SHROOM3 cDNA ORF clone, Bos taurus(cattle)

The following SHROOM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHROOM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235082 XM_015471673.2
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235083 XM_010806187.3
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235084 XM_024992949.1
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235084 XM_010806188.3
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235084 XM_010806190.3
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235084 XM_010806189.3
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235081 XM_024992948.1
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa38287 NM_001191524.1
Latest version!
Bos taurus shroom family member 3 (SHROOM3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa58951 XM_010806187.2
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa58952 XM_010806190.2
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa58952 XM_010806189.2
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa58952 XM_010806188.2
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa90945 XM_015471673.1
Latest version!
Bos taurus shroom family member 3 (SHROOM3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235082
    Clone ID Related Accession (Same CDS sequence) XM_015471673.2
    Accession Version XM_015471673.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTGCTAA GCAAGGGCGG CTGCCTGGCT CTGATGTTCC CCAGGCTTGG GACCCAAAAC 
    TCGAGAAGGC GGCAAAATCC AGATGCACAT GCAGACCCGG GCCCTGCAGA CACTGGCACC
    TCTGACTCAT TCGGCCCAGA GCACCTCACC TCTGACCCCC AGCACAGAAA AGTAGCATGG
    TCAGGAGGGG TTAAACCTCG GCTGAAGCAC AGACGCACTG AACCTGCAGG CCGGCCTCAT
    TCTTGGCACT CGACCAAACT TGGGGAGAAC CAACCCGATG CCAGCATGAT GCAGATATCT
    CAGGGCACGA TTGGCACGCC TTGGCCCCAG AGCTACCATT CCAGCTCCTC CACCAGTGAC
    CTCTCCGGCT ATGACCACGC ATATCTGAGG CAGAGCCCTG ACCGGTGCAG CTCCCAGGGG
    AGCATGGAGA GCCTGGAGGC CGGCGGGGGA TACCCACCCT GCCACCTTCT CTCCCCGGCC
    AAGTCCACCA GCAGCATTGA CCAGCTCAGC CACCTCCACA GCAAGAGAGA TTCGGCTTAC
    AGCTCCTTCT CCACCAGTTC CAGCATCCTC GAGTATCCAC CCCCTGGCGT CTCTGGCCGG
    GAGCGTTCGG GCTCCATGGA CACCACTTCT GCTCGAGGTG GCCTCCTAGA AGGGATGAAG
    CAGGCAGATA TTCGCTATAT TAAGACAGTC TATGACCCCC GGAGGGGCGT CTCAGCAGAG
    TATGAGGTGA ACTCTTCAGG CCTGCTTCTC CAAGGTAGGG AGACCCGAGC CTCAACTGAT
    GGTCAGGGCT ACAATAAATT CCATAATGTT CCTCGGGGCA AAGGCGCACC ACCCTCTTCC
    TGGAGCCAGC AGTGCCCTGG TTCCTTAGAG ACCACTGTGG ACAACTTGCC TCCTAAAGTG
    GGTGCGCCCC TGCCCCCAGC TCGGAGTGAC AGTTATGCAG CCTTCCGGCA GCGAGAGAGG
    CCCAGCTCCT GGTCTAGCCT AGATCAGAAA CGGTTCTGCC GACCTCCAGC AAACTCTGCA
    GGCACCCTGA AATCTCCCTT CATAGAGGAA CAGCTGCATA CTGTGCTAGA GAAGAGCCCA
    GAAAACAGCC CTCCAGTGAA GCCCAAGCAC AATTATACCC AGAAGGCCCA GCCCGGCCAA
    CCTCTGCTGC CGACTGGCAT CTACCCGGTA CCTTCCCTGG AGCCACACTT CGCCCAGGTG
    CCCCGGCCTT CTGTGAGTGG AAATGGTACC CTCTACCCTG CCCTGGCCAA GGAGGGTGGG
    TACACACCTC CTCAGGGAGC GTGCGACAGA ATGGCTGCCC TTGATGAGAA TGGGAACCAA
    AACGGATCTA GCAGGCCTGG CTTCGCCTTC TGCCAGGCCT TAGAACGGGA CTCAGTGTCC
    CTAGTAGAGA GGAAACCTGA AACTTCAGCC AAATGTGTCC CCTATAAAGT CCATTTTCCC
    TCAGTGCCTG AGAACGAGGA GGAGACCTCC CTGAAGAGAC TTCTCACACC TCTAGAAGGC
    CACAGCCCAC ATCCCAGTGA GAGAAAGAGC ACCCAGGGCA ACAGATATTC TAATTACCAC
    AGCCTCCAAT CCCCTCAGGC TCAGGTGTGC GAAGTGGGTG AAGACAAGAG ATCCTTCCTG
    CCTTCAGAGC CTTGGGAGGG GGATTCCCAT GAAGACCACA ATGCCAACCT CCGGGGGAGG
    CTCCACGGAG GCAGCCTGGG CCAGAGTATG CCAGGCAGCT TTGGCAAGAC TGCAGCTGCC
    TTCTCCTCCC TCCAGAACAT TCCTGAGAGT CTAAGAAGGC AAAGCAGCTT GGATCTCGGA
    GCAGCAGCCC AGGAAGTCTA CCTGGAAGGC TCATCCACCT GCGCAGTCAA TACCAAGGGA
    GAAGAGTCTG GAAGGACTGC TGTTGCTGAT CACAAGAGCC AGCTGGACAG GTTGGTGTCC
    TATCCAAGGC CCGAGGGGAG AACCTTGGCC TCTTTCCAGA GTTCAGACCC AACGTATGAA
    GAGCCGCCCT CCCCATCCCC ACAGGAGACC TCCAGTCTGG GCCGGAGGAG GCTGAGTTCC
    AGCAGCACCT CGGCTCTGCA GGGCTTTCAG TACGGGAAGC CCCACTGCTC CGTGCTGGAG
    AAGGTTTCCA AGATCGAGCA GCGAGAGCAG GCTGGCCAGA GACCCCCGAG TGCCGGCGGC
    TCTACTTATG GCTATAATTA CAGGCCCCAC AGGACACTCC CAGCCTCCAA TACTTTGGGG
    AATGACTCGG AGGAGACTAA AGGCCGTATA CATTTTTCCG AACTCACTGA ACCCCTAGGC
    AATGGAGAAC AGCACTTCAA AAACGGGGAG CCAAAGTCAG AAGAGGTTTC CTGGCAGCCG
    TGTGGTCAAC AGCCGAGGCG GGCTGTGGAG GGTGGCCGGG GTGCCCCACT CCGGGGTGGA
    GAGCCCCCGA GGCGGGACGC CCGTCTGCTC CGCAGCCAGA GCAGCTTCCA GCTCTTCAGC
    GAGGCCGAGA AGGAGGCCAT GTGGTCAGAC GACAGGCCCC GCACGCCAGA GTCGACTGTG
    CAGGAGGCCC CCTTCAGCCG CGCCTACCGG AACAGCATCA AGGATGCACA GTCCCGCGTC
    CTGGGAGCCA CGTCCTTCCG ACGCCGGGAC CTCGAGCCTG GGACGTCTGT GGCCTCCAGG
    CCCTGGCGCC TGCGACCCGC CTCGGCCCAC GTAGGGCTGC GCAGCCCGGA GGCACCGATT
    TCCGCCTCCC CGCACACCCC GCGCGAGCGG CACAGCGTGA CCCCGGCCGA GGGGGACCCT
    GCCCGCCTCG CGCCCCCTGC TGCCCGGAGG GGCCCGCGCC GGCATCTGAC CACCGAGCAG
    AAGAAGCGCT CGTACTCAGA GCCCGAGAAG ATGCACGAGG TGGGGGTCTC GGAGGAACCC
    GAGCCCGAGC CCCCTGGGCC GCCGAGGAAG GGGCCGCATT TCATGGAGGG CTCAGTGACT
    GACCGGCGCC GCATCTTCGA GCGAGACGGC AGGGCCTGCT CTACCCTCAG CCTGTCGGGG
    CCTGAGCTGA AGCAGTTCCA GCAGAGCGCA CTGGCCGACT ACATTCAGCG CAAGACCGGC
    AAGCGGCCTG CGGGCGCCGG CTGTGGCCTG CAGGAGCCGG GGCGGCTGCT GGAGCGCACC
    CAGAGCACCT ACCTCCAGCC CGAGGGCCCG GGCCTCGCCG CGGCTTCCAG TCTCTGCTCG
    CTTCGGGAGC CCAGCCTGCC GCCCCGCAGG GAGACCGCCC TTCTGCCAGC CACTGTGGTG
    GGGAGTACTG GGGAGACCCA GCGGGGCCCC CGAGATCGCA GCAGCTCGTT TGCCAGCGGC
    CGCCACCTTG GGGAAAGGCA CCGCGGGGAC TCAGCGCCGA GAGAACTGCT CAGTGGAGCT
    AACAATGGAT CAAAGGGCCC CCAGAGACTG AACGGTACCC CAGGGGAACC CTCCCCGTGG
    GGGGCCACAG CTGGGAGGGC CGCGAAGTCC ATGTCGGCTG AGGACCTGCT GGAGCGCTCA
    GACGTCCAGG CTGTCCCTGT CCACATGAGG TCCCGGTCAT CTCCCACCGC CGACAAGCGC
    CAGGATGTGC TTTTGGGGGA AAACAGTAGC TTTGGTCTCA TGAAGGATCC GTGTTATTTG
    ACTGGCCCAG GTTCCAGGCC ATTCAGCTGT TCCAGCCGTG AAGAGATGCC AGCGCTCAGG
    CACCACCCCA GCCCTCACTG GGGGGATTCG GGCTGCAAAG CAGGTAGTGG TGGTGCCTCT
    GTTCCCAGCG GGGGTCCGGG GCCGCTGGAC CCTCCGAGAC AGGCCAGCAG AACGCCTTGC
    CCTCTGCCAC TGTCCTCGGG AGCTCATGGG CATCTTCCAG ACGCCAGGGC TGCACCCCTG
    AGCTCGGTCT TGTCTGCCCC TGGCCCTTCG AGCTATTGCT CACAGGCTGC AGCCCAGCCC
    TCCACACCAG CCGGCACCCC CAGAAGCGGC AGTTGCCACA GCCCGGCCCA GCCTCCCAGC
    CCTGAGCGCA AGACAGAAGA GCTCGCAGGG ATGCGGGCCT CCCCGCCCTG GATGAAGCGG
    ACCTACATTG TCAGAGAGGA GAGCCTCCCC GAGGACCCCC CGAAGCACTA CCCCAAGCCA
    CCGGATGCCC CCAGCTCAAG CAGCACTTCT GACCCGGATA CGCCTCTCGG GGCCCCAGGC
    ATTCTGGGCA GGATTTCCCT CCGGATCTCC GAGTCGGCCT TGCTGGCTTC CCCGCCACCT
    CGCGAGGACT GCGAGGACGA CGAGGTGTTT GTGAGGGACC CACGCCCCAC CGCCACTTCC
    AGCCCCCGCT GCGATGAGCT GCTGCTGCCC CCTCCGCCTC CGCCCCCACC ACCAACAACC
    AGCCAGGCAT CCCTGGCCCA GGGCCTGGAC CACTTCCCTC CGCCTCCTCC CGAAGCTATG
    TGCCCGGCCC AGTGGGAGGA GGGCTACCTG GAGCCCCGTG CCAGCTCCAG CAAACTTGCC
    AAGGTGACAG TTGCCAAGGA AAGGTCCATG GCCGGTGCAG CCCATCCGGT TGATAATCAG
    CTGCTGGGTT CCAGATCGCA AACTTCAGGC AAGAGTTTCG AGGCCAAGGA GACCAACCCA
    CCCTCCACCA CGGGTGCTCT GCCCCAGCCT GCTGGGTCTC TAAGCAAGCA GCCAAGCCCA
    GGACAGCCAC CTCCCATGCA GACCCACAGC CTCATCCACG AGCCAGCCAT TGGGACTGCA
    GGTTTAGAAA AGAATGTCAG TTCTGGTCCT CAAAAGACCT CAGAAGACAT CAGAACAGAG
    ACGCTAGCCA AGGAAATTAT CCACCAAGAC AAATCTCTGG CAGACATTTT GGACCCAGAC
    TCCAGAATGA AGACAACTAT GGACCTGATG GAAGGTTTGT TTCCTCGAGA TGTTAATTTG
    CTGAAGGAAA ACAGCGTAAA GAAGAAGGCC GTGCAGAAAA CCATCAGCTT CCCAGGATGT
    GAGACCAAGA GGAGTGACGA CAAGGAAGCA GTGGGCATGT TGGTCAACTG TCCTGCCTAC
    TACAGCGTCT CTGCTCCCAA GGCTGAGCTT CTCAACAAAA TCAAAGCTAT GCCAGAAGAG
    GTGAACGAGG AAGAGGAGCA GGCAGACATC AACGAAAAGA AGGCGGAGCT GATCGGAAGC
    CTCACCCACA AGCTCGAGAC CCTCCAGGAA GCCAAGGGGA GCCTGCTGAT GGACATCAAG
    CTCAATAACG CCCTGGGGGA GGAGGTGGAG GCCTGGATCA GTGAGCTCTG CAAGCCCAAC
    GAGATCGACA AGTACAAGAT GTTCATCGGG GACTTGGACA AGGTGGTGAA CCTGCTCCTG
    TCCCTCTCGG GGCGCCTGGC CCGCGTGGAG AACGTGCTCA GCGGCCTTGG TGAAGACGCC
    AGTAACGAGG AAAGGAGTTC ACTCAACGAG AAGAGGAAGG TCCTGTCTGC TCAGCACGAG
    GACGCCCGGG AGCTCAAGGA GAACGTGGAT CGGAGGGAGC GCGTGGTGCT GGACATCCTG
    GCCAATTACC TCTCCGAAGA GCAGCTCCAA GATTACCGGC ATTTCGTGAA AATGAAGTCG
    ACACTCCTCA TCGAGCAGCG AGAGCTGGAT GACAAGATCA AGCTGGGCCA GGAGCAGGTC
    AAGTGCCTGC TAGAGAGCCT TCCCTCGGAC TTCGTGCCGA AGGCGGGGGC CCTGGCTCTG
    CCCCCAGGCC TCACCGGCGA TGTGACTCCC GTGGGGGGCT GGACTGGCAG TGGTGTTTTC
    CCAGCGGTGA CCTCCCCACT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327159.2
    CDS68..5791
    Translation

    Target ORF information:

