NEO1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010809028.2	XP_010807330.1	neogenin isoform X2
XM_010809029.2	XP_010807331.1	neogenin isoform X3
XM_003582660.4	XP_003582708.1	neogenin isoform X6
XM_003586507.4	XP_003586555.1	neogenin isoform X3
XM_024997934.1	XP_024853702.1	neogenin isoform X5
XM_010809030.2	XP_010807332.1	neogenin isoform X7
XM_010809031.2	XP_010807333.1	neogenin isoform X9
XM_003582659.4	XP_003582707.1	neogenin isoform X4
XM_005193587.3	XP_005193644.1	neogenin isoform X5
XM_010810028.2	XP_010808330.1	neogenin isoform X7
XM_024997935.1	XP_024853703.1	neogenin isoform X6
XM_024997936.1	XP_024853704.1	neogenin isoform X7
XM_010810024.2	XP_010808326.1	neogenin isoform X2
XM_003582661.4	XP_003582709.1	neogenin isoform X8
XM_010810034.2	XP_010808336.1	neogenin isoform X9
XM_005211431.3	XP_005211488.1	neogenin isoform X1
XM_003586507.3	XP_003586555.1	neogenin isoform X6
XM_005211432.4	XP_005211489.1	neogenin isoform X2
XM_024997933.1	XP_024853701.1	neogenin isoform X4
XM_002690492.4	XP_002690538.1	neogenin isoform X4
XM_005211432.3	XP_005211489.1	neogenin isoform X5
XM_024997937.1	XP_024853705.1	neogenin isoform X8
XM_003586508.4	XP_003586556.1	neogenin isoform X8
XM_005193586.3	XP_005193643.1	neogenin isoform X1
XM_010810026.2	XP_010808328.1	neogenin isoform X3
XM_010809028.3	XP_010807330.1	neogenin isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219808	XM_024997933.1 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa219809	XM_024997934.1 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa219810	XM_024997935.1 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa219811	XM_024997936.1 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa219812	XM_024997937.1 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa06313	XM_005193586.3 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06313	XM_005211431.3 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06314	XM_003582659.4 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06314	XM_002690492.4 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06315	XM_005193587.3 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06315	XM_005211432.4 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06316	XM_003582660.4 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06316	XM_003586507.4 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06317	XM_003582661.4 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06317	XM_003586508.4 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59380	XM_010810024.2 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59380	XM_010809028.3 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59381	XM_010810026.2 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59381	XM_010809029.2 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59382	XM_010809030.2 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59382	XM_010810028.2 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59383	XM_010810034.2 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59383	XM_010809031.2 Latest version!	Bos taurus neogenin 1 (NEO1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OBa06316	XM_003586507.3	Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa06315	XM_005211432.3	Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa59380	XM_010809028.2	Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

NEO1 ( XM_024997933.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853701.1 Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa219808
Clone ID Related Accession (Same CDS sequence)	XM_024997933.1
Accession Version	XM_024997933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neogenin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCAATC TGTTCGAAAC 
ACCCCCAGCA CTGACACCAT GCCAGCCTCC TCGTCTCAAA CATGCTGTAC CGATCATCAG 
GATCCTGAAG GTGCTACCAG CTCTTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCGGGC 
CAGAGTCTCC CTACAGCCCA CGTTCGCCCT TCCCACCCAC TGAAGAGCTT TGCTGTGCCG 
GCAATCCCAC CCACCGGGCC TCCCACCTAC GATCCAGCGT TGCCAAGCAC ACCGTTACTC 
TCCCAGCAAG CTCTGAATCA TCACATCCAC GCCGTGAAGA CGGCCTCCAT CGGGACTTTA 
GGAAGGAGCC GGCCTCCTAT GCCAGTGGTG GTTCCCAGTG CCCCTGAAGT GCAGGAGACC 
ACAAGGATGC TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG 
GCCCACCTGG AAGGACTGAT GAAGGACCTA AACGCCATCA CGACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853701.1
CDS	226..4476
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_024997933.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997933.1

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCAATC TGTTCGAAAC 
ACCCCCAGCA CTGACACCAT GCCAGCCTCC TCGTCTCAAA CATGCTGTAC CGATCATCAG 
GATCCTGAAG GTGCTACCAG CTCTTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCGGGC 
CAGAGTCTCC CTACAGCCCA CGTTCGCCCT TCCCACCCAC TGAAGAGCTT TGCTGTGCCG 
GCAATCCCAC CCACCGGGCC TCCCACCTAC GATCCAGCGT TGCCAAGCAC ACCGTTACTC 
TCCCAGCAAG CTCTGAATCA TCACATCCAC GCCGTGAAGA CGGCCTCCAT CGGGACTTTA 
GGAAGGAGCC GGCCTCCTAT GCCAGTGGTG GTTCCCAGTG CCCCTGAAGT GCAGGAGACC 
ACAAGGATGC TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG 
GCCCACCTGG AAGGACTGAT GAAGGACCTA AACGCCATCA CGACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_024997934.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853702.1 Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa219809
Clone ID Related Accession (Same CDS sequence)	XM_024997934.1
Accession Version	XM_024997934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neogenin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853702.1
CDS	225..4427
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_024997934.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997934.1

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_024997935.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853703.1 Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa219810
Clone ID Related Accession (Same CDS sequence)	XM_024997935.1
Accession Version	XM_024997935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neogenin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGAATCTGT TCGAAACACC CCCAGCACTG ACACCATGCC AGCCTCCTCG 
TCTCAAACAT GCTGTACCGA TCATCAGGAT CCTGAAGGTG CTACCAGCTC TTCTTACTTG 
GCCAGCTCCC AAGAGGAAGA TTCGGGCCAG AGTCTCCCTA CAGCCCACGT TCGCCCTTCC 
CACCCACTGA AGAGCTTTGC TGTGCCGGCA ATCCCACCCA CCGGGCCTCC CACCTACGAT 
CCAGCGTTGC CAAGCACACC GTTACTCTCC CAGCAAGCTC TGAATCATCA CATCCACGCC 
GTGAAGACGG CCTCCATCGG GACTTTAGGA AGGAGCCGGC CTCCTATGCC AGTGGTGGTT 
CCCAGTGCCC CTGAAGTGCA GGAGACCACA AGGATGCTGG AAGACTCCGA GAGTAGCTAT 
GAACCAGATG AGCTGACCAA AGAGATGGCC CACCTGGAAG GACTGATGAA GGACCTAAAC 
GCCATCACGA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853703.1
CDS	227..4384
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_024997935.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997935.1

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGAATCTGT TCGAAACACC CCCAGCACTG ACACCATGCC AGCCTCCTCG 
TCTCAAACAT GCTGTACCGA TCATCAGGAT CCTGAAGGTG CTACCAGCTC TTCTTACTTG 
GCCAGCTCCC AAGAGGAAGA TTCGGGCCAG AGTCTCCCTA CAGCCCACGT TCGCCCTTCC 
CACCCACTGA AGAGCTTTGC TGTGCCGGCA ATCCCACCCA CCGGGCCTCC CACCTACGAT 
CCAGCGTTGC CAAGCACACC GTTACTCTCC CAGCAAGCTC TGAATCATCA CATCCACGCC 
GTGAAGACGG CCTCCATCGG GACTTTAGGA AGGAGCCGGC CTCCTATGCC AGTGGTGGTT 
CCCAGTGCCC CTGAAGTGCA GGAGACCACA AGGATGCTGG AAGACTCCGA GAGTAGCTAT 
GAACCAGATG AGCTGACCAA AGAGATGGCC CACCTGGAAG GACTGATGAA GGACCTAAAC 
GCCATCACGA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_024997936.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853704.1 Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.

