PRKAR2A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKAR2A
Entrez Gene ID	100139910
Full Name	protein kinase cAMP-dependent type II regulatory subunit alpha
General protein information	Preferred Names protein kinase cAMP-dependent type II regulatory subunit alpha Names cAMP-dependent protein kinase type II-alpha regulatory subunit protein kinase, cAMP-dependent, regulatory, type 2, alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191367.1	NP_001178296.1	cAMP-dependent protein kinase type II-alpha regulatory subunit
NM_001191367.2	NP_001178296.1	cAMP-dependent protein kinase type II-alpha regulatory subunit
XM_024982370.1	XP_024838138.1	cAMP-dependent protein kinase type II-alpha regulatory subunit isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PRKAR2A	XP_003432928.1
Bos taurus (cattle)	PRKAR2A	NP_001178296.1
Gallus gallus (chicken)	LOC100858827	XP_003642054.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004940	XP_315029.3
Eremothecium gossypii	AGOS_ADL099C	NP_983997.1
Mus musculus (house mouse)	Prkar2a	NP_032950.1
Rattus norvegicus (Norway rat)	Prkar2a	NP_062137.1
Drosophila melanogaster (fruit fly)	Pka-R2	NP_724860.2
Neurospora crassa	NCU01166	XP_961532.1
Homo sapiens (human)	PRKAR2A	NP_004148.1
Kluyveromyces lactis	KLLA0E04181g	XP_454132.1
Xenopus tropicalis (tropical clawed frog)	prkar2a	XP_002933104.1
Danio rerio (zebrafish)	prkar2aa	NP_001070838.2
Saccharomyces cerevisiae (baker's yeast)	BCY1	NP_012231.1
	LOC708981	XP_002808369.1
Schizosaccharomyces pombe (fission yeast)	cgs1	NP_594274.1
Magnaporthe oryzae (rice blast fungus)	MGG_07335	XP_003715572.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

High phosphorylation of HBV core protein by two alpha-type CK2-activated cAMP-dependent protein kinases in vitro.
Enomoto M, Sawano Y, Kosuge S, Yamano Y, Kuroki K, Ohtsuki K
FEBS letters580(3)894-9(2006 Feb)

Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regions.
Vigil D, Blumenthal DK, Heller WT, Brown S, Canaves JM, Taylor SS, Trewhella J
Journal of molecular biology337(5)1183-94(2004 Apr)

Predicted structures of cAMP binding domains of type I and II regulatory subunits of cAMP-dependent protein kinase.
Weber IT, Steitz TA, Bubis J, Taylor SS
Biochemistry26(2)343-51(1987 Jan)

Primary structure of the regulatory subunit of type II cAMP-dependent protein kinase from bovine cardiac muscle.
Takio K, Smith SB, Krebs EG, Walsh KA, Titani K
Proceedings of the National Academy of Sciences of the United States of America79(8)2544-8(1982 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

angle X-ray scattering studies indicate RIalpha, RIIalpha, and RIIbeta homodimers differ markedly in overall shape despite extensive sequence homology and similar molecular masses;cAMP binding does not cause large conformational changes(Prkar1a, Prkar2a)
Title: Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regions.

The following PRKAR2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAR2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36891	NM_001191367.1 Latest version!	Bos taurus protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa216327	NM_001191367.2 Latest version!	Bos taurus protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa228716	XM_024982370.1 Latest version!	Bos taurus protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAR2A ( NM_001191367.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178296.1 Bos taurus protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A), mRNA.

