TOPAZ1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOPAZ1
Entrez Gene ID	100296400
Full Name	testis and ovary specific PAZ domain containing 1
Synonyms	C22H3orf77
General protein information	Preferred Names testis and ovary specific PAZ domain containing 1 Names testis- and ovary-specific PAZ domain-containing protein 1 testis and ovary-specific PAZ domain gene 1 topaz 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001252441.1	NP_001239370.1	protein TOPAZ1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TOPAZ1	NP_001138502.1
Mus musculus (house mouse)	Topaz1	NP_001186665.1
Bos taurus (cattle)	TOPAZ1	NP_001239370.1
Rattus norvegicus (Norway rat)	Topaz1	XP_006244266.1
Pan troglodytes (chimpanzee)	TOPAZ1	XP_526186.2
Xenopus tropicalis (tropical clawed frog)	topaz1	NP_001186868.1
	TOPAZ1	XP_001114967.1
Canis lupus familiaris (dog)	TOPAZ1	XP_003433200.2
Gallus gallus (chicken)	TOPAZ1	XP_003640786.2

Gene Ontology
Bibliography

Related articles in PubMed

TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates.
Baillet A, Le Bouffant R, Volff JN, Luangpraseuth A, Poumerol E, Th?pot D, Pailhoux E, Livera G, Cotinot C, Mandon-P?pin B
PloS one6(11)e26950(2011)

The genome sequence of taurine cattle: a window to ruminant biology and evolution.
, Elsik CG, Tellam RL, Worley KC, Gibbs RA, Muzny DM, Weinstock GM, Adelson DL, Eichler EE, Elnitski L, Guig? R, Hamernik DL, Kappes SM, Lewin HA, Lynn DJ, Nicholas FW, Reymond A, Rijnkels M, Skow LC, Zdobnov EM, Schook L, Womack J, Alioto T, Antonarakis SE, Astashyn A, Chapple CE, Chen HC, Chrast J, C?mara F, Ermolaeva O, Henrichsen CN, Hlavina W, Kapustin Y, Kiryutin B, Kitts P, Kokocinski F, Landrum M, Maglott D, Pruitt K, Sapojnikov V, Searle SM, Solovyev V, Souvorov A, Ucla C, Wyss C, Anzola JM, Gerlach D, Elhaik E, Graur D, Reese JT, Edgar RC, McEwan JC, Payne GM, Raison JM, Junier T, Kriventseva EV, Eyras E, Plass M, Donthu R, Larkin DM, Reecy J, Yang MQ, Chen L, Cheng Z, Chitko-McKown CG, Liu GE, Matukumalli LK, Song J, Zhu B, Bradley DG, Brinkman FS, Lau LP, Whiteside MD, Walker A, Wheeler TT, Casey T, German JB, Lemay DG, Maqbool NJ, Molenaar AJ, Seo S, Stothard P, Baldwin CL, Baxter R, Brinkmeyer-Langford CL, Brown WC, Childers CP, Connelley T, Ellis SA, Fritz K, Glass EJ, Herzig CT, Iivanainen A, Lahmers KK, Bennett AK, Dickens CM, Gilbert JG, Hagen DE, Salih H, Aerts J, Caetano AR, Dalrymple B, Garcia JF, Gill CA, Hiendleder SG, Memili E, Spurlock D, Williams JL, Alexander L, Brownstein MJ, Guan L, Holt RA, Jones SJ, Marra MA, Moore R, Moore SS, Roberts A, Taniguchi M, Waterman RC, Chacko J, Chandrabose MM, Cree A, Dao MD, Dinh HH, Gabisi RA, Hines S, Hume J, Jhangiani SN, Joshi V, Kovar CL, Lewis LR, Liu YS, Lopez J, Morgan MB, Nguyen NB, Okwuonu GO, Ruiz SJ, Santibanez J, Wright RA, Buhay C, Ding Y, Dugan-Rocha S, Herdandez J, Holder M, Sabo A, Egan A, Goodell J, Wilczek-Boney K, Fowler GR, Hitchens ME, Lozado RJ, Moen C, Steffen D, Warren JT, Zhang J, Chiu R, Schein JE, Durbin KJ, Havlak P, Jiang H, Liu Y, Qin X, Ren Y, Shen Y, Song H, Bell SN, Davis C, Johnson AJ, Lee S, Nazareth LV, Patel BM, Pu LL, Vattathil S, Williams RL Jr, Curry S, Hamilton C, Sodergren E, Wheeler DA, Barris W, Bennett GL, Eggen A, Green RD, Harhay GP, Hobbs M, Jann O, Keele JW, Kent MP, Lien S, McKay SD, McWilliam S, Ratnakumar A, Schnabel RD, Smith T, Snelling WM, Sonstegard TS, Stone RT, Sugimoto Y, Takasuga A, Taylor JF, Van Tassell CP, Macneil MD, Abatepaulo AR, Abbey CA, Ahola V, Almeida IG, Amadio AF, Anatriello E, Bahadue SM, Biase FH, Boldt CR, Carroll JA, Carvalho WA, Cervelatti EP, Chacko E, Chapin JE, Cheng Y, Choi J, Colley AJ, de Campos TA, De Donato M, Santos IK, de Oliveira CJ, Deobald H, Devinoy E, Donohue KE, Dovc P, Eberlein A, Fitzsimmons CJ, Franzin AM, Garcia GR, Genini S, Gladney CJ, Grant JR, Greaser ML, Green JA, Hadsell DL, Hakimov HA, Halgren R, Harrow JL, Hart EA, Hastings N, Hernandez M, Hu ZL, Ingham A, Iso-Touru T, Jamis C, Jensen K, Kapetis D, Kerr T, Khalil SS, Khatib H, Kolbehdari D, Kumar CG, Kumar D, Leach R, Lee JC, Li C, Logan KM, Malinverni R, Marques E, Martin WF, Martins NF, Maruyama SR, Mazza R, McLean KL, Medrano JF, Moreno BT, Mor? DD, Muntean CT, Nandakumar HP, Nogueira MF, Olsaker I, Pant SD, Panzitta F, Pastor RC, Poli MA, Poslusny N, Rachagani S, Ranganathan S, Razpet A, Riggs PK, Rincon G, Rodriguez-Osorio N, Rodriguez-Zas SL, Romero NE, Rosenwald A, Sando L, Schmutz SM, Shen L, Sherman L, Southey BR, Lutzow YS, Sweedler JV, Tammen I, Telugu BP, Urbanski JM, Utsunomiya YT, Verschoor CP, Waardenberg AJ, Wang Z, Ward R, Weikard R, Welsh TH Jr, White SN, Wilming LG, Wunderlich KR, Yang J, Zhao FQ
Science (New York, N.Y.)324(5926)522-8(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TOPAZ1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPAZ1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01281	NM_001252441.1 Latest version!	Bos taurus testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOPAZ1 ( NM_001252441.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001239370.1 Bos taurus testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.

Price & Availability↑

CloneID	OBa01281
Clone ID Related Accession (Same CDS sequence)	NM_001252441.1
Accession Version	NM_001252441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Bos taurus(cattle)
Product	protein TOPAZ1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BK008402.1. On or before Dec 7, 2011 this sequence version replaced XM_003583791.1, XM_003587613.1. ##Evidence-Data-START## Transcript exon combination :: BK008402.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCGGG CTCCACCCTC GGGCCCCACG ACGGCCCCGG GGCGTGAAGA CGACGCTCGA 
GGCCTGAGGA AGAGAAGCCG GCGGCCAGGG CTAGGCGAGG CAGGAGGCTG CGGCCCTGAG 
GCGGAGGGGC TCGAGGAGAG CAGGCAAAAG AGGAGGATGG TGGCTCGGGC GTCTGGGAGA 
GAAGAGGTGG AAAGTGACAA GTCAGCCAAG GAAAAAAGAA AAGTTACTGA AGCCTCAAGT 
GATGATCCAC AGCCAGGGAT TGACCTGGTA AGAAAGGAAT CATTAACCAG TTCGGAATCT 
TTCCCAACAG TGGAATGCAG TGAATTTCAA AGTATGGCTT TCTTGCAGTC TTTGGGTAAG 
GAAAGGTTGG TAGAAGGTAT TAAAAGAAGA ATTCGAATTA AAAAATGTAA AAGCTTAGAA 
AGCCCAGCTC TCAAAATGAC TGAAAATAAG GCTACACAAA ACAGTAAGGT TGAATTCCAA 
GATGAACTGT ACAAGAATAC TCTGAAATAT TCTTGTAACA GCTTGTCACC TGGAGTAGAA 
AATAATTCTG TTTTAAAATT ACATGATTGC AGTTGTCTCT CCCATTCCAA GGATTGTAAT 
GATGAAAATA ACCTTGCATA TAAACCTGAT GGAGGATGTA TGCATGTACC AGAAAATTCC 
TCAAAGTCAA AGAAAGAAAA CCCTAGGAGT CTCATAGATA AGACTGATCC AAGCAATATT 
CCTCAGCTTT TACAAACTGA AGAAAACGTA ATGCGAGTAA GTCAGTTATT GCTTGAAGAA 
AATGATTCGT ACCTAAGTAA AAATAATGGC TTATTTTCCT GCCTTCAAAG TGAAAAAACT 
AAACATTCAA TAGAGGAGAG CAGTATTGGG AGAAAATCCA GAAAAAGGAT GAAGGTGTCT 
GAAAAAGGAA ATGGAATGGT TATTGAGATG AAGTTTTCTA ATATGTGTAA CAAATCTGAG 
CTGATGTTAC AAGGAAACCA AACAGGTGCT GATGGTAAAG AAACAGAGAT TTTAGAAGCT 
AAAAAAAGTT CTTTGAAAGT TTTGAGAAAA GTAAATAATA ATACGCTGTC ACCAATGGAT 
CCTTTATTAA GTCTTCCAGA AACAGGGAAA AAAACAAGTC CTGAACATTG TGCAAATGCT 
GTGTTTCAGA AAGCCCTTGA GCAACTTTCA AAAGAAGAAA CAAAGAATGT TTCACAGCCT 
TTAGGATGTA CCAGCATGGA TCCACCAGAA GATTATTTTA AGTCAATGAA AAACTCATTA 
GTGAAGTCAC TTAGTGATTG CTTTCCTATT GAAAAGAGGT CTTCTAGGGA AAGCTTGAAG 
AATGAAGCTG AAGAATCTAA ATATAGCTGT CAAAGAACAA TACCAATGAC TGGCAAAAGA 
ACTTGGCCTT GTTATTCATG TGCTAGAATA TCTGCCCAGT GTTGGAAAAA AGCTTCCTTG 
CCACAAAGTT CTAGGCAGGA TGATTTTCTT AAACATCAAA TGAATCAAAC TCACTTAACT 
GACTCCAAAT TAATGCTACA AAGTTCTGTA ACAGAAACAA ACAGTGCATC TTCAAGTATA 
GAAAAATTGG ATTCTAATTT AAATTGTTTA CCTTCAGTCT CTACAGTGGA ACCTACTTCA 
GTGGTTATAA AGGAACCTAT AGTCAATGAT GATGAAAAAA TGAAGTCAGA GGAACTAAGC 
AGAAGTGCAT CAGAGGTAGT TTCTAATACT ACTGAAGATA CTTCATTGAC TAATATGACT 
CACAACTTAA CAGGAAGTAA AAAAAAAGAT AGAGGGAATT TAACAAAACT GAATTTGACG 
GTGGCTTCCC AGGATGGTCA GGAAGCAAAT AACTCTACAA GCAAAACTGT TCATCGAAAA 
GCATGCATTA CTAAGCAAGC ACTGGTTGTT CCAGACCTAG TTAAGATACT GAACACAGGA 
CGGCTGACTA ATTTTAAAAT TCCTTTACTT AAGAACAAAA CAGAAAAAAG AAAAGAAATT 
AATGCCAAGT CATCAGAGAG AGAAGTATAT AGTCCCCTAG AACTTCTTGA CAGTGTATCG 
GGAGCAGAGG TAAGGCAGAG TAGGACTAAA GAAAATGCTG TCACAGTAAC TTCAGGACCT 
CAATCTTTGA GTATACAACG TAGTGTAATT CCAGTGCAAG CTAGTTCTGA CTCATTCTGC 
AGTAAGAATT CCTGTATTAT TGCTCCGAGC TTTTTAAAGC AAGGTAATAA TAAACAACCA 