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CloneID	OBa219811
Clone ID Related Accession (Same CDS sequence)	XM_024997936.1
Accession Version	XM_024997936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neogenin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853704.1
CDS	224..4366
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_024997936.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997936.1

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_024997937.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853705.1 Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa219812
Clone ID Related Accession (Same CDS sequence)	XM_024997937.1
Accession Version	XM_024997937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neogenin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCAATC TGTTCGAAAC 
ACCCCCAGCA CTGACACCAT GCCAGCCTCC TCGTCTCAAA CATGCTGTAC CGATCATCAG 
GATCCTGAAG GTGCTACCAG CTCTTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCGGGC 
CAGAGTCTCC CTACAGCCCA CGTTCGCCCT TCCCACCCAC TGAAGAGCTT TGCTGTGCCG 
GCAATCCCAC CCACCGGGCC TCCCACCTAC GATCCAGCGT TGCCAAGCAC ACCGTTACTC 
TCCCAGCAAG CTCTGAATCA TCACATCCAC GCCGTGAAGA CGGCCTCCAT CGGGACTTTA 
GGAAGGAGCC GGCCTCCTAT GCCAGTGGTG GTTCCCAGTG CCCCTGAAGT GCAGGAGACC 
ACAAGGATGC TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG 
GCCCACCTGG AAGGACTGAT GAAGGACCTA AACGCCATCA CGACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853705.1
CDS	226..4416
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_024997937.1
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997937.1

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCAATC TGTTCGAAAC 
ACCCCCAGCA CTGACACCAT GCCAGCCTCC TCGTCTCAAA CATGCTGTAC CGATCATCAG 
GATCCTGAAG GTGCTACCAG CTCTTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCGGGC 
CAGAGTCTCC CTACAGCCCA CGTTCGCCCT TCCCACCCAC TGAAGAGCTT TGCTGTGCCG 
GCAATCCCAC CCACCGGGCC TCCCACCTAC GATCCAGCGT TGCCAAGCAC ACCGTTACTC 
TCCCAGCAAG CTCTGAATCA TCACATCCAC GCCGTGAAGA CGGCCTCCAT CGGGACTTTA 
GGAAGGAGCC GGCCTCCTAT GCCAGTGGTG GTTCCCAGTG CCCCTGAAGT GCAGGAGACC 
ACAAGGATGC TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG 
GCCCACCTGG AAGGACTGAT GAAGGACCTA AACGCCATCA CGACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_005211431.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211488.1 Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa06313
Clone ID Related Accession (Same CDS sequence)	XM_005211431.3 , XM_005193586.3
Accession Version	XM_005211431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211431.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCC TGTGATTAGT GCCCATCCCA TCCATTCCCT CGATAACCCT 
CACCATCATT TCCACTCCAG CAGCCTCGCT TCTCCAGCTC GCAGTCATCT CTACCACCCG 
GGCAGCCCAT GGCCCATTGG CACATCCATG TCCCTTTCAG ACAGGGCCAA TTCCACAGAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211488.1
CDS	62..4504
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_005211431.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211431.3

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCC TGTGATTAGT GCCCATCCCA TCCATTCCCT CGATAACCCT 
CACCATCATT TCCACTCCAG CAGCCTCGCT TCTCCAGCTC GCAGTCATCT CTACCACCCG 
GGCAGCCCAT GGCCCATTGG CACATCCATG TCCCTTTCAG ACAGGGCCAA TTCCACAGAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_005193586.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005193643.1 Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.

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CloneID	OBa06313
Clone ID Related Accession (Same CDS sequence)	XM_005211431.3 , XM_005193586.3
Accession Version	XM_005193586.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCC TGTGATTAGT GCCCATCCCA TCCATTCCCT CGATAACCCT 
CACCATCATT TCCACTCCAG CAGCCTCGCT TCTCCAGCTC GCAGTCATCT CTACCACCCG 
GGCAGCCCAT GGCCCATTGG CACATCCATG TCCCTTTCAG ACAGGGCCAA TTCCACAGAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005193643.1
CDS	62..4504
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_005193586.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005193586.3

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCC TGTGATTAGT GCCCATCCCA TCCATTCCCT CGATAACCCT 
CACCATCATT TCCACTCCAG CAGCCTCGCT TCTCCAGCTC GCAGTCATCT CTACCACCCG 
GGCAGCCCAT GGCCCATTGG CACATCCATG TCCCTTTCAG ACAGGGCCAA TTCCACAGAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_003582659.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582707.1 Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.

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CloneID	OBa06314
Clone ID Related Accession (Same CDS sequence)	XM_003582659.4 , XM_002690492.4
Accession Version	XM_003582659.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCC TGTGATTAGT GCCCATCCCA TCCATTCCCT CGATAACCCT 
CACCATCATT TCCACTCCAG CAGCCTCGCT TCTCCAGCTC GCAGTCATCT CTACCACCCG 
GGCAGCCCAT GGCCCATTGG CACATCCATG TCCCTTTCAG ACAGGGCCAA TTCCACAGAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582707.1
CDS	1..4383
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_003582659.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582659.4

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCC TGTGATTAGT GCCCATCCCA TCCATTCCCT CGATAACCCT 
CACCATCATT TCCACTCCAG CAGCCTCGCT TCTCCAGCTC GCAGTCATCT CTACCACCCG 
GGCAGCCCAT GGCCCATTGG CACATCCATG TCCCTTTCAG ACAGGGCCAA TTCCACAGAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_002690492.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002690538.1 Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa06314
Clone ID Related Accession (Same CDS sequence)	XM_003582659.4 , XM_002690492.4
Accession Version	XM_002690492.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690492.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCC TGTGATTAGT GCCCATCCCA TCCATTCCCT CGATAACCCT 
CACCATCATT TCCACTCCAG CAGCCTCGCT TCTCCAGCTC GCAGTCATCT CTACCACCCG 
GGCAGCCCAT GGCCCATTGG CACATCCATG TCCCTTTCAG ACAGGGCCAA TTCCACAGAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002690538.1
CDS	1..4383
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_002690492.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690492.4

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCC TGTGATTAGT GCCCATCCCA TCCATTCCCT CGATAACCCT 
CACCATCATT TCCACTCCAG CAGCCTCGCT TCTCCAGCTC GCAGTCATCT CTACCACCCG 
GGCAGCCCAT GGCCCATTGG CACATCCATG TCCCTTTCAG ACAGGGCCAA TTCCACAGAA 
TCTGTTCGAA ACACCCCCAG CACTGACACC ATGCCAGCCT CCTCGTCTCA AACATGCTGT 
ACCGATCATC AGGATCCTGA AGGTGCTACC AGCTCTTCTT ACTTGGCCAG CTCCCAAGAG 
GAAGATTCGG GCCAGAGTCT CCCTACAGCC CACGTTCGCC CTTCCCACCC ACTGAAGAGC 
TTTGCTGTGC CGGCAATCCC ACCCACCGGG CCTCCCACCT ACGATCCAGC GTTGCCAAGC 
ACACCGTTAC TCTCCCAGCA AGCTCTGAAT CATCACATCC ACGCCGTGAA GACGGCCTCC 
ATCGGGACTT TAGGAAGGAG CCGGCCTCCT ATGCCAGTGG TGGTTCCCAG TGCCCCTGAA 
GTGCAGGAGA CCACAAGGAT GCTGGAAGAC TCCGAGAGTA GCTATGAACC AGATGAGCTG 
ACCAAAGAGA TGGCCCACCT GGAAGGACTG ATGAAGGACC TAAACGCCAT CACGACAGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_005193587.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005193644.1 Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa06315
Clone ID Related Accession (Same CDS sequence)	XM_005193587.3 , XM_005211432.4 , XM_005211432.3
Accession Version	XM_005193587.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCCT 
GTGATTAGTG CCCATCCCAT CCATTCCCTC GATAACCCTC ACCATCATTT CCACTCCAGC 
AGCCTCGCTT CTCCAGCTCG CAGTCATCTC TACCACCCGG GCAGCCCATG GCCCATTGGC 
ACATCCATGT CCCTTTCAGA CAGGGCCAAT TCCACAGAAT CTGTTCGAAA CACCCCCAGC 
ACTGACACCA TGCCAGCCTC CTCGTCTCAA ACATGCTGTA CCGATCATCA GGATCCTGAA 
GGTGCTACCA GCTCTTCTTA CTTGGCCAGC TCCCAAGAGG AAGATTCGGG CCAGAGTCTC 
CCTACAGCCC ACGTTCGCCC TTCCCACCCA CTGAAGAGCT TTGCTGTGCC GGCAATCCCA 
CCCACCGGGC CTCCCACCTA CGATCCAGCG TTGCCAAGCA CACCGTTACT CTCCCAGCAA 
GCTCTGAATC ATCACATCCA CGCCGTGAAG ACGGCCTCCA TCGGGACTTT AGGAAGGAGC 
CGGCCTCCTA TGCCAGTGGT GGTTCCCAGT GCCCCTGAAG TGCAGGAGAC CACAAGGATG 
CTGGAAGACT CCGAGAGTAG CTATGAACCA GATGAGCTGA CCAAAGAGAT GGCCCACCTG 
GAAGGACTGA TGAAGGACCT AAACGCCATC ACGACAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005193644.1
CDS	60..4421
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_005193587.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005193587.3

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCCT 
GTGATTAGTG CCCATCCCAT CCATTCCCTC GATAACCCTC ACCATCATTT CCACTCCAGC 
AGCCTCGCTT CTCCAGCTCG CAGTCATCTC TACCACCCGG GCAGCCCATG GCCCATTGGC 
ACATCCATGT CCCTTTCAGA CAGGGCCAAT TCCACAGAAT CTGTTCGAAA CACCCCCAGC 
ACTGACACCA TGCCAGCCTC CTCGTCTCAA ACATGCTGTA CCGATCATCA GGATCCTGAA 
GGTGCTACCA GCTCTTCTTA CTTGGCCAGC TCCCAAGAGG AAGATTCGGG CCAGAGTCTC 
CCTACAGCCC ACGTTCGCCC TTCCCACCCA CTGAAGAGCT TTGCTGTGCC GGCAATCCCA 
CCCACCGGGC CTCCCACCTA CGATCCAGCG TTGCCAAGCA CACCGTTACT CTCCCAGCAA 
GCTCTGAATC ATCACATCCA CGCCGTGAAG ACGGCCTCCA TCGGGACTTT AGGAAGGAGC 
CGGCCTCCTA TGCCAGTGGT GGTTCCCAGT GCCCCTGAAG TGCAGGAGAC CACAAGGATG 
CTGGAAGACT CCGAGAGTAG CTATGAACCA GATGAGCTGA CCAAAGAGAT GGCCCACCTG 
GAAGGACTGA TGAAGGACCT AAACGCCATC ACGACAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_005211432.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211489.1 Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.

