ASXL2 cDNA ORF clone, Bos taurus(cattle)

The following ASXL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASXL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa06978 XM_005213081.4
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06978 XM_005193939.3
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06979 XM_010817016.2
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06979 XM_010810275.3
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06979 XM_024999489.1
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa59105 XM_010810276.3
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa59105 XM_010817021.2
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa24264 XM_002691461.5
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa24264 XM_010816993.2
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa24265 XM_005193938.3
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa24265 XM_005213080.4
Latest version!
Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa06979 XM_010810275.2 Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa06978 XM_005213081.3 Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa24264 XM_002691461.4 Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa24265 XM_005213080.3 Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa59105 XM_010810276.2 Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa06978
    Clone ID Related Accession (Same CDS sequence) XM_005213081.3 , XM_005193939.3 , XM_005213081.4
    Accession Version XM_005213081.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213081.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTCAGAG AAGAGCTTTA TGGAATTCCA AGTCAAGTAT CATCTAGGCT GTCACAGCCA 
    TCTTCTCCTC ATTCGGGCTG CCCATCTCCT ACCATTCCAG CAGGTAAAGT CATTTCTTCA
    TCACAGAAGC ACAGCAAGAA GGCACTAAAG CAGGCACTAA AACAGCAACA ACAGAAGAAA
    CAGCAGCAGC AGCAATGCAG GCCAAGCATG TCCATCTCCT CCAACCAGCA CCTCTCTCTG
    AAGACTGTCA AAGCATCCAG TGACCCTGTA CCTGCCAAAC CTGCGATATG GGAAGGAAAG
    CAATCTGATG GACAGTCAAG CAGCCCTCAG AACTCAAACT CTAGCTTTTC TTCTTCTTCG
    GTTAAAGTGG AAAATCATTT GCTGGGCCTG GGGAAGAAGT CATTCCAGAG GTCTGACAGG
    CTCCACACAA GGCAAATGAA GAGAACTAAA TGTGCTGAGA TTGATGTTGA GACGCCAGAT
    TCCATTCTGG TTAATACAAA CCTACGAGCG CTAATCAACA AACACACCTT TTCAGTCCTT
    CCTGGAGACT GCCAACAGCG ATTGCTTTTA CTACTTCCAG AGGTGGATCG ACAGGTTGGT
    CCAGATGGTC TGATGAAGTT AAATGGCTCA GCCCTTAATA ATGAGTTCTT CACTTCAGCA
    GCCCAAGGCT GGAAGGAAAG ACTTTCAGAA GGTGAGTTTA CACCGGAGAT GCAGGTGAGA
    ATTCGACAAG AGATTGAGAA GGAGAAGAAA GTAGAGCCGT GGAAAGAACA ATTCTTTGAA
    AGCTACTATG GTCAGAGTTC TGGCCTGAGT CTTGAAGATT CAAAGAAATT GACAGCTTCA
    CCCAATGATC CCAAAGTAAA GAAAACCCCA GCTGAGCAAC CAAAATCTAC GCTTCCTTCA
    GAGGCCTCGC CTGTCAGTGT AGTCCCAGTA ATTCCCCAGT TAGAATCTAA AGAGGAAGTA
    CTGCAGATGC CATCACCAGT CAGAAAAGAA GAGCATGAAA GCCAAGATAA GACGCAGCCA
    CACTCCACAG AGCCCCTTCT TTCCCCAGCT ACCAATACAA ATGAGCTTAG CAGCATTCCT
    CCCATCAAGT GCCCGAAGGA TGAGGCTCTT GTGGAGCAAA AGCCAGTTGC CTCTGCTGAA
    CAGGAATCTG AGAAAAAGAA TCATCTCACT ACAGCTTCAA ATTATAATAA AAATGAAAGC
    CAGGAAGCTT TAGTTACTTC CCTGAGCAAA CCCAAGAGTC CTGGGGTAGA AACAACAGTA
    ATGAAGCCCA CAGTAGAAGC AAGTCCACAG GAGACAACTA TGAAAGAGCC TCCATCAACT
    CTGGCTGATC ACAGCCCAGA AAGCCTCAAG AGGAAATCTT CTCTCACCCA AGAAGAGGCC
    CCAACAAGCT GGGAGAAAAG GCCGCGTGTC ACTGAGCATC GCCAGCACCA GCAGCCATTT
    CAAGTCTCCC CACAGCCCTT TCTCAGTAGA GGGGACAGGT TCCAGGTGCG GAGAGTACCA
    CCTCTCAAGA TCCCGGTCTC CAGAATCTCT CCCATGCCGT TTCCTACATC GCAGGTCTCT
    CCCAGGGCTC GTTTTCCAAT CTCCATCACT AGTCCTAACA GAACAGGAGC CAGAACCCTT
    GCAGACATCA AAGCAAAAGC CCAGCTTGTC AAAGCACAGA GGGCTGCCGC CGCCGCCGCC
    GCCGCAGCCG CTGCTGCCGC CTCAGTTGGA GGGACCATTC CAGGACCTGG CCCAGGCGGT
    GGACAGGGTC CAGGGGAGGG GGGTGAAAGG AGAACTGCTA GAGGAGGGGA TCCTGACTCA
    GACAAAGTCA GTGAGCCCAG CAAGGGCCCC GCACTGGAAC TGGCAGGAAC TGGAAGCAGG
    GGAGGTACGA GAGAGCTTTT ACCCGCTGGG CCAGACACTC AGCCCCAGTC TGAGACCAGG
    ACCGCAGGCC AGCCACAGCC TCTTAGTGGC CCTGGAGCAC AACTACAGCA AACCCCCTCA
    GTGCCTCCCG CATCTGCCGT CGGTGGAGCA TGTGCAGGTG TCCCCTCCCC AGCCCACATT
    CATGCACTCC CGCCAGCTTT AGGAAAATTA AGTAATGAAA AACTAAATCC CCCCAGGGTA
    ACAGGCACGG TGGCCTCTCT CAGCCAACCC CAAGGGCCCA GTAATGGTAG GCAGGAGAAA
    GCACCTCCAG CTCCAGCAGA TCCTGCTCTG ATCATGGGTG CCTCGCCTGT TCATTTTGCA
    GCTGCTGGCG CAGTGGAGCC TAAAGCAGGT TCTAGTAAGA ATGCCCCCAA TCCTCCGGCC
    TCAGCAGAGA TAAGTGCTAG CGCCTCAGTG GATACAACTG CCTCCCCTTT AACATCTCTG
    TTAACAACAG CCACTTTAGA GAAGCTCCCT GTGCCGCAGG TCAGCGTAAC CACAACGCCT
    ACTGGATCAG CTCCATCCTT GAGCACTTTG CCGGCAGCCT CTAGCCTTAA AACTCCGGGA
    ACTTCTTCAC ATATGAATGG ACCCATTTCA AGGCCAAGCT CTAGTATCCC TGCTAATAAT
    CCTTTGGTAA CTCAGCTGCT GCAGGGCAAA GATGTTCCCA TGGAGCAAAT TCTGCCTAAA
    CCTCTTACCA AAGTTGAAAT GAAAACTGTT CCACTCACTA CAAAAGAGGA GAAGGGAGTA
    GGAGCACTCA TAGGTACCAG CGTGACAGAG AACAGCACCA GAGAGGAAGT TCATGAGAGA
    CAGTCCCATC CAGCTGCACA GCACCTGGGT AAAACTTTGC AAAGTAAGCA GCTCCCCCAG
    GTTCCAAGAC CCCTGCAGCT CTTTTCAGGT AAGGATCTAA GGGACTCTGG CATTGACACA
    CACCAGTACC AAGAAGGACT CAGTAAGGCC ACCCAAGATC AGATTCTTCA GACTCTCAGC
    CAGAGGGTTC GGAGGCAGAA CATTCTCTCA TTTGTGCAGC CCTCACAGTT CAACTTTACT
    CACTCAGGTT TCCCGTTAGA GGACATTTCC ACAAGCCAGA GGTTCATGCT AGGTTTTGCT
    GGCAGAAGGA CATCCAAGCC TGCGATGGCA GGTCACTACT TACTTAATAT TTCCACCTAT
    GGCCGGGGTA CAGAGAGCTT TAGGAGGACC CATTCTGTGA ACCCCGAAGA CCGATTTTGT
    CTGAGTAGCC CCACTGAGGC CTTGAAAATG GGATACACAG ACTGTAAAAA TGCAGCAGGA
    GATAGTAGCA GCGGGAAGGA AGATGATACT GACGAGGAAA GTACTGGTGA TGAGCAAGAA
    TCTGTCCCGG TAAAGGAGGA GCCACAGGCT TCTCAGAGTT CTGGCAAGTG TGATGCAAGT
    TTAGGACCCC ACAGTAGAGA AACCCTGTCC ACCAGTGACT GTTCCGCTAA AAAGAATGTG
    AAGGCAGAGA CACCAGTGCC TGAGCAAACC CCTTTAAGTA AGGAGAATTA CCTGTTCACT
    CGAGGCCAAA CCTTTGACGA GAAGACCCTA GCCAGAGATT TTATTCAGGC AGCACAGAAG
    CAGATGGCTC ATGCAGTGAG AGGTAAGACC ATGCGGAGCA GCCCAGAGCT TTTCAATTCT
    ACGTCTCTTC CTCTGCCTGC AGACAGTCCT ACCCATCAAT CTCTCCTCCT CCCACCACTG
    CAAACCCCAA AGCTGTACGG GAGCCCCACA CAGATCGGGC CAAGCTACAG AGGCATGATC
    AACGTCTCCA CCTCATCAGA CATGGACCAT AACTCCTCTG TCCCGGGGAT GCCCGACTGT
    AGTCAGGTGT CTAGCAATGT TGGTGATGTC ATGTCCTTTT CAGTGACTGT CACAGCCATC
    CCTGCCAGCC AGGCCGTGAA TGCCGGCAGC CGCGGCCAGA CCATTCCTGT TCAGGCCTTC
    CCTGAGGAGA GCAGCGTAGA GGACACTCCT TCCAAGTGTT ACTGCCGGTT GAAAGCCATG
    ATCATGTGCA AGGGCTGCGG AGCCTTCTGC CACGACGATT GCATTGGCCC CTCCAAACTC
    TGTGTCTCCT GCCTTGTTGT TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213138.1
    CDS983..4969
    Translation

    Target ORF information:

    RefSeq Version XM_005213081.3
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213081.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTCAGAG AAGAGCTTTA TGGAATTCCA AGTCAAGTAT CATCTAGGCT GTCACAGCCA 
    TCTTCTCCTC ATTCGGGCTG CCCATCTCCT ACCATTCCAG CAGGTAAAGT CATTTCTTCA
    TCACAGAAGC ACAGCAAGAA GGCACTAAAG CAGGCACTAA AACAGCAACA ACAGAAGAAA
    CAGCAGCAGC AGCAATGCAG GCCAAGCATG TCCATCTCCT CCAACCAGCA CCTCTCTCTG
    AAGACTGTCA AAGCATCCAG TGACCCTGTA CCTGCCAAAC CTGCGATATG GGAAGGAAAG
    CAATCTGATG GACAGTCAAG CAGCCCTCAG AACTCAAACT CTAGCTTTTC TTCTTCTTCG
    GTTAAAGTGG AAAATCATTT GCTGGGCCTG GGGAAGAAGT CATTCCAGAG GTCTGACAGG
    CTCCACACAA GGCAAATGAA GAGAACTAAA TGTGCTGAGA TTGATGTTGA GACGCCAGAT
    TCCATTCTGG TTAATACAAA CCTACGAGCG CTAATCAACA AACACACCTT TTCAGTCCTT
    CCTGGAGACT GCCAACAGCG ATTGCTTTTA CTACTTCCAG AGGTGGATCG ACAGGTTGGT
    CCAGATGGTC TGATGAAGTT AAATGGCTCA GCCCTTAATA ATGAGTTCTT CACTTCAGCA
    GCCCAAGGCT GGAAGGAAAG ACTTTCAGAA GGTGAGTTTA CACCGGAGAT GCAGGTGAGA
    ATTCGACAAG AGATTGAGAA GGAGAAGAAA GTAGAGCCGT GGAAAGAACA ATTCTTTGAA
    AGCTACTATG GTCAGAGTTC TGGCCTGAGT CTTGAAGATT CAAAGAAATT GACAGCTTCA
    CCCAATGATC CCAAAGTAAA GAAAACCCCA GCTGAGCAAC CAAAATCTAC GCTTCCTTCA
    GAGGCCTCGC CTGTCAGTGT AGTCCCAGTA ATTCCCCAGT TAGAATCTAA AGAGGAAGTA
    CTGCAGATGC CATCACCAGT CAGAAAAGAA GAGCATGAAA GCCAAGATAA GACGCAGCCA
    CACTCCACAG AGCCCCTTCT TTCCCCAGCT ACCAATACAA ATGAGCTTAG CAGCATTCCT
    CCCATCAAGT GCCCGAAGGA TGAGGCTCTT GTGGAGCAAA AGCCAGTTGC CTCTGCTGAA
    CAGGAATCTG AGAAAAAGAA TCATCTCACT ACAGCTTCAA ATTATAATAA AAATGAAAGC
    CAGGAAGCTT TAGTTACTTC CCTGAGCAAA CCCAAGAGTC CTGGGGTAGA AACAACAGTA
    ATGAAGCCCA CAGTAGAAGC AAGTCCACAG GAGACAACTA TGAAAGAGCC TCCATCAACT
    CTGGCTGATC ACAGCCCAGA AAGCCTCAAG AGGAAATCTT CTCTCACCCA AGAAGAGGCC
    CCAACAAGCT GGGAGAAAAG GCCGCGTGTC ACTGAGCATC GCCAGCACCA GCAGCCATTT
    CAAGTCTCCC CACAGCCCTT TCTCAGTAGA GGGGACAGGT TCCAGGTGCG GAGAGTACCA
    CCTCTCAAGA TCCCGGTCTC CAGAATCTCT CCCATGCCGT TTCCTACATC GCAGGTCTCT
    CCCAGGGCTC GTTTTCCAAT CTCCATCACT AGTCCTAACA GAACAGGAGC CAGAACCCTT
    GCAGACATCA AAGCAAAAGC CCAGCTTGTC AAAGCACAGA GGGCTGCCGC CGCCGCCGCC
    GCCGCAGCCG CTGCTGCCGC CTCAGTTGGA GGGACCATTC CAGGACCTGG CCCAGGCGGT
    GGACAGGGTC CAGGGGAGGG GGGTGAAAGG AGAACTGCTA GAGGAGGGGA TCCTGACTCA
    GACAAAGTCA GTGAGCCCAG CAAGGGCCCC GCACTGGAAC TGGCAGGAAC TGGAAGCAGG
    GGAGGTACGA GAGAGCTTTT ACCCGCTGGG CCAGACACTC AGCCCCAGTC TGAGACCAGG
    ACCGCAGGCC AGCCACAGCC TCTTAGTGGC CCTGGAGCAC AACTACAGCA AACCCCCTCA
    GTGCCTCCCG CATCTGCCGT CGGTGGAGCA TGTGCAGGTG TCCCCTCCCC AGCCCACATT
    CATGCACTCC CGCCAGCTTT AGGAAAATTA AGTAATGAAA AACTAAATCC CCCCAGGGTA
    ACAGGCACGG TGGCCTCTCT CAGCCAACCC CAAGGGCCCA GTAATGGTAG GCAGGAGAAA
    GCACCTCCAG CTCCAGCAGA TCCTGCTCTG ATCATGGGTG CCTCGCCTGT TCATTTTGCA
    GCTGCTGGCG CAGTGGAGCC TAAAGCAGGT TCTAGTAAGA ATGCCCCCAA TCCTCCGGCC
    TCAGCAGAGA TAAGTGCTAG CGCCTCAGTG GATACAACTG CCTCCCCTTT AACATCTCTG
    TTAACAACAG CCACTTTAGA GAAGCTCCCT GTGCCGCAGG TCAGCGTAAC CACAACGCCT
    ACTGGATCAG CTCCATCCTT GAGCACTTTG CCGGCAGCCT CTAGCCTTAA AACTCCGGGA
    ACTTCTTCAC ATATGAATGG ACCCATTTCA AGGCCAAGCT CTAGTATCCC TGCTAATAAT
    CCTTTGGTAA CTCAGCTGCT GCAGGGCAAA GATGTTCCCA TGGAGCAAAT TCTGCCTAAA
    CCTCTTACCA AAGTTGAAAT GAAAACTGTT CCACTCACTA CAAAAGAGGA GAAGGGAGTA
    GGAGCACTCA TAGGTACCAG CGTGACAGAG AACAGCACCA GAGAGGAAGT TCATGAGAGA
    CAGTCCCATC CAGCTGCACA GCACCTGGGT AAAACTTTGC AAAGTAAGCA GCTCCCCCAG
    GTTCCAAGAC CCCTGCAGCT CTTTTCAGGT AAGGATCTAA GGGACTCTGG CATTGACACA
    CACCAGTACC AAGAAGGACT CAGTAAGGCC ACCCAAGATC AGATTCTTCA GACTCTCAGC
    CAGAGGGTTC GGAGGCAGAA CATTCTCTCA TTTGTGCAGC CCTCACAGTT CAACTTTACT
    CACTCAGGTT TCCCGTTAGA GGACATTTCC ACAAGCCAGA GGTTCATGCT AGGTTTTGCT
    GGCAGAAGGA CATCCAAGCC TGCGATGGCA GGTCACTACT TACTTAATAT TTCCACCTAT
    GGCCGGGGTA CAGAGAGCTT TAGGAGGACC CATTCTGTGA ACCCCGAAGA CCGATTTTGT
    CTGAGTAGCC CCACTGAGGC CTTGAAAATG GGATACACAG ACTGTAAAAA TGCAGCAGGA
    GATAGTAGCA GCGGGAAGGA AGATGATACT GACGAGGAAA GTACTGGTGA TGAGCAAGAA
    TCTGTCCCGG TAAAGGAGGA GCCACAGGCT TCTCAGAGTT CTGGCAAGTG TGATGCAAGT
    TTAGGACCCC ACAGTAGAGA AACCCTGTCC ACCAGTGACT GTTCCGCTAA AAAGAATGTG
    AAGGCAGAGA CACCAGTGCC TGAGCAAACC CCTTTAAGTA AGGAGAATTA CCTGTTCACT
    CGAGGCCAAA CCTTTGACGA GAAGACCCTA GCCAGAGATT TTATTCAGGC AGCACAGAAG
    CAGATGGCTC ATGCAGTGAG AGGTAAGACC ATGCGGAGCA GCCCAGAGCT TTTCAATTCT
    ACGTCTCTTC CTCTGCCTGC AGACAGTCCT ACCCATCAAT CTCTCCTCCT CCCACCACTG
    CAAACCCCAA AGCTGTACGG GAGCCCCACA CAGATCGGGC CAAGCTACAG AGGCATGATC
    AACGTCTCCA CCTCATCAGA CATGGACCAT AACTCCTCTG TCCCGGGGAT GCCCGACTGT
    AGTCAGGTGT CTAGCAATGT TGGTGATGTC ATGTCCTTTT CAGTGACTGT CACAGCCATC
    CCTGCCAGCC AGGCCGTGAA TGCCGGCAGC CGCGGCCAGA CCATTCCTGT TCAGGCCTTC
    CCTGAGGAGA GCAGCGTAGA GGACACTCCT TCCAAGTGTT ACTGCCGGTT GAAAGCCATG
    ATCATGTGCA AGGGCTGCGG AGCCTTCTGC CACGACGATT GCATTGGCCC CTCCAAACTC
    TGTGTCTCCT GCCTTGTTGT TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06978
    Clone ID Related Accession (Same CDS sequence) XM_005213081.3 , XM_005193939.3 , XM_005213081.4
    Accession Version XM_005193939.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTCAGAG AAGAGCTTTA TGGAATTCCA AGTCAAGTAT CATCTAGGCT GTCACAGCCA 
    TCTTCTCCTC ATTCGGGCTG CCCATCTCCT ACCATTCCAG CAGGTAAAGT CATTTCTTCA
    TCACAGAAGC ACAGCAAGAA GGCACTAAAG CAGGCACTAA AACAGCAACA ACAGAAGAAA
    CAGCAGCAGC AGCAATGCAG GCCAAGCATG TCCATCTCCT CCAACCAGCA CCTCTCTCTG
    AAGACTGTCA AAGCATCCAG TGACCCTGTA CCTGCCAAAC CTGCGATATG GGAAGGAAAG
    CAATCTGATG GACAGTCAAG CAGCCCTCAG AACTCAAACT CTAGCTTTTC TTCTTCTTCG
    GTTAAAGTGG AAAATCATTT GCTGGGCCTG GGGAAGAAGT CATTCCAGAG GTCTGACAGG
    CTCCACACAA GGCAAATGAA GAGAACTAAA TGTGCTGAGA TTGATGTTGA GACGCCAGAT
    TCCATTCTGG TTAATACAAA CCTACGAGCG CTAATCAACA AACACACCTT TTCAGTCCTT
    CCTGGAGACT GCCAACAGCG ATTGCTTTTA CTACTTCCAG AGGTGGATCG ACAGGTTGGT
    CCAGATGGTC TGATGAAGTT AAATGGCTCA GCCCTTAATA ATGAGTTCTT CACTTCAGCA
    GCCCAAGGCT GGAAGGAAAG ACTTTCAGAA GGTGAGTTTA CACCGGAGAT GCAGGTGAGA
    ATTCGACAAG AGATTGAGAA GGAGAAGAAA GTAGAGCCGT GGAAAGAACA ATTCTTTGAA
    AGCTACTATG GTCAGAGTTC TGGCCTGAGT CTTGAAGATT CAAAGAAATT GACAGCTTCA
    CCCAATGATC CCAAAGTAAA GAAAACCCCA GCTGAGCAAC CAAAATCTAC GCTTCCTTCA
    GAGGCCTCGC CTGTCAGTGT AGTCCCAGTA ATTCCCCAGT TAGAATCTAA AGAGGAAGTA
    CTGCAGATGC CATCACCAGT CAGAAAAGAA GAGCATGAAA GCCAAGATAA GACGCAGCCA
    CACTCCACAG AGCCCCTTCT TTCCCCAGCT ACCAATACAA ATGAGCTTAG CAGCATTCCT
    CCCATCAAGT GCCCGAAGGA TGAGGCTCTT GTGGAGCAAA AGCCAGTTGC CTCTGCTGAA
    CAGGAATCTG AGAAAAAGAA TCATCTCACT ACAGCTTCAA ATTATAATAA AAATGAAAGC
    CAGGAAGCTT TAGTTACTTC CCTGAGCAAA CCCAAGAGTC CTGGGGTAGA AACAACAGTA
    ATGAAGCCCA CAGTAGAAGC AAGTCCACAG GAGACAACTA TGAAAGAGCC TCCATCAACT
    CTGGCTGATC ACAGCCCAGA AAGCCTCAAG AGGAAATCTT CTCTCACCCA AGAAGAGGCC
    CCAACAAGCT GGGAGAAAAG GCCGCGTGTC ACTGAGCATC GCCAGCACCA GCAGCCATTT
    CAAGTCTCCC CACAGCCCTT TCTCAGTAGA GGGGACAGGT TCCAGGTGCG GAGAGTACCA
    CCTCTCAAGA TCCCGGTCTC CAGAATCTCT CCCATGCCGT TTCCTACATC GCAGGTCTCT
    CCCAGGGCTC GTTTTCCAAT CTCCATCACT AGTCCTAACA GAACAGGAGC CAGAACCCTT
    GCAGACATCA AAGCAAAAGC CCAGCTTGTC AAAGCACAGA GGGCTGCCGC CGCCGCCGCC
    GCCGCAGCCG CTGCTGCCGC CTCAGTTGGA GGGACCATTC CAGGACCTGG CCCAGGCGGT
    GGACAGGGTC CAGGGGAGGG GGGTGAAAGG AGAACTGCTA GAGGAGGGGA TCCTGACTCA
    GACAAAGTCA GTGAGCCCAG CAAGGGCCCC GCACTGGAAC TGGCAGGAAC TGGAAGCAGG
    GGAGGTACGA GAGAGCTTTT ACCCGCTGGG CCAGACACTC AGCCCCAGTC TGAGACCAGG
    ACCGCAGGCC AGCCACAGCC TCTTAGTGGC CCTGGAGCAC AACTACAGCA AACCCCCTCA
    GTGCCTCCCG CATCTGCCGT CGGTGGAGCA TGTGCAGGTG TCCCCTCCCC AGCCCACATT
    CATGCACTCC CGCCAGCTTT AGGAAAATTA AGTAATGAAA AACTAAATCC CCCCAGGGTA
    ACAGGCACGG TGGCCTCTCT CAGCCAACCC CAAGGGCCCA GTAATGGTAG GCAGGAGAAA
    GCACCTCCAG CTCCAGCAGA TCCTGCTCTG ATCATGGGTG CCTCGCCTGT TCATTTTGCA
    GCTGCTGGCG CAGTGGAGCC TAAAGCAGGT TCTAGTAAGA ATGCCCCCAA TCCTCCGGCC
    TCAGCAGAGA TAAGTGCTAG CGCCTCAGTG GATACAACTG CCTCCCCTTT AACATCTCTG
    TTAACAACAG CCACTTTAGA GAAGCTCCCT GTGCCGCAGG TCAGCGTAAC CACAACGCCT
    ACTGGATCAG CTCCATCCTT GAGCACTTTG CCGGCAGCCT CTAGCCTTAA AACTCCGGGA
    ACTTCTTCAC ATATGAATGG ACCCATTTCA AGGCCAAGCT CTAGTATCCC TGCTAATAAT
    CCTTTGGTAA CTCAGCTGCT GCAGGGCAAA GATGTTCCCA TGGAGCAAAT TCTGCCTAAA
    CCTCTTACCA AAGTTGAAAT GAAAACTGTT CCACTCACTA CAAAAGAGGA GAAGGGAGTA
    GGAGCACTCA TAGGTACCAG CGTGACAGAG AACAGCACCA GAGAGGAAGT TCATGAGAGA
    CAGTCCCATC CAGCTGCACA GCACCTGGGT AAAACTTTGC AAAGTAAGCA GCTCCCCCAG
    GTTCCAAGAC CCCTGCAGCT CTTTTCAGGT AAGGATCTAA GGGACTCTGG CATTGACACA
    CACCAGTACC AAGAAGGACT CAGTAAGGCC ACCCAAGATC AGATTCTTCA GACTCTCAGC
    CAGAGGGTTC GGAGGCAGAA CATTCTCTCA TTTGTGCAGC CCTCACAGTT CAACTTTACT
    CACTCAGGTT TCCCGTTAGA GGACATTTCC ACAAGCCAGA GGTTCATGCT AGGTTTTGCT
    GGCAGAAGGA CATCCAAGCC TGCGATGGCA GGTCACTACT TACTTAATAT TTCCACCTAT
    GGCCGGGGTA CAGAGAGCTT TAGGAGGACC CATTCTGTGA ACCCCGAAGA CCGATTTTGT
    CTGAGTAGCC CCACTGAGGC CTTGAAAATG GGATACACAG ACTGTAAAAA TGCAGCAGGA
    GATAGTAGCA GCGGGAAGGA AGATGATACT GACGAGGAAA GTACTGGTGA TGAGCAAGAA
    TCTGTCCCGG TAAAGGAGGA GCCACAGGCT TCTCAGAGTT CTGGCAAGTG TGATGCAAGT
    TTAGGACCCC ACAGTAGAGA AACCCTGTCC ACCAGTGACT GTTCCGCTAA AAAGAATGTG
    AAGGCAGAGA CACCAGTGCC TGAGCAAACC CCTTTAAGTA AGGAGAATTA CCTGTTCACT
    CGAGGCCAAA CCTTTGACGA GAAGACCCTA GCCAGAGATT TTATTCAGGC AGCACAGAAG
    CAGATGGCTC ATGCAGTGAG AGGTAAGACC ATGCGGAGCA GCCCAGAGCT TTTCAATTCT
    ACGTCTCTTC CTCTGCCTGC AGACAGTCCT ACCCATCAAT CTCTCCTCCT CCCACCACTG
    CAAACCCCAA AGCTGTACGG GAGCCCCACA CAGATCGGGC CAAGCTACAG AGGCATGATC
    AACGTCTCCA CCTCATCAGA CATGGACCAT AACTCCTCTG TCCCGGGGAT GCCCGACTGT
    AGTCAGGTGT CTAGCAATGT TGGTGATGTC ATGTCCTTTT CAGTGACTGT CACAGCCATC
    CCTGCCAGCC AGGCCGTGAA TGCCGGCAGC CGCGGCCAGA CCATTCCTGT TCAGGCCTTC
    CCTGAGGAGA GCAGCGTAGA GGACACTCCT TCCAAGTGTT ACTGCCGGTT GAAAGCCATG
    ATCATGTGCA AGGGCTGCGG AGCCTTCTGC CACGACGATT GCATTGGCCC CTCCAAACTC
    TGTGTCTCCT GCCTTGTTGT TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193996.1
    CDS983..4969
    Translation