    RefSeq Version XM_015471673.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGTGCTAA GCAAGGGCGG CTGCCTGGCT CTGATGTTCC CCAGGCTTGG GACCCAAAAC 
    TCGAGAAGGC GGCAAAATCC AGATGCACAT GCAGACCCGG GCCCTGCAGA CACTGGCACC
    TCTGACTCAT TCGGCCCAGA GCACCTCACC TCTGACCCCC AGCACAGAAA AGTAGCATGG
    TCAGGAGGGG TTAAACCTCG GCTGAAGCAC AGACGCACTG AACCTGCAGG CCGGCCTCAT
    TCTTGGCACT CGACCAAACT TGGGGAGAAC CAACCCGATG CCAGCATGAT GCAGATATCT
    CAGGGCACGA TTGGCACGCC TTGGCCCCAG AGCTACCATT CCAGCTCCTC CACCAGTGAC
    CTCTCCGGCT ATGACCACGC ATATCTGAGG CAGAGCCCTG ACCGGTGCAG CTCCCAGGGG
    AGCATGGAGA GCCTGGAGGC CGGCGGGGGA TACCCACCCT GCCACCTTCT CTCCCCGGCC
    AAGTCCACCA GCAGCATTGA CCAGCTCAGC CACCTCCACA GCAAGAGAGA TTCGGCTTAC
    AGCTCCTTCT CCACCAGTTC CAGCATCCTC GAGTATCCAC CCCCTGGCGT CTCTGGCCGG
    GAGCGTTCGG GCTCCATGGA CACCACTTCT GCTCGAGGTG GCCTCCTAGA AGGGATGAAG
    CAGGCAGATA TTCGCTATAT TAAGACAGTC TATGACCCCC GGAGGGGCGT CTCAGCAGAG
    TATGAGGTGA ACTCTTCAGG CCTGCTTCTC CAAGGTAGGG AGACCCGAGC CTCAACTGAT
    GGTCAGGGCT ACAATAAATT CCATAATGTT CCTCGGGGCA AAGGCGCACC ACCCTCTTCC
    TGGAGCCAGC AGTGCCCTGG TTCCTTAGAG ACCACTGTGG ACAACTTGCC TCCTAAAGTG
    GGTGCGCCCC TGCCCCCAGC TCGGAGTGAC AGTTATGCAG CCTTCCGGCA GCGAGAGAGG
    CCCAGCTCCT GGTCTAGCCT AGATCAGAAA CGGTTCTGCC GACCTCCAGC AAACTCTGCA
    GGCACCCTGA AATCTCCCTT CATAGAGGAA CAGCTGCATA CTGTGCTAGA GAAGAGCCCA
    GAAAACAGCC CTCCAGTGAA GCCCAAGCAC AATTATACCC AGAAGGCCCA GCCCGGCCAA
    CCTCTGCTGC CGACTGGCAT CTACCCGGTA CCTTCCCTGG AGCCACACTT CGCCCAGGTG
    CCCCGGCCTT CTGTGAGTGG AAATGGTACC CTCTACCCTG CCCTGGCCAA GGAGGGTGGG
    TACACACCTC CTCAGGGAGC GTGCGACAGA ATGGCTGCCC TTGATGAGAA TGGGAACCAA
    AACGGATCTA GCAGGCCTGG CTTCGCCTTC TGCCAGGCCT TAGAACGGGA CTCAGTGTCC
    CTAGTAGAGA GGAAACCTGA AACTTCAGCC AAATGTGTCC CCTATAAAGT CCATTTTCCC
    TCAGTGCCTG AGAACGAGGA GGAGACCTCC CTGAAGAGAC TTCTCACACC TCTAGAAGGC
    CACAGCCCAC ATCCCAGTGA GAGAAAGAGC ACCCAGGGCA ACAGATATTC TAATTACCAC
    AGCCTCCAAT CCCCTCAGGC TCAGGTGTGC GAAGTGGGTG AAGACAAGAG ATCCTTCCTG
    CCTTCAGAGC CTTGGGAGGG GGATTCCCAT GAAGACCACA ATGCCAACCT CCGGGGGAGG
    CTCCACGGAG GCAGCCTGGG CCAGAGTATG CCAGGCAGCT TTGGCAAGAC TGCAGCTGCC
    TTCTCCTCCC TCCAGAACAT TCCTGAGAGT CTAAGAAGGC AAAGCAGCTT GGATCTCGGA
    GCAGCAGCCC AGGAAGTCTA CCTGGAAGGC TCATCCACCT GCGCAGTCAA TACCAAGGGA
    GAAGAGTCTG GAAGGACTGC TGTTGCTGAT CACAAGAGCC AGCTGGACAG GTTGGTGTCC
    TATCCAAGGC CCGAGGGGAG AACCTTGGCC TCTTTCCAGA GTTCAGACCC AACGTATGAA
    GAGCCGCCCT CCCCATCCCC ACAGGAGACC TCCAGTCTGG GCCGGAGGAG GCTGAGTTCC
    AGCAGCACCT CGGCTCTGCA GGGCTTTCAG TACGGGAAGC CCCACTGCTC CGTGCTGGAG
    AAGGTTTCCA AGATCGAGCA GCGAGAGCAG GCTGGCCAGA GACCCCCGAG TGCCGGCGGC
    TCTACTTATG GCTATAATTA CAGGCCCCAC AGGACACTCC CAGCCTCCAA TACTTTGGGG
    AATGACTCGG AGGAGACTAA AGGCCGTATA CATTTTTCCG AACTCACTGA ACCCCTAGGC
    AATGGAGAAC AGCACTTCAA AAACGGGGAG CCAAAGTCAG AAGAGGTTTC CTGGCAGCCG
    TGTGGTCAAC AGCCGAGGCG GGCTGTGGAG GGTGGCCGGG GTGCCCCACT CCGGGGTGGA
    GAGCCCCCGA GGCGGGACGC CCGTCTGCTC CGCAGCCAGA GCAGCTTCCA GCTCTTCAGC
    GAGGCCGAGA AGGAGGCCAT GTGGTCAGAC GACAGGCCCC GCACGCCAGA GTCGACTGTG
    CAGGAGGCCC CCTTCAGCCG CGCCTACCGG AACAGCATCA AGGATGCACA GTCCCGCGTC
    CTGGGAGCCA CGTCCTTCCG ACGCCGGGAC CTCGAGCCTG GGACGTCTGT GGCCTCCAGG
    CCCTGGCGCC TGCGACCCGC CTCGGCCCAC GTAGGGCTGC GCAGCCCGGA GGCACCGATT
    TCCGCCTCCC CGCACACCCC GCGCGAGCGG CACAGCGTGA CCCCGGCCGA GGGGGACCCT
    GCCCGCCTCG CGCCCCCTGC TGCCCGGAGG GGCCCGCGCC GGCATCTGAC CACCGAGCAG
    AAGAAGCGCT CGTACTCAGA GCCCGAGAAG ATGCACGAGG TGGGGGTCTC GGAGGAACCC
    GAGCCCGAGC CCCCTGGGCC GCCGAGGAAG GGGCCGCATT TCATGGAGGG CTCAGTGACT
    GACCGGCGCC GCATCTTCGA GCGAGACGGC AGGGCCTGCT CTACCCTCAG CCTGTCGGGG
    CCTGAGCTGA AGCAGTTCCA GCAGAGCGCA CTGGCCGACT ACATTCAGCG CAAGACCGGC
    AAGCGGCCTG CGGGCGCCGG CTGTGGCCTG CAGGAGCCGG GGCGGCTGCT GGAGCGCACC
    CAGAGCACCT ACCTCCAGCC CGAGGGCCCG GGCCTCGCCG CGGCTTCCAG TCTCTGCTCG
    CTTCGGGAGC CCAGCCTGCC GCCCCGCAGG GAGACCGCCC TTCTGCCAGC CACTGTGGTG
    GGGAGTACTG GGGAGACCCA GCGGGGCCCC CGAGATCGCA GCAGCTCGTT TGCCAGCGGC
    CGCCACCTTG GGGAAAGGCA CCGCGGGGAC TCAGCGCCGA GAGAACTGCT CAGTGGAGCT
    AACAATGGAT CAAAGGGCCC CCAGAGACTG AACGGTACCC CAGGGGAACC CTCCCCGTGG
    GGGGCCACAG CTGGGAGGGC CGCGAAGTCC ATGTCGGCTG AGGACCTGCT GGAGCGCTCA
    GACGTCCAGG CTGTCCCTGT CCACATGAGG TCCCGGTCAT CTCCCACCGC CGACAAGCGC
    CAGGATGTGC TTTTGGGGGA AAACAGTAGC TTTGGTCTCA TGAAGGATCC GTGTTATTTG
    ACTGGCCCAG GTTCCAGGCC ATTCAGCTGT TCCAGCCGTG AAGAGATGCC AGCGCTCAGG
    CACCACCCCA GCCCTCACTG GGGGGATTCG GGCTGCAAAG CAGGTAGTGG TGGTGCCTCT
    GTTCCCAGCG GGGGTCCGGG GCCGCTGGAC CCTCCGAGAC AGGCCAGCAG AACGCCTTGC
    CCTCTGCCAC TGTCCTCGGG AGCTCATGGG CATCTTCCAG ACGCCAGGGC TGCACCCCTG
    AGCTCGGTCT TGTCTGCCCC TGGCCCTTCG AGCTATTGCT CACAGGCTGC AGCCCAGCCC
    TCCACACCAG CCGGCACCCC CAGAAGCGGC AGTTGCCACA GCCCGGCCCA GCCTCCCAGC
    CCTGAGCGCA AGACAGAAGA GCTCGCAGGG ATGCGGGCCT CCCCGCCCTG GATGAAGCGG
    ACCTACATTG TCAGAGAGGA GAGCCTCCCC GAGGACCCCC CGAAGCACTA CCCCAAGCCA
    CCGGATGCCC CCAGCTCAAG CAGCACTTCT GACCCGGATA CGCCTCTCGG GGCCCCAGGC
    ATTCTGGGCA GGATTTCCCT CCGGATCTCC GAGTCGGCCT TGCTGGCTTC CCCGCCACCT
    CGCGAGGACT GCGAGGACGA CGAGGTGTTT GTGAGGGACC CACGCCCCAC CGCCACTTCC
    AGCCCCCGCT GCGATGAGCT GCTGCTGCCC CCTCCGCCTC CGCCCCCACC ACCAACAACC
    AGCCAGGCAT CCCTGGCCCA GGGCCTGGAC CACTTCCCTC CGCCTCCTCC CGAAGCTATG
    TGCCCGGCCC AGTGGGAGGA GGGCTACCTG GAGCCCCGTG CCAGCTCCAG CAAACTTGCC
    AAGGTGACAG TTGCCAAGGA AAGGTCCATG GCCGGTGCAG CCCATCCGGT TGATAATCAG
    CTGCTGGGTT CCAGATCGCA AACTTCAGGC AAGAGTTTCG AGGCCAAGGA GACCAACCCA
    CCCTCCACCA CGGGTGCTCT GCCCCAGCCT GCTGGGTCTC TAAGCAAGCA GCCAAGCCCA
    GGACAGCCAC CTCCCATGCA GACCCACAGC CTCATCCACG AGCCAGCCAT TGGGACTGCA
    GGTTTAGAAA AGAATGTCAG TTCTGGTCCT CAAAAGACCT CAGAAGACAT CAGAACAGAG
    ACGCTAGCCA AGGAAATTAT CCACCAAGAC AAATCTCTGG CAGACATTTT GGACCCAGAC
    TCCAGAATGA AGACAACTAT GGACCTGATG GAAGGTTTGT TTCCTCGAGA TGTTAATTTG
    CTGAAGGAAA ACAGCGTAAA GAAGAAGGCC GTGCAGAAAA CCATCAGCTT CCCAGGATGT
    GAGACCAAGA GGAGTGACGA CAAGGAAGCA GTGGGCATGT TGGTCAACTG TCCTGCCTAC
    TACAGCGTCT CTGCTCCCAA GGCTGAGCTT CTCAACAAAA TCAAAGCTAT GCCAGAAGAG
    GTGAACGAGG AAGAGGAGCA GGCAGACATC AACGAAAAGA AGGCGGAGCT GATCGGAAGC
    CTCACCCACA AGCTCGAGAC CCTCCAGGAA GCCAAGGGGA GCCTGCTGAT GGACATCAAG
    CTCAATAACG CCCTGGGGGA GGAGGTGGAG GCCTGGATCA GTGAGCTCTG CAAGCCCAAC
    GAGATCGACA AGTACAAGAT GTTCATCGGG GACTTGGACA AGGTGGTGAA CCTGCTCCTG
    TCCCTCTCGG GGCGCCTGGC CCGCGTGGAG AACGTGCTCA GCGGCCTTGG TGAAGACGCC
    AGTAACGAGG AAAGGAGTTC ACTCAACGAG AAGAGGAAGG TCCTGTCTGC TCAGCACGAG
    GACGCCCGGG AGCTCAAGGA GAACGTGGAT CGGAGGGAGC GCGTGGTGCT GGACATCCTG
    GCCAATTACC TCTCCGAAGA GCAGCTCCAA GATTACCGGC ATTTCGTGAA AATGAAGTCG
    ACACTCCTCA TCGAGCAGCG AGAGCTGGAT GACAAGATCA AGCTGGGCCA GGAGCAGGTC
    AAGTGCCTGC TAGAGAGCCT TCCCTCGGAC TTCGTGCCGA AGGCGGGGGC CCTGGCTCTG
    CCCCCAGGCC TCACCGGCGA TGTGACTCCC GTGGGGGGCT GGACTGGCAG TGGTGTTTTC
    CCAGCGGTGA CCTCCCCACT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235083
    Clone ID Related Accession (Same CDS sequence) XM_010806187.3
    Accession Version XM_010806187.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806187.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCTTTTT ATCCCACTCC TGAGTATGAC ATGCAGGTGG CCAGAGGATT GTTATCTAGA 
    ATGTTTCTCT TTGCCACAAG ACGCACTGAA CCTGCAGGCC GGCCTCATTC TTGGCACTCG
    ACCAAACTTG GGGAGAACCA ACCCGATGCC AGCATGATGC AGATATCTCA GGGCACGATT
    GGCACGCCTT GGCCCCAGAG CTACCATTCC AGCTCCTCCA CCAGTGACCT CTCCGGCTAT
    GACCACGCAT ATCTGAGGCA GAGCCCTGAC CGGTGCAGCT CCCAGGGGAG CATGGAGAGC
    CTGGAGGCCG GCGGGGGATA CCCACCCTGC CACCTTCTCT CCCCGGCCAA GTCCACCAGC
    AGCATTGACC AGCTCAGCCA CCTCCACAGC AAGAGAGATT CGGCTTACAG CTCCTTCTCC
    ACCAGTTCCA GCATCCTCGA GTATCCACCC CCTGGCGTCT CTGGCCGGGA GCGTTCGGGC
    TCCATGGACA CCACTTCTGC TCGAGGTGGC CTCCTAGAAG GGATGAAGCA GGCAGATATT
    CGCTATATTA AGACAGTCTA TGACCCCCGG AGGGGCGTCT CAGCAGAGTA TGAGGTGAAC
    TCTTCAGGCC TGCTTCTCCA AGGTAGGGAG ACCCGAGCCT CAACTGATGG TCAGGGCTAC
    AATAAATTCC ATAATGTTCC TCGGGGCAAA GGCGCACCAC CCTCTTCCTG GAGCCAGCAG
    TGCCCTGGTT CCTTAGAGAC CACTGTGGAC AACTTGCCTC CTAAAGTGGG TGCGCCCCTG
    CCCCCAGCTC GGAGTGACAG TTATGCAGCC TTCCGGCAGC GAGAGAGGCC CAGCTCCTGG
    TCTAGCCTAG ATCAGAAACG GTTCTGCCGA CCTCCAGCAA ACTCTGCAGG CACCCTGAAA
    TCTCCCTTCA TAGAGGAACA GCTGCATACT GTGCTAGAGA AGAGCCCAGA AAACAGCCCT
    CCAGTGAAGC CCAAGCACAA TTATACCCAG AAGGCCCAGC CCGGCCAACC TCTGCTGCCG
    ACTGGCATCT ACCCGGTACC TTCCCTGGAG CCACACTTCG CCCAGGTGCC CCGGCCTTCT
    GTGAGTGGAA ATGGTACCCT CTACCCTGCC CTGGCCAAGG AGGGTGGGTA CACACCTCCT
    CAGGGAGCGT GCGACAGAAT GGCTGCCCTT GATGAGAATG GGAACCAAAA CGGATCTAGC
    AGGCCTGGCT TCGCCTTCTG CCAGGCCTTA GAACGGGACT CAGTGTCCCT AGTAGAGAGG
    AAACCTGAAA CTTCAGCCAA ATGTGTCCCC TATAAAGTCC ATTTTCCCTC AGTGCCTGAG
    AACGAGGAGG AGACCTCCCT GAAGAGACTT CTCACACCTC TAGAAGGCCA CAGCCCACAT
    CCCAGTGAGA GAAAGAGCAC CCAGGGCAAC AGATATTCTA ATTACCACAG CCTCCAATCC
    CCTCAGGCTC AGGTGTGCGA AGTGGGTGAA GACAAGAGAT CCTTCCTGCC TTCAGAGCCT
    TGGGAGGGGG ATTCCCATGA AGACCACAAT GCCAACCTCC GGGGGAGGCT CCACGGAGGC
    AGCCTGGGCC AGAGTATGCC AGGCAGCTTT GGCAAGACTG CAGCTGCCTT CTCCTCCCTC
    CAGAACATTC CTGAGAGTCT AAGAAGGCAA AGCAGCTTGG ATCTCGGAGC AGCAGCCCAG
    GAAGTCTACC TGGAAGGCTC ATCCACCTGC GCAGTCAATA CCAAGGGAGA AGAGTCTGGA
    AGGACTGCTG TTGCTGATCA CAAGAGCCAG CTGGACAGGT TGGTGTCCTA TCCAAGGCCC
    GAGGGGAGAA CCTTGGCCTC TTTCCAGAGT TCAGACCCAA CGTATGAAGA GCCGCCCTCC
    CCATCCCCAC AGGAGACCTC CAGTCTGGGC CGGAGGAGGC TGAGTTCCAG CAGCACCTCG
    GCTCTGCAGG GCTTTCAGTA CGGGAAGCCC CACTGCTCCG TGCTGGAGAA GGTTTCCAAG
    ATCGAGCAGC GAGAGCAGGC TGGCCAGAGA CCCCCGAGTG CCGGCGGCTC TACTTATGGC
    TATAATTACA GGCCCCACAG GACACTCCCA GCCTCCAATA CTTTGGGGAA TGACTCGGAG
    GAGACTAAAG GCCGTATACA TTTTTCCGAA CTCACTGAAC CCCTAGGCAA TGGAGAACAG
    CACTTCAAAA ACGGGGAGCC AAAGTCAGAA GAGGTTTCCT GGCAGCCGTG TGGTCAACAG
    CCGAGGCGGG CTGTGGAGGG TGGCCGGGGT GCCCCACTCC GGGGTGGAGA GCCCCCGAGG
    CGGGACGCCC GTCTGCTCCG CAGCCAGAGC AGCTTCCAGC TCTTCAGCGA GGCCGAGAAG
    GAGGCCATGT GGTCAGACGA CAGGCCCCGC ACGCCAGAGT CGACTGTGCA GGAGGCCCCC
    TTCAGCCGCG CCTACCGGAA CAGCATCAAG GATGCACAGT CCCGCGTCCT GGGAGCCACG
    TCCTTCCGAC GCCGGGACCT CGAGCCTGGG ACGTCTGTGG CCTCCAGGCC CTGGCGCCTG
    CGACCCGCCT CGGCCCACGT AGGGCTGCGC AGCCCGGAGG CACCGATTTC CGCCTCCCCG
    CACACCCCGC GCGAGCGGCA CAGCGTGACC CCGGCCGAGG GGGACCCTGC CCGCCTCGCG
    CCCCCTGCTG CCCGGAGGGG CCCGCGCCGG CATCTGACCA CCGAGCAGAA GAAGCGCTCG
    TACTCAGAGC CCGAGAAGAT GCACGAGGTG GGGGTCTCGG AGGAACCCGA GCCCGAGCCC
    CCTGGGCCGC CGAGGAAGGG GCCGCATTTC ATGGAGGGCT CAGTGACTGA CCGGCGCCGC
    ATCTTCGAGC GAGACGGCAG GGCCTGCTCT ACCCTCAGCC TGTCGGGGCC TGAGCTGAAG
    CAGTTCCAGC AGAGCGCACT GGCCGACTAC ATTCAGCGCA AGACCGGCAA GCGGCCTGCG
    GGCGCCGGCT GTGGCCTGCA GGAGCCGGGG CGGCTGCTGG AGCGCACCCA GAGCACCTAC
    CTCCAGCCCG AGGGCCCGGG CCTCGCCGCG GCTTCCAGTC TCTGCTCGCT TCGGGAGCCC
    AGCCTGCCGC CCCGCAGGGA GACCGCCCTT CTGCCAGCCA CTGTGGTGGG GAGTACTGGG
    GAGACCCAGC GGGGCCCCCG AGATCGCAGC AGCTCGTTTG CCAGCGGCCG CCACCTTGGG
    GAAAGGCACC GCGGGGACTC AGCGCCGAGA GAACTGCTCA GTGGAGCTAA CAATGGATCA
    AAGGGCCCCC AGAGACTGAA CGGTACCCCA GGGGAACCCT CCCCGTGGGG GGCCACAGCT
    GGGAGGGCCG CGAAGTCCAT GTCGGCTGAG GACCTGCTGG AGCGCTCAGA CGTCCAGGCT
    GTCCCTGTCC ACATGAGGTC CCGGTCATCT CCCACCGCCG ACAAGCGCCA GGATGTGCTT
    TTGGGGGAAA ACAGTAGCTT TGGTCTCATG AAGGATCCGT GTTATTTGAC TGGCCCAGGT
    TCCAGGCCAT TCAGCTGTTC CAGCCGTGAA GAGATGCCAG CGCTCAGGCA CCACCCCAGC
    CCTCACTGGG GGGATTCGGG CTGCAAAGCA GGTAGTGGTG GTGCCTCTGT TCCCAGCGGG
    GGTCCGGGGC CGCTGGACCC TCCGAGACAG GCCAGCAGAA CGCCTTGCCC TCTGCCACTG
    TCCTCGGGAG CTCATGGGCA TCTTCCAGAC GCCAGGGCTG CACCCCTGAG CTCGGTCTTG
    TCTGCCCCTG GCCCTTCGAG CTATTGCTCA CAGGCTGCAG CCCAGCCCTC CACACCAGCC
    GGCACCCCCA GAAGCGGCAG TTGCCACAGC CCGGCCCAGC CTCCCAGCCC TGAGCGCAAG
    ACAGAAGAGC TCGCAGGGAT GCGGGCCTCC CCGCCCTGGA TGAAGCGGAC CTACATTGTC
    AGAGAGGAGA GCCTCCCCGA GGACCCCCCG AAGCACTACC CCAAGCCACC GGATGCCCCC
    AGCTCAAGCA GCACTTCTGA CCCGGATACG CCTCTCGGGG CCCCAGGCAT TCTGGGCAGG
    ATTTCCCTCC GGATCTCCGA GTCGGCCTTG CTGGCTTCCC CGCCACCTCG CGAGGACTGC
    GAGGACGACG AGGTGTTTGT GAGGGACCCA CGCCCCACCG CCACTTCCAG CCCCCGCTGC
    GATGAGCTGC TGCTGCCCCC TCCGCCTCCG CCCCCACCAC CAACAACCAG CCAGGCATCC
    CTGGCCCAGG GCCTGGACCA CTTCCCTCCG CCTCCTCCCG AAGCTATGTG CCCGGCCCAG
    TGGGAGGAGG GCTACCTGGA GCCCCGTGCC AGCTCCAGCA AACTTGCCAA GGTGACAGTT
    GCCAAGGAAA GGTCCATGGC CGGTGCAGCC CATCCGGTTG ATAATCAGCT GCTGGGTTCC
    AGATCGCAAA CTTCAGGCAA GAGTTTCGAG GCCAAGGAGA CCAACCCACC CTCCACCACG
    GGTGCTCTGC CCCAGCCTGC TGGGTCTCTA AGCAAGCAGC CAAGCCCAGG ACAGCCACCT
    CCCATGCAGA CCCACAGCCT CATCCACGAG CCAGCCATTG GGACTGCAGG TTTAGAAAAG
    AATGTCAGTT CTGGTCCTCA AAAGACCTCA GAAGACATCA GAACAGAGAC GCTAGCCAAG
    GAAATTATCC ACCAAGACAA ATCTCTGGCA GACATTTTGG ACCCAGACTC CAGAATGAAG
    ACAACTATGG ACCTGATGGA AGGTTTGTTT CCTCGAGATG TTAATTTGCT GAAGGAAAAC
    AGCGTAAAGA AGAAGGCCGT GCAGAAAACC ATCAGCTTCC CAGGATGTGA GACCAAGAGG
    AGTGACGACA AGGAAGCAGT GGGCATGTTG GTCAACTGTC CTGCCTACTA CAGCGTCTCT
    GCTCCCAAGG CTGAGCTTCT CAACAAAATC AAAGCTATGC CAGAAGAGGT GAACGAGGAA
    GAGGAGCAGG CAGACATCAA CGAAAAGAAG GCGGAGCTGA TCGGAAGCCT CACCCACAAG
    CTCGAGACCC TCCAGGAAGC CAAGGGGAGC CTGCTGATGG ACATCAAGCT CAATAACGCC
    CTGGGGGAGG AGGTGGAGGC CTGGATCAGT GAGCTCTGCA AGCCCAACGA GATCGACAAG
    TACAAGATGT TCATCGGGGA CTTGGACAAG GTGGTGAACC TGCTCCTGTC CCTCTCGGGG
    CGCCTGGCCC GCGTGGAGAA CGTGCTCAGC GGCCTTGGTG AAGACGCCAG TAACGAGGAA
    AGGAGTTCAC TCAACGAGAA GAGGAAGGTC CTGTCTGCTC AGCACGAGGA CGCCCGGGAG
    CTCAAGGAGA ACGTGGATCG GAGGGAGCGC GTGGTGCTGG ACATCCTGGC CAATTACCTC
    TCCGAAGAGC AGCTCCAAGA TTACCGGCAT TTCGTGAAAA TGAAGTCGAC ACTCCTCATC
    GAGCAGCGAG AGCTGGATGA CAAGATCAAG CTGGGCCAGG AGCAGGTCAA GTGCCTGCTA
    GAGAGCCTTC CCTCGGACTT CGTGCCGAAG GCGGGGGCCC TGGCTCTGCC CCCAGGCCTC
    ACCGGCGATG TGACTCCCGT GGGGGGCTGG ACTGGCAGTG GTGTTTTCCC AGCGGTGACC
    TCCCCACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804489.1
    CDS47..5638
    Translation

    Target ORF information:

    RefSeq Version XM_010806187.3
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806187.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCTTTTT ATCCCACTCC TGAGTATGAC ATGCAGGTGG CCAGAGGATT GTTATCTAGA 
    ATGTTTCTCT TTGCCACAAG ACGCACTGAA CCTGCAGGCC GGCCTCATTC TTGGCACTCG
    ACCAAACTTG GGGAGAACCA ACCCGATGCC AGCATGATGC AGATATCTCA GGGCACGATT
    GGCACGCCTT GGCCCCAGAG CTACCATTCC AGCTCCTCCA CCAGTGACCT CTCCGGCTAT
    GACCACGCAT ATCTGAGGCA GAGCCCTGAC CGGTGCAGCT CCCAGGGGAG CATGGAGAGC
    CTGGAGGCCG GCGGGGGATA CCCACCCTGC CACCTTCTCT CCCCGGCCAA GTCCACCAGC
    AGCATTGACC AGCTCAGCCA CCTCCACAGC AAGAGAGATT CGGCTTACAG CTCCTTCTCC
    ACCAGTTCCA GCATCCTCGA GTATCCACCC CCTGGCGTCT CTGGCCGGGA GCGTTCGGGC
    TCCATGGACA CCACTTCTGC TCGAGGTGGC CTCCTAGAAG GGATGAAGCA GGCAGATATT
    CGCTATATTA AGACAGTCTA TGACCCCCGG AGGGGCGTCT CAGCAGAGTA TGAGGTGAAC
    TCTTCAGGCC TGCTTCTCCA AGGTAGGGAG ACCCGAGCCT CAACTGATGG TCAGGGCTAC
    AATAAATTCC ATAATGTTCC TCGGGGCAAA GGCGCACCAC CCTCTTCCTG GAGCCAGCAG
    TGCCCTGGTT CCTTAGAGAC CACTGTGGAC AACTTGCCTC CTAAAGTGGG TGCGCCCCTG
    CCCCCAGCTC GGAGTGACAG TTATGCAGCC TTCCGGCAGC GAGAGAGGCC CAGCTCCTGG
    TCTAGCCTAG ATCAGAAACG GTTCTGCCGA CCTCCAGCAA ACTCTGCAGG CACCCTGAAA
    TCTCCCTTCA TAGAGGAACA GCTGCATACT GTGCTAGAGA AGAGCCCAGA AAACAGCCCT
    CCAGTGAAGC CCAAGCACAA TTATACCCAG AAGGCCCAGC CCGGCCAACC TCTGCTGCCG
    ACTGGCATCT ACCCGGTACC TTCCCTGGAG CCACACTTCG CCCAGGTGCC CCGGCCTTCT
    GTGAGTGGAA ATGGTACCCT CTACCCTGCC CTGGCCAAGG AGGGTGGGTA CACACCTCCT
    CAGGGAGCGT GCGACAGAAT GGCTGCCCTT GATGAGAATG GGAACCAAAA CGGATCTAGC
    AGGCCTGGCT TCGCCTTCTG CCAGGCCTTA GAACGGGACT CAGTGTCCCT AGTAGAGAGG
    AAACCTGAAA CTTCAGCCAA ATGTGTCCCC TATAAAGTCC ATTTTCCCTC AGTGCCTGAG
    AACGAGGAGG AGACCTCCCT GAAGAGACTT CTCACACCTC TAGAAGGCCA CAGCCCACAT
    CCCAGTGAGA GAAAGAGCAC CCAGGGCAAC AGATATTCTA ATTACCACAG CCTCCAATCC
    CCTCAGGCTC AGGTGTGCGA AGTGGGTGAA GACAAGAGAT CCTTCCTGCC TTCAGAGCCT
    TGGGAGGGGG ATTCCCATGA AGACCACAAT GCCAACCTCC GGGGGAGGCT CCACGGAGGC
    AGCCTGGGCC AGAGTATGCC AGGCAGCTTT GGCAAGACTG CAGCTGCCTT CTCCTCCCTC
    CAGAACATTC CTGAGAGTCT AAGAAGGCAA AGCAGCTTGG ATCTCGGAGC AGCAGCCCAG
    GAAGTCTACC TGGAAGGCTC ATCCACCTGC GCAGTCAATA CCAAGGGAGA AGAGTCTGGA
    AGGACTGCTG TTGCTGATCA CAAGAGCCAG CTGGACAGGT TGGTGTCCTA TCCAAGGCCC
    GAGGGGAGAA CCTTGGCCTC TTTCCAGAGT TCAGACCCAA CGTATGAAGA GCCGCCCTCC
    CCATCCCCAC AGGAGACCTC CAGTCTGGGC CGGAGGAGGC TGAGTTCCAG CAGCACCTCG
    GCTCTGCAGG GCTTTCAGTA CGGGAAGCCC CACTGCTCCG TGCTGGAGAA GGTTTCCAAG
    ATCGAGCAGC GAGAGCAGGC TGGCCAGAGA CCCCCGAGTG CCGGCGGCTC TACTTATGGC
    TATAATTACA GGCCCCACAG GACACTCCCA GCCTCCAATA CTTTGGGGAA TGACTCGGAG
    GAGACTAAAG GCCGTATACA TTTTTCCGAA CTCACTGAAC CCCTAGGCAA TGGAGAACAG
    CACTTCAAAA ACGGGGAGCC AAAGTCAGAA GAGGTTTCCT GGCAGCCGTG TGGTCAACAG
    CCGAGGCGGG CTGTGGAGGG TGGCCGGGGT GCCCCACTCC GGGGTGGAGA GCCCCCGAGG
    CGGGACGCCC GTCTGCTCCG CAGCCAGAGC AGCTTCCAGC TCTTCAGCGA GGCCGAGAAG
    GAGGCCATGT GGTCAGACGA CAGGCCCCGC ACGCCAGAGT CGACTGTGCA GGAGGCCCCC
    TTCAGCCGCG CCTACCGGAA CAGCATCAAG GATGCACAGT CCCGCGTCCT GGGAGCCACG
    TCCTTCCGAC GCCGGGACCT CGAGCCTGGG ACGTCTGTGG CCTCCAGGCC CTGGCGCCTG
    CGACCCGCCT CGGCCCACGT AGGGCTGCGC AGCCCGGAGG CACCGATTTC CGCCTCCCCG
    CACACCCCGC GCGAGCGGCA CAGCGTGACC CCGGCCGAGG GGGACCCTGC CCGCCTCGCG
    CCCCCTGCTG CCCGGAGGGG CCCGCGCCGG CATCTGACCA CCGAGCAGAA GAAGCGCTCG
    TACTCAGAGC CCGAGAAGAT GCACGAGGTG GGGGTCTCGG AGGAACCCGA GCCCGAGCCC
    CCTGGGCCGC CGAGGAAGGG GCCGCATTTC ATGGAGGGCT CAGTGACTGA CCGGCGCCGC
    ATCTTCGAGC GAGACGGCAG GGCCTGCTCT ACCCTCAGCC TGTCGGGGCC TGAGCTGAAG
    CAGTTCCAGC AGAGCGCACT GGCCGACTAC ATTCAGCGCA AGACCGGCAA GCGGCCTGCG
    GGCGCCGGCT GTGGCCTGCA GGAGCCGGGG CGGCTGCTGG AGCGCACCCA GAGCACCTAC
    CTCCAGCCCG AGGGCCCGGG CCTCGCCGCG GCTTCCAGTC TCTGCTCGCT TCGGGAGCCC
    AGCCTGCCGC CCCGCAGGGA GACCGCCCTT CTGCCAGCCA CTGTGGTGGG GAGTACTGGG
    GAGACCCAGC GGGGCCCCCG AGATCGCAGC AGCTCGTTTG CCAGCGGCCG CCACCTTGGG
    GAAAGGCACC GCGGGGACTC AGCGCCGAGA GAACTGCTCA GTGGAGCTAA CAATGGATCA
    AAGGGCCCCC AGAGACTGAA CGGTACCCCA GGGGAACCCT CCCCGTGGGG GGCCACAGCT
    GGGAGGGCCG CGAAGTCCAT GTCGGCTGAG GACCTGCTGG AGCGCTCAGA CGTCCAGGCT
    GTCCCTGTCC ACATGAGGTC CCGGTCATCT CCCACCGCCG ACAAGCGCCA GGATGTGCTT
    TTGGGGGAAA ACAGTAGCTT TGGTCTCATG AAGGATCCGT GTTATTTGAC TGGCCCAGGT
    TCCAGGCCAT TCAGCTGTTC CAGCCGTGAA GAGATGCCAG CGCTCAGGCA CCACCCCAGC
    CCTCACTGGG GGGATTCGGG CTGCAAAGCA GGTAGTGGTG GTGCCTCTGT TCCCAGCGGG
    GGTCCGGGGC CGCTGGACCC TCCGAGACAG GCCAGCAGAA CGCCTTGCCC TCTGCCACTG
    TCCTCGGGAG CTCATGGGCA TCTTCCAGAC GCCAGGGCTG CACCCCTGAG CTCGGTCTTG
    TCTGCCCCTG GCCCTTCGAG CTATTGCTCA CAGGCTGCAG CCCAGCCCTC CACACCAGCC
    GGCACCCCCA GAAGCGGCAG TTGCCACAGC CCGGCCCAGC CTCCCAGCCC TGAGCGCAAG
    ACAGAAGAGC TCGCAGGGAT GCGGGCCTCC CCGCCCTGGA TGAAGCGGAC CTACATTGTC
    AGAGAGGAGA GCCTCCCCGA GGACCCCCCG AAGCACTACC CCAAGCCACC GGATGCCCCC
    AGCTCAAGCA GCACTTCTGA CCCGGATACG CCTCTCGGGG CCCCAGGCAT TCTGGGCAGG
    ATTTCCCTCC GGATCTCCGA GTCGGCCTTG CTGGCTTCCC CGCCACCTCG CGAGGACTGC
    GAGGACGACG AGGTGTTTGT GAGGGACCCA CGCCCCACCG CCACTTCCAG CCCCCGCTGC
    GATGAGCTGC TGCTGCCCCC TCCGCCTCCG CCCCCACCAC CAACAACCAG CCAGGCATCC
    CTGGCCCAGG GCCTGGACCA CTTCCCTCCG CCTCCTCCCG AAGCTATGTG CCCGGCCCAG
    TGGGAGGAGG GCTACCTGGA GCCCCGTGCC AGCTCCAGCA AACTTGCCAA GGTGACAGTT
    GCCAAGGAAA GGTCCATGGC CGGTGCAGCC CATCCGGTTG ATAATCAGCT GCTGGGTTCC
    AGATCGCAAA CTTCAGGCAA GAGTTTCGAG GCCAAGGAGA CCAACCCACC CTCCACCACG
    GGTGCTCTGC CCCAGCCTGC TGGGTCTCTA AGCAAGCAGC CAAGCCCAGG ACAGCCACCT
    CCCATGCAGA CCCACAGCCT CATCCACGAG CCAGCCATTG GGACTGCAGG TTTAGAAAAG
    AATGTCAGTT CTGGTCCTCA AAAGACCTCA GAAGACATCA GAACAGAGAC GCTAGCCAAG
    GAAATTATCC ACCAAGACAA ATCTCTGGCA GACATTTTGG ACCCAGACTC CAGAATGAAG
    ACAACTATGG ACCTGATGGA AGGTTTGTTT CCTCGAGATG TTAATTTGCT GAAGGAAAAC
    AGCGTAAAGA AGAAGGCCGT GCAGAAAACC ATCAGCTTCC CAGGATGTGA GACCAAGAGG
    AGTGACGACA AGGAAGCAGT GGGCATGTTG GTCAACTGTC CTGCCTACTA CAGCGTCTCT
    GCTCCCAAGG CTGAGCTTCT CAACAAAATC AAAGCTATGC CAGAAGAGGT GAACGAGGAA
    GAGGAGCAGG CAGACATCAA CGAAAAGAAG GCGGAGCTGA TCGGAAGCCT CACCCACAAG
    CTCGAGACCC TCCAGGAAGC CAAGGGGAGC CTGCTGATGG ACATCAAGCT CAATAACGCC
    CTGGGGGAGG AGGTGGAGGC CTGGATCAGT GAGCTCTGCA AGCCCAACGA GATCGACAAG
    TACAAGATGT TCATCGGGGA CTTGGACAAG GTGGTGAACC TGCTCCTGTC CCTCTCGGGG
    CGCCTGGCCC GCGTGGAGAA CGTGCTCAGC GGCCTTGGTG AAGACGCCAG TAACGAGGAA
    AGGAGTTCAC TCAACGAGAA GAGGAAGGTC CTGTCTGCTC AGCACGAGGA CGCCCGGGAG
    CTCAAGGAGA ACGTGGATCG GAGGGAGCGC GTGGTGCTGG ACATCCTGGC CAATTACCTC
    TCCGAAGAGC AGCTCCAAGA TTACCGGCAT TTCGTGAAAA TGAAGTCGAC ACTCCTCATC
    GAGCAGCGAG AGCTGGATGA CAAGATCAAG CTGGGCCAGG AGCAGGTCAA GTGCCTGCTA
    GAGAGCCTTC CCTCGGACTT CGTGCCGAAG GCGGGGGCCC TGGCTCTGCC CCCAGGCCTC
    ACCGGCGATG TGACTCCCGT GGGGGGCTGG ACTGGCAGTG GTGTTTTCCC AGCGGTGACC
    TCCCCACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235084
    Clone ID Related Accession (Same CDS sequence) XM_010806188.3 , XM_010806189.3 , XM_024992949.1 , XM_010806190.3
    Accession Version XM_010806188.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCGAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804490.1
    CDS388..5826
    Translation

    Target ORF information:

    RefSeq Version XM_010806188.3
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806188.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCGAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235084
    Clone ID Related Accession (Same CDS sequence) XM_010806188.3 , XM_010806189.3 , XM_024992949.1 , XM_010806190.3
    Accession Version XM_010806189.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCGAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804491.1
    CDS394..5832
    Translation

    Target ORF information:

    RefSeq Version XM_010806189.3
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806189.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCGAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235084
    Clone ID Related Accession (Same CDS sequence) XM_010806188.3 , XM_010806189.3 , XM_024992949.1 , XM_010806190.3
    Accession Version XM_024992949.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCGAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848717.1
    CDS584..6022
    Translation

    Target ORF information:

    RefSeq Version XM_024992949.1
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992949.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCGAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235084
    Clone ID Related Accession (Same CDS sequence) XM_010806188.3 , XM_010806189.3 , XM_024992949.1 , XM_010806190.3
    Accession Version XM_010806190.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCGAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804492.1
    CDS396..5834
    Translation

    Target ORF information:

    RefSeq Version XM_010806190.3
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806190.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCGAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235081
    Clone ID Related Accession (Same CDS sequence) XM_024992948.1
    Accession Version XM_024992948.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6027bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGAGGTCG TGAGTGGCTT TTGGGTGGAA ATCGAGTTCT TTCAGCCCTT CACTCTGTTT 
    CTGAATGCCC TCTGTGGCTG GCTGGGTGCT GGGTGCTGGA AGGTGTGGAG ACAGGAGGTC
    ATCCCTGCCT TCTGGATGCA AACGGTGGGA GTGGGCGTGG CCACAGGCAG TGGGCGGGCT
    CTGCGGGAAA CAGGCTGCTC ACCTGTGGAT CCTCCTCTCT CTAAGCAGAT CAAAGAAGGG
    GGCAAAGCGG ACCTGGTGAG CTCCAGACTT CGGGCTGGGG ACGAGGTGGT GAACATCAAC
    GAGGTGGCAC TGAGCAGCTC CCGGAGGGAG GCCGTTTCCT TGGTGAAAGG ATCCTACAAG
    ACTCTGCGGC TGGTGGTGCG CAGAGATGCA CATGCAGACC CGGGCCCTGC AGACACTGGC
    ACCTCTGACT CATTCGGCCC AGAGCACCTC ACCTCTGACC CCCAGCACAG AAAAGTAGCA
    TGGTCAGGAG GGGTTAAACC TCGGCTGAAG CACAGACGCA CTGAACCTGC AGGCCGGCCT
    CATTCTTGGC ACTCGACCAA ACTTGGGGAG AACCAACCCG ATGCCAGCAT GATGCAGATA
    TCTCAGGGCA CGATTGGCAC GCCTTGGCCC CAGAGCTACC ATTCCAGCTC CTCCACCAGT
    GACCTCTCCG GCTATGACCA CGCATATCTG AGGCAGAGCC CTGACCGGTG CAGCTCCCAG
    GGGAGCATGG AGAGCCTGGA GGCCGGCGGG GGATACCCAC CCTGCCACCT TCTCTCCCCG
    GCCAAGTCCA CCAGCAGCAT TGACCAGCTC AGCCACCTCC ACAGCAAGAG AGATTCGGCT
    TACAGCTCCT TCTCCACCAG TTCCAGCATC CTCGAGTATC CACCCCCTGG CGTCTCTGGC
    CGGGAGCGTT CGGGCTCCAT GGACACCACT TCTGCTCGAG GTGGCCTCCT AGAAGGGATG
    AAGCAGGCAG ATATTCGCTA TATTAAGACA GTCTATGACC CCCGGAGGGG CGTCTCAGCA
    GAGTATGAGG TGAACTCTTC AGGCCTGCTT CTCCAAGGTA GGGAGACCCG AGCCTCAACT
    GATGGTCAGG GCTACAATAA ATTCCATAAT GTTCCTCGGG GCAAAGGCGC ACCACCCTCT
    TCCTGGAGCC AGCAGTGCCC TGGTTCCTTA GAGACCACTG TGGACAACTT GCCTCCTAAA
    GTGGGTGCGC CCCTGCCCCC AGCTCGGAGT GACAGTTATG CAGCCTTCCG GCAGCGAGAG
    AGGCCCAGCT CCTGGTCTAG CCTAGATCAG AAACGGTTCT GCCGACCTCC AGCAAACTCT
    GCAGGCACCC TGAAATCTCC CTTCATAGAG GAACAGCTGC ATACTGTGCT AGAGAAGAGC
    CCAGAAAACA GCCCTCCAGT GAAGCCCAAG CACAATTATA CCCAGAAGGC CCAGCCCGGC
    CAACCTCTGC TGCCGACTGG CATCTACCCG GTACCTTCCC TGGAGCCACA CTTCGCCCAG
    GTGCCCCGGC CTTCTGTGAG TGGAAATGGT ACCCTCTACC CTGCCCTGGC CAAGGAGGGT
    GGGTACACAC CTCCTCAGGG AGCGTGCGAC AGAATGGCTG CCCTTGATGA GAATGGGAAC
    CAAAACGGAT CTAGCAGGCC TGGCTTCGCC TTCTGCCAGG CCTTAGAACG GGACTCAGTG
    TCCCTAGTAG AGAGGAAACC TGAAACTTCA GCCAAATGTG TCCCCTATAA AGTCCATTTT
    CCCTCAGTGC CTGAGAACGA GGAGGAGACC TCCCTGAAGA GACTTCTCAC ACCTCTAGAA
    GGCCACAGCC CACATCCCAG TGAGAGAAAG AGCACCCAGG GCAACAGATA TTCTAATTAC
    CACAGCCTCC AATCCCCTCA GGCTCAGGTG TGCGAAGTGG GTGAAGACAA GAGATCCTTC
    CTGCCTTCAG AGCCTTGGGA GGGGGATTCC CATGAAGACC ACAATGCCAA CCTCCGGGGG
    AGGCTCCACG GAGGCAGCCT GGGCCAGAGT ATGCCAGGCA GCTTTGGCAA GACTGCAGCT
    GCCTTCTCCT CCCTCCAGAA CATTCCTGAG AGTCTAAGAA GGCAAAGCAG CTTGGATCTC
    GGAGCAGCAG CCCAGGAAGT CTACCTGGAA GGCTCATCCA CCTGCGCAGT CAATACCAAG
    GGAGAAGAGT CTGGAAGGAC TGCTGTTGCT GATCACAAGA GCCAGCTGGA CAGGTTGGTG
    TCCTATCCAA GGCCCGAGGG GAGAACCTTG GCCTCTTTCC AGAGTTCAGA CCCAACGTAT
    GAAGAGCCGC CCTCCCCATC CCCACAGGAG ACCTCCAGTC TGGGCCGGAG GAGGCTGAGT
    TCCAGCAGCA CCTCGGCTCT GCAGGGCTTT CAGTACGGGA AGCCCCACTG CTCCGTGCTG
    GAGAAGGTTT CCAAGATCGA GCAGCGAGAG CAGGCTGGCC AGAGACCCCC GAGTGCCGGC
    GGCTCTACTT ATGGCTATAA TTACAGGCCC CACAGGACAC TCCCAGCCTC CAATACTTTG
    GGGAATGACT CGGAGGAGAC TAAAGGCCGT ATACATTTTT CCGAACTCAC TGAACCCCTA
    GGCAATGGAG AACAGCACTT CAAAAACGGG GAGCCAAAGT CAGAAGAGGT TTCCTGGCAG
    CCGTGTGGTC AACAGCCGAG GCGGGCTGTG GAGGGTGGCC GGGGTGCCCC ACTCCGGGGT
    GGAGAGCCCC CGAGGCGGGA CGCCCGTCTG CTCCGCAGCC AGAGCAGCTT CCAGCTCTTC
    AGCGAGGCCG AGAAGGAGGC CATGTGGTCA GACGACAGGC CCCGCACGCC AGAGTCGACT
    GTGCAGGAGG CCCCCTTCAG CCGCGCCTAC CGGAACAGCA TCAAGGATGC ACAGTCCCGC
    GTCCTGGGAG CCACGTCCTT CCGACGCCGG GACCTCGAGC CTGGGACGTC TGTGGCCTCC
    AGGCCCTGGC GCCTGCGACC CGCCTCGGCC CACGTAGGGC TGCGCAGCCC GGAGGCACCG
    ATTTCCGCCT CCCCGCACAC CCCGCGCGAG CGGCACAGCG TGACCCCGGC CGAGGGGGAC
    CCTGCCCGCC TCGCGCCCCC TGCTGCCCGG AGGGGCCCGC GCCGGCATCT GACCACCGAG
    CAGAAGAAGC GCTCGTACTC AGAGCCCGAG AAGATGCACG AGGTGGGGGT CTCGGAGGAA
    CCCGAGCCCG AGCCCCCTGG GCCGCCGAGG AAGGGGCCGC ATTTCATGGA GGGCTCAGTG
    ACTGACCGGC GCCGCATCTT CGAGCGAGAC GGCAGGGCCT GCTCTACCCT CAGCCTGTCG
    GGGCCTGAGC TGAAGCAGTT CCAGCAGAGC GCACTGGCCG ACTACATTCA GCGCAAGACC
    GGCAAGCGGC CTGCGGGCGC CGGCTGTGGC CTGCAGGAGC CGGGGCGGCT GCTGGAGCGC
    ACCCAGAGCA CCTACCTCCA GCCCGAGGGC CCGGGCCTCG CCGCGGCTTC CAGTCTCTGC
    TCGCTTCGGG AGCCCAGCCT GCCGCCCCGC AGGGAGACCG CCCTTCTGCC AGCCACTGTG
    GTGGGGAGTA CTGGGGAGAC CCAGCGGGGC CCCCGAGATC GCAGCAGCTC GTTTGCCAGC
    GGCCGCCACC TTGGGGAAAG GCACCGCGGG GACTCAGCGC CGAGAGAACT GCTCAGTGGA
    GCTAACAATG GATCAAAGGG CCCCCAGAGA CTGAACGGTA CCCCAGGGGA ACCCTCCCCG
    TGGGGGGCCA CAGCTGGGAG GGCCGCGAAG TCCATGTCGG CTGAGGACCT GCTGGAGCGC
    TCAGACGTCC AGGCTGTCCC TGTCCACATG AGGTCCCGGT CATCTCCCAC CGCCGACAAG
    CGCCAGGATG TGCTTTTGGG GGAAAACAGT AGCTTTGGTC TCATGAAGGA TCCGTGTTAT
    TTGACTGGCC CAGGTTCCAG GCCATTCAGC TGTTCCAGCC GTGAAGAGAT GCCAGCGCTC
    AGGCACCACC CCAGCCCTCA CTGGGGGGAT TCGGGCTGCA AAGCAGGTAG TGGTGGTGCC
    TCTGTTCCCA GCGGGGGTCC GGGGCCGCTG GACCCTCCGA GACAGGCCAG CAGAACGCCT
    TGCCCTCTGC CACTGTCCTC GGGAGCTCAT GGGCATCTTC CAGACGCCAG GGCTGCACCC
    CTGAGCTCGG TCTTGTCTGC CCCTGGCCCT TCGAGCTATT GCTCACAGGC TGCAGCCCAG
    CCCTCCACAC CAGCCGGCAC CCCCAGAAGC GGCAGTTGCC ACAGCCCGGC CCAGCCTCCC
    AGCCCTGAGC GCAAGACAGA AGAGCTCGCA GGGATGCGGG CCTCCCCGCC CTGGATGAAG
    CGGACCTACA TTGTCAGAGA GGAGAGCCTC CCCGAGGACC CCCCGAAGCA CTACCCCAAG
    CCACCGGATG CCCCCAGCTC AAGCAGCACT TCTGACCCGG ATACGCCTCT CGGGGCCCCA
    GGCATTCTGG GCAGGATTTC CCTCCGGATC TCCGAGTCGG CCTTGCTGGC TTCCCCGCCA
    CCTCGCGAGG ACTGCGAGGA CGACGAGGTG TTTGTGAGGG ACCCACGCCC CACCGCCACT
    TCCAGCCCCC GCTGCGATGA GCTGCTGCTG CCCCCTCCGC CTCCGCCCCC ACCACCAACA
    ACCAGCCAGG CATCCCTGGC CCAGGGCCTG GACCACTTCC CTCCGCCTCC TCCCGAAGCT
    ATGTGCCCGG CCCAGTGGGA GGAGGGCTAC CTGGAGCCCC GTGCCAGCTC CAGCAAACTT
    GCCAAGGTGA CAGTTGCCAA GGAAAGGTCC ATGGCCGGTG CAGCCCATCC GGTTGATAAT
    CAGCTGCTGG GTTCCAGATC GCAAACTTCA GGCAAGAGTT TCGAGGCCAA GGAGACCAAC
    CCACCCTCCA CCACGGGTGC TCTGCCCCAG CCTGCTGGGT CTCTAAGCAA GCAGCCAAGC
    CCAGGACAGC CACCTCCCAT GCAGACCCAC AGCCTCATCC ACGAGCCAGC CATTGGGACT
    GCAGGTTTAG AAAAGAATGT CAGTTCTGGT CCTCAAAAGA CCTCAGAAGA CATCAGAACA
    GAGACGCTAG CCAAGGAAAT TATCCACCAA GACAAATCTC TGGCAGACAT TTTGGACCCA
    GACTCCAGAA TGAAGACAAC TATGGACCTG ATGGAAGGTT TGTTTCCTCG AGATGTTAAT
    TTGCTGAAGG AAAACAGCGT AAAGAAGAAG GCCGTGCAGA AAACCATCAG CTTCCCAGGA
    TGTGAGACCA AGAGGAGTGA CGACAAGGAA GCAGTGGGCA TGTTGGTCAA CTGTCCTGCC
    TACTACAGCG TCTCTGCTCC CAAGGCTGAG CTTCTCAACA AAATCAAAGC TATGCCAGAA
    GAGGTGAACG AGGAAGAGGA GCAGGCAGAC ATCAACGAAA AGAAGGCGGA GCTGATCGGA
    AGCCTCACCC ACAAGCTCGA GACCCTCCAG GAAGCCAAGG GGAGCCTGCT GATGGACATC
    AAGCTCAATA ACGCCCTGGG GGAGGAGGTG GAGGCCTGGA TCAGTGAGCT CTGCAAGCCC
    AACGAGATCG ACAAGTACAA GATGTTCATC GGGGACTTGG ACAAGGTGGT GAACCTGCTC
    CTGTCCCTCT CGGGGCGCCT GGCCCGCGTG GAGAACGTGC TCAGCGGCCT TGGTGAAGAC
    GCCAGTAACG AGGAAAGGAG TTCACTCAAC GAGAAGAGGA AGGTCCTGTC TGCTCAGCAC
    GAGGACGCCC GGGAGCTCAA GGAGAACGTG GATCGGAGGG AGCGCGTGGT GCTGGACATC
    CTGGCCAATT ACCTCTCCGA AGAGCAGCTC CAAGATTACC GGCATTTCGT GAAAATGAAG
    TCGACACTCC TCATCGAGCA GCGAGAGCTG GATGACAAGA TCAAGCTGGG CCAGGAGCAG
    GTCAAGTGCC TGCTAGAGAG CCTTCCCTCG GACTTCGTGC CGAAGGCGGG GGCCCTGGCT
    CTGCCCCCAG GCCTCACCGG CGATGTGACT CCCGTGGGGG GCTGGACTGG CAGTGGTGTT
    TTCCCAGCGG TGACCTCCCC ACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848716.1
    CDS26..6052
    Translation