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CloneID	OBa06315
Clone ID Related Accession (Same CDS sequence)	XM_005193587.3 , XM_005211432.4 , XM_005211432.3
Accession Version	XM_005211432.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211432.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCCT 
GTGATTAGTG CCCATCCCAT CCATTCCCTC GATAACCCTC ACCATCATTT CCACTCCAGC 
AGCCTCGCTT CTCCAGCTCG CAGTCATCTC TACCACCCGG GCAGCCCATG GCCCATTGGC 
ACATCCATGT CCCTTTCAGA CAGGGCCAAT TCCACAGAAT CTGTTCGAAA CACCCCCAGC 
ACTGACACCA TGCCAGCCTC CTCGTCTCAA ACATGCTGTA CCGATCATCA GGATCCTGAA 
GGTGCTACCA GCTCTTCTTA CTTGGCCAGC TCCCAAGAGG AAGATTCGGG CCAGAGTCTC 
CCTACAGCCC ACGTTCGCCC TTCCCACCCA CTGAAGAGCT TTGCTGTGCC GGCAATCCCA 
CCCACCGGGC CTCCCACCTA CGATCCAGCG TTGCCAAGCA CACCGTTACT CTCCCAGCAA 
GCTCTGAATC ATCACATCCA CGCCGTGAAG ACGGCCTCCA TCGGGACTTT AGGAAGGAGC 
CGGCCTCCTA TGCCAGTGGT GGTTCCCAGT GCCCCTGAAG TGCAGGAGAC CACAAGGATG 
CTGGAAGACT CCGAGAGTAG CTATGAACCA GATGAGCTGA CCAAAGAGAT GGCCCACCTG 
GAAGGACTGA TGAAGGACCT AAACGCCATC ACGACAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211489.1
CDS	224..4585
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_005211432.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211432.4

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCCT 
GTGATTAGTG CCCATCCCAT CCATTCCCTC GATAACCCTC ACCATCATTT CCACTCCAGC 
AGCCTCGCTT CTCCAGCTCG CAGTCATCTC TACCACCCGG GCAGCCCATG GCCCATTGGC 
ACATCCATGT CCCTTTCAGA CAGGGCCAAT TCCACAGAAT CTGTTCGAAA CACCCCCAGC 
ACTGACACCA TGCCAGCCTC CTCGTCTCAA ACATGCTGTA CCGATCATCA GGATCCTGAA 
GGTGCTACCA GCTCTTCTTA CTTGGCCAGC TCCCAAGAGG AAGATTCGGG CCAGAGTCTC 
CCTACAGCCC ACGTTCGCCC TTCCCACCCA CTGAAGAGCT TTGCTGTGCC GGCAATCCCA 
CCCACCGGGC CTCCCACCTA CGATCCAGCG TTGCCAAGCA CACCGTTACT CTCCCAGCAA 
GCTCTGAATC ATCACATCCA CGCCGTGAAG ACGGCCTCCA TCGGGACTTT AGGAAGGAGC 
CGGCCTCCTA TGCCAGTGGT GGTTCCCAGT GCCCCTGAAG TGCAGGAGAC CACAAGGATG 
CTGGAAGACT CCGAGAGTAG CTATGAACCA GATGAGCTGA CCAAAGAGAT GGCCCACCTG 
GAAGGACTGA TGAAGGACCT AAACGCCATC ACGACAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_005211432.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211489.1 Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.

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CloneID	OBa06315
Clone ID Related Accession (Same CDS sequence)	XM_005193587.3 , XM_005211432.4 , XM_005211432.3
Accession Version	XM_005211432.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211432.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCCT 
GTGATTAGTG CCCATCCCAT CCATTCCCTC GATAACCCTC ACCATCATTT CCACTCCAGC 
AGCCTCGCTT CTCCAGCTCG CAGTCATCTC TACCACCCGG GCAGCCCATG GCCCATTGGC 
ACATCCATGT CCCTTTCAGA CAGGGCCAAT TCCACAGAAT CTGTTCGAAA CACCCCCAGC 
ACTGACACCA TGCCAGCCTC CTCGTCTCAA ACATGCTGTA CCGATCATCA GGATCCTGAA 
GGTGCTACCA GCTCTTCTTA CTTGGCCAGC TCCCAAGAGG AAGATTCGGG CCAGAGTCTC 
CCTACAGCCC ACGTTCGCCC TTCCCACCCA CTGAAGAGCT TTGCTGTGCC GGCAATCCCA 
CCCACCGGGC CTCCCACCTA CGATCCAGCG TTGCCAAGCA CACCGTTACT CTCCCAGCAA 
GCTCTGAATC ATCACATCCA CGCCGTGAAG ACGGCCTCCA TCGGGACTTT AGGAAGGAGC 
CGGCCTCCTA TGCCAGTGGT GGTTCCCAGT GCCCCTGAAG TGCAGGAGAC CACAAGGATG 
CTGGAAGACT CCGAGAGTAG CTATGAACCA GATGAGCTGA CCAAAGAGAT GGCCCACCTG 
GAAGGACTGA TGAAGGACCT AAACGCCATC ACGACAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211489.1
CDS	60..4421
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_005211432.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211432.3

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC CTCGGGGTCT 
GCAGGGAAAG GAAGCCGACT GCCAGACCTG GGATCCGACT ACAAACCTCC AATGAGCGGC 
AGTAACAGCC CTCATGGAAG CCCCACCTCT CCTCTGGACA GTAACATGCT GCTGGTCATC 
ATCGTTTCCG TTGGTGTCAT CACCATCGTG GTGGTCGTGA TCATTGCTGT TTTCTGCACC 
CGGCGGACTA CCTCTCACCA GAAAAAGAAA CGAGCTGCTT GCAAATCAGT GAATGGCTCC 
CACAAGTATA AAGGGAACTC CAAAGATGTC AAACCCCCAG ACCTCTGGAT CCATCACGAG 
AGACTGGAGC TCAAACCCAT TGATAAGTCT CCAGACCCAA ACCCCATCAT GACTGATACA 
CCAATTCCTC GTAATTCTCA AGATATCACA CCAGTTGACA ATTCCATGGA CAGCAACATC 
CATCAAAGGC GGAATTCATA TAGAGGGCAT GAATCAGAGG ACAGCATGTC TACACTGGCA 
GGAAGGCGGG GAATGAGACC AAAAATGATG ATGCCCTTTG ACTCCCAGCC ACCCCAGCCT 
GTGATTAGTG CCCATCCCAT CCATTCCCTC GATAACCCTC ACCATCATTT CCACTCCAGC 
AGCCTCGCTT CTCCAGCTCG CAGTCATCTC TACCACCCGG GCAGCCCATG GCCCATTGGC 
ACATCCATGT CCCTTTCAGA CAGGGCCAAT TCCACAGAAT CTGTTCGAAA CACCCCCAGC 
ACTGACACCA TGCCAGCCTC CTCGTCTCAA ACATGCTGTA CCGATCATCA GGATCCTGAA 
GGTGCTACCA GCTCTTCTTA CTTGGCCAGC TCCCAAGAGG AAGATTCGGG CCAGAGTCTC 
CCTACAGCCC ACGTTCGCCC TTCCCACCCA CTGAAGAGCT TTGCTGTGCC GGCAATCCCA 
CCCACCGGGC CTCCCACCTA CGATCCAGCG TTGCCAAGCA CACCGTTACT CTCCCAGCAA 
GCTCTGAATC ATCACATCCA CGCCGTGAAG ACGGCCTCCA TCGGGACTTT AGGAAGGAGC 
CGGCCTCCTA TGCCAGTGGT GGTTCCCAGT GCCCCTGAAG TGCAGGAGAC CACAAGGATG 
CTGGAAGACT CCGAGAGTAG CTATGAACCA GATGAGCTGA CCAAAGAGAT GGCCCACCTG 
GAAGGACTGA TGAAGGACCT AAACGCCATC ACGACAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_003582660.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582708.1 Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa06316
Clone ID Related Accession (Same CDS sequence)	XM_003582660.4 , XM_003586507.4 , XM_003586507.3
Accession Version	XM_003582660.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582660.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582708.1
CDS	1..4350
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_003582660.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582660.4

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_003586507.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586555.1 Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa06316
Clone ID Related Accession (Same CDS sequence)	XM_003582660.4 , XM_003586507.4 , XM_003586507.3
Accession Version	XM_003586507.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neogenin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586507.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586555.1
CDS	225..4574
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_003586507.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586507.4

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_003586507.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586555.1 Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.