Price & Availability↑

CloneID	OBa36891
Clone ID Related Accession (Same CDS sequence)	NM_001191367.1
Accession Version	NM_001191367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	cAMP-dependent protein kinase type II-alpha regulatory subunit
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03037540.1, AAFC03037548.1 and AAFC02092910.1. On Jul 17, 2010 this sequence version replaced XM_001790285.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCACA TTCAGATCCC GCCCGGGCTC ACGGAGCTGC TGCAGGGCTA CACCGTGGAG 
GTGCTGCGGC AGCGGCCACC AGACCTCGTA GACTTCGCGG TGGACTATTT CACCCGCCTG 
CGAGAGGCCC GCTCCAGAGC CTCCACCCCG CCCGCCGCCC CTCCTTCCGG CTCCCAGGAT 
TTCGATCCCG GCGCTGGCCT GGTCGCCGAC GCGGTGGCGG ACAGCGAGTC GGAGGACGAA 
GAGGACTTGG ACGTTCCAAT TCCTGGCAGA TTTGATCGGC GAGTGTCAGT CTGTGCTGAG 
ACCTATAACC CTGATGAGGA AGAAGAAGAT ACTGATCCGA GGGTGATTCA TCCTAAAACT 
GATCAACAGC GGTGCAGACT TCAGGAAGCT TGCAAAGATA TTCTTCTTTT CAAAAATCTT 
GATCCGGAAC AGCTATCCCA AGTCCTTGAT GCCATGTTTG AAAGGACGGT CAAAGTTGAT 
GAGCATGTCA TTGACCAAGG AGATGATGGA GACAACTTTT ATGTCATTGA ACGGGGAACC 
TATGATATTT TAGTAACAAA AGATAATCAA ACCCGCTCTG TTGGTCAGTA TGACAACCAT 
GGAAGTTTTG GAGAACTAGC TCTGATGTAC AACACCCCAA GAGCTGCTAC CATTGTTGCC 
ACTTCAGAAG GCTCCCTTTG GGGACTGGAC CGTGTGACAT TTAGAAGAAT CATAGTGAAA 
AACAATGCAA AAAAGAGGAA GATGTTTGAA TCATTTATTG AATCTGTGCC GCTTCTTAAA 
TCACTAGAGG TGTCAGAACG AATGAAGATC GTGGACGTAA TAGGAGAAAA GGTCTATAAG 
GATGGAGAGC GCATAATCAC TCAGGGTGAA AAGGCTGATA GCTTTTACAT TATAGAGTCT 
GGTGAAGTGA GCATCTTGAT CAAAAGCAAG ACTAAAGTCA ACAAGGATGG TGAGAACCAG 
GAGGTCGAGA TTGCGCGCTG CCACAAGGGG CAGTACTTTG GAGAGCTTGC ACTGGTCACC 
AACAAACCCA GAGCTGCTTC GGCTTATGCA GTGGGAGATG TGAAATGCTT AGTTATGGAT 
GTGCAAGCAT TCGAGAGGCT GCTGGGGCCC TGCATGGACA TCATGAAGAG GAACATCTCG 
CACTATGAGG AACAGCTGGT GAAGATGTTT GGCTCCAGCA TGGATCTGAT CGATCCTGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178296.1
CDS	295..1500
Translation	MSHIQIPPGLTELLQGYTVEVLRQRPPDLVDFAVDYFTRLREARSRASTPPAAPPSGSQDFDPGAGLVADAVADSESEDEEDLDVPIPGRFDRRVSVCAETYNPDEEEEDTDPRVIHPKTDQQRCRLQEACKDILLFKNLDPEQLSQVLDAMFERTVKVDEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNHGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKVYKDGERIITQGEKADSFYIIESGEVSILIKSKTKVNKDGENQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSMDLIDPGQ

Target ORF information:

RefSeq Version	NM_001191367.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191367.1

ORF Insert Sequence:

ATGAGCCACA TTCAGATCCC GCCCGGGCTC ACGGAGCTGC TGCAGGGCTA CACCGTGGAG 
GTGCTGCGGC AGCGGCCACC AGACCTCGTA GACTTCGCGG TGGACTATTT CACCCGCCTG 
CGAGAGGCCC GCTCCAGAGC CTCCACCCCG CCCGCCGCCC CTCCTTCCGG CTCCCAGGAT 
TTCGATCCCG GCGCTGGCCT GGTCGCCGAC GCGGTGGCGG ACAGCGAGTC GGAGGACGAA 
GAGGACTTGG ACGTTCCAAT TCCTGGCAGA TTTGATCGGC GAGTGTCAGT CTGTGCTGAG 
ACCTATAACC CTGATGAGGA AGAAGAAGAT ACTGATCCGA GGGTGATTCA TCCTAAAACT 
GATCAACAGC GGTGCAGACT TCAGGAAGCT TGCAAAGATA TTCTTCTTTT CAAAAATCTT 
GATCCGGAAC AGCTATCCCA AGTCCTTGAT GCCATGTTTG AAAGGACGGT CAAAGTTGAT 
GAGCATGTCA TTGACCAAGG AGATGATGGA GACAACTTTT ATGTCATTGA ACGGGGAACC 
TATGATATTT TAGTAACAAA AGATAATCAA ACCCGCTCTG TTGGTCAGTA TGACAACCAT 
GGAAGTTTTG GAGAACTAGC TCTGATGTAC AACACCCCAA GAGCTGCTAC CATTGTTGCC 
ACTTCAGAAG GCTCCCTTTG GGGACTGGAC CGTGTGACAT TTAGAAGAAT CATAGTGAAA 
AACAATGCAA AAAAGAGGAA GATGTTTGAA TCATTTATTG AATCTGTGCC GCTTCTTAAA 
TCACTAGAGG TGTCAGAACG AATGAAGATC GTGGACGTAA TAGGAGAAAA GGTCTATAAG 
GATGGAGAGC GCATAATCAC TCAGGGTGAA AAGGCTGATA GCTTTTACAT TATAGAGTCT 
GGTGAAGTGA GCATCTTGAT CAAAAGCAAG ACTAAAGTCA ACAAGGATGG TGAGAACCAG 
GAGGTCGAGA TTGCGCGCTG CCACAAGGGG CAGTACTTTG GAGAGCTTGC ACTGGTCACC 
AACAAACCCA GAGCTGCTTC GGCTTATGCA GTGGGAGATG TGAAATGCTT AGTTATGGAT 
GTGCAAGCAT TCGAGAGGCT GCTGGGGCCC TGCATGGACA TCATGAAGAG GAACATCTCG 
CACTATGAGG AACAGCTGGT GAAGATGTTT GGCTCCAGCA TGGATCTGAT CGATCCTGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAR2A ( NM_001191367.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178296.1 Bos taurus protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), mRNA.