    Target ORF information:

    RefSeq Version XM_024992948.1
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992948.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGAGGTCG TGAGTGGCTT TTGGGTGGAA ATCGAGTTCT TTCAGCCCTT CACTCTGTTT 
    CTGAATGCCC TCTGTGGCTG GCTGGGTGCT GGGTGCTGGA AGGTGTGGAG ACAGGAGGTC
    ATCCCTGCCT TCTGGATGCA AACGGTGGGA GTGGGCGTGG CCACAGGCAG TGGGCGGGCT
    CTGCGGGAAA CAGGCTGCTC ACCTGTGGAT CCTCCTCTCT CTAAGCAGAT CAAAGAAGGG
    GGCAAAGCGG ACCTGGTGAG CTCCAGACTT CGGGCTGGGG ACGAGGTGGT GAACATCAAC
    GAGGTGGCAC TGAGCAGCTC CCGGAGGGAG GCCGTTTCCT TGGTGAAAGG ATCCTACAAG
    ACTCTGCGGC TGGTGGTGCG CAGAGATGCA CATGCAGACC CGGGCCCTGC AGACACTGGC
    ACCTCTGACT CATTCGGCCC AGAGCACCTC ACCTCTGACC CCCAGCACAG AAAAGTAGCA
    TGGTCAGGAG GGGTTAAACC TCGGCTGAAG CACAGACGCA CTGAACCTGC AGGCCGGCCT
    CATTCTTGGC ACTCGACCAA ACTTGGGGAG AACCAACCCG ATGCCAGCAT GATGCAGATA
    TCTCAGGGCA CGATTGGCAC GCCTTGGCCC CAGAGCTACC ATTCCAGCTC CTCCACCAGT
    GACCTCTCCG GCTATGACCA CGCATATCTG AGGCAGAGCC CTGACCGGTG CAGCTCCCAG
    GGGAGCATGG AGAGCCTGGA GGCCGGCGGG GGATACCCAC CCTGCCACCT TCTCTCCCCG
    GCCAAGTCCA CCAGCAGCAT TGACCAGCTC AGCCACCTCC ACAGCAAGAG AGATTCGGCT
    TACAGCTCCT TCTCCACCAG TTCCAGCATC CTCGAGTATC CACCCCCTGG CGTCTCTGGC
    CGGGAGCGTT CGGGCTCCAT GGACACCACT TCTGCTCGAG GTGGCCTCCT AGAAGGGATG
    AAGCAGGCAG ATATTCGCTA TATTAAGACA GTCTATGACC CCCGGAGGGG CGTCTCAGCA
    GAGTATGAGG TGAACTCTTC AGGCCTGCTT CTCCAAGGTA GGGAGACCCG AGCCTCAACT
    GATGGTCAGG GCTACAATAA ATTCCATAAT GTTCCTCGGG GCAAAGGCGC ACCACCCTCT
    TCCTGGAGCC AGCAGTGCCC TGGTTCCTTA GAGACCACTG TGGACAACTT GCCTCCTAAA
    GTGGGTGCGC CCCTGCCCCC AGCTCGGAGT GACAGTTATG CAGCCTTCCG GCAGCGAGAG
    AGGCCCAGCT CCTGGTCTAG CCTAGATCAG AAACGGTTCT GCCGACCTCC AGCAAACTCT
    GCAGGCACCC TGAAATCTCC CTTCATAGAG GAACAGCTGC ATACTGTGCT AGAGAAGAGC
    CCAGAAAACA GCCCTCCAGT GAAGCCCAAG CACAATTATA CCCAGAAGGC CCAGCCCGGC
    CAACCTCTGC TGCCGACTGG CATCTACCCG GTACCTTCCC TGGAGCCACA CTTCGCCCAG
    GTGCCCCGGC CTTCTGTGAG TGGAAATGGT ACCCTCTACC CTGCCCTGGC CAAGGAGGGT
    GGGTACACAC CTCCTCAGGG AGCGTGCGAC AGAATGGCTG CCCTTGATGA GAATGGGAAC
    CAAAACGGAT CTAGCAGGCC TGGCTTCGCC TTCTGCCAGG CCTTAGAACG GGACTCAGTG
    TCCCTAGTAG AGAGGAAACC TGAAACTTCA GCCAAATGTG TCCCCTATAA AGTCCATTTT
    CCCTCAGTGC CTGAGAACGA GGAGGAGACC TCCCTGAAGA GACTTCTCAC ACCTCTAGAA
    GGCCACAGCC CACATCCCAG TGAGAGAAAG AGCACCCAGG GCAACAGATA TTCTAATTAC
    CACAGCCTCC AATCCCCTCA GGCTCAGGTG TGCGAAGTGG GTGAAGACAA GAGATCCTTC
    CTGCCTTCAG AGCCTTGGGA GGGGGATTCC CATGAAGACC ACAATGCCAA CCTCCGGGGG
    AGGCTCCACG GAGGCAGCCT GGGCCAGAGT ATGCCAGGCA GCTTTGGCAA GACTGCAGCT
    GCCTTCTCCT CCCTCCAGAA CATTCCTGAG AGTCTAAGAA GGCAAAGCAG CTTGGATCTC
    GGAGCAGCAG CCCAGGAAGT CTACCTGGAA GGCTCATCCA CCTGCGCAGT CAATACCAAG
    GGAGAAGAGT CTGGAAGGAC TGCTGTTGCT GATCACAAGA GCCAGCTGGA CAGGTTGGTG
    TCCTATCCAA GGCCCGAGGG GAGAACCTTG GCCTCTTTCC AGAGTTCAGA CCCAACGTAT
    GAAGAGCCGC CCTCCCCATC CCCACAGGAG ACCTCCAGTC TGGGCCGGAG GAGGCTGAGT
    TCCAGCAGCA CCTCGGCTCT GCAGGGCTTT CAGTACGGGA AGCCCCACTG CTCCGTGCTG
    GAGAAGGTTT CCAAGATCGA GCAGCGAGAG CAGGCTGGCC AGAGACCCCC GAGTGCCGGC
    GGCTCTACTT ATGGCTATAA TTACAGGCCC CACAGGACAC TCCCAGCCTC CAATACTTTG
    GGGAATGACT CGGAGGAGAC TAAAGGCCGT ATACATTTTT CCGAACTCAC TGAACCCCTA
    GGCAATGGAG AACAGCACTT CAAAAACGGG GAGCCAAAGT CAGAAGAGGT TTCCTGGCAG
    CCGTGTGGTC AACAGCCGAG GCGGGCTGTG GAGGGTGGCC GGGGTGCCCC ACTCCGGGGT
    GGAGAGCCCC CGAGGCGGGA CGCCCGTCTG CTCCGCAGCC AGAGCAGCTT CCAGCTCTTC
    AGCGAGGCCG AGAAGGAGGC CATGTGGTCA GACGACAGGC CCCGCACGCC AGAGTCGACT
    GTGCAGGAGG CCCCCTTCAG CCGCGCCTAC CGGAACAGCA TCAAGGATGC ACAGTCCCGC
    GTCCTGGGAG CCACGTCCTT CCGACGCCGG GACCTCGAGC CTGGGACGTC TGTGGCCTCC
    AGGCCCTGGC GCCTGCGACC CGCCTCGGCC CACGTAGGGC TGCGCAGCCC GGAGGCACCG
    ATTTCCGCCT CCCCGCACAC CCCGCGCGAG CGGCACAGCG TGACCCCGGC CGAGGGGGAC
    CCTGCCCGCC TCGCGCCCCC TGCTGCCCGG AGGGGCCCGC GCCGGCATCT GACCACCGAG
    CAGAAGAAGC GCTCGTACTC AGAGCCCGAG AAGATGCACG AGGTGGGGGT CTCGGAGGAA
    CCCGAGCCCG AGCCCCCTGG GCCGCCGAGG AAGGGGCCGC ATTTCATGGA GGGCTCAGTG
    ACTGACCGGC GCCGCATCTT CGAGCGAGAC GGCAGGGCCT GCTCTACCCT CAGCCTGTCG
    GGGCCTGAGC TGAAGCAGTT CCAGCAGAGC GCACTGGCCG ACTACATTCA GCGCAAGACC
    GGCAAGCGGC CTGCGGGCGC CGGCTGTGGC CTGCAGGAGC CGGGGCGGCT GCTGGAGCGC
    ACCCAGAGCA CCTACCTCCA GCCCGAGGGC CCGGGCCTCG CCGCGGCTTC CAGTCTCTGC
    TCGCTTCGGG AGCCCAGCCT GCCGCCCCGC AGGGAGACCG CCCTTCTGCC AGCCACTGTG
    GTGGGGAGTA CTGGGGAGAC CCAGCGGGGC CCCCGAGATC GCAGCAGCTC GTTTGCCAGC
    GGCCGCCACC TTGGGGAAAG GCACCGCGGG GACTCAGCGC CGAGAGAACT GCTCAGTGGA
    GCTAACAATG GATCAAAGGG CCCCCAGAGA CTGAACGGTA CCCCAGGGGA ACCCTCCCCG
    TGGGGGGCCA CAGCTGGGAG GGCCGCGAAG TCCATGTCGG CTGAGGACCT GCTGGAGCGC
    TCAGACGTCC AGGCTGTCCC TGTCCACATG AGGTCCCGGT CATCTCCCAC CGCCGACAAG
    CGCCAGGATG TGCTTTTGGG GGAAAACAGT AGCTTTGGTC TCATGAAGGA TCCGTGTTAT
    TTGACTGGCC CAGGTTCCAG GCCATTCAGC TGTTCCAGCC GTGAAGAGAT GCCAGCGCTC
    AGGCACCACC CCAGCCCTCA CTGGGGGGAT TCGGGCTGCA AAGCAGGTAG TGGTGGTGCC
    TCTGTTCCCA GCGGGGGTCC GGGGCCGCTG GACCCTCCGA GACAGGCCAG CAGAACGCCT
    TGCCCTCTGC CACTGTCCTC GGGAGCTCAT GGGCATCTTC CAGACGCCAG GGCTGCACCC
    CTGAGCTCGG TCTTGTCTGC CCCTGGCCCT TCGAGCTATT GCTCACAGGC TGCAGCCCAG
    CCCTCCACAC CAGCCGGCAC CCCCAGAAGC GGCAGTTGCC ACAGCCCGGC CCAGCCTCCC
    AGCCCTGAGC GCAAGACAGA AGAGCTCGCA GGGATGCGGG CCTCCCCGCC CTGGATGAAG
    CGGACCTACA TTGTCAGAGA GGAGAGCCTC CCCGAGGACC CCCCGAAGCA CTACCCCAAG
    CCACCGGATG CCCCCAGCTC AAGCAGCACT TCTGACCCGG ATACGCCTCT CGGGGCCCCA
    GGCATTCTGG GCAGGATTTC CCTCCGGATC TCCGAGTCGG CCTTGCTGGC TTCCCCGCCA
    CCTCGCGAGG ACTGCGAGGA CGACGAGGTG TTTGTGAGGG ACCCACGCCC CACCGCCACT
    TCCAGCCCCC GCTGCGATGA GCTGCTGCTG CCCCCTCCGC CTCCGCCCCC ACCACCAACA
    ACCAGCCAGG CATCCCTGGC CCAGGGCCTG GACCACTTCC CTCCGCCTCC TCCCGAAGCT
    ATGTGCCCGG CCCAGTGGGA GGAGGGCTAC CTGGAGCCCC GTGCCAGCTC CAGCAAACTT
    GCCAAGGTGA CAGTTGCCAA GGAAAGGTCC ATGGCCGGTG CAGCCCATCC GGTTGATAAT
    CAGCTGCTGG GTTCCAGATC GCAAACTTCA GGCAAGAGTT TCGAGGCCAA GGAGACCAAC
    CCACCCTCCA CCACGGGTGC TCTGCCCCAG CCTGCTGGGT CTCTAAGCAA GCAGCCAAGC
    CCAGGACAGC CACCTCCCAT GCAGACCCAC AGCCTCATCC ACGAGCCAGC CATTGGGACT
    GCAGGTTTAG AAAAGAATGT CAGTTCTGGT CCTCAAAAGA CCTCAGAAGA CATCAGAACA
    GAGACGCTAG CCAAGGAAAT TATCCACCAA GACAAATCTC TGGCAGACAT TTTGGACCCA
    GACTCCAGAA TGAAGACAAC TATGGACCTG ATGGAAGGTT TGTTTCCTCG AGATGTTAAT
    TTGCTGAAGG AAAACAGCGT AAAGAAGAAG GCCGTGCAGA AAACCATCAG CTTCCCAGGA
    TGTGAGACCA AGAGGAGTGA CGACAAGGAA GCAGTGGGCA TGTTGGTCAA CTGTCCTGCC
    TACTACAGCG TCTCTGCTCC CAAGGCTGAG CTTCTCAACA AAATCAAAGC TATGCCAGAA
    GAGGTGAACG AGGAAGAGGA GCAGGCAGAC ATCAACGAAA AGAAGGCGGA GCTGATCGGA
    AGCCTCACCC ACAAGCTCGA GACCCTCCAG GAAGCCAAGG GGAGCCTGCT GATGGACATC
    AAGCTCAATA ACGCCCTGGG GGAGGAGGTG GAGGCCTGGA TCAGTGAGCT CTGCAAGCCC
    AACGAGATCG ACAAGTACAA GATGTTCATC GGGGACTTGG ACAAGGTGGT GAACCTGCTC
    CTGTCCCTCT CGGGGCGCCT GGCCCGCGTG GAGAACGTGC TCAGCGGCCT TGGTGAAGAC
    GCCAGTAACG AGGAAAGGAG TTCACTCAAC GAGAAGAGGA AGGTCCTGTC TGCTCAGCAC
    GAGGACGCCC GGGAGCTCAA GGAGAACGTG GATCGGAGGG AGCGCGTGGT GCTGGACATC
    CTGGCCAATT ACCTCTCCGA AGAGCAGCTC CAAGATTACC GGCATTTCGT GAAAATGAAG
    TCGACACTCC TCATCGAGCA GCGAGAGCTG GATGACAAGA TCAAGCTGGG CCAGGAGCAG
    GTCAAGTGCC TGCTAGAGAG CCTTCCCTCG GACTTCGTGC CGAAGGCGGG GGCCCTGGCT
    CTGCCCCCAG GCCTCACCGG CGATGTGACT CCCGTGGGGG GCTGGACTGG CAGTGGTGTT
    TTCCCAGCGG TGACCTCCCC ACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38287
    Clone ID Related Accession (Same CDS sequence) NM_001191524.1
    Accession Version NM_001191524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product protein Shroom3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02018136.1, DAAA02018137.1 and DAAA02018141.1. On Apr 20, 2011 this sequence version replaced XM_002704629.1. Sequence Note: This RefSeq record was created from genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on full-length homologous protein alignment with human NP_065910.3. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822095 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGATGAGGA CTACGGAGAA CTTCGATGAG ACCAGTGGCA CACTAACCCC TAACAGGACC 
    TCCAGGGGAC GGTACATTTA TCTGGAAGCG TTCCTGCAGG GAGGAGCTCC CTGGGGTTTT
    ACTTTGAAAG GTGGTTTGGA GCATGGGGAA CCATTAATCA TCTCTAAGAT CAAAGAAGGG
    GGCAAAGCGG ACCTGGTGAG CTCCAGACTT CGGGCTGGGG ACGAGGTGGT GAACATCAAC
    GAGGTGGCAC TGAGCAGCTC CCGGAGGGAG GCCGTTTCCT TGGTGAAAGG ATCCTACAAG
    ACTCTGCGGC TGGTGGTGCG CAGAGATGCA CATGCAGACC CGGGCCCTGC AGACACTGGC
    ACCTCTGACT CATTCGGCCC AGAGCACCTC ACCTCTGACC CCCAGCACAG AAAAGTAGCA
    TGGTCAGGAG GGGTTAAACC TCGGCTGAAG CACAGACGCA CTGAACCTGC AGGCCGGCCT
    CATTCTTGGC ACTCGACCAA ACTTGGGGAG AACCAACCCG ATGCCAGCAT GATGCAGATA
    TCTCAGGGCA CGATTGGCAC GCCTTGGCCC CAGAGCTACC ATTCCAGCTC CTCCACCAGT
    GACCTCTCCG GCTATGACCA CGCATATCTG AGGCAGAGCC CTGACCGGTG CAGCTCCCAG
    GGGAGCATGG AGAGCCTGGA GGCCGGCGGG GGATACCCAC CCTGCCACCT TCTCTCCCCG
    GCCAAGTCCA CCAGCAGCAT TGACCAGCTC AGCCACCTCC ACAGCAAGAG AGATTCGGCT
    TACAGCTCCT TCTCCACCAG TTCCAGCATC CTCGAGTATC CACCCCCTGG CGTCTCTGGC
    CGGGAGCGTT CGGGCTCCAT GGACACCACT TCTGCTCGAG GTGGCCTCCT AGAAGGGATG
    AAGCAGGCAG ATATTCGCTA TATTAAGACA GTCTATGACC CCCGGAGGGG CGTCTCAGCA
    GAGTATGAGG TGAACTCTTC AGGCCTGCTT CTCCAAGGTA GGGAGACCCG AGCCTCAACT
    GATGGTCAGG GCTACAATAA ATTCCATAAT GTTCCTCGGG GCAAAGGCGC ACCACCCTCT
    TCCTGGAGCC AGCAGTGCCC TGGTTCCTTA GAGACCACTG TGGACAACTT GCCTCCTAAA
    GTGGGTGCGC CCCTGCCCCC AGCTCGGAGT GACAGTTATG CAGCCTTCCG GCAGCGAGAG
    AGGCCCAGCT CCTGGTCTAG CCTAGATCAG AAACGGTTCT GCCGACCTCC AGCAAACTCT
    GCAGGCACCC TGAAATCTCC CTTCATAGAG GAACAGCTGC ATACTGTGCT AGAGAAGAGC
    CCAGAAAACA GCCCTCCAGT GAAGCCCAAG CACAATTATA CCCAGAAGGC CCAGCCCGGC
    CAACCTCTGC TGCCGACTGG CATCTACCCG GTACCTTCCC TGGAGCCACA CTTCGCCCAG
    GTGCCCCGGC CTTCTGTGAG TGGAAATGGT ACCCTCTACC CTGCCCTGGC CAAGGAGGGT
    GGGTACACAC CTCCTCAGGG AGCGTGCGAC AGAATGGCTG CCCTTGATGA GAATGGGAAC
    CAAAACGGAT CTAGCAGGCC TGGCTTCGCC TTCTGCCAGG CCTTAGAACG GGACTCAGTG
    TCCCTAGTAG AGAGGAAACC TGAAACTTCA GCCAAATGTG TCCCCTATAA AGTCCATTTT
    CCCTCAGTGC CTGAGAACGA GGAGGAGACC TCCCTGAAGA GACTTCTCAC ACCTCTAGAA
    GGCCACAGCC CACATCCCAG TGAGAGAAAG AGCACCCAGG GCAACAGATA TTCTAATTAC
    CACAGCCTCC AATCCCCTCA GGCTCAGGTG TGCGAAGTGG GTGAAGACAA GAGATCCTTC
    CTGCCTTCAG AGCCTTGGGA GGGGGATTCC CATGAAGACC ACAATGCCAA CCTCCGGGGG
    AGGCTCCACG GAGGCAGCCT GGGCCAGAGT ATGCCAGGCA GCTTTGGCAA GACTGCAGCT
    GCCTTCTCCT CCCTCCAGAA CATTCCTGAG AGTCTAAGAA GGCAAAGCAG CTTGGATCTC
    GGAGCAGCAG CCCAGGAAGT CTACCTGGAA GGCTCATCCA CCTGCGCAGT CAATACCAAG
    GGAGAAGAGT CTGGAAGGAC TGCTGTTGCT GATCACAAGA GCCAGCTGGA CAGGTTGGTG
    TCCTATCCAA GGCCCGAGGG GAGAACCTTG GCCTCTTTCC AGAGTTCAGA CCCAACGTAT
    GAAGAGCCGC CCTCCCCATC CCCACAGGAG ACCTCCAGTC TGGGCCGGAG GAGGCTGAGT
    TCCAGCAGCA CCTCGGCTCT GCAGGGCTTT CAGTACGGGA AGCCCCACTG CTCCGTGCTG
    GAGAAGGTTT CCAAGATCGA GCAGCGAGAG CAGGCTGGCC AGAGACCCCC GAGTGCCGGC
    GGCTCTACTT ATGGCTATAA TTACAGGCCC CACAGGACAC TCCCAGCCTC CAATACTTTG
    GGGAATGACT CGGAGGAGAC TAAAGGCCGT ATACATTTTT CCGAACTCAC TGAACCCCTA
    GGCAATGGAG AACAGCACTT CAAAAACGGG GAGCCAAAGT CAGAAGAGGT TTCCTGGCAG
    CCGTGTGGTC AACAGCCGAG GCGGGCTGTG GAGGGTGGCC GGGGTGCCCC ACTCCGGGGT
    GGAGAGCCCC CGAGGCGGGA CGCCCGTCTG CTCCGCAGCC AGAGCAGCTT CCAGCTCTTC
    AGCGAGGCCG AGAAGGAGGC CATGTGGTCA GACGACAGGC CCCGCACGCC AGAGTCGACT
    GTGCAGGAGG CCCCCTTCAG CCGCGCCTAC CGGAACAGCA TCAAGGATGC ACAGTCCCGC
    GTCCTGGGAG CCACGTCCTT CCGACGCCGG GACCTCGAGC CTGGGACGTC TGTGGCCTCC
    AGGCCCTGGC GCCTGCGACC CGCCTCGGCC CACGTAGGGC TGCGCAGCCC GGAGGCACCG
    ATTTCCGCCT CCCCGCACAC CCCGCGCGAG CGGCACAGCG TGACCCCGGC CGAGGGGGAC
    CCTGCCCGCC TCGCGCCCCC TGCTGCCCGG AGGGGCCCGC GCCGGCATCT GACCACCGAG
    CAGAAGAAGC GCTCGTACTC AGAGCCCGAG AAGATGCACG AGGTGGGGGT CTCGGAGGAA
    CCCGAGCCCG AGCCCCCTGG GCCGCCGAGG AAGGGGCCGC ATTTCATGGA GGGCTCAGTG
    ACTGACCGGC GCCGCATCTT CGAGCGAGAC GGCAGGGCCT GCTCTACCCT CAGCCTGTCG
    GGGCCTGAGC TGAAGCAGTT CCAGCAGAGC GCACTGGCCG ACTACATTCA GCGCAAGACC
    GGCAAGCGGC CTGCGGGCGC CGGCTGTGGC CTGCAGGAGC CGGGGCGGCT GCTGGAGCGC
    ACCCAGAGCA CCTACCTCCA GCCCGAGGGC CCGGGCCTCG CCGCGGCTTC CAGTCTCTGC
    TCGCTTCGGG AGCCCAGCCT GCCGCCCCGC AGGGAGACCG CCCTTCTGCC AGCCACTGTG
    GTGGGGAGTA CTGGGGAGAC CCAGCGGGGC CCCCGAGATC GCAGCAGCTC GTTTGCCAGC
    GGCCGCCACC TTGGGGAAAG GCACCGCGGG GACTCAGCGC CGAGAGAACT GCTCAGTGGA
    GCTAACAATG GATCAAAGGG CCCCCAGAGA CTGAACGGTA CCCCAGGGGA ACCCTCCCCG
    TGGGGGGCCA CAGCTGGGAG GGCCGCGAAG TCCATGTCGG CTGAGGACCT GCTGGAGCGC
    TCAGACGTCC AGGCTGTCCC TGTCCACATG AGGTCCCGGT CATCTCCCAC CGCCGACAAG
    CGCCAGGATG TGCTTTTGGG GGAAAACAGT AGCTTTGGTC TCATGAAGGA TCCGTGTTAT
    TTGACTGGCC CAGGTTCCAG GCCATTCAGC TGTTCCAGCC GTGAAGAGAT GCCAGCGCTC
    AGGCACCACC CCAGCCCTCA CTGGGGGGAT TCGGGCTGCA AAGCAGGTAG TGGTGGTGCC
    TCTGTTCCCA GCGGGGGTCC GGGGCCGCTG GACCCTCCGA GACAGGCCAG CAGAACGCCT
    TGCCCTCTGC CACTGTCCTC GGGAGCTCAT GGGCATCTTC CAGACGCCAG GGCTGCACCC
    CTGAGCTCGG TCTTGTCTGC CCCTGGCCCT TCGAGCTATT GCTCACAGGC TGCAGCCCAG
    CCCTCCACAC CAGCCGGCAC CCCCAGAAGC GGCAGTTGCC ACAGCCCGGC CCAGCCTCCC
    AGCCCTGAGC GCAAGACAGA AGAGCTCGCA GGGATGCGGG CCTCCCCGCC CTGGATGAAG
    CGGACCTACA TTGTCAGAGA GGAGAGCCTC CCCGAGGACC CCCCGAAGCA CTACCCCAAG
    CCACCGGATG CCCCCAGCTC AAGCAGCACT TCTGACCCGG ATACGCCTCT CGGGGCCCCA
    GGCATTCTGG GCAGGATTTC CCTCCGGATC TCCGAGTCGG CCTTGCTGGC TTCCCCGCCA
    CCTCGCGAGG ACTGCGAGGA CGACGAGGTG TTTGTGAGGG ACCCACGCCC CACCGCCACT
    TCCAGCCCCC GCTGCGATGA GCTGCTGCTG CCCCCTCCGC CTCCGCCCCC ACCACCAACA
    ACCAGCCAGG CATCCCTGGC CCAGGGCCTG GACCACTTCC CTCCGCCTCC TCCCGAAGCT
    ATGTGCCCGG CCCAGTGGGA GGAGGGCTAC CTGGAGCCCC GTGCCAGCTC CAGCAAACTT
    GCCAAGGTGA CAGTTGCCAA GGAAAGGTCC ATGGCCGGTG CAGCCCATCC GGTTGATAAT
    CAGCTGCTGG GTTCCAGATC GCAAACTTCA GGCAAGAGTT TCGAGGCCAA GGAGACCAAC
    CCACCCTCCA CCACGGGTGC TCTGCCCCAG CCTGCTGGGT CTCTAAGCAA GCAGCCAAGC
    CCAGGACAGC CACCTCCCAT GCAGACCCAC AGCCTCATCC ACGAGCCAGC CATTGGGACT
    GCAGGTTTAG AAAAGAATGT CAGTTCTGGT CCTCAAAAGA CCTCAGAAGA CATCAGAACA
    GAGACGCTAG CCAAGGAAAT TATCCACCAA GACAAATCTC TGGCAGACAT TTTGGACCCA
    GACTCCAGAA TGAAGACAAC TATGGACCTG ATGGAAGGTT TGTTTCCTCG AGATGTTAAT
    TTGCTGAAGG AAAACAGCGT AAAGAAGAAG GCCGTGCAGA AAACCATCAG CTTCCCAGGA
    TGTGAGACCA AGAGGAGTGA CGACAAGGAA GCAGTGGGCA TGTTGGTCAA CTGTCCTGCC
    TACTACAGCG TCTCTGCTCC CAAGGCTGAG CTTCTCAACA AAATCAAAGC TATGCCAGAA
    GAGGTGAACG AGGAAGAGGA GCAGGCAGAC ATCAACGAAA AGAAGGCGGA GCTGATCGGA
    AGCCTCACCC ACAAGCTCGA GACCCTCCAG GAAGCCAAGG GGAGCCTGCT GATGGACATC
    AAGCTCAATA ACGCCCTGGG GGAGGAGGTG GAGGCCTGGA TCAGTGAGCT CTGCAAGCCC
    AACGAGATCG ACAAGTACAA GATGTTCATC GGGGACTTGG ACAAGGTGGT GAACCTGCTC
    CTGTCCCTCT CGGGGCGCCT GGCCCGCGTG GAGAACGTGC TCAGCGGCCT TGGTGAAGAC
    GCCAGTAACG AGGAAAGGAG TTCACTCAAC GAGAAGAGGA AGGTCCTGTC TGCTCAGCAC
    GAGGACGCCC GGGAGCTCAA GGAGAACGTG GATCGGAGGG AGCGCGTGGT GCTGGACATC
    CTGGCCAATT ACCTCTCCGA AGAGCAGCTC CAAGATTACC GGCATTTCGT GAAAATGAAG
    TCGACACTCC TCATCGAGCA GCGAGAGCTG GATGACAAGA TCAAGCTGGG CCAGGAGCAG
    GTCAAGTGCC TGCTAGAGAG CCTTCCCTCG GACTTCGTGC CCAAGGCGGG GGCCCTGGCT
    CTGCCCCCAG GCCTCACCGG CGATGTGACT CCCGTGGGGG GCTGGACTGG CAGTGGTGTT
    TTCCCAGCGG TGACCTCCCC ACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178453.1
    CDS1..5967
    Translation

