USP13 cDNA ORF clone, Bos taurus(cattle)

The following USP13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa44436 NM_001191266.1
Latest version!
Bos taurus ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa59228 XM_010801341.2
Latest version!
Bos taurus ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa216421 NM_001191266.2
Latest version!
Bos taurus ubiquitin specific peptidase 13 (USP13), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa87765 XM_015472368.2
Latest version!
Bos taurus ubiquitin specific peptidase 13 (USP13), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OBa87765 XM_015472368.1 Bos taurus ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa44436
    Clone ID Related Accession (Same CDS sequence) NM_001191266.1
    Accession Version NM_001191266.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product ubiquitin carboxyl-terminal hydrolase 13
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03125215.1, AAFC03025959.1 and AAFC03042984.1. On Jul 17, 2010 this sequence version replaced XM_001787362.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGCGCC GGGGCGCCCT GTTCGGCATG CCGGGCGGTA GCGGCAGCAG GAAGATGGCT 
    GCAGGAGACA TCGGCGAGCT GCTAGTGCCC CACATGCCCA CGATCCGCGT GCCCAGGTCC
    GGGGACAGGG TCTACAAGAA CGAGTGCGCC TTCTCCTACG ACTCCCCGAA TTCTGAAGGC
    GGACTCTATG TCTGCATGAA TACATTTTTG GCCTTTGGAA GGGAACACGT GGAAAGACAC
    TTTCGAAAAA CTGGACAGAG TGTCTACATG CATCTGAAGA GACACGTGCG CGAGAAGGTA
    AGAGGCGCAT CTGGTGGCGC TTTACCCAAA AGGAGGAATT CCAAGATGTT TTTAGATCTA
    GATACTGATG ACGATTTAAA TAGCGACGAT TATGAATATG AAGATGAAGC CAAACTTGTT
    ATATTCCCAG ACCACTATGA AATAGCCCTA CCAAATATTG AGGAGTTACC AGCCCTGGTA
    ACCATTGCTT GTGATGCAGT TCTCAGCTCA AAATCTCCAT ACAGAAAGCA GGACCCAGAT
    ACATGGGAGA ATGAATTGCC AGTGTCGAAA TATGCCAATA ACCTTACCCA GTTGGACAAT
    GGAGTCAGAA TTCCTCCAAG TGGTTGGAAG TGTGCCAGGT GCGACCTTCG GGAGAACCTC
    TGGTTGAACC TGACCGATGG CTCCGTGCTG TGTGGGAAGT GGTTCTTTGA CAGCTCTGGG
    GGCAACGGGC ATGCGTTGGA GCATTACAGA GACACAGGCT ACCCTCTCGC TGTGAAACTG
    GGGACCATCA CTCCGGATGG GGCCGATGTT TATTCTTTCC AAGAAGAGGA AGCTGTTTTG
    GACCCTCATT TGGCCAAGCA CTTAGCACAT TTTGGAATTG ACATGCTTCA TATGCATGGG
    ACAGAGAATG GGCTCCAGGA CAACGACATC AAGCCGCGGG TCAGCGAGTG GGAGGTGATC
    CAGGAGACGG GGACAAAGCT GAAGCCCATG TACGGCCCAG GGTACACAGG GCTCAAGAAC
    CTGGGCAATA GCTGCTATCT CAGCTCTGTG ATGCAGGCCA TCTTCAGCAT CCCAGAATTC
    CAGAGAGCGT ATGTAGGAAA TCTTCCAAGA ATATTTGACT ACTCTCCTTT AGATCCAACA
    CAAGATTTCA ACACACAGAT GACTAAGTTA GGACATGGCC TTCTCTCAGG CCAGTATTCA
    AAACCTCCCG TGAAGTCTGA GCTCATTGAA CAGGTGATGA AGGAGGAACA CAAGCCTCAA
    CAGAATGGGA TCTCTCCACG CATGTTTAAG GCCTTTGTGA GCAAGAGCCA CCCGGAATTC
    TCCTCCAACA GACAGCAGGA TGCCCAGGAG TTTTTCCTGC ACCTGGTGAA TCTAGTAGAG
    AGGAACCGCA TTGGCTCAGA AAACCCAAGT GATGTTTTTC GGTTTTTGGT GGAAGAGCGG
    ATTCAGTGCT GTCAGACCCG AAAAGTCCGC TACACGGAGA GGGTGGACTA CCTGATGCAG
    TTACCTGTGG CCATGGAGGC AGCAACCAAC AAAGATGAAC TGATCGCCTA TGAACTAACG
    AGAAGGGAAG CAGAATCAAA CAGAAGACCC CTTCCCGAGT TGGTTCGTGC CAAGATACCA
    TTCAGTGCCT GCCTGCAGGC CTTTTCTGAA CCAGAAAATG TTGATGATTT CTGGAGCAGC
    GCCTTGCAAG CAAAGTCTGC GGGTGTGAAA ACATCTCGCT TTGCCTCATT CCCTGAATAC
    TTGGTAGTGC AGATAAAGAA GTTCACTTTT GGTCTTGACT GGGTTCCCAA AAAATTTGAT
    GTTTCTGTTG ATATGCCAGA CCTACTTGAT ATCAACCATC TCCGAGCCAG GGGGTTGCAG
    CCAGGAGAGG AGGAACTTCC AGACATCAGC CCCCCCATAG TGATTCCTGA TGACTCTAAA
    GATCGCCTGA TGACCCAATT GATAGACCCA TCAGATATTG ATGAGTCTTC GGTGATGCAG
    CTGGCTGAGA TGGGCTTCCC TCTAGAAGCA TGTCGGAAGG CTGTGTATTT TACTGGAAAT
    ATGGGAGCCG AGGTGGCCTT CAACTGGATC GTTGTTCACA TGGAAGAGCC AGATTTTGCT
    GAACCGCTGA CCATGCCTGG TTATGGAGGA GCAGCTTCTG CTGGGGCCTC TGTTTTTGGT
    GCTACTGGAT TGGATAACCA ACCTCCGGAA GAAACTGTAG CTATCATTAC CTCCATGGGA
    TTCCATCGAA ATCAGGCTAT TCAGGCCCTG CGAGCAACGA ATAGTAACCT GGAAAGAGCA
    CTGGATTGGA TCTTTAGCCA CCCTGAGTTT GAGGAGGACA GTGACTTTGT GATCGAGATG
    GAGAATAACG CCAATGCGAA CATCGTTTCT GAGGCCAAAC CTGAAGGACC TAGAGTCAAG
    GATGGATCTG GAATGTATGA ATTATTTGCG TTCATCAGTC ACATGGGAAC GTCTACAATG
    AGTGGCCACT ACGTTTGTCA CATTAAAAAG GAAGGAAGAT GGGTGATCTA CAATGACCAC
    AAAGTTTGTG CCTCAGAAAG GCCCCCTAAA GACCTGGGCT ACATGTACTT TTACCGCAGG
    ATACCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178195.1
