TRPM4 cDNA ORF clone, Bos taurus(cattle)

The following TRPM4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225693 XM_024979249.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa225690 XM_024979246.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa225691 XM_024979247.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa225692 XM_024979248.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa14297 XM_002701936.5
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa14297 XM_002695205.6
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa59313 XM_010823820.2
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa59313 XM_010815213.3
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa14297 XM_002695205.5 Bos taurus transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa59313 XM_010815213.2 Bos taurus transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225693
    Clone ID Related Accession (Same CDS sequence) XM_024979249.1
    Accession Version XM_024979249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACGCCC TCCTGAACGA CCGACCGGAG TTCGTGCGCT TGCTTATCTC CCATGGCCTC 
    AGCCTGGGCC ATTTCCTGAC TCCAGTGCGC CTGGCCCAGC TCTACAACGC AGCGCCCCCC
    AACTCACTTA TCCACAACCT TCTGGACCAA GTGTCACACG GTGCAAGCAC CAAAGCCCCA
    GCCCTTAAAC CCTCTGAGGA GCCACAGTTT CCTAAGGTGG GGCAGGTGCT GCGCATGCTG
    CTGGGGAAGA CGTGCGCGCC GACGTTCCCC GCCGGGGGCA CCCGCCGGAG CGACAACAGC
    AGAGAGAATT CGTTTATGCT GTCAGACAAG GCTGCCTCTG GACTGATGGA AGCTGTCCCC
    GGGCAGGCCC CCTGGAGCGA CCTGCTCCTC TGGGCACTGC TGCTCAACAG GGCTCAGATG
    GCCTTGTACT TCTGGGAGAT GGGTTCCAAT TCTGTGGCCT CAGCTCTTGG GGCCTCTTTG
    TTGCTGCGAG TGTTGGGGCG CCTGGAGACT GAGGCCGAGG AGGCGGCGCG GAGGAAGGAG
    CTGGCGGCCA AGTTCGAGGG GCTGGGTGTT GACCTCTTTG GAGAATGCTA CCGCAGCAGC
    GAGGAACGGT CCGCCCGCCT GCTCCTTCGG CGCTGCCCAC TCTGGGGTGA TGCCACTTGC
    CTGCAGCTCG CCACACAGGC GGATGCCCGT GCCTTCTTTG CCCAGGATGG GGTACAGTCT
    CTGCTGACAC AGAAGTGGTG GGGGGAGATG GACAGCACCA CGCCCATCTG GGCCCTGGTT
    CTTGCCTTCT TTTGCCCCCC ACTCATCTAC ACCGACCTCA TCACCTTCAG GAAGTCAGAT
    GAGGAGCCCA CGCAGAAGGA CCCGATGCTT GACGTGGATG GCGACATCAA TGGGGAAGGG
    CCTGTCGAGG CTACAGAGCC CCCTGAGAAG ATGCCCTTGG GGGTACTGAG GCCGCCCAGA
    CACAGGGACT GCTGTGGGGG GTGCCTGCCG CGCCTGCGCC GCTGGCCGCA GTTCTGGGGG
    GCGCCGGTGA CGGCCTTCAT GGGCAACGTG GTCAGCTACC TCCTCTTCCT GCTGCTGTTT
    GCGCGCGTGC TGCTCATCGA CTTCGAGCCG GAGAGGCCCA GCGTGCTGGA GCTGCTGCTC
    TACTTCTGGG CCTTCACCCT GCTCTGCGAG GAGTTCCGCC AGGGCCTGGG CAGCGGCCTG
    GGCAGCCTGG CCATGGGGGG ACCCGGGACA GACTCCCACC GGGCCCCACT GCGCCGTCGT
    CTGCAACTCT ACCTCTCTGA CGCCTGGAAC CAGTGCGACC TGGTGGCCCT CGCCTGCTTC
    GTTCTGGGCG TGGGCTGCAG GCTGACCCCG GGCCTGTATG ACCTGGGCCG CACTGTCCTC
    TGCCTTGACT TCATGATCTT CACGCTGAGG CTGCTGCACA TCTTCACAGT CAACAAACAG
    CTGGGGCCCA AGATCGTCAT CGTGAGCAAG ATGGTGAAGG ATGTGTTCTT CTTCCTCTTC
    TTCCTCGGCG TGTGGCTGGT TGCCTACGGG GTGGCCACTG AGGGGCTCCT TAGGCCCCAG
    GACCGTAGCC TCCCGAATAT CCTGCGCCGT GTCTTCTACC GGCCCTACCT GCAGATCTTT
    GGGCAGATCC CTCAGGGGGA GATGGACGTG GCCCTCATGG AGCACGGCAA CTGTTCACTG
    GGGCAGGGCT CATGGGGGCA CCCCGAAGGG AACCTCTCTG GCAAATGCGT CTCGCTTTAT
    GCCAACTGGC TGGTGGTGCT CCTCCTTGTT GTCTTCCTGC TGGTGGCCAA CATCCTGCTG
    CTCAATCTGC TCATTGCGAT GTTCAGCCAT ACCTTCGGCA AAGTGCAGGG CAACAGCGAC
    CTCTACTGGA AGGCGCAGCG CTACAGCCTC ATCCGGGAAT TTCACTCTCG GCCCGCACTG
    GCCCCGCCTC TCATTTTCAT ATCGCACGTG CGCTCCCTCA TCCATCGATT TCGCAGGCGC
    CGGGCGCGCT TGCCACCATC CCCAGACGTC GAGCATTTTC GGGTCCACCT CTCGAAGGAA
    GCGGAGCGGA GGCTGTTGAC GTGGGAGTCA GTGCAAAAGG AGAATTTCTT GCTGGCGCGT
    GCTCGGGACA AACGGGAGAG CAACTCTGAA CGTCTGAAGC GCACGTCGCA GAAGGTGGAC
    ACAGCACTGA AGCAGCTGAC ACAGATCCGC GAGTACGAGC AGCGTCTGAA AGGGCTGGAA
    CTGGAGGTCC AGCACTGTAC CCGCGTCCTG GGCTGGGTAG CTGAGGCCCT GAGCCGTTCT
    GCATTGCTTC CCCCAGGGGG ACCACCCCCC CCAATCCCAC CTGGGCCCAA AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835017.1
    CDS128..2464
    Translation

    Target ORF information:

    RefSeq Version XM_024979249.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACGCCC TCCTGAACGA CCGACCGGAG TTCGTGCGCT TGCTTATCTC CCATGGCCTC 
    AGCCTGGGCC ATTTCCTGAC TCCAGTGCGC CTGGCCCAGC TCTACAACGC AGCGCCCCCC
    AACTCACTTA TCCACAACCT TCTGGACCAA GTGTCACACG GTGCAAGCAC CAAAGCCCCA
    GCCCTTAAAC CCTCTGAGGA GCCACAGTTT CCTAAGGTGG GGCAGGTGCT GCGCATGCTG
    CTGGGGAAGA CGTGCGCGCC GACGTTCCCC GCCGGGGGCA CCCGCCGGAG CGACAACAGC
    AGAGAGAATT CGTTTATGCT GTCAGACAAG GCTGCCTCTG GACTGATGGA AGCTGTCCCC
    GGGCAGGCCC CCTGGAGCGA CCTGCTCCTC TGGGCACTGC TGCTCAACAG GGCTCAGATG
    GCCTTGTACT TCTGGGAGAT GGGTTCCAAT TCTGTGGCCT CAGCTCTTGG GGCCTCTTTG
    TTGCTGCGAG TGTTGGGGCG CCTGGAGACT GAGGCCGAGG AGGCGGCGCG GAGGAAGGAG
    CTGGCGGCCA AGTTCGAGGG GCTGGGTGTT GACCTCTTTG GAGAATGCTA CCGCAGCAGC
    GAGGAACGGT CCGCCCGCCT GCTCCTTCGG CGCTGCCCAC TCTGGGGTGA TGCCACTTGC
    CTGCAGCTCG CCACACAGGC GGATGCCCGT GCCTTCTTTG CCCAGGATGG GGTACAGTCT
    CTGCTGACAC AGAAGTGGTG GGGGGAGATG GACAGCACCA CGCCCATCTG GGCCCTGGTT
    CTTGCCTTCT TTTGCCCCCC ACTCATCTAC ACCGACCTCA TCACCTTCAG GAAGTCAGAT
    GAGGAGCCCA CGCAGAAGGA CCCGATGCTT GACGTGGATG GCGACATCAA TGGGGAAGGG
    CCTGTCGAGG CTACAGAGCC CCCTGAGAAG ATGCCCTTGG GGGTACTGAG GCCGCCCAGA
    CACAGGGACT GCTGTGGGGG GTGCCTGCCG CGCCTGCGCC GCTGGCCGCA GTTCTGGGGG
    GCGCCGGTGA CGGCCTTCAT GGGCAACGTG GTCAGCTACC TCCTCTTCCT GCTGCTGTTT
    GCGCGCGTGC TGCTCATCGA CTTCGAGCCG GAGAGGCCCA GCGTGCTGGA GCTGCTGCTC
    TACTTCTGGG CCTTCACCCT GCTCTGCGAG GAGTTCCGCC AGGGCCTGGG CAGCGGCCTG
    GGCAGCCTGG CCATGGGGGG ACCCGGGACA GACTCCCACC GGGCCCCACT GCGCCGTCGT
    CTGCAACTCT ACCTCTCTGA CGCCTGGAAC CAGTGCGACC TGGTGGCCCT CGCCTGCTTC
    GTTCTGGGCG TGGGCTGCAG GCTGACCCCG GGCCTGTATG ACCTGGGCCG CACTGTCCTC
    TGCCTTGACT TCATGATCTT CACGCTGAGG CTGCTGCACA TCTTCACAGT CAACAAACAG
    CTGGGGCCCA AGATCGTCAT CGTGAGCAAG ATGGTGAAGG ATGTGTTCTT CTTCCTCTTC
    TTCCTCGGCG TGTGGCTGGT TGCCTACGGG GTGGCCACTG AGGGGCTCCT TAGGCCCCAG
    GACCGTAGCC TCCCGAATAT CCTGCGCCGT GTCTTCTACC GGCCCTACCT GCAGATCTTT
    GGGCAGATCC CTCAGGGGGA GATGGACGTG GCCCTCATGG AGCACGGCAA CTGTTCACTG
    GGGCAGGGCT CATGGGGGCA CCCCGAAGGG AACCTCTCTG GCAAATGCGT CTCGCTTTAT
    GCCAACTGGC TGGTGGTGCT CCTCCTTGTT GTCTTCCTGC TGGTGGCCAA CATCCTGCTG
    CTCAATCTGC TCATTGCGAT GTTCAGCCAT ACCTTCGGCA AAGTGCAGGG CAACAGCGAC
    CTCTACTGGA AGGCGCAGCG CTACAGCCTC ATCCGGGAAT TTCACTCTCG GCCCGCACTG
    GCCCCGCCTC TCATTTTCAT ATCGCACGTG CGCTCCCTCA TCCATCGATT TCGCAGGCGC
    CGGGCGCGCT TGCCACCATC CCCAGACGTC GAGCATTTTC GGGTCCACCT CTCGAAGGAA
    GCGGAGCGGA GGCTGTTGAC GTGGGAGTCA GTGCAAAAGG AGAATTTCTT GCTGGCGCGT
    GCTCGGGACA AACGGGAGAG CAACTCTGAA CGTCTGAAGC GCACGTCGCA GAAGGTGGAC
    ACAGCACTGA AGCAGCTGAC ACAGATCCGC GAGTACGAGC AGCGTCTGAA AGGGCTGGAA
    CTGGAGGTCC AGCACTGTAC CCGCGTCCTG GGCTGGGTAG CTGAGGCCCT GAGCCGTTCT
    GCATTGCTTC CCCCAGGGGG ACCACCCCCC CCAATCCCAC CTGGGCCCAA AGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225690
    Clone ID Related Accession (Same CDS sequence) XM_024979246.1
    Accession Version XM_024979246.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3645bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGTTTC TGTCCGGACC TATTCCTGCT AGTGTAGGCT TCCAGAGCTG GATTCCCAAG 
    ATCTTCAAGA AGAAGACGTG CACGACTTTC GTCGCTGACC CCACAGATTC GGGGGGGAGC
    TTGTGCCAGT GTGGGCATCC TCGGGGTGCC CACCCATCCG TGGCTGTGGA GGATGCATTC
    GGGGCAGCCG TGGTGACCGT ATGGGACAGT GACTTGCACA CCACGGAGAA GCCCACAGAT
    GCCTTCGGGG ACCTGGACTT CCTGGGCGCC GGCCGCAAGA TCAGCAATTT CCTCCGGCTC
    TCGGACCGCA CGGATCCAGC TACGGTTTAT ACTCTGGTCA CCAAAGTGTG GGGCTTCCGG
    GCCCCGAACC TGGTGGTGTC AGTGCTGGGG GGGTCAGGGG GCCCCATCCT CCAGACCTGG
    CTGCAGGACC TGCTGCGACG CGGGCTGGTG CGGGCTGCCC AGAGCACAGG GGCCTGGATT
    GTCACTGGAG GGCTGCACAA AGGCATCGGC CGGCACGTCG GTGCGGCTGT GCGGGACCAC
    CAGACGGCCA GCACTGGGGG CAGCAAAGTG GTGGCCATGG GCGTGGCCCC CTGGGGCGTG
    GTGCGGAATA GAGACACTCT CACCAACCCC AAGGGCTCGT TCCCCGCAAG GTACCGGTGG
    CGCGGAGACC CAGAGGATGG GGTCCAGTTC CCTCTTGACT ACAACTACTC GGCCTTCTTC
    CTGGTGGATG ACGGCACACA CGGCCGCCTG GGCGGCGAGA ACCGCTTCCG CTTGGGCTTC
    GAGTCCTACC TGGCCCAACA GAAGACGGGC GTGGGAGGGA CTGGCATTGA CATCCCTGTC
    CTCCTCCTCC TGATTGATGG TGATGAGAAG ATGTTGAAGC AGATAGAGGA TGCCACCCAG
    GCTCAGCTCC CCTGCCTCCT GGTGGCTGGA TCTGGGGGAG CTGCGGACTG CCTGGCAGAG
    ATCCTGGAGG ACACTCTGGC TCCAGGGAGT GGCGGGGGCA GGCGACGTGA ACCCCGAGAT
    CTGATTAAGC GTTTCTTCCC CAAAGGGGAC CCTGAGGTCC TGCAGGCCCA GGTGGAGAGG
    ATCATGACTC GGAAGGAGCT GCTGACGGTC TATTCAACTG ATGACGGCCC TGAGGAATTT
    GAGACCATCG TCTTAAGGGC CCTTGTCAAG GCCTGTGGGA GCTCTGAAGC CTCAGCTTAC
    CTGGACGAGT TGCGTTTGGC TGTGGCTTGG AACCGTGTGG ACATTGCCCA GAGTGAACTC
    TTCCGGGGGG ACATCCAATG GCGGTCCTTC CACCTGGAGG CCTCCCTCAT GGACGCCCTC
    CTGAACGACC GACCGGAGTT CGTGCGCTTG CTTATCTCCC ATGGCCTCAG CCTGGGCCAT
    TTCCTGACTC CAGTGCGCCT GGCCCAGCTC TACAACGCAG CGCCCCCCAA CTCACTTATC
    CACAACCTTC TGGACCAAGT GTCACACGGT GCAAGCACCA AAGCCCCAGC CCTTAAACCC
    TCTGAGGAGC CACAGTTTCC TAAGGTGGGG CAGGTGCTGC GCATGCTGCT GGGGAAGACG
    TGCGCGCCGA CGTTCCCCGC CGGGGGCACC CGCCGGAGCG ACAACAGCAG AGAGAATTCG
    TTTATGCTGT CAGACAAGGC TGCCTCTGGA CTGATGGAAG CTGTCCCCGG GCAGGCCCCC
    TGGAGCGACC TGCTCCTCTG GGCACTGCTG CTCAACAGGG CTCAGATGGC CTTGTACTTC
    TGGGAGATGG GTTCCAATTC TGTGGCCTCA GCTCTTGGGG CCTCTTTGTT GCTGCGAGTG
    TTGGGGCGCC TGGAGACTGA GGCCGAGGAG GCGGCGCGGA GGAAGGAGCT GGCGGCCAAG
    TTCGAGGGGC TGGGTGTTGA CCTCTTTGGA GAATGCTACC GCAGCAGCGA GGAACGGTCC
    GCCCGCCTGC TCCTTCGGCG CTGCCCACTC TGGGGTGATG CCACTTGCCT GCAGCTCGCC
    ACACAGGCGG ATGCCCGTGC CTTCTTTGCC CAGGATGGGG TACAGTCTCT GCTGACACAG
    AAGTGGTGGG GGGAGATGGA CAGCACCACG CCCATCTGGG CCCTGGTTCT TGCCTTCTTT
    TGCCCCCCAC TCATCTACAC CGACCTCATC ACCTTCAGGA AGTCAGATGA GGAGCCCACG
    CAGAAGGACC CGATGCTTGA CGTGGATGGC GACATCAATG GGGAAGGGCC TGTCGAGGCT
    ACAGAGCCCC CTGAGAAGAT GCCCTTGGGG GTACTGAGGC CGCCCAGACA CAGGGACTGC
    TGTGGGGGGT GCCTGCCGCG CCTGCGCCGC TGGCCGCAGT TCTGGGGGGC GCCGGTGACG
    GCCTTCATGG GCAACGTGGT CAGCTACCTC CTCTTCCTGC TGCTGTTTGC GCGCGTGCTG
    CTCATCGACT TCGAGCCGGA GAGGCCCAGC GTGCTGGAGC TGCTGCTCTA CTTCTGGGCC
    TTCACCCTGC TCTGCGAGGA GTTCCGCCAG GGCCTGGGCA GCGGCCTGGG CAGCCTGGCC
    ATGGGGGGAC CCGGGACAGA CTCCCACCGG GCCCCACTGC GCCGTCGTCT GCAACTCTAC
    CTCTCTGACG CCTGGAACCA GTGCGACCTG GTGGCCCTCG CCTGCTTCGT TCTGGGCGTG
    GGCTGCAGGC TGACCCCGGG CCTGTATGAC CTGGGCCGCA CTGTCCTCTG CCTTGACTTC
    ATGATCTTCA CGCTGAGGCT GCTGCACATC TTCACAGTCA ACAAACAGCT GGGGCCCAAG
    ATCGTCATCG TGAGCAAGAT GGTGAAGGAT GTGTTCTTCT TCCTCTTCTT CCTCGGCGTG
    TGGCTGGTTG CCTACGGGGT GGCCACTGAG GGGCTCCTTA GGCCCCAGGA CCGTAGCCTC
    CCGAATATCC TGCGCCGTGT CTTCTACCGG CCCTACCTGC AGATCTTTGG GCAGATCCCT
    CAGGGGGAGA TGGACGTGGC CCTCATGGAG CACGGCAACT GTTCACTGGG GCAGGGCTCA
    TGGGGGCACC CCGAAGGGAA CCTCTCTGGC AAATGCGTCT CGCTTTATGC CAACTGGCTG
    GTGGTGCTCC TCCTTGTTGT CTTCCTGCTG GTGGCCAACA TCCTGCTGCT CAATCTGCTC
    ATTGCGATGT TCAGCCATAC CTTCGGCAAA GTGCAGGGCA ACAGCGACCT CTACTGGAAG
    GCGCAGCGCT ACAGCCTCAT CCGGGAATTT CACTCTCGGC CCGCACTGGC CCCGCCTCTC
    ATTTTCATAT CGCACGTGCG CTCCCTCATC CATCGATTTC GCAGGCGCCG GGCGCGCTTG
    CCACCATCCC CAGACGTCGA GCATTTTCGG GTCCACCTCT CGAAGGAAGC GGAGCGGAGG
    CTGTTGACGT GGGAGTCAGT GCAAAAGGAG AATTTCTTGC TGGCGCGTGC TCGGGACAAA
    CGGGAGAGCA ACTCTGAACG TCTGAAGCGC ACGTCGCAGA AGGTGGACAC AGCACTGAAG
    CAGCTGACAC AGATCCGCGA GTACGAGCAG CGTCTGAAAG GGCTGGAACT GGAGGTCCAG
    CACTGTACCC GCGTCCTGGG CTGGGTAGCT GAGGCCCTGA GCCGTTCTGC ATTGCTTCCC
    CCAGGGGGAC CACCCCCCCC AATCCCACCT GGGCCCAAAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835014.1
    CDS22..3666
    Translation

