GTF3A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GTF3A
Entrez Gene ID	100135054
Full Name	general transcription factor IIIA
General protein information	Preferred Names general transcription factor IIIA Names transcription factor IIIA
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001113572.1	NP_001107044.1	transcription factor IIIA

Pathways from BioSystems

Homologies

Homo sapiens (human)	GTF3A	NP_002088.2
Mus musculus (house mouse)	Gtf3a	NP_079928.2
Rattus norvegicus (Norway rat)	Gtf3a	NP_001107042.1
Gallus gallus (chicken)	GTF3A	NP_001107043.1
Drosophila melanogaster (fruit fly)	CG9609	NP_573161.1
Magnaporthe oryzae (rice blast fungus)	MGG_05322	XP_003712886.1
Pan troglodytes (chimpanzee)	GTF3A	XP_522641.3
	GTF3A	XP_001095556.1
Canis lupus familiaris (dog)	GTF3A	XP_534523.3
Bos taurus (cattle)	GTF3A	NP_001107044.1
Danio rerio (zebrafish)	gtf3aa	NP_001003866.1
Schizosaccharomyces pombe (fission yeast)	sfc2	NP_594670.1
Xenopus tropicalis (tropical clawed frog)	gtf3a	XP_002941582.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GTF3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa40778	NM_001113572.1 Latest version!	Bos taurus general transcription factor IIIA (GTF3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GTF3A ( NM_001113572.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001107044.1 Bos taurus general transcription factor IIIA (GTF3A), mRNA.

Price & Availability↑

CloneID	OBa40778
Clone ID Related Accession (Same CDS sequence)	NM_001113572.1
Accession Version	NM_001113572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	transcription factor IIIA
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DY181718.1, CO878421.1, DN743221.1 and CN438393.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END##

CTGGAGCCGC CGGCCTCGGT GGCCGAGGCG GTGTCGTCCC TGACTATCGC CGATGCATTC 
GTTGCAGCCG GCGAGGGCGC CGCCCCGCCG CCCCAGGCGC CCCCTCGGAG CTTCATCTGT 
TCCTTCCCCA GCTGCAGCGC CGCTTACAAC AAGGCCTGGA AGCTGGACGC GCACCTGTGC 
CGGCACACAG GGGAGAGACC ATTTGTTTGT GACCATGAAG GCTGTGGCAA GGCCTTCGTC 
AGGGACTACC ATCTCAGCCG CCACGCCCTG ATTCACACCG GAGAAAAGCC CTTTGTTTGT 
ACAGCTAGTG GCTGTGAACA GAAATTCAAC ACAAAATCAA ACTTGAAGAA ACATTTTGAA 
CGCAAACATG AAAACCAGCA AAAACAATAT ACATGCAGTT TTGAAGGTTG TGAGAAGACC 
TTTAAGAAAC ATCAGCAGCT GAAAACCCAC CAGTGCCAGC ACACCAACGA GCCACTGTTC 
AAGTGTGCCC ACGAGGGATG TGGAAAACAC TTTGCCTCCC CCAGCAGTCT GAAGCGGCAT 
GGGAAGGTCC ATGAGGGCTA TATATGTCAA AAACAATGTT CTTTTGTGGC AAAAACATGG 
ACAGAGCTTC TAAAACACAT GAGAGAAACC CATAAAGAGG ACATAAGGTG TGGCGTGTGC 
CAGAAAACGT TTAAGCGCAA GGACTTCCTG CGGCAGCACA TGAGGATGCA CGCCCCCGAG 
CGGGACGTGT GTCGGTGTCC GCGGGCAGGC TGCGGCCGCA CCTACACCAC CGCGTTCAAC 
CTCCAGAGCC ACATCCTCTC CTTCCACGAG CAGCAGCGCC CCTTCGTGTG CGAGCATGCT 
GGCTGCGGCA AGACCTTTGC CATGAAGCAG AGCCTCAGCA GGCATGCCGT TGTGCATGAC 
CCCAACATGA AGAAAACCAA AGTGAGGCCG TCTCGTGGGA AGCGGAGCCT GGCCTCCCGG 
CTCAGTGGGT ACGTCCCTCC TAAGAGGACA CAGGGCCAGG CTGTGCCTTT GCCCAGGAAT 
GGGGAGCCGC TGAGCTGCAT GGAAGGCCAC GTACTCTCCA CGTCGCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107044.1
CDS	113..1186
Translation	LEPPASVAEAVSSLTIADAFVAAGEGAAPPPQAPPRSFICSFPSCSAAYNKAWKLDAHLCRHTGERPFVCDHEGCGKAFVRDYHLSRHALIHTGEKPFVCTASGCEQKFNTKSNLKKHFERKHENQQKQYTCSFEGCEKTFKKHQQLKTHQCQHTNEPLFKCAHEGCGKHFASPSSLKRHGKVHEGYICQKQCSFVAKTWTELLKHMRETHKEDIRCGVCQKTFKRKDFLRQHMRMHAPERDVCRCPRAGCGRTYTTAFNLQSHILSFHEQQRPFVCEHAGCGKTFAMKQSLSRHAVVHDPNMKKTKVRPSRGKRSLASRLSGYVPPKRTQGQAVPLPRNGEPLSCMEGHVLSTSPC