    CDS80..2671
    Translation

    Target ORF information:

    RefSeq Version NM_001191266.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191266.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGCGCC GGGGCGCCCT GTTCGGCATG CCGGGCGGTA GCGGCAGCAG GAAGATGGCT 
    GCAGGAGACA TCGGCGAGCT GCTAGTGCCC CACATGCCCA CGATCCGCGT GCCCAGGTCC
    GGGGACAGGG TCTACAAGAA CGAGTGCGCC TTCTCCTACG ACTCCCCGAA TTCTGAAGGC
    GGACTCTATG TCTGCATGAA TACATTTTTG GCCTTTGGAA GGGAACACGT GGAAAGACAC
    TTTCGAAAAA CTGGACAGAG TGTCTACATG CATCTGAAGA GACACGTGCG CGAGAAGGTA
    AGAGGCGCAT CTGGTGGCGC TTTACCCAAA AGGAGGAATT CCAAGATGTT TTTAGATCTA
    GATACTGATG ACGATTTAAA TAGCGACGAT TATGAATATG AAGATGAAGC CAAACTTGTT
    ATATTCCCAG ACCACTATGA AATAGCCCTA CCAAATATTG AGGAGTTACC AGCCCTGGTA
    ACCATTGCTT GTGATGCAGT TCTCAGCTCA AAATCTCCAT ACAGAAAGCA GGACCCAGAT
    ACATGGGAGA ATGAATTGCC AGTGTCGAAA TATGCCAATA ACCTTACCCA GTTGGACAAT
    GGAGTCAGAA TTCCTCCAAG TGGTTGGAAG TGTGCCAGGT GCGACCTTCG GGAGAACCTC
    TGGTTGAACC TGACCGATGG CTCCGTGCTG TGTGGGAAGT GGTTCTTTGA CAGCTCTGGG
    GGCAACGGGC ATGCGTTGGA GCATTACAGA GACACAGGCT ACCCTCTCGC TGTGAAACTG
    GGGACCATCA CTCCGGATGG GGCCGATGTT TATTCTTTCC AAGAAGAGGA AGCTGTTTTG
    GACCCTCATT TGGCCAAGCA CTTAGCACAT TTTGGAATTG ACATGCTTCA TATGCATGGG
    ACAGAGAATG GGCTCCAGGA CAACGACATC AAGCCGCGGG TCAGCGAGTG GGAGGTGATC
    CAGGAGACGG GGACAAAGCT GAAGCCCATG TACGGCCCAG GGTACACAGG GCTCAAGAAC
    CTGGGCAATA GCTGCTATCT CAGCTCTGTG ATGCAGGCCA TCTTCAGCAT CCCAGAATTC
    CAGAGAGCGT ATGTAGGAAA TCTTCCAAGA ATATTTGACT ACTCTCCTTT AGATCCAACA
    CAAGATTTCA ACACACAGAT GACTAAGTTA GGACATGGCC TTCTCTCAGG CCAGTATTCA
    AAACCTCCCG TGAAGTCTGA GCTCATTGAA CAGGTGATGA AGGAGGAACA CAAGCCTCAA
    CAGAATGGGA TCTCTCCACG CATGTTTAAG GCCTTTGTGA GCAAGAGCCA CCCGGAATTC
    TCCTCCAACA GACAGCAGGA TGCCCAGGAG TTTTTCCTGC ACCTGGTGAA TCTAGTAGAG
    AGGAACCGCA TTGGCTCAGA AAACCCAAGT GATGTTTTTC GGTTTTTGGT GGAAGAGCGG
    ATTCAGTGCT GTCAGACCCG AAAAGTCCGC TACACGGAGA GGGTGGACTA CCTGATGCAG
    TTACCTGTGG CCATGGAGGC AGCAACCAAC AAAGATGAAC TGATCGCCTA TGAACTAACG
    AGAAGGGAAG CAGAATCAAA CAGAAGACCC CTTCCCGAGT TGGTTCGTGC CAAGATACCA
    TTCAGTGCCT GCCTGCAGGC CTTTTCTGAA CCAGAAAATG TTGATGATTT CTGGAGCAGC
    GCCTTGCAAG CAAAGTCTGC GGGTGTGAAA ACATCTCGCT TTGCCTCATT CCCTGAATAC
    TTGGTAGTGC AGATAAAGAA GTTCACTTTT GGTCTTGACT GGGTTCCCAA AAAATTTGAT
    GTTTCTGTTG ATATGCCAGA CCTACTTGAT ATCAACCATC TCCGAGCCAG GGGGTTGCAG
    CCAGGAGAGG AGGAACTTCC AGACATCAGC CCCCCCATAG TGATTCCTGA TGACTCTAAA
    GATCGCCTGA TGACCCAATT GATAGACCCA TCAGATATTG ATGAGTCTTC GGTGATGCAG
    CTGGCTGAGA TGGGCTTCCC TCTAGAAGCA TGTCGGAAGG CTGTGTATTT TACTGGAAAT
    ATGGGAGCCG AGGTGGCCTT CAACTGGATC GTTGTTCACA TGGAAGAGCC AGATTTTGCT
    GAACCGCTGA CCATGCCTGG TTATGGAGGA GCAGCTTCTG CTGGGGCCTC TGTTTTTGGT
    GCTACTGGAT TGGATAACCA ACCTCCGGAA GAAACTGTAG CTATCATTAC CTCCATGGGA
    TTCCATCGAA ATCAGGCTAT TCAGGCCCTG CGAGCAACGA ATAGTAACCT GGAAAGAGCA
    CTGGATTGGA TCTTTAGCCA CCCTGAGTTT GAGGAGGACA GTGACTTTGT GATCGAGATG
    GAGAATAACG CCAATGCGAA CATCGTTTCT GAGGCCAAAC CTGAAGGACC TAGAGTCAAG
    GATGGATCTG GAATGTATGA ATTATTTGCG TTCATCAGTC ACATGGGAAC GTCTACAATG
    AGTGGCCACT ACGTTTGTCA CATTAAAAAG GAAGGAAGAT GGGTGATCTA CAATGACCAC
    AAAGTTTGTG CCTCAGAAAG GCCCCCTAAA GACCTGGGCT ACATGTACTT TTACCGCAGG
    ATACCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59228
    Clone ID Related Accession (Same CDS sequence) XM_010801341.2
    Accession Version XM_010801341.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 13 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103814.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAATACAT TTTTGGCCTT TGGAAGGGAA CACGTGGAAA GACACTTTCG AAAAACTGGA 
    CAGAGTGTCT ACATGCATCT GAAGAGACAC GTGCGCGAGA AGGTAAGAGG CGCATCTGGT
    GGCGCTTTAC CCAAAAGGAG GAATTCCAAG ATGTTTTTAG ATCTAGATAC TGATGACGAT
    TTAAATAGCG ACGATTATGA ATATGAAGAT GAAGCCAAAC TTGTTATATT CCCAGACCAC
    TATGAAATAG CCCTACCAAA TATTGAGGAG TTACCAGCCC TGGTAACCAT TGCTTGTGAT
    GCAGTTCTCA GCTCAAAATC TCCATACAGA AAGCAGGACC CAGATACATG GGAGAATGAA
    TTGCCAGTGT CGAAATATGC CAATAACCTT ACCCAGTTGG ACAATGGAGT CAGAATTCCT
    CCAAGTGGTT GGAAGTGTGC CAGATGCGAC CTTCGGGAGA ACCTCTGGTT GAACCTGACC
    GATGGCTCCG TGCTGTGTGG GAAGTGGTTC TTTGACAGCT CTGGGGGCAA CGGGCATGCG