Price & Availability↑

CloneID	OBa216327
Clone ID Related Accession (Same CDS sequence)	NM_001191367.2
Accession Version	NM_001191367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Bos taurus(cattle)
Product	cAMP-dependent protein kinase type II-alpha regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000022.1. On Jun 30, 2017 this sequence version replaced NM_001191367.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5765710.2642.1, SRR6764281.16347.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCACA TTCAGATCCC GCCCGGGCTC ACGGAGCTGC TGCAGGGCTA CACCGTGGAG 
GTGCTGCGGC AGCGGCCACC AGACCTCGTA GACTTCGCGG TGGACTATTT CACCCGCCTG 
CGAGAGGCCC GCTCCAGAGC CTCCACCCCG CCCGCCGCCC CTCCTTCCGG CTCCCAGGAT 
TTCGATCCCG GCGCTGGCCT GGTCGCCGAC GCGGTGGCGG ACAGCGAGTC GGAGGACGAA 
GAGGACTTGG ACGTTCCAAT TCCTGGCAGA TTTGATCGGC GAGTGTCAGT CTGTGCTGAG 
ACCTATAACC CTGATGAGGA AGAAGAAGAT ACTGATCCGA GGGTGATTCA TCCTAAAACT 
GATCAACAGC GGTGCAGACT TCAGGAAGCT TGCAAAGATA TTCTTCTTTT CAAAAATCTT 
GATCCGGAAC AGCTATCCCA AGTCCTTGAT GCCATGTTTG AAAGGACGGT CAAAGTTGAT 
GAGCATGTCA TTGACCAAGG AGATGATGGA GACAACTTTT ATGTCATTGA ACGGGGAACC 
TATGATATTT TAGTAACAAA AGATAATCAA ACCCGCTCTG TTGGTCAGTA TGACAACCAT 
GGAAGTTTTG GAGAACTAGC TCTGATGTAC AACACCCCAA GAGCTGCTAC CATTGTTGCC 
ACTTCAGAAG GCTCCCTTTG GGGACTGGAC CGTGTGACAT TTAGAAGAAT CATAGTGAAA 
AACAATGCAA AAAAGAGGAA GATGTTTGAA TCATTTATTG AATCTGTGCC GCTTCTTAAA 
TCACTAGAGG TGTCAGAGCG AATGAAGATC GTGGACGTAA TAGGAGAAAA GGTCTATAAG 
GATGGAGAGC GCATAATCAC TCAGGGTGAA AAGGCTGATA GCTTTTACAT TATAGAGTCT 
GGTGAAGTGA GCATCTTGAT CAAAAGCAAG ACTAAAGTCA ACAAGGATGG TGAGAACCAG 
GAGGTCGAGA TTGCGCGCTG CCACAAGGGG CAGTACTTTG GAGAGCTTGC ACTGGTCACC 
AACAAACCCA GAGCTGCTTC GGCTTATGCA GTGGGAGATG TGAAATGCTT AGTTATGGAT 
GTGCAAGCAT TCGAGAGGCT GCTGGGGCCC TGCATGGACA TCATGAAGAG GAACATCTCG 
CACTATGAGG AACAGCTGGT GAAGATGTTT GGCTCCAGCA TGGATCTGAT CGATCCTGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178296.1
CDS	295..1500
Translation	MSHIQIPPGLTELLQGYTVEVLRQRPPDLVDFAVDYFTRLREARSRASTPPAAPPSGSQDFDPGAGLVADAVADSESEDEEDLDVPIPGRFDRRVSVCAETYNPDEEEEDTDPRVIHPKTDQQRCRLQEACKDILLFKNLDPEQLSQVLDAMFERTVKVDEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNHGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKVYKDGERIITQGEKADSFYIIESGEVSILIKSKTKVNKDGENQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSMDLIDPGQ

Target ORF information:

RefSeq Version	NM_001191367.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191367.2

ORF Insert Sequence:

ATGAGCCACA TTCAGATCCC GCCCGGGCTC ACGGAGCTGC TGCAGGGCTA CACCGTGGAG 
GTGCTGCGGC AGCGGCCACC AGACCTCGTA GACTTCGCGG TGGACTATTT CACCCGCCTG 
CGAGAGGCCC GCTCCAGAGC CTCCACCCCG CCCGCCGCCC CTCCTTCCGG CTCCCAGGAT 
TTCGATCCCG GCGCTGGCCT GGTCGCCGAC GCGGTGGCGG ACAGCGAGTC GGAGGACGAA 
GAGGACTTGG ACGTTCCAAT TCCTGGCAGA TTTGATCGGC GAGTGTCAGT CTGTGCTGAG 
ACCTATAACC CTGATGAGGA AGAAGAAGAT ACTGATCCGA GGGTGATTCA TCCTAAAACT 
GATCAACAGC GGTGCAGACT TCAGGAAGCT TGCAAAGATA TTCTTCTTTT CAAAAATCTT 
GATCCGGAAC AGCTATCCCA AGTCCTTGAT GCCATGTTTG AAAGGACGGT CAAAGTTGAT 
GAGCATGTCA TTGACCAAGG AGATGATGGA GACAACTTTT ATGTCATTGA ACGGGGAACC 
TATGATATTT TAGTAACAAA AGATAATCAA ACCCGCTCTG TTGGTCAGTA TGACAACCAT 
GGAAGTTTTG GAGAACTAGC TCTGATGTAC AACACCCCAA GAGCTGCTAC CATTGTTGCC 
ACTTCAGAAG GCTCCCTTTG GGGACTGGAC CGTGTGACAT TTAGAAGAAT CATAGTGAAA 
AACAATGCAA AAAAGAGGAA GATGTTTGAA TCATTTATTG AATCTGTGCC GCTTCTTAAA 
TCACTAGAGG TGTCAGAGCG AATGAAGATC GTGGACGTAA TAGGAGAAAA GGTCTATAAG 
GATGGAGAGC GCATAATCAC TCAGGGTGAA AAGGCTGATA GCTTTTACAT TATAGAGTCT 
GGTGAAGTGA GCATCTTGAT CAAAAGCAAG ACTAAAGTCA ACAAGGATGG TGAGAACCAG 
GAGGTCGAGA TTGCGCGCTG CCACAAGGGG CAGTACTTTG GAGAGCTTGC ACTGGTCACC 
AACAAACCCA GAGCTGCTTC GGCTTATGCA GTGGGAGATG TGAAATGCTT AGTTATGGAT 
GTGCAAGCAT TCGAGAGGCT GCTGGGGCCC TGCATGGACA TCATGAAGAG GAACATCTCG 
CACTATGAGG AACAGCTGGT GAAGATGTTT GGCTCCAGCA TGGATCTGAT CGATCCTGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAR2A ( XM_024982370.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838138.1 Bos taurus protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228716
Clone ID Related Accession (Same CDS sequence)	XM_024982370.1
Accession Version	XM_024982370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cAMP-dependent protein kinase type II-alpha regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGG GAGTAAAGAA GGAAAGAAGA CAGAGCCTGC AGAAGATTCA GATAAAAAAT 
GTTCCAATTC CTGGCAGATT TGATCGGCGA GTGTCAGTCT GTGCTGAGAC CTATAACCCT 
GATGAGGAAG AAGAAGATAC TGATCCGAGG GTGATTCATC CTAAAACTGA TCAACAGCGG 
TGCAGACTTC AGGAAGCTTG CAAAGATATT CTTCTTTTCA AAAATCTTGA TCCGGAACAG 
CTATCCCAAG TCCTTGATGC CATGTTTGAA AGGACGGTCA AAGTTGATGA GCATGTCATT 
GACCAAGGAG ATGATGGAGA CAACTTTTAT GTCATTGAAC GGGGAACCTA TGATATTTTA 
GTAACAAAAG ATAATCAAAC CCGCTCTGTT GGTCAGTATG ACAACCATGG AAGTTTTGGA 
GAACTAGCTC TGATGTACAA CACCCCAAGA GCTGCTACCA TTGTTGCCAC TTCAGAAGGC 
TCCCTTTGGG GACTGGACCG TGTGACATTT AGAAGAATCA TAGTGAAAAA CAATGCAAAA 
AAGAGGAAGA TGTTTGAATC ATTTATTGAA TCTGTGCCGC TTCTTAAATC ACTAGAGGTG 
TCAGAGCGAA TGAAGATCGT GGACGTAATA GGAGAAAAGG TCTATAAGGA TGGAGAGCGC 
ATAATCACTC AGGGTGAAAA GGCTGATAGC TTTTACATTA TAGAGTCTGG TGAAGTGAGC 
ATCTTGATCA AAAGCAAGAC TAAAGTCAAC AAGGATGGTG AGAACCAGGA GGTCGAGATT 
GCGCGCTGCC ACAAGGGGCA GTACTTTGGA GAGCTTGCAC TGGTCACCAA CAAACCCAGA 
GCTGCTTCGG CTTATGCAGT GGGAGATGTG AAATGCTTAG TTATGGATGT GCAAGCATTC 
GAGAGGCTGC TGGGGCCCTG CATGGACATC ATGAAGAGGA ACATCTCGCA CTATGAGGAA 
CAGCTGGTGA AGATGTTTGG CTCCAGCATG GATCTGATCG ATCCTGGGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838138.1
CDS	16..1029
Translation	MKEGVKKERRQSLQKIQIKNVPIPGRFDRRVSVCAETYNPDEEEEDTDPRVIHPKTDQQRCRLQEACKDILLFKNLDPEQLSQVLDAMFERTVKVDEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNHGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKVYKDGERIITQGEKADSFYIIESGEVSILIKSKTKVNKDGENQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSMDLIDPGQ

Target ORF information:

RefSeq Version	XM_024982370.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cAMP-dependent type II regulatory subunit alpha (PRKAR2A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982370.1

ORF Insert Sequence:

ATGAAGGAGG GAGTAAAGAA GGAAAGAAGA CAGAGCCTGC AGAAGATTCA GATAAAAAAT 
GTTCCAATTC CTGGCAGATT TGATCGGCGA GTGTCAGTCT GTGCTGAGAC CTATAACCCT 
GATGAGGAAG AAGAAGATAC TGATCCGAGG GTGATTCATC CTAAAACTGA TCAACAGCGG 
TGCAGACTTC AGGAAGCTTG CAAAGATATT CTTCTTTTCA AAAATCTTGA TCCGGAACAG 
CTATCCCAAG TCCTTGATGC CATGTTTGAA AGGACGGTCA AAGTTGATGA GCATGTCATT 
GACCAAGGAG ATGATGGAGA CAACTTTTAT GTCATTGAAC GGGGAACCTA TGATATTTTA 
GTAACAAAAG ATAATCAAAC CCGCTCTGTT GGTCAGTATG ACAACCATGG AAGTTTTGGA 
GAACTAGCTC TGATGTACAA CACCCCAAGA GCTGCTACCA TTGTTGCCAC TTCAGAAGGC 
TCCCTTTGGG GACTGGACCG TGTGACATTT AGAAGAATCA TAGTGAAAAA CAATGCAAAA 
AAGAGGAAGA TGTTTGAATC ATTTATTGAA TCTGTGCCGC TTCTTAAATC ACTAGAGGTG 
TCAGAGCGAA TGAAGATCGT GGACGTAATA GGAGAAAAGG TCTATAAGGA TGGAGAGCGC 
ATAATCACTC AGGGTGAAAA GGCTGATAGC TTTTACATTA TAGAGTCTGG TGAAGTGAGC 
ATCTTGATCA AAAGCAAGAC TAAAGTCAAC AAGGATGGTG AGAACCAGGA GGTCGAGATT 
GCGCGCTGCC ACAAGGGGCA GTACTTTGGA GAGCTTGCAC TGGTCACCAA CAAACCCAGA 
GCTGCTTCGG CTTATGCAGT GGGAGATGTG AAATGCTTAG TTATGGATGT GCAAGCATTC 
GAGAGGCTGC TGGGGCCCTG CATGGACATC ATGAAGAGGA ACATCTCGCA CTATGAGGAA 
CAGCTGGTGA AGATGTTTGG CTCCAGCATG GATCTGATCG ATCCTGGGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

High phosphorylation of HBV core protein by two alpha-type CK2-activated cAMP-dependent protein kinases in vitro.
FEBS letters580(3)894-9(2006 Feb)
Enomoto M,Sawano Y,Kosuge S,Yamano Y,Kuroki K,Ohtsuki K

Conformational differences among solution structures of the type Ialpha, IIalpha and IIbeta protein kinase A regulatory subunit homodimers: role of the linker regions.
Journal of molecular biology337(5)1183-94(2004 Apr)
Vigil D,Blumenthal DK,Heller WT,Brown S,Canaves JM,Taylor SS,Trewhella J

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