TCTAACCACA TCTCTGCATC AGGCCGTATT ATTTCTAATA ATGCAGCTGG TTCTCTCACA 
GTTGAAAATA ATACATTTTC TTGTGATCCT GGATGTATAG AGAAAAATCC CACCTTCTAT 
TCCAATGAAC AAGAACCATT CAAAGCAGTT TCCTCTGAAG TTAGTGGTAG AAAAATGACT 
GAGAACTTTT CAGAAATTAA AGTGGGGTTT CCAGATATTC TGAAAGCATA TGAAGATGAT 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAACTCTCAT TTCCTTCAGA GGTTCCCATG ATAAGCCAGG AGCCCAATGT TGCTGAAGAG 
CATCAATCGA CAGACTCCAA GCACATGGAA CTTCCAGACA AAAAAGAACC AAGCAATGCC 
TTGAGGGAAC TGCCTGTACC GGATCCTGGA TCAATGAAGT CAGAGATCTG TGCTTCCTTG 
TCAGCAGCAA GTGAGATAAA ACATGATTCC AAAGATGCAA ACATTTCCTT AGGAGAAGTT 
ACTCATGAAA CTTCTTCAAA TGAAAAACCG AGAGGCCTAA GTGAACAGAC AAAAAGTTCA 
GACTTGGATG AAAAGTGTAG ATTTTCAGAC AAGGTAGCTA TTCGAGAAGA AAAAGAAACT 
ATTTCTGAAG TTTGCAGAAG GGATTCTAAA AATACAGAGA TTATGGTTGG TGAATGTCAC 
TTAGCAGCAC TGGTCTCCAA ACCTCTGTGC TTACCAGTGC CCTTGCCGCC TTTGAATCTT 
AATGCTCATC AGGAAGATAC ATTACTGAAT CCACAGATGA ATGATTTCAG ATTACCAGGG 
AAACATTCTG TCCTAAAGCT GCAGAATCCT GAAATTTGTG AAATATTTAA GAGGGAAAAA 
AATGTAGGGG TGTTCCAGAA GCCACTAGGG TTGATGATAC CCCACAGATA TTGCAAATTT 
CATTTTAATA CGTTACGTGG CTGTGAGCGA TCACAGTGCA AGTTTGGTCA CGTGCCTGAG 
CAAGGGGATG AAAAGGTTTG TATGGATGTG TTTAAGAAAT ATATAAGTAT CAATGAGCTG 
TGTTTGCTAC AACGTGCAGC AAACATGTTT ATGGAGTACT ACAGAAAGTT TCTTCCAGGT 
ATACACTTTG ATTTACAAGT ATTAAATGAC CTTCTAAGTT CTTTACTCAA ACACTGTTTA 
TTGAAAGAAG TATTTCAGGT CATGAACCTC TGCATAATGA TTAAGATGCT GCCTGCTCTG 
AAAATATTAC TAAAAATATT TGAGTATGTG GCAACTATGA AATTAAGGAA TGCTGTACCT 
GCTTTAATTA ATATCTTTTG CAAGCTCCTT GAAGCTGGGA TGGTACTTGA CCCAGAACAC 
TTTAACTATA TTGTTAAGCT TTTATACCAA GTACAGGCTT CCAAGCAAGA AATAACTGCA 
GTTCTGGAAA TGAAATCCAG GTTACACATG AGGCAATTTA AAAAGAACTG GAAATGTGAC 
TTAGAGGCAG CCTTGAATGA AATAGAGCAT TGTAAAGAAA AAGGTGACTG GACCAAACTG 
GGAAATGTAT ACCTTAACAT AAAAATGAGC TGTGAAAAAT TTGCAGATTT CCAGAGGTTT 
TGTGCTTGTA TTGCTGAAAC ACTAACAAAA GACTGTAAAG AAGAGCGACC AGGTGTTCCA 
TTTTGTGAAT TTGCTGAAAC AGTTAGCAAA GATCTGCAGA ATAGTGAAGT AGATAAAACT 
TTGCTGGGAA GAATCGGAAT CAGTGCTATG TACTTCTATC ACAAGCTGTT GCAGTGGTCC 
AAGGGAAGGA AGGTCTTAGA TAAACTATAT GAATTAAAAA TACATTTTAC AAGTTTAAAA 
GGACTTATAG GGCCTGAGAA GTTAGCACCA AGATGTCAAA TTGTGAATAT TGCAGCAGAA 
ATTTTTCTAA AAAGTGGAAG CCTAGATGGT GCCATTTGGG TATTAAGGGA GTCTGAGTGG 
ATCATTAATA CACCAGTATG GCCTTGCGAT CGACTGGATG TGCTCAATCG GCATAATCTG 
CTCTGTACAA TCGCACACGA AATCTTAGCG AAGAGTCTTT ACAGACAGAC ATTTGAAGTT 
TTGCAAAATC TACCAGGTTT TCAAAATTCT CAAGAAACTG TGGAGGTCTC ACAGTATAGC 
CTACTTTTTA ATAAACTTCT AGATGCCTGT ATAGAAAGCA ACAGCCTCGG TATGTCATCC 
TCTGTAGCAG AATTCATGAT TTCAAAGAGC ATCCCTATTG ATTTTTCCTT TCTTAGAAGA 
TTAATTACTT CTTTAGGAAG GAGTTGTTTA TGGCTCAAAG CCAGAGCCCA CTACAAAAGT 
GCTCTTTCAC TGGGTTGCTA CCCACCACTG GAAGGAAATT TGTACCGAAA ACTTCTCCTG 
ATTCCATCCT ATTTGTCTGA AATTGAAATG CTTTTAGCTA TTGAAATCTT CCTGGTATCT 
AATGCTAGTA GTATTCAGAG TCCTGGAACT TCTACACAGA TGCTGCAGAT AGTTTTAAAA 
AGATCTGAAG AAAACAAATC TCGGAGCAAG GATGATTATC AAGCTGCAGT GGAAAGATTA 
ATTACGGCTG CTCGTATATC AGATCCAAAG CTTTTCATTA AGCACATGAC TGTCAATGTT 
AATAAGGAAC AGGTTTATAG TTTGGAACAG TGTTCTGCCC TGAAATGGTT GAAAGAGAAT 
ATGAAGTGGG CCGGAAAGGT TTGGCTTTTC AATAACCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239370.1
CDS	1..4962
Translation	MPRAPPSGPTTAPGREDDARGLRKRSRRPGLGEAGGCGPEAEGLEESRQKRRMVARASGREEVESDKSAKEKRKVTEASSDDPQPGIDLVRKESLTSSESFPTVECSEFQSMAFLQSLGKERLVEGIKRRIRIKKCKSLESPALKMTENKATQNSKVEFQDELYKNTLKYSCNSLSPGVENNSVLKLHDCSCLSHSKDCNDENNLAYKPDGGCMHVPENSSKSKKENPRSLIDKTDPSNIPQLLQTEENVMRVSQLLLEENDSYLSKNNGLFSCLQSEKTKHSIEESSIGRKSRKRMKVSEKGNGMVIEMKFSNMCNKSELMLQGNQTGADGKETEILEAKKSSLKVLRKVNNNTLSPMDPLLSLPETGKKTSPEHCANAVFQKALEQLSKEETKNVSQPLGCTSMDPPEDYFKSMKNSLVKSLSDCFPIEKRSSRESLKNEAEESKYSCQRTIPMTGKRTWPCYSCARISAQCWKKASLPQSSRQDDFLKHQMNQTHLTDSKLMLQSSVTETNSASSSIEKLDSNLNCLPSVSTVEPTSVVIKEPIVNDDEKMKSEELSRSASEVVSNTTEDTSLTNMTHNLTGSKKKDRGNLTKLNLTVASQDGQEANNSTSKTVHRKACITKQALVVPDLVKILNTGRLTNFKIPLLKNKTEKRKEINAKSSEREVYSPLELLDSVSGAEVRQSRTKENAVTVTSGPQSLSIQRSVIPVQASSDSFCSKNSCIIAPSFLKQGNNKQPSNHISASGRIISNNAAGSLTVENNTFSCDPGCIEKNPTFYSNEQEPFKAVSSEVSGRKMTENFSEIKVGFPDILKAYEDDVLLIDVIQDDPDLFGVSNEGELSFPSEVPMISQEPNVAEEHQSTDSKHMELPDKKEPSNALRELPVPDPGSMKSEICASLSAASEIKHDSKDANISLGEVTHETSSNEKPRGLSEQTKSSDLDEKCRFSDKVAIREEKETISEVCRRDSKNTEIMVGECHLAALVSKPLCLPVPLPPLNLNAHQEDTLLNPQMNDFRLPGKHSVLKLQNPEICEIFKREKNVGVFQKPLGLMIPHRYCKFHFNTLRGCERSQCKFGHVPEQGDEKVCMDVFKKYISINELCLLQRAANMFMEYYRKFLPGIHFDLQVLNDLLSSLLKHCLLKEVFQVMNLCIMIKMLPALKILLKIFEYVATMKLRNAVPALINIFCKLLEAGMVLDPEHFNYIVKLLYQVQASKQEITAVLEMKSRLHMRQFKKNWKCDLEAALNEIEHCKEKGDWTKLGNVYLNIKMSCEKFADFQRFCACIAETLTKDCKEERPGVPFCEFAETVSKDLQNSEVDKTLLGRIGISAMYFYHKLLQWSKGRKVLDKLYELKIHFTSLKGLIGPEKLAPRCQIVNIAAEIFLKSGSLDGAIWVLRESEWIINTPVWPCDRLDVLNRHNLLCTIAHEILAKSLYRQTFEVLQNLPGFQNSQETVEVSQYSLLFNKLLDACIESNSLGMSSSVAEFMISKSIPIDFSFLRRLITSLGRSCLWLKARAHYKSALSLGCYPPLEGNLYRKLLLIPSYLSEIEMLLAIEIFLVSNASSIQSPGTSTQMLQIVLKRSEENKSRSKDDYQAAVERLITAARISDPKLFIKHMTVNVNKEQVYSLEQCSALKWLKENMKWAGKVWLFNNH

Target ORF information:

RefSeq Version	NM_001252441.1
Organism	Bos taurus(cattle)
Definition	Bos taurus testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252441.1

ORF Insert Sequence:

ATGCCGCGGG CTCCACCCTC GGGCCCCACG ACGGCCCCGG GGCGTGAAGA CGACGCTCGA 
GGCCTGAGGA AGAGAAGCCG GCGGCCAGGG CTAGGCGAGG CAGGAGGCTG CGGCCCTGAG 
GCGGAGGGGC TCGAGGAGAG CAGGCAAAAG AGGAGGATGG TGGCTCGGGC GTCTGGGAGA 
GAAGAGGTGG AAAGTGACAA GTCAGCCAAG GAAAAAAGAA AAGTTACTGA AGCCTCAAGT 
GATGATCCAC AGCCAGGGAT TGACCTGGTA AGAAAGGAAT CATTAACCAG TTCGGAATCT 
TTCCCAACAG TGGAATGCAG TGAATTTCAA AGTATGGCTT TCTTGCAGTC TTTGGGTAAG 
GAAAGGTTGG TAGAAGGTAT TAAAAGAAGA ATTCGAATTA AAAAATGTAA AAGCTTAGAA 
AGCCCAGCTC TCAAAATGAC TGAAAATAAG GCTACACAAA ACAGTAAGGT TGAATTCCAA 
GATGAACTGT ACAAGAATAC TCTGAAATAT TCTTGTAACA GCTTGTCACC TGGAGTAGAA 
AATAATTCTG TTTTAAAATT ACATGATTGC AGTTGTCTCT CCCATTCCAA GGATTGTAAT 
GATGAAAATA ACCTTGCATA TAAACCTGAT GGAGGATGTA TGCATGTACC AGAAAATTCC 
TCAAAGTCAA AGAAAGAAAA CCCTAGGAGT CTCATAGATA AGACTGATCC AAGCAATATT 
CCTCAGCTTT TACAAACTGA AGAAAACGTA ATGCGAGTAA GTCAGTTATT GCTTGAAGAA 
AATGATTCGT ACCTAAGTAA AAATAATGGC TTATTTTCCT GCCTTCAAAG TGAAAAAACT 
AAACATTCAA TAGAGGAGAG CAGTATTGGG AGAAAATCCA GAAAAAGGAT GAAGGTGTCT 
GAAAAAGGAA ATGGAATGGT TATTGAGATG AAGTTTTCTA ATATGTGTAA CAAATCTGAG 
CTGATGTTAC AAGGAAACCA AACAGGTGCT GATGGTAAAG AAACAGAGAT TTTAGAAGCT 
AAAAAAAGTT CTTTGAAAGT TTTGAGAAAA GTAAATAATA ATACGCTGTC ACCAATGGAT 
CCTTTATTAA GTCTTCCAGA AACAGGGAAA AAAACAAGTC CTGAACATTG TGCAAATGCT 
GTGTTTCAGA AAGCCCTTGA GCAACTTTCA AAAGAAGAAA CAAAGAATGT TTCACAGCCT 
TTAGGATGTA CCAGCATGGA TCCACCAGAA GATTATTTTA AGTCAATGAA AAACTCATTA 
GTGAAGTCAC TTAGTGATTG CTTTCCTATT GAAAAGAGGT CTTCTAGGGA AAGCTTGAAG 
AATGAAGCTG AAGAATCTAA ATATAGCTGT CAAAGAACAA TACCAATGAC TGGCAAAAGA 
ACTTGGCCTT GTTATTCATG TGCTAGAATA TCTGCCCAGT GTTGGAAAAA AGCTTCCTTG 
CCACAAAGTT CTAGGCAGGA TGATTTTCTT AAACATCAAA TGAATCAAAC TCACTTAACT 
GACTCCAAAT TAATGCTACA AAGTTCTGTA ACAGAAACAA ACAGTGCATC TTCAAGTATA 
GAAAAATTGG ATTCTAATTT AAATTGTTTA CCTTCAGTCT CTACAGTGGA ACCTACTTCA 
GTGGTTATAA AGGAACCTAT AGTCAATGAT GATGAAAAAA TGAAGTCAGA GGAACTAAGC 
AGAAGTGCAT CAGAGGTAGT TTCTAATACT ACTGAAGATA CTTCATTGAC TAATATGACT 
CACAACTTAA CAGGAAGTAA AAAAAAAGAT AGAGGGAATT TAACAAAACT GAATTTGACG 
GTGGCTTCCC AGGATGGTCA GGAAGCAAAT AACTCTACAA GCAAAACTGT TCATCGAAAA 
GCATGCATTA CTAAGCAAGC ACTGGTTGTT CCAGACCTAG TTAAGATACT GAACACAGGA 
CGGCTGACTA ATTTTAAAAT TCCTTTACTT AAGAACAAAA CAGAAAAAAG AAAAGAAATT 
AATGCCAAGT CATCAGAGAG AGAAGTATAT AGTCCCCTAG AACTTCTTGA CAGTGTATCG 
GGAGCAGAGG TAAGGCAGAG TAGGACTAAA GAAAATGCTG TCACAGTAAC TTCAGGACCT 
CAATCTTTGA GTATACAACG TAGTGTAATT CCAGTGCAAG CTAGTTCTGA CTCATTCTGC 
AGTAAGAATT CCTGTATTAT TGCTCCGAGC TTTTTAAAGC AAGGTAATAA TAAACAACCA 
TCTAACCACA TCTCTGCATC AGGCCGTATT ATTTCTAATA ATGCAGCTGG TTCTCTCACA 
GTTGAAAATA ATACATTTTC TTGTGATCCT GGATGTATAG AGAAAAATCC CACCTTCTAT 
TCCAATGAAC AAGAACCATT CAAAGCAGTT TCCTCTGAAG TTAGTGGTAG AAAAATGACT 
GAGAACTTTT CAGAAATTAA AGTGGGGTTT CCAGATATTC TGAAAGCATA TGAAGATGAT 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAACTCTCAT TTCCTTCAGA GGTTCCCATG ATAAGCCAGG AGCCCAATGT TGCTGAAGAG 
CATCAATCGA CAGACTCCAA GCACATGGAA CTTCCAGACA AAAAAGAACC AAGCAATGCC 
TTGAGGGAAC TGCCTGTACC GGATCCTGGA TCAATGAAGT CAGAGATCTG TGCTTCCTTG 
TCAGCAGCAA GTGAGATAAA ACATGATTCC AAAGATGCAA ACATTTCCTT AGGAGAAGTT 
ACTCATGAAA CTTCTTCAAA TGAAAAACCG AGAGGCCTAA GTGAACAGAC AAAAAGTTCA 
GACTTGGATG AAAAGTGTAG ATTTTCAGAC AAGGTAGCTA TTCGAGAAGA AAAAGAAACT 
ATTTCTGAAG TTTGCAGAAG GGATTCTAAA AATACAGAGA TTATGGTTGG TGAATGTCAC 
TTAGCAGCAC TGGTCTCCAA ACCTCTGTGC TTACCAGTGC CCTTGCCGCC TTTGAATCTT 
AATGCTCATC AGGAAGATAC ATTACTGAAT CCACAGATGA ATGATTTCAG ATTACCAGGG 
AAACATTCTG TCCTAAAGCT GCAGAATCCT GAAATTTGTG AAATATTTAA GAGGGAAAAA 
AATGTAGGGG TGTTCCAGAA GCCACTAGGG TTGATGATAC CCCACAGATA TTGCAAATTT 
CATTTTAATA CGTTACGTGG CTGTGAGCGA TCACAGTGCA AGTTTGGTCA CGTGCCTGAG 
CAAGGGGATG AAAAGGTTTG TATGGATGTG TTTAAGAAAT ATATAAGTAT CAATGAGCTG 
TGTTTGCTAC AACGTGCAGC AAACATGTTT ATGGAGTACT ACAGAAAGTT TCTTCCAGGT 
ATACACTTTG ATTTACAAGT ATTAAATGAC CTTCTAAGTT CTTTACTCAA ACACTGTTTA 
TTGAAAGAAG TATTTCAGGT CATGAACCTC TGCATAATGA TTAAGATGCT GCCTGCTCTG 
AAAATATTAC TAAAAATATT TGAGTATGTG GCAACTATGA AATTAAGGAA TGCTGTACCT 
GCTTTAATTA ATATCTTTTG CAAGCTCCTT GAAGCTGGGA TGGTACTTGA CCCAGAACAC 
TTTAACTATA TTGTTAAGCT TTTATACCAA GTACAGGCTT CCAAGCAAGA AATAACTGCA 
GTTCTGGAAA TGAAATCCAG GTTACACATG AGGCAATTTA AAAAGAACTG GAAATGTGAC 
TTAGAGGCAG CCTTGAATGA AATAGAGCAT TGTAAAGAAA AAGGTGACTG GACCAAACTG 
GGAAATGTAT ACCTTAACAT AAAAATGAGC TGTGAAAAAT TTGCAGATTT CCAGAGGTTT 
TGTGCTTGTA TTGCTGAAAC ACTAACAAAA GACTGTAAAG AAGAGCGACC AGGTGTTCCA 
TTTTGTGAAT TTGCTGAAAC AGTTAGCAAA GATCTGCAGA ATAGTGAAGT AGATAAAACT 
TTGCTGGGAA GAATCGGAAT CAGTGCTATG TACTTCTATC ACAAGCTGTT GCAGTGGTCC 
AAGGGAAGGA AGGTCTTAGA TAAACTATAT GAATTAAAAA TACATTTTAC AAGTTTAAAA 
GGACTTATAG GGCCTGAGAA GTTAGCACCA AGATGTCAAA TTGTGAATAT TGCAGCAGAA 
ATTTTTCTAA AAAGTGGAAG CCTAGATGGT GCCATTTGGG TATTAAGGGA GTCTGAGTGG 
ATCATTAATA CACCAGTATG GCCTTGCGAT CGACTGGATG TGCTCAATCG GCATAATCTG 
CTCTGTACAA TCGCACACGA AATCTTAGCG AAGAGTCTTT ACAGACAGAC ATTTGAAGTT 
TTGCAAAATC TACCAGGTTT TCAAAATTCT CAAGAAACTG TGGAGGTCTC ACAGTATAGC 
CTACTTTTTA ATAAACTTCT AGATGCCTGT ATAGAAAGCA ACAGCCTCGG TATGTCATCC 
TCTGTAGCAG AATTCATGAT TTCAAAGAGC ATCCCTATTG ATTTTTCCTT TCTTAGAAGA 
TTAATTACTT CTTTAGGAAG GAGTTGTTTA TGGCTCAAAG CCAGAGCCCA CTACAAAAGT 
GCTCTTTCAC TGGGTTGCTA CCCACCACTG GAAGGAAATT TGTACCGAAA ACTTCTCCTG 
ATTCCATCCT ATTTGTCTGA AATTGAAATG CTTTTAGCTA TTGAAATCTT CCTGGTATCT 
AATGCTAGTA GTATTCAGAG TCCTGGAACT TCTACACAGA TGCTGCAGAT AGTTTTAAAA 
AGATCTGAAG AAAACAAATC TCGGAGCAAG GATGATTATC AAGCTGCAGT GGAAAGATTA 
ATTACGGCTG CTCGTATATC AGATCCAAAG CTTTTCATTA AGCACATGAC TGTCAATGTT 
AATAAGGAAC AGGTTTATAG TTTGGAACAG TGTTCTGCCC TGAAATGGTT GAAAGAGAAT 
ATGAAGTGGG CCGGAAAGGT TTGGCTTTTC AATAACCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates.
PloS one6(11)e26950(2011)
Baillet A,Le Bouffant R,Volff JN,Luangpraseuth A,Poumerol E,Th?pot D,Pailhoux E,Livera G,Cotinot C,Mandon-P?pin B

The genome sequence of taurine cattle: a window to ruminant biology and evolution.
Science (New York, N.Y.)324(5926)522-8(2009 Apr)
,Elsik CG,Tellam RL,Worley KC,Gibbs RA,Muzny DM,Weinstock GM,Adelson DL,Eichler EE,Elnitski L,Guig? R,Hamernik DL,Kappes SM,Lewin HA,Lynn DJ,Nicholas FW,Reymond A,Rijnkels M,Skow LC,Zdobnov EM,Schook L,Womack J,Alioto T,Antonarakis SE,Astashyn A,Chapple CE,Chen HC,Chrast J,C?mara F,Ermolaeva O,Henrichsen CN,Hlavina W,Kapustin Y,Kiryutin B,Kitts P,Kokocinski F,Landrum M,Maglott D,Pruitt K,Sapojnikov V,Searle SM,Solovyev V,Souvorov A,Ucla C,Wyss C,Anzola JM,Gerlach D,Elhaik E,Graur D,Reese JT,Edgar RC,McEwan JC,Payne GM,Raison JM,Junier T,Kriventseva EV,Eyras E,Plass M,Donthu R,Larkin DM,Reecy J,Yang MQ,Chen L,Cheng Z,Chitko-McKown CG,Liu GE,Matukumalli LK,Song J,Zhu B,Bradley DG,Brinkman FS,Lau LP,Whiteside MD,Walker A,Wheeler TT,Casey T,German JB,Lemay DG,Maqbool NJ,Molenaar AJ,Seo S,Stothard P,Baldwin CL,Baxter R,Brinkmeyer-Langford CL,Brown WC,Childers CP,Connelley T,Ellis SA,Fritz K,Glass EJ,Herzig CT,Iivanainen A,Lahmers KK,Bennett AK,Dickens CM,Gilbert JG,Hagen DE,Salih H,Aerts J,Caetano AR,Dalrymple B,Garcia JF,Gill CA,Hiendleder SG,Memili E,Spurlock D,Williams JL,Alexander L,Brownstein MJ,Guan L,Holt RA,Jones SJ,Marra MA,Moore R,Moore SS,Roberts A,Taniguchi M,Waterman RC,Chacko J,Chandrabose MM,Cree A,Dao MD,Dinh HH,Gabisi RA,Hines S,Hume J,Jhangiani SN,Joshi V,Kovar CL,Lewis LR,Liu YS,Lopez J,Morgan MB,Nguyen NB,Okwuonu GO,Ruiz SJ,Santibanez J,Wright RA,Buhay C,Ding Y,Dugan-Rocha S,Herdandez J,Holder M,Sabo A,Egan A,Goodell J,Wilczek-Boney K,Fowler GR,Hitchens ME,Lozado RJ,Moen C,Steffen D,Warren JT,Zhang J,Chiu R,Schein JE,Durbin KJ,Havlak P,Jiang H,Liu Y,Qin X,Ren Y,Shen Y,Song H,Bell SN,Davis C,Johnson AJ,Lee S,Nazareth LV,Patel BM,Pu LL,Vattathil S,Williams RL Jr,Curry S,Hamilton C,Sodergren E,Wheeler DA,Barris W,Bennett GL,Eggen A,Green RD,Harhay GP,Hobbs M,Jann O,Keele JW,Kent MP,Lien S,McKay SD,McWilliam S,Ratnakumar A,Schnabel RD,Smith T,Snelling WM,Sonstegard TS,Stone RT,Sugimoto Y,Takasuga A,Taylor JF,Van Tassell CP,Macneil MD,Abatepaulo AR,Abbey CA,Ahola V,Almeida IG,Amadio AF,Anatriello E,Bahadue SM,Biase FH,Boldt CR,Carroll JA,Carvalho WA,Cervelatti EP,Chacko E,Chapin JE,Cheng Y,Choi J,Colley AJ,de Campos TA,De Donato M,Santos IK,de Oliveira CJ,Deobald H,Devinoy E,Donohue KE,Dovc P,Eberlein A,Fitzsimmons CJ,Franzin AM,Garcia GR,Genini S,Gladney CJ,Grant JR,Greaser ML,Green JA,Hadsell DL,Hakimov HA,Halgren R,Harrow JL,Hart EA,Hastings N,Hernandez M,Hu ZL,Ingham A,Iso-Touru T,Jamis C,Jensen K,Kapetis D,Kerr T,Khalil SS,Khatib H,Kolbehdari D,Kumar CG,Kumar D,Leach R,Lee JC,Li C,Logan KM,Malinverni R,Marques E,Martin WF,Martins NF,Maruyama SR,Mazza R,McLean KL,Medrano JF,Moreno BT,Mor? DD,Muntean CT,Nandakumar HP,Nogueira MF,Olsaker I,Pant SD,Panzitta F,Pastor RC,Poli MA,Poslusny N,Rachagani S,Ranganathan S,Razpet A,Riggs PK,Rincon G,Rodriguez-Osorio N,Rodriguez-Zas SL,Romero NE,Rosenwald A,Sando L,Schmutz SM,Shen L,Sherman L,Southey BR,Lutzow YS,Sweedler JV,Tammen I,Telugu BP,Urbanski JM,Utsunomiya YT,Verschoor CP,Waardenberg AJ,Wang Z,Ward R,Weikard R,Welsh TH Jr,White SN,Wilming LG,Wunderlich KR,Yang J,Zhao FQ

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TOPAZ1 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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