    Target ORF information:

    RefSeq Version NM_001191524.1
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGATGAGGA CTACGGAGAA CTTCGATGAG ACCAGTGGCA CACTAACCCC TAACAGGACC 
    TCCAGGGGAC GGTACATTTA TCTGGAAGCG TTCCTGCAGG GAGGAGCTCC CTGGGGTTTT
    ACTTTGAAAG GTGGTTTGGA GCATGGGGAA CCATTAATCA TCTCTAAGAT CAAAGAAGGG
    GGCAAAGCGG ACCTGGTGAG CTCCAGACTT CGGGCTGGGG ACGAGGTGGT GAACATCAAC
    GAGGTGGCAC TGAGCAGCTC CCGGAGGGAG GCCGTTTCCT TGGTGAAAGG ATCCTACAAG
    ACTCTGCGGC TGGTGGTGCG CAGAGATGCA CATGCAGACC CGGGCCCTGC AGACACTGGC
    ACCTCTGACT CATTCGGCCC AGAGCACCTC ACCTCTGACC CCCAGCACAG AAAAGTAGCA
    TGGTCAGGAG GGGTTAAACC TCGGCTGAAG CACAGACGCA CTGAACCTGC AGGCCGGCCT
    CATTCTTGGC ACTCGACCAA ACTTGGGGAG AACCAACCCG ATGCCAGCAT GATGCAGATA
    TCTCAGGGCA CGATTGGCAC GCCTTGGCCC CAGAGCTACC ATTCCAGCTC CTCCACCAGT
    GACCTCTCCG GCTATGACCA CGCATATCTG AGGCAGAGCC CTGACCGGTG CAGCTCCCAG
    GGGAGCATGG AGAGCCTGGA GGCCGGCGGG GGATACCCAC CCTGCCACCT TCTCTCCCCG
    GCCAAGTCCA CCAGCAGCAT TGACCAGCTC AGCCACCTCC ACAGCAAGAG AGATTCGGCT
    TACAGCTCCT TCTCCACCAG TTCCAGCATC CTCGAGTATC CACCCCCTGG CGTCTCTGGC
    CGGGAGCGTT CGGGCTCCAT GGACACCACT TCTGCTCGAG GTGGCCTCCT AGAAGGGATG
    AAGCAGGCAG ATATTCGCTA TATTAAGACA GTCTATGACC CCCGGAGGGG CGTCTCAGCA
    GAGTATGAGG TGAACTCTTC AGGCCTGCTT CTCCAAGGTA GGGAGACCCG AGCCTCAACT
    GATGGTCAGG GCTACAATAA ATTCCATAAT GTTCCTCGGG GCAAAGGCGC ACCACCCTCT
    TCCTGGAGCC AGCAGTGCCC TGGTTCCTTA GAGACCACTG TGGACAACTT GCCTCCTAAA
    GTGGGTGCGC CCCTGCCCCC AGCTCGGAGT GACAGTTATG CAGCCTTCCG GCAGCGAGAG
    AGGCCCAGCT CCTGGTCTAG CCTAGATCAG AAACGGTTCT GCCGACCTCC AGCAAACTCT
    GCAGGCACCC TGAAATCTCC CTTCATAGAG GAACAGCTGC ATACTGTGCT AGAGAAGAGC
    CCAGAAAACA GCCCTCCAGT GAAGCCCAAG CACAATTATA CCCAGAAGGC CCAGCCCGGC
    CAACCTCTGC TGCCGACTGG CATCTACCCG GTACCTTCCC TGGAGCCACA CTTCGCCCAG
    GTGCCCCGGC CTTCTGTGAG TGGAAATGGT ACCCTCTACC CTGCCCTGGC CAAGGAGGGT
    GGGTACACAC CTCCTCAGGG AGCGTGCGAC AGAATGGCTG CCCTTGATGA GAATGGGAAC
    CAAAACGGAT CTAGCAGGCC TGGCTTCGCC TTCTGCCAGG CCTTAGAACG GGACTCAGTG
    TCCCTAGTAG AGAGGAAACC TGAAACTTCA GCCAAATGTG TCCCCTATAA AGTCCATTTT
    CCCTCAGTGC CTGAGAACGA GGAGGAGACC TCCCTGAAGA GACTTCTCAC ACCTCTAGAA
    GGCCACAGCC CACATCCCAG TGAGAGAAAG AGCACCCAGG GCAACAGATA TTCTAATTAC
    CACAGCCTCC AATCCCCTCA GGCTCAGGTG TGCGAAGTGG GTGAAGACAA GAGATCCTTC
    CTGCCTTCAG AGCCTTGGGA GGGGGATTCC CATGAAGACC ACAATGCCAA CCTCCGGGGG
    AGGCTCCACG GAGGCAGCCT GGGCCAGAGT ATGCCAGGCA GCTTTGGCAA GACTGCAGCT
    GCCTTCTCCT CCCTCCAGAA CATTCCTGAG AGTCTAAGAA GGCAAAGCAG CTTGGATCTC
    GGAGCAGCAG CCCAGGAAGT CTACCTGGAA GGCTCATCCA CCTGCGCAGT CAATACCAAG
    GGAGAAGAGT CTGGAAGGAC TGCTGTTGCT GATCACAAGA GCCAGCTGGA CAGGTTGGTG
    TCCTATCCAA GGCCCGAGGG GAGAACCTTG GCCTCTTTCC AGAGTTCAGA CCCAACGTAT
    GAAGAGCCGC CCTCCCCATC CCCACAGGAG ACCTCCAGTC TGGGCCGGAG GAGGCTGAGT
    TCCAGCAGCA CCTCGGCTCT GCAGGGCTTT CAGTACGGGA AGCCCCACTG CTCCGTGCTG
    GAGAAGGTTT CCAAGATCGA GCAGCGAGAG CAGGCTGGCC AGAGACCCCC GAGTGCCGGC
    GGCTCTACTT ATGGCTATAA TTACAGGCCC CACAGGACAC TCCCAGCCTC CAATACTTTG
    GGGAATGACT CGGAGGAGAC TAAAGGCCGT ATACATTTTT CCGAACTCAC TGAACCCCTA
    GGCAATGGAG AACAGCACTT CAAAAACGGG GAGCCAAAGT CAGAAGAGGT TTCCTGGCAG
    CCGTGTGGTC AACAGCCGAG GCGGGCTGTG GAGGGTGGCC GGGGTGCCCC ACTCCGGGGT
    GGAGAGCCCC CGAGGCGGGA CGCCCGTCTG CTCCGCAGCC AGAGCAGCTT CCAGCTCTTC
    AGCGAGGCCG AGAAGGAGGC CATGTGGTCA GACGACAGGC CCCGCACGCC AGAGTCGACT
    GTGCAGGAGG CCCCCTTCAG CCGCGCCTAC CGGAACAGCA TCAAGGATGC ACAGTCCCGC
    GTCCTGGGAG CCACGTCCTT CCGACGCCGG GACCTCGAGC CTGGGACGTC TGTGGCCTCC
    AGGCCCTGGC GCCTGCGACC CGCCTCGGCC CACGTAGGGC TGCGCAGCCC GGAGGCACCG
    ATTTCCGCCT CCCCGCACAC CCCGCGCGAG CGGCACAGCG TGACCCCGGC CGAGGGGGAC
    CCTGCCCGCC TCGCGCCCCC TGCTGCCCGG AGGGGCCCGC GCCGGCATCT GACCACCGAG
    CAGAAGAAGC GCTCGTACTC AGAGCCCGAG AAGATGCACG AGGTGGGGGT CTCGGAGGAA
    CCCGAGCCCG AGCCCCCTGG GCCGCCGAGG AAGGGGCCGC ATTTCATGGA GGGCTCAGTG
    ACTGACCGGC GCCGCATCTT CGAGCGAGAC GGCAGGGCCT GCTCTACCCT CAGCCTGTCG
    GGGCCTGAGC TGAAGCAGTT CCAGCAGAGC GCACTGGCCG ACTACATTCA GCGCAAGACC
    GGCAAGCGGC CTGCGGGCGC CGGCTGTGGC CTGCAGGAGC CGGGGCGGCT GCTGGAGCGC
    ACCCAGAGCA CCTACCTCCA GCCCGAGGGC CCGGGCCTCG CCGCGGCTTC CAGTCTCTGC
    TCGCTTCGGG AGCCCAGCCT GCCGCCCCGC AGGGAGACCG CCCTTCTGCC AGCCACTGTG
    GTGGGGAGTA CTGGGGAGAC CCAGCGGGGC CCCCGAGATC GCAGCAGCTC GTTTGCCAGC
    GGCCGCCACC TTGGGGAAAG GCACCGCGGG GACTCAGCGC CGAGAGAACT GCTCAGTGGA
    GCTAACAATG GATCAAAGGG CCCCCAGAGA CTGAACGGTA CCCCAGGGGA ACCCTCCCCG
    TGGGGGGCCA CAGCTGGGAG GGCCGCGAAG TCCATGTCGG CTGAGGACCT GCTGGAGCGC
    TCAGACGTCC AGGCTGTCCC TGTCCACATG AGGTCCCGGT CATCTCCCAC CGCCGACAAG
    CGCCAGGATG TGCTTTTGGG GGAAAACAGT AGCTTTGGTC TCATGAAGGA TCCGTGTTAT
    TTGACTGGCC CAGGTTCCAG GCCATTCAGC TGTTCCAGCC GTGAAGAGAT GCCAGCGCTC
    AGGCACCACC CCAGCCCTCA CTGGGGGGAT TCGGGCTGCA AAGCAGGTAG TGGTGGTGCC
    TCTGTTCCCA GCGGGGGTCC GGGGCCGCTG GACCCTCCGA GACAGGCCAG CAGAACGCCT
    TGCCCTCTGC CACTGTCCTC GGGAGCTCAT GGGCATCTTC CAGACGCCAG GGCTGCACCC
    CTGAGCTCGG TCTTGTCTGC CCCTGGCCCT TCGAGCTATT GCTCACAGGC TGCAGCCCAG
    CCCTCCACAC CAGCCGGCAC CCCCAGAAGC GGCAGTTGCC ACAGCCCGGC CCAGCCTCCC
    AGCCCTGAGC GCAAGACAGA AGAGCTCGCA GGGATGCGGG CCTCCCCGCC CTGGATGAAG
    CGGACCTACA TTGTCAGAGA GGAGAGCCTC CCCGAGGACC CCCCGAAGCA CTACCCCAAG
    CCACCGGATG CCCCCAGCTC AAGCAGCACT TCTGACCCGG ATACGCCTCT CGGGGCCCCA
    GGCATTCTGG GCAGGATTTC CCTCCGGATC TCCGAGTCGG CCTTGCTGGC TTCCCCGCCA
    CCTCGCGAGG ACTGCGAGGA CGACGAGGTG TTTGTGAGGG ACCCACGCCC CACCGCCACT
    TCCAGCCCCC GCTGCGATGA GCTGCTGCTG CCCCCTCCGC CTCCGCCCCC ACCACCAACA
    ACCAGCCAGG CATCCCTGGC CCAGGGCCTG GACCACTTCC CTCCGCCTCC TCCCGAAGCT
    ATGTGCCCGG CCCAGTGGGA GGAGGGCTAC CTGGAGCCCC GTGCCAGCTC CAGCAAACTT
    GCCAAGGTGA CAGTTGCCAA GGAAAGGTCC ATGGCCGGTG CAGCCCATCC GGTTGATAAT
    CAGCTGCTGG GTTCCAGATC GCAAACTTCA GGCAAGAGTT TCGAGGCCAA GGAGACCAAC
    CCACCCTCCA CCACGGGTGC TCTGCCCCAG CCTGCTGGGT CTCTAAGCAA GCAGCCAAGC
    CCAGGACAGC CACCTCCCAT GCAGACCCAC AGCCTCATCC ACGAGCCAGC CATTGGGACT
    GCAGGTTTAG AAAAGAATGT CAGTTCTGGT CCTCAAAAGA CCTCAGAAGA CATCAGAACA
    GAGACGCTAG CCAAGGAAAT TATCCACCAA GACAAATCTC TGGCAGACAT TTTGGACCCA
    GACTCCAGAA TGAAGACAAC TATGGACCTG ATGGAAGGTT TGTTTCCTCG AGATGTTAAT
    TTGCTGAAGG AAAACAGCGT AAAGAAGAAG GCCGTGCAGA AAACCATCAG CTTCCCAGGA
    TGTGAGACCA AGAGGAGTGA CGACAAGGAA GCAGTGGGCA TGTTGGTCAA CTGTCCTGCC
    TACTACAGCG TCTCTGCTCC CAAGGCTGAG CTTCTCAACA AAATCAAAGC TATGCCAGAA
    GAGGTGAACG AGGAAGAGGA GCAGGCAGAC ATCAACGAAA AGAAGGCGGA GCTGATCGGA
    AGCCTCACCC ACAAGCTCGA GACCCTCCAG GAAGCCAAGG GGAGCCTGCT GATGGACATC
    AAGCTCAATA ACGCCCTGGG GGAGGAGGTG GAGGCCTGGA TCAGTGAGCT CTGCAAGCCC
    AACGAGATCG ACAAGTACAA GATGTTCATC GGGGACTTGG ACAAGGTGGT GAACCTGCTC
    CTGTCCCTCT CGGGGCGCCT GGCCCGCGTG GAGAACGTGC TCAGCGGCCT TGGTGAAGAC
    GCCAGTAACG AGGAAAGGAG TTCACTCAAC GAGAAGAGGA AGGTCCTGTC TGCTCAGCAC
    GAGGACGCCC GGGAGCTCAA GGAGAACGTG GATCGGAGGG AGCGCGTGGT GCTGGACATC
    CTGGCCAATT ACCTCTCCGA AGAGCAGCTC CAAGATTACC GGCATTTCGT GAAAATGAAG
    TCGACACTCC TCATCGAGCA GCGAGAGCTG GATGACAAGA TCAAGCTGGG CCAGGAGCAG
    GTCAAGTGCC TGCTAGAGAG CCTTCCCTCG GACTTCGTGC CCAAGGCGGG GGCCCTGGCT
    CTGCCCCCAG GCCTCACCGG CGATGTGACT CCCGTGGGGG GCTGGACTGG CAGTGGTGTT
    TTCCCAGCGG TGACCTCCCC ACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58951
    Clone ID Related Accession (Same CDS sequence) XM_010806187.2
    Accession Version XM_010806187.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCTTTTT ATCCCACTCC TGAGTATGAC ATGCAGGTGG CCAGAGGATT GTTATCTAGA 
    ATGTTTCTCT TTGCCACAAG ACGCACTGAA CCTGCAGGCC GGCCTCATTC TTGGCACTCG
    ACCAAACTTG GGGAGAACCA ACCCGATGCC AGCATGATGC AGATATCTCA GGGCACGATT
    GGCACGCCTT GGCCCCAGAG CTACCATTCC AGCTCCTCCA CCAGTGACCT CTCCGGCTAT
    GACCACGCAT ATCTGAGGCA GAGCCCTGAC CGGTGCAGCT CCCAGGGGAG CATGGAGAGC
    CTGGAGGCCG GCGGGGGATA CCCACCCTGC CACCTTCTCT CCCCGGCCAA GTCCACCAGC
    AGCATTGACC AGCTCAGCCA CCTCCACAGC AAGAGAGATT CGGCTTACAG CTCCTTCTCC
    ACCAGTTCCA GCATCCTCGA GTATCCACCC CCTGGCGTCT CTGGCCGGGA GCGTTCGGGC
    TCCATGGACA CCACTTCTGC TCGAGGTGGC CTCCTAGAAG GGATGAAGCA GGCAGATATT
    CGCTATATTA AGACAGTCTA TGACCCCCGG AGGGGCGTCT CAGCAGAGTA TGAGGTGAAC
    TCTTCAGGCC TGCTTCTCCA AGGTAGGGAG ACCCGAGCCT CAACTGATGG TCAGGGCTAC
    AATAAATTCC ATAATGTTCC TCGGGGCAAA GGCGCACCAC CCTCTTCCTG GAGCCAGCAG
    TGCCCTGGTT CCTTAGAGAC CACTGTGGAC AACTTGCCTC CTAAAGTGGG TGCGCCCCTG
    CCCCCAGCTC GGAGTGACAG TTATGCAGCC TTCCGGCAGC GAGAGAGGCC CAGCTCCTGG
    TCTAGCCTAG ATCAGAAACG GTTCTGCCGA CCTCCAGCAA ACTCTGCAGG CACCCTGAAA
    TCTCCCTTCA TAGAGGAACA GCTGCATACT GTGCTAGAGA AGAGCCCAGA AAACAGCCCT
    CCAGTGAAGC CCAAGCACAA TTATACCCAG AAGGCCCAGC CCGGCCAACC TCTGCTGCCG
    ACTGGCATCT ACCCGGTACC TTCCCTGGAG CCACACTTCG CCCAGGTGCC CCGGCCTTCT
    GTGAGTGGAA ATGGTACCCT CTACCCTGCC CTGGCCAAGG AGGGTGGGTA CACACCTCCT
    CAGGGAGCGT GCGACAGAAT GGCTGCCCTT GATGAGAATG GGAACCAAAA CGGATCTAGC
    AGGCCTGGCT TCGCCTTCTG CCAGGCCTTA GAACGGGACT CAGTGTCCCT AGTAGAGAGG
    AAACCTGAAA CTTCAGCCAA ATGTGTCCCC TATAAAGTCC ATTTTCCCTC AGTGCCTGAG
    AACGAGGAGG AGACCTCCCT GAAGAGACTT CTCACACCTC TAGAAGGCCA CAGCCCACAT
    CCCAGTGAGA GAAAGAGCAC CCAGGGCAAC AGATATTCTA ATTACCACAG CCTCCAATCC
    CCTCAGGCTC AGGTGTGCGA AGTGGGTGAA GACAAGAGAT CCTTCCTGCC TTCAGAGCCT
    TGGGAGGGGG ATTCCCATGA AGACCACAAT GCCAACCTCC GGGGGAGGCT CCACGGAGGC
    AGCCTGGGCC AGAGTATGCC AGGCAGCTTT GGCAAGACTG CAGCTGCCTT CTCCTCCCTC
    CAGAACATTC CTGAGAGTCT AAGAAGGCAA AGCAGCTTGG ATCTCGGAGC AGCAGCCCAG
    GAAGTCTACC TGGAAGGCTC ATCCACCTGC GCAGTCAATA CCAAGGGAGA AGAGTCTGGA
    AGGACTGCTG TTGCTGATCA CAAGAGCCAG CTGGACAGGT TGGTGTCCTA TCCAAGGCCC
    GAGGGGAGAA CCTTGGCCTC TTTCCAGAGT TCAGACCCAA CGTATGAAGA GCCGCCCTCC
    CCATCCCCAC AGGAGACCTC CAGTCTGGGC CGGAGGAGGC TGAGTTCCAG CAGCACCTCG
    GCTCTGCAGG GCTTTCAGTA CGGGAAGCCC CACTGCTCCG TGCTGGAGAA GGTTTCCAAG
    ATCGAGCAGC GAGAGCAGGC TGGCCAGAGA CCCCCGAGTG CCGGCGGCTC TACTTATGGC
    TATAATTACA GGCCCCACAG GACACTCCCA GCCTCCAATA CTTTGGGGAA TGACTCGGAG
    GAGACTAAAG GCCGTATACA TTTTTCCGAA CTCACTGAAC CCCTAGGCAA TGGAGAACAG
    CACTTCAAAA ACGGGGAGCC AAAGTCAGAA GAGGTTTCCT GGCAGCCGTG TGGTCAACAG
    CCGAGGCGGG CTGTGGAGGG TGGCCGGGGT GCCCCACTCC GGGGTGGAGA GCCCCCGAGG
    CGGGACGCCC GTCTGCTCCG CAGCCAGAGC AGCTTCCAGC TCTTCAGCGA GGCCGAGAAG
    GAGGCCATGT GGTCAGACGA CAGGCCCCGC ACGCCAGAGT CGACTGTGCA GGAGGCCCCC
    TTCAGCCGCG CCTACCGGAA CAGCATCAAG GATGCACAGT CCCGCGTCCT GGGAGCCACG
    TCCTTCCGAC GCCGGGACCT CGAGCCTGGG ACGTCTGTGG CCTCCAGGCC CTGGCGCCTG
    CGACCCGCCT CGGCCCACGT AGGGCTGCGC AGCCCGGAGG CACCGATTTC CGCCTCCCCG
    CACACCCCGC GCGAGCGGCA CAGCGTGACC CCGGCCGAGG GGGACCCTGC CCGCCTCGCG
    CCCCCTGCTG CCCGGAGGGG CCCGCGCCGG CATCTGACCA CCGAGCAGAA GAAGCGCTCG
    TACTCAGAGC CCGAGAAGAT GCACGAGGTG GGGGTCTCGG AGGAACCCGA GCCCGAGCCC
    CCTGGGCCGC CGAGGAAGGG GCCGCATTTC ATGGAGGGCT CAGTGACTGA CCGGCGCCGC
    ATCTTCGAGC GAGACGGCAG GGCCTGCTCT ACCCTCAGCC TGTCGGGGCC TGAGCTGAAG
    CAGTTCCAGC AGAGCGCACT GGCCGACTAC ATTCAGCGCA AGACCGGCAA GCGGCCTGCG
    GGCGCCGGCT GTGGCCTGCA GGAGCCGGGG CGGCTGCTGG AGCGCACCCA GAGCACCTAC
    CTCCAGCCCG AGGGCCCGGG CCTCGCCGCG GCTTCCAGTC TCTGCTCGCT TCGGGAGCCC
    AGCCTGCCGC CCCGCAGGGA GACCGCCCTT CTGCCAGCCA CTGTGGTGGG GAGTACTGGG
    GAGACCCAGC GGGGCCCCCG AGATCGCAGC AGCTCGTTTG CCAGCGGCCG CCACCTTGGG
    GAAAGGCACC GCGGGGACTC AGCGCCGAGA GAACTGCTCA GTGGAGCTAA CAATGGATCA
    AAGGGCCCCC AGAGACTGAA CGGTACCCCA GGGGAACCCT CCCCGTGGGG GGCCACAGCT
    GGGAGGGCCG CGAAGTCCAT GTCGGCTGAG GACCTGCTGG AGCGCTCAGA CGTCCAGGCT
    GTCCCTGTCC ACATGAGGTC CCGGTCATCT CCCACCGCCG ACAAGCGCCA GGATGTGCTT
    TTGGGGGAAA ACAGTAGCTT TGGTCTCATG AAGGATCCGT GTTATTTGAC TGGCCCAGGT
    TCCAGGCCAT TCAGCTGTTC CAGCCGTGAA GAGATGCCAG CGCTCAGGCA CCACCCCAGC
    CCTCACTGGG GGGATTCGGG CTGCAAAGCA GGTAGTGGTG GTGCCTCTGT TCCCAGCGGG
    GGTCCGGGGC CGCTGGACCC TCCGAGACAG GCCAGCAGAA CGCCTTGCCC TCTGCCACTG
    TCCTCGGGAG CTCATGGGCA TCTTCCAGAC GCCAGGGCTG CACCCCTGAG CTCGGTCTTG
    TCTGCCCCTG GCCCTTCGAG CTATTGCTCA CAGGCTGCAG CCCAGCCCTC CACACCAGCC
    GGCACCCCCA GAAGCGGCAG TTGCCACAGC CCGGCCCAGC CTCCCAGCCC TGAGCGCAAG
    ACAGAAGAGC TCGCAGGGAT GCGGGCCTCC CCGCCCTGGA TGAAGCGGAC CTACATTGTC
    AGAGAGGAGA GCCTCCCCGA GGACCCCCCG AAGCACTACC CCAAGCCACC GGATGCCCCC
    AGCTCAAGCA GCACTTCTGA CCCGGATACG CCTCTCGGGG CCCCAGGCAT TCTGGGCAGG
    ATTTCCCTCC GGATCTCCGA GTCGGCCTTG CTGGCTTCCC CGCCACCTCG CGAGGACTGC
    GAGGACGACG AGGTGTTTGT GAGGGACCCA CGCCCCACCG CCACTTCCAG CCCCCGCTGC
    GATGAGCTGC TGCTGCCCCC TCCGCCTCCG CCCCCACCAC CAACAACCAG CCAGGCATCC
    CTGGCCCAGG GCCTGGACCA CTTCCCTCCG CCTCCTCCCG AAGCTATGTG CCCGGCCCAG
    TGGGAGGAGG GCTACCTGGA GCCCCGTGCC AGCTCCAGCA AACTTGCCAA GGTGACAGTT
    GCCAAGGAAA GGTCCATGGC CGGTGCAGCC CATCCGGTTG ATAATCAGCT GCTGGGTTCC
    AGATCGCAAA CTTCAGGCAA GAGTTTCGAG GCCAAGGAGA CCAACCCACC CTCCACCACG
    GGTGCTCTGC CCCAGCCTGC TGGGTCTCTA AGCAAGCAGC CAAGCCCAGG ACAGCCACCT
    CCCATGCAGA CCCACAGCCT CATCCACGAG CCAGCCATTG GGACTGCAGG TTTAGAAAAG
    AATGTCAGTT CTGGTCCTCA AAAGACCTCA GAAGACATCA GAACAGAGAC GCTAGCCAAG
    GAAATTATCC ACCAAGACAA ATCTCTGGCA GACATTTTGG ACCCAGACTC CAGAATGAAG
    ACAACTATGG ACCTGATGGA AGGTTTGTTT CCTCGAGATG TTAATTTGCT GAAGGAAAAC
    AGCGTAAAGA AGAAGGCCGT GCAGAAAACC ATCAGCTTCC CAGGATGTGA GACCAAGAGG
    AGTGACGACA AGGAAGCAGT GGGCATGTTG GTCAACTGTC CTGCCTACTA CAGCGTCTCT
    GCTCCCAAGG CTGAGCTTCT CAACAAAATC AAAGCTATGC CAGAAGAGGT GAACGAGGAA
    GAGGAGCAGG CAGACATCAA CGAAAAGAAG GCGGAGCTGA TCGGAAGCCT CACCCACAAG
    CTCGAGACCC TCCAGGAAGC CAAGGGGAGC CTGCTGATGG ACATCAAGCT CAATAACGCC
    CTGGGGGAGG AGGTGGAGGC CTGGATCAGT GAGCTCTGCA AGCCCAACGA GATCGACAAG
    TACAAGATGT TCATCGGGGA CTTGGACAAG GTGGTGAACC TGCTCCTGTC CCTCTCGGGG
    CGCCTGGCCC GCGTGGAGAA CGTGCTCAGC GGCCTTGGTG AAGACGCCAG TAACGAGGAA
    AGGAGTTCAC TCAACGAGAA GAGGAAGGTC CTGTCTGCTC AGCACGAGGA CGCCCGGGAG
    CTCAAGGAGA ACGTGGATCG GAGGGAGCGC GTGGTGCTGG ACATCCTGGC CAATTACCTC
    TCCGAAGAGC AGCTCCAAGA TTACCGGCAT TTCGTGAAAA TGAAGTCGAC ACTCCTCATC
    GAGCAGCGAG AGCTGGATGA CAAGATCAAG CTGGGCCAGG AGCAGGTCAA GTGCCTGCTA
    GAGAGCCTTC CCTCGGACTT CGTGCCCAAG GCGGGGGCCC TGGCTCTGCC CCCAGGCCTC
    ACCGGCGATG TGACTCCCGT GGGGGGCTGG ACTGGCAGTG GTGTTTTCCC AGCGGTGACC
    TCCCCACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804489.1
    CDS134..5725
    Translation

    Target ORF information:

    RefSeq Version XM_010806187.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806187.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGCTTTTT ATCCCACTCC TGAGTATGAC ATGCAGGTGG CCAGAGGATT GTTATCTAGA 
    ATGTTTCTCT TTGCCACAAG ACGCACTGAA CCTGCAGGCC GGCCTCATTC TTGGCACTCG
    ACCAAACTTG GGGAGAACCA ACCCGATGCC AGCATGATGC AGATATCTCA GGGCACGATT
    GGCACGCCTT GGCCCCAGAG CTACCATTCC AGCTCCTCCA CCAGTGACCT CTCCGGCTAT
    GACCACGCAT ATCTGAGGCA GAGCCCTGAC CGGTGCAGCT CCCAGGGGAG CATGGAGAGC
    CTGGAGGCCG GCGGGGGATA CCCACCCTGC CACCTTCTCT CCCCGGCCAA GTCCACCAGC
    AGCATTGACC AGCTCAGCCA CCTCCACAGC AAGAGAGATT CGGCTTACAG CTCCTTCTCC
    ACCAGTTCCA GCATCCTCGA GTATCCACCC CCTGGCGTCT CTGGCCGGGA GCGTTCGGGC
    TCCATGGACA CCACTTCTGC TCGAGGTGGC CTCCTAGAAG GGATGAAGCA GGCAGATATT
    CGCTATATTA AGACAGTCTA TGACCCCCGG AGGGGCGTCT CAGCAGAGTA TGAGGTGAAC
    TCTTCAGGCC TGCTTCTCCA AGGTAGGGAG ACCCGAGCCT CAACTGATGG TCAGGGCTAC
    AATAAATTCC ATAATGTTCC TCGGGGCAAA GGCGCACCAC CCTCTTCCTG GAGCCAGCAG
    TGCCCTGGTT CCTTAGAGAC CACTGTGGAC AACTTGCCTC CTAAAGTGGG TGCGCCCCTG
    CCCCCAGCTC GGAGTGACAG TTATGCAGCC TTCCGGCAGC GAGAGAGGCC CAGCTCCTGG
    TCTAGCCTAG ATCAGAAACG GTTCTGCCGA CCTCCAGCAA ACTCTGCAGG CACCCTGAAA
    TCTCCCTTCA TAGAGGAACA GCTGCATACT GTGCTAGAGA AGAGCCCAGA AAACAGCCCT
    CCAGTGAAGC CCAAGCACAA TTATACCCAG AAGGCCCAGC CCGGCCAACC TCTGCTGCCG
    ACTGGCATCT ACCCGGTACC TTCCCTGGAG CCACACTTCG CCCAGGTGCC CCGGCCTTCT
    GTGAGTGGAA ATGGTACCCT CTACCCTGCC CTGGCCAAGG AGGGTGGGTA CACACCTCCT
    CAGGGAGCGT GCGACAGAAT GGCTGCCCTT GATGAGAATG GGAACCAAAA CGGATCTAGC
    AGGCCTGGCT TCGCCTTCTG CCAGGCCTTA GAACGGGACT CAGTGTCCCT AGTAGAGAGG
    AAACCTGAAA CTTCAGCCAA ATGTGTCCCC TATAAAGTCC ATTTTCCCTC AGTGCCTGAG
    AACGAGGAGG AGACCTCCCT GAAGAGACTT CTCACACCTC TAGAAGGCCA CAGCCCACAT
    CCCAGTGAGA GAAAGAGCAC CCAGGGCAAC AGATATTCTA ATTACCACAG CCTCCAATCC
    CCTCAGGCTC AGGTGTGCGA AGTGGGTGAA GACAAGAGAT CCTTCCTGCC TTCAGAGCCT
    TGGGAGGGGG ATTCCCATGA AGACCACAAT GCCAACCTCC GGGGGAGGCT CCACGGAGGC
    AGCCTGGGCC AGAGTATGCC AGGCAGCTTT GGCAAGACTG CAGCTGCCTT CTCCTCCCTC
    CAGAACATTC CTGAGAGTCT AAGAAGGCAA AGCAGCTTGG ATCTCGGAGC AGCAGCCCAG
    GAAGTCTACC TGGAAGGCTC ATCCACCTGC GCAGTCAATA CCAAGGGAGA AGAGTCTGGA
    AGGACTGCTG TTGCTGATCA CAAGAGCCAG CTGGACAGGT TGGTGTCCTA TCCAAGGCCC
    GAGGGGAGAA CCTTGGCCTC TTTCCAGAGT TCAGACCCAA CGTATGAAGA GCCGCCCTCC
    CCATCCCCAC AGGAGACCTC CAGTCTGGGC CGGAGGAGGC TGAGTTCCAG CAGCACCTCG
    GCTCTGCAGG GCTTTCAGTA CGGGAAGCCC CACTGCTCCG TGCTGGAGAA GGTTTCCAAG
    ATCGAGCAGC GAGAGCAGGC TGGCCAGAGA CCCCCGAGTG CCGGCGGCTC TACTTATGGC
    TATAATTACA GGCCCCACAG GACACTCCCA GCCTCCAATA CTTTGGGGAA TGACTCGGAG
    GAGACTAAAG GCCGTATACA TTTTTCCGAA CTCACTGAAC CCCTAGGCAA TGGAGAACAG
    CACTTCAAAA ACGGGGAGCC AAAGTCAGAA GAGGTTTCCT GGCAGCCGTG TGGTCAACAG
    CCGAGGCGGG CTGTGGAGGG TGGCCGGGGT GCCCCACTCC GGGGTGGAGA GCCCCCGAGG
    CGGGACGCCC GTCTGCTCCG CAGCCAGAGC AGCTTCCAGC TCTTCAGCGA GGCCGAGAAG
    GAGGCCATGT GGTCAGACGA CAGGCCCCGC ACGCCAGAGT CGACTGTGCA GGAGGCCCCC
    TTCAGCCGCG CCTACCGGAA CAGCATCAAG GATGCACAGT CCCGCGTCCT GGGAGCCACG
    TCCTTCCGAC GCCGGGACCT CGAGCCTGGG ACGTCTGTGG CCTCCAGGCC CTGGCGCCTG
    CGACCCGCCT CGGCCCACGT AGGGCTGCGC AGCCCGGAGG CACCGATTTC CGCCTCCCCG
    CACACCCCGC GCGAGCGGCA CAGCGTGACC CCGGCCGAGG GGGACCCTGC CCGCCTCGCG
    CCCCCTGCTG CCCGGAGGGG CCCGCGCCGG CATCTGACCA CCGAGCAGAA GAAGCGCTCG
    TACTCAGAGC CCGAGAAGAT GCACGAGGTG GGGGTCTCGG AGGAACCCGA GCCCGAGCCC
    CCTGGGCCGC CGAGGAAGGG GCCGCATTTC ATGGAGGGCT CAGTGACTGA CCGGCGCCGC
    ATCTTCGAGC GAGACGGCAG GGCCTGCTCT ACCCTCAGCC TGTCGGGGCC TGAGCTGAAG
    CAGTTCCAGC AGAGCGCACT GGCCGACTAC ATTCAGCGCA AGACCGGCAA GCGGCCTGCG
    GGCGCCGGCT GTGGCCTGCA GGAGCCGGGG CGGCTGCTGG AGCGCACCCA GAGCACCTAC
    CTCCAGCCCG AGGGCCCGGG CCTCGCCGCG GCTTCCAGTC TCTGCTCGCT TCGGGAGCCC
    AGCCTGCCGC CCCGCAGGGA GACCGCCCTT CTGCCAGCCA CTGTGGTGGG GAGTACTGGG
    GAGACCCAGC GGGGCCCCCG AGATCGCAGC AGCTCGTTTG CCAGCGGCCG CCACCTTGGG
    GAAAGGCACC GCGGGGACTC AGCGCCGAGA GAACTGCTCA GTGGAGCTAA CAATGGATCA
    AAGGGCCCCC AGAGACTGAA CGGTACCCCA GGGGAACCCT CCCCGTGGGG GGCCACAGCT
    GGGAGGGCCG CGAAGTCCAT GTCGGCTGAG GACCTGCTGG AGCGCTCAGA CGTCCAGGCT
    GTCCCTGTCC ACATGAGGTC CCGGTCATCT CCCACCGCCG ACAAGCGCCA GGATGTGCTT
    TTGGGGGAAA ACAGTAGCTT TGGTCTCATG AAGGATCCGT GTTATTTGAC TGGCCCAGGT
    TCCAGGCCAT TCAGCTGTTC CAGCCGTGAA GAGATGCCAG CGCTCAGGCA CCACCCCAGC
    CCTCACTGGG GGGATTCGGG CTGCAAAGCA GGTAGTGGTG GTGCCTCTGT TCCCAGCGGG
    GGTCCGGGGC CGCTGGACCC TCCGAGACAG GCCAGCAGAA CGCCTTGCCC TCTGCCACTG
    TCCTCGGGAG CTCATGGGCA TCTTCCAGAC GCCAGGGCTG CACCCCTGAG CTCGGTCTTG
    TCTGCCCCTG GCCCTTCGAG CTATTGCTCA CAGGCTGCAG CCCAGCCCTC CACACCAGCC
    GGCACCCCCA GAAGCGGCAG TTGCCACAGC CCGGCCCAGC CTCCCAGCCC TGAGCGCAAG
    ACAGAAGAGC TCGCAGGGAT GCGGGCCTCC CCGCCCTGGA TGAAGCGGAC CTACATTGTC
    AGAGAGGAGA GCCTCCCCGA GGACCCCCCG AAGCACTACC CCAAGCCACC GGATGCCCCC
    AGCTCAAGCA GCACTTCTGA CCCGGATACG CCTCTCGGGG CCCCAGGCAT TCTGGGCAGG
    ATTTCCCTCC GGATCTCCGA GTCGGCCTTG CTGGCTTCCC CGCCACCTCG CGAGGACTGC
    GAGGACGACG AGGTGTTTGT GAGGGACCCA CGCCCCACCG CCACTTCCAG CCCCCGCTGC
    GATGAGCTGC TGCTGCCCCC TCCGCCTCCG CCCCCACCAC CAACAACCAG CCAGGCATCC
    CTGGCCCAGG GCCTGGACCA CTTCCCTCCG CCTCCTCCCG AAGCTATGTG CCCGGCCCAG
    TGGGAGGAGG GCTACCTGGA GCCCCGTGCC AGCTCCAGCA AACTTGCCAA GGTGACAGTT
    GCCAAGGAAA GGTCCATGGC CGGTGCAGCC CATCCGGTTG ATAATCAGCT GCTGGGTTCC
    AGATCGCAAA CTTCAGGCAA GAGTTTCGAG GCCAAGGAGA CCAACCCACC CTCCACCACG
    GGTGCTCTGC CCCAGCCTGC TGGGTCTCTA AGCAAGCAGC CAAGCCCAGG ACAGCCACCT
    CCCATGCAGA CCCACAGCCT CATCCACGAG CCAGCCATTG GGACTGCAGG TTTAGAAAAG
    AATGTCAGTT CTGGTCCTCA AAAGACCTCA GAAGACATCA GAACAGAGAC GCTAGCCAAG
    GAAATTATCC ACCAAGACAA ATCTCTGGCA GACATTTTGG ACCCAGACTC CAGAATGAAG
    ACAACTATGG ACCTGATGGA AGGTTTGTTT CCTCGAGATG TTAATTTGCT GAAGGAAAAC
    AGCGTAAAGA AGAAGGCCGT GCAGAAAACC ATCAGCTTCC CAGGATGTGA GACCAAGAGG
    AGTGACGACA AGGAAGCAGT GGGCATGTTG GTCAACTGTC CTGCCTACTA CAGCGTCTCT
    GCTCCCAAGG CTGAGCTTCT CAACAAAATC AAAGCTATGC CAGAAGAGGT GAACGAGGAA
    GAGGAGCAGG CAGACATCAA CGAAAAGAAG GCGGAGCTGA TCGGAAGCCT CACCCACAAG
    CTCGAGACCC TCCAGGAAGC CAAGGGGAGC CTGCTGATGG ACATCAAGCT CAATAACGCC
    CTGGGGGAGG AGGTGGAGGC CTGGATCAGT GAGCTCTGCA AGCCCAACGA GATCGACAAG
    TACAAGATGT TCATCGGGGA CTTGGACAAG GTGGTGAACC TGCTCCTGTC CCTCTCGGGG
    CGCCTGGCCC GCGTGGAGAA CGTGCTCAGC GGCCTTGGTG AAGACGCCAG TAACGAGGAA
    AGGAGTTCAC TCAACGAGAA GAGGAAGGTC CTGTCTGCTC AGCACGAGGA CGCCCGGGAG
    CTCAAGGAGA ACGTGGATCG GAGGGAGCGC GTGGTGCTGG ACATCCTGGC CAATTACCTC
    TCCGAAGAGC AGCTCCAAGA TTACCGGCAT TTCGTGAAAA TGAAGTCGAC ACTCCTCATC
    GAGCAGCGAG AGCTGGATGA CAAGATCAAG CTGGGCCAGG AGCAGGTCAA GTGCCTGCTA
    GAGAGCCTTC CCTCGGACTT CGTGCCCAAG GCGGGGGCCC TGGCTCTGCC CCCAGGCCTC
    ACCGGCGATG TGACTCCCGT GGGGGGCTGG ACTGGCAGTG GTGTTTTCCC AGCGGTGACC
    TCCCCACTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58952
    Clone ID Related Accession (Same CDS sequence) XM_010806188.2 , XM_010806189.2 , XM_010806190.2
    Accession Version XM_010806188.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCCAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804490.1
    CDS385..5823
    Translation

    Target ORF information:

    RefSeq Version XM_010806188.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806188.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCCAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58952
    Clone ID Related Accession (Same CDS sequence) XM_010806188.2 , XM_010806189.2 , XM_010806190.2
    Accession Version XM_010806189.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806189.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCCAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804491.1
    CDS896..6334
    Translation

    Target ORF information:

    RefSeq Version XM_010806189.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806189.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCCAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58952
    Clone ID Related Accession (Same CDS sequence) XM_010806188.2 , XM_010806189.2 , XM_010806190.2
    Accession Version XM_010806190.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5439bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCCAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804492.1
    CDS563..6001
    Translation

    Target ORF information:

    RefSeq Version XM_010806190.2
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGATGCAGA TATCTCAGGG CACGATTGGC ACGCCTTGGC CCCAGAGCTA CCATTCCAGC 
    TCCTCCACCA GTGACCTCTC CGGCTATGAC CACGCATATC TGAGGCAGAG CCCTGACCGG
    TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG GAGGCCGGCG GGGGATACCC ACCCTGCCAC
    CTTCTCTCCC CGGCCAAGTC CACCAGCAGC ATTGACCAGC TCAGCCACCT CCACAGCAAG
    AGAGATTCGG CTTACAGCTC CTTCTCCACC AGTTCCAGCA TCCTCGAGTA TCCACCCCCT
    GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC ATGGACACCA CTTCTGCTCG AGGTGGCCTC
    CTAGAAGGGA TGAAGCAGGC AGATATTCGC TATATTAAGA CAGTCTATGA CCCCCGGAGG
    GGCGTCTCAG CAGAGTATGA GGTGAACTCT TCAGGCCTGC TTCTCCAAGG TAGGGAGACC
    CGAGCCTCAA CTGATGGTCA GGGCTACAAT AAATTCCATA ATGTTCCTCG GGGCAAAGGC
    GCACCACCCT CTTCCTGGAG CCAGCAGTGC CCTGGTTCCT TAGAGACCAC TGTGGACAAC
    TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC CCAGCTCGGA GTGACAGTTA TGCAGCCTTC
    CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT AGCCTAGATC AGAAACGGTT CTGCCGACCT
    CCAGCAAACT CTGCAGGCAC CCTGAAATCT CCCTTCATAG AGGAACAGCT GCATACTGTG
    CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA GTGAAGCCCA AGCACAATTA TACCCAGAAG
    GCCCAGCCCG GCCAACCTCT GCTGCCGACT GGCATCTACC CGGTACCTTC CCTGGAGCCA
    CACTTCGCCC AGGTGCCCCG GCCTTCTGTG AGTGGAAATG GTACCCTCTA CCCTGCCCTG
    GCCAAGGAGG GTGGGTACAC ACCTCCTCAG GGAGCGTGCG ACAGAATGGC TGCCCTTGAT
    GAGAATGGGA ACCAAAACGG ATCTAGCAGG CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA
    CGGGACTCAG TGTCCCTAGT AGAGAGGAAA CCTGAAACTT CAGCCAAATG TGTCCCCTAT
    AAAGTCCATT TTCCCTCAGT GCCTGAGAAC GAGGAGGAGA CCTCCCTGAA GAGACTTCTC
    ACACCTCTAG AAGGCCACAG CCCACATCCC AGTGAGAGAA AGAGCACCCA GGGCAACAGA
    TATTCTAATT ACCACAGCCT CCAATCCCCT CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC
    AAGAGATCCT TCCTGCCTTC AGAGCCTTGG GAGGGGGATT CCCATGAAGA CCACAATGCC
    AACCTCCGGG GGAGGCTCCA CGGAGGCAGC CTGGGCCAGA GTATGCCAGG CAGCTTTGGC
    AAGACTGCAG CTGCCTTCTC CTCCCTCCAG AACATTCCTG AGAGTCTAAG AAGGCAAAGC
    AGCTTGGATC TCGGAGCAGC AGCCCAGGAA GTCTACCTGG AAGGCTCATC CACCTGCGCA
    GTCAATACCA AGGGAGAAGA GTCTGGAAGG ACTGCTGTTG CTGATCACAA GAGCCAGCTG
    GACAGGTTGG TGTCCTATCC AAGGCCCGAG GGGAGAACCT TGGCCTCTTT CCAGAGTTCA
    GACCCAACGT ATGAAGAGCC GCCCTCCCCA TCCCCACAGG AGACCTCCAG TCTGGGCCGG
    AGGAGGCTGA GTTCCAGCAG CACCTCGGCT CTGCAGGGCT TTCAGTACGG GAAGCCCCAC
    TGCTCCGTGC TGGAGAAGGT TTCCAAGATC GAGCAGCGAG AGCAGGCTGG CCAGAGACCC
    CCGAGTGCCG GCGGCTCTAC TTATGGCTAT AATTACAGGC CCCACAGGAC ACTCCCAGCC
    TCCAATACTT TGGGGAATGA CTCGGAGGAG ACTAAAGGCC GTATACATTT TTCCGAACTC
    ACTGAACCCC TAGGCAATGG AGAACAGCAC TTCAAAAACG GGGAGCCAAA GTCAGAAGAG
    GTTTCCTGGC AGCCGTGTGG TCAACAGCCG AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC
    CCACTCCGGG GTGGAGAGCC CCCGAGGCGG GACGCCCGTC TGCTCCGCAG CCAGAGCAGC
    TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG GCCATGTGGT CAGACGACAG GCCCCGCACG
    CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC AGCCGCGCCT ACCGGAACAG CATCAAGGAT
    GCACAGTCCC GCGTCCTGGG AGCCACGTCC TTCCGACGCC GGGACCTCGA GCCTGGGACG
    TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA CCCGCCTCGG CCCACGTAGG GCTGCGCAGC
    CCGGAGGCAC CGATTTCCGC CTCCCCGCAC ACCCCGCGCG AGCGGCACAG CGTGACCCCG
    GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT
    CTGACCACCG AGCAGAAGAA GCGCTCGTAC TCAGAGCCCG AGAAGATGCA CGAGGTGGGG
    GTCTCGGAGG AACCCGAGCC CGAGCCCCCT GGGCCGCCGA GGAAGGGGCC GCATTTCATG
    GAGGGCTCAG TGACTGACCG GCGCCGCATC TTCGAGCGAG ACGGCAGGGC CTGCTCTACC
    CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG TTCCAGCAGA GCGCACTGGC CGACTACATT
    CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG
    CTGCTGGAGC GCACCCAGAG CACCTACCTC CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT
    TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG
    CCAGCCACTG TGGTGGGGAG TACTGGGGAG ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC
    TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA AGGCACCGCG GGGACTCAGC GCCGAGAGAA
    CTGCTCAGTG GAGCTAACAA TGGATCAAAG GGCCCCCAGA GACTGAACGG TACCCCAGGG
    GAACCCTCCC CGTGGGGGGC CACAGCTGGG AGGGCCGCGA AGTCCATGTC GGCTGAGGAC
    CTGCTGGAGC GCTCAGACGT CCAGGCTGTC CCTGTCCACA TGAGGTCCCG GTCATCTCCC
    ACCGCCGACA AGCGCCAGGA TGTGCTTTTG GGGGAAAACA GTAGCTTTGG TCTCATGAAG
    GATCCGTGTT ATTTGACTGG CCCAGGTTCC AGGCCATTCA GCTGTTCCAG CCGTGAAGAG
    ATGCCAGCGC TCAGGCACCA CCCCAGCCCT CACTGGGGGG ATTCGGGCTG CAAAGCAGGT
    AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT CCGGGGCCGC TGGACCCTCC GAGACAGGCC
    AGCAGAACGC CTTGCCCTCT GCCACTGTCC TCGGGAGCTC ATGGGCATCT TCCAGACGCC
    AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT GCCCCTGGCC CTTCGAGCTA TTGCTCACAG
    GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC ACCCCCAGAA GCGGCAGTTG CCACAGCCCG
    GCCCAGCCTC CCAGCCCTGA GCGCAAGACA GAAGAGCTCG CAGGGATGCG GGCCTCCCCG
    CCCTGGATGA AGCGGACCTA CATTGTCAGA GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG
    CACTACCCCA AGCCACCGGA TGCCCCCAGC TCAAGCAGCA CTTCTGACCC GGATACGCCT
    CTCGGGGCCC CAGGCATTCT GGGCAGGATT TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG
    GCTTCCCCGC CACCTCGCGA GGACTGCGAG GACGACGAGG TGTTTGTGAG GGACCCACGC
    CCCACCGCCA CTTCCAGCCC CCGCTGCGAT GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC
    CCACCACCAA CAACCAGCCA GGCATCCCTG GCCCAGGGCC TGGACCACTT CCCTCCGCCT
    CCTCCCGAAG CTATGTGCCC GGCCCAGTGG GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC
    TCCAGCAAAC TTGCCAAGGT GACAGTTGCC AAGGAAAGGT CCATGGCCGG TGCAGCCCAT
    CCGGTTGATA ATCAGCTGCT GGGTTCCAGA TCGCAAACTT CAGGCAAGAG TTTCGAGGCC
    AAGGAGACCA ACCCACCCTC CACCACGGGT GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC
    AAGCAGCCAA GCCCAGGACA GCCACCTCCC ATGCAGACCC ACAGCCTCAT CCACGAGCCA
    GCCATTGGGA CTGCAGGTTT AGAAAAGAAT GTCAGTTCTG GTCCTCAAAA GACCTCAGAA
    GACATCAGAA CAGAGACGCT AGCCAAGGAA ATTATCCACC AAGACAAATC TCTGGCAGAC
    ATTTTGGACC CAGACTCCAG AATGAAGACA ACTATGGACC TGATGGAAGG TTTGTTTCCT
    CGAGATGTTA ATTTGCTGAA GGAAAACAGC GTAAAGAAGA AGGCCGTGCA GAAAACCATC
    AGCTTCCCAG GATGTGAGAC CAAGAGGAGT GACGACAAGG AAGCAGTGGG CATGTTGGTC
    AACTGTCCTG CCTACTACAG CGTCTCTGCT CCCAAGGCTG AGCTTCTCAA CAAAATCAAA
    GCTATGCCAG AAGAGGTGAA CGAGGAAGAG GAGCAGGCAG ACATCAACGA AAAGAAGGCG
    GAGCTGATCG GAAGCCTCAC CCACAAGCTC GAGACCCTCC AGGAAGCCAA GGGGAGCCTG
    CTGATGGACA TCAAGCTCAA TAACGCCCTG GGGGAGGAGG TGGAGGCCTG GATCAGTGAG
    CTCTGCAAGC CCAACGAGAT CGACAAGTAC AAGATGTTCA TCGGGGACTT GGACAAGGTG
    GTGAACCTGC TCCTGTCCCT CTCGGGGCGC CTGGCCCGCG TGGAGAACGT GCTCAGCGGC
    CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG AGTTCACTCA ACGAGAAGAG GAAGGTCCTG
    TCTGCTCAGC ACGAGGACGC CCGGGAGCTC AAGGAGAACG TGGATCGGAG GGAGCGCGTG
    GTGCTGGACA TCCTGGCCAA TTACCTCTCC GAAGAGCAGC TCCAAGATTA CCGGCATTTC
    GTGAAAATGA AGTCGACACT CCTCATCGAG CAGCGAGAGC TGGATGACAA GATCAAGCTG
    GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG AGCCTTCCCT CGGACTTCGT GCCCAAGGCG
    GGGGCCCTGG CTCTGCCCCC AGGCCTCACC GGCGATGTGA CTCCCGTGGG GGGCTGGACT
    GGCAGTGGTG TTTTCCCAGC GGTGACCTCC CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90945
    Clone ID Related Accession (Same CDS sequence) XM_015471673.1
    Accession Version XM_015471673.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein Shroom3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103980.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGAGCTG AGGCAAGGTG GCGGGGAGGT GCCCCTGTGT GGGGGAGTGC TGTTGTTTTT 
    AGAACCAGTG CCAGCTCTGA GTGGATGGTG AGTAAGAGCC TCTTCCTCTT CCCTCTGGGC
    TGTCCTCCTC TCCCATCCCA ATCTTGTTGC TGGCCTGTTT CGGATATGGT GCTAAGCAAG
    GGCGGCTGCC TGGCTCTGAT GTTCCCCAGG CTTGGGACCC AAAACTCGAG AAGGCGGCAA
    AATCCAGATG CACATGCAGA CCCGGGCCCT GCAGACACTG GCACCTCTGA CTCATTCGGC
    CCAGAGCACC TCACCTCTGA CCCCCAGCAC AGAAAAGTAG CATGGTCAGG AGGGGTTAAA
    CCTCGGCTGA AGCACAGACG CACTGAACCT GCAGGCCGGC CTCATTCTTG GCACTCGACC
    AAACTTGGGG AGAACCAACC CGATGCCAGC ATGATGCAGA TATCTCAGGG CACGATTGGC
    ACGCCTTGGC CCCAGAGCTA CCATTCCAGC TCCTCCACCA GTGACCTCTC CGGCTATGAC
    CACGCATATC TGAGGCAGAG CCCTGACCGG TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG
    GAGGCCGGCG GGGGATACCC ACCCTGCCAC CTTCTCTCCC CGGCCAAGTC CACCAGCAGC
    ATTGACCAGC TCAGCCACCT CCACAGCAAG AGAGATTCGG CTTACAGCTC CTTCTCCACC
    AGTTCCAGCA TCCTCGAGTA TCCACCCCCT GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC
    ATGGACACCA CTTCTGCTCG AGGTGGCCTC CTAGAAGGGA TGAAGCAGGC AGATATTCGC
    TATATTAAGA CAGTCTATGA CCCCCGGAGG GGCGTCTCAG CAGAGTATGA GGTGAACTCT
    TCAGGCCTGC TTCTCCAAGG TAGGGAGACC CGAGCCTCAA CTGATGGTCA GGGCTACAAT
    AAATTCCATA ATGTTCCTCG GGGCAAAGGC GCACCACCCT CTTCCTGGAG CCAGCAGTGC
    CCTGGTTCCT TAGAGACCAC TGTGGACAAC TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC
    CCAGCTCGGA GTGACAGTTA TGCAGCCTTC CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT
    AGCCTAGATC AGAAACGGTT CTGCCGACCT CCAGCAAACT CTGCAGGCAC CCTGAAATCT
    CCCTTCATAG AGGAACAGCT GCATACTGTG CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA
    GTGAAGCCCA AGCACAATTA TACCCAGAAG GCCCAGCCCG GCCAACCTCT GCTGCCGACT
    GGCATCTACC CGGTACCTTC CCTGGAGCCA CACTTCGCCC AGGTGCCCCG GCCTTCTGTG
    AGTGGAAATG GTACCCTCTA CCCTGCCCTG GCCAAGGAGG GTGGGTACAC ACCTCCTCAG
    GGAGCGTGCG ACAGAATGGC TGCCCTTGAT GAGAATGGGA ACCAAAACGG ATCTAGCAGG
    CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA CGGGACTCAG TGTCCCTAGT AGAGAGGAAA
    CCTGAAACTT CAGCCAAATG TGTCCCCTAT AAAGTCCATT TTCCCTCAGT GCCTGAGAAC
    GAGGAGGAGA CCTCCCTGAA GAGACTTCTC ACACCTCTAG AAGGCCACAG CCCACATCCC
    AGTGAGAGAA AGAGCACCCA GGGCAACAGA TATTCTAATT ACCACAGCCT CCAATCCCCT
    CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC AAGAGATCCT TCCTGCCTTC AGAGCCTTGG
    GAGGGGGATT CCCATGAAGA CCACAATGCC AACCTCCGGG GGAGGCTCCA CGGAGGCAGC
    CTGGGCCAGA GTATGCCAGG CAGCTTTGGC AAGACTGCAG CTGCCTTCTC CTCCCTCCAG
    AACATTCCTG AGAGTCTAAG AAGGCAAAGC AGCTTGGATC TCGGAGCAGC AGCCCAGGAA
    GTCTACCTGG AAGGCTCATC CACCTGCGCA GTCAATACCA AGGGAGAAGA GTCTGGAAGG
    ACTGCTGTTG CTGATCACAA GAGCCAGCTG GACAGGTTGG TGTCCTATCC AAGGCCCGAG
    GGGAGAACCT TGGCCTCTTT CCAGAGTTCA GACCCAACGT ATGAAGAGCC GCCCTCCCCA
    TCCCCACAGG AGACCTCCAG TCTGGGCCGG AGGAGGCTGA GTTCCAGCAG CACCTCGGCT
    CTGCAGGGCT TTCAGTACGG GAAGCCCCAC TGCTCCGTGC TGGAGAAGGT TTCCAAGATC
    GAGCAGCGAG AGCAGGCTGG CCAGAGACCC CCGAGTGCCG GCGGCTCTAC TTATGGCTAT
    AATTACAGGC CCCACAGGAC ACTCCCAGCC TCCAATACTT TGGGGAATGA CTCGGAGGAG
    ACTAAAGGCC GTATACATTT TTCCGAACTC ACTGAACCCC TAGGCAATGG AGAACAGCAC
    TTCAAAAACG GGGAGCCAAA GTCAGAAGAG GTTTCCTGGC AGCCGTGTGG TCAACAGCCG
    AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC CCACTCCGGG GTGGAGAGCC CCCGAGGCGG
    GACGCCCGTC TGCTCCGCAG CCAGAGCAGC TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG
    GCCATGTGGT CAGACGACAG GCCCCGCACG CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC
    AGCCGCGCCT ACCGGAACAG CATCAAGGAT GCACAGTCCC GCGTCCTGGG AGCCACGTCC
    TTCCGACGCC GGGACCTCGA GCCTGGGACG TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA
    CCCGCCTCGG CCCACGTAGG GCTGCGCAGC CCGGAGGCAC CGATTTCCGC CTCCCCGCAC
    ACCCCGCGCG AGCGGCACAG CGTGACCCCG GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC
    CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT CTGACCACCG AGCAGAAGAA GCGCTCGTAC
    TCAGAGCCCG AGAAGATGCA CGAGGTGGGG GTCTCGGAGG AACCCGAGCC CGAGCCCCCT
    GGGCCGCCGA GGAAGGGGCC GCATTTCATG GAGGGCTCAG TGACTGACCG GCGCCGCATC
    TTCGAGCGAG ACGGCAGGGC CTGCTCTACC CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG
    TTCCAGCAGA GCGCACTGGC CGACTACATT CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC
    GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG CTGCTGGAGC GCACCCAGAG CACCTACCTC
    CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC
    CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG CCAGCCACTG TGGTGGGGAG TACTGGGGAG
    ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA
    AGGCACCGCG GGGACTCAGC GCCGAGAGAA CTGCTCAGTG GAGCTAACAA TGGATCAAAG
    GGCCCCCAGA GACTGAACGG TACCCCAGGG GAACCCTCCC CGTGGGGGGC CACAGCTGGG
    AGGGCCGCGA AGTCCATGTC GGCTGAGGAC CTGCTGGAGC GCTCAGACGT CCAGGCTGTC
    CCTGTCCACA TGAGGTCCCG GTCATCTCCC ACCGCCGACA AGCGCCAGGA TGTGCTTTTG
    GGGGAAAACA GTAGCTTTGG TCTCATGAAG GATCCGTGTT ATTTGACTGG CCCAGGTTCC
    AGGCCATTCA GCTGTTCCAG CCGTGAAGAG ATGCCAGCGC TCAGGCACCA CCCCAGCCCT
    CACTGGGGGG ATTCGGGCTG CAAAGCAGGT AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT
    CCGGGGCCGC TGGACCCTCC GAGACAGGCC AGCAGAACGC CTTGCCCTCT GCCACTGTCC
    TCGGGAGCTC ATGGGCATCT TCCAGACGCC AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT
    GCCCCTGGCC CTTCGAGCTA TTGCTCACAG GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC
    ACCCCCAGAA GCGGCAGTTG CCACAGCCCG GCCCAGCCTC CCAGCCCTGA GCGCAAGACA
    GAAGAGCTCG CAGGGATGCG GGCCTCCCCG CCCTGGATGA AGCGGACCTA CATTGTCAGA
    GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG CACTACCCCA AGCCACCGGA TGCCCCCAGC
    TCAAGCAGCA CTTCTGACCC GGATACGCCT CTCGGGGCCC CAGGCATTCT GGGCAGGATT
    TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG GCTTCCCCGC CACCTCGCGA GGACTGCGAG
    GACGACGAGG TGTTTGTGAG GGACCCACGC CCCACCGCCA CTTCCAGCCC CCGCTGCGAT
    GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC CCACCACCAA CAACCAGCCA GGCATCCCTG
    GCCCAGGGCC TGGACCACTT CCCTCCGCCT CCTCCCGAAG CTATGTGCCC GGCCCAGTGG
    GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC TCCAGCAAAC TTGCCAAGGT GACAGTTGCC
    AAGGAAAGGT CCATGGCCGG TGCAGCCCAT CCGGTTGATA ATCAGCTGCT GGGTTCCAGA
    TCGCAAACTT CAGGCAAGAG TTTCGAGGCC AAGGAGACCA ACCCACCCTC CACCACGGGT
    GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC AAGCAGCCAA GCCCAGGACA GCCACCTCCC
    ATGCAGACCC ACAGCCTCAT CCACGAGCCA GCCATTGGGA CTGCAGGTTT AGAAAAGAAT
    GTCAGTTCTG GTCCTCAAAA GACCTCAGAA GACATCAGAA CAGAGACGCT AGCCAAGGAA
    ATTATCCACC AAGACAAATC TCTGGCAGAC ATTTTGGACC CAGACTCCAG AATGAAGACA
    ACTATGGACC TGATGGAAGG TTTGTTTCCT CGAGATGTTA ATTTGCTGAA GGAAAACAGC
    GTAAAGAAGA AGGCCGTGCA GAAAACCATC AGCTTCCCAG GATGTGAGAC CAAGAGGAGT
    GACGACAAGG AAGCAGTGGG CATGTTGGTC AACTGTCCTG CCTACTACAG CGTCTCTGCT
    CCCAAGGCTG AGCTTCTCAA CAAAATCAAA GCTATGCCAG AAGAGGTGAA CGAGGAAGAG
    GAGCAGGCAG ACATCAACGA AAAGAAGGCG GAGCTGATCG GAAGCCTCAC CCACAAGCTC
    GAGACCCTCC AGGAAGCCAA GGGGAGCCTG CTGATGGACA TCAAGCTCAA TAACGCCCTG
    GGGGAGGAGG TGGAGGCCTG GATCAGTGAG CTCTGCAAGC CCAACGAGAT CGACAAGTAC
    AAGATGTTCA TCGGGGACTT GGACAAGGTG GTGAACCTGC TCCTGTCCCT CTCGGGGCGC
    CTGGCCCGCG TGGAGAACGT GCTCAGCGGC CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG
    AGTTCACTCA ACGAGAAGAG GAAGGTCCTG TCTGCTCAGC ACGAGGACGC CCGGGAGCTC
    AAGGAGAACG TGGATCGGAG GGAGCGCGTG GTGCTGGACA TCCTGGCCAA TTACCTCTCC
    GAAGAGCAGC TCCAAGATTA CCGGCATTTC GTGAAAATGA AGTCGACACT CCTCATCGAG
    CAGCGAGAGC TGGATGACAA GATCAAGCTG GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG
    AGCCTTCCCT CGGACTTCGT GCCCAAGGCG GGGGCCCTGG CTCTGCCCCC AGGCCTCACC
    GGCGATGTGA CTCCCGTGGG GGGCTGGACT GGCAGTGGTG TTTTCCCAGC GGTGACCTCC
    CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327159.1
    CDS378..6266
    Translation

    Target ORF information:

    RefSeq Version XM_015471673.1
    Organism Bos taurus(cattle)
    Definition Bos taurus shroom family member 3 (SHROOM3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471673.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGAGCTG AGGCAAGGTG GCGGGGAGGT GCCCCTGTGT GGGGGAGTGC TGTTGTTTTT 
    AGAACCAGTG CCAGCTCTGA GTGGATGGTG AGTAAGAGCC TCTTCCTCTT CCCTCTGGGC
    TGTCCTCCTC TCCCATCCCA ATCTTGTTGC TGGCCTGTTT CGGATATGGT GCTAAGCAAG
    GGCGGCTGCC TGGCTCTGAT GTTCCCCAGG CTTGGGACCC AAAACTCGAG AAGGCGGCAA
    AATCCAGATG CACATGCAGA CCCGGGCCCT GCAGACACTG GCACCTCTGA CTCATTCGGC
    CCAGAGCACC TCACCTCTGA CCCCCAGCAC AGAAAAGTAG CATGGTCAGG AGGGGTTAAA
    CCTCGGCTGA AGCACAGACG CACTGAACCT GCAGGCCGGC CTCATTCTTG GCACTCGACC
    AAACTTGGGG AGAACCAACC CGATGCCAGC ATGATGCAGA TATCTCAGGG CACGATTGGC
    ACGCCTTGGC CCCAGAGCTA CCATTCCAGC TCCTCCACCA GTGACCTCTC CGGCTATGAC
    CACGCATATC TGAGGCAGAG CCCTGACCGG TGCAGCTCCC AGGGGAGCAT GGAGAGCCTG
    GAGGCCGGCG GGGGATACCC ACCCTGCCAC CTTCTCTCCC CGGCCAAGTC CACCAGCAGC
    ATTGACCAGC TCAGCCACCT CCACAGCAAG AGAGATTCGG CTTACAGCTC CTTCTCCACC
    AGTTCCAGCA TCCTCGAGTA TCCACCCCCT GGCGTCTCTG GCCGGGAGCG TTCGGGCTCC
    ATGGACACCA CTTCTGCTCG AGGTGGCCTC CTAGAAGGGA TGAAGCAGGC AGATATTCGC
    TATATTAAGA CAGTCTATGA CCCCCGGAGG GGCGTCTCAG CAGAGTATGA GGTGAACTCT
    TCAGGCCTGC TTCTCCAAGG TAGGGAGACC CGAGCCTCAA CTGATGGTCA GGGCTACAAT
    AAATTCCATA ATGTTCCTCG GGGCAAAGGC GCACCACCCT CTTCCTGGAG CCAGCAGTGC
    CCTGGTTCCT TAGAGACCAC TGTGGACAAC TTGCCTCCTA AAGTGGGTGC GCCCCTGCCC
    CCAGCTCGGA GTGACAGTTA TGCAGCCTTC CGGCAGCGAG AGAGGCCCAG CTCCTGGTCT
    AGCCTAGATC AGAAACGGTT CTGCCGACCT CCAGCAAACT CTGCAGGCAC CCTGAAATCT
    CCCTTCATAG AGGAACAGCT GCATACTGTG CTAGAGAAGA GCCCAGAAAA CAGCCCTCCA
    GTGAAGCCCA AGCACAATTA TACCCAGAAG GCCCAGCCCG GCCAACCTCT GCTGCCGACT
    GGCATCTACC CGGTACCTTC CCTGGAGCCA CACTTCGCCC AGGTGCCCCG GCCTTCTGTG
    AGTGGAAATG GTACCCTCTA CCCTGCCCTG GCCAAGGAGG GTGGGTACAC ACCTCCTCAG
    GGAGCGTGCG ACAGAATGGC TGCCCTTGAT GAGAATGGGA ACCAAAACGG ATCTAGCAGG
    CCTGGCTTCG CCTTCTGCCA GGCCTTAGAA CGGGACTCAG TGTCCCTAGT AGAGAGGAAA
    CCTGAAACTT CAGCCAAATG TGTCCCCTAT AAAGTCCATT TTCCCTCAGT GCCTGAGAAC
    GAGGAGGAGA CCTCCCTGAA GAGACTTCTC ACACCTCTAG AAGGCCACAG CCCACATCCC
    AGTGAGAGAA AGAGCACCCA GGGCAACAGA TATTCTAATT ACCACAGCCT CCAATCCCCT
    CAGGCTCAGG TGTGCGAAGT GGGTGAAGAC AAGAGATCCT TCCTGCCTTC AGAGCCTTGG
    GAGGGGGATT CCCATGAAGA CCACAATGCC AACCTCCGGG GGAGGCTCCA CGGAGGCAGC
    CTGGGCCAGA GTATGCCAGG CAGCTTTGGC AAGACTGCAG CTGCCTTCTC CTCCCTCCAG
    AACATTCCTG AGAGTCTAAG AAGGCAAAGC AGCTTGGATC TCGGAGCAGC AGCCCAGGAA
    GTCTACCTGG AAGGCTCATC CACCTGCGCA GTCAATACCA AGGGAGAAGA GTCTGGAAGG
    ACTGCTGTTG CTGATCACAA GAGCCAGCTG GACAGGTTGG TGTCCTATCC AAGGCCCGAG
    GGGAGAACCT TGGCCTCTTT CCAGAGTTCA GACCCAACGT ATGAAGAGCC GCCCTCCCCA
    TCCCCACAGG AGACCTCCAG TCTGGGCCGG AGGAGGCTGA GTTCCAGCAG CACCTCGGCT
    CTGCAGGGCT TTCAGTACGG GAAGCCCCAC TGCTCCGTGC TGGAGAAGGT TTCCAAGATC
    GAGCAGCGAG AGCAGGCTGG CCAGAGACCC CCGAGTGCCG GCGGCTCTAC TTATGGCTAT
    AATTACAGGC CCCACAGGAC ACTCCCAGCC TCCAATACTT TGGGGAATGA CTCGGAGGAG
    ACTAAAGGCC GTATACATTT TTCCGAACTC ACTGAACCCC TAGGCAATGG AGAACAGCAC
    TTCAAAAACG GGGAGCCAAA GTCAGAAGAG GTTTCCTGGC AGCCGTGTGG TCAACAGCCG
    AGGCGGGCTG TGGAGGGTGG CCGGGGTGCC CCACTCCGGG GTGGAGAGCC CCCGAGGCGG
    GACGCCCGTC TGCTCCGCAG CCAGAGCAGC TTCCAGCTCT TCAGCGAGGC CGAGAAGGAG
    GCCATGTGGT CAGACGACAG GCCCCGCACG CCAGAGTCGA CTGTGCAGGA GGCCCCCTTC
    AGCCGCGCCT ACCGGAACAG CATCAAGGAT GCACAGTCCC GCGTCCTGGG AGCCACGTCC
    TTCCGACGCC GGGACCTCGA GCCTGGGACG TCTGTGGCCT CCAGGCCCTG GCGCCTGCGA
    CCCGCCTCGG CCCACGTAGG GCTGCGCAGC CCGGAGGCAC CGATTTCCGC CTCCCCGCAC
    ACCCCGCGCG AGCGGCACAG CGTGACCCCG GCCGAGGGGG ACCCTGCCCG CCTCGCGCCC
    CCTGCTGCCC GGAGGGGCCC GCGCCGGCAT CTGACCACCG AGCAGAAGAA GCGCTCGTAC
    TCAGAGCCCG AGAAGATGCA CGAGGTGGGG GTCTCGGAGG AACCCGAGCC CGAGCCCCCT
    GGGCCGCCGA GGAAGGGGCC GCATTTCATG GAGGGCTCAG TGACTGACCG GCGCCGCATC
    TTCGAGCGAG ACGGCAGGGC CTGCTCTACC CTCAGCCTGT CGGGGCCTGA GCTGAAGCAG
    TTCCAGCAGA GCGCACTGGC CGACTACATT CAGCGCAAGA CCGGCAAGCG GCCTGCGGGC
    GCCGGCTGTG GCCTGCAGGA GCCGGGGCGG CTGCTGGAGC GCACCCAGAG CACCTACCTC
    CAGCCCGAGG GCCCGGGCCT CGCCGCGGCT TCCAGTCTCT GCTCGCTTCG GGAGCCCAGC
    CTGCCGCCCC GCAGGGAGAC CGCCCTTCTG CCAGCCACTG TGGTGGGGAG TACTGGGGAG
    ACCCAGCGGG GCCCCCGAGA TCGCAGCAGC TCGTTTGCCA GCGGCCGCCA CCTTGGGGAA
    AGGCACCGCG GGGACTCAGC GCCGAGAGAA CTGCTCAGTG GAGCTAACAA TGGATCAAAG
    GGCCCCCAGA GACTGAACGG TACCCCAGGG GAACCCTCCC CGTGGGGGGC CACAGCTGGG
    AGGGCCGCGA AGTCCATGTC GGCTGAGGAC CTGCTGGAGC GCTCAGACGT CCAGGCTGTC
    CCTGTCCACA TGAGGTCCCG GTCATCTCCC ACCGCCGACA AGCGCCAGGA TGTGCTTTTG
    GGGGAAAACA GTAGCTTTGG TCTCATGAAG GATCCGTGTT ATTTGACTGG CCCAGGTTCC
    AGGCCATTCA GCTGTTCCAG CCGTGAAGAG ATGCCAGCGC TCAGGCACCA CCCCAGCCCT
    CACTGGGGGG ATTCGGGCTG CAAAGCAGGT AGTGGTGGTG CCTCTGTTCC CAGCGGGGGT
    CCGGGGCCGC TGGACCCTCC GAGACAGGCC AGCAGAACGC CTTGCCCTCT GCCACTGTCC
    TCGGGAGCTC ATGGGCATCT TCCAGACGCC AGGGCTGCAC CCCTGAGCTC GGTCTTGTCT
    GCCCCTGGCC CTTCGAGCTA TTGCTCACAG GCTGCAGCCC AGCCCTCCAC ACCAGCCGGC
    ACCCCCAGAA GCGGCAGTTG CCACAGCCCG GCCCAGCCTC CCAGCCCTGA GCGCAAGACA
    GAAGAGCTCG CAGGGATGCG GGCCTCCCCG CCCTGGATGA AGCGGACCTA CATTGTCAGA
    GAGGAGAGCC TCCCCGAGGA CCCCCCGAAG CACTACCCCA AGCCACCGGA TGCCCCCAGC
    TCAAGCAGCA CTTCTGACCC GGATACGCCT CTCGGGGCCC CAGGCATTCT GGGCAGGATT
    TCCCTCCGGA TCTCCGAGTC GGCCTTGCTG GCTTCCCCGC CACCTCGCGA GGACTGCGAG
    GACGACGAGG TGTTTGTGAG GGACCCACGC CCCACCGCCA CTTCCAGCCC CCGCTGCGAT
    GAGCTGCTGC TGCCCCCTCC GCCTCCGCCC CCACCACCAA CAACCAGCCA GGCATCCCTG
    GCCCAGGGCC TGGACCACTT CCCTCCGCCT CCTCCCGAAG CTATGTGCCC GGCCCAGTGG
    GAGGAGGGCT ACCTGGAGCC CCGTGCCAGC TCCAGCAAAC TTGCCAAGGT GACAGTTGCC
    AAGGAAAGGT CCATGGCCGG TGCAGCCCAT CCGGTTGATA ATCAGCTGCT GGGTTCCAGA
    TCGCAAACTT CAGGCAAGAG TTTCGAGGCC AAGGAGACCA ACCCACCCTC CACCACGGGT
    GCTCTGCCCC AGCCTGCTGG GTCTCTAAGC AAGCAGCCAA GCCCAGGACA GCCACCTCCC
    ATGCAGACCC ACAGCCTCAT CCACGAGCCA GCCATTGGGA CTGCAGGTTT AGAAAAGAAT
    GTCAGTTCTG GTCCTCAAAA GACCTCAGAA GACATCAGAA CAGAGACGCT AGCCAAGGAA
    ATTATCCACC AAGACAAATC TCTGGCAGAC ATTTTGGACC CAGACTCCAG AATGAAGACA
    ACTATGGACC TGATGGAAGG TTTGTTTCCT CGAGATGTTA ATTTGCTGAA GGAAAACAGC
    GTAAAGAAGA AGGCCGTGCA GAAAACCATC AGCTTCCCAG GATGTGAGAC CAAGAGGAGT
    GACGACAAGG AAGCAGTGGG CATGTTGGTC AACTGTCCTG CCTACTACAG CGTCTCTGCT
    CCCAAGGCTG AGCTTCTCAA CAAAATCAAA GCTATGCCAG AAGAGGTGAA CGAGGAAGAG
    GAGCAGGCAG ACATCAACGA AAAGAAGGCG GAGCTGATCG GAAGCCTCAC CCACAAGCTC
    GAGACCCTCC AGGAAGCCAA GGGGAGCCTG CTGATGGACA TCAAGCTCAA TAACGCCCTG
    GGGGAGGAGG TGGAGGCCTG GATCAGTGAG CTCTGCAAGC CCAACGAGAT CGACAAGTAC
    AAGATGTTCA TCGGGGACTT GGACAAGGTG GTGAACCTGC TCCTGTCCCT CTCGGGGCGC
    CTGGCCCGCG TGGAGAACGT GCTCAGCGGC CTTGGTGAAG ACGCCAGTAA CGAGGAAAGG
    AGTTCACTCA ACGAGAAGAG GAAGGTCCTG TCTGCTCAGC ACGAGGACGC CCGGGAGCTC
    AAGGAGAACG TGGATCGGAG GGAGCGCGTG GTGCTGGACA TCCTGGCCAA TTACCTCTCC
    GAAGAGCAGC TCCAAGATTA CCGGCATTTC GTGAAAATGA AGTCGACACT CCTCATCGAG
    CAGCGAGAGC TGGATGACAA GATCAAGCTG GGCCAGGAGC AGGTCAAGTG CCTGCTAGAG
    AGCCTTCCCT CGGACTTCGT GCCCAAGGCG GGGGCCCTGG CTCTGCCCCC AGGCCTCACC
    GGCGATGTGA CTCCCGTGGG GGGCTGGACT GGCAGTGGTG TTTTCCCAGC GGTGACCTCC
    CCACTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.