    TTGGAGCATT ACAGAGACAC AGGCTACCCT CTCGCTGTGA AACTGGGGAC CATCACTCCG
    GATGGGGCCG ATGTTTATTC TTTCCAAGAA GAGGAAGCTG TTTTGGACCC TCATTTGGCC
    AAGCACTTAG CACATTTTGG AATTGACATG CTTCATATGC ATGGGACAGA GAATGGGCTC
    CAGGACAACG ACATCAAGCC GCGGGTCAGC GAGTGGGAGG TGATCCAGGA GACGGGGACA
    AAGCTGAAGC CCATGTACGG CCCAGGGTAC ACAGGGCTCA AGAACCTGGG CAATAGCTGC
    TATCTCAGCT CTGTGATGCA GGCCATCTTC AGCATCCCAG AATTCCAGAG AGCGTATGTA
    GGAAATCTTC CAAGAATATT TGACTACTCT CCTTTAGATC CAACACAAGA TTTCAACACA
    CAGATGACTA AGTTAGGACA TGGCCTTCTC TCAGGCCAGT ATTCAAAACC TCCCGTGAAG
    TCTGAGCTCA TTGAACAGGT GATGAAGGAG GAACACAAGC CTCAACAGAA TGGGATCTCT
    CCACGCATGT TTAAGGCCTT TGTGAGCAAG AGCCACCCGG AATTCTCCTC CAACAGACAG
    CAGGATGCCC AGGAGTTTTT CCTGCACCTG GTGAATCTAG TAGAGAGGAA CCGCATTGGC
    TCAGAAAACC CAAGTGATGT TTTTCGGTTT TTGGTGGAAG AGCGGATTCA GTGCTGTCAG
    ACCCGAAAAG TCCGCTACAC GGAGAGGGTG GACTACCTGA TGCAGTTACC TGTGGCCATG
    GAGGCAGCAA CCAACAAAGA TGAACTGATC GCCTATGAAC TAACGAGAAG GGAAGCAGAA
    TCAAACAGAA GACCCCTTCC CGAGTTGGTT CGTGCCAAGA TACCATTCAG TGCCTGCCTG
    CAGGCCTTTT CTGAACCAGA AAATGTTGAT GATTTCTGGA GCAGCGCCCT GCAAGCAAAG
    TCTGCGGGTG TGAAAACATC TCGCTTTGCC TCATTCCCTG AATACTTGGT AGTGCAGATA
    AAGAAGTTCA CTTTTGGTCT TGACTGGGTT CCCAAAAAAT TTGATGTTTC TGTTGATATG
    CCAGACCTAC TTGATATCAA CCATCTCCGA GCCAGGGGGT TGCAGCCAGG AGAGGAGGAA
    CTTCCAGACA TCAGCCCCCC CATAGTGATT CCTGATGACT CTAAAGATCG CCTGATGACC
    CAATTGATAG ACCCATCAGA TATTGATGAG TCTTCGGTGA TGCAGCTGGC TGAGATGGGC
    TTCCCTCTAG AAGCATGTCG GAAGGCTGTG TATTTTACTG GAAATATGGG AGCCGAGGTG
    GCCTTCAACT GGATCGTTGT TCACATGGAA GAGCCAGATT TTGCTGAACC GCTGACCATG
    CCTGGTTATG GAGGAGCAGC TTCTGCTGGG GCCTCTGTTT TTGGTGCTAC TGGATTGGAT
    AACCAACCTC CGGAAGAAAC TGTAGCTATC ATTACCTCCA TGGGATTCCA TCGAAATCAG
    GCTATTCAGG CCCTGCGAGC AACGAATAGT AACCTGGAAA GAGCACTGGA TTGGATCTTT
    AGCCACCCTG AGTTTGAGGA GGACAGTGAC TTTGTGATCG AGATGGAGAA TAACGCCAAT
    GCGAACATCG TTTCTGAGGC CAAACCTGAA GGACCTAGAG TCAAGGATGG ATCTGGAATG
    TATGAATTAT TTGCGTTCAT CAGTCACATG GGAACGTCTA CAATGAGTGG CCACTACGTT
    TGTCACATTA AAAAGGAAGG AAGATGGGTG ATCTACAATG ACCACAAAGT TTGTGCCTCA
    GAAAGGCCCC CTAAAGACCT GGGCTACATG TACTTTTACC GCAGGATACC AAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799643.1
    CDS153..2549
    Translation

    Target ORF information:

    RefSeq Version XM_010801341.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801341.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAATACAT TTTTGGCCTT TGGAAGGGAA CACGTGGAAA GACACTTTCG AAAAACTGGA 
    CAGAGTGTCT ACATGCATCT GAAGAGACAC GTGCGCGAGA AGGTAAGAGG CGCATCTGGT
    GGCGCTTTAC CCAAAAGGAG GAATTCCAAG ATGTTTTTAG ATCTAGATAC TGATGACGAT
    TTAAATAGCG ACGATTATGA ATATGAAGAT GAAGCCAAAC TTGTTATATT CCCAGACCAC
    TATGAAATAG CCCTACCAAA TATTGAGGAG TTACCAGCCC TGGTAACCAT TGCTTGTGAT
    GCAGTTCTCA GCTCAAAATC TCCATACAGA AAGCAGGACC CAGATACATG GGAGAATGAA
    TTGCCAGTGT CGAAATATGC CAATAACCTT ACCCAGTTGG ACAATGGAGT CAGAATTCCT
    CCAAGTGGTT GGAAGTGTGC CAGATGCGAC CTTCGGGAGA ACCTCTGGTT GAACCTGACC
    GATGGCTCCG TGCTGTGTGG GAAGTGGTTC TTTGACAGCT CTGGGGGCAA CGGGCATGCG
    TTGGAGCATT ACAGAGACAC AGGCTACCCT CTCGCTGTGA AACTGGGGAC CATCACTCCG
    GATGGGGCCG ATGTTTATTC TTTCCAAGAA GAGGAAGCTG TTTTGGACCC TCATTTGGCC
    AAGCACTTAG CACATTTTGG AATTGACATG CTTCATATGC ATGGGACAGA GAATGGGCTC
    CAGGACAACG ACATCAAGCC GCGGGTCAGC GAGTGGGAGG TGATCCAGGA GACGGGGACA
    AAGCTGAAGC CCATGTACGG CCCAGGGTAC ACAGGGCTCA AGAACCTGGG CAATAGCTGC
    TATCTCAGCT CTGTGATGCA GGCCATCTTC AGCATCCCAG AATTCCAGAG AGCGTATGTA
    GGAAATCTTC CAAGAATATT TGACTACTCT CCTTTAGATC CAACACAAGA TTTCAACACA
    CAGATGACTA AGTTAGGACA TGGCCTTCTC TCAGGCCAGT ATTCAAAACC TCCCGTGAAG
    TCTGAGCTCA TTGAACAGGT GATGAAGGAG GAACACAAGC CTCAACAGAA TGGGATCTCT
    CCACGCATGT TTAAGGCCTT TGTGAGCAAG AGCCACCCGG AATTCTCCTC CAACAGACAG
    CAGGATGCCC AGGAGTTTTT CCTGCACCTG GTGAATCTAG TAGAGAGGAA CCGCATTGGC
    TCAGAAAACC CAAGTGATGT TTTTCGGTTT TTGGTGGAAG AGCGGATTCA GTGCTGTCAG
    ACCCGAAAAG TCCGCTACAC GGAGAGGGTG GACTACCTGA TGCAGTTACC TGTGGCCATG
    GAGGCAGCAA CCAACAAAGA TGAACTGATC GCCTATGAAC TAACGAGAAG GGAAGCAGAA
    TCAAACAGAA GACCCCTTCC CGAGTTGGTT CGTGCCAAGA TACCATTCAG TGCCTGCCTG
    CAGGCCTTTT CTGAACCAGA AAATGTTGAT GATTTCTGGA GCAGCGCCCT GCAAGCAAAG