Target ORF information:

RefSeq Version	NM_001113572.1
Organism	Bos taurus(cattle)
Definition	Bos taurus general transcription factor IIIA (GTF3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113572.1

ORF Insert Sequence:

CTGGAGCCGC CGGCCTCGGT GGCCGAGGCG GTGTCGTCCC TGACTATCGC CGATGCATTC 
GTTGCAGCCG GCGAGGGCGC CGCCCCGCCG CCCCAGGCGC CCCCTCGGAG CTTCATCTGT 
TCCTTCCCCA GCTGCAGCGC CGCTTACAAC AAGGCCTGGA AGCTGGACGC GCACCTGTGC 
CGGCACACAG GGGAGAGACC ATTTGTTTGT GACCATGAAG GCTGTGGCAA GGCCTTCGTC 
AGGGACTACC ATCTCAGCCG CCACGCCCTG ATTCACACCG GAGAAAAGCC CTTTGTTTGT 
ACAGCTAGTG GCTGTGAACA GAAATTCAAC ACAAAATCAA ACTTGAAGAA ACATTTTGAA 
CGCAAACATG AAAACCAGCA AAAACAATAT ACATGCAGTT TTGAAGGTTG TGAGAAGACC 
TTTAAGAAAC ATCAGCAGCT GAAAACCCAC CAGTGCCAGC ACACCAACGA GCCACTGTTC 
AAGTGTGCCC ACGAGGGATG TGGAAAACAC TTTGCCTCCC CCAGCAGTCT GAAGCGGCAT 
GGGAAGGTCC ATGAGGGCTA TATATGTCAA AAACAATGTT CTTTTGTGGC AAAAACATGG 
ACAGAGCTTC TAAAACACAT GAGAGAAACC CATAAAGAGG ACATAAGGTG TGGCGTGTGC 
CAGAAAACGT TTAAGCGCAA GGACTTCCTG CGGCAGCACA TGAGGATGCA CGCCCCCGAG 
CGGGACGTGT GTCGGTGTCC GCGGGCAGGC TGCGGCCGCA CCTACACCAC CGCGTTCAAC 
CTCCAGAGCC ACATCCTCTC CTTCCACGAG CAGCAGCGCC CCTTCGTGTG CGAGCATGCT 
GGCTGCGGCA AGACCTTTGC CATGAAGCAG AGCCTCAGCA GGCATGCCGT TGTGCATGAC 
CCCAACATGA AGAAAACCAA AGTGAGGCCG TCTCGTGGGA AGCGGAGCCT GGCCTCCCGG 
CTCAGTGGGT ACGTCCCTCC TAAGAGGACA CAGGGCCAGG CTGTGCCTTT GCCCAGGAAT 
GGGGAGCCGC TGAGCTGCAT GGAAGGCCAC GTACTCTCCA CGTCGCCATG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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GTF3A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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