    Target ORF information:

    RefSeq Version XM_024979246.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGTTTC TGTCCGGACC TATTCCTGCT AGTGTAGGCT TCCAGAGCTG GATTCCCAAG 
    ATCTTCAAGA AGAAGACGTG CACGACTTTC GTCGCTGACC CCACAGATTC GGGGGGGAGC
    TTGTGCCAGT GTGGGCATCC TCGGGGTGCC CACCCATCCG TGGCTGTGGA GGATGCATTC
    GGGGCAGCCG TGGTGACCGT ATGGGACAGT GACTTGCACA CCACGGAGAA GCCCACAGAT
    GCCTTCGGGG ACCTGGACTT CCTGGGCGCC GGCCGCAAGA TCAGCAATTT CCTCCGGCTC
    TCGGACCGCA CGGATCCAGC TACGGTTTAT ACTCTGGTCA CCAAAGTGTG GGGCTTCCGG
    GCCCCGAACC TGGTGGTGTC AGTGCTGGGG GGGTCAGGGG GCCCCATCCT CCAGACCTGG
    CTGCAGGACC TGCTGCGACG CGGGCTGGTG CGGGCTGCCC AGAGCACAGG GGCCTGGATT
    GTCACTGGAG GGCTGCACAA AGGCATCGGC CGGCACGTCG GTGCGGCTGT GCGGGACCAC
    CAGACGGCCA GCACTGGGGG CAGCAAAGTG GTGGCCATGG GCGTGGCCCC CTGGGGCGTG
    GTGCGGAATA GAGACACTCT CACCAACCCC AAGGGCTCGT TCCCCGCAAG GTACCGGTGG
    CGCGGAGACC CAGAGGATGG GGTCCAGTTC CCTCTTGACT ACAACTACTC GGCCTTCTTC
    CTGGTGGATG ACGGCACACA CGGCCGCCTG GGCGGCGAGA ACCGCTTCCG CTTGGGCTTC
    GAGTCCTACC TGGCCCAACA GAAGACGGGC GTGGGAGGGA CTGGCATTGA CATCCCTGTC
    CTCCTCCTCC TGATTGATGG TGATGAGAAG ATGTTGAAGC AGATAGAGGA TGCCACCCAG
    GCTCAGCTCC CCTGCCTCCT GGTGGCTGGA TCTGGGGGAG CTGCGGACTG CCTGGCAGAG
    ATCCTGGAGG ACACTCTGGC TCCAGGGAGT GGCGGGGGCA GGCGACGTGA ACCCCGAGAT
    CTGATTAAGC GTTTCTTCCC CAAAGGGGAC CCTGAGGTCC TGCAGGCCCA GGTGGAGAGG
    ATCATGACTC GGAAGGAGCT GCTGACGGTC TATTCAACTG ATGACGGCCC TGAGGAATTT
    GAGACCATCG TCTTAAGGGC CCTTGTCAAG GCCTGTGGGA GCTCTGAAGC CTCAGCTTAC
    CTGGACGAGT TGCGTTTGGC TGTGGCTTGG AACCGTGTGG ACATTGCCCA GAGTGAACTC
    TTCCGGGGGG ACATCCAATG GCGGTCCTTC CACCTGGAGG CCTCCCTCAT GGACGCCCTC
    CTGAACGACC GACCGGAGTT CGTGCGCTTG CTTATCTCCC ATGGCCTCAG CCTGGGCCAT
    TTCCTGACTC CAGTGCGCCT GGCCCAGCTC TACAACGCAG CGCCCCCCAA CTCACTTATC
    CACAACCTTC TGGACCAAGT GTCACACGGT GCAAGCACCA AAGCCCCAGC CCTTAAACCC
    TCTGAGGAGC CACAGTTTCC TAAGGTGGGG CAGGTGCTGC GCATGCTGCT GGGGAAGACG
    TGCGCGCCGA CGTTCCCCGC CGGGGGCACC CGCCGGAGCG ACAACAGCAG AGAGAATTCG
    TTTATGCTGT CAGACAAGGC TGCCTCTGGA CTGATGGAAG CTGTCCCCGG GCAGGCCCCC
    TGGAGCGACC TGCTCCTCTG GGCACTGCTG CTCAACAGGG CTCAGATGGC CTTGTACTTC
    TGGGAGATGG GTTCCAATTC TGTGGCCTCA GCTCTTGGGG CCTCTTTGTT GCTGCGAGTG
    TTGGGGCGCC TGGAGACTGA GGCCGAGGAG GCGGCGCGGA GGAAGGAGCT GGCGGCCAAG
    TTCGAGGGGC TGGGTGTTGA CCTCTTTGGA GAATGCTACC GCAGCAGCGA GGAACGGTCC
    GCCCGCCTGC TCCTTCGGCG CTGCCCACTC TGGGGTGATG CCACTTGCCT GCAGCTCGCC
    ACACAGGCGG ATGCCCGTGC CTTCTTTGCC CAGGATGGGG TACAGTCTCT GCTGACACAG
    AAGTGGTGGG GGGAGATGGA CAGCACCACG CCCATCTGGG CCCTGGTTCT TGCCTTCTTT
    TGCCCCCCAC TCATCTACAC CGACCTCATC ACCTTCAGGA AGTCAGATGA GGAGCCCACG
    CAGAAGGACC CGATGCTTGA CGTGGATGGC GACATCAATG GGGAAGGGCC TGTCGAGGCT
    ACAGAGCCCC CTGAGAAGAT GCCCTTGGGG GTACTGAGGC CGCCCAGACA CAGGGACTGC
    TGTGGGGGGT GCCTGCCGCG CCTGCGCCGC TGGCCGCAGT TCTGGGGGGC GCCGGTGACG
    GCCTTCATGG GCAACGTGGT CAGCTACCTC CTCTTCCTGC TGCTGTTTGC GCGCGTGCTG
    CTCATCGACT TCGAGCCGGA GAGGCCCAGC GTGCTGGAGC TGCTGCTCTA CTTCTGGGCC
    TTCACCCTGC TCTGCGAGGA GTTCCGCCAG GGCCTGGGCA GCGGCCTGGG CAGCCTGGCC
    ATGGGGGGAC CCGGGACAGA CTCCCACCGG GCCCCACTGC GCCGTCGTCT GCAACTCTAC
    CTCTCTGACG CCTGGAACCA GTGCGACCTG GTGGCCCTCG CCTGCTTCGT TCTGGGCGTG
    GGCTGCAGGC TGACCCCGGG CCTGTATGAC CTGGGCCGCA CTGTCCTCTG CCTTGACTTC
    ATGATCTTCA CGCTGAGGCT GCTGCACATC TTCACAGTCA ACAAACAGCT GGGGCCCAAG
    ATCGTCATCG TGAGCAAGAT GGTGAAGGAT GTGTTCTTCT TCCTCTTCTT CCTCGGCGTG
    TGGCTGGTTG CCTACGGGGT GGCCACTGAG GGGCTCCTTA GGCCCCAGGA CCGTAGCCTC
    CCGAATATCC TGCGCCGTGT CTTCTACCGG CCCTACCTGC AGATCTTTGG GCAGATCCCT
    CAGGGGGAGA TGGACGTGGC CCTCATGGAG CACGGCAACT GTTCACTGGG GCAGGGCTCA
    TGGGGGCACC CCGAAGGGAA CCTCTCTGGC AAATGCGTCT CGCTTTATGC CAACTGGCTG
    GTGGTGCTCC TCCTTGTTGT CTTCCTGCTG GTGGCCAACA TCCTGCTGCT CAATCTGCTC
    ATTGCGATGT TCAGCCATAC CTTCGGCAAA GTGCAGGGCA ACAGCGACCT CTACTGGAAG
    GCGCAGCGCT ACAGCCTCAT CCGGGAATTT CACTCTCGGC CCGCACTGGC CCCGCCTCTC
    ATTTTCATAT CGCACGTGCG CTCCCTCATC CATCGATTTC GCAGGCGCCG GGCGCGCTTG
    CCACCATCCC CAGACGTCGA GCATTTTCGG GTCCACCTCT CGAAGGAAGC GGAGCGGAGG
    CTGTTGACGT GGGAGTCAGT GCAAAAGGAG AATTTCTTGC TGGCGCGTGC TCGGGACAAA
    CGGGAGAGCA ACTCTGAACG TCTGAAGCGC ACGTCGCAGA AGGTGGACAC AGCACTGAAG
    CAGCTGACAC AGATCCGCGA GTACGAGCAG CGTCTGAAAG GGCTGGAACT GGAGGTCCAG
    CACTGTACCC GCGTCCTGGG CTGGGTAGCT GAGGCCCTGA GCCGTTCTGC ATTGCTTCCC
    CCAGGGGGAC CACCCCCCCC AATCCCACCT GGGCCCAAAG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225691
    Clone ID Related Accession (Same CDS sequence) XM_024979247.1
    Accession Version XM_024979247.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGTTTC TGTCCGGACC TATTCCTGCT AGTGTAGGCT TCCAGAGCTG GATTCCCAAG 
    ATCTTCAAGA AGAAGACGTG CACGACTTTC GTCGCTGACC CCACAGATTC GGGGGGGAGC
    TTGTGCCAGT GTGGGCATCC TCGGGGTGCC CACCCATCCG TGGCTGTGGA GGATGCATTC
    GGGGCAGCCG TGGTGACCGT ATGGGACAGT GACTTGCACA CCACGGAGAA GCCCACAGAT
    GCCTTCGGGG ACCTGGACTT CCTGGGCGCC GGCCGCAAGA TCAGCAATTT CCTCCGGCTC
    TCGGACCGCA CGGATCCAGC TACGGTTTAT ACTCTGGTCA CCAAAGTGTG GGGCTTCCGG
    GCCCCGAACC TGGTGGTGTC AGTGCTGGGG GGGTCAGGGG GCCCCATCCT CCAGACCTGG
    CTGCAGGACC TGCTGCGACG CGGGCTGGTG CGGGCTGCCC AGAGCACAGG GGCCTGGATT
    GTCACTGGAG GGCTGCACAA AGGCATCGGC CGGCACGTCG GTGCGGCTGT GCGGGACCAC
    CAGACGGCCA GCACTGGGGG CAGCAAAGTG GTGGCCATGG GCGTGGCCCC CTGGGGCGTG
    GTGCGGAATA GAGACACTCT CACCAACCCC AAGGGCTCGT TCCCCGCAAG GTACCGGTGG
    CGCGGAGACC CAGAGGATGG GGTCCAGTTC CCTCTTGACT ACAACTACTC GGCCTTCTTC
    CTGGTGGATG ACGGCACACA CGGCCGCCTG GGCGGCGAGA ACCGCTTCCG CTTGGGCTTC
    GAGTCCTACC TGGCCCAACA GAAGACGGGC GTGGGAGGGA CTGGCATTGA CATCCCTGTC
    CTCCTCCTCC TGATTGATGG TGATGAGAAG ATGTTGAAGA TAGAGGATGC CACCCAGGCT
    CAGCTCCCCT GCCTCCTGGT GGCTGGATCT GGGGGAGCTG CGGACTGCCT GGCAGAGATC
    CTGGAGGACA CTCTGGCTCC AGGGAGTGGC GGGGGCAGGC GACGTGAACC CCGAGATCTG
    ATTAAGCGTT TCTTCCCCAA AGGGGACCCT GAGGTCCTGC AGGCCCAGGT GGAGAGGATC
    ATGACTCGGA AGGAGCTGCT GACGGTCTAT TCAACTGATG ACGGCCCTGA GGAATTTGAG
    ACCATCGTCT TAAGGGCCCT TGTCAAGGCC TGTGGGAGCT CTGAAGCCTC AGCTTACCTG
    GACGAGTTGC GTTTGGCTGT GGCTTGGAAC CGTGTGGACA TTGCCCAGAG TGAACTCTTC
    CGGGGGGACA TCCAATGGCG GTCCTTCCAC CTGGAGGCCT CCCTCATGGA CGCCCTCCTG
    AACGACCGAC CGGAGTTCGT GCGCTTGCTT ATCTCCCATG GCCTCAGCCT GGGCCATTTC
    CTGACTCCAG TGCGCCTGGC CCAGCTCTAC AACGCAGCGC CCCCCAACTC ACTTATCCAC
    AACCTTCTGG ACCAAGTGTC ACACGGTGCA AGCACCAAAG CCCCAGCCCT TAAACCCTCT
    GAGGAGCCAC AGTTTCCTAA GGTGGGGCAG GTGCTGCGCA TGCTGCTGGG GAAGACGTGC
    GCGCCGACGT TCCCCGCCGG GGGCACCCGC CGGAGCGACA ACAGCAGAGA GAATTCGTTT
    ATGCTGTCAG ACAAGGCTGC CTCTGGACTG ATGGAAGCTG TCCCCGGGCA GGCCCCCTGG
    AGCGACCTGC TCCTCTGGGC ACTGCTGCTC AACAGGGCTC AGATGGCCTT GTACTTCTGG
    GAGATGGGTT CCAATTCTGT GGCCTCAGCT CTTGGGGCCT CTTTGTTGCT GCGAGTGTTG
    GGGCGCCTGG AGACTGAGGC CGAGGAGGCG GCGCGGAGGA AGGAGCTGGC GGCCAAGTTC
    GAGGGGCTGG GTGTTGACCT CTTTGGAGAA TGCTACCGCA GCAGCGAGGA ACGGTCCGCC
    CGCCTGCTCC TTCGGCGCTG CCCACTCTGG GGTGATGCCA CTTGCCTGCA GCTCGCCACA
    CAGGCGGATG CCCGTGCCTT CTTTGCCCAG GATGGGGTAC AGTCTCTGCT GACACAGAAG
    TGGTGGGGGG AGATGGACAG CACCACGCCC ATCTGGGCCC TGGTTCTTGC CTTCTTTTGC
    CCCCCACTCA TCTACACCGA CCTCATCACC TTCAGGAAGT CAGATGAGGA GCCCACGCAG
    AAGGACCCGA TGCTTGACGT GGATGGCGAC ATCAATGGGG AAGGGCCTGT CGAGGCTACA
    GAGCCCCCTG AGAAGATGCC CTTGGGGGTA CTGAGGCCGC CCAGACACAG GGACTGCTGT
    GGGGGGTGCC TGCCGCGCCT GCGCCGCTGG CCGCAGTTCT GGGGGGCGCC GGTGACGGCC
    TTCATGGGCA ACGTGGTCAG CTACCTCCTC TTCCTGCTGC TGTTTGCGCG CGTGCTGCTC
    ATCGACTTCG AGCCGGAGAG GCCCAGCGTG CTGGAGCTGC TGCTCTACTT CTGGGCCTTC
    ACCCTGCTCT GCGAGGAGTT CCGCCAGGGC CTGGGCAGCG GCCTGGGCAG CCTGGCCATG
    GGGGGACCCG GGACAGACTC CCACCGGGCC CCACTGCGCC GTCGTCTGCA ACTCTACCTC
    TCTGACGCCT GGAACCAGTG CGACCTGGTG GCCCTCGCCT GCTTCGTTCT GGGCGTGGGC
    TGCAGGCTGA CCCCGGGCCT GTATGACCTG GGCCGCACTG TCCTCTGCCT TGACTTCATG
    ATCTTCACGC TGAGGCTGCT GCACATCTTC ACAGTCAACA AACAGCTGGG GCCCAAGATC
    GTCATCGTGA GCAAGATGGT GAAGGATGTG TTCTTCTTCC TCTTCTTCCT CGGCGTGTGG
    CTGGTTGCCT ACGGGGTGGC CACTGAGGGG CTCCTTAGGC CCCAGGACCG TAGCCTCCCG
    AATATCCTGC GCCGTGTCTT CTACCGGCCC TACCTGCAGA TCTTTGGGCA GATCCCTCAG
    GGGGAGATGG ACGTGGCCCT CATGGAGCAC GGCAACTGTT CACTGGGGCA GGGCTCATGG
    GGGCACCCCG AAGGGAACCT CTCTGGCAAA TGCGTCTCGC TTTATGCCAA CTGGCTGGTG
    GTGCTCCTCC TTGTTGTCTT CCTGCTGGTG GCCAACATCC TGCTGCTCAA TCTGCTCATT
    GCGATGTTCA GCCATACCTT CGGCAAAGTG CAGGGCAACA GCGACCTCTA CTGGAAGGCG
    CAGCGCTACA GCCTCATCCG GGAATTTCAC TCTCGGCCCG CACTGGCCCC GCCTCTCATT
    TTCATATCGC ACGTGCGCTC CCTCATCCAT CGATTTCGCA GGCGCCGGGC GCGCTTGCCA
    CCATCCCCAG ACGTCGAGCA TTTTCGGGTC CACCTCTCGA AGGAAGCGGA GCGGAGGCTG
    TTGACGTGGG AGTCAGTGCA AAAGGAGAAT TTCTTGCTGG CGCGTGCTCG GGACAAACGG
    GAGAGCAACT CTGAACGTCT GAAGCGCACG TCGCAGAAGG TGGACACAGC ACTGAAGCAG
    CTGACACAGA TCCGCGAGTA CGAGCAGCGT CTGAAAGGGC TGGAACTGGA GGTCCAGCAC
    TGTACCCGCG TCCTGGGCTG GGTAGCTGAG GCCCTGAGCC GTTCTGCATT GCTTCCCCCA
    GGGGGACCAC CCCCCCCAAT CCCACCTGGG CCCAAAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835015.1
    CDS22..3663
    Translation