    TCTGCGGGTG TGAAAACATC TCGCTTTGCC TCATTCCCTG AATACTTGGT AGTGCAGATA
    AAGAAGTTCA CTTTTGGTCT TGACTGGGTT CCCAAAAAAT TTGATGTTTC TGTTGATATG
    CCAGACCTAC TTGATATCAA CCATCTCCGA GCCAGGGGGT TGCAGCCAGG AGAGGAGGAA
    CTTCCAGACA TCAGCCCCCC CATAGTGATT CCTGATGACT CTAAAGATCG CCTGATGACC
    CAATTGATAG ACCCATCAGA TATTGATGAG TCTTCGGTGA TGCAGCTGGC TGAGATGGGC
    TTCCCTCTAG AAGCATGTCG GAAGGCTGTG TATTTTACTG GAAATATGGG AGCCGAGGTG
    GCCTTCAACT GGATCGTTGT TCACATGGAA GAGCCAGATT TTGCTGAACC GCTGACCATG
    CCTGGTTATG GAGGAGCAGC TTCTGCTGGG GCCTCTGTTT TTGGTGCTAC TGGATTGGAT
    AACCAACCTC CGGAAGAAAC TGTAGCTATC ATTACCTCCA TGGGATTCCA TCGAAATCAG
    GCTATTCAGG CCCTGCGAGC AACGAATAGT AACCTGGAAA GAGCACTGGA TTGGATCTTT
    AGCCACCCTG AGTTTGAGGA GGACAGTGAC TTTGTGATCG AGATGGAGAA TAACGCCAAT
    GCGAACATCG TTTCTGAGGC CAAACCTGAA GGACCTAGAG TCAAGGATGG ATCTGGAATG
    TATGAATTAT TTGCGTTCAT CAGTCACATG GGAACGTCTA CAATGAGTGG CCACTACGTT
    TGTCACATTA AAAAGGAAGG AAGATGGGTG ATCTACAATG ACCACAAAGT TTGTGCCTCA
    GAAAGGCCCC CTAAAGACCT GGGCTACATG TACTTTTACC GCAGGATACC AAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216421
    Clone ID Related Accession (Same CDS sequence) NM_001191266.2
    Accession Version NM_001191266.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-23
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 13
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000001.1. On Jun 30, 2017 this sequence version replaced NM_001191266.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721697.62.1, SRR5571255.17026.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGCGCC GGGGCGCCCT GTTCGGCATG CCGGGCGGTA GCGGCAGCAG GAAGATGGCT 
    GCAGGAGACA TCGGCGAGCT GCTAGTGCCC CACATGCCCA CGATCCGCGT GCCCAGGTCC
    GGGGACAGGG TCTACAAGAA CGAGTGCGCC TTCTCCTACG ACTCCCCGAA TTCTGAAGGC
    GGACTCTATG TCTGCATGAA TACATTTTTG GCCTTTGGAA GGGAACACGT GGAAAGACAC
    TTTCGAAAAA CTGGACAGAG TGTCTACATG CATCTGAAGA GACACGTGCG CGAGAAGGTA
    AGAGGCGCAT CTGGTGGCGC TTTACCCAAA AGGAGGAATT CCAAGATGTT TTTAGATCTA
    GATACTGATG ACGATTTAAA TAGCGACGAT TATGAATATG AAGATGAAGC CAAACTTGTT
    ATATTCCCAG ACCACTATGA AATAGCCCTA CCAAATATTG AGGAGTTACC AGCCCTGGTA
    ACCATTGCTT GTGATGCAGT TCTCAGCTCA AAATCTCCAT ACAGAAAGCA GGACCCAGAT
    ACATGGGAGA ATGAATTGCC AGTGTCGAAA TATGCCAATA ACCTTACCCA GTTGGACAAT
    GGAGTCAGAA TTCCTCCAAG TGGTTGGAAG TGTGCCAGAT GCGACCTTCG GGAGAACCTC
    TGGTTGAACC TGACCGATGG CTCCGTGCTG TGTGGGAAGT GGTTCTTTGA CAGCTCTGGG
    GGCAACGGGC ATGCGTTGGA GCATTACAGA GACACAGGCT ACCCTCTCGC TGTGAAACTG
    GGGACCATCA CTCCGGATGG GGCCGATGTT TATTCTTTCC AAGAAGAGGA AGCTGTTTTG
    GACCCTCATT TGGCCAAGCA CTTAGCACAT TTTGGAATTG ACATGCTTCA TATGCATGGG
    ACAGAGAATG GGCTCCAGGA CAACGACATC AAGCCGCGGG TCAGCGAGTG GGAGGTGATC
    CAGGAGACGG GGACAAAGCT GAAGCCCATG TACGGCCCAG GGTACACAGG GCTCAAGAAC
    CTGGGCAATA GCTGCTATCT CAGCTCTGTG ATGCAGGCCA TCTTCAGCAT CCCAGAATTC
    CAGAGAGCGT ATGTAGGAAA TCTTCCAAGA ATATTTGACT ACTCTCCTTT AGATCCAACA
    CAAGATTTCA ACACACAGAT GACTAAGTTA GGACATGGCC TTCTCTCAGG CCAGTATTCA
    AAACCTCCCG TGAAGTCTGA GCTCATTGAA CAGGTGATGA AGGAGGAACA CAAGCCTCAA
    CAGAATGGGA TCTCTCCACG CATGTTTAAG GCCTTTGTGA GCAAGAGCCA CCCGGAATTC
    TCCTCCAACA GACAGCAGGA TGCCCAGGAG TTTTTCCTGC ACCTGGTGAA TCTAGTAGAG
    AGGAACCGCA TTGGCTCAGA AAACCCAAGT GATGTTTTTC GGTTTTTGGT GGAAGAGCGG
    ATTCAGTGCT GTCAGACCCG AAAAGTCCGC TACACGGAGA GGGTGGACTA CCTGATGCAG
    TTACCTGTGG CCATGGAGGC AGCAACCAAC AAAGATGAAC TGATCGCCTA TGAACTAACG
    AGAAGGGAAG CAGAATCAAA CAGAAGACCC CTTCCCGAGT TGGTTCGTGC CAAGATACCA
    TTCAGTGCCT GCCTGCAGGC CTTTTCTGAA CCAGAAAATG TTGATGATTT CTGGAGCAGC
    GCCCTGCAAG CAAAGTCTGC GGGTGTGAAA ACATCTCGCT TTGCCTCATT CCCTGAATAC
    TTGGTAGTGC AGATAAAGAA GTTCACTTTT GGTCTTGACT GGGTTCCCAA AAAATTTGAT
    GTTTCTGTTG ATATGCCAGA CCTACTTGAT ATCAACCATC TCCGAGCCAG GGGGTTGCAG
    CCAGGAGAGG AGGAACTTCC AGACATCAGC CCCCCCATAG TGATTCCTGA TGACTCTAAA
    GATCGCCTGA TGACCCAATT GATAGACCCA TCAGATATTG ATGAGTCTTC GGTGATGCAG
    CTGGCTGAGA TGGGCTTCCC TCTAGAAGCA TGTCGGAAGG CTGTGTATTT TACTGGAAAT
    ATGGGAGCCG AGGTGGCCTT CAACTGGATC GTTGTTCACA TGGAAGAGCC AGATTTTGCT
    GAACCGCTGA CCATGCCTGG TTATGGAGGA GCAGCTTCTG CTGGGGCCTC TGTTTTTGGT
    GCTACTGGAT TGGATAACCA ACCTCCGGAA GAAACTGTAG CTATCATTAC CTCCATGGGA