    Target ORF information:

    RefSeq Version XM_005193939.3
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193939.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTCAGAG AAGAGCTTTA TGGAATTCCA AGTCAAGTAT CATCTAGGCT GTCACAGCCA 
    TCTTCTCCTC ATTCGGGCTG CCCATCTCCT ACCATTCCAG CAGGTAAAGT CATTTCTTCA
    TCACAGAAGC ACAGCAAGAA GGCACTAAAG CAGGCACTAA AACAGCAACA ACAGAAGAAA
    CAGCAGCAGC AGCAATGCAG GCCAAGCATG TCCATCTCCT CCAACCAGCA CCTCTCTCTG
    AAGACTGTCA AAGCATCCAG TGACCCTGTA CCTGCCAAAC CTGCGATATG GGAAGGAAAG
    CAATCTGATG GACAGTCAAG CAGCCCTCAG AACTCAAACT CTAGCTTTTC TTCTTCTTCG
    GTTAAAGTGG AAAATCATTT GCTGGGCCTG GGGAAGAAGT CATTCCAGAG GTCTGACAGG
    CTCCACACAA GGCAAATGAA GAGAACTAAA TGTGCTGAGA TTGATGTTGA GACGCCAGAT
    TCCATTCTGG TTAATACAAA CCTACGAGCG CTAATCAACA AACACACCTT TTCAGTCCTT
    CCTGGAGACT GCCAACAGCG ATTGCTTTTA CTACTTCCAG AGGTGGATCG ACAGGTTGGT
    CCAGATGGTC TGATGAAGTT AAATGGCTCA GCCCTTAATA ATGAGTTCTT CACTTCAGCA
    GCCCAAGGCT GGAAGGAAAG ACTTTCAGAA GGTGAGTTTA CACCGGAGAT GCAGGTGAGA
    ATTCGACAAG AGATTGAGAA GGAGAAGAAA GTAGAGCCGT GGAAAGAACA ATTCTTTGAA
    AGCTACTATG GTCAGAGTTC TGGCCTGAGT CTTGAAGATT CAAAGAAATT GACAGCTTCA
    CCCAATGATC CCAAAGTAAA GAAAACCCCA GCTGAGCAAC CAAAATCTAC GCTTCCTTCA
    GAGGCCTCGC CTGTCAGTGT AGTCCCAGTA ATTCCCCAGT TAGAATCTAA AGAGGAAGTA
    CTGCAGATGC CATCACCAGT CAGAAAAGAA GAGCATGAAA GCCAAGATAA GACGCAGCCA
    CACTCCACAG AGCCCCTTCT TTCCCCAGCT ACCAATACAA ATGAGCTTAG CAGCATTCCT
    CCCATCAAGT GCCCGAAGGA TGAGGCTCTT GTGGAGCAAA AGCCAGTTGC CTCTGCTGAA
    CAGGAATCTG AGAAAAAGAA TCATCTCACT ACAGCTTCAA ATTATAATAA AAATGAAAGC
    CAGGAAGCTT TAGTTACTTC CCTGAGCAAA CCCAAGAGTC CTGGGGTAGA AACAACAGTA
    ATGAAGCCCA CAGTAGAAGC AAGTCCACAG GAGACAACTA TGAAAGAGCC TCCATCAACT
    CTGGCTGATC ACAGCCCAGA AAGCCTCAAG AGGAAATCTT CTCTCACCCA AGAAGAGGCC
    CCAACAAGCT GGGAGAAAAG GCCGCGTGTC ACTGAGCATC GCCAGCACCA GCAGCCATTT
    CAAGTCTCCC CACAGCCCTT TCTCAGTAGA GGGGACAGGT TCCAGGTGCG GAGAGTACCA
    CCTCTCAAGA TCCCGGTCTC CAGAATCTCT CCCATGCCGT TTCCTACATC GCAGGTCTCT
    CCCAGGGCTC GTTTTCCAAT CTCCATCACT AGTCCTAACA GAACAGGAGC CAGAACCCTT
    GCAGACATCA AAGCAAAAGC CCAGCTTGTC AAAGCACAGA GGGCTGCCGC CGCCGCCGCC
    GCCGCAGCCG CTGCTGCCGC CTCAGTTGGA GGGACCATTC CAGGACCTGG CCCAGGCGGT
    GGACAGGGTC CAGGGGAGGG GGGTGAAAGG AGAACTGCTA GAGGAGGGGA TCCTGACTCA
    GACAAAGTCA GTGAGCCCAG CAAGGGCCCC GCACTGGAAC TGGCAGGAAC TGGAAGCAGG
    GGAGGTACGA GAGAGCTTTT ACCCGCTGGG CCAGACACTC AGCCCCAGTC TGAGACCAGG
    ACCGCAGGCC AGCCACAGCC TCTTAGTGGC CCTGGAGCAC AACTACAGCA AACCCCCTCA
    GTGCCTCCCG CATCTGCCGT CGGTGGAGCA TGTGCAGGTG TCCCCTCCCC AGCCCACATT
    CATGCACTCC CGCCAGCTTT AGGAAAATTA AGTAATGAAA AACTAAATCC CCCCAGGGTA
    ACAGGCACGG TGGCCTCTCT CAGCCAACCC CAAGGGCCCA GTAATGGTAG GCAGGAGAAA
    GCACCTCCAG CTCCAGCAGA TCCTGCTCTG ATCATGGGTG CCTCGCCTGT TCATTTTGCA
    GCTGCTGGCG CAGTGGAGCC TAAAGCAGGT TCTAGTAAGA ATGCCCCCAA TCCTCCGGCC
    TCAGCAGAGA TAAGTGCTAG CGCCTCAGTG GATACAACTG CCTCCCCTTT AACATCTCTG
    TTAACAACAG CCACTTTAGA GAAGCTCCCT GTGCCGCAGG TCAGCGTAAC CACAACGCCT
    ACTGGATCAG CTCCATCCTT GAGCACTTTG CCGGCAGCCT CTAGCCTTAA AACTCCGGGA
    ACTTCTTCAC ATATGAATGG ACCCATTTCA AGGCCAAGCT CTAGTATCCC TGCTAATAAT
    CCTTTGGTAA CTCAGCTGCT GCAGGGCAAA GATGTTCCCA TGGAGCAAAT TCTGCCTAAA
    CCTCTTACCA AAGTTGAAAT GAAAACTGTT CCACTCACTA CAAAAGAGGA GAAGGGAGTA
    GGAGCACTCA TAGGTACCAG CGTGACAGAG AACAGCACCA GAGAGGAAGT TCATGAGAGA
    CAGTCCCATC CAGCTGCACA GCACCTGGGT AAAACTTTGC AAAGTAAGCA GCTCCCCCAG
    GTTCCAAGAC CCCTGCAGCT CTTTTCAGGT AAGGATCTAA GGGACTCTGG CATTGACACA
    CACCAGTACC AAGAAGGACT CAGTAAGGCC ACCCAAGATC AGATTCTTCA GACTCTCAGC
    CAGAGGGTTC GGAGGCAGAA CATTCTCTCA TTTGTGCAGC CCTCACAGTT CAACTTTACT
    CACTCAGGTT TCCCGTTAGA GGACATTTCC ACAAGCCAGA GGTTCATGCT AGGTTTTGCT
    GGCAGAAGGA CATCCAAGCC TGCGATGGCA GGTCACTACT TACTTAATAT TTCCACCTAT
    GGCCGGGGTA CAGAGAGCTT TAGGAGGACC CATTCTGTGA ACCCCGAAGA CCGATTTTGT
    CTGAGTAGCC CCACTGAGGC CTTGAAAATG GGATACACAG ACTGTAAAAA TGCAGCAGGA
    GATAGTAGCA GCGGGAAGGA AGATGATACT GACGAGGAAA GTACTGGTGA TGAGCAAGAA
    TCTGTCCCGG TAAAGGAGGA GCCACAGGCT TCTCAGAGTT CTGGCAAGTG TGATGCAAGT
    TTAGGACCCC ACAGTAGAGA AACCCTGTCC ACCAGTGACT GTTCCGCTAA AAAGAATGTG
    AAGGCAGAGA CACCAGTGCC TGAGCAAACC CCTTTAAGTA AGGAGAATTA CCTGTTCACT
    CGAGGCCAAA CCTTTGACGA GAAGACCCTA GCCAGAGATT TTATTCAGGC AGCACAGAAG
    CAGATGGCTC ATGCAGTGAG AGGTAAGACC ATGCGGAGCA GCCCAGAGCT TTTCAATTCT
    ACGTCTCTTC CTCTGCCTGC AGACAGTCCT ACCCATCAAT CTCTCCTCCT CCCACCACTG
    CAAACCCCAA AGCTGTACGG GAGCCCCACA CAGATCGGGC CAAGCTACAG AGGCATGATC
    AACGTCTCCA CCTCATCAGA CATGGACCAT AACTCCTCTG TCCCGGGGAT GCCCGACTGT
    AGTCAGGTGT CTAGCAATGT TGGTGATGTC ATGTCCTTTT CAGTGACTGT CACAGCCATC
    CCTGCCAGCC AGGCCGTGAA TGCCGGCAGC CGCGGCCAGA CCATTCCTGT TCAGGCCTTC
    CCTGAGGAGA GCAGCGTAGA GGACACTCCT TCCAAGTGTT ACTGCCGGTT GAAAGCCATG
    ATCATGTGCA AGGGCTGCGG AGCCTTCTGC CACGACGATT GCATTGGCCC CTCCAAACTC
    TGTGTCTCCT GCCTTGTTGT TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06978
    Clone ID Related Accession (Same CDS sequence) XM_005213081.3 , XM_005193939.3 , XM_005213081.4
    Accession Version XM_005213081.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213081.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTCAGAG AAGAGCTTTA TGGAATTCCA AGTCAAGTAT CATCTAGGCT GTCACAGCCA 
    TCTTCTCCTC ATTCGGGCTG CCCATCTCCT ACCATTCCAG CAGGTAAAGT CATTTCTTCA
    TCACAGAAGC ACAGCAAGAA GGCACTAAAG CAGGCACTAA AACAGCAACA ACAGAAGAAA
    CAGCAGCAGC AGCAATGCAG GCCAAGCATG TCCATCTCCT CCAACCAGCA CCTCTCTCTG
    AAGACTGTCA AAGCATCCAG TGACCCTGTA CCTGCCAAAC CTGCGATATG GGAAGGAAAG
    CAATCTGATG GACAGTCAAG CAGCCCTCAG AACTCAAACT CTAGCTTTTC TTCTTCTTCG
    GTTAAAGTGG AAAATCATTT GCTGGGCCTG GGGAAGAAGT CATTCCAGAG GTCTGACAGG
    CTCCACACAA GGCAAATGAA GAGAACTAAA TGTGCTGAGA TTGATGTTGA GACGCCAGAT
    TCCATTCTGG TTAATACAAA CCTACGAGCG CTAATCAACA AACACACCTT TTCAGTCCTT
    CCTGGAGACT GCCAACAGCG ATTGCTTTTA CTACTTCCAG AGGTGGATCG ACAGGTTGGT
    CCAGATGGTC TGATGAAGTT AAATGGCTCA GCCCTTAATA ATGAGTTCTT CACTTCAGCA
    GCCCAAGGCT GGAAGGAAAG ACTTTCAGAA GGTGAGTTTA CACCGGAGAT GCAGGTGAGA
    ATTCGACAAG AGATTGAGAA GGAGAAGAAA GTAGAGCCGT GGAAAGAACA ATTCTTTGAA
    AGCTACTATG GTCAGAGTTC TGGCCTGAGT CTTGAAGATT CAAAGAAATT GACAGCTTCA
    CCCAATGATC CCAAAGTAAA GAAAACCCCA GCTGAGCAAC CAAAATCTAC GCTTCCTTCA
    GAGGCCTCGC CTGTCAGTGT AGTCCCAGTA ATTCCCCAGT TAGAATCTAA AGAGGAAGTA
    CTGCAGATGC CATCACCAGT CAGAAAAGAA GAGCATGAAA GCCAAGATAA GACGCAGCCA
    CACTCCACAG AGCCCCTTCT TTCCCCAGCT ACCAATACAA ATGAGCTTAG CAGCATTCCT
    CCCATCAAGT GCCCGAAGGA TGAGGCTCTT GTGGAGCAAA AGCCAGTTGC CTCTGCTGAA
    CAGGAATCTG AGAAAAAGAA TCATCTCACT ACAGCTTCAA ATTATAATAA AAATGAAAGC
    CAGGAAGCTT TAGTTACTTC CCTGAGCAAA CCCAAGAGTC CTGGGGTAGA AACAACAGTA
    ATGAAGCCCA CAGTAGAAGC AAGTCCACAG GAGACAACTA TGAAAGAGCC TCCATCAACT
    CTGGCTGATC ACAGCCCAGA AAGCCTCAAG AGGAAATCTT CTCTCACCCA AGAAGAGGCC
    CCAACAAGCT GGGAGAAAAG GCCGCGTGTC ACTGAGCATC GCCAGCACCA GCAGCCATTT
    CAAGTCTCCC CACAGCCCTT TCTCAGTAGA GGGGACAGGT TCCAGGTGCG GAGAGTACCA
    CCTCTCAAGA TCCCGGTCTC CAGAATCTCT CCCATGCCGT TTCCTACATC GCAGGTCTCT
    CCCAGGGCTC GTTTTCCAAT CTCCATCACT AGTCCTAACA GAACAGGAGC CAGAACCCTT
    GCAGACATCA AAGCAAAAGC CCAGCTTGTC AAAGCACAGA GGGCTGCCGC CGCCGCCGCC
    GCCGCAGCCG CTGCTGCCGC CTCAGTTGGA GGGACCATTC CAGGACCTGG CCCAGGCGGT
    GGACAGGGTC CAGGGGAGGG GGGTGAAAGG AGAACTGCTA GAGGAGGGGA TCCTGACTCA
    GACAAAGTCA GTGAGCCCAG CAAGGGCCCC GCACTGGAAC TGGCAGGAAC TGGAAGCAGG
    GGAGGTACGA GAGAGCTTTT ACCCGCTGGG CCAGACACTC AGCCCCAGTC TGAGACCAGG
    ACCGCAGGCC AGCCACAGCC TCTTAGTGGC CCTGGAGCAC AACTACAGCA AACCCCCTCA
    GTGCCTCCCG CATCTGCCGT CGGTGGAGCA TGTGCAGGTG TCCCCTCCCC AGCCCACATT
    CATGCACTCC CGCCAGCTTT AGGAAAATTA AGTAATGAAA AACTAAATCC CCCCAGGGTA
    ACAGGCACGG TGGCCTCTCT CAGCCAACCC CAAGGGCCCA GTAATGGTAG GCAGGAGAAA
    GCACCTCCAG CTCCAGCAGA TCCTGCTCTG ATCATGGGTG CCTCGCCTGT TCATTTTGCA
    GCTGCTGGCG CAGTGGAGCC TAAAGCAGGT TCTAGTAAGA ATGCCCCCAA TCCTCCGGCC
    TCAGCAGAGA TAAGTGCTAG CGCCTCAGTG GATACAACTG CCTCCCCTTT AACATCTCTG
    TTAACAACAG CCACTTTAGA GAAGCTCCCT GTGCCGCAGG TCAGCGTAAC CACAACGCCT
    ACTGGATCAG CTCCATCCTT GAGCACTTTG CCGGCAGCCT CTAGCCTTAA AACTCCGGGA
    ACTTCTTCAC ATATGAATGG ACCCATTTCA AGGCCAAGCT CTAGTATCCC TGCTAATAAT
    CCTTTGGTAA CTCAGCTGCT GCAGGGCAAA GATGTTCCCA TGGAGCAAAT TCTGCCTAAA
    CCTCTTACCA AAGTTGAAAT GAAAACTGTT CCACTCACTA CAAAAGAGGA GAAGGGAGTA
    GGAGCACTCA TAGGTACCAG CGTGACAGAG AACAGCACCA GAGAGGAAGT TCATGAGAGA
    CAGTCCCATC CAGCTGCACA GCACCTGGGT AAAACTTTGC AAAGTAAGCA GCTCCCCCAG
    GTTCCAAGAC CCCTGCAGCT CTTTTCAGGT AAGGATCTAA GGGACTCTGG CATTGACACA
    CACCAGTACC AAGAAGGACT CAGTAAGGCC ACCCAAGATC AGATTCTTCA GACTCTCAGC
    CAGAGGGTTC GGAGGCAGAA CATTCTCTCA TTTGTGCAGC CCTCACAGTT CAACTTTACT
    CACTCAGGTT TCCCGTTAGA GGACATTTCC ACAAGCCAGA GGTTCATGCT AGGTTTTGCT
    GGCAGAAGGA CATCCAAGCC TGCGATGGCA GGTCACTACT TACTTAATAT TTCCACCTAT
    GGCCGGGGTA CAGAGAGCTT TAGGAGGACC CATTCTGTGA ACCCCGAAGA CCGATTTTGT
    CTGAGTAGCC CCACTGAGGC CTTGAAAATG GGATACACAG ACTGTAAAAA TGCAGCAGGA
    GATAGTAGCA GCGGGAAGGA AGATGATACT GACGAGGAAA GTACTGGTGA TGAGCAAGAA
    TCTGTCCCGG TAAAGGAGGA GCCACAGGCT TCTCAGAGTT CTGGCAAGTG TGATGCAAGT
    TTAGGACCCC ACAGTAGAGA AACCCTGTCC ACCAGTGACT GTTCCGCTAA AAAGAATGTG
    AAGGCAGAGA CACCAGTGCC TGAGCAAACC CCTTTAAGTA AGGAGAATTA CCTGTTCACT
    CGAGGCCAAA CCTTTGACGA GAAGACCCTA GCCAGAGATT TTATTCAGGC AGCACAGAAG
    CAGATGGCTC ATGCAGTGAG AGGTAAGACC ATGCGGAGCA GCCCAGAGCT TTTCAATTCT
    ACGTCTCTTC CTCTGCCTGC AGACAGTCCT ACCCATCAAT CTCTCCTCCT CCCACCACTG
    CAAACCCCAA AGCTGTACGG GAGCCCCACA CAGATCGGGC CAAGCTACAG AGGCATGATC
    AACGTCTCCA CCTCATCAGA CATGGACCAT AACTCCTCTG TCCCGGGGAT GCCCGACTGT
    AGTCAGGTGT CTAGCAATGT TGGTGATGTC ATGTCCTTTT CAGTGACTGT CACAGCCATC
    CCTGCCAGCC AGGCCGTGAA TGCCGGCAGC CGCGGCCAGA CCATTCCTGT TCAGGCCTTC
    CCTGAGGAGA GCAGCGTAGA GGACACTCCT TCCAAGTGTT ACTGCCGGTT GAAAGCCATG
    ATCATGTGCA AGGGCTGCGG AGCCTTCTGC CACGACGATT GCATTGGCCC CTCCAAACTC
    TGTGTCTCCT GCCTTGTTGT TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213138.1
    CDS218..4204
    Translation

    Target ORF information:

    RefSeq Version XM_005213081.4
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213081.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTCAGAG AAGAGCTTTA TGGAATTCCA AGTCAAGTAT CATCTAGGCT GTCACAGCCA 
    TCTTCTCCTC ATTCGGGCTG CCCATCTCCT ACCATTCCAG CAGGTAAAGT CATTTCTTCA
    TCACAGAAGC ACAGCAAGAA GGCACTAAAG CAGGCACTAA AACAGCAACA ACAGAAGAAA
    CAGCAGCAGC AGCAATGCAG GCCAAGCATG TCCATCTCCT CCAACCAGCA CCTCTCTCTG
    AAGACTGTCA AAGCATCCAG TGACCCTGTA CCTGCCAAAC CTGCGATATG GGAAGGAAAG
    CAATCTGATG GACAGTCAAG CAGCCCTCAG AACTCAAACT CTAGCTTTTC TTCTTCTTCG
    GTTAAAGTGG AAAATCATTT GCTGGGCCTG GGGAAGAAGT CATTCCAGAG GTCTGACAGG
    CTCCACACAA GGCAAATGAA GAGAACTAAA TGTGCTGAGA TTGATGTTGA GACGCCAGAT
    TCCATTCTGG TTAATACAAA CCTACGAGCG CTAATCAACA AACACACCTT TTCAGTCCTT
    CCTGGAGACT GCCAACAGCG ATTGCTTTTA CTACTTCCAG AGGTGGATCG ACAGGTTGGT
    CCAGATGGTC TGATGAAGTT AAATGGCTCA GCCCTTAATA ATGAGTTCTT CACTTCAGCA
    GCCCAAGGCT GGAAGGAAAG ACTTTCAGAA GGTGAGTTTA CACCGGAGAT GCAGGTGAGA
    ATTCGACAAG AGATTGAGAA GGAGAAGAAA GTAGAGCCGT GGAAAGAACA ATTCTTTGAA
    AGCTACTATG GTCAGAGTTC TGGCCTGAGT CTTGAAGATT CAAAGAAATT GACAGCTTCA
    CCCAATGATC CCAAAGTAAA GAAAACCCCA GCTGAGCAAC CAAAATCTAC GCTTCCTTCA
    GAGGCCTCGC CTGTCAGTGT AGTCCCAGTA ATTCCCCAGT TAGAATCTAA AGAGGAAGTA
    CTGCAGATGC CATCACCAGT CAGAAAAGAA GAGCATGAAA GCCAAGATAA GACGCAGCCA
    CACTCCACAG AGCCCCTTCT TTCCCCAGCT ACCAATACAA ATGAGCTTAG CAGCATTCCT
    CCCATCAAGT GCCCGAAGGA TGAGGCTCTT GTGGAGCAAA AGCCAGTTGC CTCTGCTGAA
    CAGGAATCTG AGAAAAAGAA TCATCTCACT ACAGCTTCAA ATTATAATAA AAATGAAAGC
    CAGGAAGCTT TAGTTACTTC CCTGAGCAAA CCCAAGAGTC CTGGGGTAGA AACAACAGTA
    ATGAAGCCCA CAGTAGAAGC AAGTCCACAG GAGACAACTA TGAAAGAGCC TCCATCAACT
    CTGGCTGATC ACAGCCCAGA AAGCCTCAAG AGGAAATCTT CTCTCACCCA AGAAGAGGCC
    CCAACAAGCT GGGAGAAAAG GCCGCGTGTC ACTGAGCATC GCCAGCACCA GCAGCCATTT
    CAAGTCTCCC CACAGCCCTT TCTCAGTAGA GGGGACAGGT TCCAGGTGCG GAGAGTACCA
    CCTCTCAAGA TCCCGGTCTC CAGAATCTCT CCCATGCCGT TTCCTACATC GCAGGTCTCT
    CCCAGGGCTC GTTTTCCAAT CTCCATCACT AGTCCTAACA GAACAGGAGC CAGAACCCTT
    GCAGACATCA AAGCAAAAGC CCAGCTTGTC AAAGCACAGA GGGCTGCCGC CGCCGCCGCC
    GCCGCAGCCG CTGCTGCCGC CTCAGTTGGA GGGACCATTC CAGGACCTGG CCCAGGCGGT
    GGACAGGGTC CAGGGGAGGG GGGTGAAAGG AGAACTGCTA GAGGAGGGGA TCCTGACTCA
    GACAAAGTCA GTGAGCCCAG CAAGGGCCCC GCACTGGAAC TGGCAGGAAC TGGAAGCAGG
    GGAGGTACGA GAGAGCTTTT ACCCGCTGGG CCAGACACTC AGCCCCAGTC TGAGACCAGG
    ACCGCAGGCC AGCCACAGCC TCTTAGTGGC CCTGGAGCAC AACTACAGCA AACCCCCTCA
    GTGCCTCCCG CATCTGCCGT CGGTGGAGCA TGTGCAGGTG TCCCCTCCCC AGCCCACATT
    CATGCACTCC CGCCAGCTTT AGGAAAATTA AGTAATGAAA AACTAAATCC CCCCAGGGTA
    ACAGGCACGG TGGCCTCTCT CAGCCAACCC CAAGGGCCCA GTAATGGTAG GCAGGAGAAA
    GCACCTCCAG CTCCAGCAGA TCCTGCTCTG ATCATGGGTG CCTCGCCTGT TCATTTTGCA
    GCTGCTGGCG CAGTGGAGCC TAAAGCAGGT TCTAGTAAGA ATGCCCCCAA TCCTCCGGCC
    TCAGCAGAGA TAAGTGCTAG CGCCTCAGTG GATACAACTG CCTCCCCTTT AACATCTCTG
    TTAACAACAG CCACTTTAGA GAAGCTCCCT GTGCCGCAGG TCAGCGTAAC CACAACGCCT
    ACTGGATCAG CTCCATCCTT GAGCACTTTG CCGGCAGCCT CTAGCCTTAA AACTCCGGGA
    ACTTCTTCAC ATATGAATGG ACCCATTTCA AGGCCAAGCT CTAGTATCCC TGCTAATAAT
    CCTTTGGTAA CTCAGCTGCT GCAGGGCAAA GATGTTCCCA TGGAGCAAAT TCTGCCTAAA
    CCTCTTACCA AAGTTGAAAT GAAAACTGTT CCACTCACTA CAAAAGAGGA GAAGGGAGTA
    GGAGCACTCA TAGGTACCAG CGTGACAGAG AACAGCACCA GAGAGGAAGT TCATGAGAGA
    CAGTCCCATC CAGCTGCACA GCACCTGGGT AAAACTTTGC AAAGTAAGCA GCTCCCCCAG
    GTTCCAAGAC CCCTGCAGCT CTTTTCAGGT AAGGATCTAA GGGACTCTGG CATTGACACA
    CACCAGTACC AAGAAGGACT CAGTAAGGCC ACCCAAGATC AGATTCTTCA GACTCTCAGC
    CAGAGGGTTC GGAGGCAGAA CATTCTCTCA TTTGTGCAGC CCTCACAGTT CAACTTTACT
    CACTCAGGTT TCCCGTTAGA GGACATTTCC ACAAGCCAGA GGTTCATGCT AGGTTTTGCT
    GGCAGAAGGA CATCCAAGCC TGCGATGGCA GGTCACTACT TACTTAATAT TTCCACCTAT
    GGCCGGGGTA CAGAGAGCTT TAGGAGGACC CATTCTGTGA ACCCCGAAGA CCGATTTTGT
    CTGAGTAGCC CCACTGAGGC CTTGAAAATG GGATACACAG ACTGTAAAAA TGCAGCAGGA
    GATAGTAGCA GCGGGAAGGA AGATGATACT GACGAGGAAA GTACTGGTGA TGAGCAAGAA
    TCTGTCCCGG TAAAGGAGGA GCCACAGGCT TCTCAGAGTT CTGGCAAGTG TGATGCAAGT
    TTAGGACCCC ACAGTAGAGA AACCCTGTCC ACCAGTGACT GTTCCGCTAA AAAGAATGTG
    AAGGCAGAGA CACCAGTGCC TGAGCAAACC CCTTTAAGTA AGGAGAATTA CCTGTTCACT
    CGAGGCCAAA CCTTTGACGA GAAGACCCTA GCCAGAGATT TTATTCAGGC AGCACAGAAG
    CAGATGGCTC ATGCAGTGAG AGGTAAGACC ATGCGGAGCA GCCCAGAGCT TTTCAATTCT
    ACGTCTCTTC CTCTGCCTGC AGACAGTCCT ACCCATCAAT CTCTCCTCCT CCCACCACTG
    CAAACCCCAA AGCTGTACGG GAGCCCCACA CAGATCGGGC CAAGCTACAG AGGCATGATC
    AACGTCTCCA CCTCATCAGA CATGGACCAT AACTCCTCTG TCCCGGGGAT GCCCGACTGT
    AGTCAGGTGT CTAGCAATGT TGGTGATGTC ATGTCCTTTT CAGTGACTGT CACAGCCATC
    CCTGCCAGCC AGGCCGTGAA TGCCGGCAGC CGCGGCCAGA CCATTCCTGT TCAGGCCTTC
    CCTGAGGAGA GCAGCGTAGA GGACACTCCT TCCAAGTGTT ACTGCCGGTT GAAAGCCATG
    ATCATGTGCA AGGGCTGCGG AGCCTTCTGC CACGACGATT GCATTGGCCC CTCCAAACTC
    TGTGTCTCCT GCCTTGTTGT TCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06979
    Clone ID Related Accession (Same CDS sequence) XM_010817016.2 , XM_010810275.3 , XM_010810275.2 , XM_024999489.1
    Accession Version XM_010817016.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCATCT CCTCCAACCA GCACCTCTCT CTGAAGACTG TCAAAGCATC CAGTGACCCT 
    GTACCTGCCA AACCTGCGAT ATGGGAAGGA AAGCAATCTG ATGGACAGTC AAGCAGCCCT
    CAGAACTCAA ACTCTAGCTT TTCTTCTTCT TCGGTTAAAG TGGAAAATCA TTTGCTGGGC
    CTGGGGAAGA AGTCATTCCA GAGGTCTGAC AGGCTCCACA CAAGGCAAAT GAAGAGAACT
    AAATGTGCTG AGATTGATGT TGAGACGCCA GATTCCATTC TGGTTAATAC AAACCTACGA
    GCGCTAATCA ACAAACACAC CTTTTCAGTC CTTCCTGGAG ACTGCCAACA GCGATTGCTT
    TTACTACTTC CAGAGGTGGA TCGACAGGTT GGTCCAGATG GTCTGATGAA GTTAAATGGC
    TCAGCCCTTA ATAATGAGTT CTTCACTTCA GCAGCCCAAG GCTGGAAGGA AAGACTTTCA
    GAAGGTGAGT TTACACCGGA GATGCAGGTG AGAATTCGAC AAGAGATTGA GAAGGAGAAG
    AAAGTAGAGC CGTGGAAAGA ACAATTCTTT GAAAGCTACT ATGGTCAGAG TTCTGGCCTG
    AGTCTTGAAG ATTCAAAGAA ATTGACAGCT TCACCCAATG ATCCCAAAGT AAAGAAAACC
    CCAGCTGAGC AACCAAAATC TACGCTTCCT TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA
    GTAATTCCCC AGTTAGAATC TAAAGAGGAA GTACTGCAGA TGCCATCACC AGTCAGAAAA
    GAAGAGCATG AAAGCCAAGA TAAGACGCAG CCACACTCCA CAGAGCCCCT TCTTTCCCCA
    GCTACCAATA CAAATGAGCT TAGCAGCATT CCTCCCATCA AGTGCCCGAA GGATGAGGCT
    CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT GAACAGGAAT CTGAGAAAAA GAATCATCTC
    ACTACAGCTT CAAATTATAA TAAAAATGAA AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC
    AAACCCAAGA GTCCTGGGGT AGAAACAACA GTAATGAAGC CCACAGTAGA AGCAAGTCCA
    CAGGAGACAA CTATGAAAGA GCCTCCATCA ACTCTGGCTG ATCACAGCCC AGAAAGCCTC
    AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG GCCCCAACAA GCTGGGAGAA AAGGCCGCGT
    GTCACTGAGC ATCGCCAGCA CCAGCAGCCA TTTCAAGTCT CCCCACAGCC CTTTCTCAGT
    AGAGGGGACA GGTTCCAGGT GCGGAGAGTA CCACCTCTCA AGATCCCGGT CTCCAGAATC
    TCTCCCATGC CGTTTCCTAC ATCGCAGGTC TCTCCCAGGG CTCGTTTTCC AATCTCCATC
    ACTAGTCCTA ACAGAACAGG AGCCAGAACC CTTGCAGACA TCAAAGCAAA AGCCCAGCTT
    GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT
    GGAGGGACCA TTCCAGGACC TGGCCCAGGC GGTGGACAGG GTCCAGGGGA GGGGGGTGAA
    AGGAGAACTG CTAGAGGAGG GGATCCTGAC TCAGACAAAG TCAGTGAGCC CAGCAAGGGC
    CCCGCACTGG AACTGGCAGG AACTGGAAGC AGGGGAGGTA CGAGAGAGCT TTTACCCGCT
    GGGCCAGACA CTCAGCCCCA GTCTGAGACC AGGACCGCAG GCCAGCCACA GCCTCTTAGT
    GGCCCTGGAG CACAACTACA GCAAACCCCC TCAGTGCCTC CCGCATCTGC CGTCGGTGGA
    GCATGTGCAG GTGTCCCCTC CCCAGCCCAC ATTCATGCAC TCCCGCCAGC TTTAGGAAAA
    TTAAGTAATG AAAAACTAAA TCCCCCCAGG GTAACAGGCA CGGTGGCCTC TCTCAGCCAA
    CCCCAAGGGC CCAGTAATGG TAGGCAGGAG AAAGCACCTC CAGCTCCAGC AGATCCTGCT
    CTGATCATGG GTGCCTCGCC TGTTCATTTT GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA
    GGTTCTAGTA AGAATGCCCC CAATCCTCCG GCCTCAGCAG AGATAAGTGC TAGCGCCTCA
    GTGGATACAA CTGCCTCCCC TTTAACATCT CTGTTAACAA CAGCCACTTT AGAGAAGCTC
    CCTGTGCCGC AGGTCAGCGT AACCACAACG CCTACTGGAT CAGCTCCATC CTTGAGCACT
    TTGCCGGCAG CCTCTAGCCT TAAAACTCCG GGAACTTCTT CACATATGAA TGGACCCATT
    TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT AATCCTTTGG TAACTCAGCT GCTGCAGGGC
    AAAGATGTTC CCATGGAGCA AATTCTGCCT AAACCTCTTA CCAAAGTTGA AATGAAAACT
    GTTCCACTCA CTACAAAAGA GGAGAAGGGA GTAGGAGCAC TCATAGGTAC CAGCGTGACA
    GAGAACAGCA CCAGAGAGGA AGTTCATGAG AGACAGTCCC ATCCAGCTGC ACAGCACCTG
    GGTAAAACTT TGCAAAGTAA GCAGCTCCCC CAGGTTCCAA GACCCCTGCA GCTCTTTTCA
    GGTAAGGATC TAAGGGACTC TGGCATTGAC ACACACCAGT ACCAAGAAGG ACTCAGTAAG
    GCCACCCAAG ATCAGATTCT TCAGACTCTC AGCCAGAGGG TTCGGAGGCA GAACATTCTC
    TCATTTGTGC AGCCCTCACA GTTCAACTTT ACTCACTCAG GTTTCCCGTT AGAGGACATT
    TCCACAAGCC AGAGGTTCAT GCTAGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCGATG
    GCAGGTCACT ACTTACTTAA TATTTCCACC TATGGCCGGG GTACAGAGAG CTTTAGGAGG
    ACCCATTCTG TGAACCCCGA AGACCGATTT TGTCTGAGTA GCCCCACTGA GGCCTTGAAA
    ATGGGATACA CAGACTGTAA AAATGCAGCA GGAGATAGTA GCAGCGGGAA GGAAGATGAT
    ACTGACGAGG AAAGTACTGG TGATGAGCAA GAATCTGTCC CGGTAAAGGA GGAGCCACAG
    GCTTCTCAGA GTTCTGGCAA GTGTGATGCA AGTTTAGGAC CCCACAGTAG AGAAACCCTG
    TCCACCAGTG ACTGTTCCGC TAAAAAGAAT GTGAAGGCAG AGACACCAGT GCCTGAGCAA
    ACCCCTTTAA GTAAGGAGAA TTACCTGTTC ACTCGAGGCC AAACCTTTGA CGAGAAGACC
    CTAGCCAGAG ATTTTATTCA GGCAGCACAG AAGCAGATGG CTCATGCAGT GAGAGGTAAG
    ACCATGCGGA GCAGCCCAGA GCTTTTCAAT TCTACGTCTC TTCCTCTGCC TGCAGACAGT
    CCTACCCATC AATCTCTCCT CCTCCCACCA CTGCAAACCC CAAAGCTGTA CGGGAGCCCC
    ACACAGATCG GGCCAAGCTA CAGAGGCATG ATCAACGTCT CCACCTCATC AGACATGGAC
    CATAACTCCT CTGTCCCGGG GATGCCCGAC TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT
    GTCATGTCCT TTTCAGTGAC TGTCACAGCC ATCCCTGCCA GCCAGGCCGT GAATGCCGGC
    AGCCGCGGCC AGACCATTCC TGTTCAGGCC TTCCCTGAGG AGAGCAGCGT AGAGGACACT
    CCTTCCAAGT GTTACTGCCG GTTGAAAGCC ATGATCATGT GCAAGGGCTG CGGAGCCTTC
    TGCCACGACG ATTGCATTGG CCCCTCCAAA CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815318.1
    CDS275..4054
    Translation

    Target ORF information:

    RefSeq Version XM_010817016.2
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817016.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCATCT CCTCCAACCA GCACCTCTCT CTGAAGACTG TCAAAGCATC CAGTGACCCT 
    GTACCTGCCA AACCTGCGAT ATGGGAAGGA AAGCAATCTG ATGGACAGTC AAGCAGCCCT
    CAGAACTCAA ACTCTAGCTT TTCTTCTTCT TCGGTTAAAG TGGAAAATCA TTTGCTGGGC
    CTGGGGAAGA AGTCATTCCA GAGGTCTGAC AGGCTCCACA CAAGGCAAAT GAAGAGAACT
    AAATGTGCTG AGATTGATGT TGAGACGCCA GATTCCATTC TGGTTAATAC AAACCTACGA
    GCGCTAATCA ACAAACACAC CTTTTCAGTC CTTCCTGGAG ACTGCCAACA GCGATTGCTT
    TTACTACTTC CAGAGGTGGA TCGACAGGTT GGTCCAGATG GTCTGATGAA GTTAAATGGC
    TCAGCCCTTA ATAATGAGTT CTTCACTTCA GCAGCCCAAG GCTGGAAGGA AAGACTTTCA
    GAAGGTGAGT TTACACCGGA GATGCAGGTG AGAATTCGAC AAGAGATTGA GAAGGAGAAG
    AAAGTAGAGC CGTGGAAAGA ACAATTCTTT GAAAGCTACT ATGGTCAGAG TTCTGGCCTG
    AGTCTTGAAG ATTCAAAGAA ATTGACAGCT TCACCCAATG ATCCCAAAGT AAAGAAAACC
    CCAGCTGAGC AACCAAAATC TACGCTTCCT TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA
    GTAATTCCCC AGTTAGAATC TAAAGAGGAA GTACTGCAGA TGCCATCACC AGTCAGAAAA
    GAAGAGCATG AAAGCCAAGA TAAGACGCAG CCACACTCCA CAGAGCCCCT TCTTTCCCCA
    GCTACCAATA CAAATGAGCT TAGCAGCATT CCTCCCATCA AGTGCCCGAA GGATGAGGCT
    CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT GAACAGGAAT CTGAGAAAAA GAATCATCTC
    ACTACAGCTT CAAATTATAA TAAAAATGAA AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC
    AAACCCAAGA GTCCTGGGGT AGAAACAACA GTAATGAAGC CCACAGTAGA AGCAAGTCCA
    CAGGAGACAA CTATGAAAGA GCCTCCATCA ACTCTGGCTG ATCACAGCCC AGAAAGCCTC
    AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG GCCCCAACAA GCTGGGAGAA AAGGCCGCGT
    GTCACTGAGC ATCGCCAGCA CCAGCAGCCA TTTCAAGTCT CCCCACAGCC CTTTCTCAGT
    AGAGGGGACA GGTTCCAGGT GCGGAGAGTA CCACCTCTCA AGATCCCGGT CTCCAGAATC
    TCTCCCATGC CGTTTCCTAC ATCGCAGGTC TCTCCCAGGG CTCGTTTTCC AATCTCCATC
    ACTAGTCCTA ACAGAACAGG AGCCAGAACC CTTGCAGACA TCAAAGCAAA AGCCCAGCTT
    GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT
    GGAGGGACCA TTCCAGGACC TGGCCCAGGC GGTGGACAGG GTCCAGGGGA GGGGGGTGAA
    AGGAGAACTG CTAGAGGAGG GGATCCTGAC TCAGACAAAG TCAGTGAGCC CAGCAAGGGC
    CCCGCACTGG AACTGGCAGG AACTGGAAGC AGGGGAGGTA CGAGAGAGCT TTTACCCGCT
    GGGCCAGACA CTCAGCCCCA GTCTGAGACC AGGACCGCAG GCCAGCCACA GCCTCTTAGT
    GGCCCTGGAG CACAACTACA GCAAACCCCC TCAGTGCCTC CCGCATCTGC CGTCGGTGGA
    GCATGTGCAG GTGTCCCCTC CCCAGCCCAC ATTCATGCAC TCCCGCCAGC TTTAGGAAAA
    TTAAGTAATG AAAAACTAAA TCCCCCCAGG GTAACAGGCA CGGTGGCCTC TCTCAGCCAA
    CCCCAAGGGC CCAGTAATGG TAGGCAGGAG AAAGCACCTC CAGCTCCAGC AGATCCTGCT
    CTGATCATGG GTGCCTCGCC TGTTCATTTT GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA
    GGTTCTAGTA AGAATGCCCC CAATCCTCCG GCCTCAGCAG AGATAAGTGC TAGCGCCTCA
    GTGGATACAA CTGCCTCCCC TTTAACATCT CTGTTAACAA CAGCCACTTT AGAGAAGCTC
    CCTGTGCCGC AGGTCAGCGT AACCACAACG CCTACTGGAT CAGCTCCATC CTTGAGCACT
    TTGCCGGCAG CCTCTAGCCT TAAAACTCCG GGAACTTCTT CACATATGAA TGGACCCATT
    TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT AATCCTTTGG TAACTCAGCT GCTGCAGGGC
    AAAGATGTTC CCATGGAGCA AATTCTGCCT AAACCTCTTA CCAAAGTTGA AATGAAAACT
    GTTCCACTCA CTACAAAAGA GGAGAAGGGA GTAGGAGCAC TCATAGGTAC CAGCGTGACA
    GAGAACAGCA CCAGAGAGGA AGTTCATGAG AGACAGTCCC ATCCAGCTGC ACAGCACCTG
    GGTAAAACTT TGCAAAGTAA GCAGCTCCCC CAGGTTCCAA GACCCCTGCA GCTCTTTTCA
    GGTAAGGATC TAAGGGACTC TGGCATTGAC ACACACCAGT ACCAAGAAGG ACTCAGTAAG
    GCCACCCAAG ATCAGATTCT TCAGACTCTC AGCCAGAGGG TTCGGAGGCA GAACATTCTC
    TCATTTGTGC AGCCCTCACA GTTCAACTTT ACTCACTCAG GTTTCCCGTT AGAGGACATT
    TCCACAAGCC AGAGGTTCAT GCTAGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCGATG
    GCAGGTCACT ACTTACTTAA TATTTCCACC TATGGCCGGG GTACAGAGAG CTTTAGGAGG
    ACCCATTCTG TGAACCCCGA AGACCGATTT TGTCTGAGTA GCCCCACTGA GGCCTTGAAA
    ATGGGATACA CAGACTGTAA AAATGCAGCA GGAGATAGTA GCAGCGGGAA GGAAGATGAT
    ACTGACGAGG AAAGTACTGG TGATGAGCAA GAATCTGTCC CGGTAAAGGA GGAGCCACAG
    GCTTCTCAGA GTTCTGGCAA GTGTGATGCA AGTTTAGGAC CCCACAGTAG AGAAACCCTG
    TCCACCAGTG ACTGTTCCGC TAAAAAGAAT GTGAAGGCAG AGACACCAGT GCCTGAGCAA
    ACCCCTTTAA GTAAGGAGAA TTACCTGTTC ACTCGAGGCC AAACCTTTGA CGAGAAGACC
    CTAGCCAGAG ATTTTATTCA GGCAGCACAG AAGCAGATGG CTCATGCAGT GAGAGGTAAG
    ACCATGCGGA GCAGCCCAGA GCTTTTCAAT TCTACGTCTC TTCCTCTGCC TGCAGACAGT
    CCTACCCATC AATCTCTCCT CCTCCCACCA CTGCAAACCC CAAAGCTGTA CGGGAGCCCC
    ACACAGATCG GGCCAAGCTA CAGAGGCATG ATCAACGTCT CCACCTCATC AGACATGGAC
    CATAACTCCT CTGTCCCGGG GATGCCCGAC TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT
    GTCATGTCCT TTTCAGTGAC TGTCACAGCC ATCCCTGCCA GCCAGGCCGT GAATGCCGGC
    AGCCGCGGCC AGACCATTCC TGTTCAGGCC TTCCCTGAGG AGAGCAGCGT AGAGGACACT
    CCTTCCAAGT GTTACTGCCG GTTGAAAGCC ATGATCATGT GCAAGGGCTG CGGAGCCTTC
    TGCCACGACG ATTGCATTGG CCCCTCCAAA CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06979
    Clone ID Related Accession (Same CDS sequence) XM_010817016.2 , XM_010810275.3 , XM_010810275.2 , XM_024999489.1
    Accession Version XM_010810275.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810275.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCATCT CCTCCAACCA GCACCTCTCT CTGAAGACTG TCAAAGCATC CAGTGACCCT 
    GTACCTGCCA AACCTGCGAT ATGGGAAGGA AAGCAATCTG ATGGACAGTC AAGCAGCCCT
    CAGAACTCAA ACTCTAGCTT TTCTTCTTCT TCGGTTAAAG TGGAAAATCA TTTGCTGGGC
    CTGGGGAAGA AGTCATTCCA GAGGTCTGAC AGGCTCCACA CAAGGCAAAT GAAGAGAACT
    AAATGTGCTG AGATTGATGT TGAGACGCCA GATTCCATTC TGGTTAATAC AAACCTACGA
    GCGCTAATCA ACAAACACAC CTTTTCAGTC CTTCCTGGAG ACTGCCAACA GCGATTGCTT
    TTACTACTTC CAGAGGTGGA TCGACAGGTT GGTCCAGATG GTCTGATGAA GTTAAATGGC
    TCAGCCCTTA ATAATGAGTT CTTCACTTCA GCAGCCCAAG GCTGGAAGGA AAGACTTTCA
    GAAGGTGAGT TTACACCGGA GATGCAGGTG AGAATTCGAC AAGAGATTGA GAAGGAGAAG
    AAAGTAGAGC CGTGGAAAGA ACAATTCTTT GAAAGCTACT ATGGTCAGAG TTCTGGCCTG
    AGTCTTGAAG ATTCAAAGAA ATTGACAGCT TCACCCAATG ATCCCAAAGT AAAGAAAACC
    CCAGCTGAGC AACCAAAATC TACGCTTCCT TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA
    GTAATTCCCC AGTTAGAATC TAAAGAGGAA GTACTGCAGA TGCCATCACC AGTCAGAAAA
    GAAGAGCATG AAAGCCAAGA TAAGACGCAG CCACACTCCA CAGAGCCCCT TCTTTCCCCA
    GCTACCAATA CAAATGAGCT TAGCAGCATT CCTCCCATCA AGTGCCCGAA GGATGAGGCT
    CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT GAACAGGAAT CTGAGAAAAA GAATCATCTC
    ACTACAGCTT CAAATTATAA TAAAAATGAA AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC
    AAACCCAAGA GTCCTGGGGT AGAAACAACA GTAATGAAGC CCACAGTAGA AGCAAGTCCA
    CAGGAGACAA CTATGAAAGA GCCTCCATCA ACTCTGGCTG ATCACAGCCC AGAAAGCCTC
    AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG GCCCCAACAA GCTGGGAGAA AAGGCCGCGT
    GTCACTGAGC ATCGCCAGCA CCAGCAGCCA TTTCAAGTCT CCCCACAGCC CTTTCTCAGT
    AGAGGGGACA GGTTCCAGGT GCGGAGAGTA CCACCTCTCA AGATCCCGGT CTCCAGAATC
    TCTCCCATGC CGTTTCCTAC ATCGCAGGTC TCTCCCAGGG CTCGTTTTCC AATCTCCATC
    ACTAGTCCTA ACAGAACAGG AGCCAGAACC CTTGCAGACA TCAAAGCAAA AGCCCAGCTT
    GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT
    GGAGGGACCA TTCCAGGACC TGGCCCAGGC GGTGGACAGG GTCCAGGGGA GGGGGGTGAA
    AGGAGAACTG CTAGAGGAGG GGATCCTGAC TCAGACAAAG TCAGTGAGCC CAGCAAGGGC
    CCCGCACTGG AACTGGCAGG AACTGGAAGC AGGGGAGGTA CGAGAGAGCT TTTACCCGCT
    GGGCCAGACA CTCAGCCCCA GTCTGAGACC AGGACCGCAG GCCAGCCACA GCCTCTTAGT
    GGCCCTGGAG CACAACTACA GCAAACCCCC TCAGTGCCTC CCGCATCTGC CGTCGGTGGA
    GCATGTGCAG GTGTCCCCTC CCCAGCCCAC ATTCATGCAC TCCCGCCAGC TTTAGGAAAA
    TTAAGTAATG AAAAACTAAA TCCCCCCAGG GTAACAGGCA CGGTGGCCTC TCTCAGCCAA
    CCCCAAGGGC CCAGTAATGG TAGGCAGGAG AAAGCACCTC CAGCTCCAGC AGATCCTGCT
    CTGATCATGG GTGCCTCGCC TGTTCATTTT GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA
    GGTTCTAGTA AGAATGCCCC CAATCCTCCG GCCTCAGCAG AGATAAGTGC TAGCGCCTCA
    GTGGATACAA CTGCCTCCCC TTTAACATCT CTGTTAACAA CAGCCACTTT AGAGAAGCTC
    CCTGTGCCGC AGGTCAGCGT AACCACAACG CCTACTGGAT CAGCTCCATC CTTGAGCACT
    TTGCCGGCAG CCTCTAGCCT TAAAACTCCG GGAACTTCTT CACATATGAA TGGACCCATT
    TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT AATCCTTTGG TAACTCAGCT GCTGCAGGGC
    AAAGATGTTC CCATGGAGCA AATTCTGCCT AAACCTCTTA CCAAAGTTGA AATGAAAACT
    GTTCCACTCA CTACAAAAGA GGAGAAGGGA GTAGGAGCAC TCATAGGTAC CAGCGTGACA
    GAGAACAGCA CCAGAGAGGA AGTTCATGAG AGACAGTCCC ATCCAGCTGC ACAGCACCTG
    GGTAAAACTT TGCAAAGTAA GCAGCTCCCC CAGGTTCCAA GACCCCTGCA GCTCTTTTCA
    GGTAAGGATC TAAGGGACTC TGGCATTGAC ACACACCAGT ACCAAGAAGG ACTCAGTAAG
    GCCACCCAAG ATCAGATTCT TCAGACTCTC AGCCAGAGGG TTCGGAGGCA GAACATTCTC
    TCATTTGTGC AGCCCTCACA GTTCAACTTT ACTCACTCAG GTTTCCCGTT AGAGGACATT
    TCCACAAGCC AGAGGTTCAT GCTAGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCGATG
    GCAGGTCACT ACTTACTTAA TATTTCCACC TATGGCCGGG GTACAGAGAG CTTTAGGAGG
    ACCCATTCTG TGAACCCCGA AGACCGATTT TGTCTGAGTA GCCCCACTGA GGCCTTGAAA
    ATGGGATACA CAGACTGTAA AAATGCAGCA GGAGATAGTA GCAGCGGGAA GGAAGATGAT
    ACTGACGAGG AAAGTACTGG TGATGAGCAA GAATCTGTCC CGGTAAAGGA GGAGCCACAG
    GCTTCTCAGA GTTCTGGCAA GTGTGATGCA AGTTTAGGAC CCCACAGTAG AGAAACCCTG
    TCCACCAGTG ACTGTTCCGC TAAAAAGAAT GTGAAGGCAG AGACACCAGT GCCTGAGCAA
    ACCCCTTTAA GTAAGGAGAA TTACCTGTTC ACTCGAGGCC AAACCTTTGA CGAGAAGACC
    CTAGCCAGAG ATTTTATTCA GGCAGCACAG AAGCAGATGG CTCATGCAGT GAGAGGTAAG
    ACCATGCGGA GCAGCCCAGA GCTTTTCAAT TCTACGTCTC TTCCTCTGCC TGCAGACAGT
    CCTACCCATC AATCTCTCCT CCTCCCACCA CTGCAAACCC CAAAGCTGTA CGGGAGCCCC
    ACACAGATCG GGCCAAGCTA CAGAGGCATG ATCAACGTCT CCACCTCATC AGACATGGAC
    CATAACTCCT CTGTCCCGGG GATGCCCGAC TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT
    GTCATGTCCT TTTCAGTGAC TGTCACAGCC ATCCCTGCCA GCCAGGCCGT GAATGCCGGC
    AGCCGCGGCC AGACCATTCC TGTTCAGGCC TTCCCTGAGG AGAGCAGCGT AGAGGACACT
    CCTTCCAAGT GTTACTGCCG GTTGAAAGCC ATGATCATGT GCAAGGGCTG CGGAGCCTTC
    TGCCACGACG ATTGCATTGG CCCCTCCAAA CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808577.1
    CDS280..4059
    Translation

    Target ORF information:

    RefSeq Version XM_010810275.3
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810275.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCATCT CCTCCAACCA GCACCTCTCT CTGAAGACTG TCAAAGCATC CAGTGACCCT 
    GTACCTGCCA AACCTGCGAT ATGGGAAGGA AAGCAATCTG ATGGACAGTC AAGCAGCCCT
    CAGAACTCAA ACTCTAGCTT TTCTTCTTCT TCGGTTAAAG TGGAAAATCA TTTGCTGGGC
    CTGGGGAAGA AGTCATTCCA GAGGTCTGAC AGGCTCCACA CAAGGCAAAT GAAGAGAACT
    AAATGTGCTG AGATTGATGT TGAGACGCCA GATTCCATTC TGGTTAATAC AAACCTACGA
    GCGCTAATCA ACAAACACAC CTTTTCAGTC CTTCCTGGAG ACTGCCAACA GCGATTGCTT
    TTACTACTTC CAGAGGTGGA TCGACAGGTT GGTCCAGATG GTCTGATGAA GTTAAATGGC
    TCAGCCCTTA ATAATGAGTT CTTCACTTCA GCAGCCCAAG GCTGGAAGGA AAGACTTTCA
    GAAGGTGAGT TTACACCGGA GATGCAGGTG AGAATTCGAC AAGAGATTGA GAAGGAGAAG
    AAAGTAGAGC CGTGGAAAGA ACAATTCTTT GAAAGCTACT ATGGTCAGAG TTCTGGCCTG
    AGTCTTGAAG ATTCAAAGAA ATTGACAGCT TCACCCAATG ATCCCAAAGT AAAGAAAACC
    CCAGCTGAGC AACCAAAATC TACGCTTCCT TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA
    GTAATTCCCC AGTTAGAATC TAAAGAGGAA GTACTGCAGA TGCCATCACC AGTCAGAAAA
    GAAGAGCATG AAAGCCAAGA TAAGACGCAG CCACACTCCA CAGAGCCCCT TCTTTCCCCA
    GCTACCAATA CAAATGAGCT TAGCAGCATT CCTCCCATCA AGTGCCCGAA GGATGAGGCT
    CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT GAACAGGAAT CTGAGAAAAA GAATCATCTC
    ACTACAGCTT CAAATTATAA TAAAAATGAA AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC
    AAACCCAAGA GTCCTGGGGT AGAAACAACA GTAATGAAGC CCACAGTAGA AGCAAGTCCA
    CAGGAGACAA CTATGAAAGA GCCTCCATCA ACTCTGGCTG ATCACAGCCC AGAAAGCCTC
    AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG GCCCCAACAA GCTGGGAGAA AAGGCCGCGT
    GTCACTGAGC ATCGCCAGCA CCAGCAGCCA TTTCAAGTCT CCCCACAGCC CTTTCTCAGT
    AGAGGGGACA GGTTCCAGGT GCGGAGAGTA CCACCTCTCA AGATCCCGGT CTCCAGAATC
    TCTCCCATGC CGTTTCCTAC ATCGCAGGTC TCTCCCAGGG CTCGTTTTCC AATCTCCATC
    ACTAGTCCTA ACAGAACAGG AGCCAGAACC CTTGCAGACA TCAAAGCAAA AGCCCAGCTT
    GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT
    GGAGGGACCA TTCCAGGACC TGGCCCAGGC GGTGGACAGG GTCCAGGGGA GGGGGGTGAA
    AGGAGAACTG CTAGAGGAGG GGATCCTGAC TCAGACAAAG TCAGTGAGCC CAGCAAGGGC
    CCCGCACTGG AACTGGCAGG AACTGGAAGC AGGGGAGGTA CGAGAGAGCT TTTACCCGCT
    GGGCCAGACA CTCAGCCCCA GTCTGAGACC AGGACCGCAG GCCAGCCACA GCCTCTTAGT
    GGCCCTGGAG CACAACTACA GCAAACCCCC TCAGTGCCTC CCGCATCTGC CGTCGGTGGA
    GCATGTGCAG GTGTCCCCTC CCCAGCCCAC ATTCATGCAC TCCCGCCAGC TTTAGGAAAA
    TTAAGTAATG AAAAACTAAA TCCCCCCAGG GTAACAGGCA CGGTGGCCTC TCTCAGCCAA
    CCCCAAGGGC CCAGTAATGG TAGGCAGGAG AAAGCACCTC CAGCTCCAGC AGATCCTGCT
    CTGATCATGG GTGCCTCGCC TGTTCATTTT GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA
    GGTTCTAGTA AGAATGCCCC CAATCCTCCG GCCTCAGCAG AGATAAGTGC TAGCGCCTCA
    GTGGATACAA CTGCCTCCCC TTTAACATCT CTGTTAACAA CAGCCACTTT AGAGAAGCTC
    CCTGTGCCGC AGGTCAGCGT AACCACAACG CCTACTGGAT CAGCTCCATC CTTGAGCACT
    TTGCCGGCAG CCTCTAGCCT TAAAACTCCG GGAACTTCTT CACATATGAA TGGACCCATT
    TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT AATCCTTTGG TAACTCAGCT GCTGCAGGGC
    AAAGATGTTC CCATGGAGCA AATTCTGCCT AAACCTCTTA CCAAAGTTGA AATGAAAACT
    GTTCCACTCA CTACAAAAGA GGAGAAGGGA GTAGGAGCAC TCATAGGTAC CAGCGTGACA
    GAGAACAGCA CCAGAGAGGA AGTTCATGAG AGACAGTCCC ATCCAGCTGC ACAGCACCTG
    GGTAAAACTT TGCAAAGTAA GCAGCTCCCC CAGGTTCCAA GACCCCTGCA GCTCTTTTCA
    GGTAAGGATC TAAGGGACTC TGGCATTGAC ACACACCAGT ACCAAGAAGG ACTCAGTAAG
    GCCACCCAAG ATCAGATTCT TCAGACTCTC AGCCAGAGGG TTCGGAGGCA GAACATTCTC
    TCATTTGTGC AGCCCTCACA GTTCAACTTT ACTCACTCAG GTTTCCCGTT AGAGGACATT
    TCCACAAGCC AGAGGTTCAT GCTAGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCGATG
    GCAGGTCACT ACTTACTTAA TATTTCCACC TATGGCCGGG GTACAGAGAG CTTTAGGAGG
    ACCCATTCTG TGAACCCCGA AGACCGATTT TGTCTGAGTA GCCCCACTGA GGCCTTGAAA
    ATGGGATACA CAGACTGTAA AAATGCAGCA GGAGATAGTA GCAGCGGGAA GGAAGATGAT
    ACTGACGAGG AAAGTACTGG TGATGAGCAA GAATCTGTCC CGGTAAAGGA GGAGCCACAG
    GCTTCTCAGA GTTCTGGCAA GTGTGATGCA AGTTTAGGAC CCCACAGTAG AGAAACCCTG
    TCCACCAGTG ACTGTTCCGC TAAAAAGAAT GTGAAGGCAG AGACACCAGT GCCTGAGCAA
    ACCCCTTTAA GTAAGGAGAA TTACCTGTTC ACTCGAGGCC AAACCTTTGA CGAGAAGACC
    CTAGCCAGAG ATTTTATTCA GGCAGCACAG AAGCAGATGG CTCATGCAGT GAGAGGTAAG
    ACCATGCGGA GCAGCCCAGA GCTTTTCAAT TCTACGTCTC TTCCTCTGCC TGCAGACAGT
    CCTACCCATC AATCTCTCCT CCTCCCACCA CTGCAAACCC CAAAGCTGTA CGGGAGCCCC
    ACACAGATCG GGCCAAGCTA CAGAGGCATG ATCAACGTCT CCACCTCATC AGACATGGAC
    CATAACTCCT CTGTCCCGGG GATGCCCGAC TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT
    GTCATGTCCT TTTCAGTGAC TGTCACAGCC ATCCCTGCCA GCCAGGCCGT GAATGCCGGC
    AGCCGCGGCC AGACCATTCC TGTTCAGGCC TTCCCTGAGG AGAGCAGCGT AGAGGACACT
    CCTTCCAAGT GTTACTGCCG GTTGAAAGCC ATGATCATGT GCAAGGGCTG CGGAGCCTTC
    TGCCACGACG ATTGCATTGG CCCCTCCAAA CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06979
    Clone ID Related Accession (Same CDS sequence) XM_010817016.2 , XM_010810275.3 , XM_010810275.2 , XM_024999489.1
    Accession Version XM_010810275.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCATCT CCTCCAACCA GCACCTCTCT CTGAAGACTG TCAAAGCATC CAGTGACCCT 
    GTACCTGCCA AACCTGCGAT ATGGGAAGGA AAGCAATCTG ATGGACAGTC AAGCAGCCCT
    CAGAACTCAA ACTCTAGCTT TTCTTCTTCT TCGGTTAAAG TGGAAAATCA TTTGCTGGGC
    CTGGGGAAGA AGTCATTCCA GAGGTCTGAC AGGCTCCACA CAAGGCAAAT GAAGAGAACT
    AAATGTGCTG AGATTGATGT TGAGACGCCA GATTCCATTC TGGTTAATAC AAACCTACGA
    GCGCTAATCA ACAAACACAC CTTTTCAGTC CTTCCTGGAG ACTGCCAACA GCGATTGCTT
    TTACTACTTC CAGAGGTGGA TCGACAGGTT GGTCCAGATG GTCTGATGAA GTTAAATGGC
    TCAGCCCTTA ATAATGAGTT CTTCACTTCA GCAGCCCAAG GCTGGAAGGA AAGACTTTCA
    GAAGGTGAGT TTACACCGGA GATGCAGGTG AGAATTCGAC AAGAGATTGA GAAGGAGAAG
    AAAGTAGAGC CGTGGAAAGA ACAATTCTTT GAAAGCTACT ATGGTCAGAG TTCTGGCCTG
    AGTCTTGAAG ATTCAAAGAA ATTGACAGCT TCACCCAATG ATCCCAAAGT AAAGAAAACC
    CCAGCTGAGC AACCAAAATC TACGCTTCCT TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA
    GTAATTCCCC AGTTAGAATC TAAAGAGGAA GTACTGCAGA TGCCATCACC AGTCAGAAAA
    GAAGAGCATG AAAGCCAAGA TAAGACGCAG CCACACTCCA CAGAGCCCCT TCTTTCCCCA
    GCTACCAATA CAAATGAGCT TAGCAGCATT CCTCCCATCA AGTGCCCGAA GGATGAGGCT
    CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT GAACAGGAAT CTGAGAAAAA GAATCATCTC
    ACTACAGCTT CAAATTATAA TAAAAATGAA AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC
    AAACCCAAGA GTCCTGGGGT AGAAACAACA GTAATGAAGC CCACAGTAGA AGCAAGTCCA
    CAGGAGACAA CTATGAAAGA GCCTCCATCA ACTCTGGCTG ATCACAGCCC AGAAAGCCTC
    AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG GCCCCAACAA GCTGGGAGAA AAGGCCGCGT
    GTCACTGAGC ATCGCCAGCA CCAGCAGCCA TTTCAAGTCT CCCCACAGCC CTTTCTCAGT
    AGAGGGGACA GGTTCCAGGT GCGGAGAGTA CCACCTCTCA AGATCCCGGT CTCCAGAATC
    TCTCCCATGC CGTTTCCTAC ATCGCAGGTC TCTCCCAGGG CTCGTTTTCC AATCTCCATC
    ACTAGTCCTA ACAGAACAGG AGCCAGAACC CTTGCAGACA TCAAAGCAAA AGCCCAGCTT
    GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT
    GGAGGGACCA TTCCAGGACC TGGCCCAGGC GGTGGACAGG GTCCAGGGGA GGGGGGTGAA
    AGGAGAACTG CTAGAGGAGG GGATCCTGAC TCAGACAAAG TCAGTGAGCC CAGCAAGGGC
    CCCGCACTGG AACTGGCAGG AACTGGAAGC AGGGGAGGTA CGAGAGAGCT TTTACCCGCT
    GGGCCAGACA CTCAGCCCCA GTCTGAGACC AGGACCGCAG GCCAGCCACA GCCTCTTAGT
    GGCCCTGGAG CACAACTACA GCAAACCCCC TCAGTGCCTC CCGCATCTGC CGTCGGTGGA
    GCATGTGCAG GTGTCCCCTC CCCAGCCCAC ATTCATGCAC TCCCGCCAGC TTTAGGAAAA
    TTAAGTAATG AAAAACTAAA TCCCCCCAGG GTAACAGGCA CGGTGGCCTC TCTCAGCCAA
    CCCCAAGGGC CCAGTAATGG TAGGCAGGAG AAAGCACCTC CAGCTCCAGC AGATCCTGCT
    CTGATCATGG GTGCCTCGCC TGTTCATTTT GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA
    GGTTCTAGTA AGAATGCCCC CAATCCTCCG GCCTCAGCAG AGATAAGTGC TAGCGCCTCA
    GTGGATACAA CTGCCTCCCC TTTAACATCT CTGTTAACAA CAGCCACTTT AGAGAAGCTC
    CCTGTGCCGC AGGTCAGCGT AACCACAACG CCTACTGGAT CAGCTCCATC CTTGAGCACT
    TTGCCGGCAG CCTCTAGCCT TAAAACTCCG GGAACTTCTT CACATATGAA TGGACCCATT
    TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT AATCCTTTGG TAACTCAGCT GCTGCAGGGC
    AAAGATGTTC CCATGGAGCA AATTCTGCCT AAACCTCTTA CCAAAGTTGA AATGAAAACT
    GTTCCACTCA CTACAAAAGA GGAGAAGGGA GTAGGAGCAC TCATAGGTAC CAGCGTGACA
    GAGAACAGCA CCAGAGAGGA AGTTCATGAG AGACAGTCCC ATCCAGCTGC ACAGCACCTG
    GGTAAAACTT TGCAAAGTAA GCAGCTCCCC CAGGTTCCAA GACCCCTGCA GCTCTTTTCA
    GGTAAGGATC TAAGGGACTC TGGCATTGAC ACACACCAGT ACCAAGAAGG ACTCAGTAAG
    GCCACCCAAG ATCAGATTCT TCAGACTCTC AGCCAGAGGG TTCGGAGGCA GAACATTCTC
    TCATTTGTGC AGCCCTCACA GTTCAACTTT ACTCACTCAG GTTTCCCGTT AGAGGACATT
    TCCACAAGCC AGAGGTTCAT GCTAGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCGATG
    GCAGGTCACT ACTTACTTAA TATTTCCACC TATGGCCGGG GTACAGAGAG CTTTAGGAGG
    ACCCATTCTG TGAACCCCGA AGACCGATTT TGTCTGAGTA GCCCCACTGA GGCCTTGAAA
    ATGGGATACA CAGACTGTAA AAATGCAGCA GGAGATAGTA GCAGCGGGAA GGAAGATGAT
    ACTGACGAGG AAAGTACTGG TGATGAGCAA GAATCTGTCC CGGTAAAGGA GGAGCCACAG
    GCTTCTCAGA GTTCTGGCAA GTGTGATGCA AGTTTAGGAC CCCACAGTAG AGAAACCCTG
    TCCACCAGTG ACTGTTCCGC TAAAAAGAAT GTGAAGGCAG AGACACCAGT GCCTGAGCAA
    ACCCCTTTAA GTAAGGAGAA TTACCTGTTC ACTCGAGGCC AAACCTTTGA CGAGAAGACC
    CTAGCCAGAG ATTTTATTCA GGCAGCACAG AAGCAGATGG CTCATGCAGT GAGAGGTAAG
    ACCATGCGGA GCAGCCCAGA GCTTTTCAAT TCTACGTCTC TTCCTCTGCC TGCAGACAGT
    CCTACCCATC AATCTCTCCT CCTCCCACCA CTGCAAACCC CAAAGCTGTA CGGGAGCCCC
    ACACAGATCG GGCCAAGCTA CAGAGGCATG ATCAACGTCT CCACCTCATC AGACATGGAC
    CATAACTCCT CTGTCCCGGG GATGCCCGAC TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT
    GTCATGTCCT TTTCAGTGAC TGTCACAGCC ATCCCTGCCA GCCAGGCCGT GAATGCCGGC
    AGCCGCGGCC AGACCATTCC TGTTCAGGCC TTCCCTGAGG AGAGCAGCGT AGAGGACACT
    CCTTCCAAGT GTTACTGCCG GTTGAAAGCC ATGATCATGT GCAAGGGCTG CGGAGCCTTC
    TGCCACGACG ATTGCATTGG CCCCTCCAAA CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808577.1
    CDS275..4054
    Translation

    Target ORF information:

    RefSeq Version XM_010810275.2
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810275.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCATCT CCTCCAACCA GCACCTCTCT CTGAAGACTG TCAAAGCATC CAGTGACCCT 
    GTACCTGCCA AACCTGCGAT ATGGGAAGGA AAGCAATCTG ATGGACAGTC AAGCAGCCCT
    CAGAACTCAA ACTCTAGCTT TTCTTCTTCT TCGGTTAAAG TGGAAAATCA TTTGCTGGGC
    CTGGGGAAGA AGTCATTCCA GAGGTCTGAC AGGCTCCACA CAAGGCAAAT GAAGAGAACT
    AAATGTGCTG AGATTGATGT TGAGACGCCA GATTCCATTC TGGTTAATAC AAACCTACGA
    GCGCTAATCA ACAAACACAC CTTTTCAGTC CTTCCTGGAG ACTGCCAACA GCGATTGCTT
    TTACTACTTC CAGAGGTGGA TCGACAGGTT GGTCCAGATG GTCTGATGAA GTTAAATGGC
    TCAGCCCTTA ATAATGAGTT CTTCACTTCA GCAGCCCAAG GCTGGAAGGA AAGACTTTCA
    GAAGGTGAGT TTACACCGGA GATGCAGGTG AGAATTCGAC AAGAGATTGA GAAGGAGAAG
    AAAGTAGAGC CGTGGAAAGA ACAATTCTTT GAAAGCTACT ATGGTCAGAG TTCTGGCCTG
    AGTCTTGAAG ATTCAAAGAA ATTGACAGCT TCACCCAATG ATCCCAAAGT AAAGAAAACC
    CCAGCTGAGC AACCAAAATC TACGCTTCCT TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA
    GTAATTCCCC AGTTAGAATC TAAAGAGGAA GTACTGCAGA TGCCATCACC AGTCAGAAAA
    GAAGAGCATG AAAGCCAAGA TAAGACGCAG CCACACTCCA CAGAGCCCCT TCTTTCCCCA
    GCTACCAATA CAAATGAGCT TAGCAGCATT CCTCCCATCA AGTGCCCGAA GGATGAGGCT
    CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT GAACAGGAAT CTGAGAAAAA GAATCATCTC
    ACTACAGCTT CAAATTATAA TAAAAATGAA AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC
    AAACCCAAGA GTCCTGGGGT AGAAACAACA GTAATGAAGC CCACAGTAGA AGCAAGTCCA
    CAGGAGACAA CTATGAAAGA GCCTCCATCA ACTCTGGCTG ATCACAGCCC AGAAAGCCTC
    AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG GCCCCAACAA GCTGGGAGAA AAGGCCGCGT
    GTCACTGAGC ATCGCCAGCA CCAGCAGCCA TTTCAAGTCT CCCCACAGCC CTTTCTCAGT
    AGAGGGGACA GGTTCCAGGT GCGGAGAGTA CCACCTCTCA AGATCCCGGT CTCCAGAATC
    TCTCCCATGC CGTTTCCTAC ATCGCAGGTC TCTCCCAGGG CTCGTTTTCC AATCTCCATC
    ACTAGTCCTA ACAGAACAGG AGCCAGAACC CTTGCAGACA TCAAAGCAAA AGCCCAGCTT
    GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT
    GGAGGGACCA TTCCAGGACC TGGCCCAGGC GGTGGACAGG GTCCAGGGGA GGGGGGTGAA
    AGGAGAACTG CTAGAGGAGG GGATCCTGAC TCAGACAAAG TCAGTGAGCC CAGCAAGGGC
    CCCGCACTGG AACTGGCAGG AACTGGAAGC AGGGGAGGTA CGAGAGAGCT TTTACCCGCT
    GGGCCAGACA CTCAGCCCCA GTCTGAGACC AGGACCGCAG GCCAGCCACA GCCTCTTAGT
    GGCCCTGGAG CACAACTACA GCAAACCCCC TCAGTGCCTC CCGCATCTGC CGTCGGTGGA
    GCATGTGCAG GTGTCCCCTC CCCAGCCCAC ATTCATGCAC TCCCGCCAGC TTTAGGAAAA
    TTAAGTAATG AAAAACTAAA TCCCCCCAGG GTAACAGGCA CGGTGGCCTC TCTCAGCCAA
    CCCCAAGGGC CCAGTAATGG TAGGCAGGAG AAAGCACCTC CAGCTCCAGC AGATCCTGCT
    CTGATCATGG GTGCCTCGCC TGTTCATTTT GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA
    GGTTCTAGTA AGAATGCCCC CAATCCTCCG GCCTCAGCAG AGATAAGTGC TAGCGCCTCA
    GTGGATACAA CTGCCTCCCC TTTAACATCT CTGTTAACAA CAGCCACTTT AGAGAAGCTC
    CCTGTGCCGC AGGTCAGCGT AACCACAACG CCTACTGGAT CAGCTCCATC CTTGAGCACT
    TTGCCGGCAG CCTCTAGCCT TAAAACTCCG GGAACTTCTT CACATATGAA TGGACCCATT
    TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT AATCCTTTGG TAACTCAGCT GCTGCAGGGC
    AAAGATGTTC CCATGGAGCA AATTCTGCCT AAACCTCTTA CCAAAGTTGA AATGAAAACT
    GTTCCACTCA CTACAAAAGA GGAGAAGGGA GTAGGAGCAC TCATAGGTAC CAGCGTGACA
    GAGAACAGCA CCAGAGAGGA AGTTCATGAG AGACAGTCCC ATCCAGCTGC ACAGCACCTG
    GGTAAAACTT TGCAAAGTAA GCAGCTCCCC CAGGTTCCAA GACCCCTGCA GCTCTTTTCA
    GGTAAGGATC TAAGGGACTC TGGCATTGAC ACACACCAGT ACCAAGAAGG ACTCAGTAAG
    GCCACCCAAG ATCAGATTCT TCAGACTCTC AGCCAGAGGG TTCGGAGGCA GAACATTCTC
    TCATTTGTGC AGCCCTCACA GTTCAACTTT ACTCACTCAG GTTTCCCGTT AGAGGACATT
    TCCACAAGCC AGAGGTTCAT GCTAGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCGATG
    GCAGGTCACT ACTTACTTAA TATTTCCACC TATGGCCGGG GTACAGAGAG CTTTAGGAGG
    ACCCATTCTG TGAACCCCGA AGACCGATTT TGTCTGAGTA GCCCCACTGA GGCCTTGAAA
    ATGGGATACA CAGACTGTAA AAATGCAGCA GGAGATAGTA GCAGCGGGAA GGAAGATGAT
    ACTGACGAGG AAAGTACTGG TGATGAGCAA GAATCTGTCC CGGTAAAGGA GGAGCCACAG
    GCTTCTCAGA GTTCTGGCAA GTGTGATGCA AGTTTAGGAC CCCACAGTAG AGAAACCCTG
    TCCACCAGTG ACTGTTCCGC TAAAAAGAAT GTGAAGGCAG AGACACCAGT GCCTGAGCAA
    ACCCCTTTAA GTAAGGAGAA TTACCTGTTC ACTCGAGGCC AAACCTTTGA CGAGAAGACC
    CTAGCCAGAG ATTTTATTCA GGCAGCACAG AAGCAGATGG CTCATGCAGT GAGAGGTAAG
    ACCATGCGGA GCAGCCCAGA GCTTTTCAAT TCTACGTCTC TTCCTCTGCC TGCAGACAGT
    CCTACCCATC AATCTCTCCT CCTCCCACCA CTGCAAACCC CAAAGCTGTA CGGGAGCCCC
    ACACAGATCG GGCCAAGCTA CAGAGGCATG ATCAACGTCT CCACCTCATC AGACATGGAC
    CATAACTCCT CTGTCCCGGG GATGCCCGAC TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT
    GTCATGTCCT TTTCAGTGAC TGTCACAGCC ATCCCTGCCA GCCAGGCCGT GAATGCCGGC
    AGCCGCGGCC AGACCATTCC TGTTCAGGCC TTCCCTGAGG AGAGCAGCGT AGAGGACACT
    CCTTCCAAGT GTTACTGCCG GTTGAAAGCC ATGATCATGT GCAAGGGCTG CGGAGCCTTC
    TGCCACGACG ATTGCATTGG CCCCTCCAAA CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06979
    Clone ID Related Accession (Same CDS sequence) XM_010817016.2 , XM_010810275.3 , XM_010810275.2 , XM_024999489.1
    Accession Version XM_024999489.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCATCT CCTCCAACCA GCACCTCTCT CTGAAGACTG TCAAAGCATC CAGTGACCCT 
    GTACCTGCCA AACCTGCGAT ATGGGAAGGA AAGCAATCTG ATGGACAGTC AAGCAGCCCT
    CAGAACTCAA ACTCTAGCTT TTCTTCTTCT TCGGTTAAAG TGGAAAATCA TTTGCTGGGC
    CTGGGGAAGA AGTCATTCCA GAGGTCTGAC AGGCTCCACA CAAGGCAAAT GAAGAGAACT
    AAATGTGCTG AGATTGATGT TGAGACGCCA GATTCCATTC TGGTTAATAC AAACCTACGA
    GCGCTAATCA ACAAACACAC CTTTTCAGTC CTTCCTGGAG ACTGCCAACA GCGATTGCTT
    TTACTACTTC CAGAGGTGGA TCGACAGGTT GGTCCAGATG GTCTGATGAA GTTAAATGGC
    TCAGCCCTTA ATAATGAGTT CTTCACTTCA GCAGCCCAAG GCTGGAAGGA AAGACTTTCA
    GAAGGTGAGT TTACACCGGA GATGCAGGTG AGAATTCGAC AAGAGATTGA GAAGGAGAAG
    AAAGTAGAGC CGTGGAAAGA ACAATTCTTT GAAAGCTACT ATGGTCAGAG TTCTGGCCTG
    AGTCTTGAAG ATTCAAAGAA ATTGACAGCT TCACCCAATG ATCCCAAAGT AAAGAAAACC
    CCAGCTGAGC AACCAAAATC TACGCTTCCT TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA
    GTAATTCCCC AGTTAGAATC TAAAGAGGAA GTACTGCAGA TGCCATCACC AGTCAGAAAA
    GAAGAGCATG AAAGCCAAGA TAAGACGCAG CCACACTCCA CAGAGCCCCT TCTTTCCCCA
    GCTACCAATA CAAATGAGCT TAGCAGCATT CCTCCCATCA AGTGCCCGAA GGATGAGGCT
    CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT GAACAGGAAT CTGAGAAAAA GAATCATCTC
    ACTACAGCTT CAAATTATAA TAAAAATGAA AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC
    AAACCCAAGA GTCCTGGGGT AGAAACAACA GTAATGAAGC CCACAGTAGA AGCAAGTCCA
    CAGGAGACAA CTATGAAAGA GCCTCCATCA ACTCTGGCTG ATCACAGCCC AGAAAGCCTC
    AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG GCCCCAACAA GCTGGGAGAA AAGGCCGCGT
    GTCACTGAGC ATCGCCAGCA CCAGCAGCCA TTTCAAGTCT CCCCACAGCC CTTTCTCAGT
    AGAGGGGACA GGTTCCAGGT GCGGAGAGTA CCACCTCTCA AGATCCCGGT CTCCAGAATC
    TCTCCCATGC CGTTTCCTAC ATCGCAGGTC TCTCCCAGGG CTCGTTTTCC AATCTCCATC
    ACTAGTCCTA ACAGAACAGG AGCCAGAACC CTTGCAGACA TCAAAGCAAA AGCCCAGCTT
    GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT
    GGAGGGACCA TTCCAGGACC TGGCCCAGGC GGTGGACAGG GTCCAGGGGA GGGGGGTGAA
    AGGAGAACTG CTAGAGGAGG GGATCCTGAC TCAGACAAAG TCAGTGAGCC CAGCAAGGGC
    CCCGCACTGG AACTGGCAGG AACTGGAAGC AGGGGAGGTA CGAGAGAGCT TTTACCCGCT
    GGGCCAGACA CTCAGCCCCA GTCTGAGACC AGGACCGCAG GCCAGCCACA GCCTCTTAGT
    GGCCCTGGAG CACAACTACA GCAAACCCCC TCAGTGCCTC CCGCATCTGC CGTCGGTGGA
    GCATGTGCAG GTGTCCCCTC CCCAGCCCAC ATTCATGCAC TCCCGCCAGC TTTAGGAAAA
    TTAAGTAATG AAAAACTAAA TCCCCCCAGG GTAACAGGCA CGGTGGCCTC TCTCAGCCAA
    CCCCAAGGGC CCAGTAATGG TAGGCAGGAG AAAGCACCTC CAGCTCCAGC AGATCCTGCT
    CTGATCATGG GTGCCTCGCC TGTTCATTTT GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA
    GGTTCTAGTA AGAATGCCCC CAATCCTCCG GCCTCAGCAG AGATAAGTGC TAGCGCCTCA
    GTGGATACAA CTGCCTCCCC TTTAACATCT CTGTTAACAA CAGCCACTTT AGAGAAGCTC
    CCTGTGCCGC AGGTCAGCGT AACCACAACG CCTACTGGAT CAGCTCCATC CTTGAGCACT
    TTGCCGGCAG CCTCTAGCCT TAAAACTCCG GGAACTTCTT CACATATGAA TGGACCCATT
    TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT AATCCTTTGG TAACTCAGCT GCTGCAGGGC
    AAAGATGTTC CCATGGAGCA AATTCTGCCT AAACCTCTTA CCAAAGTTGA AATGAAAACT
    GTTCCACTCA CTACAAAAGA GGAGAAGGGA GTAGGAGCAC TCATAGGTAC CAGCGTGACA
    GAGAACAGCA CCAGAGAGGA AGTTCATGAG AGACAGTCCC ATCCAGCTGC ACAGCACCTG
    GGTAAAACTT TGCAAAGTAA GCAGCTCCCC CAGGTTCCAA GACCCCTGCA GCTCTTTTCA
    GGTAAGGATC TAAGGGACTC TGGCATTGAC ACACACCAGT ACCAAGAAGG ACTCAGTAAG
    GCCACCCAAG ATCAGATTCT TCAGACTCTC AGCCAGAGGG TTCGGAGGCA GAACATTCTC
    TCATTTGTGC AGCCCTCACA GTTCAACTTT ACTCACTCAG GTTTCCCGTT AGAGGACATT
    TCCACAAGCC AGAGGTTCAT GCTAGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCGATG
    GCAGGTCACT ACTTACTTAA TATTTCCACC TATGGCCGGG GTACAGAGAG CTTTAGGAGG
    ACCCATTCTG TGAACCCCGA AGACCGATTT TGTCTGAGTA GCCCCACTGA GGCCTTGAAA
    ATGGGATACA CAGACTGTAA AAATGCAGCA GGAGATAGTA GCAGCGGGAA GGAAGATGAT
    ACTGACGAGG AAAGTACTGG TGATGAGCAA GAATCTGTCC CGGTAAAGGA GGAGCCACAG
    GCTTCTCAGA GTTCTGGCAA GTGTGATGCA AGTTTAGGAC CCCACAGTAG AGAAACCCTG
    TCCACCAGTG ACTGTTCCGC TAAAAAGAAT GTGAAGGCAG AGACACCAGT GCCTGAGCAA
    ACCCCTTTAA GTAAGGAGAA TTACCTGTTC ACTCGAGGCC AAACCTTTGA CGAGAAGACC
    CTAGCCAGAG ATTTTATTCA GGCAGCACAG AAGCAGATGG CTCATGCAGT GAGAGGTAAG
    ACCATGCGGA GCAGCCCAGA GCTTTTCAAT TCTACGTCTC TTCCTCTGCC TGCAGACAGT
    CCTACCCATC AATCTCTCCT CCTCCCACCA CTGCAAACCC CAAAGCTGTA CGGGAGCCCC
    ACACAGATCG GGCCAAGCTA CAGAGGCATG ATCAACGTCT CCACCTCATC AGACATGGAC
    CATAACTCCT CTGTCCCGGG GATGCCCGAC TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT
    GTCATGTCCT TTTCAGTGAC TGTCACAGCC ATCCCTGCCA GCCAGGCCGT GAATGCCGGC
    AGCCGCGGCC AGACCATTCC TGTTCAGGCC TTCCCTGAGG AGAGCAGCGT AGAGGACACT
    CCTTCCAAGT GTTACTGCCG GTTGAAAGCC ATGATCATGT GCAAGGGCTG CGGAGCCTTC
    TGCCACGACG ATTGCATTGG CCCCTCCAAA CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855257.1
    CDS301..4080
    Translation

    Target ORF information:

    RefSeq Version XM_024999489.1
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCCATCT CCTCCAACCA GCACCTCTCT CTGAAGACTG TCAAAGCATC CAGTGACCCT 
    GTACCTGCCA AACCTGCGAT ATGGGAAGGA AAGCAATCTG ATGGACAGTC AAGCAGCCCT
    CAGAACTCAA ACTCTAGCTT TTCTTCTTCT TCGGTTAAAG TGGAAAATCA TTTGCTGGGC
    CTGGGGAAGA AGTCATTCCA GAGGTCTGAC AGGCTCCACA CAAGGCAAAT GAAGAGAACT
    AAATGTGCTG AGATTGATGT TGAGACGCCA GATTCCATTC TGGTTAATAC AAACCTACGA
    GCGCTAATCA ACAAACACAC CTTTTCAGTC CTTCCTGGAG ACTGCCAACA GCGATTGCTT
    TTACTACTTC CAGAGGTGGA TCGACAGGTT GGTCCAGATG GTCTGATGAA GTTAAATGGC
    TCAGCCCTTA ATAATGAGTT CTTCACTTCA GCAGCCCAAG GCTGGAAGGA AAGACTTTCA
    GAAGGTGAGT TTACACCGGA GATGCAGGTG AGAATTCGAC AAGAGATTGA GAAGGAGAAG
    AAAGTAGAGC CGTGGAAAGA ACAATTCTTT GAAAGCTACT ATGGTCAGAG TTCTGGCCTG
    AGTCTTGAAG ATTCAAAGAA ATTGACAGCT TCACCCAATG ATCCCAAAGT AAAGAAAACC
    CCAGCTGAGC AACCAAAATC TACGCTTCCT TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA
    GTAATTCCCC AGTTAGAATC TAAAGAGGAA GTACTGCAGA TGCCATCACC AGTCAGAAAA
    GAAGAGCATG AAAGCCAAGA TAAGACGCAG CCACACTCCA CAGAGCCCCT TCTTTCCCCA
    GCTACCAATA CAAATGAGCT TAGCAGCATT CCTCCCATCA AGTGCCCGAA GGATGAGGCT
    CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT GAACAGGAAT CTGAGAAAAA GAATCATCTC
    ACTACAGCTT CAAATTATAA TAAAAATGAA AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC
    AAACCCAAGA GTCCTGGGGT AGAAACAACA GTAATGAAGC CCACAGTAGA AGCAAGTCCA
    CAGGAGACAA CTATGAAAGA GCCTCCATCA ACTCTGGCTG ATCACAGCCC AGAAAGCCTC
    AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG GCCCCAACAA GCTGGGAGAA AAGGCCGCGT
    GTCACTGAGC ATCGCCAGCA CCAGCAGCCA TTTCAAGTCT CCCCACAGCC CTTTCTCAGT
    AGAGGGGACA GGTTCCAGGT GCGGAGAGTA CCACCTCTCA AGATCCCGGT CTCCAGAATC
    TCTCCCATGC CGTTTCCTAC ATCGCAGGTC TCTCCCAGGG CTCGTTTTCC AATCTCCATC
    ACTAGTCCTA ACAGAACAGG AGCCAGAACC CTTGCAGACA TCAAAGCAAA AGCCCAGCTT
    GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT
    GGAGGGACCA TTCCAGGACC TGGCCCAGGC GGTGGACAGG GTCCAGGGGA GGGGGGTGAA
    AGGAGAACTG CTAGAGGAGG GGATCCTGAC TCAGACAAAG TCAGTGAGCC CAGCAAGGGC
    CCCGCACTGG AACTGGCAGG AACTGGAAGC AGGGGAGGTA CGAGAGAGCT TTTACCCGCT
    GGGCCAGACA CTCAGCCCCA GTCTGAGACC AGGACCGCAG GCCAGCCACA GCCTCTTAGT
    GGCCCTGGAG CACAACTACA GCAAACCCCC TCAGTGCCTC CCGCATCTGC CGTCGGTGGA
    GCATGTGCAG GTGTCCCCTC CCCAGCCCAC ATTCATGCAC TCCCGCCAGC TTTAGGAAAA
    TTAAGTAATG AAAAACTAAA TCCCCCCAGG GTAACAGGCA CGGTGGCCTC TCTCAGCCAA
    CCCCAAGGGC CCAGTAATGG TAGGCAGGAG AAAGCACCTC CAGCTCCAGC AGATCCTGCT
    CTGATCATGG GTGCCTCGCC TGTTCATTTT GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA
    GGTTCTAGTA AGAATGCCCC CAATCCTCCG GCCTCAGCAG AGATAAGTGC TAGCGCCTCA
    GTGGATACAA CTGCCTCCCC TTTAACATCT CTGTTAACAA CAGCCACTTT AGAGAAGCTC
    CCTGTGCCGC AGGTCAGCGT AACCACAACG CCTACTGGAT CAGCTCCATC CTTGAGCACT
    TTGCCGGCAG CCTCTAGCCT TAAAACTCCG GGAACTTCTT CACATATGAA TGGACCCATT
    TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT AATCCTTTGG TAACTCAGCT GCTGCAGGGC
    AAAGATGTTC CCATGGAGCA AATTCTGCCT AAACCTCTTA CCAAAGTTGA AATGAAAACT
    GTTCCACTCA CTACAAAAGA GGAGAAGGGA GTAGGAGCAC TCATAGGTAC CAGCGTGACA
    GAGAACAGCA CCAGAGAGGA AGTTCATGAG AGACAGTCCC ATCCAGCTGC ACAGCACCTG
    GGTAAAACTT TGCAAAGTAA GCAGCTCCCC CAGGTTCCAA GACCCCTGCA GCTCTTTTCA
    GGTAAGGATC TAAGGGACTC TGGCATTGAC ACACACCAGT ACCAAGAAGG ACTCAGTAAG
    GCCACCCAAG ATCAGATTCT TCAGACTCTC AGCCAGAGGG TTCGGAGGCA GAACATTCTC
    TCATTTGTGC AGCCCTCACA GTTCAACTTT ACTCACTCAG GTTTCCCGTT AGAGGACATT
    TCCACAAGCC AGAGGTTCAT GCTAGGTTTT GCTGGCAGAA GGACATCCAA GCCTGCGATG
    GCAGGTCACT ACTTACTTAA TATTTCCACC TATGGCCGGG GTACAGAGAG CTTTAGGAGG
    ACCCATTCTG TGAACCCCGA AGACCGATTT TGTCTGAGTA GCCCCACTGA GGCCTTGAAA
    ATGGGATACA CAGACTGTAA AAATGCAGCA GGAGATAGTA GCAGCGGGAA GGAAGATGAT
    ACTGACGAGG AAAGTACTGG TGATGAGCAA GAATCTGTCC CGGTAAAGGA GGAGCCACAG
    GCTTCTCAGA GTTCTGGCAA GTGTGATGCA AGTTTAGGAC CCCACAGTAG AGAAACCCTG
    TCCACCAGTG ACTGTTCCGC TAAAAAGAAT GTGAAGGCAG AGACACCAGT GCCTGAGCAA
    ACCCCTTTAA GTAAGGAGAA TTACCTGTTC ACTCGAGGCC AAACCTTTGA CGAGAAGACC
    CTAGCCAGAG ATTTTATTCA GGCAGCACAG AAGCAGATGG CTCATGCAGT GAGAGGTAAG
    ACCATGCGGA GCAGCCCAGA GCTTTTCAAT TCTACGTCTC TTCCTCTGCC TGCAGACAGT
    CCTACCCATC AATCTCTCCT CCTCCCACCA CTGCAAACCC CAAAGCTGTA CGGGAGCCCC
    ACACAGATCG GGCCAAGCTA CAGAGGCATG ATCAACGTCT CCACCTCATC AGACATGGAC
    CATAACTCCT CTGTCCCGGG GATGCCCGAC TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT
    GTCATGTCCT TTTCAGTGAC TGTCACAGCC ATCCCTGCCA GCCAGGCCGT GAATGCCGGC
    AGCCGCGGCC AGACCATTCC TGTTCAGGCC TTCCCTGAGG AGAGCAGCGT AGAGGACACT
    CCTTCCAAGT GTTACTGCCG GTTGAAAGCC ATGATCATGT GCAAGGGCTG CGGAGCCTTC
    TGCCACGACG ATTGCATTGG CCCCTCCAAA CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59105
    Clone ID Related Accession (Same CDS sequence) XM_010810276.2 , XM_010817021.2 , XM_010810276.3
    Accession Version XM_010810276.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGACAGTC CTACCCATCA ATCTCTCCTC
    CTCCCACCAC TGCAAACCCC AAAGCTGTAC GGGAGCCCCA CACAGATCGG GCCAAGCTAC
    AGAGGCATGA TCAACGTCTC CACCTCATCA GACATGGACC ATAACTCCTC TGTCCCGGGG
    ATGCCCGACT GTAGTCAGGT GTCTAGCAAT GTTGGTGATG TCATGTCCTT TTCAGTGACT
    GTCACAGCCA TCCCTGCCAG CCAGGCCGTG AATGCCGGCA GCCGCGGCCA GACCATTCCT
    GTTCAGGCCT TCCCTGAGGA GAGCAGCGTA GAGGACACTC CTTCCAAGTG TTACTGCCGG
    TTGAAAGCCA TGATCATGTG CAAGGGCTGC GGAGCCTTCT GCCACGACGA TTGCATTGGC
    CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT GTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808578.1
    CDS362..3940
    Translation

    Target ORF information:

    RefSeq Version XM_010810276.2
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810276.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGACAGTC CTACCCATCA ATCTCTCCTC
    CTCCCACCAC TGCAAACCCC AAAGCTGTAC GGGAGCCCCA CACAGATCGG GCCAAGCTAC
    AGAGGCATGA TCAACGTCTC CACCTCATCA GACATGGACC ATAACTCCTC TGTCCCGGGG
    ATGCCCGACT GTAGTCAGGT GTCTAGCAAT GTTGGTGATG TCATGTCCTT TTCAGTGACT
    GTCACAGCCA TCCCTGCCAG CCAGGCCGTG AATGCCGGCA GCCGCGGCCA GACCATTCCT
    GTTCAGGCCT TCCCTGAGGA GAGCAGCGTA GAGGACACTC CTTCCAAGTG TTACTGCCGG
    TTGAAAGCCA TGATCATGTG CAAGGGCTGC GGAGCCTTCT GCCACGACGA TTGCATTGGC
    CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT GTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59105
    Clone ID Related Accession (Same CDS sequence) XM_010810276.2 , XM_010817021.2 , XM_010810276.3
    Accession Version XM_010817021.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGACAGTC CTACCCATCA ATCTCTCCTC
    CTCCCACCAC TGCAAACCCC AAAGCTGTAC GGGAGCCCCA CACAGATCGG GCCAAGCTAC
    AGAGGCATGA TCAACGTCTC CACCTCATCA GACATGGACC ATAACTCCTC TGTCCCGGGG
    ATGCCCGACT GTAGTCAGGT GTCTAGCAAT GTTGGTGATG TCATGTCCTT TTCAGTGACT
    GTCACAGCCA TCCCTGCCAG CCAGGCCGTG AATGCCGGCA GCCGCGGCCA GACCATTCCT
    GTTCAGGCCT TCCCTGAGGA GAGCAGCGTA GAGGACACTC CTTCCAAGTG TTACTGCCGG
    TTGAAAGCCA TGATCATGTG CAAGGGCTGC GGAGCCTTCT GCCACGACGA TTGCATTGGC
    CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT GTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815323.2
    CDS362..3940
    Translation

    Target ORF information:

    RefSeq Version XM_010817021.2
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817021.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGACAGTC CTACCCATCA ATCTCTCCTC
    CTCCCACCAC TGCAAACCCC AAAGCTGTAC GGGAGCCCCA CACAGATCGG GCCAAGCTAC
    AGAGGCATGA TCAACGTCTC CACCTCATCA GACATGGACC ATAACTCCTC TGTCCCGGGG
    ATGCCCGACT GTAGTCAGGT GTCTAGCAAT GTTGGTGATG TCATGTCCTT TTCAGTGACT
    GTCACAGCCA TCCCTGCCAG CCAGGCCGTG AATGCCGGCA GCCGCGGCCA GACCATTCCT
    GTTCAGGCCT TCCCTGAGGA GAGCAGCGTA GAGGACACTC CTTCCAAGTG TTACTGCCGG
    TTGAAAGCCA TGATCATGTG CAAGGGCTGC GGAGCCTTCT GCCACGACGA TTGCATTGGC
    CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT GTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa59105
    Clone ID Related Accession (Same CDS sequence) XM_010810276.2 , XM_010817021.2 , XM_010810276.3
    Accession Version XM_010810276.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810276.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGACAGTC CTACCCATCA ATCTCTCCTC
    CTCCCACCAC TGCAAACCCC AAAGCTGTAC GGGAGCCCCA CACAGATCGG GCCAAGCTAC
    AGAGGCATGA TCAACGTCTC CACCTCATCA GACATGGACC ATAACTCCTC TGTCCCGGGG
    ATGCCCGACT GTAGTCAGGT GTCTAGCAAT GTTGGTGATG TCATGTCCTT TTCAGTGACT
    GTCACAGCCA TCCCTGCCAG CCAGGCCGTG AATGCCGGCA GCCGCGGCCA GACCATTCCT
    GTTCAGGCCT TCCCTGAGGA GAGCAGCGTA GAGGACACTC CTTCCAAGTG TTACTGCCGG
    TTGAAAGCCA TGATCATGTG CAAGGGCTGC GGAGCCTTCT GCCACGACGA TTGCATTGGC
    CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT GTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808578.1
    CDS285..3863
    Translation

    Target ORF information:

    RefSeq Version XM_010810276.3
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810276.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGACAGTC CTACCCATCA ATCTCTCCTC
    CTCCCACCAC TGCAAACCCC AAAGCTGTAC GGGAGCCCCA CACAGATCGG GCCAAGCTAC
    AGAGGCATGA TCAACGTCTC CACCTCATCA GACATGGACC ATAACTCCTC TGTCCCGGGG
    ATGCCCGACT GTAGTCAGGT GTCTAGCAAT GTTGGTGATG TCATGTCCTT TTCAGTGACT
    GTCACAGCCA TCCCTGCCAG CCAGGCCGTG AATGCCGGCA GCCGCGGCCA GACCATTCCT
    GTTCAGGCCT TCCCTGAGGA GAGCAGCGTA GAGGACACTC CTTCCAAGTG TTACTGCCGG
    TTGAAAGCCA TGATCATGTG CAAGGGCTGC GGAGCCTTCT GCCACGACGA TTGCATTGGC
    CCCTCCAAAC TCTGTGTCTC CTGCCTTGTT GTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24264
    Clone ID Related Accession (Same CDS sequence) XM_002691461.4 , XM_010816993.2 , XM_002691461.5
    Accession Version XM_002691461.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002691461.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGGTAAGG ATCTAAGGGA CTCTGGCATT
    GACACACACC AGTACCAAGA AGGACTCAGT AAGGCCACCC AAGATCAGAT TCTTCAGACT
    CTCAGCCAGA GGGTTCGGAG GCAGAACATT CTCTCATTTG TGCAGCCCTC ACAGTTCAAC
    TTTACTCACT CAGGTTTCCC GTTAGAGGAC ATTTCCACAA GCCAGAGGTT CATGCTAGGT
    TTTGCTGGCA GAAGGACATC CAAGCCTGCG ATGGCAGGTC ACTACTTACT TAATATTTCC
    ACCTATGGCC GGGGTACAGA GAGCTTTAGG AGGACCCATT CTGTGAACCC CGAAGACCGA
    TTTTGTCTGA GTAGCCCCAC TGAGGCCTTG AAAATGGGAT ACACAGACTG TAAAAATGCA
    GCAGGAGATA GTAGCAGCGG GAAGGAAGAT GATACTGACG AGGAAAGTAC TGGTGATGAG
    CAAGAATCTG TCCCGGTAAA GGAGGAGCCA CAGGCTTCTC AGAGTTCTGG CAAGTGTGAT
    GCAAGTTTAG GACCCCACAG TAGAGAAACC CTGTCCACCA GTGACTGTTC CGCTAAAAAG
    AATGTGAAGG CAGAGACACC AGTGCCTGAG CAAACCCCTT TAAGTAAGGA GAATTACCTG
    TTCACTCGAG GCCAAACCTT TGACGAGAAG ACCCTAGCCA GAGATTTTAT TCAGGCAGCA
    CAGAAGCAGA TGGCTCATGC AGTGAGAGGT AAGACCATGC GGAGCAGCCC AGAGCTTTTC
    AATTCTACGT CTCTTCCTCT GCCTGCAGAC AGTCCTACCC ATCAATCTCT CCTCCTCCCA
    CCACTGCAAA CCCCAAAGCT GTACGGGAGC CCCACACAGA TCGGGCCAAG CTACAGAGGC
    ATGATCAACG TCTCCACCTC ATCAGACATG GACCATAACT CCTCTGTCCC GGGGATGCCC
    GACTGTAGTC AGGTGTCTAG CAATGTTGGT GATGTCATGT CCTTTTCAGT GACTGTCACA
    GCCATCCCTG CCAGCCAGGC CGTGAATGCC GGCAGCCGCG GCCAGACCAT TCCTGTTCAG
    GCCTTCCCTG AGGAGAGCAG CGTAGAGGAC ACTCCTTCCA AGTGTTACTG CCGGTTGAAA
    GCCATGATCA TGTGCAAGGG CTGCGGAGCC TTCTGCCACG ACGATTGCAT TGGCCCCTCC
    AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691507.1
    CDS359..4711
    Translation

    Target ORF information:

    RefSeq Version XM_002691461.4
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691461.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGGTAAGG ATCTAAGGGA CTCTGGCATT
    GACACACACC AGTACCAAGA AGGACTCAGT AAGGCCACCC AAGATCAGAT TCTTCAGACT
    CTCAGCCAGA GGGTTCGGAG GCAGAACATT CTCTCATTTG TGCAGCCCTC ACAGTTCAAC
    TTTACTCACT CAGGTTTCCC GTTAGAGGAC ATTTCCACAA GCCAGAGGTT CATGCTAGGT
    TTTGCTGGCA GAAGGACATC CAAGCCTGCG ATGGCAGGTC ACTACTTACT TAATATTTCC
    ACCTATGGCC GGGGTACAGA GAGCTTTAGG AGGACCCATT CTGTGAACCC CGAAGACCGA
    TTTTGTCTGA GTAGCCCCAC TGAGGCCTTG AAAATGGGAT ACACAGACTG TAAAAATGCA
    GCAGGAGATA GTAGCAGCGG GAAGGAAGAT GATACTGACG AGGAAAGTAC TGGTGATGAG
    CAAGAATCTG TCCCGGTAAA GGAGGAGCCA CAGGCTTCTC AGAGTTCTGG CAAGTGTGAT
    GCAAGTTTAG GACCCCACAG TAGAGAAACC CTGTCCACCA GTGACTGTTC CGCTAAAAAG
    AATGTGAAGG CAGAGACACC AGTGCCTGAG CAAACCCCTT TAAGTAAGGA GAATTACCTG
    TTCACTCGAG GCCAAACCTT TGACGAGAAG ACCCTAGCCA GAGATTTTAT TCAGGCAGCA
    CAGAAGCAGA TGGCTCATGC AGTGAGAGGT AAGACCATGC GGAGCAGCCC AGAGCTTTTC
    AATTCTACGT CTCTTCCTCT GCCTGCAGAC AGTCCTACCC ATCAATCTCT CCTCCTCCCA
    CCACTGCAAA CCCCAAAGCT GTACGGGAGC CCCACACAGA TCGGGCCAAG CTACAGAGGC
    ATGATCAACG TCTCCACCTC ATCAGACATG GACCATAACT CCTCTGTCCC GGGGATGCCC
    GACTGTAGTC AGGTGTCTAG CAATGTTGGT GATGTCATGT CCTTTTCAGT GACTGTCACA
    GCCATCCCTG CCAGCCAGGC CGTGAATGCC GGCAGCCGCG GCCAGACCAT TCCTGTTCAG
    GCCTTCCCTG AGGAGAGCAG CGTAGAGGAC ACTCCTTCCA AGTGTTACTG CCGGTTGAAA
    GCCATGATCA TGTGCAAGGG CTGCGGAGCC TTCTGCCACG ACGATTGCAT TGGCCCCTCC
    AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24264
    Clone ID Related Accession (Same CDS sequence) XM_002691461.4 , XM_010816993.2 , XM_002691461.5
    Accession Version XM_010816993.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGGTAAGG ATCTAAGGGA CTCTGGCATT
    GACACACACC AGTACCAAGA AGGACTCAGT AAGGCCACCC AAGATCAGAT TCTTCAGACT
    CTCAGCCAGA GGGTTCGGAG GCAGAACATT CTCTCATTTG TGCAGCCCTC ACAGTTCAAC
    TTTACTCACT CAGGTTTCCC GTTAGAGGAC ATTTCCACAA GCCAGAGGTT CATGCTAGGT
    TTTGCTGGCA GAAGGACATC CAAGCCTGCG ATGGCAGGTC ACTACTTACT TAATATTTCC
    ACCTATGGCC GGGGTACAGA GAGCTTTAGG AGGACCCATT CTGTGAACCC CGAAGACCGA
    TTTTGTCTGA GTAGCCCCAC TGAGGCCTTG AAAATGGGAT ACACAGACTG TAAAAATGCA
    GCAGGAGATA GTAGCAGCGG GAAGGAAGAT GATACTGACG AGGAAAGTAC TGGTGATGAG
    CAAGAATCTG TCCCGGTAAA GGAGGAGCCA CAGGCTTCTC AGAGTTCTGG CAAGTGTGAT
    GCAAGTTTAG GACCCCACAG TAGAGAAACC CTGTCCACCA GTGACTGTTC CGCTAAAAAG
    AATGTGAAGG CAGAGACACC AGTGCCTGAG CAAACCCCTT TAAGTAAGGA GAATTACCTG
    TTCACTCGAG GCCAAACCTT TGACGAGAAG ACCCTAGCCA GAGATTTTAT TCAGGCAGCA
    CAGAAGCAGA TGGCTCATGC AGTGAGAGGT AAGACCATGC GGAGCAGCCC AGAGCTTTTC
    AATTCTACGT CTCTTCCTCT GCCTGCAGAC AGTCCTACCC ATCAATCTCT CCTCCTCCCA
    CCACTGCAAA CCCCAAAGCT GTACGGGAGC CCCACACAGA TCGGGCCAAG CTACAGAGGC
    ATGATCAACG TCTCCACCTC ATCAGACATG GACCATAACT CCTCTGTCCC GGGGATGCCC
    GACTGTAGTC AGGTGTCTAG CAATGTTGGT GATGTCATGT CCTTTTCAGT GACTGTCACA
    GCCATCCCTG CCAGCCAGGC CGTGAATGCC GGCAGCCGCG GCCAGACCAT TCCTGTTCAG
    GCCTTCCCTG AGGAGAGCAG CGTAGAGGAC ACTCCTTCCA AGTGTTACTG CCGGTTGAAA
    GCCATGATCA TGTGCAAGGG CTGCGGAGCC TTCTGCCACG ACGATTGCAT TGGCCCCTCC
    AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815295.2
    CDS359..4711
    Translation

    Target ORF information:

    RefSeq Version XM_010816993.2
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816993.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGGTAAGG ATCTAAGGGA CTCTGGCATT
    GACACACACC AGTACCAAGA AGGACTCAGT AAGGCCACCC AAGATCAGAT TCTTCAGACT
    CTCAGCCAGA GGGTTCGGAG GCAGAACATT CTCTCATTTG TGCAGCCCTC ACAGTTCAAC
    TTTACTCACT CAGGTTTCCC GTTAGAGGAC ATTTCCACAA GCCAGAGGTT CATGCTAGGT
    TTTGCTGGCA GAAGGACATC CAAGCCTGCG ATGGCAGGTC ACTACTTACT TAATATTTCC
    ACCTATGGCC GGGGTACAGA GAGCTTTAGG AGGACCCATT CTGTGAACCC CGAAGACCGA
    TTTTGTCTGA GTAGCCCCAC TGAGGCCTTG AAAATGGGAT ACACAGACTG TAAAAATGCA
    GCAGGAGATA GTAGCAGCGG GAAGGAAGAT GATACTGACG AGGAAAGTAC TGGTGATGAG
    CAAGAATCTG TCCCGGTAAA GGAGGAGCCA CAGGCTTCTC AGAGTTCTGG CAAGTGTGAT
    GCAAGTTTAG GACCCCACAG TAGAGAAACC CTGTCCACCA GTGACTGTTC CGCTAAAAAG
    AATGTGAAGG CAGAGACACC AGTGCCTGAG CAAACCCCTT TAAGTAAGGA GAATTACCTG
    TTCACTCGAG GCCAAACCTT TGACGAGAAG ACCCTAGCCA GAGATTTTAT TCAGGCAGCA
    CAGAAGCAGA TGGCTCATGC AGTGAGAGGT AAGACCATGC GGAGCAGCCC AGAGCTTTTC
    AATTCTACGT CTCTTCCTCT GCCTGCAGAC AGTCCTACCC ATCAATCTCT CCTCCTCCCA
    CCACTGCAAA CCCCAAAGCT GTACGGGAGC CCCACACAGA TCGGGCCAAG CTACAGAGGC
    ATGATCAACG TCTCCACCTC ATCAGACATG GACCATAACT CCTCTGTCCC GGGGATGCCC
    GACTGTAGTC AGGTGTCTAG CAATGTTGGT GATGTCATGT CCTTTTCAGT GACTGTCACA
    GCCATCCCTG CCAGCCAGGC CGTGAATGCC GGCAGCCGCG GCCAGACCAT TCCTGTTCAG
    GCCTTCCCTG AGGAGAGCAG CGTAGAGGAC ACTCCTTCCA AGTGTTACTG CCGGTTGAAA
    GCCATGATCA TGTGCAAGGG CTGCGGAGCC TTCTGCCACG ACGATTGCAT TGGCCCCTCC
    AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24264
    Clone ID Related Accession (Same CDS sequence) XM_002691461.4 , XM_010816993.2 , XM_002691461.5
    Accession Version XM_002691461.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002691461.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGGTAAGG ATCTAAGGGA CTCTGGCATT
    GACACACACC AGTACCAAGA AGGACTCAGT AAGGCCACCC AAGATCAGAT TCTTCAGACT
    CTCAGCCAGA GGGTTCGGAG GCAGAACATT CTCTCATTTG TGCAGCCCTC ACAGTTCAAC
    TTTACTCACT CAGGTTTCCC GTTAGAGGAC ATTTCCACAA GCCAGAGGTT CATGCTAGGT
    TTTGCTGGCA GAAGGACATC CAAGCCTGCG ATGGCAGGTC ACTACTTACT TAATATTTCC
    ACCTATGGCC GGGGTACAGA GAGCTTTAGG AGGACCCATT CTGTGAACCC CGAAGACCGA
    TTTTGTCTGA GTAGCCCCAC TGAGGCCTTG AAAATGGGAT ACACAGACTG TAAAAATGCA
    GCAGGAGATA GTAGCAGCGG GAAGGAAGAT GATACTGACG AGGAAAGTAC TGGTGATGAG
    CAAGAATCTG TCCCGGTAAA GGAGGAGCCA CAGGCTTCTC AGAGTTCTGG CAAGTGTGAT
    GCAAGTTTAG GACCCCACAG TAGAGAAACC CTGTCCACCA GTGACTGTTC CGCTAAAAAG
    AATGTGAAGG CAGAGACACC AGTGCCTGAG CAAACCCCTT TAAGTAAGGA GAATTACCTG
    TTCACTCGAG GCCAAACCTT TGACGAGAAG ACCCTAGCCA GAGATTTTAT TCAGGCAGCA
    CAGAAGCAGA TGGCTCATGC AGTGAGAGGT AAGACCATGC GGAGCAGCCC AGAGCTTTTC
    AATTCTACGT CTCTTCCTCT GCCTGCAGAC AGTCCTACCC ATCAATCTCT CCTCCTCCCA
    CCACTGCAAA CCCCAAAGCT GTACGGGAGC CCCACACAGA TCGGGCCAAG CTACAGAGGC
    ATGATCAACG TCTCCACCTC ATCAGACATG GACCATAACT CCTCTGTCCC GGGGATGCCC
    GACTGTAGTC AGGTGTCTAG CAATGTTGGT GATGTCATGT CCTTTTCAGT GACTGTCACA
    GCCATCCCTG CCAGCCAGGC CGTGAATGCC GGCAGCCGCG GCCAGACCAT TCCTGTTCAG
    GCCTTCCCTG AGGAGAGCAG CGTAGAGGAC ACTCCTTCCA AGTGTTACTG CCGGTTGAAA
    GCCATGATCA TGTGCAAGGG CTGCGGAGCC TTCTGCCACG ACGATTGCAT TGGCCCCTCC
    AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002691507.1
    CDS285..4637
    Translation

    Target ORF information:

    RefSeq Version XM_002691461.5
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002691461.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG AAGTGGAACT TCCCCTCTTG CATGTCTGAA TGCCATGCTG
    CACACAAACT CCCGAGGTGA AGAAGGCATC TTCTATAAGG TTCCAGGTAG AATGGGAGTA
    TATACTTTGA AGAAAGATGT GCCGGATGGG GTGAAAGAGC TTTCAGAAGG TTCAGAAGAA
    AGCAGTGATG GTCAGTCAGA TTCCCAGAGT TCTGAGAACA GCAGCAGCAG CAGTGATGGT
    GGCAGCAACA AGGAGGGAAA AAAGAGCAGG TGGAAAAGGA AAGTATCATC TAGGCTGTCA
    CAGCCATCTT CTCCTCATTC GGGCTGCCCA TCTCCTACCA TTCCAGCAGG TAAAGTCATT
    TCTTCATCAC AGAAGCACAG CAAGAAGGCA CTAAAGCAGG CACTAAAACA GCAACAACAG
    AAGAAACAGC AGCAGCAGCA ATGCAGGCCA AGCATGTCCA TCTCCTCCAA CCAGCACCTC
    TCTCTGAAGA CTGTCAAAGC ATCCAGTGAC CCTGTACCTG CCAAACCTGC GATATGGGAA
    GGAAAGCAAT CTGATGGACA GTCAAGCAGC CCTCAGAACT CAAACTCTAG CTTTTCTTCT
    TCTTCGGTTA AAGTGGAAAA TCATTTGCTG GGCCTGGGGA AGAAGTCATT CCAGAGGTCT
    GACAGGCTCC ACACAAGGCA AATGAAGAGA ACTAAATGTG CTGAGATTGA TGTTGAGACG
    CCAGATTCCA TTCTGGTTAA TACAAACCTA CGAGCGCTAA TCAACAAACA CACCTTTTCA
    GTCCTTCCTG GAGACTGCCA ACAGCGATTG CTTTTACTAC TTCCAGAGGT GGATCGACAG
    GTTGGTCCAG ATGGTCTGAT GAAGTTAAAT GGCTCAGCCC TTAATAATGA GTTCTTCACT
    TCAGCAGCCC AAGGCTGGAA GGAAAGACTT TCAGAAGGTG AGTTTACACC GGAGATGCAG
    GTGAGAATTC GACAAGAGAT TGAGAAGGAG AAGAAAGTAG AGCCGTGGAA AGAACAATTC
    TTTGAAAGCT ACTATGGTCA GAGTTCTGGC CTGAGTCTTG AAGATTCAAA GAAATTGACA
    GCTTCACCCA ATGATCCCAA AGTAAAGAAA ACCCCAGCTG AGCAACCAAA ATCTACGCTT
    CCTTCAGAGG CCTCGCCTGT CAGTGTAGTC CCAGTAATTC CCCAGTTAGA ATCTAAAGAG
    GAAGTACTGC AGATGCCATC ACCAGTCAGA AAAGAAGAGC ATGAAAGCCA AGATAAGACG
    CAGCCACACT CCACAGAGCC CCTTCTTTCC CCAGCTACCA ATACAAATGA GCTTAGCAGC
    ATTCCTCCCA TCAAGTGCCC GAAGGATGAG GCTCTTGTGG AGCAAAAGCC AGTTGCCTCT
    GCTGAACAGG AATCTGAGAA AAAGAATCAT CTCACTACAG CTTCAAATTA TAATAAAAAT
    GAAAGCCAGG AAGCTTTAGT TACTTCCCTG AGCAAACCCA AGAGTCCTGG GGTAGAAACA
    ACAGTAATGA AGCCCACAGT AGAAGCAAGT CCACAGGAGA CAACTATGAA AGAGCCTCCA
    TCAACTCTGG CTGATCACAG CCCAGAAAGC CTCAAGAGGA AATCTTCTCT CACCCAAGAA
    GAGGCCCCAA CAAGCTGGGA GAAAAGGCCG CGTGTCACTG AGCATCGCCA GCACCAGCAG
    CCATTTCAAG TCTCCCCACA GCCCTTTCTC AGTAGAGGGG ACAGGTTCCA GGTGCGGAGA
    GTACCACCTC TCAAGATCCC GGTCTCCAGA ATCTCTCCCA TGCCGTTTCC TACATCGCAG
    GTCTCTCCCA GGGCTCGTTT TCCAATCTCC ATCACTAGTC CTAACAGAAC AGGAGCCAGA
    ACCCTTGCAG ACATCAAAGC AAAAGCCCAG CTTGTCAAAG CACAGAGGGC TGCCGCCGCC
    GCCGCCGCCG CAGCCGCTGC TGCCGCCTCA GTTGGAGGGA CCATTCCAGG ACCTGGCCCA
    GGCGGTGGAC AGGGTCCAGG GGAGGGGGGT GAAAGGAGAA CTGCTAGAGG AGGGGATCCT
    GACTCAGACA AAGTCAGTGA GCCCAGCAAG GGCCCCGCAC TGGAACTGGC AGGAACTGGA
    AGCAGGGGAG GTACGAGAGA GCTTTTACCC GCTGGGCCAG ACACTCAGCC CCAGTCTGAG
    ACCAGGACCG CAGGCCAGCC ACAGCCTCTT AGTGGCCCTG GAGCACAACT ACAGCAAACC
    CCCTCAGTGC CTCCCGCATC TGCCGTCGGT GGAGCATGTG CAGGTGTCCC CTCCCCAGCC
    CACATTCATG CACTCCCGCC AGCTTTAGGA AAATTAAGTA ATGAAAAACT AAATCCCCCC
    AGGGTAACAG GCACGGTGGC CTCTCTCAGC CAACCCCAAG GGCCCAGTAA TGGTAGGCAG
    GAGAAAGCAC CTCCAGCTCC AGCAGATCCT GCTCTGATCA TGGGTGCCTC GCCTGTTCAT
    TTTGCAGCTG CTGGCGCAGT GGAGCCTAAA GCAGGTTCTA GTAAGAATGC CCCCAATCCT
    CCGGCCTCAG CAGAGATAAG TGCTAGCGCC TCAGTGGATA CAACTGCCTC CCCTTTAACA
    TCTCTGTTAA CAACAGCCAC TTTAGAGAAG CTCCCTGTGC CGCAGGTCAG CGTAACCACA
    ACGCCTACTG GATCAGCTCC ATCCTTGAGC ACTTTGCCGG CAGCCTCTAG CCTTAAAACT
    CCGGGAACTT CTTCACATAT GAATGGACCC ATTTCAAGGC CAAGCTCTAG TATCCCTGCT
    AATAATCCTT TGGTAACTCA GCTGCTGCAG GGCAAAGATG TTCCCATGGA GCAAATTCTG
    CCTAAACCTC TTACCAAAGT TGAAATGAAA ACTGTTCCAC TCACTACAAA AGAGGAGAAG
    GGAGTAGGAG CACTCATAGG TACCAGCGTG ACAGAGAACA GCACCAGAGA GGAAGTTCAT
    GAGAGACAGT CCCATCCAGC TGCACAGCAC CTGGGTAAAA CTTTGCAAAG TAAGCAGCTC
    CCCCAGGTTC CAAGACCCCT GCAGCTCTTT TCAGGTAAGG ATCTAAGGGA CTCTGGCATT
    GACACACACC AGTACCAAGA AGGACTCAGT AAGGCCACCC AAGATCAGAT TCTTCAGACT
    CTCAGCCAGA GGGTTCGGAG GCAGAACATT CTCTCATTTG TGCAGCCCTC ACAGTTCAAC
    TTTACTCACT CAGGTTTCCC GTTAGAGGAC ATTTCCACAA GCCAGAGGTT CATGCTAGGT
    TTTGCTGGCA GAAGGACATC CAAGCCTGCG ATGGCAGGTC ACTACTTACT TAATATTTCC
    ACCTATGGCC GGGGTACAGA GAGCTTTAGG AGGACCCATT CTGTGAACCC CGAAGACCGA
    TTTTGTCTGA GTAGCCCCAC TGAGGCCTTG AAAATGGGAT ACACAGACTG TAAAAATGCA
    GCAGGAGATA GTAGCAGCGG GAAGGAAGAT GATACTGACG AGGAAAGTAC TGGTGATGAG
    CAAGAATCTG TCCCGGTAAA GGAGGAGCCA CAGGCTTCTC AGAGTTCTGG CAAGTGTGAT
    GCAAGTTTAG GACCCCACAG TAGAGAAACC CTGTCCACCA GTGACTGTTC CGCTAAAAAG
    AATGTGAAGG CAGAGACACC AGTGCCTGAG CAAACCCCTT TAAGTAAGGA GAATTACCTG
    TTCACTCGAG GCCAAACCTT TGACGAGAAG ACCCTAGCCA GAGATTTTAT TCAGGCAGCA
    CAGAAGCAGA TGGCTCATGC AGTGAGAGGT AAGACCATGC GGAGCAGCCC AGAGCTTTTC
    AATTCTACGT CTCTTCCTCT GCCTGCAGAC AGTCCTACCC ATCAATCTCT CCTCCTCCCA
    CCACTGCAAA CCCCAAAGCT GTACGGGAGC CCCACACAGA TCGGGCCAAG CTACAGAGGC
    ATGATCAACG TCTCCACCTC ATCAGACATG GACCATAACT CCTCTGTCCC GGGGATGCCC
    GACTGTAGTC AGGTGTCTAG CAATGTTGGT GATGTCATGT CCTTTTCAGT GACTGTCACA
    GCCATCCCTG CCAGCCAGGC CGTGAATGCC GGCAGCCGCG GCCAGACCAT TCCTGTTCAG
    GCCTTCCCTG AGGAGAGCAG CGTAGAGGAC ACTCCTTCCA AGTGTTACTG CCGGTTGAAA
    GCCATGATCA TGTGCAAGGG CTGCGGAGCC TTCTGCCACG ACGATTGCAT TGGCCCCTCC
    AAACTCTGTG TCTCCTGCCT TGTTGTTCGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24265
    Clone ID Related Accession (Same CDS sequence) XM_005213080.4 , XM_005193938.3 , XM_005213080.3
    Accession Version XM_005213080.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213080.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG TGGAACTTCC CCTCTTGCAT GTCTGAATGC CATGCTGCAC
    ACAAACTCCC GAGGTGAAGA AGGCATCTTC TATAAGGTTC CAGGTAGAAT GGGAGTATAT
    ACTTTGAAGA AAGATGTGCC GGATGGGGTG AAAGAGCTTT CAGAAGGTTC AGAAGAAAGC
    AGTGATGGTC AGTCAGATTC CCAGAGTTCT GAGAACAGCA GCAGCAGCAG TGATGGTGGC
    AGCAACAAGG AGGGAAAAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG GCTGTCACAG
    CCATCTTCTC CTCATTCGGG CTGCCCATCT CCTACCATTC CAGCAGGTAA AGTCATTTCT
    TCATCACAGA AGCACAGCAA GAAGGCACTA AAGCAGGCAC TAAAACAGCA ACAACAGAAG
    AAACAGCAGC AGCAGCAATG CAGGCCAAGC ATGTCCATCT CCTCCAACCA GCACCTCTCT
    CTGAAGACTG TCAAAGCATC CAGTGACCCT GTACCTGCCA AACCTGCGAT ATGGGAAGGA
    AAGCAATCTG ATGGACAGTC AAGCAGCCCT CAGAACTCAA ACTCTAGCTT TTCTTCTTCT
    TCGGTTAAAG TGGAAAATCA TTTGCTGGGC CTGGGGAAGA AGTCATTCCA GAGGTCTGAC
    AGGCTCCACA CAAGGCAAAT GAAGAGAACT AAATGTGCTG AGATTGATGT TGAGACGCCA
    GATTCCATTC TGGTTAATAC AAACCTACGA GCGCTAATCA ACAAACACAC CTTTTCAGTC
    CTTCCTGGAG ACTGCCAACA GCGATTGCTT TTACTACTTC CAGAGGTGGA TCGACAGGTT
    GGTCCAGATG GTCTGATGAA GTTAAATGGC TCAGCCCTTA ATAATGAGTT CTTCACTTCA
    GCAGCCCAAG GCTGGAAGGA AAGACTTTCA GAAGGTGAGT TTACACCGGA GATGCAGGTG
    AGAATTCGAC AAGAGATTGA GAAGGAGAAG AAAGTAGAGC CGTGGAAAGA ACAATTCTTT
    GAAAGCTACT ATGGTCAGAG TTCTGGCCTG AGTCTTGAAG ATTCAAAGAA ATTGACAGCT
    TCACCCAATG ATCCCAAAGT AAAGAAAACC CCAGCTGAGC AACCAAAATC TACGCTTCCT
    TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA GTAATTCCCC AGTTAGAATC TAAAGAGGAA
    GTACTGCAGA TGCCATCACC AGTCAGAAAA GAAGAGCATG AAAGCCAAGA TAAGACGCAG
    CCACACTCCA CAGAGCCCCT TCTTTCCCCA GCTACCAATA CAAATGAGCT TAGCAGCATT
    CCTCCCATCA AGTGCCCGAA GGATGAGGCT CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT
    GAACAGGAAT CTGAGAAAAA GAATCATCTC ACTACAGCTT CAAATTATAA TAAAAATGAA
    AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC AAACCCAAGA GTCCTGGGGT AGAAACAACA
    GTAATGAAGC CCACAGTAGA AGCAAGTCCA CAGGAGACAA CTATGAAAGA GCCTCCATCA
    ACTCTGGCTG ATCACAGCCC AGAAAGCCTC AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG
    GCCCCAACAA GCTGGGAGAA AAGGCCGCGT GTCACTGAGC ATCGCCAGCA CCAGCAGCCA
    TTTCAAGTCT CCCCACAGCC CTTTCTCAGT AGAGGGGACA GGTTCCAGGT GCGGAGAGTA
    CCACCTCTCA AGATCCCGGT CTCCAGAATC TCTCCCATGC CGTTTCCTAC ATCGCAGGTC
    TCTCCCAGGG CTCGTTTTCC AATCTCCATC ACTAGTCCTA ACAGAACAGG AGCCAGAACC
    CTTGCAGACA TCAAAGCAAA AGCCCAGCTT GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC
    GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT GGAGGGACCA TTCCAGGACC TGGCCCAGGC
    GGTGGACAGG GTCCAGGGGA GGGGGGTGAA AGGAGAACTG CTAGAGGAGG GGATCCTGAC
    TCAGACAAAG TCAGTGAGCC CAGCAAGGGC CCCGCACTGG AACTGGCAGG AACTGGAAGC
    AGGGGAGGTA CGAGAGAGCT TTTACCCGCT GGGCCAGACA CTCAGCCCCA GTCTGAGACC
    AGGACCGCAG GCCAGCCACA GCCTCTTAGT GGCCCTGGAG CACAACTACA GCAAACCCCC
    TCAGTGCCTC CCGCATCTGC CGTCGGTGGA GCATGTGCAG GTGTCCCCTC CCCAGCCCAC
    ATTCATGCAC TCCCGCCAGC TTTAGGAAAA TTAAGTAATG AAAAACTAAA TCCCCCCAGG
    GTAACAGGCA CGGTGGCCTC TCTCAGCCAA CCCCAAGGGC CCAGTAATGG TAGGCAGGAG
    AAAGCACCTC CAGCTCCAGC AGATCCTGCT CTGATCATGG GTGCCTCGCC TGTTCATTTT
    GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA GGTTCTAGTA AGAATGCCCC CAATCCTCCG
    GCCTCAGCAG AGATAAGTGC TAGCGCCTCA GTGGATACAA CTGCCTCCCC TTTAACATCT
    CTGTTAACAA CAGCCACTTT AGAGAAGCTC CCTGTGCCGC AGGTCAGCGT AACCACAACG
    CCTACTGGAT CAGCTCCATC CTTGAGCACT TTGCCGGCAG CCTCTAGCCT TAAAACTCCG
    GGAACTTCTT CACATATGAA TGGACCCATT TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT
    AATCCTTTGG TAACTCAGCT GCTGCAGGGC AAAGATGTTC CCATGGAGCA AATTCTGCCT
    AAACCTCTTA CCAAAGTTGA AATGAAAACT GTTCCACTCA CTACAAAAGA GGAGAAGGGA
    GTAGGAGCAC TCATAGGTAC CAGCGTGACA GAGAACAGCA CCAGAGAGGA AGTTCATGAG
    AGACAGTCCC ATCCAGCTGC ACAGCACCTG GGTAAAACTT TGCAAAGTAA GCAGCTCCCC
    CAGGTTCCAA GACCCCTGCA GCTCTTTTCA GGTAAGGATC TAAGGGACTC TGGCATTGAC
    ACACACCAGT ACCAAGAAGG ACTCAGTAAG GCCACCCAAG ATCAGATTCT TCAGACTCTC
    AGCCAGAGGG TTCGGAGGCA GAACATTCTC TCATTTGTGC AGCCCTCACA GTTCAACTTT
    ACTCACTCAG GTTTCCCGTT AGAGGACATT TCCACAAGCC AGAGGTTCAT GCTAGGTTTT
    GCTGGCAGAA GGACATCCAA GCCTGCGATG GCAGGTCACT ACTTACTTAA TATTTCCACC
    TATGGCCGGG GTACAGAGAG CTTTAGGAGG ACCCATTCTG TGAACCCCGA AGACCGATTT
    TGTCTGAGTA GCCCCACTGA GGCCTTGAAA ATGGGATACA CAGACTGTAA AAATGCAGCA
    GGAGATAGTA GCAGCGGGAA GGAAGATGAT ACTGACGAGG AAAGTACTGG TGATGAGCAA
    GAATCTGTCC CGGTAAAGGA GGAGCCACAG GCTTCTCAGA GTTCTGGCAA GTGTGATGCA
    AGTTTAGGAC CCCACAGTAG AGAAACCCTG TCCACCAGTG ACTGTTCCGC TAAAAAGAAT
    GTGAAGGCAG AGACACCAGT GCCTGAGCAA ACCCCTTTAA GTAAGGAGAA TTACCTGTTC
    ACTCGAGGCC AAACCTTTGA CGAGAAGACC CTAGCCAGAG ATTTTATTCA GGCAGCACAG
    AAGCAGATGG CTCATGCAGT GAGAGGTAAG ACCATGCGGA GCAGCCCAGA GCTTTTCAAT
    TCTACGTCTC TTCCTCTGCC TGCAGACAGT CCTACCCATC AATCTCTCCT CCTCCCACCA
    CTGCAAACCC CAAAGCTGTA CGGGAGCCCC ACACAGATCG GGCCAAGCTA CAGAGGCATG
    ATCAACGTCT CCACCTCATC AGACATGGAC CATAACTCCT CTGTCCCGGG GATGCCCGAC
    TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT GTCATGTCCT TTTCAGTGAC TGTCACAGCC
    ATCCCTGCCA GCCAGGCCGT GAATGCCGGC AGCCGCGGCC AGACCATTCC TGTTCAGGCC
    TTCCCTGAGG AGAGCAGCGT AGAGGACACT CCTTCCAAGT GTTACTGCCG GTTGAAAGCC
    ATGATCATGT GCAAGGGCTG CGGAGCCTTC TGCCACGACG ATTGCATTGG CCCCTCCAAA
    CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213137.1
    CDS238..4587
    Translation

    Target ORF information:

    RefSeq Version XM_005213080.4
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213080.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG TGGAACTTCC CCTCTTGCAT GTCTGAATGC CATGCTGCAC
    ACAAACTCCC GAGGTGAAGA AGGCATCTTC TATAAGGTTC CAGGTAGAAT GGGAGTATAT
    ACTTTGAAGA AAGATGTGCC GGATGGGGTG AAAGAGCTTT CAGAAGGTTC AGAAGAAAGC
    AGTGATGGTC AGTCAGATTC CCAGAGTTCT GAGAACAGCA GCAGCAGCAG TGATGGTGGC
    AGCAACAAGG AGGGAAAAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG GCTGTCACAG
    CCATCTTCTC CTCATTCGGG CTGCCCATCT CCTACCATTC CAGCAGGTAA AGTCATTTCT
    TCATCACAGA AGCACAGCAA GAAGGCACTA AAGCAGGCAC TAAAACAGCA ACAACAGAAG
    AAACAGCAGC AGCAGCAATG CAGGCCAAGC ATGTCCATCT CCTCCAACCA GCACCTCTCT
    CTGAAGACTG TCAAAGCATC CAGTGACCCT GTACCTGCCA AACCTGCGAT ATGGGAAGGA
    AAGCAATCTG ATGGACAGTC AAGCAGCCCT CAGAACTCAA ACTCTAGCTT TTCTTCTTCT
    TCGGTTAAAG TGGAAAATCA TTTGCTGGGC CTGGGGAAGA AGTCATTCCA GAGGTCTGAC
    AGGCTCCACA CAAGGCAAAT GAAGAGAACT AAATGTGCTG AGATTGATGT TGAGACGCCA
    GATTCCATTC TGGTTAATAC AAACCTACGA GCGCTAATCA ACAAACACAC CTTTTCAGTC
    CTTCCTGGAG ACTGCCAACA GCGATTGCTT TTACTACTTC CAGAGGTGGA TCGACAGGTT
    GGTCCAGATG GTCTGATGAA GTTAAATGGC TCAGCCCTTA ATAATGAGTT CTTCACTTCA
    GCAGCCCAAG GCTGGAAGGA AAGACTTTCA GAAGGTGAGT TTACACCGGA GATGCAGGTG
    AGAATTCGAC AAGAGATTGA GAAGGAGAAG AAAGTAGAGC CGTGGAAAGA ACAATTCTTT
    GAAAGCTACT ATGGTCAGAG TTCTGGCCTG AGTCTTGAAG ATTCAAAGAA ATTGACAGCT
    TCACCCAATG ATCCCAAAGT AAAGAAAACC CCAGCTGAGC AACCAAAATC TACGCTTCCT
    TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA GTAATTCCCC AGTTAGAATC TAAAGAGGAA
    GTACTGCAGA TGCCATCACC AGTCAGAAAA GAAGAGCATG AAAGCCAAGA TAAGACGCAG
    CCACACTCCA CAGAGCCCCT TCTTTCCCCA GCTACCAATA CAAATGAGCT TAGCAGCATT
    CCTCCCATCA AGTGCCCGAA GGATGAGGCT CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT
    GAACAGGAAT CTGAGAAAAA GAATCATCTC ACTACAGCTT CAAATTATAA TAAAAATGAA
    AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC AAACCCAAGA GTCCTGGGGT AGAAACAACA
    GTAATGAAGC CCACAGTAGA AGCAAGTCCA CAGGAGACAA CTATGAAAGA GCCTCCATCA
    ACTCTGGCTG ATCACAGCCC AGAAAGCCTC AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG
    GCCCCAACAA GCTGGGAGAA AAGGCCGCGT GTCACTGAGC ATCGCCAGCA CCAGCAGCCA
    TTTCAAGTCT CCCCACAGCC CTTTCTCAGT AGAGGGGACA GGTTCCAGGT GCGGAGAGTA
    CCACCTCTCA AGATCCCGGT CTCCAGAATC TCTCCCATGC CGTTTCCTAC ATCGCAGGTC
    TCTCCCAGGG CTCGTTTTCC AATCTCCATC ACTAGTCCTA ACAGAACAGG AGCCAGAACC
    CTTGCAGACA TCAAAGCAAA AGCCCAGCTT GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC
    GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT GGAGGGACCA TTCCAGGACC TGGCCCAGGC
    GGTGGACAGG GTCCAGGGGA GGGGGGTGAA AGGAGAACTG CTAGAGGAGG GGATCCTGAC
    TCAGACAAAG TCAGTGAGCC CAGCAAGGGC CCCGCACTGG AACTGGCAGG AACTGGAAGC
    AGGGGAGGTA CGAGAGAGCT TTTACCCGCT GGGCCAGACA CTCAGCCCCA GTCTGAGACC
    AGGACCGCAG GCCAGCCACA GCCTCTTAGT GGCCCTGGAG CACAACTACA GCAAACCCCC
    TCAGTGCCTC CCGCATCTGC CGTCGGTGGA GCATGTGCAG GTGTCCCCTC CCCAGCCCAC
    ATTCATGCAC TCCCGCCAGC TTTAGGAAAA TTAAGTAATG AAAAACTAAA TCCCCCCAGG
    GTAACAGGCA CGGTGGCCTC TCTCAGCCAA CCCCAAGGGC CCAGTAATGG TAGGCAGGAG
    AAAGCACCTC CAGCTCCAGC AGATCCTGCT CTGATCATGG GTGCCTCGCC TGTTCATTTT
    GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA GGTTCTAGTA AGAATGCCCC CAATCCTCCG
    GCCTCAGCAG AGATAAGTGC TAGCGCCTCA GTGGATACAA CTGCCTCCCC TTTAACATCT
    CTGTTAACAA CAGCCACTTT AGAGAAGCTC CCTGTGCCGC AGGTCAGCGT AACCACAACG
    CCTACTGGAT CAGCTCCATC CTTGAGCACT TTGCCGGCAG CCTCTAGCCT TAAAACTCCG
    GGAACTTCTT CACATATGAA TGGACCCATT TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT
    AATCCTTTGG TAACTCAGCT GCTGCAGGGC AAAGATGTTC CCATGGAGCA AATTCTGCCT
    AAACCTCTTA CCAAAGTTGA AATGAAAACT GTTCCACTCA CTACAAAAGA GGAGAAGGGA
    GTAGGAGCAC TCATAGGTAC CAGCGTGACA GAGAACAGCA CCAGAGAGGA AGTTCATGAG
    AGACAGTCCC ATCCAGCTGC ACAGCACCTG GGTAAAACTT TGCAAAGTAA GCAGCTCCCC
    CAGGTTCCAA GACCCCTGCA GCTCTTTTCA GGTAAGGATC TAAGGGACTC TGGCATTGAC
    ACACACCAGT ACCAAGAAGG ACTCAGTAAG GCCACCCAAG ATCAGATTCT TCAGACTCTC
    AGCCAGAGGG TTCGGAGGCA GAACATTCTC TCATTTGTGC AGCCCTCACA GTTCAACTTT
    ACTCACTCAG GTTTCCCGTT AGAGGACATT TCCACAAGCC AGAGGTTCAT GCTAGGTTTT
    GCTGGCAGAA GGACATCCAA GCCTGCGATG GCAGGTCACT ACTTACTTAA TATTTCCACC
    TATGGCCGGG GTACAGAGAG CTTTAGGAGG ACCCATTCTG TGAACCCCGA AGACCGATTT
    TGTCTGAGTA GCCCCACTGA GGCCTTGAAA ATGGGATACA CAGACTGTAA AAATGCAGCA
    GGAGATAGTA GCAGCGGGAA GGAAGATGAT ACTGACGAGG AAAGTACTGG TGATGAGCAA
    GAATCTGTCC CGGTAAAGGA GGAGCCACAG GCTTCTCAGA GTTCTGGCAA GTGTGATGCA
    AGTTTAGGAC CCCACAGTAG AGAAACCCTG TCCACCAGTG ACTGTTCCGC TAAAAAGAAT
    GTGAAGGCAG AGACACCAGT GCCTGAGCAA ACCCCTTTAA GTAAGGAGAA TTACCTGTTC
    ACTCGAGGCC AAACCTTTGA CGAGAAGACC CTAGCCAGAG ATTTTATTCA GGCAGCACAG
    AAGCAGATGG CTCATGCAGT GAGAGGTAAG ACCATGCGGA GCAGCCCAGA GCTTTTCAAT
    TCTACGTCTC TTCCTCTGCC TGCAGACAGT CCTACCCATC AATCTCTCCT CCTCCCACCA
    CTGCAAACCC CAAAGCTGTA CGGGAGCCCC ACACAGATCG GGCCAAGCTA CAGAGGCATG
    ATCAACGTCT CCACCTCATC AGACATGGAC CATAACTCCT CTGTCCCGGG GATGCCCGAC
    TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT GTCATGTCCT TTTCAGTGAC TGTCACAGCC
    ATCCCTGCCA GCCAGGCCGT GAATGCCGGC AGCCGCGGCC AGACCATTCC TGTTCAGGCC
    TTCCCTGAGG AGAGCAGCGT AGAGGACACT CCTTCCAAGT GTTACTGCCG GTTGAAAGCC
    ATGATCATGT GCAAGGGCTG CGGAGCCTTC TGCCACGACG ATTGCATTGG CCCCTCCAAA
    CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24265
    Clone ID Related Accession (Same CDS sequence) XM_005213080.4 , XM_005193938.3 , XM_005213080.3
    Accession Version XM_005193938.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193938.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG TGGAACTTCC CCTCTTGCAT GTCTGAATGC CATGCTGCAC
    ACAAACTCCC GAGGTGAAGA AGGCATCTTC TATAAGGTTC CAGGTAGAAT GGGAGTATAT
    ACTTTGAAGA AAGATGTGCC GGATGGGGTG AAAGAGCTTT CAGAAGGTTC AGAAGAAAGC
    AGTGATGGTC AGTCAGATTC CCAGAGTTCT GAGAACAGCA GCAGCAGCAG TGATGGTGGC
    AGCAACAAGG AGGGAAAAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG GCTGTCACAG
    CCATCTTCTC CTCATTCGGG CTGCCCATCT CCTACCATTC CAGCAGGTAA AGTCATTTCT
    TCATCACAGA AGCACAGCAA GAAGGCACTA AAGCAGGCAC TAAAACAGCA ACAACAGAAG
    AAACAGCAGC AGCAGCAATG CAGGCCAAGC ATGTCCATCT CCTCCAACCA GCACCTCTCT
    CTGAAGACTG TCAAAGCATC CAGTGACCCT GTACCTGCCA AACCTGCGAT ATGGGAAGGA
    AAGCAATCTG ATGGACAGTC AAGCAGCCCT CAGAACTCAA ACTCTAGCTT TTCTTCTTCT
    TCGGTTAAAG TGGAAAATCA TTTGCTGGGC CTGGGGAAGA AGTCATTCCA GAGGTCTGAC
    AGGCTCCACA CAAGGCAAAT GAAGAGAACT AAATGTGCTG AGATTGATGT TGAGACGCCA
    GATTCCATTC TGGTTAATAC AAACCTACGA GCGCTAATCA ACAAACACAC CTTTTCAGTC
    CTTCCTGGAG ACTGCCAACA GCGATTGCTT TTACTACTTC CAGAGGTGGA TCGACAGGTT
    GGTCCAGATG GTCTGATGAA GTTAAATGGC TCAGCCCTTA ATAATGAGTT CTTCACTTCA
    GCAGCCCAAG GCTGGAAGGA AAGACTTTCA GAAGGTGAGT TTACACCGGA GATGCAGGTG
    AGAATTCGAC AAGAGATTGA GAAGGAGAAG AAAGTAGAGC CGTGGAAAGA ACAATTCTTT
    GAAAGCTACT ATGGTCAGAG TTCTGGCCTG AGTCTTGAAG ATTCAAAGAA ATTGACAGCT
    TCACCCAATG ATCCCAAAGT AAAGAAAACC CCAGCTGAGC AACCAAAATC TACGCTTCCT
    TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA GTAATTCCCC AGTTAGAATC TAAAGAGGAA
    GTACTGCAGA TGCCATCACC AGTCAGAAAA GAAGAGCATG AAAGCCAAGA TAAGACGCAG
    CCACACTCCA CAGAGCCCCT TCTTTCCCCA GCTACCAATA CAAATGAGCT TAGCAGCATT
    CCTCCCATCA AGTGCCCGAA GGATGAGGCT CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT
    GAACAGGAAT CTGAGAAAAA GAATCATCTC ACTACAGCTT CAAATTATAA TAAAAATGAA
    AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC AAACCCAAGA GTCCTGGGGT AGAAACAACA
    GTAATGAAGC CCACAGTAGA AGCAAGTCCA CAGGAGACAA CTATGAAAGA GCCTCCATCA
    ACTCTGGCTG ATCACAGCCC AGAAAGCCTC AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG
    GCCCCAACAA GCTGGGAGAA AAGGCCGCGT GTCACTGAGC ATCGCCAGCA CCAGCAGCCA
    TTTCAAGTCT CCCCACAGCC CTTTCTCAGT AGAGGGGACA GGTTCCAGGT GCGGAGAGTA
    CCACCTCTCA AGATCCCGGT CTCCAGAATC TCTCCCATGC CGTTTCCTAC ATCGCAGGTC
    TCTCCCAGGG CTCGTTTTCC AATCTCCATC ACTAGTCCTA ACAGAACAGG AGCCAGAACC
    CTTGCAGACA TCAAAGCAAA AGCCCAGCTT GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC
    GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT GGAGGGACCA TTCCAGGACC TGGCCCAGGC
    GGTGGACAGG GTCCAGGGGA GGGGGGTGAA AGGAGAACTG CTAGAGGAGG GGATCCTGAC
    TCAGACAAAG TCAGTGAGCC CAGCAAGGGC CCCGCACTGG AACTGGCAGG AACTGGAAGC
    AGGGGAGGTA CGAGAGAGCT TTTACCCGCT GGGCCAGACA CTCAGCCCCA GTCTGAGACC
    AGGACCGCAG GCCAGCCACA GCCTCTTAGT GGCCCTGGAG CACAACTACA GCAAACCCCC
    TCAGTGCCTC CCGCATCTGC CGTCGGTGGA GCATGTGCAG GTGTCCCCTC CCCAGCCCAC
    ATTCATGCAC TCCCGCCAGC TTTAGGAAAA TTAAGTAATG AAAAACTAAA TCCCCCCAGG
    GTAACAGGCA CGGTGGCCTC TCTCAGCCAA CCCCAAGGGC CCAGTAATGG TAGGCAGGAG
    AAAGCACCTC CAGCTCCAGC AGATCCTGCT CTGATCATGG GTGCCTCGCC TGTTCATTTT
    GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA GGTTCTAGTA AGAATGCCCC CAATCCTCCG
    GCCTCAGCAG AGATAAGTGC TAGCGCCTCA GTGGATACAA CTGCCTCCCC TTTAACATCT
    CTGTTAACAA CAGCCACTTT AGAGAAGCTC CCTGTGCCGC AGGTCAGCGT AACCACAACG
    CCTACTGGAT CAGCTCCATC CTTGAGCACT TTGCCGGCAG CCTCTAGCCT TAAAACTCCG
    GGAACTTCTT CACATATGAA TGGACCCATT TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT
    AATCCTTTGG TAACTCAGCT GCTGCAGGGC AAAGATGTTC CCATGGAGCA AATTCTGCCT
    AAACCTCTTA CCAAAGTTGA AATGAAAACT GTTCCACTCA CTACAAAAGA GGAGAAGGGA
    GTAGGAGCAC TCATAGGTAC CAGCGTGACA GAGAACAGCA CCAGAGAGGA AGTTCATGAG
    AGACAGTCCC ATCCAGCTGC ACAGCACCTG GGTAAAACTT TGCAAAGTAA GCAGCTCCCC
    CAGGTTCCAA GACCCCTGCA GCTCTTTTCA GGTAAGGATC TAAGGGACTC TGGCATTGAC
    ACACACCAGT ACCAAGAAGG ACTCAGTAAG GCCACCCAAG ATCAGATTCT TCAGACTCTC
    AGCCAGAGGG TTCGGAGGCA GAACATTCTC TCATTTGTGC AGCCCTCACA GTTCAACTTT
    ACTCACTCAG GTTTCCCGTT AGAGGACATT TCCACAAGCC AGAGGTTCAT GCTAGGTTTT
    GCTGGCAGAA GGACATCCAA GCCTGCGATG GCAGGTCACT ACTTACTTAA TATTTCCACC
    TATGGCCGGG GTACAGAGAG CTTTAGGAGG ACCCATTCTG TGAACCCCGA AGACCGATTT
    TGTCTGAGTA GCCCCACTGA GGCCTTGAAA ATGGGATACA CAGACTGTAA AAATGCAGCA
    GGAGATAGTA GCAGCGGGAA GGAAGATGAT ACTGACGAGG AAAGTACTGG TGATGAGCAA
    GAATCTGTCC CGGTAAAGGA GGAGCCACAG GCTTCTCAGA GTTCTGGCAA GTGTGATGCA
    AGTTTAGGAC CCCACAGTAG AGAAACCCTG TCCACCAGTG ACTGTTCCGC TAAAAAGAAT
    GTGAAGGCAG AGACACCAGT GCCTGAGCAA ACCCCTTTAA GTAAGGAGAA TTACCTGTTC
    ACTCGAGGCC AAACCTTTGA CGAGAAGACC CTAGCCAGAG ATTTTATTCA GGCAGCACAG
    AAGCAGATGG CTCATGCAGT GAGAGGTAAG ACCATGCGGA GCAGCCCAGA GCTTTTCAAT
    TCTACGTCTC TTCCTCTGCC TGCAGACAGT CCTACCCATC AATCTCTCCT CCTCCCACCA
    CTGCAAACCC CAAAGCTGTA CGGGAGCCCC ACACAGATCG GGCCAAGCTA CAGAGGCATG
    ATCAACGTCT CCACCTCATC AGACATGGAC CATAACTCCT CTGTCCCGGG GATGCCCGAC
    TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT GTCATGTCCT TTTCAGTGAC TGTCACAGCC
    ATCCCTGCCA GCCAGGCCGT GAATGCCGGC AGCCGCGGCC AGACCATTCC TGTTCAGGCC
    TTCCCTGAGG AGAGCAGCGT AGAGGACACT CCTTCCAAGT GTTACTGCCG GTTGAAAGCC
    ATGATCATGT GCAAGGGCTG CGGAGCCTTC TGCCACGACG ATTGCATTGG CCCCTCCAAA
    CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005193995.2
    CDS359..4708
    Translation

    Target ORF information:

    RefSeq Version XM_005193938.3
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193938.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG TGGAACTTCC CCTCTTGCAT GTCTGAATGC CATGCTGCAC
    ACAAACTCCC GAGGTGAAGA AGGCATCTTC TATAAGGTTC CAGGTAGAAT GGGAGTATAT
    ACTTTGAAGA AAGATGTGCC GGATGGGGTG AAAGAGCTTT CAGAAGGTTC AGAAGAAAGC
    AGTGATGGTC AGTCAGATTC CCAGAGTTCT GAGAACAGCA GCAGCAGCAG TGATGGTGGC
    AGCAACAAGG AGGGAAAAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG GCTGTCACAG
    CCATCTTCTC CTCATTCGGG CTGCCCATCT CCTACCATTC CAGCAGGTAA AGTCATTTCT
    TCATCACAGA AGCACAGCAA GAAGGCACTA AAGCAGGCAC TAAAACAGCA ACAACAGAAG
    AAACAGCAGC AGCAGCAATG CAGGCCAAGC ATGTCCATCT CCTCCAACCA GCACCTCTCT
    CTGAAGACTG TCAAAGCATC CAGTGACCCT GTACCTGCCA AACCTGCGAT ATGGGAAGGA
    AAGCAATCTG ATGGACAGTC AAGCAGCCCT CAGAACTCAA ACTCTAGCTT TTCTTCTTCT
    TCGGTTAAAG TGGAAAATCA TTTGCTGGGC CTGGGGAAGA AGTCATTCCA GAGGTCTGAC
    AGGCTCCACA CAAGGCAAAT GAAGAGAACT AAATGTGCTG AGATTGATGT TGAGACGCCA
    GATTCCATTC TGGTTAATAC AAACCTACGA GCGCTAATCA ACAAACACAC CTTTTCAGTC
    CTTCCTGGAG ACTGCCAACA GCGATTGCTT TTACTACTTC CAGAGGTGGA TCGACAGGTT
    GGTCCAGATG GTCTGATGAA GTTAAATGGC TCAGCCCTTA ATAATGAGTT CTTCACTTCA
    GCAGCCCAAG GCTGGAAGGA AAGACTTTCA GAAGGTGAGT TTACACCGGA GATGCAGGTG
    AGAATTCGAC AAGAGATTGA GAAGGAGAAG AAAGTAGAGC CGTGGAAAGA ACAATTCTTT
    GAAAGCTACT ATGGTCAGAG TTCTGGCCTG AGTCTTGAAG ATTCAAAGAA ATTGACAGCT
    TCACCCAATG ATCCCAAAGT AAAGAAAACC CCAGCTGAGC AACCAAAATC TACGCTTCCT
    TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA GTAATTCCCC AGTTAGAATC TAAAGAGGAA
    GTACTGCAGA TGCCATCACC AGTCAGAAAA GAAGAGCATG AAAGCCAAGA TAAGACGCAG
    CCACACTCCA CAGAGCCCCT TCTTTCCCCA GCTACCAATA CAAATGAGCT TAGCAGCATT
    CCTCCCATCA AGTGCCCGAA GGATGAGGCT CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT
    GAACAGGAAT CTGAGAAAAA GAATCATCTC ACTACAGCTT CAAATTATAA TAAAAATGAA
    AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC AAACCCAAGA GTCCTGGGGT AGAAACAACA
    GTAATGAAGC CCACAGTAGA AGCAAGTCCA CAGGAGACAA CTATGAAAGA GCCTCCATCA
    ACTCTGGCTG ATCACAGCCC AGAAAGCCTC AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG
    GCCCCAACAA GCTGGGAGAA AAGGCCGCGT GTCACTGAGC ATCGCCAGCA CCAGCAGCCA
    TTTCAAGTCT CCCCACAGCC CTTTCTCAGT AGAGGGGACA GGTTCCAGGT GCGGAGAGTA
    CCACCTCTCA AGATCCCGGT CTCCAGAATC TCTCCCATGC CGTTTCCTAC ATCGCAGGTC
    TCTCCCAGGG CTCGTTTTCC AATCTCCATC ACTAGTCCTA ACAGAACAGG AGCCAGAACC
    CTTGCAGACA TCAAAGCAAA AGCCCAGCTT GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC
    GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT GGAGGGACCA TTCCAGGACC TGGCCCAGGC
    GGTGGACAGG GTCCAGGGGA GGGGGGTGAA AGGAGAACTG CTAGAGGAGG GGATCCTGAC
    TCAGACAAAG TCAGTGAGCC CAGCAAGGGC CCCGCACTGG AACTGGCAGG AACTGGAAGC
    AGGGGAGGTA CGAGAGAGCT TTTACCCGCT GGGCCAGACA CTCAGCCCCA GTCTGAGACC
    AGGACCGCAG GCCAGCCACA GCCTCTTAGT GGCCCTGGAG CACAACTACA GCAAACCCCC
    TCAGTGCCTC CCGCATCTGC CGTCGGTGGA GCATGTGCAG GTGTCCCCTC CCCAGCCCAC
    ATTCATGCAC TCCCGCCAGC TTTAGGAAAA TTAAGTAATG AAAAACTAAA TCCCCCCAGG
    GTAACAGGCA CGGTGGCCTC TCTCAGCCAA CCCCAAGGGC CCAGTAATGG TAGGCAGGAG
    AAAGCACCTC CAGCTCCAGC AGATCCTGCT CTGATCATGG GTGCCTCGCC TGTTCATTTT
    GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA GGTTCTAGTA AGAATGCCCC CAATCCTCCG
    GCCTCAGCAG AGATAAGTGC TAGCGCCTCA GTGGATACAA CTGCCTCCCC TTTAACATCT
    CTGTTAACAA CAGCCACTTT AGAGAAGCTC CCTGTGCCGC AGGTCAGCGT AACCACAACG
    CCTACTGGAT CAGCTCCATC CTTGAGCACT TTGCCGGCAG CCTCTAGCCT TAAAACTCCG
    GGAACTTCTT CACATATGAA TGGACCCATT TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT
    AATCCTTTGG TAACTCAGCT GCTGCAGGGC AAAGATGTTC CCATGGAGCA AATTCTGCCT
    AAACCTCTTA CCAAAGTTGA AATGAAAACT GTTCCACTCA CTACAAAAGA GGAGAAGGGA
    GTAGGAGCAC TCATAGGTAC CAGCGTGACA GAGAACAGCA CCAGAGAGGA AGTTCATGAG
    AGACAGTCCC ATCCAGCTGC ACAGCACCTG GGTAAAACTT TGCAAAGTAA GCAGCTCCCC
    CAGGTTCCAA GACCCCTGCA GCTCTTTTCA GGTAAGGATC TAAGGGACTC TGGCATTGAC
    ACACACCAGT ACCAAGAAGG ACTCAGTAAG GCCACCCAAG ATCAGATTCT TCAGACTCTC
    AGCCAGAGGG TTCGGAGGCA GAACATTCTC TCATTTGTGC AGCCCTCACA GTTCAACTTT
    ACTCACTCAG GTTTCCCGTT AGAGGACATT TCCACAAGCC AGAGGTTCAT GCTAGGTTTT
    GCTGGCAGAA GGACATCCAA GCCTGCGATG GCAGGTCACT ACTTACTTAA TATTTCCACC
    TATGGCCGGG GTACAGAGAG CTTTAGGAGG ACCCATTCTG TGAACCCCGA AGACCGATTT
    TGTCTGAGTA GCCCCACTGA GGCCTTGAAA ATGGGATACA CAGACTGTAA AAATGCAGCA
    GGAGATAGTA GCAGCGGGAA GGAAGATGAT ACTGACGAGG AAAGTACTGG TGATGAGCAA
    GAATCTGTCC CGGTAAAGGA GGAGCCACAG GCTTCTCAGA GTTCTGGCAA GTGTGATGCA
    AGTTTAGGAC CCCACAGTAG AGAAACCCTG TCCACCAGTG ACTGTTCCGC TAAAAAGAAT
    GTGAAGGCAG AGACACCAGT GCCTGAGCAA ACCCCTTTAA GTAAGGAGAA TTACCTGTTC
    ACTCGAGGCC AAACCTTTGA CGAGAAGACC CTAGCCAGAG ATTTTATTCA GGCAGCACAG
    AAGCAGATGG CTCATGCAGT GAGAGGTAAG ACCATGCGGA GCAGCCCAGA GCTTTTCAAT
    TCTACGTCTC TTCCTCTGCC TGCAGACAGT CCTACCCATC AATCTCTCCT CCTCCCACCA
    CTGCAAACCC CAAAGCTGTA CGGGAGCCCC ACACAGATCG GGCCAAGCTA CAGAGGCATG
    ATCAACGTCT CCACCTCATC AGACATGGAC CATAACTCCT CTGTCCCGGG GATGCCCGAC
    TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT GTCATGTCCT TTTCAGTGAC TGTCACAGCC
    ATCCCTGCCA GCCAGGCCGT GAATGCCGGC AGCCGCGGCC AGACCATTCC TGTTCAGGCC
    TTCCCTGAGG AGAGCAGCGT AGAGGACACT CCTTCCAAGT GTTACTGCCG GTTGAAAGCC
    ATGATCATGT GCAAGGGCTG CGGAGCCTTC TGCCACGACG ATTGCATTGG CCCCTCCAAA
    CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24265
    Clone ID Related Accession (Same CDS sequence) XM_005213080.4 , XM_005193938.3 , XM_005213080.3
    Accession Version XM_005213080.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative Polycomb group protein ASXL2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG TGGAACTTCC CCTCTTGCAT GTCTGAATGC CATGCTGCAC
    ACAAACTCCC GAGGTGAAGA AGGCATCTTC TATAAGGTTC CAGGTAGAAT GGGAGTATAT
    ACTTTGAAGA AAGATGTGCC GGATGGGGTG AAAGAGCTTT CAGAAGGTTC AGAAGAAAGC
    AGTGATGGTC AGTCAGATTC CCAGAGTTCT GAGAACAGCA GCAGCAGCAG TGATGGTGGC
    AGCAACAAGG AGGGAAAAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG GCTGTCACAG
    CCATCTTCTC CTCATTCGGG CTGCCCATCT CCTACCATTC CAGCAGGTAA AGTCATTTCT
    TCATCACAGA AGCACAGCAA GAAGGCACTA AAGCAGGCAC TAAAACAGCA ACAACAGAAG
    AAACAGCAGC AGCAGCAATG CAGGCCAAGC ATGTCCATCT CCTCCAACCA GCACCTCTCT
    CTGAAGACTG TCAAAGCATC CAGTGACCCT GTACCTGCCA AACCTGCGAT ATGGGAAGGA
    AAGCAATCTG ATGGACAGTC AAGCAGCCCT CAGAACTCAA ACTCTAGCTT TTCTTCTTCT
    TCGGTTAAAG TGGAAAATCA TTTGCTGGGC CTGGGGAAGA AGTCATTCCA GAGGTCTGAC
    AGGCTCCACA CAAGGCAAAT GAAGAGAACT AAATGTGCTG AGATTGATGT TGAGACGCCA
    GATTCCATTC TGGTTAATAC AAACCTACGA GCGCTAATCA ACAAACACAC CTTTTCAGTC
    CTTCCTGGAG ACTGCCAACA GCGATTGCTT TTACTACTTC CAGAGGTGGA TCGACAGGTT
    GGTCCAGATG GTCTGATGAA GTTAAATGGC TCAGCCCTTA ATAATGAGTT CTTCACTTCA
    GCAGCCCAAG GCTGGAAGGA AAGACTTTCA GAAGGTGAGT TTACACCGGA GATGCAGGTG
    AGAATTCGAC AAGAGATTGA GAAGGAGAAG AAAGTAGAGC CGTGGAAAGA ACAATTCTTT
    GAAAGCTACT ATGGTCAGAG TTCTGGCCTG AGTCTTGAAG ATTCAAAGAA ATTGACAGCT
    TCACCCAATG ATCCCAAAGT AAAGAAAACC CCAGCTGAGC AACCAAAATC TACGCTTCCT
    TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA GTAATTCCCC AGTTAGAATC TAAAGAGGAA
    GTACTGCAGA TGCCATCACC AGTCAGAAAA GAAGAGCATG AAAGCCAAGA TAAGACGCAG
    CCACACTCCA CAGAGCCCCT TCTTTCCCCA GCTACCAATA CAAATGAGCT TAGCAGCATT
    CCTCCCATCA AGTGCCCGAA GGATGAGGCT CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT
    GAACAGGAAT CTGAGAAAAA GAATCATCTC ACTACAGCTT CAAATTATAA TAAAAATGAA
    AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC AAACCCAAGA GTCCTGGGGT AGAAACAACA
    GTAATGAAGC CCACAGTAGA AGCAAGTCCA CAGGAGACAA CTATGAAAGA GCCTCCATCA
    ACTCTGGCTG ATCACAGCCC AGAAAGCCTC AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG
    GCCCCAACAA GCTGGGAGAA AAGGCCGCGT GTCACTGAGC ATCGCCAGCA CCAGCAGCCA
    TTTCAAGTCT CCCCACAGCC CTTTCTCAGT AGAGGGGACA GGTTCCAGGT GCGGAGAGTA
    CCACCTCTCA AGATCCCGGT CTCCAGAATC TCTCCCATGC CGTTTCCTAC ATCGCAGGTC
    TCTCCCAGGG CTCGTTTTCC AATCTCCATC ACTAGTCCTA ACAGAACAGG AGCCAGAACC
    CTTGCAGACA TCAAAGCAAA AGCCCAGCTT GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC
    GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT GGAGGGACCA TTCCAGGACC TGGCCCAGGC
    GGTGGACAGG GTCCAGGGGA GGGGGGTGAA AGGAGAACTG CTAGAGGAGG GGATCCTGAC
    TCAGACAAAG TCAGTGAGCC CAGCAAGGGC CCCGCACTGG AACTGGCAGG AACTGGAAGC
    AGGGGAGGTA CGAGAGAGCT TTTACCCGCT GGGCCAGACA CTCAGCCCCA GTCTGAGACC
    AGGACCGCAG GCCAGCCACA GCCTCTTAGT GGCCCTGGAG CACAACTACA GCAAACCCCC
    TCAGTGCCTC CCGCATCTGC CGTCGGTGGA GCATGTGCAG GTGTCCCCTC CCCAGCCCAC
    ATTCATGCAC TCCCGCCAGC TTTAGGAAAA TTAAGTAATG AAAAACTAAA TCCCCCCAGG
    GTAACAGGCA CGGTGGCCTC TCTCAGCCAA CCCCAAGGGC CCAGTAATGG TAGGCAGGAG
    AAAGCACCTC CAGCTCCAGC AGATCCTGCT CTGATCATGG GTGCCTCGCC TGTTCATTTT
    GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA GGTTCTAGTA AGAATGCCCC CAATCCTCCG
    GCCTCAGCAG AGATAAGTGC TAGCGCCTCA GTGGATACAA CTGCCTCCCC TTTAACATCT
    CTGTTAACAA CAGCCACTTT AGAGAAGCTC CCTGTGCCGC AGGTCAGCGT AACCACAACG
    CCTACTGGAT CAGCTCCATC CTTGAGCACT TTGCCGGCAG CCTCTAGCCT TAAAACTCCG
    GGAACTTCTT CACATATGAA TGGACCCATT TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT
    AATCCTTTGG TAACTCAGCT GCTGCAGGGC AAAGATGTTC CCATGGAGCA AATTCTGCCT
    AAACCTCTTA CCAAAGTTGA AATGAAAACT GTTCCACTCA CTACAAAAGA GGAGAAGGGA
    GTAGGAGCAC TCATAGGTAC CAGCGTGACA GAGAACAGCA CCAGAGAGGA AGTTCATGAG
    AGACAGTCCC ATCCAGCTGC ACAGCACCTG GGTAAAACTT TGCAAAGTAA GCAGCTCCCC
    CAGGTTCCAA GACCCCTGCA GCTCTTTTCA GGTAAGGATC TAAGGGACTC TGGCATTGAC
    ACACACCAGT ACCAAGAAGG ACTCAGTAAG GCCACCCAAG ATCAGATTCT TCAGACTCTC
    AGCCAGAGGG TTCGGAGGCA GAACATTCTC TCATTTGTGC AGCCCTCACA GTTCAACTTT
    ACTCACTCAG GTTTCCCGTT AGAGGACATT TCCACAAGCC AGAGGTTCAT GCTAGGTTTT
    GCTGGCAGAA GGACATCCAA GCCTGCGATG GCAGGTCACT ACTTACTTAA TATTTCCACC
    TATGGCCGGG GTACAGAGAG CTTTAGGAGG ACCCATTCTG TGAACCCCGA AGACCGATTT
    TGTCTGAGTA GCCCCACTGA GGCCTTGAAA ATGGGATACA CAGACTGTAA AAATGCAGCA
    GGAGATAGTA GCAGCGGGAA GGAAGATGAT ACTGACGAGG AAAGTACTGG TGATGAGCAA
    GAATCTGTCC CGGTAAAGGA GGAGCCACAG GCTTCTCAGA GTTCTGGCAA GTGTGATGCA
    AGTTTAGGAC CCCACAGTAG AGAAACCCTG TCCACCAGTG ACTGTTCCGC TAAAAAGAAT
    GTGAAGGCAG AGACACCAGT GCCTGAGCAA ACCCCTTTAA GTAAGGAGAA TTACCTGTTC
    ACTCGAGGCC AAACCTTTGA CGAGAAGACC CTAGCCAGAG ATTTTATTCA GGCAGCACAG
    AAGCAGATGG CTCATGCAGT GAGAGGTAAG ACCATGCGGA GCAGCCCAGA GCTTTTCAAT
    TCTACGTCTC TTCCTCTGCC TGCAGACAGT CCTACCCATC AATCTCTCCT CCTCCCACCA
    CTGCAAACCC CAAAGCTGTA CGGGAGCCCC ACACAGATCG GGCCAAGCTA CAGAGGCATG
    ATCAACGTCT CCACCTCATC AGACATGGAC CATAACTCCT CTGTCCCGGG GATGCCCGAC
    TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT GTCATGTCCT TTTCAGTGAC TGTCACAGCC
    ATCCCTGCCA GCCAGGCCGT GAATGCCGGC AGCCGCGGCC AGACCATTCC TGTTCAGGCC
    TTCCCTGAGG AGAGCAGCGT AGAGGACACT CCTTCCAAGT GTTACTGCCG GTTGAAAGCC
    ATGATCATGT GCAAGGGCTG CGGAGCCTTC TGCCACGACG ATTGCATTGG CCCCTCCAAA
    CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213137.1
    CDS359..4708
    Translation

    Target ORF information:

    RefSeq Version XM_005213080.3
    Organism Bos taurus(cattle)
    Definition Bos taurus additional sex combs like 2, transcriptional regulator (ASXL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213080.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAGGGAAA AGGGCCGTAG GAAGAAGGGC AGGACCTGGG CGGAGGCCGC CAAGACGGTC 
    TTAGAAAAAT ATCCCAATAC ACCCATGAGT CATAAAGAAA TTCTTCAAGT TATCCAGAGA
    GAAGGACTAA AAGAAATCAG TGGAACTTCC CCTCTTGCAT GTCTGAATGC CATGCTGCAC
    ACAAACTCCC GAGGTGAAGA AGGCATCTTC TATAAGGTTC CAGGTAGAAT GGGAGTATAT
    ACTTTGAAGA AAGATGTGCC GGATGGGGTG AAAGAGCTTT CAGAAGGTTC AGAAGAAAGC
    AGTGATGGTC AGTCAGATTC CCAGAGTTCT GAGAACAGCA GCAGCAGCAG TGATGGTGGC
    AGCAACAAGG AGGGAAAAAA GAGCAGGTGG AAAAGGAAAG TATCATCTAG GCTGTCACAG
    CCATCTTCTC CTCATTCGGG CTGCCCATCT CCTACCATTC CAGCAGGTAA AGTCATTTCT
    TCATCACAGA AGCACAGCAA GAAGGCACTA AAGCAGGCAC TAAAACAGCA ACAACAGAAG
    AAACAGCAGC AGCAGCAATG CAGGCCAAGC ATGTCCATCT CCTCCAACCA GCACCTCTCT
    CTGAAGACTG TCAAAGCATC CAGTGACCCT GTACCTGCCA AACCTGCGAT ATGGGAAGGA
    AAGCAATCTG ATGGACAGTC AAGCAGCCCT CAGAACTCAA ACTCTAGCTT TTCTTCTTCT
    TCGGTTAAAG TGGAAAATCA TTTGCTGGGC CTGGGGAAGA AGTCATTCCA GAGGTCTGAC
    AGGCTCCACA CAAGGCAAAT GAAGAGAACT AAATGTGCTG AGATTGATGT TGAGACGCCA
    GATTCCATTC TGGTTAATAC AAACCTACGA GCGCTAATCA ACAAACACAC CTTTTCAGTC
    CTTCCTGGAG ACTGCCAACA GCGATTGCTT TTACTACTTC CAGAGGTGGA TCGACAGGTT
    GGTCCAGATG GTCTGATGAA GTTAAATGGC TCAGCCCTTA ATAATGAGTT CTTCACTTCA
    GCAGCCCAAG GCTGGAAGGA AAGACTTTCA GAAGGTGAGT TTACACCGGA GATGCAGGTG
    AGAATTCGAC AAGAGATTGA GAAGGAGAAG AAAGTAGAGC CGTGGAAAGA ACAATTCTTT
    GAAAGCTACT ATGGTCAGAG TTCTGGCCTG AGTCTTGAAG ATTCAAAGAA ATTGACAGCT
    TCACCCAATG ATCCCAAAGT AAAGAAAACC CCAGCTGAGC AACCAAAATC TACGCTTCCT
    TCAGAGGCCT CGCCTGTCAG TGTAGTCCCA GTAATTCCCC AGTTAGAATC TAAAGAGGAA
    GTACTGCAGA TGCCATCACC AGTCAGAAAA GAAGAGCATG AAAGCCAAGA TAAGACGCAG
    CCACACTCCA CAGAGCCCCT TCTTTCCCCA GCTACCAATA CAAATGAGCT TAGCAGCATT
    CCTCCCATCA AGTGCCCGAA GGATGAGGCT CTTGTGGAGC AAAAGCCAGT TGCCTCTGCT
    GAACAGGAAT CTGAGAAAAA GAATCATCTC ACTACAGCTT CAAATTATAA TAAAAATGAA
    AGCCAGGAAG CTTTAGTTAC TTCCCTGAGC AAACCCAAGA GTCCTGGGGT AGAAACAACA
    GTAATGAAGC CCACAGTAGA AGCAAGTCCA CAGGAGACAA CTATGAAAGA GCCTCCATCA
    ACTCTGGCTG ATCACAGCCC AGAAAGCCTC AAGAGGAAAT CTTCTCTCAC CCAAGAAGAG
    GCCCCAACAA GCTGGGAGAA AAGGCCGCGT GTCACTGAGC ATCGCCAGCA CCAGCAGCCA
    TTTCAAGTCT CCCCACAGCC CTTTCTCAGT AGAGGGGACA GGTTCCAGGT GCGGAGAGTA
    CCACCTCTCA AGATCCCGGT CTCCAGAATC TCTCCCATGC CGTTTCCTAC ATCGCAGGTC
    TCTCCCAGGG CTCGTTTTCC AATCTCCATC ACTAGTCCTA ACAGAACAGG AGCCAGAACC
    CTTGCAGACA TCAAAGCAAA AGCCCAGCTT GTCAAAGCAC AGAGGGCTGC CGCCGCCGCC
    GCCGCCGCAG CCGCTGCTGC CGCCTCAGTT GGAGGGACCA TTCCAGGACC TGGCCCAGGC
    GGTGGACAGG GTCCAGGGGA GGGGGGTGAA AGGAGAACTG CTAGAGGAGG GGATCCTGAC
    TCAGACAAAG TCAGTGAGCC CAGCAAGGGC CCCGCACTGG AACTGGCAGG AACTGGAAGC
    AGGGGAGGTA CGAGAGAGCT TTTACCCGCT GGGCCAGACA CTCAGCCCCA GTCTGAGACC
    AGGACCGCAG GCCAGCCACA GCCTCTTAGT GGCCCTGGAG CACAACTACA GCAAACCCCC
    TCAGTGCCTC CCGCATCTGC CGTCGGTGGA GCATGTGCAG GTGTCCCCTC CCCAGCCCAC
    ATTCATGCAC TCCCGCCAGC TTTAGGAAAA TTAAGTAATG AAAAACTAAA TCCCCCCAGG
    GTAACAGGCA CGGTGGCCTC TCTCAGCCAA CCCCAAGGGC CCAGTAATGG TAGGCAGGAG
    AAAGCACCTC CAGCTCCAGC AGATCCTGCT CTGATCATGG GTGCCTCGCC TGTTCATTTT
    GCAGCTGCTG GCGCAGTGGA GCCTAAAGCA GGTTCTAGTA AGAATGCCCC CAATCCTCCG
    GCCTCAGCAG AGATAAGTGC TAGCGCCTCA GTGGATACAA CTGCCTCCCC TTTAACATCT
    CTGTTAACAA CAGCCACTTT AGAGAAGCTC CCTGTGCCGC AGGTCAGCGT AACCACAACG
    CCTACTGGAT CAGCTCCATC CTTGAGCACT TTGCCGGCAG CCTCTAGCCT TAAAACTCCG
    GGAACTTCTT CACATATGAA TGGACCCATT TCAAGGCCAA GCTCTAGTAT CCCTGCTAAT
    AATCCTTTGG TAACTCAGCT GCTGCAGGGC AAAGATGTTC CCATGGAGCA AATTCTGCCT
    AAACCTCTTA CCAAAGTTGA AATGAAAACT GTTCCACTCA CTACAAAAGA GGAGAAGGGA
    GTAGGAGCAC TCATAGGTAC CAGCGTGACA GAGAACAGCA CCAGAGAGGA AGTTCATGAG
    AGACAGTCCC ATCCAGCTGC ACAGCACCTG GGTAAAACTT TGCAAAGTAA GCAGCTCCCC
    CAGGTTCCAA GACCCCTGCA GCTCTTTTCA GGTAAGGATC TAAGGGACTC TGGCATTGAC
    ACACACCAGT ACCAAGAAGG ACTCAGTAAG GCCACCCAAG ATCAGATTCT TCAGACTCTC
    AGCCAGAGGG TTCGGAGGCA GAACATTCTC TCATTTGTGC AGCCCTCACA GTTCAACTTT
    ACTCACTCAG GTTTCCCGTT AGAGGACATT TCCACAAGCC AGAGGTTCAT GCTAGGTTTT
    GCTGGCAGAA GGACATCCAA GCCTGCGATG GCAGGTCACT ACTTACTTAA TATTTCCACC
    TATGGCCGGG GTACAGAGAG CTTTAGGAGG ACCCATTCTG TGAACCCCGA AGACCGATTT
    TGTCTGAGTA GCCCCACTGA GGCCTTGAAA ATGGGATACA CAGACTGTAA AAATGCAGCA
    GGAGATAGTA GCAGCGGGAA GGAAGATGAT ACTGACGAGG AAAGTACTGG TGATGAGCAA
    GAATCTGTCC CGGTAAAGGA GGAGCCACAG GCTTCTCAGA GTTCTGGCAA GTGTGATGCA
    AGTTTAGGAC CCCACAGTAG AGAAACCCTG TCCACCAGTG ACTGTTCCGC TAAAAAGAAT
    GTGAAGGCAG AGACACCAGT GCCTGAGCAA ACCCCTTTAA GTAAGGAGAA TTACCTGTTC
    ACTCGAGGCC AAACCTTTGA CGAGAAGACC CTAGCCAGAG ATTTTATTCA GGCAGCACAG
    AAGCAGATGG CTCATGCAGT GAGAGGTAAG ACCATGCGGA GCAGCCCAGA GCTTTTCAAT
    TCTACGTCTC TTCCTCTGCC TGCAGACAGT CCTACCCATC AATCTCTCCT CCTCCCACCA
    CTGCAAACCC CAAAGCTGTA CGGGAGCCCC ACACAGATCG GGCCAAGCTA CAGAGGCATG
    ATCAACGTCT CCACCTCATC AGACATGGAC CATAACTCCT CTGTCCCGGG GATGCCCGAC
    TGTAGTCAGG TGTCTAGCAA TGTTGGTGAT GTCATGTCCT TTTCAGTGAC TGTCACAGCC
    ATCCCTGCCA GCCAGGCCGT GAATGCCGGC AGCCGCGGCC AGACCATTCC TGTTCAGGCC
    TTCCCTGAGG AGAGCAGCGT AGAGGACACT CCTTCCAAGT GTTACTGCCG GTTGAAAGCC
    ATGATCATGT GCAAGGGCTG CGGAGCCTTC TGCCACGACG ATTGCATTGG CCCCTCCAAA
    CTCTGTGTCT CCTGCCTTGT TGTTCGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.