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CloneID	OBa06316
Clone ID Related Accession (Same CDS sequence)	XM_003582660.4 , XM_003586507.4 , XM_003586507.3
Accession Version	XM_003586507.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586555.1
CDS	1..4350
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_003586507.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586507.3

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_003582661.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582709.1 Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.

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CloneID	OBa06317
Clone ID Related Accession (Same CDS sequence)	XM_003582661.4 , XM_003586508.4
Accession Version	XM_003582661.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582661.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCA ATCTGTTCGA AACACCCCCA GCACTGACAC CATGCCAGCC 
TCCTCGTCTC AAACATGCTG TACCGATCAT CAGGATCCTG AAGGTGCTAC CAGCTCTTCT 
TACTTGGCCA GCTCCCAAGA GGAAGATTCG GGCCAGAGTC TCCCTACAGC CCACGTTCGC 
CCTTCCCACC CACTGAAGAG CTTTGCTGTG CCGGCAATCC CACCCACCGG GCCTCCCACC 
TACGATCCAG CGTTGCCAAG CACACCGTTA CTCTCCCAGC AAGCTCTGAA TCATCACATC 
CACGCCGTGA AGACGGCCTC CATCGGGACT TTAGGAAGGA GCCGGCCTCC TATGCCAGTG 
GTGGTTCCCA GTGCCCCTGA AGTGCAGGAG ACCACAAGGA TGCTGGAAGA CTCCGAGAGT 
AGCTATGAAC CAGATGAGCT GACCAAAGAG ATGGCCCACC TGGAAGGACT GATGAAGGAC 
CTAAACGCCA TCACGACAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003582709.1
CDS	1..4224
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_003582661.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003582661.4

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCA ATCTGTTCGA AACACCCCCA GCACTGACAC CATGCCAGCC 
TCCTCGTCTC AAACATGCTG TACCGATCAT CAGGATCCTG AAGGTGCTAC CAGCTCTTCT 
TACTTGGCCA GCTCCCAAGA GGAAGATTCG GGCCAGAGTC TCCCTACAGC CCACGTTCGC 
CCTTCCCACC CACTGAAGAG CTTTGCTGTG CCGGCAATCC CACCCACCGG GCCTCCCACC 
TACGATCCAG CGTTGCCAAG CACACCGTTA CTCTCCCAGC AAGCTCTGAA TCATCACATC 
CACGCCGTGA AGACGGCCTC CATCGGGACT TTAGGAAGGA GCCGGCCTCC TATGCCAGTG 
GTGGTTCCCA GTGCCCCTGA AGTGCAGGAG ACCACAAGGA TGCTGGAAGA CTCCGAGAGT 
AGCTATGAAC CAGATGAGCT GACCAAAGAG ATGGCCCACC TGGAAGGACT GATGAAGGAC 
CTAAACGCCA TCACGACAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_003586508.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586556.1 Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa06317
Clone ID Related Accession (Same CDS sequence)	XM_003582661.4 , XM_003586508.4
Accession Version	XM_003586508.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586508.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCA ATCTGTTCGA AACACCCCCA GCACTGACAC CATGCCAGCC 
TCCTCGTCTC AAACATGCTG TACCGATCAT CAGGATCCTG AAGGTGCTAC CAGCTCTTCT 
TACTTGGCCA GCTCCCAAGA GGAAGATTCG GGCCAGAGTC TCCCTACAGC CCACGTTCGC 
CCTTCCCACC CACTGAAGAG CTTTGCTGTG CCGGCAATCC CACCCACCGG GCCTCCCACC 
TACGATCCAG CGTTGCCAAG CACACCGTTA CTCTCCCAGC AAGCTCTGAA TCATCACATC 
CACGCCGTGA AGACGGCCTC CATCGGGACT TTAGGAAGGA GCCGGCCTCC TATGCCAGTG 
GTGGTTCCCA GTGCCCCTGA AGTGCAGGAG ACCACAAGGA TGCTGGAAGA CTCCGAGAGT 
AGCTATGAAC CAGATGAGCT GACCAAAGAG ATGGCCCACC TGGAAGGACT GATGAAGGAC 
CTAAACGCCA TCACGACAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586556.1
CDS	1..4224
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_003586508.4
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586508.4

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCA ATCTGTTCGA AACACCCCCA GCACTGACAC CATGCCAGCC 
TCCTCGTCTC AAACATGCTG TACCGATCAT CAGGATCCTG AAGGTGCTAC CAGCTCTTCT 
TACTTGGCCA GCTCCCAAGA GGAAGATTCG GGCCAGAGTC TCCCTACAGC CCACGTTCGC 
CCTTCCCACC CACTGAAGAG CTTTGCTGTG CCGGCAATCC CACCCACCGG GCCTCCCACC 
TACGATCCAG CGTTGCCAAG CACACCGTTA CTCTCCCAGC AAGCTCTGAA TCATCACATC 
CACGCCGTGA AGACGGCCTC CATCGGGACT TTAGGAAGGA GCCGGCCTCC TATGCCAGTG 
GTGGTTCCCA GTGCCCCTGA AGTGCAGGAG ACCACAAGGA TGCTGGAAGA CTCCGAGAGT 
AGCTATGAAC CAGATGAGCT GACCAAAGAG ATGGCCCACC TGGAAGGACT GATGAAGGAC 
CTAAACGCCA TCACGACAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010809028.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807330.1 Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa59380
Clone ID Related Accession (Same CDS sequence)	XM_010809028.2 , XM_010810024.2 , XM_010809028.3
Accession Version	XM_010809028.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807330.1
CDS	61..4470
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010809028.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809028.2

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010810024.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808326.1 Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.

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CloneID	OBa59380
Clone ID Related Accession (Same CDS sequence)	XM_010809028.2 , XM_010810024.2 , XM_010809028.3
Accession Version	XM_010810024.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808326.1
CDS	61..4470
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010810024.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810024.2

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010809028.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807330.1 Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.

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CloneID	OBa59380
Clone ID Related Accession (Same CDS sequence)	XM_010809028.2 , XM_010810024.2 , XM_010809028.3
Accession Version	XM_010809028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	neogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807330.1
CDS	225..4634
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010809028.3
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809028.3

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCCT CGGGGTCTGC AGGGAAAGGA 
AGCCGACTGC CAGACCTGGG ATCCGACTAC AAACCTCCAA TGAGCGGCAG TAACAGCCCT 
CATGGAAGCC CCACCTCTCC TCTGGACAGT AACATGCTGC TGGTCATCAT CGTTTCCGTT 
GGTGTCATCA CCATCGTGGT GGTCGTGATC ATTGCTGTTT TCTGCACCCG GCGGACTACC 
TCTCACCAGA AAAAGAAACG AGCTGCTTGC AAATCAGTGA ATGGCTCCCA CAAGTATAAA 
GGGAACTCCA AAGATGTCAA ACCCCCAGAC CTCTGGATCC ATCACGAGAG ACTGGAGCTC 
AAACCCATTG ATAAGTCTCC AGACCCAAAC CCCATCATGA CTGATACACC AATTCCTCGT 
AATTCTCAAG ATATCACACC AGTTGACAAT TCCATGGACA GCAACATCCA TCAAAGGCGG 
AATTCATATA GAGGGCATGA ATCAGAGGAC AGCATGTCTA CACTGGCAGG AAGGCGGGGA 
ATGAGACCAA AAATGATGAT GCCCTTTGAC TCCCAGCCAC CCCAGCCTGT GATTAGTGCC 
CATCCCATCC ATTCCCTCGA TAACCCTCAC CATCATTTCC ACTCCAGCAG CCTCGCTTCT 
CCAGCTCGCA GTCATCTCTA CCACCCGGGC AGCCCATGGC CCATTGGCAC ATCCATGTCC 
CTTTCAGACA GGGCCAATTC CACAGAATCT GTTCGAAACA CCCCCAGCAC TGACACCATG 
CCAGCCTCCT CGTCTCAAAC ATGCTGTACC GATCATCAGG ATCCTGAAGG TGCTACCAGC 
TCTTCTTACT TGGCCAGCTC CCAAGAGGAA GATTCGGGCC AGAGTCTCCC TACAGCCCAC 
GTTCGCCCTT CCCACCCACT GAAGAGCTTT GCTGTGCCGG CAATCCCACC CACCGGGCCT 
CCCACCTACG ATCCAGCGTT GCCAAGCACA CCGTTACTCT CCCAGCAAGC TCTGAATCAT 
CACATCCACG CCGTGAAGAC GGCCTCCATC GGGACTTTAG GAAGGAGCCG GCCTCCTATG 
CCAGTGGTGG TTCCCAGTGC CCCTGAAGTG CAGGAGACCA CAAGGATGCT GGAAGACTCC 
GAGAGTAGCT ATGAACCAGA TGAGCTGACC AAAGAGATGG CCCACCTGGA AGGACTGATG 
AAGGACCTAA ACGCCATCAC GACAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010809029.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807331.1 Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa59381
Clone ID Related Accession (Same CDS sequence)	XM_010809029.2 , XM_010810026.2
Accession Version	XM_010809029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC GGACTCCTCT 
GATAAAATGG CTAATGATCA AGCCTCGGGG TCTGCAGGGA AAGGAAGCCG ACTGCCAGAC 
CTGGGATCCG ACTACAAACC TCCAATGAGC GGCAGTAACA GCCCTCATGG AAGCCCCACC 
TCTCCTCTGG ACAGTAACAT GCTGCTGGTC ATCATCGTTT CCGTTGGTGT CATCACCATC 
GTGGTGGTCG TGATCATTGC TGTTTTCTGC ACCCGGCGGA CTACCTCTCA CCAGAAAAAG 
AAACGAGCTG CTTGCAAATC AGTGAATGGC TCCCACAAGT ATAAAGGGAA CTCCAAAGAT 
GTCAAACCCC CAGACCTCTG GATCCATCAC GAGAGACTGG AGCTCAAACC CATTGATAAG 
TCTCCAGACC CAAACCCCAT CATGACTGAT ACACCAATTC CTCGTAATTC TCAAGATATC 
ACACCAGTTG ACAATTCCAT GGACAGCAAC ATCCATCAAA GGCGGAATTC ATATAGAGGG 
CATGAATCAG AGGACAGCAT GTCTACACTG GCAGGAAGGC GGGGAATGAG ACCAAAAATG 
ATGATGCCCT TTGACTCCCA GCCACCCCAG CCTGTGATTA GTGCCCATCC CATCCATTCC 
CTCGATAACC CTCACCATCA TTTCCACTCC AGCAGCCTCG CTTCTCCAGC TCGCAGTCAT 
CTCTACCACC CGGGCAGCCC ATGGCCCATT GGCACATCCA TGTCCCTTTC AGACAGGGCC 
AATTCCACAG AATCTGTTCG AAACACCCCC AGCACTGACA CCATGCCAGC CTCCTCGTCT 
CAAACATGCT GTACCGATCA TCAGGATCCT GAAGGTGCTA CCAGCTCTTC TTACTTGGCC 
AGCTCCCAAG AGGAAGATTC GGGCCAGAGT CTCCCTACAG CCCACGTTCG CCCTTCCCAC 
CCACTGAAGA GCTTTGCTGT GCCGGCAATC CCACCCACCG GGCCTCCCAC CTACGATCCA 
GCGTTGCCAA GCACACCGTT ACTCTCCCAG CAAGCTCTGA ATCATCACAT CCACGCCGTG 
AAGACGGCCT CCATCGGGAC TTTAGGAAGG AGCCGGCCTC CTATGCCAGT GGTGGTTCCC 
AGTGCCCCTG AAGTGCAGGA GACCACAAGG ATGCTGGAAG ACTCCGAGAG TAGCTATGAA 
CCAGATGAGC TGACCAAAGA GATGGCCCAC CTGGAAGGAC TGATGAAGGA CCTAAACGCC 
ATCACGACAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807331.1
CDS	61..4455
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010809029.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809029.2

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC GGACTCCTCT 
GATAAAATGG CTAATGATCA AGCCTCGGGG TCTGCAGGGA AAGGAAGCCG ACTGCCAGAC 
CTGGGATCCG ACTACAAACC TCCAATGAGC GGCAGTAACA GCCCTCATGG AAGCCCCACC 
TCTCCTCTGG ACAGTAACAT GCTGCTGGTC ATCATCGTTT CCGTTGGTGT CATCACCATC 
GTGGTGGTCG TGATCATTGC TGTTTTCTGC ACCCGGCGGA CTACCTCTCA CCAGAAAAAG 
AAACGAGCTG CTTGCAAATC AGTGAATGGC TCCCACAAGT ATAAAGGGAA CTCCAAAGAT 
GTCAAACCCC CAGACCTCTG GATCCATCAC GAGAGACTGG AGCTCAAACC CATTGATAAG 
TCTCCAGACC CAAACCCCAT CATGACTGAT ACACCAATTC CTCGTAATTC TCAAGATATC 
ACACCAGTTG ACAATTCCAT GGACAGCAAC ATCCATCAAA GGCGGAATTC ATATAGAGGG 
CATGAATCAG AGGACAGCAT GTCTACACTG GCAGGAAGGC GGGGAATGAG ACCAAAAATG 
ATGATGCCCT TTGACTCCCA GCCACCCCAG CCTGTGATTA GTGCCCATCC CATCCATTCC 
CTCGATAACC CTCACCATCA TTTCCACTCC AGCAGCCTCG CTTCTCCAGC TCGCAGTCAT 
CTCTACCACC CGGGCAGCCC ATGGCCCATT GGCACATCCA TGTCCCTTTC AGACAGGGCC 
AATTCCACAG AATCTGTTCG AAACACCCCC AGCACTGACA CCATGCCAGC CTCCTCGTCT 
CAAACATGCT GTACCGATCA TCAGGATCCT GAAGGTGCTA CCAGCTCTTC TTACTTGGCC 
AGCTCCCAAG AGGAAGATTC GGGCCAGAGT CTCCCTACAG CCCACGTTCG CCCTTCCCAC 
CCACTGAAGA GCTTTGCTGT GCCGGCAATC CCACCCACCG GGCCTCCCAC CTACGATCCA 
GCGTTGCCAA GCACACCGTT ACTCTCCCAG CAAGCTCTGA ATCATCACAT CCACGCCGTG 
AAGACGGCCT CCATCGGGAC TTTAGGAAGG AGCCGGCCTC CTATGCCAGT GGTGGTTCCC 
AGTGCCCCTG AAGTGCAGGA GACCACAAGG ATGCTGGAAG ACTCCGAGAG TAGCTATGAA 
CCAGATGAGC TGACCAAAGA GATGGCCCAC CTGGAAGGAC TGATGAAGGA CCTAAACGCC 
ATCACGACAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010810026.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808328.1 Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa59381
Clone ID Related Accession (Same CDS sequence)	XM_010809029.2 , XM_010810026.2
Accession Version	XM_010810026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810026.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC GGACTCCTCT 
GATAAAATGG CTAATGATCA AGCCTCGGGG TCTGCAGGGA AAGGAAGCCG ACTGCCAGAC 
CTGGGATCCG ACTACAAACC TCCAATGAGC GGCAGTAACA GCCCTCATGG AAGCCCCACC 
TCTCCTCTGG ACAGTAACAT GCTGCTGGTC ATCATCGTTT CCGTTGGTGT CATCACCATC 
GTGGTGGTCG TGATCATTGC TGTTTTCTGC ACCCGGCGGA CTACCTCTCA CCAGAAAAAG 
AAACGAGCTG CTTGCAAATC AGTGAATGGC TCCCACAAGT ATAAAGGGAA CTCCAAAGAT 
GTCAAACCCC CAGACCTCTG GATCCATCAC GAGAGACTGG AGCTCAAACC CATTGATAAG 
TCTCCAGACC CAAACCCCAT CATGACTGAT ACACCAATTC CTCGTAATTC TCAAGATATC 
ACACCAGTTG ACAATTCCAT GGACAGCAAC ATCCATCAAA GGCGGAATTC ATATAGAGGG 
CATGAATCAG AGGACAGCAT GTCTACACTG GCAGGAAGGC GGGGAATGAG ACCAAAAATG 
ATGATGCCCT TTGACTCCCA GCCACCCCAG CCTGTGATTA GTGCCCATCC CATCCATTCC 
CTCGATAACC CTCACCATCA TTTCCACTCC AGCAGCCTCG CTTCTCCAGC TCGCAGTCAT 
CTCTACCACC CGGGCAGCCC ATGGCCCATT GGCACATCCA TGTCCCTTTC AGACAGGGCC 
AATTCCACAG AATCTGTTCG AAACACCCCC AGCACTGACA CCATGCCAGC CTCCTCGTCT 
CAAACATGCT GTACCGATCA TCAGGATCCT GAAGGTGCTA CCAGCTCTTC TTACTTGGCC 
AGCTCCCAAG AGGAAGATTC GGGCCAGAGT CTCCCTACAG CCCACGTTCG CCCTTCCCAC 
CCACTGAAGA GCTTTGCTGT GCCGGCAATC CCACCCACCG GGCCTCCCAC CTACGATCCA 
GCGTTGCCAA GCACACCGTT ACTCTCCCAG CAAGCTCTGA ATCATCACAT CCACGCCGTG 
AAGACGGCCT CCATCGGGAC TTTAGGAAGG AGCCGGCCTC CTATGCCAGT GGTGGTTCCC 
AGTGCCCCTG AAGTGCAGGA GACCACAAGG ATGCTGGAAG ACTCCGAGAG TAGCTATGAA 
CCAGATGAGC TGACCAAAGA GATGGCCCAC CTGGAAGGAC TGATGAAGGA CCTAAACGCC 
ATCACGACAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808328.1
CDS	61..4455
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010810026.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810026.2