    TTCCATCGAA ATCAGGCTAT TCAGGCCCTG CGAGCAACGA ATAGTAACCT GGAAAGAGCA
    CTGGATTGGA TCTTTAGCCA CCCTGAGTTT GAGGAGGACA GTGACTTTGT GATCGAGATG
    GAGAATAACG CCAATGCGAA CATCGTTTCT GAGGCCAAAC CTGAAGGACC TAGAGTCAAG
    GATGGATCTG GAATGTATGA ATTATTTGCG TTCATCAGTC ACATGGGAAC GTCTACAATG
    AGTGGCCACT ACGTTTGTCA CATTAAAAAG GAAGGAAGAT GGGTGATCTA CAATGACCAC
    AAAGTTTGTG CCTCAGAAAG GCCCCCTAAA GACCTGGGCT ACATGTACTT TTACCGCAGG
    ATACCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178195.1
    CDS80..2671
    Translation

    Target ORF information:

    RefSeq Version NM_001191266.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 13 (USP13), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191266.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCAGCGCC GGGGCGCCCT GTTCGGCATG CCGGGCGGTA GCGGCAGCAG GAAGATGGCT 
    GCAGGAGACA TCGGCGAGCT GCTAGTGCCC CACATGCCCA CGATCCGCGT GCCCAGGTCC
    GGGGACAGGG TCTACAAGAA CGAGTGCGCC TTCTCCTACG ACTCCCCGAA TTCTGAAGGC
    GGACTCTATG TCTGCATGAA TACATTTTTG GCCTTTGGAA GGGAACACGT GGAAAGACAC
    TTTCGAAAAA CTGGACAGAG TGTCTACATG CATCTGAAGA GACACGTGCG CGAGAAGGTA
    AGAGGCGCAT CTGGTGGCGC TTTACCCAAA AGGAGGAATT CCAAGATGTT TTTAGATCTA
    GATACTGATG ACGATTTAAA TAGCGACGAT TATGAATATG AAGATGAAGC CAAACTTGTT
    ATATTCCCAG ACCACTATGA AATAGCCCTA CCAAATATTG AGGAGTTACC AGCCCTGGTA
    ACCATTGCTT GTGATGCAGT TCTCAGCTCA AAATCTCCAT ACAGAAAGCA GGACCCAGAT
    ACATGGGAGA ATGAATTGCC AGTGTCGAAA TATGCCAATA ACCTTACCCA GTTGGACAAT
    GGAGTCAGAA TTCCTCCAAG TGGTTGGAAG TGTGCCAGAT GCGACCTTCG GGAGAACCTC
    TGGTTGAACC TGACCGATGG CTCCGTGCTG TGTGGGAAGT GGTTCTTTGA CAGCTCTGGG
    GGCAACGGGC ATGCGTTGGA GCATTACAGA GACACAGGCT ACCCTCTCGC TGTGAAACTG
    GGGACCATCA CTCCGGATGG GGCCGATGTT TATTCTTTCC AAGAAGAGGA AGCTGTTTTG
    GACCCTCATT TGGCCAAGCA CTTAGCACAT TTTGGAATTG ACATGCTTCA TATGCATGGG
    ACAGAGAATG GGCTCCAGGA CAACGACATC AAGCCGCGGG TCAGCGAGTG GGAGGTGATC
    CAGGAGACGG GGACAAAGCT GAAGCCCATG TACGGCCCAG GGTACACAGG GCTCAAGAAC
    CTGGGCAATA GCTGCTATCT CAGCTCTGTG ATGCAGGCCA TCTTCAGCAT CCCAGAATTC
    CAGAGAGCGT ATGTAGGAAA TCTTCCAAGA ATATTTGACT ACTCTCCTTT AGATCCAACA
    CAAGATTTCA ACACACAGAT GACTAAGTTA GGACATGGCC TTCTCTCAGG CCAGTATTCA
    AAACCTCCCG TGAAGTCTGA GCTCATTGAA CAGGTGATGA AGGAGGAACA CAAGCCTCAA
    CAGAATGGGA TCTCTCCACG CATGTTTAAG GCCTTTGTGA GCAAGAGCCA CCCGGAATTC
    TCCTCCAACA GACAGCAGGA TGCCCAGGAG TTTTTCCTGC ACCTGGTGAA TCTAGTAGAG
    AGGAACCGCA TTGGCTCAGA AAACCCAAGT GATGTTTTTC GGTTTTTGGT GGAAGAGCGG
    ATTCAGTGCT GTCAGACCCG AAAAGTCCGC TACACGGAGA GGGTGGACTA CCTGATGCAG
    TTACCTGTGG CCATGGAGGC AGCAACCAAC AAAGATGAAC TGATCGCCTA TGAACTAACG
    AGAAGGGAAG CAGAATCAAA CAGAAGACCC CTTCCCGAGT TGGTTCGTGC CAAGATACCA
    TTCAGTGCCT GCCTGCAGGC CTTTTCTGAA CCAGAAAATG TTGATGATTT CTGGAGCAGC
    GCCCTGCAAG CAAAGTCTGC GGGTGTGAAA ACATCTCGCT TTGCCTCATT CCCTGAATAC
    TTGGTAGTGC AGATAAAGAA GTTCACTTTT GGTCTTGACT GGGTTCCCAA AAAATTTGAT
    GTTTCTGTTG ATATGCCAGA CCTACTTGAT ATCAACCATC TCCGAGCCAG GGGGTTGCAG
    CCAGGAGAGG AGGAACTTCC AGACATCAGC CCCCCCATAG TGATTCCTGA TGACTCTAAA
    GATCGCCTGA TGACCCAATT GATAGACCCA TCAGATATTG ATGAGTCTTC GGTGATGCAG
    CTGGCTGAGA TGGGCTTCCC TCTAGAAGCA TGTCGGAAGG CTGTGTATTT TACTGGAAAT
    ATGGGAGCCG AGGTGGCCTT CAACTGGATC GTTGTTCACA TGGAAGAGCC AGATTTTGCT
    GAACCGCTGA CCATGCCTGG TTATGGAGGA GCAGCTTCTG CTGGGGCCTC TGTTTTTGGT
    GCTACTGGAT TGGATAACCA ACCTCCGGAA GAAACTGTAG CTATCATTAC CTCCATGGGA
    TTCCATCGAA ATCAGGCTAT TCAGGCCCTG CGAGCAACGA ATAGTAACCT GGAAAGAGCA
    CTGGATTGGA TCTTTAGCCA CCCTGAGTTT GAGGAGGACA GTGACTTTGT GATCGAGATG
    GAGAATAACG CCAATGCGAA CATCGTTTCT GAGGCCAAAC CTGAAGGACC TAGAGTCAAG
    GATGGATCTG GAATGTATGA ATTATTTGCG TTCATCAGTC ACATGGGAAC GTCTACAATG
    AGTGGCCACT ACGTTTGTCA CATTAAAAAG GAAGGAAGAT GGGTGATCTA CAATGACCAC
    AAAGTTTGTG CCTCAGAAAG GCCCCCTAAA GACCTGGGCT ACATGTACTT TTACCGCAGG
    ATACCAAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87765
    Clone ID Related Accession (Same CDS sequence) XM_015472368.1 , XM_015472368.2
    Accession Version XM_015472368.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 13 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103814.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGATA ATTCTGAAGG CGGACTCTAT GTCTGCATGA ATACATTTTT GGCCTTTGGA 