    Target ORF information:

    RefSeq Version XM_024979247.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGTTTC TGTCCGGACC TATTCCTGCT AGTGTAGGCT TCCAGAGCTG GATTCCCAAG 
    ATCTTCAAGA AGAAGACGTG CACGACTTTC GTCGCTGACC CCACAGATTC GGGGGGGAGC
    TTGTGCCAGT GTGGGCATCC TCGGGGTGCC CACCCATCCG TGGCTGTGGA GGATGCATTC
    GGGGCAGCCG TGGTGACCGT ATGGGACAGT GACTTGCACA CCACGGAGAA GCCCACAGAT
    GCCTTCGGGG ACCTGGACTT CCTGGGCGCC GGCCGCAAGA TCAGCAATTT CCTCCGGCTC
    TCGGACCGCA CGGATCCAGC TACGGTTTAT ACTCTGGTCA CCAAAGTGTG GGGCTTCCGG
    GCCCCGAACC TGGTGGTGTC AGTGCTGGGG GGGTCAGGGG GCCCCATCCT CCAGACCTGG
    CTGCAGGACC TGCTGCGACG CGGGCTGGTG CGGGCTGCCC AGAGCACAGG GGCCTGGATT
    GTCACTGGAG GGCTGCACAA AGGCATCGGC CGGCACGTCG GTGCGGCTGT GCGGGACCAC
    CAGACGGCCA GCACTGGGGG CAGCAAAGTG GTGGCCATGG GCGTGGCCCC CTGGGGCGTG
    GTGCGGAATA GAGACACTCT CACCAACCCC AAGGGCTCGT TCCCCGCAAG GTACCGGTGG
    CGCGGAGACC CAGAGGATGG GGTCCAGTTC CCTCTTGACT ACAACTACTC GGCCTTCTTC
    CTGGTGGATG ACGGCACACA CGGCCGCCTG GGCGGCGAGA ACCGCTTCCG CTTGGGCTTC
    GAGTCCTACC TGGCCCAACA GAAGACGGGC GTGGGAGGGA CTGGCATTGA CATCCCTGTC
    CTCCTCCTCC TGATTGATGG TGATGAGAAG ATGTTGAAGA TAGAGGATGC CACCCAGGCT
    CAGCTCCCCT GCCTCCTGGT GGCTGGATCT GGGGGAGCTG CGGACTGCCT GGCAGAGATC
    CTGGAGGACA CTCTGGCTCC AGGGAGTGGC GGGGGCAGGC GACGTGAACC CCGAGATCTG
    ATTAAGCGTT TCTTCCCCAA AGGGGACCCT GAGGTCCTGC AGGCCCAGGT GGAGAGGATC
    ATGACTCGGA AGGAGCTGCT GACGGTCTAT TCAACTGATG ACGGCCCTGA GGAATTTGAG
    ACCATCGTCT TAAGGGCCCT TGTCAAGGCC TGTGGGAGCT CTGAAGCCTC AGCTTACCTG
    GACGAGTTGC GTTTGGCTGT GGCTTGGAAC CGTGTGGACA TTGCCCAGAG TGAACTCTTC
    CGGGGGGACA TCCAATGGCG GTCCTTCCAC CTGGAGGCCT CCCTCATGGA CGCCCTCCTG
    AACGACCGAC CGGAGTTCGT GCGCTTGCTT ATCTCCCATG GCCTCAGCCT GGGCCATTTC
    CTGACTCCAG TGCGCCTGGC CCAGCTCTAC AACGCAGCGC CCCCCAACTC ACTTATCCAC
    AACCTTCTGG ACCAAGTGTC ACACGGTGCA AGCACCAAAG CCCCAGCCCT TAAACCCTCT
    GAGGAGCCAC AGTTTCCTAA GGTGGGGCAG GTGCTGCGCA TGCTGCTGGG GAAGACGTGC
    GCGCCGACGT TCCCCGCCGG GGGCACCCGC CGGAGCGACA ACAGCAGAGA GAATTCGTTT
    ATGCTGTCAG ACAAGGCTGC CTCTGGACTG ATGGAAGCTG TCCCCGGGCA GGCCCCCTGG
    AGCGACCTGC TCCTCTGGGC ACTGCTGCTC AACAGGGCTC AGATGGCCTT GTACTTCTGG
    GAGATGGGTT CCAATTCTGT GGCCTCAGCT CTTGGGGCCT CTTTGTTGCT GCGAGTGTTG
    GGGCGCCTGG AGACTGAGGC CGAGGAGGCG GCGCGGAGGA AGGAGCTGGC GGCCAAGTTC
    GAGGGGCTGG GTGTTGACCT CTTTGGAGAA TGCTACCGCA GCAGCGAGGA ACGGTCCGCC
    CGCCTGCTCC TTCGGCGCTG CCCACTCTGG GGTGATGCCA CTTGCCTGCA GCTCGCCACA
    CAGGCGGATG CCCGTGCCTT CTTTGCCCAG GATGGGGTAC AGTCTCTGCT GACACAGAAG
    TGGTGGGGGG AGATGGACAG CACCACGCCC ATCTGGGCCC TGGTTCTTGC CTTCTTTTGC
    CCCCCACTCA TCTACACCGA CCTCATCACC TTCAGGAAGT CAGATGAGGA GCCCACGCAG
    AAGGACCCGA TGCTTGACGT GGATGGCGAC ATCAATGGGG AAGGGCCTGT CGAGGCTACA
    GAGCCCCCTG AGAAGATGCC CTTGGGGGTA CTGAGGCCGC CCAGACACAG GGACTGCTGT
    GGGGGGTGCC TGCCGCGCCT GCGCCGCTGG CCGCAGTTCT GGGGGGCGCC GGTGACGGCC
    TTCATGGGCA ACGTGGTCAG CTACCTCCTC TTCCTGCTGC TGTTTGCGCG CGTGCTGCTC
    ATCGACTTCG AGCCGGAGAG GCCCAGCGTG CTGGAGCTGC TGCTCTACTT CTGGGCCTTC
    ACCCTGCTCT GCGAGGAGTT CCGCCAGGGC CTGGGCAGCG GCCTGGGCAG CCTGGCCATG
    GGGGGACCCG GGACAGACTC CCACCGGGCC CCACTGCGCC GTCGTCTGCA ACTCTACCTC
    TCTGACGCCT GGAACCAGTG CGACCTGGTG GCCCTCGCCT GCTTCGTTCT GGGCGTGGGC
    TGCAGGCTGA CCCCGGGCCT GTATGACCTG GGCCGCACTG TCCTCTGCCT TGACTTCATG
    ATCTTCACGC TGAGGCTGCT GCACATCTTC ACAGTCAACA AACAGCTGGG GCCCAAGATC
    GTCATCGTGA GCAAGATGGT GAAGGATGTG TTCTTCTTCC TCTTCTTCCT CGGCGTGTGG
    CTGGTTGCCT ACGGGGTGGC CACTGAGGGG CTCCTTAGGC CCCAGGACCG TAGCCTCCCG
    AATATCCTGC GCCGTGTCTT CTACCGGCCC TACCTGCAGA TCTTTGGGCA GATCCCTCAG
    GGGGAGATGG ACGTGGCCCT CATGGAGCAC GGCAACTGTT CACTGGGGCA GGGCTCATGG
    GGGCACCCCG AAGGGAACCT CTCTGGCAAA TGCGTCTCGC TTTATGCCAA CTGGCTGGTG
    GTGCTCCTCC TTGTTGTCTT CCTGCTGGTG GCCAACATCC TGCTGCTCAA TCTGCTCATT
    GCGATGTTCA GCCATACCTT CGGCAAAGTG CAGGGCAACA GCGACCTCTA CTGGAAGGCG
    CAGCGCTACA GCCTCATCCG GGAATTTCAC TCTCGGCCCG CACTGGCCCC GCCTCTCATT
    TTCATATCGC ACGTGCGCTC CCTCATCCAT CGATTTCGCA GGCGCCGGGC GCGCTTGCCA
    CCATCCCCAG ACGTCGAGCA TTTTCGGGTC CACCTCTCGA AGGAAGCGGA GCGGAGGCTG
    TTGACGTGGG AGTCAGTGCA AAAGGAGAAT TTCTTGCTGG CGCGTGCTCG GGACAAACGG
    GAGAGCAACT CTGAACGTCT GAAGCGCACG TCGCAGAAGG TGGACACAGC ACTGAAGCAG
    CTGACACAGA TCCGCGAGTA CGAGCAGCGT CTGAAAGGGC TGGAACTGGA GGTCCAGCAC
    TGTACCCGCG TCCTGGGCTG GGTAGCTGAG GCCCTGAGCC GTTCTGCATT GCTTCCCCCA
    GGGGGACCAC CCCCCCCAAT CCCACCTGGG CCCAAAGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225692
    Clone ID Related Accession (Same CDS sequence) XM_024979248.1
    Accession Version XM_024979248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3069bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGGCGTGG CCCCCTGGGG CGTGGTGCGG AATAGAGACA CTCTCACCAA CCCCAAGGGC 
    TCGTTCCCCG CAAGGTACCG GTGGCGCGGA GACCCAGAGG ATGGGGTCCA GTTCCCTCTT
    GACTACAACT ACTCGGCCTT CTTCCTGGTG GATGACGGCA CACACGGCCG CCTGGGCGGC
    GAGAACCGCT TCCGCTTGGG CTTCGAGTCC TACCTGGCCC AACAGAAGAC GGGCGTGGGA
    GGGACTGGCA TTGACATCCC TGTCCTCCTC CTCCTGATTG ATGGTGATGA GAAGATGTTG
    AAGCAGATAG AGGATGCCAC CCAGGCTCAG CTCCCCTGCC TCCTGGTGGC TGGATCTGGG
    GGAGCTGCGG ACTGCCTGGC AGAGATCCTG GAGGACACTC TGGCTCCAGG GAGTGGCGGG
    GGCAGGCGAC GTGAACCCCG AGATCTGATT AAGCGTTTCT TCCCCAAAGG GGACCCTGAG
    GTCCTGCAGG CCCAGGTGGA GAGGATCATG ACTCGGAAGG AGCTGCTGAC GGTCTATTCA
    ACTGATGACG GCCCTGAGGA ATTTGAGACC ATCGTCTTAA GGGCCCTTGT CAAGGCCTGT
    GGGAGCTCTG AAGCCTCAGC TTACCTGGAC GAGTTGCGTT TGGCTGTGGC TTGGAACCGT
    GTGGACATTG CCCAGAGTGA ACTCTTCCGG GGGGACATCC AATGGCGGTC CTTCCACCTG
    GAGGCCTCCC TCATGGACGC CCTCCTGAAC GACCGACCGG AGTTCGTGCG CTTGCTTATC
    TCCCATGGCC TCAGCCTGGG CCATTTCCTG ACTCCAGTGC GCCTGGCCCA GCTCTACAAC
    GCAGCGCCCC CCAACTCACT TATCCACAAC CTTCTGGACC AAGTGTCACA CGGTGCAAGC
    ACCAAAGCCC CAGCCCTTAA ACCCTCTGAG GAGCCACAGT TTCCTAAGGT GGGGCAGGTG
    CTGCGCATGC TGCTGGGGAA GACGTGCGCG CCGACGTTCC CCGCCGGGGG CACCCGCCGG
    AGCGACAACA GCAGAGAGAA TTCGTTTATG CTGTCAGACA AGGCTGCCTC TGGACTGATG
    GAAGCTGTCC CCGGGCAGGC CCCCTGGAGC GACCTGCTCC TCTGGGCACT GCTGCTCAAC
    AGGGCTCAGA TGGCCTTGTA CTTCTGGGAG ATGGGTTCCA ATTCTGTGGC CTCAGCTCTT
    GGGGCCTCTT TGTTGCTGCG AGTGTTGGGG CGCCTGGAGA CTGAGGCCGA GGAGGCGGCG
    CGGAGGAAGG AGCTGGCGGC CAAGTTCGAG GGGCTGGGTG TTGACCTCTT TGGAGAATGC
    TACCGCAGCA GCGAGGAACG GTCCGCCCGC CTGCTCCTTC GGCGCTGCCC ACTCTGGGGT
    GATGCCACTT GCCTGCAGCT CGCCACACAG GCGGATGCCC GTGCCTTCTT TGCCCAGGAT
    GGGGTACAGT CTCTGCTGAC ACAGAAGTGG TGGGGGGAGA TGGACAGCAC CACGCCCATC
    TGGGCCCTGG TTCTTGCCTT CTTTTGCCCC CCACTCATCT ACACCGACCT CATCACCTTC
    AGGAAGTCAG ATGAGGAGCC CACGCAGAAG GACCCGATGC TTGACGTGGA TGGCGACATC
    AATGGGGAAG GGCCTGTCGA GGCTACAGAG CCCCCTGAGA AGATGCCCTT GGGGGTACTG
    AGGCCGCCCA GACACAGGGA CTGCTGTGGG GGGTGCCTGC CGCGCCTGCG CCGCTGGCCG
    CAGTTCTGGG GGGCGCCGGT GACGGCCTTC ATGGGCAACG TGGTCAGCTA CCTCCTCTTC
    CTGCTGCTGT TTGCGCGCGT GCTGCTCATC GACTTCGAGC CGGAGAGGCC CAGCGTGCTG
    GAGCTGCTGC TCTACTTCTG GGCCTTCACC CTGCTCTGCG AGGAGTTCCG CCAGGGCCTG
    GGCAGCGGCC TGGGCAGCCT GGCCATGGGG GGACCCGGGA CAGACTCCCA CCGGGCCCCA
    CTGCGCCGTC GTCTGCAACT CTACCTCTCT GACGCCTGGA ACCAGTGCGA CCTGGTGGCC
    CTCGCCTGCT TCGTTCTGGG CGTGGGCTGC AGGCTGACCC CGGGCCTGTA TGACCTGGGC
    CGCACTGTCC TCTGCCTTGA CTTCATGATC TTCACGCTGA GGCTGCTGCA CATCTTCACA
    GTCAACAAAC AGCTGGGGCC CAAGATCGTC ATCGTGAGCA AGATGGTGAA GGATGTGTTC
    TTCTTCCTCT TCTTCCTCGG CGTGTGGCTG GTTGCCTACG GGGTGGCCAC TGAGGGGCTC
    CTTAGGCCCC AGGACCGTAG CCTCCCGAAT ATCCTGCGCC GTGTCTTCTA CCGGCCCTAC
    CTGCAGATCT TTGGGCAGAT CCCTCAGGGG GAGATGGACG TGGCCCTCAT GGAGCACGGC
    AACTGTTCAC TGGGGCAGGG CTCATGGGGG CACCCCGAAG GGAACCTCTC TGGCAAATGC
    GTCTCGCTTT ATGCCAACTG GCTGGTGGTG CTCCTCCTTG TTGTCTTCCT GCTGGTGGCC
    AACATCCTGC TGCTCAATCT GCTCATTGCG ATGTTCAGCC ATACCTTCGG CAAAGTGCAG
    GGCAACAGCG ACCTCTACTG GAAGGCGCAG CGCTACAGCC TCATCCGGGA ATTTCACTCT
    CGGCCCGCAC TGGCCCCGCC TCTCATTTTC ATATCGCACG TGCGCTCCCT CATCCATCGA
    TTTCGCAGGC GCCGGGCGCG CTTGCCACCA TCCCCAGACG TCGAGCATTT TCGGGTCCAC
    CTCTCGAAGG AAGCGGAGCG GAGGCTGTTG ACGTGGGAGT CAGTGCAAAA GGAGAATTTC
    TTGCTGGCGC GTGCTCGGGA CAAACGGGAG AGCAACTCTG AACGTCTGAA GCGCACGTCG
    CAGAAGGTGG ACACAGCACT GAAGCAGCTG ACACAGATCC GCGAGTACGA GCAGCGTCTG
    AAAGGGCTGG AACTGGAGGT CCAGCACTGT ACCCGCGTCC TGGGCTGGGT AGCTGAGGCC
    CTGAGCCGTT CTGCATTGCT TCCCCCAGGG GGACCACCCC CCCCAATCCC ACCTGGGCCC
    AAAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835016.1
    CDS246..3314
    Translation