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GTGCCAGATC CCACTCCCAT GATGCCACCA 
GTGGGAGTTC AGGCTTCCAT TCTGAGCCAT GACACCATAA GGATCACCTG GGCGGACAAC 
TCCCTGCCCA AACACCAGAA GATCACAGAC TCCCGATACT ACACAGTCCG ATGGAAAACC 
AACATCCCAG CAAACACCAA GTACAAGAAT GCTAATGCAA CGACTTTGAG TTATTTGGTG 
ACTGGTTTAA AACCAAATAC ACTCTATGAA TTCTCTGTGA TGGTGACCAA AGGTCGAAGA 
TCAAGCACAT GGAGCATGAC AGCCCACGGG ACTACTTTTG AATTAGTTCC TACTTCTCCG 
CCCAAGGATG TGACAGTTGT GAGTAAAGAG GGGAAACCTA GGACCATAAT TGTAAATTGG 
CAGCCTCCCT CTGAAGCCAA TGGCAAGATT ACAGGTTACA TCATATATTA CAGCACGGAT 
GTGAATGCAG AGATACATGA CTGGGTTATC GAGCCTGTTG TGGGAAACAG GCTGACTCAC 
CAGATACAGG AATTAACACT TGACACACCA TACTACTTCA AAATCCAAGC CCGGAACTCA 
AAGGGCATGG GGCCCATGTC TGAAGCTGTC CAGTTCAGAA CACCCAAAGC GGACTCCTCT 
GATAAAATGG CTAATGATCA AGCCTCGGGG TCTGCAGGGA AAGGAAGCCG ACTGCCAGAC 
CTGGGATCCG ACTACAAACC TCCAATGAGC GGCAGTAACA GCCCTCATGG AAGCCCCACC 
TCTCCTCTGG ACAGTAACAT GCTGCTGGTC ATCATCGTTT CCGTTGGTGT CATCACCATC 
GTGGTGGTCG TGATCATTGC TGTTTTCTGC ACCCGGCGGA CTACCTCTCA CCAGAAAAAG 
AAACGAGCTG CTTGCAAATC AGTGAATGGC TCCCACAAGT ATAAAGGGAA CTCCAAAGAT 
GTCAAACCCC CAGACCTCTG GATCCATCAC GAGAGACTGG AGCTCAAACC CATTGATAAG 
TCTCCAGACC CAAACCCCAT CATGACTGAT ACACCAATTC CTCGTAATTC TCAAGATATC 
ACACCAGTTG ACAATTCCAT GGACAGCAAC ATCCATCAAA GGCGGAATTC ATATAGAGGG 
CATGAATCAG AGGACAGCAT GTCTACACTG GCAGGAAGGC GGGGAATGAG ACCAAAAATG 
ATGATGCCCT TTGACTCCCA GCCACCCCAG CCTGTGATTA GTGCCCATCC CATCCATTCC 
CTCGATAACC CTCACCATCA TTTCCACTCC AGCAGCCTCG CTTCTCCAGC TCGCAGTCAT 
CTCTACCACC CGGGCAGCCC ATGGCCCATT GGCACATCCA TGTCCCTTTC AGACAGGGCC 
AATTCCACAG AATCTGTTCG AAACACCCCC AGCACTGACA CCATGCCAGC CTCCTCGTCT 
CAAACATGCT GTACCGATCA TCAGGATCCT GAAGGTGCTA CCAGCTCTTC TTACTTGGCC 
AGCTCCCAAG AGGAAGATTC GGGCCAGAGT CTCCCTACAG CCCACGTTCG CCCTTCCCAC 
CCACTGAAGA GCTTTGCTGT GCCGGCAATC CCACCCACCG GGCCTCCCAC CTACGATCCA 
GCGTTGCCAA GCACACCGTT ACTCTCCCAG CAAGCTCTGA ATCATCACAT CCACGCCGTG 
AAGACGGCCT CCATCGGGAC TTTAGGAAGG AGCCGGCCTC CTATGCCAGT GGTGGTTCCC 
AGTGCCCCTG AAGTGCAGGA GACCACAAGG ATGCTGGAAG ACTCCGAGAG TAGCTATGAA 
CCAGATGAGC TGACCAAAGA GATGGCCCAC CTGGAAGGAC TGATGAAGGA CCTAAACGCC 
ATCACGACAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010809030.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807332.1 Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.

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CloneID	OBa59382
Clone ID Related Accession (Same CDS sequence)	XM_010809030.2 , XM_010810028.2
Accession Version	XM_010809030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCA ATCTGTTCGA AACACCCCCA GCACTGACAC CATGCCAGCC 
TCCTCGTCTC AAACATGCTG TACCGATCAT CAGGATCCTG AAGGTGCTAC CAGCTCTTCT 
TACTTGGCCA GCTCCCAAGA GGAAGATTCG GGCCAGAGTC TCCCTACAGC CCACGTTCGC 
CCTTCCCACC CACTGAAGAG CTTTGCTGTG CCGGCAATCC CACCCACCGG GCCTCCCACC 
TACGATCCAG CGTTGCCAAG CACACCGTTA CTCTCCCAGC AAGCTCTGAA TCATCACATC 
CACGCCGTGA AGACGGCCTC CATCGGGACT TTAGGAAGGA GCCGGCCTCC TATGCCAGTG 
GTGGTTCCCA GTGCCCCTGA AGTGCAGGAG ACCACAAGGA TGCTGGAAGA CTCCGAGAGT 
AGCTATGAAC CAGATGAGCT GACCAAAGAG ATGGCCCACC TGGAAGGACT GATGAAGGAC 
CTAAACGCCA TCACGACAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807332.1
CDS	62..4345
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010809030.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809030.2

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCA ATCTGTTCGA AACACCCCCA GCACTGACAC CATGCCAGCC 
TCCTCGTCTC AAACATGCTG TACCGATCAT CAGGATCCTG AAGGTGCTAC CAGCTCTTCT 
TACTTGGCCA GCTCCCAAGA GGAAGATTCG GGCCAGAGTC TCCCTACAGC CCACGTTCGC 
CCTTCCCACC CACTGAAGAG CTTTGCTGTG CCGGCAATCC CACCCACCGG GCCTCCCACC 
TACGATCCAG CGTTGCCAAG CACACCGTTA CTCTCCCAGC AAGCTCTGAA TCATCACATC 
CACGCCGTGA AGACGGCCTC CATCGGGACT TTAGGAAGGA GCCGGCCTCC TATGCCAGTG 
GTGGTTCCCA GTGCCCCTGA AGTGCAGGAG ACCACAAGGA TGCTGGAAGA CTCCGAGAGT 
AGCTATGAAC CAGATGAGCT GACCAAAGAG ATGGCCCACC TGGAAGGACT GATGAAGGAC 
CTAAACGCCA TCACGACAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010810028.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808330.1 Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.