    AGGGAACACG TGGAAAGACA CTTTCGAAAA ACTGGACAGA GTGTCTACAT GCATCTGAAG
    AGACACGTGC GCGAGAAGGT AAGAGGCGCA TCTGGTGGCG CTTTACCCAA AAGGAGGAAT
    TCCAAGATGT TTTTAGATCT AGATACTGAT GACGATTTAA ATAGCGACGA TTATGAATAT
    GAAGATGAAG CCAAACTTGT TATATTCCCA GACCACTATG AAATAGCCCT ACCAAATATT
    GAGGAGTTAC CAGCCCTGGT AACCATTGCT TGTGATGCAG TTCTCAGCTC AAAATCTCCA
    TACAGAAAGC AGGACCCAGA TACATGGGAG AATGAATTGC CAGTGTCGAA ATATGCCAAT
    AACCTTACCC AGTTGGACAA TGGAGTCAGA ATTCCTCCAA GTGGTTGGAA GTGTGCCAGA
    TGCGACCTTC GGGAGAACCT CTGGTTGAAC CTGACCGATG GCTCCGTGCT GTGTGGGAAG
    TGGTTCTTTG ACAGCTCTGG GGGCAACGGG CATGCGTTGG AGCATTACAG AGACACAGGC
    TACCCTCTCG CTGTGAAACT GGGGACCATC ACTCCGGATG GGGCCGATGT TTATTCTTTC
    CAAGAAGAGG AAGCTGTTTT GGACCCTCAT TTGGCCAAGC ACTTAGCACA TTTTGGAATT
    GACATGCTTC ATATGCATGG GACAGAGAAT GGGCTCCAGG ACAACGACAT CAAGCCGCGG
    GTCAGCGAGT GGGAGGTGAT CCAGGAGACG GGGACAAAGC TGAAGCCCAT GTACGGCCCA
    GGGTACACAG GGCTCAAGAA CCTGGGCAAT AGCTGCTATC TCAGCTCTGT GATGCAGGCC
    ATCTTCAGCA TCCCAGAATT CCAGAGAGCG TATGTAGGAA ATCTTCCAAG AATATTTGAC
    TACTCTCCTT TAGATCCAAC ACAAGATTTC AACACACAGA TGACTAAGTT AGGACATGGC
    CTTCTCTCAG GCCAGTATTC AAAACCTCCC GTGAAGTCTG AGCTCATTGA ACAGGTGATG
    AAGGAGGAAC ACAAGCCTCA ACAGAATGGG ATCTCTCCAC GCATGTTTAA GGCCTTTGTG
    AGCAAGAGCC ACCCGGAATT CTCCTCCAAC AGACAGCAGG ATGCCCAGGA GTTTTTCCTG
    CACCTGGTGA ATCTAGTAGA GAGGAACCGC ATTGGCTCAG AAAACCCAAG TGATGTTTTT
    CGGTTTTTGG TGGAAGAGCG GATTCAGTGC TGTCAGACCC GAAAAGTCCG CTACACGGAG
    AGGGTGGACT ACCTGATGCA GTTACCTGTG GCCATGGAGG CAGCAACCAA CAAAGATGAA
    CTGATCGCCT ATGAACTAAC GAGAAGGGAA GCAGAATCAA ACAGAAGACC CCTTCCCGAG
    TTGGTTCGTG CCAAGATACC ATTCAGTGCC TGCCTGCAGG CCTTTTCTGA ACCAGAAAAT
    GTTGATGATT TCTGGAGCAG CGCCCTGCAA GCAAAGTCTG CGGGTGTGAA AACATCTCGC
    TTTGCCTCAT TCCCTGAATA CTTGGTAGTG CAGATAAAGA AGTTCACTTT TGGTCTTGAC
    TGGGTTCCCA AAAAATTTGA TGTTTCTGTT GATATGCCAG ACCTACTTGA TATCAACCAT
    CTCCGAGCCA GGGGGTTGCA GCCAGGAGAG GAGGAACTTC CAGACATCAG CCCCCCCATA
    GTGATTCCTG ATGACTCTAA AGATCGCCTG ATGACCCAAT TGATAGACCC ATCAGATATT
    GATGAGTCTT CGGTGATGCA GCTGGCTGAG ATGGGCTTCC CTCTAGAAGC ATGTCGGAAG
    GCTGTGTATT TTACTGGAAA TATGGGAGCC GAGGTGGCCT TCAACTGGAT CGTTGTTCAC
    ATGGAAGAGC CAGATTTTGC TGAACCGCTG ACCATGCCTG GTTATGGAGG AGCAGCTTCT
    GCTGGGGCCT CTGTTTTTGG TGCTACTGGA TTGGATAACC AACCTCCGGA AGAAACTGTA
    GCTATCATTA CCTCCATGGG ATTCCATCGA AATCAGGCTA TTCAGGCCCT GCGAGCAACG
    AATAGTAACC TGGAAAGAGC ACTGGATTGG ATCTTTAGCC ACCCTGAGTT TGAGGAGGAC
    AGTGACTTTG TGATCGAGAT GGAGAATAAC GCCAATGCGA ACATCGTTTC TGAGGCCAAA
    CCTGAAGGAC CTAGAGTCAA GGATGGATCT GGAATGTATG AATTATTTGC GTTCATCAGT
    CACATGGGAA CGTCTACAAT GAGTGGCCAC TACGTTTGTC ACATTAAAAA GGAAGGAAGA
    TGGGTGATCT ACAATGACCA CAAAGTTTGT GCCTCAGAAA GGCCCCCTAA AGACCTGGGC
    TACATGTACT TTTACCGCAG GATACCAAGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327854.1
    CDS252..2684
    Translation

    Target ORF information:

    RefSeq Version XM_015472368.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGATA ATTCTGAAGG CGGACTCTAT GTCTGCATGA ATACATTTTT GGCCTTTGGA 
    AGGGAACACG TGGAAAGACA CTTTCGAAAA ACTGGACAGA GTGTCTACAT GCATCTGAAG
    AGACACGTGC GCGAGAAGGT AAGAGGCGCA TCTGGTGGCG CTTTACCCAA AAGGAGGAAT
    TCCAAGATGT TTTTAGATCT AGATACTGAT GACGATTTAA ATAGCGACGA TTATGAATAT
    GAAGATGAAG CCAAACTTGT TATATTCCCA GACCACTATG AAATAGCCCT ACCAAATATT
    GAGGAGTTAC CAGCCCTGGT AACCATTGCT TGTGATGCAG TTCTCAGCTC AAAATCTCCA
    TACAGAAAGC AGGACCCAGA TACATGGGAG AATGAATTGC CAGTGTCGAA ATATGCCAAT
    AACCTTACCC AGTTGGACAA TGGAGTCAGA ATTCCTCCAA GTGGTTGGAA GTGTGCCAGA
    TGCGACCTTC GGGAGAACCT CTGGTTGAAC CTGACCGATG GCTCCGTGCT GTGTGGGAAG
    TGGTTCTTTG ACAGCTCTGG GGGCAACGGG CATGCGTTGG AGCATTACAG AGACACAGGC
    TACCCTCTCG CTGTGAAACT GGGGACCATC ACTCCGGATG GGGCCGATGT TTATTCTTTC
    CAAGAAGAGG AAGCTGTTTT GGACCCTCAT TTGGCCAAGC ACTTAGCACA TTTTGGAATT
    GACATGCTTC ATATGCATGG GACAGAGAAT GGGCTCCAGG ACAACGACAT CAAGCCGCGG
    GTCAGCGAGT GGGAGGTGAT CCAGGAGACG GGGACAAAGC TGAAGCCCAT GTACGGCCCA
    GGGTACACAG GGCTCAAGAA CCTGGGCAAT AGCTGCTATC TCAGCTCTGT GATGCAGGCC
    ATCTTCAGCA TCCCAGAATT CCAGAGAGCG TATGTAGGAA ATCTTCCAAG AATATTTGAC
    TACTCTCCTT TAGATCCAAC ACAAGATTTC AACACACAGA TGACTAAGTT AGGACATGGC
