCCTCC ATTCACGGAC
    TACAGGCAGC CTCCACTGGA CTACAGGCAA CCCCCAGGAG GGGACTACCC GCAGCCCCCA
    CCCTTGGACT ACAGGCAGCC TCCGCTACTG GACTACAGGC AGCACTCCCC AGACACCAGG
    CAGTTCCCTC TGTCAGACTA CAGGCAGCCC CAGGATTTTG ATTATTTCAC TGTGGACATG
    GAGAAAGGAG CCAAAGGATT TGGATTCAGC ATTCGTGGAG GAAGGGAGTA CAAAATGGAT
    TTGTATGTGC TGAGATTAGC AGAAGATGGG CCAGCAATAA GGAATGGAAG GATGAGGGTA
    GGAGATCAGA TCATTGAAAT CAATGGGGAG AGCACAAGAG ACATGACCCA TGCCAGAGCA
    ATAGAGCTCA TCAAATCAGG CGGAAGGAGA GTGAGGCTAC TGCTGAAGCG TGGGACGGGA
    CAGGTCCCAG AGTATGACGA ACCCGGCTCC TGGAGTGCTC CCGCTGCCGC CTCCCCAGGT
    CTGCCGGAAG TAGGCGTCTC CCTGGAGGAC GTCCTCTCTC CATTCTCTCC ATCACATCCA
    GCCCCACCCT CCGACCCTTC CCACCAGACA AACCCAGACC CAACTTGGGA TATCAAAAGG
    GAACACGACG TTAGGAAACC AAAGGAGCTT TCAGCCGGCA GCCAGAAGAA GCAGCGCCTT
    GGGGAGCAGA GCGAGCGCTC AGCCAGCCCG CAAAGGGCCG TGCAACCAGG GCTCGAGGAG
    GCGCCTGGCG GCCAGGGGCA GCCTGAGGCC GGCAGGCCCT CCTCGGAGAC CAGAGCGCCT
    GGGCCCGCAC TGGCCGAAGC GGCGCGGGCT GGCAACAAGG AGGGGCTGCA GGCCCCGGTG
    GGCTCCGAGC TCGGCCGGCA CGGAGGCCCC GGCGCGGCAC CTGCGTTCGT GGGCCCGGGC
    CGCGGCGGGG GCGCACGGGA GGTCGAGGGC CGGGCCGGGG CGCGGGCGGG GTCCAGGCAG
    GGCTCGCGGC CCCCCGGGGG CGCCCCTGCG CGCAAGGCCG CCGTGGCGCC CGGGCCGTGG
    AAGGTGCCGG GCTCCGACAA GCTGCCGGGC GTCCTCAAAC CCGGCGCCTC AGCCCCGGGG
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324478.1
    CDS919..4884
    Translation

    Target ORF information:

    RefSeq Version XM_015468992.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTCC AGATTATAAG
    GAGCTGGATG TTCACCTTCG CAGGATGGAG TCTGGATTTG GCTTCAGAAT CCTCGGGGGA
    GATGAGCCTG GACAGCCTAT TTTGATTGGA GCCGTCATCG CCATGGGCTC AGCTGACAGA
    GATGGCCGCC TACACCCAGG AGATGAGCTC GTCTATGTGG ATGGAATTCC AGTGGCTGGA
    AAGACCCACC GCTATGTCAT TGACCTTATG CACCATGCAG CCCGGAATGG GCAGGTCAAC
    CTCACTGTGA GAAGAAAGGT GCTATGTGGA GGGGAGCCCT GCCCAGAGAA TGGGAGGAGT
    CCAGGCTCCG TGTCGACCCA CCACAGTTCT CCTCGCAGTG ACTACGCAAC CTACACCAAC
    ACCTACAGCA ACAGTAACCA TGCCGTCCCC ACCAGCAACG CCTCTCCCCC CGAAGGCTTC
    GCCTCCCACA GCCTGCAGAC CAGCGACGTG GTCATTCACC GCAAAGAGAA CGAGGGTTTC
    GGCTTTGTCA TCATCAGCTC CCTGAACAGG CCAGAGTCTG GATCCACTAT AACTGTGCCA
    CATAAAATTG GACGCATCAT TGATGGGAGT CCCGCAGATC GCTGTGCAAA GCTAAAAGTG
    GGAGACCGGA TCTTAGCGGT GAACGGCCAG TCTATCATCA ACATGCCTCA TGCAGACATC
    GTGAAGCTCA TCAAGGATGC GGGTCTTAGC GTCACCCTTC GCATCATCCC TCAGGAGGAA
    CTCAACAGCC CCGCTTCAGC ACCCAGCTCA GAGAAGCAGA GCCCCCTGGC CCAGCAGCAC
    AGCCCCCTGG CCCAACAGAG TCCTCTGGCC CAGCCCAGCC CGGCCACGCC CAACAGTCCC
    ATCGCCCAGC CAGCTCCGCC TCAGCCTCTT CAGCCTCAAG GACATGAAAA TAGTTACAGG
    TCAGAAGTAA AAGCGAGGCA AGATGTGAAA CCAGACATCC GACAGCCTCC ATTCACGGAC
    TACAGGCAGC CTCCACTGGA CTACAGGCAA CCCCCAGGAG GGGACTACCC GCAGCCCCCA
    CCCTTGGACT ACAGGCAGCC TCCGCTACTG GACTACAGGC AGCACTCCCC AGACACCAGG
    CAGTTCCCTC TGTCAGACTA CAGGCAGCCC CAGGATTTTG ATTATTTCAC TGTGGACATG
    GAGAAAGGAG CCAAAGGATT TGGATTCAGC ATTCGTGGAG GAAGGGAGTA CAAAATGGAT
    TTGTATGTGC TGAGATTAGC AGAAGATGGG CCAGCAATAA GGAATGGAAG GATGAGGGTA
    GGAGATCAGA TCATTGAAAT CAATGGGGAG AGCACAAGAG ACATGACCCA TGCCAGAGCA
    ATAGAGCTCA TCAAATCAGG CGGAAGGAGA GTGAGGCTAC TGCTGAAGCG TGGGACGGGA
    CAGGTCCCAG AGTATGACGA ACCCGGCTCC TGGAGTGCTC CCGCTGCCGC CTCCCCAGGT
    CTGCCGGAAG TAGGCGTCTC CCTGGAGGAC GTCCTCTCTC CATTCTCTCC ATCACATCCA
    GCCCCACCCT CCGACCCTTC CCACCAGACA AACCCAGACC CAACTTGGGA TATCAAAAGG
    GAACACGACG TTAGGAAACC AAAGGAGCTT TCAGCCGGCA GCCAGAAGAA GCAGCGCCTT
    GGGGAGCAGA GCGAGCGCTC AGCCAGCCCG CAAAGGGCCG TGCAACCAGG GCTCGAGGAG
    GCGCCTGGCG GCCAGGGGCA GCCTGAGGCC GGCAGGCCCT CCTCGGAGAC CAGAGCGCCT
    GGGCCCGCAC TGGCCGAAGC GGCGCGGGCT GGCAACAAGG AGGGGCTGCA GGCCCCGGTG
    GGCTCCGAGC TCGGCCGGCA CGGAGGCCCC GGCGCGGCAC CTGCGTTCGT GGGCCCGGGC
    CGCGGCGGGG GCGCACGGGA GGTCGAGGGC CGGGCCGGGG CGCGGGCGGG GTCCAGGCAG
    GGCTCGCGGC CCCCCGGGGG CGCCCCTGCG CGCAAGGCCG CCGTGGCGCC CGGGCCGTGG
    AAGGTGCCGG GCTCCGACAA GCTGCCGGGC GTCCTCAAAC CCGGCGCCTC AGCCCCGGGG
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89709
    Clone ID Related Accession (Same CDS sequence) XM_015468992.1 , XM_015463008.1
    Accession Version XM_015463008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTCC AGATTATAAG
    GAGCTGGATG TTCACCTTCG CAGGATGGAG TCTGGATTTG GCTTCAGAAT CCTCGGGGGA
    GATGAGCCTG GACAGCCTAT TTTGATTGGA GCCGTCATCG CCATGGGCTC AGCTGACAGA
    GATGGCCGCC TACACCCAGG AGATGAGCTC GTCTATGTGG ATGGAATTCC AGTGGCTGGA
    AAGACCCACC GCTATGTCAT TGACCTTATG CACCATGCAG CCCGGAATGG GCAGGTCAAC
    CTCACTGTGA GAAGAAAGGT GCTATGTGGA GGGGAGCCCT GCCCAGAGAA TGGGAGGAGT
    CCAGGCTCCG TGTCGACCCA CCACAGTTCT CCTCGCAGTG ACTACGCAAC CTACACCAAC
    ACCTACAGCA ACAGTAACCA TGCCGTCCCC ACCAGCAACG CCTCTCCCCC CGAAGGCTTC
    GCCTCCCACA GCCTGCAGAC CAGCGACGTG GTCATTCACC GCAAAGAGAA CGAGGGTTTC
    GGCTTTGTCA TCATCAGCTC CCTGAACAGG CCAGAGTCTG GATCCACTAT AACTGTGCCA
    CATAAAATTG GACGCATCAT TGATGGGAGT CCCGCAGATC GCTGTGCAAA GCTAAAAGTG
    GGAGACCGGA TCTTAGCGGT GAACGGCCAG TCTATCATCA ACATGCCTCA TGCAGACATC
    GTGAAGCTCA TCAAGGATGC GGGTCTTAGC GTCACCCTTC GCATCATCCC TCAGGAGGAA
    CTCAACAGCC CCGCTTCAGC ACCCAGCTCA GAGAAGCAGA GCCCCCTGGC CCAGCAGCAC
    AGCCCCCTGG CCCAACAGAG TCCTCTGGCC CAGCCCAGCC CGGCCACGCC CAACAGTCCC
    ATCGCCCAGC CAGCTCCGCC TCAGCCTCTT CAGCCTCAAG GACATGAAAA TAGTTACAGG
    TCAGAAGTAA AAGCGAGGCA AGATGTGAAA CCAGACATCC GACAGCCTCC ATTCACGGAC
    TACAGGCAGC CTCCACTGGA CTACAGGCAA CCCCCAGGAG GGGACTACCC GCAGCCCCCA
    CCCTTGGACT ACAGGCAGCC TCCGCTACTG GACTACAGGC AGCACTCCCC AGACACCAGG
    CAGTTCCCTC TGTCAGACTA CAGGCAGCCC CAGGATTTTG ATTATTTCAC TGTGGACATG
    GAGAAAGGAG CCAAAGGATT TGGATTCAGC ATTCGTGGAG GAAGGGAGTA CAAAATGGAT
    TTGTATGTGC TGAGATTAGC AGAAGATGGG CCAGCAATAA GGAATGGAAG GATGAGGGTA
    GGAGATCAGA TCATTGAAAT CAATGGGGAG AGCACAAGAG ACATGACCCA TGCCAGAGCA
    ATAGAGCTCA TCAAATCAGG CGGAAGGAGA GTGAGGCTAC TGCTGAAGCG TGGGACGGGA
    CAGGTCCCAG AGTATGACGA ACCCGGCTCC TGGAGTGCTC CCGCTGCCGC CTCCCCAGGT
    CTGCCGGAAG TAGGCGTCTC CCTGGAGGAC GTCCTCTCTC CATTCTCTCC ATCACATCCA
    GCCCCACCCT CCGACCCTTC CCACCAGACA AACCCAGACC CAACTTGGGA TATCAAAAGG
    GAACACGACG TTAGGAAACC AAAGGAGCTT TCAGCCGGCA GCCAGAAGAA GCAGCGCCTT
    GGGGAGCAGA GCGAGCGCTC AGCCAGCCCG CAAAGGGCCG TGCAACCAGG GCTCGAGGAG
    GCGCCTGGCG GCCAGGGGCA GCCTGAGGCC GGCAGGCCCT CCTCGGAGAC CAGAGCGCCT
    GGGCCCGCAC TGGCCGAAGC GGCGCGGGCT GGCAACAAGG AGGGGCTGCA GGCCCCGGTG
    GGCTCCGAGC TCGGCCGGCA CGGAGGCCCC GGCGCGGCAC CTGCGTTCGT GGGCCCGGGC
    CGCGGCGGGG GCGCACGGGA GGTCGAGGGC CGGGCCGGGG CGCGGGCGGG GTCCAGGCAG
    GGCTCGCGGC CCCCCGGGGG CGCCCCTGCG CGCAAGGCCG CCGTGGCGCC CGGGCCGTGG
    AAGGTGCCGG GCTCCGACAA GCTGCCGGGC GTCCTCAAAC CCGGCGCCTC AGCCCCGGGG
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318494.1
    CDS919..4884
    Translation

    Target ORF information:

    RefSeq Version XM_015463008.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTCC AGATTATAAG
    GAGCTGGATG TTCACCTTCG CAGGATGGAG TCTGGATTTG GCTTCAGAAT CCTCGGGGGA
    GATGAGCCTG GACAGCCTAT TTTGATTGGA GCCGTCATCG CCATGGGCTC AGCTGACAGA
    GATGGCCGCC TACACCCAGG AGATGAGCTC GTCTATGTGG ATGGAATTCC AGTGGCTGGA
    AAGACCCACC GCTATGTCAT TGACCTTATG CACCATGCAG CCCGGAATGG GCAGGTCAAC
    CTCACTGTGA GAAGAAAGGT GCTATGTGGA GGGGAGCCCT GCCCAGAGAA TGGGAGGAGT
    CCAGGCTCCG TGTCGACCCA CCACAGTTCT CCTCGCAGTG ACTACGCAAC CTACACCAAC
    ACCTACAGCA ACAGTAACCA TGCCGTCCCC ACCAGCAACG CCTCTCCCCC CGAAGGCTTC
    GCCTCCCACA GCCTGCAGAC CAGCGACGTG GTCATTCACC GCAAAGAGAA CGAGGGTTTC
    GGCTTTGTCA TCATCAGCTC CCTGAACAGG CCAGAGTCTG GATCCACTAT AACTGTGCCA
    CATAAAATTG GACGCATCAT TGATGGGAGT CCCGCAGATC GCTGTGCAAA GCTAAAAGTG
    GGAGACCGGA TCTTAGCGGT GAACGGCCAG TCTATCATCA ACATGCCTCA TGCAGACATC
    GTGAAGCTCA TCAAGGATGC GGGTCTTAGC GTCACCCTTC GCATCATCCC TCAGGAGGAA
    CTCAACAGCC CCGCTTCAGC ACCCAGCTCA GAGAAGCAGA GCCCCCTGGC CCAGCAGCAC
    AGCCCCCTGG CCCAACAGAG TCCTCTGGCC CAGCCCAGCC CGGCCACGCC CAACAGTCCC
    ATCGCCCAGC CAGCTCCGCC TCAGCCTCTT CAGCCTCAAG GACATGAAAA TAGTTACAGG
    TCAGAAGTAA AAGCGAGGCA AGATGTGAAA CCAGACATCC GACAGCCTCC ATTCACGGAC
    TACAGGCAGC CTCCACTGGA CTACAGGCAA CCCCCAGGAG GGGACTACCC GCAGCCCCCA
    CCCTTGGACT ACAGGCAGCC TCCGCTACTG GACTACAGGC AGCACTCCCC AGACACCAGG
    CAGTTCCCTC TGTCAGACTA CAGGCAGCCC CAGGATTTTG ATTATTTCAC TGTGGACATG
    GAGAAAGGAG CCAAAGGATT TGGATTCAGC ATTCGTGGAG GAAGGGAGTA CAAAATGGAT
    TTGTATGTGC TGAGATTAGC AGAAGATGGG CCAGCAATAA GGAATGGAAG GATGAGGGTA
    GGAGATCAGA TCATTGAAAT CAATGGGGAG AGCACAAGAG ACATGACCCA TGCCAGAGCA
    ATAGAGCTCA TCAAATCAGG CGGAAGGAGA GTGAGGCTAC TGCTGAAGCG TGGGACGGGA
    CAGGTCCCAG AGTATGACGA ACCCGGCTCC TGGAGTGCTC CCGCTGCCGC CTCCCCAGGT
    CTGCCGGAAG TAGGCGTCTC CCTGGAGGAC GTCCTCTCTC CATTCTCTCC ATCACATCCA
    GCCCCACCCT CCGACCCTTC CCACCAGACA AACCCAGACC CAACTTGGGA TATCAAAAGG
    GAACACGACG TTAGGAAACC AAAGGAGCTT TCAGCCGGCA GCCAGAAGAA GCAGCGCCTT
    GGGGAGCAGA GCGAGCGCTC AGCCAGCCCG CAAAGGGCCG TGCAACCAGG GCTCGAGGAG
    GCGCCTGGCG GCCAGGGGCA GCCTGAGGCC GGCAGGCCCT CCTCGGAGAC CAGAGCGCCT
    GGGCCCGCAC TGGCCGAAGC GGCGCGGGCT GGCAACAAGG AGGGGCTGCA GGCCCCGGTG
    GGCTCCGAGC TCGGCCGGCA CGGAGGCCCC GGCGCGGCAC CTGCGTTCGT GGGCCCGGGC
    CGCGGCGGGG GCGCACGGGA GGTCGAGGGC CGGGCCGGGG CGCGGGCGGG GTCCAGGCAG
    GGCTCGCGGC CCCCCGGGGG CGCCCCTGCG CGCAAGGCCG CCGTGGCGCC CGGGCCGTGG
    AAGGTGCCGG GCTCCGACAA GCTGCCGGGC GTCCTCAAAC CCGGCGCCTC AGCCCCGGGG
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89710
    Clone ID Related Accession (Same CDS sequence) XM_015468993.1 , XM_015463009.1
    Accession Version XM_015468993.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA AAAGCCACTC TTTACCCGGG ATGCCTCCCA ATTGAAGGGA
    ACGTTTCTCA GCACCACACT CAAAAAGAGC AACATGGGGT TTGGATTTAC CATTATTGGC
    GGAGATGAGC CTGACGAGTT TCTGCAGGTG AAAAGTGTGA TTCCCGACGG GCCTGCAGCA
    CAAGACGGGA AAATGGAAAC AGGTGATGTC ATTGTCTATA TTAATGAAGT TTGTGTCCTT
    GGACACACTC ATGCAGATGT AGTCAAACTT TTCCAGTCTG TTCCTATTGG TCAGAGTGTC
    AACTTGGTGT TGTGTCGTGG CTACCCTTTG CCCTTTGATC CTGAAGATCC TGCTAACAGC
    ATGGTGCCAC CCCTTGCAAT AATGGAGAGG CCACCTCCAG TGATGGTCAA TGGAAGACAT
    AACTATGAAA CATATTTGGA ATACATTTCT CGGACCTCAC AGTCAGTTCC GGATATAACA
    GATCGGCCGC CTCATACTTT GCACTCAATG CCAGCTGATG GTCAGCTAGA TGGCACGTAT
    CCACCACCTG TCCATGACGA CAATGTATCT ATGGCTTCAT CTGGGGCCAC CCAAGCTGAA
    CTTATGACCT TAACCATTGT GAAAGGTGCC CAGGGTTTTG GCTTCACCAT TGCAGACAGT
    CCTACAGGAC AGCGGGTGAA ACAAATACTT GACATTCAGG GATGCCCTGG CCTGTGTGAA
    GGCGACCTCA TTATTGAGAT CAACCAGCAG AATGTACAGA ACCTGAGCCA TACGGAAGTA
    GTGGATATAC TTAAGGACTG TCCCATTGGA AGCGAGACTT CTTTGATTAT TCATCGAGGA
    GGTTTCTTTT CTCCATGGAA AACCCCAAAG CCTATAATGG ATCGATGGGA GAACCAAGGC
    AGTCCTCAAA CGAGTTTATC TGCTCCGGCC ATACCACAGA ACCTGCCCTT CCCACCCGCC
    CTTCACAGGA GTTCCTTTCC TGACTCAACA GAGGCCTTTG ACCCAAGGAA GCCTGACCCA
    TATGAGCTCT ACGAGAAATC GAGAGCCATT TATGAAAGTA GGCAACAAGT GCCACCAAGG
    ACCAGTTTTC GAATGGATTC TTCTGGTCCA GATTATAAGG AGCTGGATGT TCACCTTCGC
    AGGATGGAGT CTGGATTTGG CTTCAGAATC CTCGGGGGAG ATGAGCCTGG ACAGCCTATT
    TTGATTGGAG CCGTCATCGC CATGGGCTCA GCTGACAGAG ATGGCCGCCT ACACCCAGGA
    GATGAGCTCG TCTATGTGGA TGGAATTCCA GTGGCTGGAA AGACCCACCG CTATGTCATT
    GACCTTATGC ACCATGCAGC CCGGAATGGG CAGGTCAACC TCACTGTGAG AAGAAAGGTG
    CTATGTGGAG GGGAGCCCTG CCCAGAGAAT GGGAGGAGTC CAGGCTCCGT GTCGACCCAC
    CACAGTTCTC CTCGCAGTGA CTACGCAACC TACACCAACA CCTACAGCAA CAGTAACCAT
    GCCGTCCCCA CCAGCAACGC CTCTCCCCCC GAAGGCTTCG CCTCCCACAG CCTGCAGACC
    AGCGACGTGG TCATTCACCG CAAAGAGAAC GAGGGTTTCG GCTTTGTCAT CATCAGCTCC
    CTGAACAGGC CAGAGTCTGG ATCCACTATA ACTGTGCCAC ATAAAATTGG ACGCATCATT
    GATGGGAGTC CCGCAGATCG CTGTGCAAAG CTAAAAGTGG GAGACCGGAT CTTAGCGGTG
    AACGGCCAGT CTATCATCAA CATGCCTCAT GCAGACATCG TGAAGCTCAT CAAGGATGCG
    GGTCTTAGCG TCACCCTTCG CATCATCCCT CAGGAGGAAC TCAACAGCCC CGCTTCAGCA
    CCCAGCTCAG AGAAGCAGAG CCCCCTGGCC CAGCAGCACA GCCCCCTGGC CCAACAGAGT
    CCTCTGGCCC AGCCCAGCCC GGCCACGCCC AACAGTCCCA TCGCCCAGCC AGCTCCGCCT
    CAGCCTCTTC AGCCTCAAGG ACATGAAAAT AGTTACAGGT CAGAAGTAAA AGCGAGGCAA
    GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACGGACT ACAGGCAGCC TCCACTGGAC
    TACAGGCAAC CCCCAGGAGG GGACTACCCG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
    CCGCTACTGG ACTACAGGCA GCACTCCCCA GACACCAGGC AGTTCCCTCT GTCAGACTAC
    AGGCAGCCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
    GGATTCAGCA TTCGTGGAGG AAGGGAGTAC AAAATGGATT TGTATGTGCT GAGATTAGCA
    GAAGATGGGC CAGCAATAAG GAATGGAAGG ATGAGGGTAG GAGATCAGAT CATTGAAATC
    AATGGGGAGA GCACAAGAGA CATGACCCAT GCCAGAGCAA TAGAGCTCAT CAAATCAGGC
    GGAAGGAGAG TGAGGCTACT GCTGAAGCGT GGGACGGGAC AGGTCCCAGA GTATGACGAA
    CCCGGCTCCT GGAGTGCTCC CGCTGCCGCC TCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
    CTGGAGGACG TCCTCTCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
    CACCAGACAA ACCCAGACCC AACTTGGGAT ATCAAAAGGG AACACGACGT TAGGAAACCA
    AAGGAGCTTT CAGCCGGCAG CCAGAAGAAG CAGCGCCTTG GGGAGCAGAG CGAGCGCTCA
    GCCAGCCCGC AAAGGGCCGT GCAACCAGGG CTCGAGGAGG CGCCTGGCGG CCAGGGGCAG
    CCTGAGGCCG GCAGGCCCTC CTCGGAGACC AGAGCGCCTG GGCCCGCACT GGCCGAAGCG
    GCGCGGGCTG GCAACAAGGA GGGGCTGCAG GCCCCGGTGG GCTCCGAGCT CGGCCGGCAC
    GGAGGCCCCG GCGCGGCACC TGCGTTCGTG GGCCCGGGCC GCGGCGGGGG CGCACGGGAG
    GTCGAGGGCC GGGCCGGGGC GCGGGCGGGG TCCAGGCAGG GCTCGCGGCC CCCCGGGGGC
    GCCCCTGCGC GCAAGGCCGC CGTGGCGCCC GGGCCGTGGA AGGTGCCGGG CTCCGACAAG
    CTGCCGGGCG TCCTCAAACC CGGCGCCTCA GCCCCGGGGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324479.1
    CDS916..4800
    Translation

    Target ORF information:

    RefSeq Version XM_015468993.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468993.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA AAAGCCACTC TTTACCCGGG ATGCCTCCCA ATTGAAGGGA
    ACGTTTCTCA GCACCACACT CAAAAAGAGC AACATGGGGT TTGGATTTAC CATTATTGGC
    GGAGATGAGC CTGACGAGTT TCTGCAGGTG AAAAGTGTGA TTCCCGACGG GCCTGCAGCA
    CAAGACGGGA AAATGGAAAC AGGTGATGTC ATTGTCTATA TTAATGAAGT TTGTGTCCTT
    GGACACACTC ATGCAGATGT AGTCAAACTT TTCCAGTCTG TTCCTATTGG TCAGAGTGTC
    AACTTGGTGT TGTGTCGTGG CTACCCTTTG CCCTTTGATC CTGAAGATCC TGCTAACAGC
    ATGGTGCCAC CCCTTGCAAT AATGGAGAGG CCACCTCCAG TGATGGTCAA TGGAAGACAT
    AACTATGAAA CATATTTGGA ATACATTTCT CGGACCTCAC AGTCAGTTCC GGATATAACA
    GATCGGCCGC CTCATACTTT GCACTCAATG CCAGCTGATG GTCAGCTAGA TGGCACGTAT
    CCACCACCTG TCCATGACGA CAATGTATCT ATGGCTTCAT CTGGGGCCAC CCAAGCTGAA
    CTTATGACCT TAACCATTGT GAAAGGTGCC CAGGGTTTTG GCTTCACCAT TGCAGACAGT
    CCTACAGGAC AGCGGGTGAA ACAAATACTT GACATTCAGG GATGCCCTGG CCTGTGTGAA
    GGCGACCTCA TTATTGAGAT CAACCAGCAG AATGTACAGA ACCTGAGCCA TACGGAAGTA
    GTGGATATAC TTAAGGACTG TCCCATTGGA AGCGAGACTT CTTTGATTAT TCATCGAGGA
    GGTTTCTTTT CTCCATGGAA AACCCCAAAG CCTATAATGG ATCGATGGGA GAACCAAGGC
    AGTCCTCAAA CGAGTTTATC TGCTCCGGCC ATACCACAGA ACCTGCCCTT CCCACCCGCC
    CTTCACAGGA GTTCCTTTCC TGACTCAACA GAGGCCTTTG ACCCAAGGAA GCCTGACCCA
    TATGAGCTCT ACGAGAAATC GAGAGCCATT TATGAAAGTA GGCAACAAGT GCCACCAAGG
    ACCAGTTTTC GAATGGATTC TTCTGGTCCA GATTATAAGG AGCTGGATGT TCACCTTCGC
    AGGATGGAGT CTGGATTTGG CTTCAGAATC CTCGGGGGAG ATGAGCCTGG ACAGCCTATT
    TTGATTGGAG CCGTCATCGC CATGGGCTCA GCTGACAGAG ATGGCCGCCT ACACCCAGGA
    GATGAGCTCG TCTATGTGGA TGGAATTCCA GTGGCTGGAA AGACCCACCG CTATGTCATT
    GACCTTATGC ACCATGCAGC CCGGAATGGG CAGGTCAACC TCACTGTGAG AAGAAAGGTG
    CTATGTGGAG GGGAGCCCTG CCCAGAGAAT GGGAGGAGTC CAGGCTCCGT GTCGACCCAC
    CACAGTTCTC CTCGCAGTGA CTACGCAACC TACACCAACA CCTACAGCAA CAGTAACCAT
    GCCGTCCCCA CCAGCAACGC CTCTCCCCCC GAAGGCTTCG CCTCCCACAG CCTGCAGACC
    AGCGACGTGG TCATTCACCG CAAAGAGAAC GAGGGTTTCG GCTTTGTCAT CATCAGCTCC
    CTGAACAGGC CAGAGTCTGG ATCCACTATA ACTGTGCCAC ATAAAATTGG ACGCATCATT
    GATGGGAGTC CCGCAGATCG CTGTGCAAAG CTAAAAGTGG GAGACCGGAT CTTAGCGGTG
    AACGGCCAGT CTATCATCAA CATGCCTCAT GCAGACATCG TGAAGCTCAT CAAGGATGCG
    GGTCTTAGCG TCACCCTTCG CATCATCCCT CAGGAGGAAC TCAACAGCCC CGCTTCAGCA
    CCCAGCTCAG AGAAGCAGAG CCCCCTGGCC CAGCAGCACA GCCCCCTGGC CCAACAGAGT
    CCTCTGGCCC AGCCCAGCCC GGCCACGCCC AACAGTCCCA TCGCCCAGCC AGCTCCGCCT
    CAGCCTCTTC AGCCTCAAGG ACATGAAAAT AGTTACAGGT CAGAAGTAAA AGCGAGGCAA
    GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACGGACT ACAGGCAGCC TCCACTGGAC
    TACAGGCAAC CCCCAGGAGG GGACTACCCG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
    CCGCTACTGG ACTACAGGCA GCACTCCCCA GACACCAGGC AGTTCCCTCT GTCAGACTAC
    AGGCAGCCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
    GGATTCAGCA TTCGTGGAGG AAGGGAGTAC AAAATGGATT TGTATGTGCT GAGATTAGCA
    GAAGATGGGC CAGCAATAAG GAATGGAAGG ATGAGGGTAG GAGATCAGAT CATTGAAATC
    AATGGGGAGA GCACAAGAGA CATGACCCAT GCCAGAGCAA TAGAGCTCAT CAAATCAGGC
    GGAAGGAGAG TGAGGCTACT GCTGAAGCGT GGGACGGGAC AGGTCCCAGA GTATGACGAA
    CCCGGCTCCT GGAGTGCTCC CGCTGCCGCC TCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
    CTGGAGGACG TCCTCTCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
    CACCAGACAA ACCCAGACCC AACTTGGGAT ATCAAAAGGG AACACGACGT TAGGAAACCA
    AAGGAGCTTT CAGCCGGCAG CCAGAAGAAG CAGCGCCTTG GGGAGCAGAG CGAGCGCTCA
    GCCAGCCCGC AAAGGGCCGT GCAACCAGGG CTCGAGGAGG CGCCTGGCGG CCAGGGGCAG
    CCTGAGGCCG GCAGGCCCTC CTCGGAGACC AGAGCGCCTG GGCCCGCACT GGCCGAAGCG
    GCGCGGGCTG GCAACAAGGA GGGGCTGCAG GCCCCGGTGG GCTCCGAGCT CGGCCGGCAC
    GGAGGCCCCG GCGCGGCACC TGCGTTCGTG GGCCCGGGCC GCGGCGGGGG CGCACGGGAG
    GTCGAGGGCC GGGCCGGGGC GCGGGCGGGG TCCAGGCAGG GCTCGCGGCC CCCCGGGGGC
    GCCCCTGCGC GCAAGGCCGC CGTGGCGCCC GGGCCGTGGA AGGTGCCGGG CTCCGACAAG
    CTGCCGGGCG TCCTCAAACC CGGCGCCTCA GCCCCGGGGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89710
    Clone ID Related Accession (Same CDS sequence) XM_015468993.1 , XM_015463009.1
    Accession Version XM_015463009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA AAAGCCACTC TTTACCCGGG ATGCCTCCCA ATTGAAGGGA
    ACGTTTCTCA GCACCACACT CAAAAAGAGC AACATGGGGT TTGGATTTAC CATTATTGGC
    GGAGATGAGC CTGACGAGTT TCTGCAGGTG AAAAGTGTGA TTCCCGACGG GCCTGCAGCA
    CAAGACGGGA AAATGGAAAC AGGTGATGTC ATTGTCTATA TTAATGAAGT TTGTGTCCTT
    GGACACACTC ATGCAGATGT AGTCAAACTT TTCCAGTCTG TTCCTATTGG TCAGAGTGTC
    AACTTGGTGT TGTGTCGTGG CTACCCTTTG CCCTTTGATC CTGAAGATCC TGCTAACAGC
    ATGGTGCCAC CCCTTGCAAT AATGGAGAGG CCACCTCCAG TGATGGTCAA TGGAAGACAT
    AACTATGAAA CATATTTGGA ATACATTTCT CGGACCTCAC AGTCAGTTCC GGATATAACA
    GATCGGCCGC CTCATACTTT GCACTCAATG CCAGCTGATG GTCAGCTAGA TGGCACGTAT
    CCACCACCTG TCCATGACGA CAATGTATCT ATGGCTTCAT CTGGGGCCAC CCAAGCTGAA
    CTTATGACCT TAACCATTGT GAAAGGTGCC CAGGGTTTTG GCTTCACCAT TGCAGACAGT
    CCTACAGGAC AGCGGGTGAA ACAAATACTT GACATTCAGG GATGCCCTGG CCTGTGTGAA
    GGCGACCTCA TTATTGAGAT CAACCAGCAG AATGTACAGA ACCTGAGCCA TACGGAAGTA
    GTGGATATAC TTAAGGACTG TCCCATTGGA AGCGAGACTT CTTTGATTAT TCATCGAGGA
    GGTTTCTTTT CTCCATGGAA AACCCCAAAG CCTATAATGG ATCGATGGGA GAACCAAGGC
    AGTCCTCAAA CGAGTTTATC TGCTCCGGCC ATACCACAGA ACCTGCCCTT CCCACCCGCC
    CTTCACAGGA GTTCCTTTCC TGACTCAACA GAGGCCTTTG ACCCAAGGAA GCCTGACCCA
    TATGAGCTCT ACGAGAAATC GAGAGCCATT TATGAAAGTA GGCAACAAGT GCCACCAAGG
    ACCAGTTTTC GAATGGATTC TTCTGGTCCA GATTATAAGG AGCTGGATGT TCACCTTCGC
    AGGATGGAGT CTGGATTTGG CTTCAGAATC CTCGGGGGAG ATGAGCCTGG ACAGCCTATT
    TTGATTGGAG CCGTCATCGC CATGGGCTCA GCTGACAGAG ATGGCCGCCT ACACCCAGGA
    GATGAGCTCG TCTATGTGGA TGGAATTCCA GTGGCTGGAA AGACCCACCG CTATGTCATT
    GACCTTATGC ACCATGCAGC CCGGAATGGG CAGGTCAACC TCACTGTGAG AAGAAAGGTG
    CTATGTGGAG GGGAGCCCTG CCCAGAGAAT GGGAGGAGTC CAGGCTCCGT GTCGACCCAC
    CACAGTTCTC CTCGCAGTGA CTACGCAACC TACACCAACA CCTACAGCAA CAGTAACCAT
    GCCGTCCCCA CCAGCAACGC CTCTCCCCCC GAAGGCTTCG CCTCCCACAG CCTGCAGACC
    AGCGACGTGG TCATTCACCG CAAAGAGAAC GAGGGTTTCG GCTTTGTCAT CATCAGCTCC
    CTGAACAGGC CAGAGTCTGG ATCCACTATA ACTGTGCCAC ATAAAATTGG ACGCATCATT
    GATGGGAGTC CCGCAGATCG CTGTGCAAAG CTAAAAGTGG GAGACCGGAT CTTAGCGGTG
    AACGGCCAGT CTATCATCAA CATGCCTCAT GCAGACATCG TGAAGCTCAT CAAGGATGCG
    GGTCTTAGCG TCACCCTTCG CATCATCCCT CAGGAGGAAC TCAACAGCCC CGCTTCAGCA
    CCCAGCTCAG AGAAGCAGAG CCCCCTGGCC CAGCAGCACA GCCCCCTGGC CCAACAGAGT
    CCTCTGGCCC AGCCCAGCCC GGCCACGCCC AACAGTCCCA TCGCCCAGCC AGCTCCGCCT
    CAGCCTCTTC AGCCTCAAGG ACATGAAAAT AGTTACAGGT CAGAAGTAAA AGCGAGGCAA
    GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACGGACT ACAGGCAGCC TCCACTGGAC
    TACAGGCAAC CCCCAGGAGG GGACTACCCG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
    CCGCTACTGG ACTACAGGCA GCACTCCCCA GACACCAGGC AGTTCCCTCT GTCAGACTAC
    AGGCAGCCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
    GGATTCAGCA TTCGTGGAGG AAGGGAGTAC AAAATGGATT TGTATGTGCT GAGATTAGCA
    GAAGATGGGC CAGCAATAAG GAATGGAAGG ATGAGGGTAG GAGATCAGAT CATTGAAATC
    AATGGGGAGA GCACAAGAGA CATGACCCAT GCCAGAGCAA TAGAGCTCAT CAAATCAGGC
    GGAAGGAGAG TGAGGCTACT GCTGAAGCGT GGGACGGGAC AGGTCCCAGA GTATGACGAA
    CCCGGCTCCT GGAGTGCTCC CGCTGCCGCC TCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
    CTGGAGGACG TCCTCTCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
    CACCAGACAA ACCCAGACCC AACTTGGGAT ATCAAAAGGG AACACGACGT TAGGAAACCA
    AAGGAGCTTT CAGCCGGCAG CCAGAAGAAG CAGCGCCTTG GGGAGCAGAG CGAGCGCTCA
    GCCAGCCCGC AAAGGGCCGT GCAACCAGGG CTCGAGGAGG CGCCTGGCGG CCAGGGGCAG
    CCTGAGGCCG GCAGGCCCTC CTCGGAGACC AGAGCGCCTG GGCCCGCACT GGCCGAAGCG
    GCGCGGGCTG GCAACAAGGA GGGGCTGCAG GCCCCGGTGG GCTCCGAGCT CGGCCGGCAC
    GGAGGCCCCG GCGCGGCACC TGCGTTCGTG GGCCCGGGCC GCGGCGGGGG CGCACGGGAG
    GTCGAGGGCC GGGCCGGGGC GCGGGCGGGG TCCAGGCAGG GCTCGCGGCC CCCCGGGGGC
    GCCCCTGCGC GCAAGGCCGC CGTGGCGCCC GGGCCGTGGA AGGTGCCGGG CTCCGACAAG
    CTGCCGGGCG TCCTCAAACC CGGCGCCTCA GCCCCGGGGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318495.1
    CDS916..4800
    Translation

    Target ORF information:

    RefSeq Version XM_015463009.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA AAAGCCACTC TTTACCCGGG ATGCCTCCCA ATTGAAGGGA
    ACGTTTCTCA GCACCACACT CAAAAAGAGC AACATGGGGT TTGGATTTAC CATTATTGGC
    GGAGATGAGC CTGACGAGTT TCTGCAGGTG AAAAGTGTGA TTCCCGACGG GCCTGCAGCA
    CAAGACGGGA AAATGGAAAC AGGTGATGTC ATTGTCTATA TTAATGAAGT TTGTGTCCTT
    GGACACACTC ATGCAGATGT AGTCAAACTT TTCCAGTCTG TTCCTATTGG TCAGAGTGTC
    AACTTGGTGT TGTGTCGTGG CTACCCTTTG CCCTTTGATC CTGAAGATCC TGCTAACAGC
    ATGGTGCCAC CCCTTGCAAT AATGGAGAGG CCACCTCCAG TGATGGTCAA TGGAAGACAT
    AACTATGAAA CATATTTGGA ATACATTTCT CGGACCTCAC AGTCAGTTCC GGATATAACA
    GATCGGCCGC CTCATACTTT GCACTCAATG CCAGCTGATG GTCAGCTAGA TGGCACGTAT
    CCACCACCTG TCCATGACGA CAATGTATCT ATGGCTTCAT CTGGGGCCAC CCAAGCTGAA
    CTTATGACCT TAACCATTGT GAAAGGTGCC CAGGGTTTTG GCTTCACCAT TGCAGACAGT
    CCTACAGGAC AGCGGGTGAA ACAAATACTT GACATTCAGG GATGCCCTGG CCTGTGTGAA
    GGCGACCTCA TTATTGAGAT CAACCAGCAG AATGTACAGA ACCTGAGCCA TACGGAAGTA
    GTGGATATAC TTAAGGACTG TCCCATTGGA AGCGAGACTT CTTTGATTAT TCATCGAGGA
    GGTTTCTTTT CTCCATGGAA AACCCCAAAG CCTATAATGG ATCGATGGGA GAACCAAGGC
    AGTCCTCAAA CGAGTTTATC TGCTCCGGCC ATACCACAGA ACCTGCCCTT CCCACCCGCC
    CTTCACAGGA GTTCCTTTCC TGACTCAACA GAGGCCTTTG ACCCAAGGAA GCCTGACCCA
    TATGAGCTCT ACGAGAAATC GAGAGCCATT TATGAAAGTA GGCAACAAGT GCCACCAAGG
    ACCAGTTTTC GAATGGATTC TTCTGGTCCA GATTATAAGG AGCTGGATGT TCACCTTCGC
    AGGATGGAGT CTGGATTTGG CTTCAGAATC CTCGGGGGAG ATGAGCCTGG ACAGCCTATT
    TTGATTGGAG CCGTCATCGC CATGGGCTCA GCTGACAGAG ATGGCCGCCT ACACCCAGGA
    GATGAGCTCG TCTATGTGGA TGGAATTCCA GTGGCTGGAA AGACCCACCG CTATGTCATT
    GACCTTATGC ACCATGCAGC CCGGAATGGG CAGGTCAACC TCACTGTGAG AAGAAAGGTG
    CTATGTGGAG GGGAGCCCTG CCCAGAGAAT GGGAGGAGTC CAGGCTCCGT GTCGACCCAC
    CACAGTTCTC CTCGCAGTGA CTACGCAACC TACACCAACA CCTACAGCAA CAGTAACCAT
    GCCGTCCCCA CCAGCAACGC CTCTCCCCCC GAAGGCTTCG CCTCCCACAG CCTGCAGACC
    AGCGACGTGG TCATTCACCG CAAAGAGAAC GAGGGTTTCG GCTTTGTCAT CATCAGCTCC
    CTGAACAGGC CAGAGTCTGG ATCCACTATA ACTGTGCCAC ATAAAATTGG ACGCATCATT
    GATGGGAGTC CCGCAGATCG CTGTGCAAAG CTAAAAGTGG GAGACCGGAT CTTAGCGGTG
    AACGGCCAGT CTATCATCAA CATGCCTCAT GCAGACATCG TGAAGCTCAT CAAGGATGCG
    GGTCTTAGCG TCACCCTTCG CATCATCCCT CAGGAGGAAC TCAACAGCCC CGCTTCAGCA
    CCCAGCTCAG AGAAGCAGAG CCCCCTGGCC CAGCAGCACA GCCCCCTGGC CCAACAGAGT
    CCTCTGGCCC AGCCCAGCCC GGCCACGCCC AACAGTCCCA TCGCCCAGCC AGCTCCGCCT
    CAGCCTCTTC AGCCTCAAGG ACATGAAAAT AGTTACAGGT CAGAAGTAAA AGCGAGGCAA
    GATGTGAAAC CAGACATCCG ACAGCCTCCA TTCACGGACT ACAGGCAGCC TCCACTGGAC
    TACAGGCAAC CCCCAGGAGG GGACTACCCG CAGCCCCCAC CCTTGGACTA CAGGCAGCCT
    CCGCTACTGG ACTACAGGCA GCACTCCCCA GACACCAGGC AGTTCCCTCT GTCAGACTAC
    AGGCAGCCCC AGGATTTTGA TTATTTCACT GTGGACATGG AGAAAGGAGC CAAAGGATTT
    GGATTCAGCA TTCGTGGAGG AAGGGAGTAC AAAATGGATT TGTATGTGCT GAGATTAGCA
    GAAGATGGGC CAGCAATAAG GAATGGAAGG ATGAGGGTAG GAGATCAGAT CATTGAAATC
    AATGGGGAGA GCACAAGAGA CATGACCCAT GCCAGAGCAA TAGAGCTCAT CAAATCAGGC
    GGAAGGAGAG TGAGGCTACT GCTGAAGCGT GGGACGGGAC AGGTCCCAGA GTATGACGAA
    CCCGGCTCCT GGAGTGCTCC CGCTGCCGCC TCCCCAGGTC TGCCGGAAGT AGGCGTCTCC
    CTGGAGGACG TCCTCTCTCC ATTCTCTCCA TCACATCCAG CCCCACCCTC CGACCCTTCC
    CACCAGACAA ACCCAGACCC AACTTGGGAT ATCAAAAGGG AACACGACGT TAGGAAACCA
    AAGGAGCTTT CAGCCGGCAG CCAGAAGAAG CAGCGCCTTG GGGAGCAGAG CGAGCGCTCA
    GCCAGCCCGC AAAGGGCCGT GCAACCAGGG CTCGAGGAGG CGCCTGGCGG CCAGGGGCAG
    CCTGAGGCCG GCAGGCCCTC CTCGGAGACC AGAGCGCCTG GGCCCGCACT GGCCGAAGCG
    GCGCGGGCTG GCAACAAGGA GGGGCTGCAG GCCCCGGTGG GCTCCGAGCT CGGCCGGCAC
    GGAGGCCCCG GCGCGGCACC TGCGTTCGTG GGCCCGGGCC GCGGCGGGGG CGCACGGGAG
    GTCGAGGGCC GGGCCGGGGC GCGGGCGGGG TCCAGGCAGG GCTCGCGGCC CCCCGGGGGC
    GCCCCTGCGC GCAAGGCCGC CGTGGCGCCC GGGCCGTGGA AGGTGCCGGG CTCCGACAAG
    CTGCCGGGCG TCCTCAAACC CGGCGCCTCA GCCCCGGGGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89711
    Clone ID Related Accession (Same CDS sequence) XM_015468994.1 , XM_015463010.1
    Accession Version XM_015468994.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCAAGGACC
    AGTTTTCGAA TGGATTCTTC TGGTCCAGAT TATAAGGAGC TGGATGTTCA CCTTCGCAGG
    ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
    ATTGGAGCCG TCATCGCCAT GGGCTCAGCT GACAGAGATG GCCGCCTACA CCCAGGAGAT
    GAGCTCGTCT ATGTGGATGG AATTCCAGTG GCTGGAAAGA CCCACCGCTA TGTCATTGAC
    CTTATGCACC ATGCAGCCCG GAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
    TGTGGAGGGG AGCCCTGCCC AGAGAATGGG AGGAGTCCAG GCTCCGTGTC GACCCACCAC
    AGTTCTCCTC GCAGTGACTA CGCAACCTAC ACCAACACCT ACAGCAACAG TAACCATGCC
    GTCCCCACCA GCAACGCCTC TCCCCCCGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGC
    GACGTGGTCA TTCACCGCAA AGAGAACGAG GGTTTCGGCT TTGTCATCAT CAGCTCCCTG
    AACAGGCCAG AGTCTGGATC CACTATAACT GTGCCACATA AAATTGGACG CATCATTGAT
    GGGAGTCCCG CAGATCGCTG TGCAAAGCTA AAAGTGGGAG ACCGGATCTT AGCGGTGAAC
    GGCCAGTCTA TCATCAACAT GCCTCATGCA GACATCGTGA AGCTCATCAA GGATGCGGGT
    CTTAGCGTCA CCCTTCGCAT CATCCCTCAG GAGGAACTCA ACAGCCCCGC TTCAGCACCC
    AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG CAGCACAGCC CCCTGGCCCA ACAGAGTCCT
    CTGGCCCAGC CCAGCCCGGC CACGCCCAAC AGTCCCATCG CCCAGCCAGC TCCGCCTCAG
    CCTCTTCAGC CTCAAGGACA TGAAAATAGT TACAGGTCAG AAGTAAAAGC GAGGCAAGAT
    GTGAAACCAG ACATCCGACA GCCTCCATTC ACGGACTACA GGCAGCCTCC ACTGGACTAC
    AGGCAACCCC CAGGAGGGGA CTACCCGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCG
    CTACTGGACT ACAGGCAGCA CTCCCCAGAC ACCAGGCAGT TCCCTCTGTC AGACTACAGG
    CAGCCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
    TTCAGCATTC GTGGAGGAAG GGAGTACAAA ATGGATTTGT ATGTGCTGAG ATTAGCAGAA
    GATGGGCCAG CAATAAGGAA TGGAAGGATG AGGGTAGGAG ATCAGATCAT TGAAATCAAT
    GGGGAGAGCA CAAGAGACAT GACCCATGCC AGAGCAATAG AGCTCATCAA ATCAGGCGGA
    AGGAGAGTGA GGCTACTGCT GAAGCGTGGG ACGGGACAGG TCCCAGAGTA TGACGAACCC
    GGCTCCTGGA GTGCTCCCGC TGCCGCCTCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
    GAGGACGTCC TCTCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
    CAGACAAACC CAGACCCAAC TTGGGATATC AAAAGGGAAC ACGACGTTAG GAAACCAAAG
    GAGCTTTCAG CCGGCAGCCA GAAGAAGCAG CGCCTTGGGG AGCAGAGCGA GCGCTCAGCC
    AGCCCGCAAA GGGCCGTGCA ACCAGGGCTC GAGGAGGCGC CTGGCGGCCA GGGGCAGCCT
    GAGGCCGGCA GGCCCTCCTC GGAGACCAGA GCGCCTGGGC CCGCACTGGC CGAAGCGGCG
    CGGGCTGGCA ACAAGGAGGG GCTGCAGGCC CCGGTGGGCT CCGAGCTCGG CCGGCACGGA
    GGCCCCGGCG CGGCACCTGC GTTCGTGGGC CCGGGCCGCG GCGGGGGCGC ACGGGAGGTC
    GAGGGCCGGG CCGGGGCGCG GGCGGGGTCC AGGCAGGGCT CGCGGCCCCC CGGGGGCGCC
    CCTGCGCGCA AGGCCGCCGT GGCGCCCGGG CCGTGGAAGG TGCCGGGCTC CGACAAGCTG
    CCGGGCGTCC TCAAACCCGG CGCCTCAGCC CCGGGGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324480.1
    CDS914..4795
    Translation

    Target ORF information:

    RefSeq Version XM_015468994.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468994.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCAAGGACC
    AGTTTTCGAA TGGATTCTTC TGGTCCAGAT TATAAGGAGC TGGATGTTCA CCTTCGCAGG
    ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
    ATTGGAGCCG TCATCGCCAT GGGCTCAGCT GACAGAGATG GCCGCCTACA CCCAGGAGAT
    GAGCTCGTCT ATGTGGATGG AATTCCAGTG GCTGGAAAGA CCCACCGCTA TGTCATTGAC
    CTTATGCACC ATGCAGCCCG GAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
    TGTGGAGGGG AGCCCTGCCC AGAGAATGGG AGGAGTCCAG GCTCCGTGTC GACCCACCAC
    AGTTCTCCTC GCAGTGACTA CGCAACCTAC ACCAACACCT ACAGCAACAG TAACCATGCC
    GTCCCCACCA GCAACGCCTC TCCCCCCGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGC
    GACGTGGTCA TTCACCGCAA AGAGAACGAG GGTTTCGGCT TTGTCATCAT CAGCTCCCTG
    AACAGGCCAG AGTCTGGATC CACTATAACT GTGCCACATA AAATTGGACG CATCATTGAT
    GGGAGTCCCG CAGATCGCTG TGCAAAGCTA AAAGTGGGAG ACCGGATCTT AGCGGTGAAC
    GGCCAGTCTA TCATCAACAT GCCTCATGCA GACATCGTGA AGCTCATCAA GGATGCGGGT
    CTTAGCGTCA CCCTTCGCAT CATCCCTCAG GAGGAACTCA ACAGCCCCGC TTCAGCACCC
    AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG CAGCACAGCC CCCTGGCCCA ACAGAGTCCT
    CTGGCCCAGC CCAGCCCGGC CACGCCCAAC AGTCCCATCG CCCAGCCAGC TCCGCCTCAG
    CCTCTTCAGC CTCAAGGACA TGAAAATAGT TACAGGTCAG AAGTAAAAGC GAGGCAAGAT
    GTGAAACCAG ACATCCGACA GCCTCCATTC ACGGACTACA GGCAGCCTCC ACTGGACTAC
    AGGCAACCCC CAGGAGGGGA CTACCCGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCG
    CTACTGGACT ACAGGCAGCA CTCCCCAGAC ACCAGGCAGT TCCCTCTGTC AGACTACAGG
    CAGCCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
    TTCAGCATTC GTGGAGGAAG GGAGTACAAA ATGGATTTGT ATGTGCTGAG ATTAGCAGAA
    GATGGGCCAG CAATAAGGAA TGGAAGGATG AGGGTAGGAG ATCAGATCAT TGAAATCAAT
    GGGGAGAGCA CAAGAGACAT GACCCATGCC AGAGCAATAG AGCTCATCAA ATCAGGCGGA
    AGGAGAGTGA GGCTACTGCT GAAGCGTGGG ACGGGACAGG TCCCAGAGTA TGACGAACCC
    GGCTCCTGGA GTGCTCCCGC TGCCGCCTCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
    GAGGACGTCC TCTCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
    CAGACAAACC CAGACCCAAC TTGGGATATC AAAAGGGAAC ACGACGTTAG GAAACCAAAG
    GAGCTTTCAG CCGGCAGCCA GAAGAAGCAG CGCCTTGGGG AGCAGAGCGA GCGCTCAGCC
    AGCCCGCAAA GGGCCGTGCA ACCAGGGCTC GAGGAGGCGC CTGGCGGCCA GGGGCAGCCT
    GAGGCCGGCA GGCCCTCCTC GGAGACCAGA GCGCCTGGGC CCGCACTGGC CGAAGCGGCG
    CGGGCTGGCA ACAAGGAGGG GCTGCAGGCC CCGGTGGGCT CCGAGCTCGG CCGGCACGGA
    GGCCCCGGCG CGGCACCTGC GTTCGTGGGC CCGGGCCGCG GCGGGGGCGC ACGGGAGGTC
    GAGGGCCGGG CCGGGGCGCG GGCGGGGTCC AGGCAGGGCT CGCGGCCCCC CGGGGGCGCC
    CCTGCGCGCA AGGCCGCCGT GGCGCCCGGG CCGTGGAAGG TGCCGGGCTC CGACAAGCTG
    CCGGGCGTCC TCAAACCCGG CGCCTCAGCC CCGGGGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89711
    Clone ID Related Accession (Same CDS sequence) XM_015468994.1 , XM_015463010.1
    Accession Version XM_015463010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCAAGGACC
    AGTTTTCGAA TGGATTCTTC TGGTCCAGAT TATAAGGAGC TGGATGTTCA CCTTCGCAGG
    ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
    ATTGGAGCCG TCATCGCCAT GGGCTCAGCT GACAGAGATG GCCGCCTACA CCCAGGAGAT
    GAGCTCGTCT ATGTGGATGG AATTCCAGTG GCTGGAAAGA CCCACCGCTA TGTCATTGAC
    CTTATGCACC ATGCAGCCCG GAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
    TGTGGAGGGG AGCCCTGCCC AGAGAATGGG AGGAGTCCAG GCTCCGTGTC GACCCACCAC
    AGTTCTCCTC GCAGTGACTA CGCAACCTAC ACCAACACCT ACAGCAACAG TAACCATGCC
    GTCCCCACCA GCAACGCCTC TCCCCCCGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGC
    GACGTGGTCA TTCACCGCAA AGAGAACGAG GGTTTCGGCT TTGTCATCAT CAGCTCCCTG
    AACAGGCCAG AGTCTGGATC CACTATAACT GTGCCACATA AAATTGGACG CATCATTGAT
    GGGAGTCCCG CAGATCGCTG TGCAAAGCTA AAAGTGGGAG ACCGGATCTT AGCGGTGAAC
    GGCCAGTCTA TCATCAACAT GCCTCATGCA GACATCGTGA AGCTCATCAA GGATGCGGGT
    CTTAGCGTCA CCCTTCGCAT CATCCCTCAG GAGGAACTCA ACAGCCCCGC TTCAGCACCC
    AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG CAGCACAGCC CCCTGGCCCA ACAGAGTCCT
    CTGGCCCAGC CCAGCCCGGC CACGCCCAAC AGTCCCATCG CCCAGCCAGC TCCGCCTCAG
    CCTCTTCAGC CTCAAGGACA TGAAAATAGT TACAGGTCAG AAGTAAAAGC GAGGCAAGAT
    GTGAAACCAG ACATCCGACA GCCTCCATTC ACGGACTACA GGCAGCCTCC ACTGGACTAC
    AGGCAACCCC CAGGAGGGGA CTACCCGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCG
    CTACTGGACT ACAGGCAGCA CTCCCCAGAC ACCAGGCAGT TCCCTCTGTC AGACTACAGG
    CAGCCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
    TTCAGCATTC GTGGAGGAAG GGAGTACAAA ATGGATTTGT ATGTGCTGAG ATTAGCAGAA
    GATGGGCCAG CAATAAGGAA TGGAAGGATG AGGGTAGGAG ATCAGATCAT TGAAATCAAT
    GGGGAGAGCA CAAGAGACAT GACCCATGCC AGAGCAATAG AGCTCATCAA ATCAGGCGGA
    AGGAGAGTGA GGCTACTGCT GAAGCGTGGG ACGGGACAGG TCCCAGAGTA TGACGAACCC
    GGCTCCTGGA GTGCTCCCGC TGCCGCCTCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
    GAGGACGTCC TCTCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
    CAGACAAACC CAGACCCAAC TTGGGATATC AAAAGGGAAC ACGACGTTAG GAAACCAAAG
    GAGCTTTCAG CCGGCAGCCA GAAGAAGCAG CGCCTTGGGG AGCAGAGCGA GCGCTCAGCC
    AGCCCGCAAA GGGCCGTGCA ACCAGGGCTC GAGGAGGCGC CTGGCGGCCA GGGGCAGCCT
    GAGGCCGGCA GGCCCTCCTC GGAGACCAGA GCGCCTGGGC CCGCACTGGC CGAAGCGGCG
    CGGGCTGGCA ACAAGGAGGG GCTGCAGGCC CCGGTGGGCT CCGAGCTCGG CCGGCACGGA
    GGCCCCGGCG CGGCACCTGC GTTCGTGGGC CCGGGCCGCG GCGGGGGCGC ACGGGAGGTC
    GAGGGCCGGG CCGGGGCGCG GGCGGGGTCC AGGCAGGGCT CGCGGCCCCC CGGGGGCGCC
    CCTGCGCGCA AGGCCGCCGT GGCGCCCGGG CCGTGGAAGG TGCCGGGCTC CGACAAGCTG
    CCGGGCGTCC TCAAACCCGG CGCCTCAGCC CCGGGGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318496.1
    CDS914..4795
    Translation

    Target ORF information:

    RefSeq Version XM_015463010.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCAAGGACC
    AGTTTTCGAA TGGATTCTTC TGGTCCAGAT TATAAGGAGC TGGATGTTCA CCTTCGCAGG
    ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
    ATTGGAGCCG TCATCGCCAT GGGCTCAGCT GACAGAGATG GCCGCCTACA CCCAGGAGAT
    GAGCTCGTCT ATGTGGATGG AATTCCAGTG GCTGGAAAGA CCCACCGCTA TGTCATTGAC
    CTTATGCACC ATGCAGCCCG GAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
    TGTGGAGGGG AGCCCTGCCC AGAGAATGGG AGGAGTCCAG GCTCCGTGTC GACCCACCAC
    AGTTCTCCTC GCAGTGACTA CGCAACCTAC ACCAACACCT ACAGCAACAG TAACCATGCC
    GTCCCCACCA GCAACGCCTC TCCCCCCGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGC
    GACGTGGTCA TTCACCGCAA AGAGAACGAG GGTTTCGGCT TTGTCATCAT CAGCTCCCTG
    AACAGGCCAG AGTCTGGATC CACTATAACT GTGCCACATA AAATTGGACG CATCATTGAT
    GGGAGTCCCG CAGATCGCTG TGCAAAGCTA AAAGTGGGAG ACCGGATCTT AGCGGTGAAC
    GGCCAGTCTA TCATCAACAT GCCTCATGCA GACATCGTGA AGCTCATCAA GGATGCGGGT
    CTTAGCGTCA CCCTTCGCAT CATCCCTCAG GAGGAACTCA ACAGCCCCGC TTCAGCACCC
    AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG CAGCACAGCC CCCTGGCCCA ACAGAGTCCT
    CTGGCCCAGC CCAGCCCGGC CACGCCCAAC AGTCCCATCG CCCAGCCAGC TCCGCCTCAG
    CCTCTTCAGC CTCAAGGACA TGAAAATAGT TACAGGTCAG AAGTAAAAGC GAGGCAAGAT
    GTGAAACCAG ACATCCGACA GCCTCCATTC ACGGACTACA GGCAGCCTCC ACTGGACTAC
    AGGCAACCCC CAGGAGGGGA CTACCCGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCG
    CTACTGGACT ACAGGCAGCA CTCCCCAGAC ACCAGGCAGT TCCCTCTGTC AGACTACAGG
    CAGCCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
    TTCAGCATTC GTGGAGGAAG GGAGTACAAA ATGGATTTGT ATGTGCTGAG ATTAGCAGAA
    GATGGGCCAG CAATAAGGAA TGGAAGGATG AGGGTAGGAG ATCAGATCAT TGAAATCAAT
    GGGGAGAGCA CAAGAGACAT GACCCATGCC AGAGCAATAG AGCTCATCAA ATCAGGCGGA
    AGGAGAGTGA GGCTACTGCT GAAGCGTGGG ACGGGACAGG TCCCAGAGTA TGACGAACCC
    GGCTCCTGGA GTGCTCCCGC TGCCGCCTCC CCAGGTCTGC CGGAAGTAGG CGTCTCCCTG
    GAGGACGTCC TCTCTCCATT CTCTCCATCA CATCCAGCCC CACCCTCCGA CCCTTCCCAC
    CAGACAAACC CAGACCCAAC TTGGGATATC AAAAGGGAAC ACGACGTTAG GAAACCAAAG
    GAGCTTTCAG CCGGCAGCCA GAAGAAGCAG CGCCTTGGGG AGCAGAGCGA GCGCTCAGCC
    AGCCCGCAAA GGGCCGTGCA ACCAGGGCTC GAGGAGGCGC CTGGCGGCCA GGGGCAGCCT
    GAGGCCGGCA GGCCCTCCTC GGAGACCAGA GCGCCTGGGC CCGCACTGGC CGAAGCGGCG
    CGGGCTGGCA ACAAGGAGGG GCTGCAGGCC CCGGTGGGCT CCGAGCTCGG CCGGCACGGA
    GGCCCCGGCG CGGCACCTGC GTTCGTGGGC CCGGGCCGCG GCGGGGGCGC ACGGGAGGTC
    GAGGGCCGGG CCGGGGCGCG GGCGGGGTCC AGGCAGGGCT CGCGGCCCCC CGGGGGCGCC
    CCTGCGCGCA AGGCCGCCGT GGCGCCCGGG CCGTGGAAGG TGCCGGGCTC CGACAAGCTG
    CCGGGCGTCC TCAAACCCGG CGCCTCAGCC CCGGGGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89712
    Clone ID Related Accession (Same CDS sequence) XM_015463011.1 , XM_015468995.1
    Accession Version XM_015463011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCAACAAGTG
    CCACCAAGGA CCAGTTTTCG AATGGATTCT TCTGGTCCAG ATTATAAGGA GCTGGATGTT
    CACCTTCGCA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
    CAGCCTATTT TGATTGGAGC CGTCATCGCC ATGGGCTCAG CTGACAGAGA TGGCCGCCTA
    CACCCAGGAG ATGAGCTCGT CTATGTGGAT GGAATTCCAG TGGCTGGAAA GACCCACCGC
    TATGTCATTG ACCTTATGCA CCATGCAGCC CGGAATGGGC AGGTCAACCT CACTGTGAGA
    AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAATG GGAGGAGTCC AGGCTCCGTG
    TCGACCCACC ACAGTTCTCC TCGCAGTGAC TACGCAACCT ACACCAACAC CTACAGCAAC
    AGTAACCATG CCGTCCCCAC CAGCAACGCC TCTCCCCCCG AAGGCTTCGC CTCCCACAGC
    CTGCAGACCA GCGACGTGGT CATTCACCGC AAAGAGAACG AGGGTTTCGG CTTTGTCATC
    ATCAGCTCCC TGAACAGGCC AGAGTCTGGA TCCACTATAA CTGTGCCACA TAAAATTGGA
    CGCATCATTG ATGGGAGTCC CGCAGATCGC TGTGCAAAGC TAAAAGTGGG AGACCGGATC
    TTAGCGGTGA ACGGCCAGTC TATCATCAAC ATGCCTCATG CAGACATCGT GAAGCTCATC
    AAGGATGCGG GTCTTAGCGT CACCCTTCGC ATCATCCCTC AGGAGGAACT CAACAGCCCC
    GCTTCAGCAC CCAGCTCAGA GAAGCAGAGC CCCCTGGCCC AGCAGCACAG CCCCCTGGCC
    CAACAGAGTC CTCTGGCCCA GCCCAGCCCG GCCACGCCCA ACAGTCCCAT CGCCCAGCCA
    GCTCCGCCTC AGCCTCTTCA GCCTCAAGGA CATGAAAATA GTTACAGGTC AGAAGTAAAA
    GCGAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACGGACTA CAGGCAGCCT
    CCACTGGACT ACAGGCAACC CCCAGGAGGG GACTACCCGC AGCCCCCACC CTTGGACTAC
    AGGCAGCCTC CGCTACTGGA CTACAGGCAG CACTCCCCAG ACACCAGGCA GTTCCCTCTG
    TCAGACTACA GGCAGCCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
    AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAGTACA AAATGGATTT GTATGTGCTG
    AGATTAGCAG AAGATGGGCC AGCAATAAGG AATGGAAGGA TGAGGGTAGG AGATCAGATC
    ATTGAAATCA ATGGGGAGAG CACAAGAGAC ATGACCCATG CCAGAGCAAT AGAGCTCATC
    AAATCAGGCG GAAGGAGAGT GAGGCTACTG CTGAAGCGTG GGACGGGACA GGTCCCAGAG
    TATGGAATGG TACCTTCCAG TCTCTCCATG TGCATGAAAA GTGACAAGCA TGGGTCCCCA
    TATTTCTACT TATTGGGCCA CCCTAAAGAC ACGACGAACC CGGCTCCTGG AGTGCTCCCG
    CTGCCGCCTC CCCAGGTCTG CCGGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318497.1
    CDS914..4423
    Translation

    Target ORF information:

    RefSeq Version XM_015463011.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCAACAAGTG
    CCACCAAGGA CCAGTTTTCG AATGGATTCT TCTGGTCCAG ATTATAAGGA GCTGGATGTT
    CACCTTCGCA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
    CAGCCTATTT TGATTGGAGC CGTCATCGCC ATGGGCTCAG CTGACAGAGA TGGCCGCCTA
    CACCCAGGAG ATGAGCTCGT CTATGTGGAT GGAATTCCAG TGGCTGGAAA GACCCACCGC
    TATGTCATTG ACCTTATGCA CCATGCAGCC CGGAATGGGC AGGTCAACCT CACTGTGAGA
    AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAATG GGAGGAGTCC AGGCTCCGTG
    TCGACCCACC ACAGTTCTCC TCGCAGTGAC TACGCAACCT ACACCAACAC CTACAGCAAC
    AGTAACCATG CCGTCCCCAC CAGCAACGCC TCTCCCCCCG AAGGCTTCGC CTCCCACAGC
    CTGCAGACCA GCGACGTGGT CATTCACCGC AAAGAGAACG AGGGTTTCGG CTTTGTCATC
    ATCAGCTCCC TGAACAGGCC AGAGTCTGGA TCCACTATAA CTGTGCCACA TAAAATTGGA
    CGCATCATTG ATGGGAGTCC CGCAGATCGC TGTGCAAAGC TAAAAGTGGG AGACCGGATC
    TTAGCGGTGA ACGGCCAGTC TATCATCAAC ATGCCTCATG CAGACATCGT GAAGCTCATC
    AAGGATGCGG GTCTTAGCGT CACCCTTCGC ATCATCCCTC AGGAGGAACT CAACAGCCCC
    GCTTCAGCAC CCAGCTCAGA GAAGCAGAGC CCCCTGGCCC AGCAGCACAG CCCCCTGGCC
    CAACAGAGTC CTCTGGCCCA GCCCAGCCCG GCCACGCCCA ACAGTCCCAT CGCCCAGCCA
    GCTCCGCCTC AGCCTCTTCA GCCTCAAGGA CATGAAAATA GTTACAGGTC AGAAGTAAAA
    GCGAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACGGACTA CAGGCAGCCT
    CCACTGGACT ACAGGCAACC CCCAGGAGGG GACTACCCGC AGCCCCCACC CTTGGACTAC
    AGGCAGCCTC CGCTACTGGA CTACAGGCAG CACTCCCCAG ACACCAGGCA GTTCCCTCTG
    TCAGACTACA GGCAGCCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
    AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAGTACA AAATGGATTT GTATGTGCTG
    AGATTAGCAG AAGATGGGCC AGCAATAAGG AATGGAAGGA TGAGGGTAGG AGATCAGATC
    ATTGAAATCA ATGGGGAGAG CACAAGAGAC ATGACCCATG CCAGAGCAAT AGAGCTCATC
    AAATCAGGCG GAAGGAGAGT GAGGCTACTG CTGAAGCGTG GGACGGGACA GGTCCCAGAG
    TATGGAATGG TACCTTCCAG TCTCTCCATG TGCATGAAAA GTGACAAGCA TGGGTCCCCA
    TATTTCTACT TATTGGGCCA CCCTAAAGAC ACGACGAACC CGGCTCCTGG AGTGCTCCCG
    CTGCCGCCTC CCCAGGTCTG CCGGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89712
    Clone ID Related Accession (Same CDS sequence) XM_015463011.1 , XM_015468995.1
    Accession Version XM_015468995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCAACAAGTG
    CCACCAAGGA CCAGTTTTCG AATGGATTCT TCTGGTCCAG ATTATAAGGA GCTGGATGTT
    CACCTTCGCA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
    CAGCCTATTT TGATTGGAGC CGTCATCGCC ATGGGCTCAG CTGACAGAGA TGGCCGCCTA
    CACCCAGGAG ATGAGCTCGT CTATGTGGAT GGAATTCCAG TGGCTGGAAA GACCCACCGC
    TATGTCATTG ACCTTATGCA CCATGCAGCC CGGAATGGGC AGGTCAACCT CACTGTGAGA
    AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAATG GGAGGAGTCC AGGCTCCGTG
    TCGACCCACC ACAGTTCTCC TCGCAGTGAC TACGCAACCT ACACCAACAC CTACAGCAAC
    AGTAACCATG CCGTCCCCAC CAGCAACGCC TCTCCCCCCG AAGGCTTCGC CTCCCACAGC
    CTGCAGACCA GCGACGTGGT CATTCACCGC AAAGAGAACG AGGGTTTCGG CTTTGTCATC
    ATCAGCTCCC TGAACAGGCC AGAGTCTGGA TCCACTATAA CTGTGCCACA TAAAATTGGA
    CGCATCATTG ATGGGAGTCC CGCAGATCGC TGTGCAAAGC TAAAAGTGGG AGACCGGATC
    TTAGCGGTGA ACGGCCAGTC TATCATCAAC ATGCCTCATG CAGACATCGT GAAGCTCATC
    AAGGATGCGG GTCTTAGCGT CACCCTTCGC ATCATCCCTC AGGAGGAACT CAACAGCCCC
    GCTTCAGCAC CCAGCTCAGA GAAGCAGAGC CCCCTGGCCC AGCAGCACAG CCCCCTGGCC
    CAACAGAGTC CTCTGGCCCA GCCCAGCCCG GCCACGCCCA ACAGTCCCAT CGCCCAGCCA
    GCTCCGCCTC AGCCTCTTCA GCCTCAAGGA CATGAAAATA GTTACAGGTC AGAAGTAAAA
    GCGAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACGGACTA CAGGCAGCCT
    CCACTGGACT ACAGGCAACC CCCAGGAGGG GACTACCCGC AGCCCCCACC CTTGGACTAC
    AGGCAGCCTC CGCTACTGGA CTACAGGCAG CACTCCCCAG ACACCAGGCA GTTCCCTCTG
    TCAGACTACA GGCAGCCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
    AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAGTACA AAATGGATTT GTATGTGCTG
    AGATTAGCAG AAGATGGGCC AGCAATAAGG AATGGAAGGA TGAGGGTAGG AGATCAGATC
    ATTGAAATCA ATGGGGAGAG CACAAGAGAC ATGACCCATG CCAGAGCAAT AGAGCTCATC
    AAATCAGGCG GAAGGAGAGT GAGGCTACTG CTGAAGCGTG GGACGGGACA GGTCCCAGAG
    TATGGAATGG TACCTTCCAG TCTCTCCATG TGCATGAAAA GTGACAAGCA TGGGTCCCCA
    TATTTCTACT TATTGGGCCA CCCTAAAGAC ACGACGAACC CGGCTCCTGG AGTGCTCCCG
    CTGCCGCCTC CCCAGGTCTG CCGGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324481.1
    CDS914..4423
    Translation

    Target ORF information:

    RefSeq Version XM_015468995.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGTAGA CAGCTCCTCA TCGACAGTAC CTAGGCCCAA ACCTGCCCGC
    TCCCTCGCCG CCCGGGAGCG GTCCCGCAGC GTGAGTGACC TGTCTTACTA CGGGAGCCAC
    CTCCCCGGGA GGCTCTGGCG CATCGTAGAA AAGCCACTCT TTACCCGGGA TGCCTCCCAA
    TTGAAGGGAA CGTTTCTCAG CACCACACTC AAAAAGAGCA ACATGGGGTT TGGATTTACC
    ATTATTGGCG GAGATGAGCC TGACGAGTTT CTGCAGGTGA AAAGTGTGAT TCCCGACGGG
    CCTGCAGCAC AAGACGGGAA AATGGAAACA GGTGATGTCA TTGTCTATAT TAATGAAGTT
    TGTGTCCTTG GACACACTCA TGCAGATGTA GTCAAACTTT TCCAGTCTGT TCCTATTGGT
    CAGAGTGTCA ACTTGGTGTT GTGTCGTGGC TACCCTTTGC CCTTTGATCC TGAAGATCCT
    GCTAACAGCA TGGTGCCACC CCTTGCAATA ATGGAGAGGC CACCTCCAGT GATGGTCAAT
    GGAAGACATA ACTATGAAAC ATATTTGGAA TACATTTCTC GGACCTCACA GTCAGTTCCG
    GATATAACAG ATCGGCCGCC TCATACTTTG CACTCAATGC CAGCTGATGG TCAGCTAGAT
    GGCACGTATC CACCACCTGT CCATGACGAC AATGTATCTA TGGCTTCATC TGGGGCCACC
    CAAGCTGAAC TTATGACCTT AACCATTGTG AAAGGTGCCC AGGGTTTTGG CTTCACCATT
    GCAGACAGTC CTACAGGACA GCGGGTGAAA CAAATACTTG ACATTCAGGG ATGCCCTGGC
    CTGTGTGAAG GCGACCTCAT TATTGAGATC AACCAGCAGA ATGTACAGAA CCTGAGCCAT
    ACGGAAGTAG TGGATATACT TAAGGACTGT CCCATTGGAA GCGAGACTTC TTTGATTATT
    CATCGAGGAG GTTTCTTTTC TCCATGGAAA ACCCCAAAGC CTATAATGGA TCGATGGGAG
    AACCAAGGCA GTCCTCAAAC GAGTTTATCT GCTCCGGCCA TACCACAGAA CCTGCCCTTC
    CCACCCGCCC TTCACAGGAG TTCCTTTCCT GACTCAACAG AGGCCTTTGA CCCAAGGAAG
    CCTGACCCAT ATGAGCTCTA CGAGAAATCG AGAGCCATTT ATGAAAGTAG GCAACAAGTG
    CCACCAAGGA CCAGTTTTCG AATGGATTCT TCTGGTCCAG ATTATAAGGA GCTGGATGTT
    CACCTTCGCA GGATGGAGTC TGGATTTGGC TTCAGAATCC TCGGGGGAGA TGAGCCTGGA
    CAGCCTATTT TGATTGGAGC CGTCATCGCC ATGGGCTCAG CTGACAGAGA TGGCCGCCTA
    CACCCAGGAG ATGAGCTCGT CTATGTGGAT GGAATTCCAG TGGCTGGAAA GACCCACCGC
    TATGTCATTG ACCTTATGCA CCATGCAGCC CGGAATGGGC AGGTCAACCT CACTGTGAGA
    AGAAAGGTGC TATGTGGAGG GGAGCCCTGC CCAGAGAATG GGAGGAGTCC AGGCTCCGTG
    TCGACCCACC ACAGTTCTCC TCGCAGTGAC TACGCAACCT ACACCAACAC CTACAGCAAC
    AGTAACCATG CCGTCCCCAC CAGCAACGCC TCTCCCCCCG AAGGCTTCGC CTCCCACAGC
    CTGCAGACCA GCGACGTGGT CATTCACCGC AAAGAGAACG AGGGTTTCGG CTTTGTCATC
    ATCAGCTCCC TGAACAGGCC AGAGTCTGGA TCCACTATAA CTGTGCCACA TAAAATTGGA
    CGCATCATTG ATGGGAGTCC CGCAGATCGC TGTGCAAAGC TAAAAGTGGG AGACCGGATC
    TTAGCGGTGA ACGGCCAGTC TATCATCAAC ATGCCTCATG CAGACATCGT GAAGCTCATC
    AAGGATGCGG GTCTTAGCGT CACCCTTCGC ATCATCCCTC AGGAGGAACT CAACAGCCCC
    GCTTCAGCAC CCAGCTCAGA GAAGCAGAGC CCCCTGGCCC AGCAGCACAG CCCCCTGGCC
    CAACAGAGTC CTCTGGCCCA GCCCAGCCCG GCCACGCCCA ACAGTCCCAT CGCCCAGCCA
    GCTCCGCCTC AGCCTCTTCA GCCTCAAGGA CATGAAAATA GTTACAGGTC AGAAGTAAAA
    GCGAGGCAAG ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACGGACTA CAGGCAGCCT
    CCACTGGACT ACAGGCAACC CCCAGGAGGG GACTACCCGC AGCCCCCACC CTTGGACTAC
    AGGCAGCCTC CGCTACTGGA CTACAGGCAG CACTCCCCAG ACACCAGGCA GTTCCCTCTG
    TCAGACTACA GGCAGCCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC
    AAAGGATTTG GATTCAGCAT TCGTGGAGGA AGGGAGTACA AAATGGATTT GTATGTGCTG
    AGATTAGCAG AAGATGGGCC AGCAATAAGG AATGGAAGGA TGAGGGTAGG AGATCAGATC
    ATTGAAATCA ATGGGGAGAG CACAAGAGAC ATGACCCATG CCAGAGCAAT AGAGCTCATC
    AAATCAGGCG GAAGGAGAGT GAGGCTACTG CTGAAGCGTG GGACGGGACA GGTCCCAGAG
    TATGGAATGG TACCTTCCAG TCTCTCCATG TGCATGAAAA GTGACAAGCA TGGGTCCCCA
    TATTTCTACT TATTGGGCCA CCCTAAAGAC ACGACGAACC CGGCTCCTGG AGTGCTCCCG
    CTGCCGCCTC CCCAGGTCTG CCGGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89713
    Clone ID Related Accession (Same CDS sequence) XM_015468996.1 , XM_015463012.1
    Accession Version XM_015468996.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCAAGGACC
    AGTTTTCGAA TGGATTCTTC TGGTCCAGAT TATAAGGAGC TGGATGTTCA CCTTCGCAGG
    ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
    ATTGGAGCCG TCATCGCCAT GGGCTCAGCT GACAGAGATG GCCGCCTACA CCCAGGAGAT
    GAGCTCGTCT ATGTGGATGG AATTCCAGTG GCTGGAAAGA CCCACCGCTA TGTCATTGAC
    CTTATGCACC ATGCAGCCCG GAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
    TGTGGAGGGG AGCCCTGCCC AGAGAATGGG AGGAGTCCAG GCTCCGTGTC GACCCACCAC
    AGTTCTCCTC GCAGTGACTA CGCAACCTAC ACCAACACCT ACAGCAACAG TAACCATGCC
    GTCCCCACCA GCAACGCCTC TCCCCCCGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGC
    GACGTGGTCA TTCACCGCAA AGAGAACGAG GGTTTCGGCT TTGTCATCAT CAGCTCCCTG
    AACAGGCCAG AGTCTGGATC CACTATAACT GTGCCACATA AAATTGGACG CATCATTGAT
    GGGAGTCCCG CAGATCGCTG TGCAAAGCTA AAAGTGGGAG ACCGGATCTT AGCGGTGAAC
    GGCCAGTCTA TCATCAACAT GCCTCATGCA GACATCGTGA AGCTCATCAA GGATGCGGGT
    CTTAGCGTCA CCCTTCGCAT CATCCCTCAG GAGGAACTCA ACAGCCCCGC TTCAGCACCC
    AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG CAGCACAGCC CCCTGGCCCA ACAGAGTCCT
    CTGGCCCAGC CCAGCCCGGC CACGCCCAAC AGTCCCATCG CCCAGCCAGC TCCGCCTCAG
    CCTCTTCAGC CTCAAGGACA TGAAAATAGT TACAGGTCAG AAGTAAAAGC GAGGCAAGAT
    GTGAAACCAG ACATCCGACA GCCTCCATTC ACGGACTACA GGCAGCCTCC ACTGGACTAC
    AGGCAACCCC CAGGAGGGGA CTACCCGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCG
    CTACTGGACT ACAGGCAGCA CTCCCCAGAC ACCAGGCAGT TCCCTCTGTC AGACTACAGG
    CAGCCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
    TTCAGCATTC GTGGAGGAAG GGAGTACAAA ATGGATTTGT ATGTGCTGAG ATTAGCAGAA
    GATGGGCCAG CAATAAGGAA TGGAAGGATG AGGGTAGGAG ATCAGATCAT TGAAATCAAT
    GGGGAGAGCA CAAGAGACAT GACCCATGCC AGAGCAATAG AGCTCATCAA ATCAGGCGGA
    AGGAGAGTGA GGCTACTGCT GAAGCGTGGG ACGGGACAGG TCCCAGAGTA TGGAATGGTA
    CCTTCCAGTC TCTCCATGTG CATGAAAAGT GACAAGCATG GGTCCCCATA TTTCTACTTA
    TTGGGCCACC CTAAAGACAC GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC
    CAGGTCTGCC GGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324482.1
    CDS910..4287
    Translation

    Target ORF information:

    RefSeq Version XM_015468996.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468996.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCAAGGACC
    AGTTTTCGAA TGGATTCTTC TGGTCCAGAT TATAAGGAGC TGGATGTTCA CCTTCGCAGG
    ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
    ATTGGAGCCG TCATCGCCAT GGGCTCAGCT GACAGAGATG GCCGCCTACA CCCAGGAGAT
    GAGCTCGTCT ATGTGGATGG AATTCCAGTG GCTGGAAAGA CCCACCGCTA TGTCATTGAC
    CTTATGCACC ATGCAGCCCG GAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
    TGTGGAGGGG AGCCCTGCCC AGAGAATGGG AGGAGTCCAG GCTCCGTGTC GACCCACCAC
    AGTTCTCCTC GCAGTGACTA CGCAACCTAC ACCAACACCT ACAGCAACAG TAACCATGCC
    GTCCCCACCA GCAACGCCTC TCCCCCCGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGC
    GACGTGGTCA TTCACCGCAA AGAGAACGAG GGTTTCGGCT TTGTCATCAT CAGCTCCCTG
    AACAGGCCAG AGTCTGGATC CACTATAACT GTGCCACATA AAATTGGACG CATCATTGAT
    GGGAGTCCCG CAGATCGCTG TGCAAAGCTA AAAGTGGGAG ACCGGATCTT AGCGGTGAAC
    GGCCAGTCTA TCATCAACAT GCCTCATGCA GACATCGTGA AGCTCATCAA GGATGCGGGT
    CTTAGCGTCA CCCTTCGCAT CATCCCTCAG GAGGAACTCA ACAGCCCCGC TTCAGCACCC
    AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG CAGCACAGCC CCCTGGCCCA ACAGAGTCCT
    CTGGCCCAGC CCAGCCCGGC CACGCCCAAC AGTCCCATCG CCCAGCCAGC TCCGCCTCAG
    CCTCTTCAGC CTCAAGGACA TGAAAATAGT TACAGGTCAG AAGTAAAAGC GAGGCAAGAT
    GTGAAACCAG ACATCCGACA GCCTCCATTC ACGGACTACA GGCAGCCTCC ACTGGACTAC
    AGGCAACCCC CAGGAGGGGA CTACCCGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCG
    CTACTGGACT ACAGGCAGCA CTCCCCAGAC ACCAGGCAGT TCCCTCTGTC AGACTACAGG
    CAGCCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
    TTCAGCATTC GTGGAGGAAG GGAGTACAAA ATGGATTTGT ATGTGCTGAG ATTAGCAGAA
    GATGGGCCAG CAATAAGGAA TGGAAGGATG AGGGTAGGAG ATCAGATCAT TGAAATCAAT
    GGGGAGAGCA CAAGAGACAT GACCCATGCC AGAGCAATAG AGCTCATCAA ATCAGGCGGA
    AGGAGAGTGA GGCTACTGCT GAAGCGTGGG ACGGGACAGG TCCCAGAGTA TGGAATGGTA
    CCTTCCAGTC TCTCCATGTG CATGAAAAGT GACAAGCATG GGTCCCCATA TTTCTACTTA
    TTGGGCCACC CTAAAGACAC GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC
    CAGGTCTGCC GGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89713
    Clone ID Related Accession (Same CDS sequence) XM_015468996.1 , XM_015463012.1
    Accession Version XM_015463012.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCAAGGACC
    AGTTTTCGAA TGGATTCTTC TGGTCCAGAT TATAAGGAGC TGGATGTTCA CCTTCGCAGG
    ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
    ATTGGAGCCG TCATCGCCAT GGGCTCAGCT GACAGAGATG GCCGCCTACA CCCAGGAGAT
    GAGCTCGTCT ATGTGGATGG AATTCCAGTG GCTGGAAAGA CCCACCGCTA TGTCATTGAC
    CTTATGCACC ATGCAGCCCG GAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
    TGTGGAGGGG AGCCCTGCCC AGAGAATGGG AGGAGTCCAG GCTCCGTGTC GACCCACCAC
    AGTTCTCCTC GCAGTGACTA CGCAACCTAC ACCAACACCT ACAGCAACAG TAACCATGCC
    GTCCCCACCA GCAACGCCTC TCCCCCCGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGC
    GACGTGGTCA TTCACCGCAA AGAGAACGAG GGTTTCGGCT TTGTCATCAT CAGCTCCCTG
    AACAGGCCAG AGTCTGGATC CACTATAACT GTGCCACATA AAATTGGACG CATCATTGAT
    GGGAGTCCCG CAGATCGCTG TGCAAAGCTA AAAGTGGGAG ACCGGATCTT AGCGGTGAAC
    GGCCAGTCTA TCATCAACAT GCCTCATGCA GACATCGTGA AGCTCATCAA GGATGCGGGT
    CTTAGCGTCA CCCTTCGCAT CATCCCTCAG GAGGAACTCA ACAGCCCCGC TTCAGCACCC
    AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG CAGCACAGCC CCCTGGCCCA ACAGAGTCCT
    CTGGCCCAGC CCAGCCCGGC CACGCCCAAC AGTCCCATCG CCCAGCCAGC TCCGCCTCAG
    CCTCTTCAGC CTCAAGGACA TGAAAATAGT TACAGGTCAG AAGTAAAAGC GAGGCAAGAT
    GTGAAACCAG ACATCCGACA GCCTCCATTC ACGGACTACA GGCAGCCTCC ACTGGACTAC
    AGGCAACCCC CAGGAGGGGA CTACCCGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCG
    CTACTGGACT ACAGGCAGCA CTCCCCAGAC ACCAGGCAGT TCCCTCTGTC AGACTACAGG
    CAGCCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
    TTCAGCATTC GTGGAGGAAG GGAGTACAAA ATGGATTTGT ATGTGCTGAG ATTAGCAGAA
    GATGGGCCAG CAATAAGGAA TGGAAGGATG AGGGTAGGAG ATCAGATCAT TGAAATCAAT
    GGGGAGAGCA CAAGAGACAT GACCCATGCC AGAGCAATAG AGCTCATCAA ATCAGGCGGA
    AGGAGAGTGA GGCTACTGCT GAAGCGTGGG ACGGGACAGG TCCCAGAGTA TGGAATGGTA
    CCTTCCAGTC TCTCCATGTG CATGAAAAGT GACAAGCATG GGTCCCCATA TTTCTACTTA
    TTGGGCCACC CTAAAGACAC GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC
    CAGGTCTGCC GGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318498.1
    CDS910..4287
    Translation

    Target ORF information:

    RefSeq Version XM_015463012.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC AACAAGTGCC ACCAAGGACC
    AGTTTTCGAA TGGATTCTTC TGGTCCAGAT TATAAGGAGC TGGATGTTCA CCTTCGCAGG
    ATGGAGTCTG GATTTGGCTT CAGAATCCTC GGGGGAGATG AGCCTGGACA GCCTATTTTG
    ATTGGAGCCG TCATCGCCAT GGGCTCAGCT GACAGAGATG GCCGCCTACA CCCAGGAGAT
    GAGCTCGTCT ATGTGGATGG AATTCCAGTG GCTGGAAAGA CCCACCGCTA TGTCATTGAC
    CTTATGCACC ATGCAGCCCG GAATGGGCAG GTCAACCTCA CTGTGAGAAG AAAGGTGCTA
    TGTGGAGGGG AGCCCTGCCC AGAGAATGGG AGGAGTCCAG GCTCCGTGTC GACCCACCAC
    AGTTCTCCTC GCAGTGACTA CGCAACCTAC ACCAACACCT ACAGCAACAG TAACCATGCC
    GTCCCCACCA GCAACGCCTC TCCCCCCGAA GGCTTCGCCT CCCACAGCCT GCAGACCAGC
    GACGTGGTCA TTCACCGCAA AGAGAACGAG GGTTTCGGCT TTGTCATCAT CAGCTCCCTG
    AACAGGCCAG AGTCTGGATC CACTATAACT GTGCCACATA AAATTGGACG CATCATTGAT
    GGGAGTCCCG CAGATCGCTG TGCAAAGCTA AAAGTGGGAG ACCGGATCTT AGCGGTGAAC
    GGCCAGTCTA TCATCAACAT GCCTCATGCA GACATCGTGA AGCTCATCAA GGATGCGGGT
    CTTAGCGTCA CCCTTCGCAT CATCCCTCAG GAGGAACTCA ACAGCCCCGC TTCAGCACCC
    AGCTCAGAGA AGCAGAGCCC CCTGGCCCAG CAGCACAGCC CCCTGGCCCA ACAGAGTCCT
    CTGGCCCAGC CCAGCCCGGC CACGCCCAAC AGTCCCATCG CCCAGCCAGC TCCGCCTCAG
    CCTCTTCAGC CTCAAGGACA TGAAAATAGT TACAGGTCAG AAGTAAAAGC GAGGCAAGAT
    GTGAAACCAG ACATCCGACA GCCTCCATTC ACGGACTACA GGCAGCCTCC ACTGGACTAC
    AGGCAACCCC CAGGAGGGGA CTACCCGCAG CCCCCACCCT TGGACTACAG GCAGCCTCCG
    CTACTGGACT ACAGGCAGCA CTCCCCAGAC ACCAGGCAGT TCCCTCTGTC AGACTACAGG
    CAGCCCCAGG ATTTTGATTA TTTCACTGTG GACATGGAGA AAGGAGCCAA AGGATTTGGA
    TTCAGCATTC GTGGAGGAAG GGAGTACAAA ATGGATTTGT ATGTGCTGAG ATTAGCAGAA
    GATGGGCCAG CAATAAGGAA TGGAAGGATG AGGGTAGGAG ATCAGATCAT TGAAATCAAT
    GGGGAGAGCA CAAGAGACAT GACCCATGCC AGAGCAATAG AGCTCATCAA ATCAGGCGGA
    AGGAGAGTGA GGCTACTGCT GAAGCGTGGG ACGGGACAGG TCCCAGAGTA TGGAATGGTA
    CCTTCCAGTC TCTCCATGTG CATGAAAAGT GACAAGCATG GGTCCCCATA TTTCTACTTA
    TTGGGCCACC CTAAAGACAC GACGAACCCG GCTCCTGGAG TGCTCCCGCT GCCGCCTCCC
    CAGGTCTGCC GGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89714
    Clone ID Related Accession (Same CDS sequence) XM_015468997.1 , XM_015463013.1
    Accession Version XM_015468997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC GTCCAGATTA TAAGGAGCTG
    GATGTTCACC TTCGCAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
    CCTGGACAGC CTATTTTGAT TGGAGCCGTC ATCGCCATGG GCTCAGCTGA CAGAGATGGC
    CGCCTACACC CAGGAGATGA GCTCGTCTAT GTGGATGGAA TTCCAGTGGC TGGAAAGACC
    CACCGCTATG TCATTGACCT TATGCACCAT GCAGCCCGGA ATGGGCAGGT CAACCTCACT
    GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAATGGGAG GAGTCCAGGC
    TCCGTGTCGA CCCACCACAG TTCTCCTCGC AGTGACTACG CAACCTACAC CAACACCTAC
    AGCAACAGTA ACCATGCCGT CCCCACCAGC AACGCCTCTC CCCCCGAAGG CTTCGCCTCC
    CACAGCCTGC AGACCAGCGA CGTGGTCATT CACCGCAAAG AGAACGAGGG TTTCGGCTTT
    GTCATCATCA GCTCCCTGAA CAGGCCAGAG TCTGGATCCA CTATAACTGT GCCACATAAA
    ATTGGACGCA TCATTGATGG GAGTCCCGCA GATCGCTGTG CAAAGCTAAA AGTGGGAGAC
    CGGATCTTAG CGGTGAACGG CCAGTCTATC ATCAACATGC CTCATGCAGA CATCGTGAAG
    CTCATCAAGG ATGCGGGTCT TAGCGTCACC CTTCGCATCA TCCCTCAGGA GGAACTCAAC
    AGCCCCGCTT CAGCACCCAG CTCAGAGAAG CAGAGCCCCC TGGCCCAGCA GCACAGCCCC
    CTGGCCCAAC AGAGTCCTCT GGCCCAGCCC AGCCCGGCCA CGCCCAACAG TCCCATCGCC
    CAGCCAGCTC CGCCTCAGCC TCTTCAGCCT CAAGGACATG AAAATAGTTA CAGGTCAGAA
    GTAAAAGCGA GGCAAGATGT GAAACCAGAC ATCCGACAGC CTCCATTCAC GGACTACAGG
    CAGCCTCCAC TGGACTACAG GCAACCCCCA GGAGGGGACT ACCCGCAGCC CCCACCCTTG
    GACTACAGGC AGCCTCCGCT ACTGGACTAC AGGCAGCACT CCCCAGACAC CAGGCAGTTC
    CCTCTGTCAG ACTACAGGCA GCCCCAGGAT TTTGATTATT TCACTGTGGA CATGGAGAAA
    GGAGCCAAAG GATTTGGATT CAGCATTCGT GGAGGAAGGG AGTACAAAAT GGATTTGTAT
    GTGCTGAGAT TAGCAGAAGA TGGGCCAGCA ATAAGGAATG GAAGGATGAG GGTAGGAGAT
    CAGATCATTG AAATCAATGG GGAGAGCACA AGAGACATGA CCCATGCCAG AGCAATAGAG
    CTCATCAAAT CAGGCGGAAG GAGAGTGAGG CTACTGCTGA AGCGTGGGAC GGGACAGGTC
    CCAGAGTATG GAATGGTACC TTCCAGTCTC TCCATGTGCA TGAAAAGTGA CAAGCATGGG
    TCCCCATATT TCTACTTATT GGGCCACCCT AAAGACACGA CGAACCCGGC TCCTGGAGTG
    CTCCCGCTGC CGCCTCCCCA GGTCTGCCGG AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015324483.1
    CDS909..4244
    Translation

    Target ORF information:

    RefSeq Version XM_015468997.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC GTCCAGATTA TAAGGAGCTG
    GATGTTCACC TTCGCAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
    CCTGGACAGC CTATTTTGAT TGGAGCCGTC ATCGCCATGG GCTCAGCTGA CAGAGATGGC
    CGCCTACACC CAGGAGATGA GCTCGTCTAT GTGGATGGAA TTCCAGTGGC TGGAAAGACC
    CACCGCTATG TCATTGACCT TATGCACCAT GCAGCCCGGA ATGGGCAGGT CAACCTCACT
    GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAATGGGAG GAGTCCAGGC
    TCCGTGTCGA CCCACCACAG TTCTCCTCGC AGTGACTACG CAACCTACAC CAACACCTAC
    AGCAACAGTA ACCATGCCGT CCCCACCAGC AACGCCTCTC CCCCCGAAGG CTTCGCCTCC
    CACAGCCTGC AGACCAGCGA CGTGGTCATT CACCGCAAAG AGAACGAGGG TTTCGGCTTT
    GTCATCATCA GCTCCCTGAA CAGGCCAGAG TCTGGATCCA CTATAACTGT GCCACATAAA
    ATTGGACGCA TCATTGATGG GAGTCCCGCA GATCGCTGTG CAAAGCTAAA AGTGGGAGAC
    CGGATCTTAG CGGTGAACGG CCAGTCTATC ATCAACATGC CTCATGCAGA CATCGTGAAG
    CTCATCAAGG ATGCGGGTCT TAGCGTCACC CTTCGCATCA TCCCTCAGGA GGAACTCAAC
    AGCCCCGCTT CAGCACCCAG CTCAGAGAAG CAGAGCCCCC TGGCCCAGCA GCACAGCCCC
    CTGGCCCAAC AGAGTCCTCT GGCCCAGCCC AGCCCGGCCA CGCCCAACAG TCCCATCGCC
    CAGCCAGCTC CGCCTCAGCC TCTTCAGCCT CAAGGACATG AAAATAGTTA CAGGTCAGAA
    GTAAAAGCGA GGCAAGATGT GAAACCAGAC ATCCGACAGC CTCCATTCAC GGACTACAGG
    CAGCCTCCAC TGGACTACAG GCAACCCCCA GGAGGGGACT ACCCGCAGCC CCCACCCTTG
    GACTACAGGC AGCCTCCGCT ACTGGACTAC AGGCAGCACT CCCCAGACAC CAGGCAGTTC
    CCTCTGTCAG ACTACAGGCA GCCCCAGGAT TTTGATTATT TCACTGTGGA CATGGAGAAA
    GGAGCCAAAG GATTTGGATT CAGCATTCGT GGAGGAAGGG AGTACAAAAT GGATTTGTAT
    GTGCTGAGAT TAGCAGAAGA TGGGCCAGCA ATAAGGAATG GAAGGATGAG GGTAGGAGAT
    CAGATCATTG AAATCAATGG GGAGAGCACA AGAGACATGA CCCATGCCAG AGCAATAGAG
    CTCATCAAAT CAGGCGGAAG GAGAGTGAGG CTACTGCTGA AGCGTGGGAC GGGACAGGTC
    CCAGAGTATG GAATGGTACC TTCCAGTCTC TCCATGTGCA TGAAAAGTGA CAAGCATGGG
    TCCCCATATT TCTACTTATT GGGCCACCCT AAAGACACGA CGAACCCGGC TCCTGGAGTG
    CTCCCGCTGC CGCCTCCCCA GGTCTGCCGG AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89714
    Clone ID Related Accession (Same CDS sequence) XM_015468997.1 , XM_015463013.1
    Accession Version XM_015463013.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644709.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC GTCCAGATTA TAAGGAGCTG
    GATGTTCACC TTCGCAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
    CCTGGACAGC CTATTTTGAT TGGAGCCGTC ATCGCCATGG GCTCAGCTGA CAGAGATGGC
    CGCCTACACC CAGGAGATGA GCTCGTCTAT GTGGATGGAA TTCCAGTGGC TGGAAAGACC
    CACCGCTATG TCATTGACCT TATGCACCAT GCAGCCCGGA ATGGGCAGGT CAACCTCACT
    GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAATGGGAG GAGTCCAGGC
    TCCGTGTCGA CCCACCACAG TTCTCCTCGC AGTGACTACG CAACCTACAC CAACACCTAC
    AGCAACAGTA ACCATGCCGT CCCCACCAGC AACGCCTCTC CCCCCGAAGG CTTCGCCTCC
    CACAGCCTGC AGACCAGCGA CGTGGTCATT CACCGCAAAG AGAACGAGGG TTTCGGCTTT
    GTCATCATCA GCTCCCTGAA CAGGCCAGAG TCTGGATCCA CTATAACTGT GCCACATAAA
    ATTGGACGCA TCATTGATGG GAGTCCCGCA GATCGCTGTG CAAAGCTAAA AGTGGGAGAC
    CGGATCTTAG CGGTGAACGG CCAGTCTATC ATCAACATGC CTCATGCAGA CATCGTGAAG
    CTCATCAAGG ATGCGGGTCT TAGCGTCACC CTTCGCATCA TCCCTCAGGA GGAACTCAAC
    AGCCCCGCTT CAGCACCCAG CTCAGAGAAG CAGAGCCCCC TGGCCCAGCA GCACAGCCCC
    CTGGCCCAAC AGAGTCCTCT GGCCCAGCCC AGCCCGGCCA CGCCCAACAG TCCCATCGCC
    CAGCCAGCTC CGCCTCAGCC TCTTCAGCCT CAAGGACATG AAAATAGTTA CAGGTCAGAA
    GTAAAAGCGA GGCAAGATGT GAAACCAGAC ATCCGACAGC CTCCATTCAC GGACTACAGG
    CAGCCTCCAC TGGACTACAG GCAACCCCCA GGAGGGGACT ACCCGCAGCC CCCACCCTTG
    GACTACAGGC AGCCTCCGCT ACTGGACTAC AGGCAGCACT CCCCAGACAC CAGGCAGTTC
    CCTCTGTCAG ACTACAGGCA GCCCCAGGAT TTTGATTATT TCACTGTGGA CATGGAGAAA
    GGAGCCAAAG GATTTGGATT CAGCATTCGT GGAGGAAGGG AGTACAAAAT GGATTTGTAT
    GTGCTGAGAT TAGCAGAAGA TGGGCCAGCA ATAAGGAATG GAAGGATGAG GGTAGGAGAT
    CAGATCATTG AAATCAATGG GGAGAGCACA AGAGACATGA CCCATGCCAG AGCAATAGAG
    CTCATCAAAT CAGGCGGAAG GAGAGTGAGG CTACTGCTGA AGCGTGGGAC GGGACAGGTC
    CCAGAGTATG GAATGGTACC TTCCAGTCTC TCCATGTGCA TGAAAAGTGA CAAGCATGGG
    TCCCCATATT TCTACTTATT GGGCCACCCT AAAGACACGA CGAACCCGGC TCCTGGAGTG
    CTCCCGCTGC CGCCTCCCCA GGTCTGCCGG AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318499.1
    CDS909..4244
    Translation

    Target ORF information:

    RefSeq Version XM_015463013.1
    Organism Bos taurus(cattle)
    Definition Bos taurus membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAATTGG AGAAAAGCGG TGCTCTCCTA GAAAGTGGGA CTTATGAAGA CAATTACTAC 
    GGTACCCCAA AGCCTCCAGC AGAACCAGCA CCATTATTAT TAAATGTAAC AGACCAGATA
    CTTCCTGGAG CCACTCCAAG CGCTGAAGGA AAACGAAAGA GGAATAAATC AGTGAGCAAC
    ATGGAGAAAG CAAGCATAGA GCCTCCTGAG GAGGAAGAGG AAGAGAGGCC TGTGGTCAAT
    GGAAATGGAG TAGTAGTGAC ACCAGAATCC AGTGAACCTG AAGACAAGAG TGCAGGTGCC
    TCAGGGGAGA CACCCTCCCA GCCTTATCCT GCACCAGTGT ACAGTCAGCC CGAGGAGGTG
    AAGGAGCAGA TGGATGACGC GAAACCAACT AAACCTGAGG AGAATGAGGA CTCAGACCCA
    TTGCCTGACA ACTGGGAAAT GGCTTATACA GAGAAGGGTG AAGTCTACTT CATTGACCAT
    AACACAAAGA CAACATCATG GCTGGATCCA CGACTTGCGA AAAAGGCTAA ACCTCCAGAA
    GAGTGCAAAG AAAATGAGCT TCCATATGGC TGGGAAAAAA TCGATGATCC CATATATGGC
    ACTTATTATG TCGACCACAT AAATAGAAGA ACACAGTTTG AAAACCCTGT CCTGGAAGCA
    AAAAGGAAGC TACAGCAGCA TAACATGCCC CACACAGAAC TTGGAACAAA GCCCTTGCAG
    GCCCCAGGTT TCCGAGAAAA GCCACTCTTT ACCCGGGATG CCTCCCAATT GAAGGGAACG
    TTTCTCAGCA CCACACTCAA AAAGAGCAAC ATGGGGTTTG GATTTACCAT TATTGGCGGA
    GATGAGCCTG ACGAGTTTCT GCAGGTGAAA AGTGTGATTC CCGACGGGCC TGCAGCACAA
    GACGGGAAAA TGGAAACAGG TGATGTCATT GTCTATATTA ATGAAGTTTG TGTCCTTGGA
    CACACTCATG CAGATGTAGT CAAACTTTTC CAGTCTGTTC CTATTGGTCA GAGTGTCAAC
    TTGGTGTTGT GTCGTGGCTA CCCTTTGCCC TTTGATCCTG AAGATCCTGC TAACAGCATG
    GTGCCACCCC TTGCAATAAT GGAGAGGCCA CCTCCAGTGA TGGTCAATGG AAGACATAAC
    TATGAAACAT ATTTGGAATA CATTTCTCGG ACCTCACAGT CAGTTCCGGA TATAACAGAT
    CGGCCGCCTC ATACTTTGCA CTCAATGCCA GCTGATGGTC AGCTAGATGG CACGTATCCA
    CCACCTGTCC ATGACGACAA TGTATCTATG GCTTCATCTG GGGCCACCCA AGCTGAACTT
    ATGACCTTAA CCATTGTGAA AGGTGCCCAG GGTTTTGGCT TCACCATTGC AGACAGTCCT
    ACAGGACAGC GGGTGAAACA AATACTTGAC ATTCAGGGAT GCCCTGGCCT GTGTGAAGGC
    GACCTCATTA TTGAGATCAA CCAGCAGAAT GTACAGAACC TGAGCCATAC GGAAGTAGTG
    GATATACTTA AGGACTGTCC CATTGGAAGC GAGACTTCTT TGATTATTCA TCGAGGAGGT
    TTCTTTTCTC CATGGAAAAC CCCAAAGCCT ATAATGGATC GATGGGAGAA CCAAGGCAGT
    CCTCAAACGA GTTTATCTGC TCCGGCCATA CCACAGAACC TGCCCTTCCC ACCCGCCCTT
    CACAGGAGTT CCTTTCCTGA CTCAACAGAG GCCTTTGACC CAAGGAAGCC TGACCCATAT
    GAGCTCTACG AGAAATCGAG AGCCATTTAT GAAAGTAGGC GTCCAGATTA TAAGGAGCTG
    GATGTTCACC TTCGCAGGAT GGAGTCTGGA TTTGGCTTCA GAATCCTCGG GGGAGATGAG
    CCTGGACAGC CTATTTTGAT TGGAGCCGTC ATCGCCATGG GCTCAGCTGA CAGAGATGGC
    CGCCTACACC CAGGAGATGA GCTCGTCTAT GTGGATGGAA TTCCAGTGGC TGGAAAGACC
    CACCGCTATG TCATTGACCT TATGCACCAT GCAGCCCGGA ATGGGCAGGT CAACCTCACT
    GTGAGAAGAA AGGTGCTATG TGGAGGGGAG CCCTGCCCAG AGAATGGGAG GAGTCCAGGC
    TCCGTGTCGA CCCACCACAG TTCTCCTCGC AGTGACTACG CAACCTACAC CAACACCTAC
    AGCAACAGTA ACCATGCCGT CCCCACCAGC AACGCCTCTC CCCCCGAAGG CTTCGCCTCC
    CACAGCCTGC AGACCAGCGA CGTGGTCATT CACCGCAAAG AGAACGAGGG TTTCGGCTTT
    GTCATCATCA GCTCCCTGAA CAGGCCAGAG TCTGGATCCA CTATAACTGT GCCACATAAA
    ATTGGACGCA TCATTGATGG GAGTCCCGCA GATCGCTGTG CAAAGCTAAA AGTGGGAGAC
    CGGATCTTAG CGGTGAACGG CCAGTCTATC ATCAACATGC CTCATGCAGA CATCGTGAAG
    CTCATCAAGG ATGCGGGTCT TAGCGTCACC CTTCGCATCA TCCCTCAGGA GGAACTCAAC
    AGCCCCGCTT CAGCACCCAG CTCAGAGAAG CAGAGCCCCC TGGCCCAGCA GCACAGCCCC
    CTGGCCCAAC AGAGTCCTCT GGCCCAGCCC AGCCCGGCCA CGCCCAACAG TCCCATCGCC
    CAGCCAGCTC CGCCTCAGCC TCTTCAGCCT CAAGGACATG AAAATAGTTA CAGGTCAGAA
    GTAAAAGCGA GGCAAGATGT GAAACCAGAC ATCCGACAGC CTCCATTCAC GGACTACAGG
    CAGCCTCCAC TGGACTACAG GCAACCCCCA GGAGGGGACT ACCCGCAGCC CCCACCCTTG
    GACTACAGGC AGCCTCCGCT ACTGGACTAC AGGCAGCACT CCCCAGACAC CAGGCAGTTC
    CCTCTGTCAG ACTACAGGCA GCCCCAGGAT TTTGATTATT TCACTGTGGA CATGGAGAAA
    GGAGCCAAAG GATTTGGATT CAGCATTCGT GGAGGAAGGG AGTACAAAAT GGATTTGTAT
    GTGCTGAGAT TAGCAGAAGA TGGGCCAGCA ATAAGGAATG GAAGGATGAG GGTAGGAGAT
    CAGATCATTG AAATCAATGG GGAGAGCACA AGAGACATGA CCCATGCCAG AGCAATAGAG
    CTCATCAAAT CAGGCGGAAG GAGAGTGAGG CTACTGCTGA AGCGTGGGAC GGGACAGGTC
    CCAGAGTATG GAATGGTACC TTCCAGTCTC TCCATGTGCA TGAAAAGTGA CAAGCATGGG
    TCCCCATATT TCTACTTATT GGGCCACCCT AAAGACACGA CGAACCCGGC TCCTGGAGTG
    CTCCCGCTGC CGCCTCCCCA GGTCTGCCGG AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.