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CloneID	OBa59382
Clone ID Related Accession (Same CDS sequence)	XM_010809030.2 , XM_010810028.2
Accession Version	XM_010810028.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCA ATCTGTTCGA AACACCCCCA GCACTGACAC CATGCCAGCC 
TCCTCGTCTC AAACATGCTG TACCGATCAT CAGGATCCTG AAGGTGCTAC CAGCTCTTCT 
TACTTGGCCA GCTCCCAAGA GGAAGATTCG GGCCAGAGTC TCCCTACAGC CCACGTTCGC 
CCTTCCCACC CACTGAAGAG CTTTGCTGTG CCGGCAATCC CACCCACCGG GCCTCCCACC 
TACGATCCAG CGTTGCCAAG CACACCGTTA CTCTCCCAGC AAGCTCTGAA TCATCACATC 
CACGCCGTGA AGACGGCCTC CATCGGGACT TTAGGAAGGA GCCGGCCTCC TATGCCAGTG 
GTGGTTCCCA GTGCCCCTGA AGTGCAGGAG ACCACAAGGA TGCTGGAAGA CTCCGAGAGT 
AGCTATGAAC CAGATGAGCT GACCAAAGAG ATGGCCCACC TGGAAGGACT GATGAAGGAC 
CTAAACGCCA TCACGACAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808330.1
CDS	62..4345
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQQSVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010810028.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810028.2

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGGGCA TGAATCAGAG 
GACAGCATGT CTACACTGGC AGGAAGGCGG GGAATGAGAC CAAAAATGAT GATGCCCTTT 
GACTCCCAGC CACCCCAGCA ATCTGTTCGA AACACCCCCA GCACTGACAC CATGCCAGCC 
TCCTCGTCTC AAACATGCTG TACCGATCAT CAGGATCCTG AAGGTGCTAC CAGCTCTTCT 
TACTTGGCCA GCTCCCAAGA GGAAGATTCG GGCCAGAGTC TCCCTACAGC CCACGTTCGC 
CCTTCCCACC CACTGAAGAG CTTTGCTGTG CCGGCAATCC CACCCACCGG GCCTCCCACC 
TACGATCCAG CGTTGCCAAG CACACCGTTA CTCTCCCAGC AAGCTCTGAA TCATCACATC 
CACGCCGTGA AGACGGCCTC CATCGGGACT TTAGGAAGGA GCCGGCCTCC TATGCCAGTG 
GTGGTTCCCA GTGCCCCTGA AGTGCAGGAG ACCACAAGGA TGCTGGAAGA CTCCGAGAGT 
AGCTATGAAC CAGATGAGCT GACCAAAGAG ATGGCCCACC TGGAAGGACT GATGAAGGAC 
CTAAACGCCA TCACGACAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010809031.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807333.1 Bos taurus neogenin 1 (NEO1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa59383
Clone ID Related Accession (Same CDS sequence)	XM_010809031.2 , XM_010810034.2
Accession Version	XM_010809031.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGAATC TGTTCGAAAC 
ACCCCCAGCA CTGACACCAT GCCAGCCTCC TCGTCTCAAA CATGCTGTAC CGATCATCAG 
GATCCTGAAG GTGCTACCAG CTCTTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCGGGC 
CAGAGTCTCC CTACAGCCCA CGTTCGCCCT TCCCACCCAC TGAAGAGCTT TGCTGTGCCG 
GCAATCCCAC CCACCGGGCC TCCCACCTAC GATCCAGCGT TGCCAAGCAC ACCGTTACTC 
TCCCAGCAAG CTCTGAATCA TCACATCCAC GCCGTGAAGA CGGCCTCCAT CGGGACTTTA 
GGAAGGAGCC GGCCTCCTAT GCCAGTGGTG GTTCCCAGTG CCCCTGAAGT GCAGGAGACC 
ACAAGGATGC TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG 
GCCCACCTGG AAGGACTGAT GAAGGACCTA AACGCCATCA CGACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010807333.1
CDS	63..4253
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010809031.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010809031.2

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGAATC TGTTCGAAAC 
ACCCCCAGCA CTGACACCAT GCCAGCCTCC TCGTCTCAAA CATGCTGTAC CGATCATCAG 
GATCCTGAAG GTGCTACCAG CTCTTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCGGGC 
CAGAGTCTCC CTACAGCCCA CGTTCGCCCT TCCCACCCAC TGAAGAGCTT TGCTGTGCCG 
GCAATCCCAC CCACCGGGCC TCCCACCTAC GATCCAGCGT TGCCAAGCAC ACCGTTACTC 
TCCCAGCAAG CTCTGAATCA TCACATCCAC GCCGTGAAGA CGGCCTCCAT CGGGACTTTA 
GGAAGGAGCC GGCCTCCTAT GCCAGTGGTG GTTCCCAGTG CCCCTGAAGT GCAGGAGACC 
ACAAGGATGC TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG 
GCCCACCTGG AAGGACTGAT GAAGGACCTA AACGCCATCA CGACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEO1 ( XM_010810034.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808336.1 Bos taurus neogenin 1 (NEO1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa59383
Clone ID Related Accession (Same CDS sequence)	XM_010809031.2 , XM_010810034.2
Accession Version	XM_010810034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	neogenin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGAATC TGTTCGAAAC 
ACCCCCAGCA CTGACACCAT GCCAGCCTCC TCGTCTCAAA CATGCTGTAC CGATCATCAG 
GATCCTGAAG GTGCTACCAG CTCTTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCGGGC 
CAGAGTCTCC CTACAGCCCA CGTTCGCCCT TCCCACCCAC TGAAGAGCTT TGCTGTGCCG 
GCAATCCCAC CCACCGGGCC TCCCACCTAC GATCCAGCGT TGCCAAGCAC ACCGTTACTC 
TCCCAGCAAG CTCTGAATCA TCACATCCAC GCCGTGAAGA CGGCCTCCAT CGGGACTTTA 
GGAAGGAGCC GGCCTCCTAT GCCAGTGGTG GTTCCCAGTG CCCCTGAAGT GCAGGAGACC 
ACAAGGATGC TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG 
GCCCACCTGG AAGGACTGAT GAAGGACCTA AACGCCATCA CGACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808336.1
CDS	63..4253
Translation	MAAEPGAWRLLSTSFWLCCLLLLGRRAPGVAAARSGSPPQSSGSSIRTFTPFYFLVEPVDTLSVRGSSVTLNCSAYSEPSPKIEWKKDGTLLNLVSDDRRQLLPDGSLFISSVVHSKHNKPDEGYYQCVATVESLGTIVSRTAKLTVAGLPRFASQPEPSSVYVGNSAVLNCEVNADLVPFVRWEQNKQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCMVESGGPPKYSEEAELKVLPDPGATSNLVFLKQPSSLIRVIGQSAVLPCVASGLPTPSIRWVKNQETVDTESSGRLVLLAGGSLEIDDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATAGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSRPGEMQVTIQNLMPATVYIFRVMAQNKHGPGESSAPLRVETQPEVQLPGPAPNIRACATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTFSDVPSAAPQNLSLEVRNSKSIVIHWQPPPPAAQNGEITGYKVRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTVNGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMANDQASGSAGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRESVRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPTGPPTYDPALPSTPLLSQQALNHHIHAVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_010810034.2
Organism	Bos taurus(cattle)
Definition	Bos taurus neogenin 1 (NEO1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810034.2

ORF Insert Sequence:

ATGGCGGCGG AGCCGGGAGC CTGGCGACTC CTCAGCACTT CCTTCTGGCT CTGCTGCCTG 
CTGCTGCTCG GGCGCCGGGC GCCAGGCGTC GCGGCCGCCA GGAGCGGCTC CCCGCCGCAG 
TCCTCAGGAT CCAGCATTCG GACATTCACT CCATTTTACT TTTTGGTGGA ACCAGTGGAT 
ACACTCTCAG TTAGAGGCTC TTCTGTTACA CTAAACTGTT CAGCATATTC TGAGCCATCT 
CCAAAAATTG AATGGAAAAA AGATGGAACT CTTTTAAACT TAGTGTCAGA TGATCGACGC 
CAGCTTCTCC CAGATGGGTC TTTGTTTATC AGCAGTGTGG TGCATTCCAA ACACAATAAA 
CCTGATGAAG GTTATTATCA GTGTGTAGCC ACTGTTGAGA GTCTTGGAAC TATTGTCAGC 
AGAACGGCCA AGCTCACAGT AGCAGGTCTT CCAAGATTTG CCAGCCAACC AGAGCCTTCT 
TCAGTTTACG TTGGGAACAG TGCGGTTCTG AATTGTGAAG TTAATGCAGA TTTGGTCCCA 
TTTGTGAGAT GGGAACAGAA CAAACAGCCT CTTCTTCTGG ATGATAGAGT TATCAAACTT 
CCAAGCGGAA TGCTAGTTAT CAGCAACGCA ACTGAAGGAG ATGGGGGACT CTATCGATGC 
ATGGTTGAAA GTGGAGGGCC ACCAAAGTAT AGTGAGGAAG CTGAATTAAA AGTTCTTCCA 
GATCCTGGTG CCACATCAAA CTTGGTATTT TTGAAACAAC CTTCTTCCTT AATCAGAGTC 
ATTGGTCAGA GTGCAGTGTT GCCATGTGTT GCTTCAGGAC TTCCTACTCC AAGCATTAGA 
TGGGTGAAAA ACCAGGAGAC AGTTGACACA GAAAGCTCTG GAAGGTTGGT ATTGCTGGCA 
GGTGGTAGTC TGGAGATCGA TGATGTCACC GAGGATGATG CTGGGACTTA TTTTTGTATA 
GCTGATAATG GAAATGAGAC CATTGAAGCT CAAGCAGAGC TTACAGTACA AGCCCAACCT 
GAATTCCTGA AGCAGCCTAC TAATATATAT GCTCATGAAT CTATGGATAT TGTGTTTGAA 
TGTGAAGTCA CTGGAAAGCC AACTCCAACT GTGAAGTGGG TCAAGAATGG GGATATGGTT 
ATCCCAAGTG ATTACTTTAA GATTGTAAAG GAACATAATC TTCAAGTTTT GGGTCTGGTG 
AAATCAGATG AAGGGTTCTA TCAATGCATT GCTGAAAATG ATGTTGGAAA TGCACAAGCT 
GGAGCCCAGC TGATAATCCT TGAACATGAT GTTGCCATCC CAACATTACC TCCCACTTCA 
CTGACCAGTG CCACTACTGA CCATCTAGCA CCAGCCACAG CTGGACCACT GCCTTCAGCC 
CCACGGGATG TCGTGGCCTC CCTGGTCTCT ACCCGCTTCA TCAAACTGAC GTGGCGGACA 
CCTGCATCCG ATCCTCATGG GGACAACCTT ACCTACTCTG TCTTCTACAC CAAGGAGGGG 
ATTGCTAGGG AACGTGTTGA GAATACCAGT CGCCCAGGAG AAATGCAGGT GACCATTCAA 
AACCTAATGC CAGCAACAGT GTACATCTTT AGAGTTATGG CTCAAAATAA GCATGGCCCT 
GGAGAGAGTT CAGCTCCACT GCGGGTAGAA ACACAGCCTG AGGTTCAGCT CCCTGGCCCA 
GCACCTAACA TCCGAGCCTG TGCAACTTCA CCGACTTCTA TCACTGTCAC CTGGGAAACA 
CCGTTGTCTG GAAATGGGGA AATTCAGAAT TACAAACTGT ACTACATGGA AAAGGGGACT 
GATAAAGAAC AGGATGTTGA TGTTTCGAGT CACTCCCACA CCATTAACGG ATTGAAAAAA 
TACACGGAGT ATAGTTTCCG AGTGGTGGCC TACAATAAAC ATGGTCCTGG AGTCTCCACA 
CAAGATGTTG CTGTTCGAAC TTTTTCAGAT GTTCCCAGTG CTGCTCCTCA GAATCTGTCT 
TTAGAAGTAA GAAATTCAAA GAGTATTGTG ATTCACTGGC AGCCACCTCC TCCAGCCGCA 
CAAAATGGAG AGATCACTGG CTACAAGGTT CGCTACCGAA AGGCTTCCCG AAAGAGTGAT 
GTCACTGAGA CCTTGGTGTC TGGGACACAG CTGTCACAGC TGATTGAAGG TCTTGATCGG 
GGAACTGAAT ATAATTTCCG AGTGGCTGCT CTAACAGTCA ATGGTACAGG CCCAGCTACT 
GACTGGCTGT CAGCTGAAAC TTTTGAAAGT GACTTAGATG AAACTCGTGT TCCAGAAGTG 
CCTAGTTCTC TTCACGTCCG CCCACTGGTC ACTAGCATCG TAGTAAGCTG GACGCCTCCA 
GAGAATCAAA ACATTGTGGT CAGAGGTTAT GCCATTGGTT ATGGCATTGG CAGCCCTCAT 
GCCCAGACCA TAAAGGTAGA CTATAAACAA CGCTATTACA CCATCGAAAA TCTGGATCCC 
AGCTCTCACT ATGTGATTAC CCTGAAAGCA TTTAACAACG TAGGTGAAGG CATTCCCTTG 
TACGAGAGTG CTGTGACCAG ACCTCACACA GACACTTCTG AAGTTGATTT GTTTGTTATT 
AATGCTCCAT ACACTCCAGT GCCAGATCCC ACTCCCATGA TGCCACCAGT GGGAGTTCAG 
GCTTCCATTC TGAGCCATGA CACCATAAGG ATCACCTGGG CGGACAACTC CCTGCCCAAA 
CACCAGAAGA TCACAGACTC CCGATACTAC ACAGTCCGAT GGAAAACCAA CATCCCAGCA 
AACACCAAGT ACAAGAATGC TAATGCAACG ACTTTGAGTT ATTTGGTGAC TGGTTTAAAA 
CCAAATACAC TCTATGAATT CTCTGTGATG GTGACCAAAG GTCGAAGATC AAGCACATGG 
AGCATGACAG CCCACGGGAC TACTTTTGAA TTAGTTCCTA CTTCTCCGCC CAAGGATGTG 
ACAGTTGTGA GTAAAGAGGG GAAACCTAGG ACCATAATTG TAAATTGGCA GCCTCCCTCT 
GAAGCCAATG GCAAGATTAC AGGTTACATC ATATATTACA GCACGGATGT GAATGCAGAG 
ATACATGACT GGGTTATCGA GCCTGTTGTG GGAAACAGGC TGACTCACCA GATACAGGAA 
TTAACACTTG ACACACCATA CTACTTCAAA ATCCAAGCCC GGAACTCAAA GGGCATGGGG 
CCCATGTCTG AAGCTGTCCA GTTCAGAACA CCCAAAGCGG ACTCCTCTGA TAAAATGGCT 
AATGATCAAG CCTCGGGGTC TGCAGGGAAA GGAAGCCGAC TGCCAGACCT GGGATCCGAC 
TACAAACCTC CAATGAGCGG CAGTAACAGC CCTCATGGAA GCCCCACCTC TCCTCTGGAC 
AGTAACATGC TGCTGGTCAT CATCGTTTCC GTTGGTGTCA TCACCATCGT GGTGGTCGTG 
ATCATTGCTG TTTTCTGCAC CCGGCGGACT ACCTCTCACC AGAAAAAGAA ACGAGCTGCT 
TGCAAATCAG TGAATGGCTC CCACAAGTAT AAAGGGAACT CCAAAGATGT CAAACCCCCA 
GACCTCTGGA TCCATCACGA GAGACTGGAG CTCAAACCCA TTGATAAGTC TCCAGACCCA 
AACCCCATCA TGACTGATAC ACCAATTCCT CGTAATTCTC AAGATATCAC ACCAGTTGAC 
AATTCCATGG ACAGCAACAT CCATCAAAGG CGGAATTCAT ATAGAGAATC TGTTCGAAAC 
ACCCCCAGCA CTGACACCAT GCCAGCCTCC TCGTCTCAAA CATGCTGTAC CGATCATCAG 
GATCCTGAAG GTGCTACCAG CTCTTCTTAC TTGGCCAGCT CCCAAGAGGA AGATTCGGGC 
CAGAGTCTCC CTACAGCCCA CGTTCGCCCT TCCCACCCAC TGAAGAGCTT TGCTGTGCCG 
GCAATCCCAC CCACCGGGCC TCCCACCTAC GATCCAGCGT TGCCAAGCAC ACCGTTACTC 
TCCCAGCAAG CTCTGAATCA TCACATCCAC GCCGTGAAGA CGGCCTCCAT CGGGACTTTA 
GGAAGGAGCC GGCCTCCTAT GCCAGTGGTG GTTCCCAGTG CCCCTGAAGT GCAGGAGACC 
ACAAGGATGC TGGAAGACTC CGAGAGTAGC TATGAACCAG ATGAGCTGAC CAAAGAGATG 
GCCCACCTGG AAGGACTGAT GAAGGACCTA AACGCCATCA CGACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEO1
Entrez Gene ID	100297058
Full Name	neogenin 1
General protein information	Preferred Names neogenin 1 Names neogenin neogenin homolog 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Danio rerio (zebrafish)	neo1	NP_775325.1
Canis lupus familiaris (dog)	NEO1	XP_005638634.1
Bos taurus (cattle)	NEO1	XP_005211488.1
Mus musculus (house mouse)	Neo1	NP_032710.2
Drosophila melanogaster (fruit fly)	fra	NP_725185.1
Homo sapiens (human)	NEO1	NP_002490.2
Pan troglodytes (chimpanzee)	NEO1	XP_510660.3
	NEO1	XP_002804901.1
Rattus norvegicus (Norway rat)	Neo1	XP_006243248.1
Gallus gallus (chicken)	NEO1	XP_004943713.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006083	XP_316139.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEO1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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