    CTTCTCTCAG GCCAGTATTC AAAACCTCCC GTGAAGTCTG AGCTCATTGA ACAGGTGATG
    AAGGAGGAAC ACAAGCCTCA ACAGAATGGG ATCTCTCCAC GCATGTTTAA GGCCTTTGTG
    AGCAAGAGCC ACCCGGAATT CTCCTCCAAC AGACAGCAGG ATGCCCAGGA GTTTTTCCTG
    CACCTGGTGA ATCTAGTAGA GAGGAACCGC ATTGGCTCAG AAAACCCAAG TGATGTTTTT
    CGGTTTTTGG TGGAAGAGCG GATTCAGTGC TGTCAGACCC GAAAAGTCCG CTACACGGAG
    AGGGTGGACT ACCTGATGCA GTTACCTGTG GCCATGGAGG CAGCAACCAA CAAAGATGAA
    CTGATCGCCT ATGAACTAAC GAGAAGGGAA GCAGAATCAA ACAGAAGACC CCTTCCCGAG
    TTGGTTCGTG CCAAGATACC ATTCAGTGCC TGCCTGCAGG CCTTTTCTGA ACCAGAAAAT
    GTTGATGATT TCTGGAGCAG CGCCCTGCAA GCAAAGTCTG CGGGTGTGAA AACATCTCGC
    TTTGCCTCAT TCCCTGAATA CTTGGTAGTG CAGATAAAGA AGTTCACTTT TGGTCTTGAC
    TGGGTTCCCA AAAAATTTGA TGTTTCTGTT GATATGCCAG ACCTACTTGA TATCAACCAT
    CTCCGAGCCA GGGGGTTGCA GCCAGGAGAG GAGGAACTTC CAGACATCAG CCCCCCCATA
    GTGATTCCTG ATGACTCTAA AGATCGCCTG ATGACCCAAT TGATAGACCC ATCAGATATT
    GATGAGTCTT CGGTGATGCA GCTGGCTGAG ATGGGCTTCC CTCTAGAAGC ATGTCGGAAG
    GCTGTGTATT TTACTGGAAA TATGGGAGCC GAGGTGGCCT TCAACTGGAT CGTTGTTCAC
    ATGGAAGAGC CAGATTTTGC TGAACCGCTG ACCATGCCTG GTTATGGAGG AGCAGCTTCT
    GCTGGGGCCT CTGTTTTTGG TGCTACTGGA TTGGATAACC AACCTCCGGA AGAAACTGTA
    GCTATCATTA CCTCCATGGG ATTCCATCGA AATCAGGCTA TTCAGGCCCT GCGAGCAACG
    AATAGTAACC TGGAAAGAGC ACTGGATTGG ATCTTTAGCC ACCCTGAGTT TGAGGAGGAC
    AGTGACTTTG TGATCGAGAT GGAGAATAAC GCCAATGCGA ACATCGTTTC TGAGGCCAAA
    CCTGAAGGAC CTAGAGTCAA GGATGGATCT GGAATGTATG AATTATTTGC GTTCATCAGT
    CACATGGGAA CGTCTACAAT GAGTGGCCAC TACGTTTGTC ACATTAAAAA GGAAGGAAGA
    TGGGTGATCT ACAATGACCA CAAAGTTTGT GCCTCAGAAA GGCCCCCTAA AGACCTGGGC
    TACATGTACT TTTACCGCAG GATACCAAGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87765
    Clone ID Related Accession (Same CDS sequence) XM_015472368.1 , XM_015472368.2
    Accession Version XM_015472368.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ubiquitin carboxyl-terminal hydrolase 13 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGATA ATTCTGAAGG CGGACTCTAT GTCTGCATGA ATACATTTTT GGCCTTTGGA 
    AGGGAACACG TGGAAAGACA CTTTCGAAAA ACTGGACAGA GTGTCTACAT GCATCTGAAG
    AGACACGTGC GCGAGAAGGT AAGAGGCGCA TCTGGTGGCG CTTTACCCAA AAGGAGGAAT
    TCCAAGATGT TTTTAGATCT AGATACTGAT GACGATTTAA ATAGCGACGA TTATGAATAT
    GAAGATGAAG CCAAACTTGT TATATTCCCA GACCACTATG AAATAGCCCT ACCAAATATT
    GAGGAGTTAC CAGCCCTGGT AACCATTGCT TGTGATGCAG TTCTCAGCTC AAAATCTCCA
    TACAGAAAGC AGGACCCAGA TACATGGGAG AATGAATTGC CAGTGTCGAA ATATGCCAAT
    AACCTTACCC AGTTGGACAA TGGAGTCAGA ATTCCTCCAA GTGGTTGGAA GTGTGCCAGA
    TGCGACCTTC GGGAGAACCT CTGGTTGAAC CTGACCGATG GCTCCGTGCT GTGTGGGAAG
    TGGTTCTTTG ACAGCTCTGG GGGCAACGGG CATGCGTTGG AGCATTACAG AGACACAGGC
    TACCCTCTCG CTGTGAAACT GGGGACCATC ACTCCGGATG GGGCCGATGT TTATTCTTTC
    CAAGAAGAGG AAGCTGTTTT GGACCCTCAT TTGGCCAAGC ACTTAGCACA TTTTGGAATT
    GACATGCTTC ATATGCATGG GACAGAGAAT GGGCTCCAGG ACAACGACAT CAAGCCGCGG
    GTCAGCGAGT GGGAGGTGAT CCAGGAGACG GGGACAAAGC TGAAGCCCAT GTACGGCCCA
    GGGTACACAG GGCTCAAGAA CCTGGGCAAT AGCTGCTATC TCAGCTCTGT GATGCAGGCC
    ATCTTCAGCA TCCCAGAATT CCAGAGAGCG TATGTAGGAA ATCTTCCAAG AATATTTGAC
    TACTCTCCTT TAGATCCAAC ACAAGATTTC AACACACAGA TGACTAAGTT AGGACATGGC
    CTTCTCTCAG GCCAGTATTC AAAACCTCCC GTGAAGTCTG AGCTCATTGA ACAGGTGATG
    AAGGAGGAAC ACAAGCCTCA ACAGAATGGG ATCTCTCCAC GCATGTTTAA GGCCTTTGTG
    AGCAAGAGCC ACCCGGAATT CTCCTCCAAC AGACAGCAGG ATGCCCAGGA GTTTTTCCTG
    CACCTGGTGA ATCTAGTAGA GAGGAACCGC ATTGGCTCAG AAAACCCAAG TGATGTTTTT
    CGGTTTTTGG TGGAAGAGCG GATTCAGTGC TGTCAGACCC GAAAAGTCCG CTACACGGAG
    AGGGTGGACT ACCTGATGCA GTTACCTGTG GCCATGGAGG CAGCAACCAA CAAAGATGAA
    CTGATCGCCT ATGAACTAAC GAGAAGGGAA GCAGAATCAA ACAGAAGACC CCTTCCCGAG
    TTGGTTCGTG CCAAGATACC ATTCAGTGCC TGCCTGCAGG CCTTTTCTGA ACCAGAAAAT
    GTTGATGATT TCTGGAGCAG CGCCCTGCAA GCAAAGTCTG CGGGTGTGAA AACATCTCGC
    TTTGCCTCAT TCCCTGAATA CTTGGTAGTG CAGATAAAGA AGTTCACTTT TGGTCTTGAC
    TGGGTTCCCA AAAAATTTGA TGTTTCTGTT GATATGCCAG ACCTACTTGA TATCAACCAT
    CTCCGAGCCA GGGGGTTGCA GCCAGGAGAG GAGGAACTTC CAGACATCAG CCCCCCCATA
    GTGATTCCTG ATGACTCTAA AGATCGCCTG ATGACCCAAT TGATAGACCC ATCAGATATT
    GATGAGTCTT CGGTGATGCA GCTGGCTGAG ATGGGCTTCC CTCTAGAAGC ATGTCGGAAG
    GCTGTGTATT TTACTGGAAA TATGGGAGCC GAGGTGGCCT TCAACTGGAT CGTTGTTCAC