    Target ORF information:

    RefSeq Version XM_024979248.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGGCGTGG CCCCCTGGGG CGTGGTGCGG AATAGAGACA CTCTCACCAA CCCCAAGGGC 
    TCGTTCCCCG CAAGGTACCG GTGGCGCGGA GACCCAGAGG ATGGGGTCCA GTTCCCTCTT
    GACTACAACT ACTCGGCCTT CTTCCTGGTG GATGACGGCA CACACGGCCG CCTGGGCGGC
    GAGAACCGCT TCCGCTTGGG CTTCGAGTCC TACCTGGCCC AACAGAAGAC GGGCGTGGGA
    GGGACTGGCA TTGACATCCC TGTCCTCCTC CTCCTGATTG ATGGTGATGA GAAGATGTTG
    AAGCAGATAG AGGATGCCAC CCAGGCTCAG CTCCCCTGCC TCCTGGTGGC TGGATCTGGG
    GGAGCTGCGG ACTGCCTGGC AGAGATCCTG GAGGACACTC TGGCTCCAGG GAGTGGCGGG
    GGCAGGCGAC GTGAACCCCG AGATCTGATT AAGCGTTTCT TCCCCAAAGG GGACCCTGAG
    GTCCTGCAGG CCCAGGTGGA GAGGATCATG ACTCGGAAGG AGCTGCTGAC GGTCTATTCA
    ACTGATGACG GCCCTGAGGA ATTTGAGACC ATCGTCTTAA GGGCCCTTGT CAAGGCCTGT
    GGGAGCTCTG AAGCCTCAGC TTACCTGGAC GAGTTGCGTT TGGCTGTGGC TTGGAACCGT
    GTGGACATTG CCCAGAGTGA ACTCTTCCGG GGGGACATCC AATGGCGGTC CTTCCACCTG
    GAGGCCTCCC TCATGGACGC CCTCCTGAAC GACCGACCGG AGTTCGTGCG CTTGCTTATC
    TCCCATGGCC TCAGCCTGGG CCATTTCCTG ACTCCAGTGC GCCTGGCCCA GCTCTACAAC
    GCAGCGCCCC CCAACTCACT TATCCACAAC CTTCTGGACC AAGTGTCACA CGGTGCAAGC
    ACCAAAGCCC CAGCCCTTAA ACCCTCTGAG GAGCCACAGT TTCCTAAGGT GGGGCAGGTG
    CTGCGCATGC TGCTGGGGAA GACGTGCGCG CCGACGTTCC CCGCCGGGGG CACCCGCCGG
    AGCGACAACA GCAGAGAGAA TTCGTTTATG CTGTCAGACA AGGCTGCCTC TGGACTGATG
    GAAGCTGTCC CCGGGCAGGC CCCCTGGAGC GACCTGCTCC TCTGGGCACT GCTGCTCAAC
    AGGGCTCAGA TGGCCTTGTA CTTCTGGGAG ATGGGTTCCA ATTCTGTGGC CTCAGCTCTT
    GGGGCCTCTT TGTTGCTGCG AGTGTTGGGG CGCCTGGAGA CTGAGGCCGA GGAGGCGGCG
    CGGAGGAAGG AGCTGGCGGC CAAGTTCGAG GGGCTGGGTG TTGACCTCTT TGGAGAATGC
    TACCGCAGCA GCGAGGAACG GTCCGCCCGC CTGCTCCTTC GGCGCTGCCC ACTCTGGGGT
    GATGCCACTT GCCTGCAGCT CGCCACACAG GCGGATGCCC GTGCCTTCTT TGCCCAGGAT
    GGGGTACAGT CTCTGCTGAC ACAGAAGTGG TGGGGGGAGA TGGACAGCAC CACGCCCATC
    TGGGCCCTGG TTCTTGCCTT CTTTTGCCCC CCACTCATCT ACACCGACCT CATCACCTTC
    AGGAAGTCAG ATGAGGAGCC CACGCAGAAG GACCCGATGC TTGACGTGGA TGGCGACATC
    AATGGGGAAG GGCCTGTCGA GGCTACAGAG CCCCCTGAGA AGATGCCCTT GGGGGTACTG
    AGGCCGCCCA GACACAGGGA CTGCTGTGGG GGGTGCCTGC CGCGCCTGCG CCGCTGGCCG
    CAGTTCTGGG GGGCGCCGGT GACGGCCTTC ATGGGCAACG TGGTCAGCTA CCTCCTCTTC
    CTGCTGCTGT TTGCGCGCGT GCTGCTCATC GACTTCGAGC CGGAGAGGCC CAGCGTGCTG
    GAGCTGCTGC TCTACTTCTG GGCCTTCACC CTGCTCTGCG AGGAGTTCCG CCAGGGCCTG
    GGCAGCGGCC TGGGCAGCCT GGCCATGGGG GGACCCGGGA CAGACTCCCA CCGGGCCCCA
    CTGCGCCGTC GTCTGCAACT CTACCTCTCT GACGCCTGGA ACCAGTGCGA CCTGGTGGCC
    CTCGCCTGCT TCGTTCTGGG CGTGGGCTGC AGGCTGACCC CGGGCCTGTA TGACCTGGGC
    CGCACTGTCC TCTGCCTTGA CTTCATGATC TTCACGCTGA GGCTGCTGCA CATCTTCACA
    GTCAACAAAC AGCTGGGGCC CAAGATCGTC ATCGTGAGCA AGATGGTGAA GGATGTGTTC
    TTCTTCCTCT TCTTCCTCGG CGTGTGGCTG GTTGCCTACG GGGTGGCCAC TGAGGGGCTC
    CTTAGGCCCC AGGACCGTAG CCTCCCGAAT ATCCTGCGCC GTGTCTTCTA CCGGCCCTAC
    CTGCAGATCT TTGGGCAGAT CCCTCAGGGG GAGATGGACG TGGCCCTCAT GGAGCACGGC
    AACTGTTCAC TGGGGCAGGG CTCATGGGGG CACCCCGAAG GGAACCTCTC TGGCAAATGC
    GTCTCGCTTT ATGCCAACTG GCTGGTGGTG CTCCTCCTTG TTGTCTTCCT GCTGGTGGCC
    AACATCCTGC TGCTCAATCT GCTCATTGCG ATGTTCAGCC ATACCTTCGG CAAAGTGCAG
    GGCAACAGCG ACCTCTACTG GAAGGCGCAG CGCTACAGCC TCATCCGGGA ATTTCACTCT
    CGGCCCGCAC TGGCCCCGCC TCTCATTTTC ATATCGCACG TGCGCTCCCT CATCCATCGA
    TTTCGCAGGC GCCGGGCGCG CTTGCCACCA TCCCCAGACG TCGAGCATTT TCGGGTCCAC
    CTCTCGAAGG AAGCGGAGCG GAGGCTGTTG ACGTGGGAGT CAGTGCAAAA GGAGAATTTC
    TTGCTGGCGC GTGCTCGGGA CAAACGGGAG AGCAACTCTG AACGTCTGAA GCGCACGTCG
    CAGAAGGTGG ACACAGCACT GAAGCAGCTG ACACAGATCC GCGAGTACGA GCAGCGTCTG
    AAAGGGCTGG AACTGGAGGT CCAGCACTGT ACCCGCGTCC TGGGCTGGGT AGCTGAGGCC
    CTGAGCCGTT CTGCATTGCT TCCCCCAGGG GGACCACCCC CCCCAATCCC ACCTGGGCCC
    AAAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa14297
    Clone ID Related Accession (Same CDS sequence) XM_002695205.5 , XM_002701936.5 , XM_002695205.6
    Accession Version XM_002695205.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002695205.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGCA GATAGAGGAT GCCACCCAGG CTCAGCTCCC CTGCCTCCTG
    GTGGCTGGAT CTGGGGGAGC TGCGGACTGC CTGGCAGAGA TCCTGGAGGA CACTCTGGCT
    CCAGGGAGTG GCGGGGGCAG GCGACGTGAA CCCCGAGATC TGATTAAGCG TTTCTTCCCC
    AAAGGGGACC CTGAGGTCCT GCAGGCCCAG GTGGAGAGGA TCATGACTCG GAAGGAGCTG
    CTGACGGTCT ATTCAACTGA TGACGGCCCT GAGGAATTTG AGACCATCGT CTTAAGGGCC
    CTTGTCAAGG CCTGTGGGAG CTCTGAAGCC TCAGCTTACC TGGACGAGTT GCGTTTGGCT
    GTGGCTTGGA ACCGTGTGGA CATTGCCCAG AGTGAACTCT TCCGGGGGGA CATCCAATGG
    CGGTCCTTCC ACCTGGAGGC CTCCCTCATG GACGCCCTCC TGAACGACCG ACCGGAGTTC
    GTGCGCTTGC TTATCTCCCA TGGCCTCAGC CTGGGCCATT TCCTGACTCC AGTGCGCCTG
    GCCCAGCTCT ACAACGCAGC GCCCCCCAAC TCACTTATCC ACAACCTTCT GGACCAAGTG
    TCACACGGTG CAAGCACCAA AGCCCCAGCC CTTAAACCCT CTGAGGAGCC ACAGTTTCCT
    AAGGTGGGGC AGGTGCTGCG CATGCTGCTG GGGAAGACGT GCGCGCCGAC GTTCCCCGCC
    GGGGGCACCC GCCGGAGCGA CAACAGCAGA GAGAATTCGT TTATGCTGTC AGACAAGGCT
    GCCTCTGGAC TGATGGAAGC TGTCCCCGGG CAGGCCCCCT GGAGCGACCT GCTCCTCTGG
    GCACTGCTGC TCAACAGGGC TCAGATGGCC TTGTACTTCT GGGAGATGGG TTCCAATTCT
    GTGGCCTCAG CTCTTGGGGC CTCTTTGTTG CTGCGAGTGT TGGGGCGCCT GGAGACTGAG
    GCCGAGGAGG CGGCGCGGAG GAAGGAGCTG GCGGCCAAGT TCGAGGGGCT GGGTGTTGAC
    CTCTTTGGAG AATGCTACCG CAGCAGCGAG GAACGGTCCG CCCGCCTGCT CCTTCGGCGC
    TGCCCACTCT GGGGTGATGC CACTTGCCTG CAGCTCGCCA CACAGGCGGA TGCCCGTGCC
    TTCTTTGCCC AGGATGGGGT ACAGTCTCTG CTGACACAGA AGTGGTGGGG GGAGATGGAC
    AGCACCACGC CCATCTGGGC CCTGGTTCTT GCCTTCTTTT GCCCCCCACT CATCTACACC
    GACCTCATCA CCTTCAGGAA GTCAGATGAG GAGCCCACGC AGAAGGACCC GATGCTTGAC
    GTGGATGGCG ACATCAATGG GGAAGGGCCT GTCGAGGCTA CAGAGCCCCC TGAGAAGATG
    CCCTTGGGGG TACTGAGGCC GCCCAGACAC AGGGACTGCT GTGGGGGGTG CCTGCCGCGC
    CTGCGCCGCT GGCCGCAGTT CTGGGGGGCG CCGGTGACGG CCTTCATGGG CAACGTGGTC
    AGCTACCTCC TCTTCCTGCT GCTGTTTGCG CGCGTGCTGC TCATCGACTT CGAGCCGGAG
    AGGCCCAGCG TGCTGGAGCT GCTGCTCTAC TTCTGGGCCT TCACCCTGCT CTGCGAGGAG
    TTCCGCCAGG GCCTGGGCAG CGGCCTGGGC AGCCTGGCCA TGGGGGGACC CGGGACAGAC
    TCCCACCGGG CCCCACTGCG CCGTCGTCTG CAACTCTACC TCTCTGACGC CTGGAACCAG
    TGCGACCTGG TGGCCCTCGC CTGCTTCGTT CTGGGCGTGG GCTGCAGGCT GACCCCGGGC
    CTGTATGACC TGGGCCGCAC TGTCCTCTGC CTTGACTTCA TGATCTTCAC GCTGAGGCTG
    CTGCACATCT TCACAGTCAA CAAACAGCTG GGGCCCAAGA TCGTCATCGT GAGCAAGATG
    GTGAAGGATG TGTTCTTCTT CCTCTTCTTC CTCGGCGTGT GGCTGGTTGC CTACGGGGTG
    GCCACTGAGG GGCTCCTTAG GCCCCAGGAC CGTAGCCTCC CGAATATCCT GCGCCGTGTC
    TTCTACCGGC CCTACCTGCA GATCTTTGGG CAGATCCCTC AGGGGGAGAT GGACGTGGCC
    CTCATGGAGC ACGGCAACTG TTCACTGGGG CAGGGCTCAT GGGGGCACCC CGAAGGGAAC
    CTCTCTGGCA AATGCGTCTC GCTTTATGCC AACTGGCTGG TGGTGCTCCT CCTTGTTGTC
    TTCCTGCTGG TGGCCAACAT CCTGCTGCTC AATCTGCTCA TTGCGATGTT CAGCCATACC
    TTCGGCAAAG TGCAGGGCAA CAGCGACCTC TACTGGAAGG CGCAGCGCTA CAGCCTCATC
    CGGGAATTTC ACTCTCGGCC CGCACTGGCC CCGCCTCTCA TTTTCATATC GCACGTGCGC
    TCCCTCATCC ATCGATTTCG CAGGCGCCGG GCGCGCTTGC CACCATCCCC AGACGTCGAG
    CATTTTCGGG TCCACCTCTC GAAGGAAGCG GAGCGGAGGC TGTTGACGTG GGAGTCAGTG
    CAAAAGGAGA ATTTCTTGCT GGCGCGTGCT CGGGACAAAC GGGAGAGCAA CTCTGAACGT
    CTGAAGCGCA CGTCGCAGAA GGTGGACACA GCACTGAAGC AGCTGACACA GATCCGCGAG
    TACGAGCAGC GTCTGAAAGG GCTGGAACTG GAGGTCCAGC ACTGTACCCG CGTCCTGGGC
    TGGGTAGCTG AGGCCCTGAG CCGTTCTGCA TTGCTTCCCC CAGGGGGACC ACCCCCCCCA
    ATCCCACCTG GGCCCAAAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002695251.2
    CDS110..3733
    Translation

    Target ORF information:

    RefSeq Version XM_002695205.5
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002695205.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGCA GATAGAGGAT GCCACCCAGG CTCAGCTCCC CTGCCTCCTG
    GTGGCTGGAT CTGGGGGAGC TGCGGACTGC CTGGCAGAGA TCCTGGAGGA CACTCTGGCT
    CCAGGGAGTG GCGGGGGCAG GCGACGTGAA CCCCGAGATC TGATTAAGCG TTTCTTCCCC
    AAAGGGGACC CTGAGGTCCT GCAGGCCCAG GTGGAGAGGA TCATGACTCG GAAGGAGCTG
    CTGACGGTCT ATTCAACTGA TGACGGCCCT GAGGAATTTG AGACCATCGT CTTAAGGGCC
    CTTGTCAAGG CCTGTGGGAG CTCTGAAGCC TCAGCTTACC TGGACGAGTT GCGTTTGGCT
    GTGGCTTGGA ACCGTGTGGA CATTGCCCAG AGTGAACTCT TCCGGGGGGA CATCCAATGG
    CGGTCCTTCC ACCTGGAGGC CTCCCTCATG GACGCCCTCC TGAACGACCG ACCGGAGTTC
    GTGCGCTTGC TTATCTCCCA TGGCCTCAGC CTGGGCCATT TCCTGACTCC AGTGCGCCTG
    GCCCAGCTCT ACAACGCAGC GCCCCCCAAC TCACTTATCC ACAACCTTCT GGACCAAGTG
    TCACACGGTG CAAGCACCAA AGCCCCAGCC CTTAAACCCT CTGAGGAGCC ACAGTTTCCT
    AAGGTGGGGC AGGTGCTGCG CATGCTGCTG GGGAAGACGT GCGCGCCGAC GTTCCCCGCC
    GGGGGCACCC GCCGGAGCGA CAACAGCAGA GAGAATTCGT TTATGCTGTC AGACAAGGCT
    GCCTCTGGAC TGATGGAAGC TGTCCCCGGG CAGGCCCCCT GGAGCGACCT GCTCCTCTGG
    GCACTGCTGC TCAACAGGGC TCAGATGGCC TTGTACTTCT GGGAGATGGG TTCCAATTCT
    GTGGCCTCAG CTCTTGGGGC CTCTTTGTTG CTGCGAGTGT TGGGGCGCCT GGAGACTGAG
    GCCGAGGAGG CGGCGCGGAG GAAGGAGCTG GCGGCCAAGT TCGAGGGGCT GGGTGTTGAC
    CTCTTTGGAG AATGCTACCG CAGCAGCGAG GAACGGTCCG CCCGCCTGCT CCTTCGGCGC
    TGCCCACTCT GGGGTGATGC CACTTGCCTG CAGCTCGCCA CACAGGCGGA TGCCCGTGCC
    TTCTTTGCCC AGGATGGGGT ACAGTCTCTG CTGACACAGA AGTGGTGGGG GGAGATGGAC
    AGCACCACGC CCATCTGGGC CCTGGTTCTT GCCTTCTTTT GCCCCCCACT CATCTACACC
    GACCTCATCA CCTTCAGGAA GTCAGATGAG GAGCCCACGC AGAAGGACCC GATGCTTGAC
    GTGGATGGCG ACATCAATGG GGAAGGGCCT GTCGAGGCTA CAGAGCCCCC TGAGAAGATG
    CCCTTGGGGG TACTGAGGCC GCCCAGACAC AGGGACTGCT GTGGGGGGTG CCTGCCGCGC
    CTGCGCCGCT GGCCGCAGTT CTGGGGGGCG CCGGTGACGG CCTTCATGGG CAACGTGGTC
    AGCTACCTCC TCTTCCTGCT GCTGTTTGCG CGCGTGCTGC TCATCGACTT CGAGCCGGAG
    AGGCCCAGCG TGCTGGAGCT GCTGCTCTAC TTCTGGGCCT TCACCCTGCT CTGCGAGGAG
    TTCCGCCAGG GCCTGGGCAG CGGCCTGGGC AGCCTGGCCA TGGGGGGACC CGGGACAGAC
    TCCCACCGGG CCCCACTGCG CCGTCGTCTG CAACTCTACC TCTCTGACGC CTGGAACCAG
    TGCGACCTGG TGGCCCTCGC CTGCTTCGTT CTGGGCGTGG GCTGCAGGCT GACCCCGGGC
    CTGTATGACC TGGGCCGCAC TGTCCTCTGC CTTGACTTCA TGATCTTCAC GCTGAGGCTG
    CTGCACATCT TCACAGTCAA CAAACAGCTG GGGCCCAAGA TCGTCATCGT GAGCAAGATG
    GTGAAGGATG TGTTCTTCTT CCTCTTCTTC CTCGGCGTGT GGCTGGTTGC CTACGGGGTG
    GCCACTGAGG GGCTCCTTAG GCCCCAGGAC CGTAGCCTCC CGAATATCCT GCGCCGTGTC
    TTCTACCGGC CCTACCTGCA GATCTTTGGG CAGATCCCTC AGGGGGAGAT GGACGTGGCC
    CTCATGGAGC ACGGCAACTG TTCACTGGGG CAGGGCTCAT GGGGGCACCC CGAAGGGAAC
    CTCTCTGGCA AATGCGTCTC GCTTTATGCC AACTGGCTGG TGGTGCTCCT CCTTGTTGTC
    TTCCTGCTGG TGGCCAACAT CCTGCTGCTC AATCTGCTCA TTGCGATGTT CAGCCATACC
    TTCGGCAAAG TGCAGGGCAA CAGCGACCTC TACTGGAAGG CGCAGCGCTA CAGCCTCATC
    CGGGAATTTC ACTCTCGGCC CGCACTGGCC CCGCCTCTCA TTTTCATATC GCACGTGCGC
    TCCCTCATCC ATCGATTTCG CAGGCGCCGG GCGCGCTTGC CACCATCCCC AGACGTCGAG
    CATTTTCGGG TCCACCTCTC GAAGGAAGCG GAGCGGAGGC TGTTGACGTG GGAGTCAGTG
    CAAAAGGAGA ATTTCTTGCT GGCGCGTGCT CGGGACAAAC GGGAGAGCAA CTCTGAACGT
    CTGAAGCGCA CGTCGCAGAA GGTGGACACA GCACTGAAGC AGCTGACACA GATCCGCGAG
    TACGAGCAGC GTCTGAAAGG GCTGGAACTG GAGGTCCAGC ACTGTACCCG CGTCCTGGGC
    TGGGTAGCTG AGGCCCTGAG CCGTTCTGCA TTGCTTCCCC CAGGGGGACC ACCCCCCCCA
    ATCCCACCTG GGCCCAAAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa14297
    Clone ID Related Accession (Same CDS sequence) XM_002695205.5 , XM_002701936.5 , XM_002695205.6
    Accession Version XM_002701936.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002701936.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGCA GATAGAGGAT GCCACCCAGG CTCAGCTCCC CTGCCTCCTG
    GTGGCTGGAT CTGGGGGAGC TGCGGACTGC CTGGCAGAGA TCCTGGAGGA CACTCTGGCT
    CCAGGGAGTG GCGGGGGCAG GCGACGTGAA CCCCGAGATC TGATTAAGCG TTTCTTCCCC
    AAAGGGGACC CTGAGGTCCT GCAGGCCCAG GTGGAGAGGA TCATGACTCG GAAGGAGCTG
    CTGACGGTCT ATTCAACTGA TGACGGCCCT GAGGAATTTG AGACCATCGT CTTAAGGGCC
    CTTGTCAAGG CCTGTGGGAG CTCTGAAGCC TCAGCTTACC TGGACGAGTT GCGTTTGGCT
    GTGGCTTGGA ACCGTGTGGA CATTGCCCAG AGTGAACTCT TCCGGGGGGA CATCCAATGG
    CGGTCCTTCC ACCTGGAGGC CTCCCTCATG GACGCCCTCC TGAACGACCG ACCGGAGTTC
    GTGCGCTTGC TTATCTCCCA TGGCCTCAGC CTGGGCCATT TCCTGACTCC AGTGCGCCTG
    GCCCAGCTCT ACAACGCAGC GCCCCCCAAC TCACTTATCC ACAACCTTCT GGACCAAGTG
    TCACACGGTG CAAGCACCAA AGCCCCAGCC CTTAAACCCT CTGAGGAGCC ACAGTTTCCT
    AAGGTGGGGC AGGTGCTGCG CATGCTGCTG GGGAAGACGT GCGCGCCGAC GTTCCCCGCC
    GGGGGCACCC GCCGGAGCGA CAACAGCAGA GAGAATTCGT TTATGCTGTC AGACAAGGCT
    GCCTCTGGAC TGATGGAAGC TGTCCCCGGG CAGGCCCCCT GGAGCGACCT GCTCCTCTGG
    GCACTGCTGC TCAACAGGGC TCAGATGGCC TTGTACTTCT GGGAGATGGG TTCCAATTCT
    GTGGCCTCAG CTCTTGGGGC CTCTTTGTTG CTGCGAGTGT TGGGGCGCCT GGAGACTGAG
    GCCGAGGAGG CGGCGCGGAG GAAGGAGCTG GCGGCCAAGT TCGAGGGGCT GGGTGTTGAC
    CTCTTTGGAG AATGCTACCG CAGCAGCGAG GAACGGTCCG CCCGCCTGCT CCTTCGGCGC
    TGCCCACTCT GGGGTGATGC CACTTGCCTG CAGCTCGCCA CACAGGCGGA TGCCCGTGCC
    TTCTTTGCCC AGGATGGGGT ACAGTCTCTG CTGACACAGA AGTGGTGGGG GGAGATGGAC
    AGCACCACGC CCATCTGGGC CCTGGTTCTT GCCTTCTTTT GCCCCCCACT CATCTACACC
    GACCTCATCA CCTTCAGGAA GTCAGATGAG GAGCCCACGC AGAAGGACCC GATGCTTGAC
    GTGGATGGCG ACATCAATGG GGAAGGGCCT GTCGAGGCTA CAGAGCCCCC TGAGAAGATG
    CCCTTGGGGG TACTGAGGCC GCCCAGACAC AGGGACTGCT GTGGGGGGTG CCTGCCGCGC
    CTGCGCCGCT GGCCGCAGTT CTGGGGGGCG CCGGTGACGG CCTTCATGGG CAACGTGGTC
    AGCTACCTCC TCTTCCTGCT GCTGTTTGCG CGCGTGCTGC TCATCGACTT CGAGCCGGAG
    AGGCCCAGCG TGCTGGAGCT GCTGCTCTAC TTCTGGGCCT TCACCCTGCT CTGCGAGGAG
    TTCCGCCAGG GCCTGGGCAG CGGCCTGGGC AGCCTGGCCA TGGGGGGACC CGGGACAGAC
    TCCCACCGGG CCCCACTGCG CCGTCGTCTG CAACTCTACC TCTCTGACGC CTGGAACCAG
    TGCGACCTGG TGGCCCTCGC CTGCTTCGTT CTGGGCGTGG GCTGCAGGCT GACCCCGGGC
    CTGTATGACC TGGGCCGCAC TGTCCTCTGC CTTGACTTCA TGATCTTCAC GCTGAGGCTG
    CTGCACATCT TCACAGTCAA CAAACAGCTG GGGCCCAAGA TCGTCATCGT GAGCAAGATG
    GTGAAGGATG TGTTCTTCTT CCTCTTCTTC CTCGGCGTGT GGCTGGTTGC CTACGGGGTG
    GCCACTGAGG GGCTCCTTAG GCCCCAGGAC CGTAGCCTCC CGAATATCCT GCGCCGTGTC
    TTCTACCGGC CCTACCTGCA GATCTTTGGG CAGATCCCTC AGGGGGAGAT GGACGTGGCC
    CTCATGGAGC ACGGCAACTG TTCACTGGGG CAGGGCTCAT GGGGGCACCC CGAAGGGAAC
    CTCTCTGGCA AATGCGTCTC GCTTTATGCC AACTGGCTGG TGGTGCTCCT CCTTGTTGTC
    TTCCTGCTGG TGGCCAACAT CCTGCTGCTC AATCTGCTCA TTGCGATGTT CAGCCATACC
    TTCGGCAAAG TGCAGGGCAA CAGCGACCTC TACTGGAAGG CGCAGCGCTA CAGCCTCATC
    CGGGAATTTC ACTCTCGGCC CGCACTGGCC CCGCCTCTCA TTTTCATATC GCACGTGCGC
    TCCCTCATCC ATCGATTTCG CAGGCGCCGG GCGCGCTTGC CACCATCCCC AGACGTCGAG
    CATTTTCGGG TCCACCTCTC GAAGGAAGCG GAGCGGAGGC TGTTGACGTG GGAGTCAGTG
    CAAAAGGAGA ATTTCTTGCT GGCGCGTGCT CGGGACAAAC GGGAGAGCAA CTCTGAACGT
    CTGAAGCGCA CGTCGCAGAA GGTGGACACA GCACTGAAGC AGCTGACACA GATCCGCGAG
    TACGAGCAGC GTCTGAAAGG GCTGGAACTG GAGGTCCAGC ACTGTACCCG CGTCCTGGGC
    TGGGTAGCTG AGGCCCTGAG CCGTTCTGCA TTGCTTCCCC CAGGGGGACC ACCCCCCCCA
    ATCCCACCTG GGCCCAAAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002701982.2
    CDS110..3733
    Translation

    Target ORF information:

    RefSeq Version XM_002701936.5
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002701936.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGCA GATAGAGGAT GCCACCCAGG CTCAGCTCCC CTGCCTCCTG
    GTGGCTGGAT CTGGGGGAGC TGCGGACTGC CTGGCAGAGA TCCTGGAGGA CACTCTGGCT
    CCAGGGAGTG GCGGGGGCAG GCGACGTGAA CCCCGAGATC TGATTAAGCG TTTCTTCCCC
    AAAGGGGACC CTGAGGTCCT GCAGGCCCAG GTGGAGAGGA TCATGACTCG GAAGGAGCTG
    CTGACGGTCT ATTCAACTGA TGACGGCCCT GAGGAATTTG AGACCATCGT CTTAAGGGCC
    CTTGTCAAGG CCTGTGGGAG CTCTGAAGCC TCAGCTTACC TGGACGAGTT GCGTTTGGCT
    GTGGCTTGGA ACCGTGTGGA CATTGCCCAG AGTGAACTCT TCCGGGGGGA CATCCAATGG
    CGGTCCTTCC ACCTGGAGGC CTCCCTCATG GACGCCCTCC TGAACGACCG ACCGGAGTTC
    GTGCGCTTGC TTATCTCCCA TGGCCTCAGC CTGGGCCATT TCCTGACTCC AGTGCGCCTG
    GCCCAGCTCT ACAACGCAGC GCCCCCCAAC TCACTTATCC ACAACCTTCT GGACCAAGTG
    TCACACGGTG CAAGCACCAA AGCCCCAGCC CTTAAACCCT CTGAGGAGCC ACAGTTTCCT
    AAGGTGGGGC AGGTGCTGCG CATGCTGCTG GGGAAGACGT GCGCGCCGAC GTTCCCCGCC
    GGGGGCACCC GCCGGAGCGA CAACAGCAGA GAGAATTCGT TTATGCTGTC AGACAAGGCT
    GCCTCTGGAC TGATGGAAGC TGTCCCCGGG CAGGCCCCCT GGAGCGACCT GCTCCTCTGG
    GCACTGCTGC TCAACAGGGC TCAGATGGCC TTGTACTTCT GGGAGATGGG TTCCAATTCT
    GTGGCCTCAG CTCTTGGGGC CTCTTTGTTG CTGCGAGTGT TGGGGCGCCT GGAGACTGAG
    GCCGAGGAGG CGGCGCGGAG GAAGGAGCTG GCGGCCAAGT TCGAGGGGCT GGGTGTTGAC
    CTCTTTGGAG AATGCTACCG CAGCAGCGAG GAACGGTCCG CCCGCCTGCT CCTTCGGCGC
    TGCCCACTCT GGGGTGATGC CACTTGCCTG CAGCTCGCCA CACAGGCGGA TGCCCGTGCC
    TTCTTTGCCC AGGATGGGGT ACAGTCTCTG CTGACACAGA AGTGGTGGGG GGAGATGGAC
    AGCACCACGC CCATCTGGGC CCTGGTTCTT GCCTTCTTTT GCCCCCCACT CATCTACACC
    GACCTCATCA CCTTCAGGAA GTCAGATGAG GAGCCCACGC AGAAGGACCC GATGCTTGAC
    GTGGATGGCG ACATCAATGG GGAAGGGCCT GTCGAGGCTA CAGAGCCCCC TGAGAAGATG
    CCCTTGGGGG TACTGAGGCC GCCCAGACAC AGGGACTGCT GTGGGGGGTG CCTGCCGCGC
    CTGCGCCGCT GGCCGCAGTT CTGGGGGGCG CCGGTGACGG CCTTCATGGG CAACGTGGTC
    AGCTACCTCC TCTTCCTGCT GCTGTTTGCG CGCGTGCTGC TCATCGACTT CGAGCCGGAG
    AGGCCCAGCG TGCTGGAGCT GCTGCTCTAC TTCTGGGCCT TCACCCTGCT CTGCGAGGAG
    TTCCGCCAGG GCCTGGGCAG CGGCCTGGGC AGCCTGGCCA TGGGGGGACC CGGGACAGAC
    TCCCACCGGG CCCCACTGCG CCGTCGTCTG CAACTCTACC TCTCTGACGC CTGGAACCAG
    TGCGACCTGG TGGCCCTCGC CTGCTTCGTT CTGGGCGTGG GCTGCAGGCT GACCCCGGGC
    CTGTATGACC TGGGCCGCAC TGTCCTCTGC CTTGACTTCA TGATCTTCAC GCTGAGGCTG
    CTGCACATCT TCACAGTCAA CAAACAGCTG GGGCCCAAGA TCGTCATCGT GAGCAAGATG
    GTGAAGGATG TGTTCTTCTT CCTCTTCTTC CTCGGCGTGT GGCTGGTTGC CTACGGGGTG
    GCCACTGAGG GGCTCCTTAG GCCCCAGGAC CGTAGCCTCC CGAATATCCT GCGCCGTGTC
    TTCTACCGGC CCTACCTGCA GATCTTTGGG CAGATCCCTC AGGGGGAGAT GGACGTGGCC
    CTCATGGAGC ACGGCAACTG TTCACTGGGG CAGGGCTCAT GGGGGCACCC CGAAGGGAAC
    CTCTCTGGCA AATGCGTCTC GCTTTATGCC AACTGGCTGG TGGTGCTCCT CCTTGTTGTC
    TTCCTGCTGG TGGCCAACAT CCTGCTGCTC AATCTGCTCA TTGCGATGTT CAGCCATACC
    TTCGGCAAAG TGCAGGGCAA CAGCGACCTC TACTGGAAGG CGCAGCGCTA CAGCCTCATC
    CGGGAATTTC ACTCTCGGCC CGCACTGGCC CCGCCTCTCA TTTTCATATC GCACGTGCGC
    TCCCTCATCC ATCGATTTCG CAGGCGCCGG GCGCGCTTGC CACCATCCCC AGACGTCGAG
    CATTTTCGGG TCCACCTCTC GAAGGAAGCG GAGCGGAGGC TGTTGACGTG GGAGTCAGTG
    CAAAAGGAGA ATTTCTTGCT GGCGCGTGCT CGGGACAAAC GGGAGAGCAA CTCTGAACGT
    CTGAAGCGCA CGTCGCAGAA GGTGGACACA GCACTGAAGC AGCTGACACA GATCCGCGAG
    TACGAGCAGC GTCTGAAAGG GCTGGAACTG GAGGTCCAGC ACTGTACCCG CGTCCTGGGC
    TGGGTAGCTG AGGCCCTGAG CCGTTCTGCA TTGCTTCCCC CAGGGGGACC ACCCCCCCCA
    ATCCCACCTG GGCCCAAAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa14297
    Clone ID Related Accession (Same CDS sequence) XM_002695205.5 , XM_002701936.5 , XM_002695205.6
    Accession Version XM_002695205.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002695205.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGCA GATAGAGGAT GCCACCCAGG CTCAGCTCCC CTGCCTCCTG
    GTGGCTGGAT CTGGGGGAGC TGCGGACTGC CTGGCAGAGA TCCTGGAGGA CACTCTGGCT
    CCAGGGAGTG GCGGGGGCAG GCGACGTGAA CCCCGAGATC TGATTAAGCG TTTCTTCCCC
    AAAGGGGACC CTGAGGTCCT GCAGGCCCAG GTGGAGAGGA TCATGACTCG GAAGGAGCTG
    CTGACGGTCT ATTCAACTGA TGACGGCCCT GAGGAATTTG AGACCATCGT CTTAAGGGCC
    CTTGTCAAGG CCTGTGGGAG CTCTGAAGCC TCAGCTTACC TGGACGAGTT GCGTTTGGCT
    GTGGCTTGGA ACCGTGTGGA CATTGCCCAG AGTGAACTCT TCCGGGGGGA CATCCAATGG
    CGGTCCTTCC ACCTGGAGGC CTCCCTCATG GACGCCCTCC TGAACGACCG ACCGGAGTTC
    GTGCGCTTGC TTATCTCCCA TGGCCTCAGC CTGGGCCATT TCCTGACTCC AGTGCGCCTG
    GCCCAGCTCT ACAACGCAGC GCCCCCCAAC TCACTTATCC ACAACCTTCT GGACCAAGTG
    TCACACGGTG CAAGCACCAA AGCCCCAGCC CTTAAACCCT CTGAGGAGCC ACAGTTTCCT
    AAGGTGGGGC AGGTGCTGCG CATGCTGCTG GGGAAGACGT GCGCGCCGAC GTTCCCCGCC
    GGGGGCACCC GCCGGAGCGA CAACAGCAGA GAGAATTCGT TTATGCTGTC AGACAAGGCT
    GCCTCTGGAC TGATGGAAGC TGTCCCCGGG CAGGCCCCCT GGAGCGACCT GCTCCTCTGG
    GCACTGCTGC TCAACAGGGC TCAGATGGCC TTGTACTTCT GGGAGATGGG TTCCAATTCT
    GTGGCCTCAG CTCTTGGGGC CTCTTTGTTG CTGCGAGTGT TGGGGCGCCT GGAGACTGAG
    GCCGAGGAGG CGGCGCGGAG GAAGGAGCTG GCGGCCAAGT TCGAGGGGCT GGGTGTTGAC
    CTCTTTGGAG AATGCTACCG CAGCAGCGAG GAACGGTCCG CCCGCCTGCT CCTTCGGCGC
    TGCCCACTCT GGGGTGATGC CACTTGCCTG CAGCTCGCCA CACAGGCGGA TGCCCGTGCC
    TTCTTTGCCC AGGATGGGGT ACAGTCTCTG CTGACACAGA AGTGGTGGGG GGAGATGGAC
    AGCACCACGC CCATCTGGGC CCTGGTTCTT GCCTTCTTTT GCCCCCCACT CATCTACACC
    GACCTCATCA CCTTCAGGAA GTCAGATGAG GAGCCCACGC AGAAGGACCC GATGCTTGAC
    GTGGATGGCG ACATCAATGG GGAAGGGCCT GTCGAGGCTA CAGAGCCCCC TGAGAAGATG
    CCCTTGGGGG TACTGAGGCC GCCCAGACAC AGGGACTGCT GTGGGGGGTG CCTGCCGCGC
    CTGCGCCGCT GGCCGCAGTT CTGGGGGGCG CCGGTGACGG CCTTCATGGG CAACGTGGTC
    AGCTACCTCC TCTTCCTGCT GCTGTTTGCG CGCGTGCTGC TCATCGACTT CGAGCCGGAG
    AGGCCCAGCG TGCTGGAGCT GCTGCTCTAC TTCTGGGCCT TCACCCTGCT CTGCGAGGAG
    TTCCGCCAGG GCCTGGGCAG CGGCCTGGGC AGCCTGGCCA TGGGGGGACC CGGGACAGAC
    TCCCACCGGG CCCCACTGCG CCGTCGTCTG CAACTCTACC TCTCTGACGC CTGGAACCAG
    TGCGACCTGG TGGCCCTCGC CTGCTTCGTT CTGGGCGTGG GCTGCAGGCT GACCCCGGGC
    CTGTATGACC TGGGCCGCAC TGTCCTCTGC CTTGACTTCA TGATCTTCAC GCTGAGGCTG
    CTGCACATCT TCACAGTCAA CAAACAGCTG GGGCCCAAGA TCGTCATCGT GAGCAAGATG
    GTGAAGGATG TGTTCTTCTT CCTCTTCTTC CTCGGCGTGT GGCTGGTTGC CTACGGGGTG
    GCCACTGAGG GGCTCCTTAG GCCCCAGGAC CGTAGCCTCC CGAATATCCT GCGCCGTGTC
    TTCTACCGGC CCTACCTGCA GATCTTTGGG CAGATCCCTC AGGGGGAGAT GGACGTGGCC
    CTCATGGAGC ACGGCAACTG TTCACTGGGG CAGGGCTCAT GGGGGCACCC CGAAGGGAAC
    CTCTCTGGCA AATGCGTCTC GCTTTATGCC AACTGGCTGG TGGTGCTCCT CCTTGTTGTC
    TTCCTGCTGG TGGCCAACAT CCTGCTGCTC AATCTGCTCA TTGCGATGTT CAGCCATACC
    TTCGGCAAAG TGCAGGGCAA CAGCGACCTC TACTGGAAGG CGCAGCGCTA CAGCCTCATC
    CGGGAATTTC ACTCTCGGCC CGCACTGGCC CCGCCTCTCA TTTTCATATC GCACGTGCGC
    TCCCTCATCC ATCGATTTCG CAGGCGCCGG GCGCGCTTGC CACCATCCCC AGACGTCGAG
    CATTTTCGGG TCCACCTCTC GAAGGAAGCG GAGCGGAGGC TGTTGACGTG GGAGTCAGTG
    CAAAAGGAGA ATTTCTTGCT GGCGCGTGCT CGGGACAAAC GGGAGAGCAA CTCTGAACGT
    CTGAAGCGCA CGTCGCAGAA GGTGGACACA GCACTGAAGC AGCTGACACA GATCCGCGAG
    TACGAGCAGC GTCTGAAAGG GCTGGAACTG GAGGTCCAGC ACTGTACCCG CGTCCTGGGC
    TGGGTAGCTG AGGCCCTGAG CCGTTCTGCA TTGCTTCCCC CAGGGGGACC ACCCCCCCCA
    ATCCCACCTG GGCCCAAAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002695251.2
    CDS128..3751
    Translation

    Target ORF information:

    RefSeq Version XM_002695205.6
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002695205.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGCA GATAGAGGAT GCCACCCAGG CTCAGCTCCC CTGCCTCCTG
    GTGGCTGGAT CTGGGGGAGC TGCGGACTGC CTGGCAGAGA TCCTGGAGGA CACTCTGGCT
    CCAGGGAGTG GCGGGGGCAG GCGACGTGAA CCCCGAGATC TGATTAAGCG TTTCTTCCCC
    AAAGGGGACC CTGAGGTCCT GCAGGCCCAG GTGGAGAGGA TCATGACTCG GAAGGAGCTG
    CTGACGGTCT ATTCAACTGA TGACGGCCCT GAGGAATTTG AGACCATCGT CTTAAGGGCC
    CTTGTCAAGG CCTGTGGGAG CTCTGAAGCC TCAGCTTACC TGGACGAGTT GCGTTTGGCT
    GTGGCTTGGA ACCGTGTGGA CATTGCCCAG AGTGAACTCT TCCGGGGGGA CATCCAATGG
    CGGTCCTTCC ACCTGGAGGC CTCCCTCATG GACGCCCTCC TGAACGACCG ACCGGAGTTC
    GTGCGCTTGC TTATCTCCCA TGGCCTCAGC CTGGGCCATT TCCTGACTCC AGTGCGCCTG
    GCCCAGCTCT ACAACGCAGC GCCCCCCAAC TCACTTATCC ACAACCTTCT GGACCAAGTG
    TCACACGGTG CAAGCACCAA AGCCCCAGCC CTTAAACCCT CTGAGGAGCC ACAGTTTCCT
    AAGGTGGGGC AGGTGCTGCG CATGCTGCTG GGGAAGACGT GCGCGCCGAC GTTCCCCGCC
    GGGGGCACCC GCCGGAGCGA CAACAGCAGA GAGAATTCGT TTATGCTGTC AGACAAGGCT
    GCCTCTGGAC TGATGGAAGC TGTCCCCGGG CAGGCCCCCT GGAGCGACCT GCTCCTCTGG
    GCACTGCTGC TCAACAGGGC TCAGATGGCC TTGTACTTCT GGGAGATGGG TTCCAATTCT
    GTGGCCTCAG CTCTTGGGGC CTCTTTGTTG CTGCGAGTGT TGGGGCGCCT GGAGACTGAG
    GCCGAGGAGG CGGCGCGGAG GAAGGAGCTG GCGGCCAAGT TCGAGGGGCT GGGTGTTGAC
    CTCTTTGGAG AATGCTACCG CAGCAGCGAG GAACGGTCCG CCCGCCTGCT CCTTCGGCGC
    TGCCCACTCT GGGGTGATGC CACTTGCCTG CAGCTCGCCA CACAGGCGGA TGCCCGTGCC
    TTCTTTGCCC AGGATGGGGT ACAGTCTCTG CTGACACAGA AGTGGTGGGG GGAGATGGAC
    AGCACCACGC CCATCTGGGC CCTGGTTCTT GCCTTCTTTT GCCCCCCACT CATCTACACC
    GACCTCATCA CCTTCAGGAA GTCAGATGAG GAGCCCACGC AGAAGGACCC GATGCTTGAC
    GTGGATGGCG ACATCAATGG GGAAGGGCCT GTCGAGGCTA CAGAGCCCCC TGAGAAGATG
    CCCTTGGGGG TACTGAGGCC GCCCAGACAC AGGGACTGCT GTGGGGGGTG CCTGCCGCGC
    CTGCGCCGCT GGCCGCAGTT CTGGGGGGCG CCGGTGACGG CCTTCATGGG CAACGTGGTC
    AGCTACCTCC TCTTCCTGCT GCTGTTTGCG CGCGTGCTGC TCATCGACTT CGAGCCGGAG
    AGGCCCAGCG TGCTGGAGCT GCTGCTCTAC TTCTGGGCCT TCACCCTGCT CTGCGAGGAG
    TTCCGCCAGG GCCTGGGCAG CGGCCTGGGC AGCCTGGCCA TGGGGGGACC CGGGACAGAC
    TCCCACCGGG CCCCACTGCG CCGTCGTCTG CAACTCTACC TCTCTGACGC CTGGAACCAG
    TGCGACCTGG TGGCCCTCGC CTGCTTCGTT CTGGGCGTGG GCTGCAGGCT GACCCCGGGC
    CTGTATGACC TGGGCCGCAC TGTCCTCTGC CTTGACTTCA TGATCTTCAC GCTGAGGCTG
    CTGCACATCT TCACAGTCAA CAAACAGCTG GGGCCCAAGA TCGTCATCGT GAGCAAGATG
    GTGAAGGATG TGTTCTTCTT CCTCTTCTTC CTCGGCGTGT GGCTGGTTGC CTACGGGGTG
    GCCACTGAGG GGCTCCTTAG GCCCCAGGAC CGTAGCCTCC CGAATATCCT GCGCCGTGTC
    TTCTACCGGC CCTACCTGCA GATCTTTGGG CAGATCCCTC AGGGGGAGAT GGACGTGGCC
    CTCATGGAGC ACGGCAACTG TTCACTGGGG CAGGGCTCAT GGGGGCACCC CGAAGGGAAC
    CTCTCTGGCA AATGCGTCTC GCTTTATGCC AACTGGCTGG TGGTGCTCCT CCTTGTTGTC
    TTCCTGCTGG TGGCCAACAT CCTGCTGCTC AATCTGCTCA TTGCGATGTT CAGCCATACC
    TTCGGCAAAG TGCAGGGCAA CAGCGACCTC TACTGGAAGG CGCAGCGCTA CAGCCTCATC
    CGGGAATTTC ACTCTCGGCC CGCACTGGCC CCGCCTCTCA TTTTCATATC GCACGTGCGC
    TCCCTCATCC ATCGATTTCG CAGGCGCCGG GCGCGCTTGC CACCATCCCC AGACGTCGAG
    CATTTTCGGG TCCACCTCTC GAAGGAAGCG GAGCGGAGGC TGTTGACGTG GGAGTCAGTG
    CAAAAGGAGA ATTTCTTGCT GGCGCGTGCT CGGGACAAAC GGGAGAGCAA CTCTGAACGT
    CTGAAGCGCA CGTCGCAGAA GGTGGACACA GCACTGAAGC AGCTGACACA GATCCGCGAG
    TACGAGCAGC GTCTGAAAGG GCTGGAACTG GAGGTCCAGC ACTGTACCCG CGTCCTGGGC
    TGGGTAGCTG AGGCCCTGAG CCGTTCTGCA TTGCTTCCCC CAGGGGGACC ACCCCCCCCA
    ATCCCACCTG GGCCCAAAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59313
    Clone ID Related Accession (Same CDS sequence) XM_010815213.2 , XM_010823820.2 , XM_010815213.3
    Accession Version XM_010815213.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGAT AGAGGATGCC ACCCAGGCTC AGCTCCCCTG CCTCCTGGTG
    GCTGGATCTG GGGGAGCTGC GGACTGCCTG GCAGAGATCC TGGAGGACAC TCTGGCTCCA
    GGGAGTGGCG GGGGCAGGCG ACGTGAACCC CGAGATCTGA TTAAGCGTTT CTTCCCCAAA
    GGGGACCCTG AGGTCCTGCA GGCCCAGGTG GAGAGGATCA TGACTCGGAA GGAGCTGCTG
    ACGGTCTATT CAACTGATGA CGGCCCTGAG GAATTTGAGA CCATCGTCTT AAGGGCCCTT
    GTCAAGGCCT GTGGGAGCTC TGAAGCCTCA GCTTACCTGG ACGAGTTGCG TTTGGCTGTG
    GCTTGGAACC GTGTGGACAT TGCCCAGAGT GAACTCTTCC GGGGGGACAT CCAATGGCGG
    TCCTTCCACC TGGAGGCCTC CCTCATGGAC GCCCTCCTGA ACGACCGACC GGAGTTCGTG
    CGCTTGCTTA TCTCCCATGG CCTCAGCCTG GGCCATTTCC TGACTCCAGT GCGCCTGGCC
    CAGCTCTACA ACGCAGCGCC CCCCAACTCA CTTATCCACA ACCTTCTGGA CCAAGTGTCA
    CACGGTGCAA GCACCAAAGC CCCAGCCCTT AAACCCTCTG AGGAGCCACA GTTTCCTAAG
    GTGGGGCAGG TGCTGCGCAT GCTGCTGGGG AAGACGTGCG CGCCGACGTT CCCCGCCGGG
    GGCACCCGCC GGAGCGACAA CAGCAGAGAG AATTCGTTTA TGCTGTCAGA CAAGGCTGCC
    TCTGGACTGA TGGAAGCTGT CCCCGGGCAG GCCCCCTGGA GCGACCTGCT CCTCTGGGCA
    CTGCTGCTCA ACAGGGCTCA GATGGCCTTG TACTTCTGGG AGATGGGTTC CAATTCTGTG
    GCCTCAGCTC TTGGGGCCTC TTTGTTGCTG CGAGTGTTGG GGCGCCTGGA GACTGAGGCC
    GAGGAGGCGG CGCGGAGGAA GGAGCTGGCG GCCAAGTTCG AGGGGCTGGG TGTTGACCTC
    TTTGGAGAAT GCTACCGCAG CAGCGAGGAA CGGTCCGCCC GCCTGCTCCT TCGGCGCTGC
    CCACTCTGGG GTGATGCCAC TTGCCTGCAG CTCGCCACAC AGGCGGATGC CCGTGCCTTC
    TTTGCCCAGG ATGGGGTACA GTCTCTGCTG ACACAGAAGT GGTGGGGGGA GATGGACAGC
    ACCACGCCCA TCTGGGCCCT GGTTCTTGCC TTCTTTTGCC CCCCACTCAT CTACACCGAC
    CTCATCACCT TCAGGAAGTC AGATGAGGAG CCCACGCAGA AGGACCCGAT GCTTGACGTG
    GATGGCGACA TCAATGGGGA AGGGCCTGTC GAGGCTACAG AGCCCCCTGA GAAGATGCCC
    TTGGGGGTAC TGAGGCCGCC CAGACACAGG GACTGCTGTG GGGGGTGCCT GCCGCGCCTG
    CGCCGCTGGC CGCAGTTCTG GGGGGCGCCG GTGACGGCCT TCATGGGCAA CGTGGTCAGC
    TACCTCCTCT TCCTGCTGCT GTTTGCGCGC GTGCTGCTCA TCGACTTCGA GCCGGAGAGG
    CCCAGCGTGC TGGAGCTGCT GCTCTACTTC TGGGCCTTCA CCCTGCTCTG CGAGGAGTTC
    CGCCAGGGCC TGGGCAGCGG CCTGGGCAGC CTGGCCATGG GGGGACCCGG GACAGACTCC
    CACCGGGCCC CACTGCGCCG TCGTCTGCAA CTCTACCTCT CTGACGCCTG GAACCAGTGC
    GACCTGGTGG CCCTCGCCTG CTTCGTTCTG GGCGTGGGCT GCAGGCTGAC CCCGGGCCTG
    TATGACCTGG GCCGCACTGT CCTCTGCCTT GACTTCATGA TCTTCACGCT GAGGCTGCTG
    CACATCTTCA CAGTCAACAA ACAGCTGGGG CCCAAGATCG TCATCGTGAG CAAGATGGTG
    AAGGATGTGT TCTTCTTCCT CTTCTTCCTC GGCGTGTGGC TGGTTGCCTA CGGGGTGGCC
    ACTGAGGGGC TCCTTAGGCC CCAGGACCGT AGCCTCCCGA ATATCCTGCG CCGTGTCTTC
    TACCGGCCCT ACCTGCAGAT CTTTGGGCAG ATCCCTCAGG GGGAGATGGA CGTGGCCCTC
    ATGGAGCACG GCAACTGTTC ACTGGGGCAG GGCTCATGGG GGCACCCCGA AGGGAACCTC
    TCTGGCAAAT GCGTCTCGCT TTATGCCAAC TGGCTGGTGG TGCTCCTCCT TGTTGTCTTC
    CTGCTGGTGG CCAACATCCT GCTGCTCAAT CTGCTCATTG CGATGTTCAG CCATACCTTC
    GGCAAAGTGC AGGGCAACAG CGACCTCTAC TGGAAGGCGC AGCGCTACAG CCTCATCCGG
    GAATTTCACT CTCGGCCCGC ACTGGCCCCG CCTCTCATTT TCATATCGCA CGTGCGCTCC
    CTCATCCATC GATTTCGCAG GCGCCGGGCG CGCTTGCCAC CATCCCCAGA CGTCGAGCAT
    TTTCGGGTCC ACCTCTCGAA GGAAGCGGAG CGGAGGCTGT TGACGTGGGA GTCAGTGCAA
    AAGGAGAATT TCTTGCTGGC GCGTGCTCGG GACAAACGGG AGAGCAACTC TGAACGTCTG
    AAGCGCACGT CGCAGAAGGT GGACACAGCA CTGAAGCAGC TGACACAGAT CCGCGAGTAC
    GAGCAGCGTC TGAAAGGGCT GGAACTGGAG GTCCAGCACT GTACCCGCGT CCTGGGCTGG
    GTAGCTGAGG CCCTGAGCCG TTCTGCATTG CTTCCCCCAG GGGGACCACC CCCCCCAATC
    CCACCTGGGC CCAAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813515.1
    CDS110..3730
    Translation

    Target ORF information:

    RefSeq Version XM_010815213.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815213.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGAT AGAGGATGCC ACCCAGGCTC AGCTCCCCTG CCTCCTGGTG
    GCTGGATCTG GGGGAGCTGC GGACTGCCTG GCAGAGATCC TGGAGGACAC TCTGGCTCCA
    GGGAGTGGCG GGGGCAGGCG ACGTGAACCC CGAGATCTGA TTAAGCGTTT CTTCCCCAAA
    GGGGACCCTG AGGTCCTGCA GGCCCAGGTG GAGAGGATCA TGACTCGGAA GGAGCTGCTG
    ACGGTCTATT CAACTGATGA CGGCCCTGAG GAATTTGAGA CCATCGTCTT AAGGGCCCTT
    GTCAAGGCCT GTGGGAGCTC TGAAGCCTCA GCTTACCTGG ACGAGTTGCG TTTGGCTGTG
    GCTTGGAACC GTGTGGACAT TGCCCAGAGT GAACTCTTCC GGGGGGACAT CCAATGGCGG
    TCCTTCCACC TGGAGGCCTC CCTCATGGAC GCCCTCCTGA ACGACCGACC GGAGTTCGTG
    CGCTTGCTTA TCTCCCATGG CCTCAGCCTG GGCCATTTCC TGACTCCAGT GCGCCTGGCC
    CAGCTCTACA ACGCAGCGCC CCCCAACTCA CTTATCCACA ACCTTCTGGA CCAAGTGTCA
    CACGGTGCAA GCACCAAAGC CCCAGCCCTT AAACCCTCTG AGGAGCCACA GTTTCCTAAG
    GTGGGGCAGG TGCTGCGCAT GCTGCTGGGG AAGACGTGCG CGCCGACGTT CCCCGCCGGG
    GGCACCCGCC GGAGCGACAA CAGCAGAGAG AATTCGTTTA TGCTGTCAGA CAAGGCTGCC
    TCTGGACTGA TGGAAGCTGT CCCCGGGCAG GCCCCCTGGA GCGACCTGCT CCTCTGGGCA
    CTGCTGCTCA ACAGGGCTCA GATGGCCTTG TACTTCTGGG AGATGGGTTC CAATTCTGTG
    GCCTCAGCTC TTGGGGCCTC TTTGTTGCTG CGAGTGTTGG GGCGCCTGGA GACTGAGGCC
    GAGGAGGCGG CGCGGAGGAA GGAGCTGGCG GCCAAGTTCG AGGGGCTGGG TGTTGACCTC
    TTTGGAGAAT GCTACCGCAG CAGCGAGGAA CGGTCCGCCC GCCTGCTCCT TCGGCGCTGC
    CCACTCTGGG GTGATGCCAC TTGCCTGCAG CTCGCCACAC AGGCGGATGC CCGTGCCTTC
    TTTGCCCAGG ATGGGGTACA GTCTCTGCTG ACACAGAAGT GGTGGGGGGA GATGGACAGC
    ACCACGCCCA TCTGGGCCCT GGTTCTTGCC TTCTTTTGCC CCCCACTCAT CTACACCGAC
    CTCATCACCT TCAGGAAGTC AGATGAGGAG CCCACGCAGA AGGACCCGAT GCTTGACGTG
    GATGGCGACA TCAATGGGGA AGGGCCTGTC GAGGCTACAG AGCCCCCTGA GAAGATGCCC
    TTGGGGGTAC TGAGGCCGCC CAGACACAGG GACTGCTGTG GGGGGTGCCT GCCGCGCCTG
    CGCCGCTGGC CGCAGTTCTG GGGGGCGCCG GTGACGGCCT TCATGGGCAA CGTGGTCAGC
    TACCTCCTCT TCCTGCTGCT GTTTGCGCGC GTGCTGCTCA TCGACTTCGA GCCGGAGAGG
    CCCAGCGTGC TGGAGCTGCT GCTCTACTTC TGGGCCTTCA CCCTGCTCTG CGAGGAGTTC
    CGCCAGGGCC TGGGCAGCGG CCTGGGCAGC CTGGCCATGG GGGGACCCGG GACAGACTCC
    CACCGGGCCC CACTGCGCCG TCGTCTGCAA CTCTACCTCT CTGACGCCTG GAACCAGTGC
    GACCTGGTGG CCCTCGCCTG CTTCGTTCTG GGCGTGGGCT GCAGGCTGAC CCCGGGCCTG
    TATGACCTGG GCCGCACTGT CCTCTGCCTT GACTTCATGA TCTTCACGCT GAGGCTGCTG
    CACATCTTCA CAGTCAACAA ACAGCTGGGG CCCAAGATCG TCATCGTGAG CAAGATGGTG
    AAGGATGTGT TCTTCTTCCT CTTCTTCCTC GGCGTGTGGC TGGTTGCCTA CGGGGTGGCC
    ACTGAGGGGC TCCTTAGGCC CCAGGACCGT AGCCTCCCGA ATATCCTGCG CCGTGTCTTC
    TACCGGCCCT ACCTGCAGAT CTTTGGGCAG ATCCCTCAGG GGGAGATGGA CGTGGCCCTC
    ATGGAGCACG GCAACTGTTC ACTGGGGCAG GGCTCATGGG GGCACCCCGA AGGGAACCTC
    TCTGGCAAAT GCGTCTCGCT TTATGCCAAC TGGCTGGTGG TGCTCCTCCT TGTTGTCTTC
    CTGCTGGTGG CCAACATCCT GCTGCTCAAT CTGCTCATTG CGATGTTCAG CCATACCTTC
    GGCAAAGTGC AGGGCAACAG CGACCTCTAC TGGAAGGCGC AGCGCTACAG CCTCATCCGG
    GAATTTCACT CTCGGCCCGC ACTGGCCCCG CCTCTCATTT TCATATCGCA CGTGCGCTCC
    CTCATCCATC GATTTCGCAG GCGCCGGGCG CGCTTGCCAC CATCCCCAGA CGTCGAGCAT
    TTTCGGGTCC ACCTCTCGAA GGAAGCGGAG CGGAGGCTGT TGACGTGGGA GTCAGTGCAA
    AAGGAGAATT TCTTGCTGGC GCGTGCTCGG GACAAACGGG AGAGCAACTC TGAACGTCTG
    AAGCGCACGT CGCAGAAGGT GGACACAGCA CTGAAGCAGC TGACACAGAT CCGCGAGTAC
    GAGCAGCGTC TGAAAGGGCT GGAACTGGAG GTCCAGCACT GTACCCGCGT CCTGGGCTGG
    GTAGCTGAGG CCCTGAGCCG TTCTGCATTG CTTCCCCCAG GGGGACCACC CCCCCCAATC
    CCACCTGGGC CCAAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59313
    Clone ID Related Accession (Same CDS sequence) XM_010815213.2 , XM_010823820.2 , XM_010815213.3
    Accession Version XM_010823820.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGAT AGAGGATGCC ACCCAGGCTC AGCTCCCCTG CCTCCTGGTG
    GCTGGATCTG GGGGAGCTGC GGACTGCCTG GCAGAGATCC TGGAGGACAC TCTGGCTCCA
    GGGAGTGGCG GGGGCAGGCG ACGTGAACCC CGAGATCTGA TTAAGCGTTT CTTCCCCAAA
    GGGGACCCTG AGGTCCTGCA GGCCCAGGTG GAGAGGATCA TGACTCGGAA GGAGCTGCTG
    ACGGTCTATT CAACTGATGA CGGCCCTGAG GAATTTGAGA CCATCGTCTT AAGGGCCCTT
    GTCAAGGCCT GTGGGAGCTC TGAAGCCTCA GCTTACCTGG ACGAGTTGCG TTTGGCTGTG
    GCTTGGAACC GTGTGGACAT TGCCCAGAGT GAACTCTTCC GGGGGGACAT CCAATGGCGG
    TCCTTCCACC TGGAGGCCTC CCTCATGGAC GCCCTCCTGA ACGACCGACC GGAGTTCGTG
    CGCTTGCTTA TCTCCCATGG CCTCAGCCTG GGCCATTTCC TGACTCCAGT GCGCCTGGCC
    CAGCTCTACA ACGCAGCGCC CCCCAACTCA CTTATCCACA ACCTTCTGGA CCAAGTGTCA
    CACGGTGCAA GCACCAAAGC CCCAGCCCTT AAACCCTCTG AGGAGCCACA GTTTCCTAAG
    GTGGGGCAGG TGCTGCGCAT GCTGCTGGGG AAGACGTGCG CGCCGACGTT CCCCGCCGGG
    GGCACCCGCC GGAGCGACAA CAGCAGAGAG AATTCGTTTA TGCTGTCAGA CAAGGCTGCC
    TCTGGACTGA TGGAAGCTGT CCCCGGGCAG GCCCCCTGGA GCGACCTGCT CCTCTGGGCA
    CTGCTGCTCA ACAGGGCTCA GATGGCCTTG TACTTCTGGG AGATGGGTTC CAATTCTGTG
    GCCTCAGCTC TTGGGGCCTC TTTGTTGCTG CGAGTGTTGG GGCGCCTGGA GACTGAGGCC
    GAGGAGGCGG CGCGGAGGAA GGAGCTGGCG GCCAAGTTCG AGGGGCTGGG TGTTGACCTC
    TTTGGAGAAT GCTACCGCAG CAGCGAGGAA CGGTCCGCCC GCCTGCTCCT TCGGCGCTGC
    CCACTCTGGG GTGATGCCAC TTGCCTGCAG CTCGCCACAC AGGCGGATGC CCGTGCCTTC
    TTTGCCCAGG ATGGGGTACA GTCTCTGCTG ACACAGAAGT GGTGGGGGGA GATGGACAGC
    ACCACGCCCA TCTGGGCCCT GGTTCTTGCC TTCTTTTGCC CCCCACTCAT CTACACCGAC
    CTCATCACCT TCAGGAAGTC AGATGAGGAG CCCACGCAGA AGGACCCGAT GCTTGACGTG
    GATGGCGACA TCAATGGGGA AGGGCCTGTC GAGGCTACAG AGCCCCCTGA GAAGATGCCC
    TTGGGGGTAC TGAGGCCGCC CAGACACAGG GACTGCTGTG GGGGGTGCCT GCCGCGCCTG
    CGCCGCTGGC CGCAGTTCTG GGGGGCGCCG GTGACGGCCT TCATGGGCAA CGTGGTCAGC
    TACCTCCTCT TCCTGCTGCT GTTTGCGCGC GTGCTGCTCA TCGACTTCGA GCCGGAGAGG
    CCCAGCGTGC TGGAGCTGCT GCTCTACTTC TGGGCCTTCA CCCTGCTCTG CGAGGAGTTC
    CGCCAGGGCC TGGGCAGCGG CCTGGGCAGC CTGGCCATGG GGGGACCCGG GACAGACTCC
    CACCGGGCCC CACTGCGCCG TCGTCTGCAA CTCTACCTCT CTGACGCCTG GAACCAGTGC
    GACCTGGTGG CCCTCGCCTG CTTCGTTCTG GGCGTGGGCT GCAGGCTGAC CCCGGGCCTG
    TATGACCTGG GCCGCACTGT CCTCTGCCTT GACTTCATGA TCTTCACGCT GAGGCTGCTG
    CACATCTTCA CAGTCAACAA ACAGCTGGGG CCCAAGATCG TCATCGTGAG CAAGATGGTG
    AAGGATGTGT TCTTCTTCCT CTTCTTCCTC GGCGTGTGGC TGGTTGCCTA CGGGGTGGCC
    ACTGAGGGGC TCCTTAGGCC CCAGGACCGT AGCCTCCCGA ATATCCTGCG CCGTGTCTTC
    TACCGGCCCT ACCTGCAGAT CTTTGGGCAG ATCCCTCAGG GGGAGATGGA CGTGGCCCTC
    ATGGAGCACG GCAACTGTTC ACTGGGGCAG GGCTCATGGG GGCACCCCGA AGGGAACCTC
    TCTGGCAAAT GCGTCTCGCT TTATGCCAAC TGGCTGGTGG TGCTCCTCCT TGTTGTCTTC
    CTGCTGGTGG CCAACATCCT GCTGCTCAAT CTGCTCATTG CGATGTTCAG CCATACCTTC
    GGCAAAGTGC AGGGCAACAG CGACCTCTAC TGGAAGGCGC AGCGCTACAG CCTCATCCGG
    GAATTTCACT CTCGGCCCGC ACTGGCCCCG CCTCTCATTT TCATATCGCA CGTGCGCTCC
    CTCATCCATC GATTTCGCAG GCGCCGGGCG CGCTTGCCAC CATCCCCAGA CGTCGAGCAT
    TTTCGGGTCC ACCTCTCGAA GGAAGCGGAG CGGAGGCTGT TGACGTGGGA GTCAGTGCAA
    AAGGAGAATT TCTTGCTGGC GCGTGCTCGG GACAAACGGG AGAGCAACTC TGAACGTCTG
    AAGCGCACGT CGCAGAAGGT GGACACAGCA CTGAAGCAGC TGACACAGAT CCGCGAGTAC
    GAGCAGCGTC TGAAAGGGCT GGAACTGGAG GTCCAGCACT GTACCCGCGT CCTGGGCTGG
    GTAGCTGAGG CCCTGAGCCG TTCTGCATTG CTTCCCCCAG GGGGACCACC CCCCCCAATC
    CCACCTGGGC CCAAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822122.1
    CDS110..3730
    Translation

    Target ORF information:

    RefSeq Version XM_010823820.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 4 (TRPM4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823820.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGAT AGAGGATGCC ACCCAGGCTC AGCTCCCCTG CCTCCTGGTG
    GCTGGATCTG GGGGAGCTGC GGACTGCCTG GCAGAGATCC TGGAGGACAC TCTGGCTCCA
    GGGAGTGGCG GGGGCAGGCG ACGTGAACCC CGAGATCTGA TTAAGCGTTT CTTCCCCAAA
    GGGGACCCTG AGGTCCTGCA GGCCCAGGTG GAGAGGATCA TGACTCGGAA GGAGCTGCTG
    ACGGTCTATT CAACTGATGA CGGCCCTGAG GAATTTGAGA CCATCGTCTT AAGGGCCCTT
    GTCAAGGCCT GTGGGAGCTC TGAAGCCTCA GCTTACCTGG ACGAGTTGCG TTTGGCTGTG
    GCTTGGAACC GTGTGGACAT TGCCCAGAGT GAACTCTTCC GGGGGGACAT CCAATGGCGG
    TCCTTCCACC TGGAGGCCTC CCTCATGGAC GCCCTCCTGA ACGACCGACC GGAGTTCGTG
    CGCTTGCTTA TCTCCCATGG CCTCAGCCTG GGCCATTTCC TGACTCCAGT GCGCCTGGCC
    CAGCTCTACA ACGCAGCGCC CCCCAACTCA CTTATCCACA ACCTTCTGGA CCAAGTGTCA
    CACGGTGCAA GCACCAAAGC CCCAGCCCTT AAACCCTCTG AGGAGCCACA GTTTCCTAAG
    GTGGGGCAGG TGCTGCGCAT GCTGCTGGGG AAGACGTGCG CGCCGACGTT CCCCGCCGGG
    GGCACCCGCC GGAGCGACAA CAGCAGAGAG AATTCGTTTA TGCTGTCAGA CAAGGCTGCC
    TCTGGACTGA TGGAAGCTGT CCCCGGGCAG GCCCCCTGGA GCGACCTGCT CCTCTGGGCA
    CTGCTGCTCA ACAGGGCTCA GATGGCCTTG TACTTCTGGG AGATGGGTTC CAATTCTGTG
    GCCTCAGCTC TTGGGGCCTC TTTGTTGCTG CGAGTGTTGG GGCGCCTGGA GACTGAGGCC
    GAGGAGGCGG CGCGGAGGAA GGAGCTGGCG GCCAAGTTCG AGGGGCTGGG TGTTGACCTC
    TTTGGAGAAT GCTACCGCAG CAGCGAGGAA CGGTCCGCCC GCCTGCTCCT TCGGCGCTGC
    CCACTCTGGG GTGATGCCAC TTGCCTGCAG CTCGCCACAC AGGCGGATGC CCGTGCCTTC
    TTTGCCCAGG ATGGGGTACA GTCTCTGCTG ACACAGAAGT GGTGGGGGGA GATGGACAGC
    ACCACGCCCA TCTGGGCCCT GGTTCTTGCC TTCTTTTGCC CCCCACTCAT CTACACCGAC
    CTCATCACCT TCAGGAAGTC AGATGAGGAG CCCACGCAGA AGGACCCGAT GCTTGACGTG
    GATGGCGACA TCAATGGGGA AGGGCCTGTC GAGGCTACAG AGCCCCCTGA GAAGATGCCC
    TTGGGGGTAC TGAGGCCGCC CAGACACAGG GACTGCTGTG GGGGGTGCCT GCCGCGCCTG
    CGCCGCTGGC CGCAGTTCTG GGGGGCGCCG GTGACGGCCT TCATGGGCAA CGTGGTCAGC
    TACCTCCTCT TCCTGCTGCT GTTTGCGCGC GTGCTGCTCA TCGACTTCGA GCCGGAGAGG
    CCCAGCGTGC TGGAGCTGCT GCTCTACTTC TGGGCCTTCA CCCTGCTCTG CGAGGAGTTC
    CGCCAGGGCC TGGGCAGCGG CCTGGGCAGC CTGGCCATGG GGGGACCCGG GACAGACTCC
    CACCGGGCCC CACTGCGCCG TCGTCTGCAA CTCTACCTCT CTGACGCCTG GAACCAGTGC
    GACCTGGTGG CCCTCGCCTG CTTCGTTCTG GGCGTGGGCT GCAGGCTGAC CCCGGGCCTG
    TATGACCTGG GCCGCACTGT CCTCTGCCTT GACTTCATGA TCTTCACGCT GAGGCTGCTG
    CACATCTTCA CAGTCAACAA ACAGCTGGGG CCCAAGATCG TCATCGTGAG CAAGATGGTG
    AAGGATGTGT TCTTCTTCCT CTTCTTCCTC GGCGTGTGGC TGGTTGCCTA CGGGGTGGCC
    ACTGAGGGGC TCCTTAGGCC CCAGGACCGT AGCCTCCCGA ATATCCTGCG CCGTGTCTTC
    TACCGGCCCT ACCTGCAGAT CTTTGGGCAG ATCCCTCAGG GGGAGATGGA CGTGGCCCTC
    ATGGAGCACG GCAACTGTTC ACTGGGGCAG GGCTCATGGG GGCACCCCGA AGGGAACCTC
    TCTGGCAAAT GCGTCTCGCT TTATGCCAAC TGGCTGGTGG TGCTCCTCCT TGTTGTCTTC
    CTGCTGGTGG CCAACATCCT GCTGCTCAAT CTGCTCATTG CGATGTTCAG CCATACCTTC
    GGCAAAGTGC AGGGCAACAG CGACCTCTAC TGGAAGGCGC AGCGCTACAG CCTCATCCGG
    GAATTTCACT CTCGGCCCGC ACTGGCCCCG CCTCTCATTT TCATATCGCA CGTGCGCTCC
    CTCATCCATC GATTTCGCAG GCGCCGGGCG CGCTTGCCAC CATCCCCAGA CGTCGAGCAT
    TTTCGGGTCC ACCTCTCGAA GGAAGCGGAG CGGAGGCTGT TGACGTGGGA GTCAGTGCAA
    AAGGAGAATT TCTTGCTGGC GCGTGCTCGG GACAAACGGG AGAGCAACTC TGAACGTCTG
    AAGCGCACGT CGCAGAAGGT GGACACAGCA CTGAAGCAGC TGACACAGAT CCGCGAGTAC
    GAGCAGCGTC TGAAAGGGCT GGAACTGGAG GTCCAGCACT GTACCCGCGT CCTGGGCTGG
    GTAGCTGAGG CCCTGAGCCG TTCTGCATTG CTTCCCCCAG GGGGACCACC CCCCCCAATC
    CCACCTGGGC CCAAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59313
    Clone ID Related Accession (Same CDS sequence) XM_010815213.2 , XM_010823820.2 , XM_010815213.3
    Accession Version XM_010815213.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGAT AGAGGATGCC ACCCAGGCTC AGCTCCCCTG CCTCCTGGTG
    GCTGGATCTG GGGGAGCTGC GGACTGCCTG GCAGAGATCC TGGAGGACAC TCTGGCTCCA
    GGGAGTGGCG GGGGCAGGCG ACGTGAACCC CGAGATCTGA TTAAGCGTTT CTTCCCCAAA
    GGGGACCCTG AGGTCCTGCA GGCCCAGGTG GAGAGGATCA TGACTCGGAA GGAGCTGCTG
    ACGGTCTATT CAACTGATGA CGGCCCTGAG GAATTTGAGA CCATCGTCTT AAGGGCCCTT
    GTCAAGGCCT GTGGGAGCTC TGAAGCCTCA GCTTACCTGG ACGAGTTGCG TTTGGCTGTG
    GCTTGGAACC GTGTGGACAT TGCCCAGAGT GAACTCTTCC GGGGGGACAT CCAATGGCGG
    TCCTTCCACC TGGAGGCCTC CCTCATGGAC GCCCTCCTGA ACGACCGACC GGAGTTCGTG
    CGCTTGCTTA TCTCCCATGG CCTCAGCCTG GGCCATTTCC TGACTCCAGT GCGCCTGGCC
    CAGCTCTACA ACGCAGCGCC CCCCAACTCA CTTATCCACA ACCTTCTGGA CCAAGTGTCA
    CACGGTGCAA GCACCAAAGC CCCAGCCCTT AAACCCTCTG AGGAGCCACA GTTTCCTAAG
    GTGGGGCAGG TGCTGCGCAT GCTGCTGGGG AAGACGTGCG CGCCGACGTT CCCCGCCGGG
    GGCACCCGCC GGAGCGACAA CAGCAGAGAG AATTCGTTTA TGCTGTCAGA CAAGGCTGCC
    TCTGGACTGA TGGAAGCTGT CCCCGGGCAG GCCCCCTGGA GCGACCTGCT CCTCTGGGCA
    CTGCTGCTCA ACAGGGCTCA GATGGCCTTG TACTTCTGGG AGATGGGTTC CAATTCTGTG
    GCCTCAGCTC TTGGGGCCTC TTTGTTGCTG CGAGTGTTGG GGCGCCTGGA GACTGAGGCC
    GAGGAGGCGG CGCGGAGGAA GGAGCTGGCG GCCAAGTTCG AGGGGCTGGG TGTTGACCTC
    TTTGGAGAAT GCTACCGCAG CAGCGAGGAA CGGTCCGCCC GCCTGCTCCT TCGGCGCTGC
    CCACTCTGGG GTGATGCCAC TTGCCTGCAG CTCGCCACAC AGGCGGATGC CCGTGCCTTC
    TTTGCCCAGG ATGGGGTACA GTCTCTGCTG ACACAGAAGT GGTGGGGGGA GATGGACAGC
    ACCACGCCCA TCTGGGCCCT GGTTCTTGCC TTCTTTTGCC CCCCACTCAT CTACACCGAC
    CTCATCACCT TCAGGAAGTC AGATGAGGAG CCCACGCAGA AGGACCCGAT GCTTGACGTG
    GATGGCGACA TCAATGGGGA AGGGCCTGTC GAGGCTACAG AGCCCCCTGA GAAGATGCCC
    TTGGGGGTAC TGAGGCCGCC CAGACACAGG GACTGCTGTG GGGGGTGCCT GCCGCGCCTG
    CGCCGCTGGC CGCAGTTCTG GGGGGCGCCG GTGACGGCCT TCATGGGCAA CGTGGTCAGC
    TACCTCCTCT TCCTGCTGCT GTTTGCGCGC GTGCTGCTCA TCGACTTCGA GCCGGAGAGG
    CCCAGCGTGC TGGAGCTGCT GCTCTACTTC TGGGCCTTCA CCCTGCTCTG CGAGGAGTTC
    CGCCAGGGCC TGGGCAGCGG CCTGGGCAGC CTGGCCATGG GGGGACCCGG GACAGACTCC
    CACCGGGCCC CACTGCGCCG TCGTCTGCAA CTCTACCTCT CTGACGCCTG GAACCAGTGC
    GACCTGGTGG CCCTCGCCTG CTTCGTTCTG GGCGTGGGCT GCAGGCTGAC CCCGGGCCTG
    TATGACCTGG GCCGCACTGT CCTCTGCCTT GACTTCATGA TCTTCACGCT GAGGCTGCTG
    CACATCTTCA CAGTCAACAA ACAGCTGGGG CCCAAGATCG TCATCGTGAG CAAGATGGTG
    AAGGATGTGT TCTTCTTCCT CTTCTTCCTC GGCGTGTGGC TGGTTGCCTA CGGGGTGGCC
    ACTGAGGGGC TCCTTAGGCC CCAGGACCGT AGCCTCCCGA ATATCCTGCG CCGTGTCTTC
    TACCGGCCCT ACCTGCAGAT CTTTGGGCAG ATCCCTCAGG GGGAGATGGA CGTGGCCCTC
    ATGGAGCACG GCAACTGTTC ACTGGGGCAG GGCTCATGGG GGCACCCCGA AGGGAACCTC
    TCTGGCAAAT GCGTCTCGCT TTATGCCAAC TGGCTGGTGG TGCTCCTCCT TGTTGTCTTC
    CTGCTGGTGG CCAACATCCT GCTGCTCAAT CTGCTCATTG CGATGTTCAG CCATACCTTC
    GGCAAAGTGC AGGGCAACAG CGACCTCTAC TGGAAGGCGC AGCGCTACAG CCTCATCCGG
    GAATTTCACT CTCGGCCCGC ACTGGCCCCG CCTCTCATTT TCATATCGCA CGTGCGCTCC
    CTCATCCATC GATTTCGCAG GCGCCGGGCG CGCTTGCCAC CATCCCCAGA CGTCGAGCAT
    TTTCGGGTCC ACCTCTCGAA GGAAGCGGAG CGGAGGCTGT TGACGTGGGA GTCAGTGCAA
    AAGGAGAATT TCTTGCTGGC GCGTGCTCGG GACAAACGGG AGAGCAACTC TGAACGTCTG
    AAGCGCACGT CGCAGAAGGT GGACACAGCA CTGAAGCAGC TGACACAGAT CCGCGAGTAC
    GAGCAGCGTC TGAAAGGGCT GGAACTGGAG GTCCAGCACT GTACCCGCGT CCTGGGCTGG
    GTAGCTGAGG CCCTGAGCCG TTCTGCATTG CTTCCCCCAG GGGGACCACC CCCCCCAATC
    CCACCTGGGC CCAAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813515.1
    CDS128..3748
    Translation

    Target ORF information:

    RefSeq Version XM_010815213.3
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815213.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGTGGGTG CGGAGAAGGA GCAGAGCTGG ATTCCCAAGA TCTTCAAGAA GAAGACGTGC 
    ACGACTTTCG TCGCTGACCC CACAGATTCG GGGGGGAGCT TGTGCCAGTG TGGGCATCCT
    CGGGGTGCCC ACCCATCCGT GGCTGTGGAG GATGCATTCG GGGCAGCCGT GGTGACCGTA
    TGGGACAGTG ACTTGCACAC CACGGAGAAG CCCACAGATG CCTTCGGGGA CCTGGACTTC
    CTGGGCGCCG GCCGCAAGAT CAGCAATTTC CTCCGGCTCT CGGACCGCAC GGATCCAGCT
    ACGGTTTATA CTCTGGTCAC CAAAGTGTGG GGCTTCCGGG CCCCGAACCT GGTGGTGTCA
    GTGCTGGGGG GGTCAGGGGG CCCCATCCTC CAGACCTGGC TGCAGGACCT GCTGCGACGC
    GGGCTGGTGC GGGCTGCCCA GAGCACAGGG GCCTGGATTG TCACTGGAGG GCTGCACAAA
    GGCATCGGCC GGCACGTCGG TGCGGCTGTG CGGGACCACC AGACGGCCAG CACTGGGGGC
    AGCAAAGTGG TGGCCATGGG CGTGGCCCCC TGGGGCGTGG TGCGGAATAG AGACACTCTC
    ACCAACCCCA AGGGCTCGTT CCCCGCAAGG TACCGGTGGC GCGGAGACCC AGAGGATGGG
    GTCCAGTTCC CTCTTGACTA CAACTACTCG GCCTTCTTCC TGGTGGATGA CGGCACACAC
    GGCCGCCTGG GCGGCGAGAA CCGCTTCCGC TTGGGCTTCG AGTCCTACCT GGCCCAACAG
    AAGACGGGCG TGGGAGGGAC TGGCATTGAC ATCCCTGTCC TCCTCCTCCT GATTGATGGT
    GATGAGAAGA TGTTGAAGAT AGAGGATGCC ACCCAGGCTC AGCTCCCCTG CCTCCTGGTG
    GCTGGATCTG GGGGAGCTGC GGACTGCCTG GCAGAGATCC TGGAGGACAC TCTGGCTCCA
    GGGAGTGGCG GGGGCAGGCG ACGTGAACCC CGAGATCTGA TTAAGCGTTT CTTCCCCAAA
    GGGGACCCTG AGGTCCTGCA GGCCCAGGTG GAGAGGATCA TGACTCGGAA GGAGCTGCTG
    ACGGTCTATT CAACTGATGA CGGCCCTGAG GAATTTGAGA CCATCGTCTT AAGGGCCCTT
    GTCAAGGCCT GTGGGAGCTC TGAAGCCTCA GCTTACCTGG ACGAGTTGCG TTTGGCTGTG
    GCTTGGAACC GTGTGGACAT TGCCCAGAGT GAACTCTTCC GGGGGGACAT CCAATGGCGG
    TCCTTCCACC TGGAGGCCTC CCTCATGGAC GCCCTCCTGA ACGACCGACC GGAGTTCGTG
    CGCTTGCTTA TCTCCCATGG CCTCAGCCTG GGCCATTTCC TGACTCCAGT GCGCCTGGCC
    CAGCTCTACA ACGCAGCGCC CCCCAACTCA CTTATCCACA ACCTTCTGGA CCAAGTGTCA
    CACGGTGCAA GCACCAAAGC CCCAGCCCTT AAACCCTCTG AGGAGCCACA GTTTCCTAAG
    GTGGGGCAGG TGCTGCGCAT GCTGCTGGGG AAGACGTGCG CGCCGACGTT CCCCGCCGGG
    GGCACCCGCC GGAGCGACAA CAGCAGAGAG AATTCGTTTA TGCTGTCAGA CAAGGCTGCC
    TCTGGACTGA TGGAAGCTGT CCCCGGGCAG GCCCCCTGGA GCGACCTGCT CCTCTGGGCA
    CTGCTGCTCA ACAGGGCTCA GATGGCCTTG TACTTCTGGG AGATGGGTTC CAATTCTGTG
    GCCTCAGCTC TTGGGGCCTC TTTGTTGCTG CGAGTGTTGG GGCGCCTGGA GACTGAGGCC
    GAGGAGGCGG CGCGGAGGAA GGAGCTGGCG GCCAAGTTCG AGGGGCTGGG TGTTGACCTC
    TTTGGAGAAT GCTACCGCAG CAGCGAGGAA CGGTCCGCCC GCCTGCTCCT TCGGCGCTGC
    CCACTCTGGG GTGATGCCAC TTGCCTGCAG CTCGCCACAC AGGCGGATGC CCGTGCCTTC
    TTTGCCCAGG ATGGGGTACA GTCTCTGCTG ACACAGAAGT GGTGGGGGGA GATGGACAGC
    ACCACGCCCA TCTGGGCCCT GGTTCTTGCC TTCTTTTGCC CCCCACTCAT CTACACCGAC
    CTCATCACCT TCAGGAAGTC AGATGAGGAG CCCACGCAGA AGGACCCGAT GCTTGACGTG
    GATGGCGACA TCAATGGGGA AGGGCCTGTC GAGGCTACAG AGCCCCCTGA GAAGATGCCC
    TTGGGGGTAC TGAGGCCGCC CAGACACAGG GACTGCTGTG GGGGGTGCCT GCCGCGCCTG
    CGCCGCTGGC CGCAGTTCTG GGGGGCGCCG GTGACGGCCT TCATGGGCAA CGTGGTCAGC
    TACCTCCTCT TCCTGCTGCT GTTTGCGCGC GTGCTGCTCA TCGACTTCGA GCCGGAGAGG
    CCCAGCGTGC TGGAGCTGCT GCTCTACTTC TGGGCCTTCA CCCTGCTCTG CGAGGAGTTC
    CGCCAGGGCC TGGGCAGCGG CCTGGGCAGC CTGGCCATGG GGGGACCCGG GACAGACTCC
    CACCGGGCCC CACTGCGCCG TCGTCTGCAA CTCTACCTCT CTGACGCCTG GAACCAGTGC
    GACCTGGTGG CCCTCGCCTG CTTCGTTCTG GGCGTGGGCT GCAGGCTGAC CCCGGGCCTG
    TATGACCTGG GCCGCACTGT CCTCTGCCTT GACTTCATGA TCTTCACGCT GAGGCTGCTG
    CACATCTTCA CAGTCAACAA ACAGCTGGGG CCCAAGATCG TCATCGTGAG CAAGATGGTG
    AAGGATGTGT TCTTCTTCCT CTTCTTCCTC GGCGTGTGGC TGGTTGCCTA CGGGGTGGCC
    ACTGAGGGGC TCCTTAGGCC CCAGGACCGT AGCCTCCCGA ATATCCTGCG CCGTGTCTTC
    TACCGGCCCT ACCTGCAGAT CTTTGGGCAG ATCCCTCAGG GGGAGATGGA CGTGGCCCTC
    ATGGAGCACG GCAACTGTTC ACTGGGGCAG GGCTCATGGG GGCACCCCGA AGGGAACCTC
    TCTGGCAAAT GCGTCTCGCT TTATGCCAAC TGGCTGGTGG TGCTCCTCCT TGTTGTCTTC
    CTGCTGGTGG CCAACATCCT GCTGCTCAAT CTGCTCATTG CGATGTTCAG CCATACCTTC
    GGCAAAGTGC AGGGCAACAG CGACCTCTAC TGGAAGGCGC AGCGCTACAG CCTCATCCGG
    GAATTTCACT CTCGGCCCGC ACTGGCCCCG CCTCTCATTT TCATATCGCA CGTGCGCTCC
    CTCATCCATC GATTTCGCAG GCGCCGGGCG CGCTTGCCAC CATCCCCAGA CGTCGAGCAT
    TTTCGGGTCC ACCTCTCGAA GGAAGCGGAG CGGAGGCTGT TGACGTGGGA GTCAGTGCAA
    AAGGAGAATT TCTTGCTGGC GCGTGCTCGG GACAAACGGG AGAGCAACTC TGAACGTCTG
    AAGCGCACGT CGCAGAAGGT GGACACAGCA CTGAAGCAGC TGACACAGAT CCGCGAGTAC
    GAGCAGCGTC TGAAAGGGCT GGAACTGGAG GTCCAGCACT GTACCCGCGT CCTGGGCTGG
    GTAGCTGAGG CCCTGAGCCG TTCTGCATTG CTTCCCCCAG GGGGACCACC CCCCCCAATC
    CCACCTGGGC CCAAAGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.