    ATGGAAGAGC CAGATTTTGC TGAACCGCTG ACCATGCCTG GTTATGGAGG AGCAGCTTCT
    GCTGGGGCCT CTGTTTTTGG TGCTACTGGA TTGGATAACC AACCTCCGGA AGAAACTGTA
    GCTATCATTA CCTCCATGGG ATTCCATCGA AATCAGGCTA TTCAGGCCCT GCGAGCAACG
    AATAGTAACC TGGAAAGAGC ACTGGATTGG ATCTTTAGCC ACCCTGAGTT TGAGGAGGAC
    AGTGACTTTG TGATCGAGAT GGAGAATAAC GCCAATGCGA ACATCGTTTC TGAGGCCAAA
    CCTGAAGGAC CTAGAGTCAA GGATGGATCT GGAATGTATG AATTATTTGC GTTCATCAGT
    CACATGGGAA CGTCTACAAT GAGTGGCCAC TACGTTTGTC ACATTAAAAA GGAAGGAAGA
    TGGGTGATCT ACAATGACCA CAAAGTTTGT GCCTCAGAAA GGCCCCCTAA AGACCTGGGC
    TACATGTACT TTTACCGCAG GATACCAAGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327854.1
    CDS428..2860
    Translation

    Target ORF information:

    RefSeq Version XM_015472368.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin specific peptidase 13 (USP13), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472368.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGATA ATTCTGAAGG CGGACTCTAT GTCTGCATGA ATACATTTTT GGCCTTTGGA 
    AGGGAACACG TGGAAAGACA CTTTCGAAAA ACTGGACAGA GTGTCTACAT GCATCTGAAG
    AGACACGTGC GCGAGAAGGT AAGAGGCGCA TCTGGTGGCG CTTTACCCAA AAGGAGGAAT
    TCCAAGATGT TTTTAGATCT AGATACTGAT GACGATTTAA ATAGCGACGA TTATGAATAT
    GAAGATGAAG CCAAACTTGT TATATTCCCA GACCACTATG AAATAGCCCT ACCAAATATT
    GAGGAGTTAC CAGCCCTGGT AACCATTGCT TGTGATGCAG TTCTCAGCTC AAAATCTCCA
    TACAGAAAGC AGGACCCAGA TACATGGGAG AATGAATTGC CAGTGTCGAA ATATGCCAAT
    AACCTTACCC AGTTGGACAA TGGAGTCAGA ATTCCTCCAA GTGGTTGGAA GTGTGCCAGA
    TGCGACCTTC GGGAGAACCT CTGGTTGAAC CTGACCGATG GCTCCGTGCT GTGTGGGAAG
    TGGTTCTTTG ACAGCTCTGG GGGCAACGGG CATGCGTTGG AGCATTACAG AGACACAGGC
    TACCCTCTCG CTGTGAAACT GGGGACCATC ACTCCGGATG GGGCCGATGT TTATTCTTTC
    CAAGAAGAGG AAGCTGTTTT GGACCCTCAT TTGGCCAAGC ACTTAGCACA TTTTGGAATT
    GACATGCTTC ATATGCATGG GACAGAGAAT GGGCTCCAGG ACAACGACAT CAAGCCGCGG
    GTCAGCGAGT GGGAGGTGAT CCAGGAGACG GGGACAAAGC TGAAGCCCAT GTACGGCCCA
    GGGTACACAG GGCTCAAGAA CCTGGGCAAT AGCTGCTATC TCAGCTCTGT GATGCAGGCC
    ATCTTCAGCA TCCCAGAATT CCAGAGAGCG TATGTAGGAA ATCTTCCAAG AATATTTGAC
    TACTCTCCTT TAGATCCAAC ACAAGATTTC AACACACAGA TGACTAAGTT AGGACATGGC
    CTTCTCTCAG GCCAGTATTC AAAACCTCCC GTGAAGTCTG AGCTCATTGA ACAGGTGATG
    AAGGAGGAAC ACAAGCCTCA ACAGAATGGG ATCTCTCCAC GCATGTTTAA GGCCTTTGTG
    AGCAAGAGCC ACCCGGAATT CTCCTCCAAC AGACAGCAGG ATGCCCAGGA GTTTTTCCTG
    CACCTGGTGA ATCTAGTAGA GAGGAACCGC ATTGGCTCAG AAAACCCAAG TGATGTTTTT
    CGGTTTTTGG TGGAAGAGCG GATTCAGTGC TGTCAGACCC GAAAAGTCCG CTACACGGAG
    AGGGTGGACT ACCTGATGCA GTTACCTGTG GCCATGGAGG CAGCAACCAA CAAAGATGAA
    CTGATCGCCT ATGAACTAAC GAGAAGGGAA GCAGAATCAA ACAGAAGACC CCTTCCCGAG
    TTGGTTCGTG CCAAGATACC ATTCAGTGCC TGCCTGCAGG CCTTTTCTGA ACCAGAAAAT
    GTTGATGATT TCTGGAGCAG CGCCCTGCAA GCAAAGTCTG CGGGTGTGAA AACATCTCGC
    TTTGCCTCAT TCCCTGAATA CTTGGTAGTG CAGATAAAGA AGTTCACTTT TGGTCTTGAC
    TGGGTTCCCA AAAAATTTGA TGTTTCTGTT GATATGCCAG ACCTACTTGA TATCAACCAT
    CTCCGAGCCA GGGGGTTGCA GCCAGGAGAG GAGGAACTTC CAGACATCAG CCCCCCCATA
    GTGATTCCTG ATGACTCTAA AGATCGCCTG ATGACCCAAT TGATAGACCC ATCAGATATT
    GATGAGTCTT CGGTGATGCA GCTGGCTGAG ATGGGCTTCC CTCTAGAAGC ATGTCGGAAG
    GCTGTGTATT TTACTGGAAA TATGGGAGCC GAGGTGGCCT TCAACTGGAT CGTTGTTCAC
    ATGGAAGAGC CAGATTTTGC TGAACCGCTG ACCATGCCTG GTTATGGAGG AGCAGCTTCT
    GCTGGGGCCT CTGTTTTTGG TGCTACTGGA TTGGATAACC AACCTCCGGA AGAAACTGTA
    GCTATCATTA CCTCCATGGG ATTCCATCGA AATCAGGCTA TTCAGGCCCT GCGAGCAACG
    AATAGTAACC TGGAAAGAGC ACTGGATTGG ATCTTTAGCC ACCCTGAGTT TGAGGAGGAC
    AGTGACTTTG TGATCGAGAT GGAGAATAAC GCCAATGCGA ACATCGTTTC TGAGGCCAAA
    CCTGAAGGAC CTAGAGTCAA GGATGGATCT GGAATGTATG AATTATTTGC GTTCATCAGT
    CACATGGGAA CGTCTACAAT GAGTGGCCAC TACGTTTGTC ACATTAAAAA GGAAGGAAGA
    TGGGTGATCT ACAATGACCA CAAAGTTTGT GCCTCAGAAA GGCCCCCTAA AGACCTGGGC
    TACATGTACT TTTACCGCAG GATACCAAGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of taurine cattle: a window to ruminant biology and evolution.
    Science (New York, N.Y.)324(5926)522-8(2009 Apr)
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    A whole-genome assembly of the domestic cow, Bos taurus.
    Genome biology10(4)R42(2009)
    Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL