DUSP27 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DUSP27
Entrez Gene ID	100124500
Full Name	dual specificity phosphatase 27, atypical
General protein information	Preferred Names dual specificity phosphatase 27, atypical Names inactive dual specificity phosphatase 27 dual specificity phosphatase 27 (putative)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010802713.2	XP_010801015.1	inactive dual specificity phosphatase 27 isoform X2
XM_005203419.4	XP_005203476.1	inactive dual specificity phosphatase 27 isoform X1
XM_024983987.1	XP_024839755.1	inactive dual specificity phosphatase 27 isoform X2
NM_001102543.1	NP_001096013.1	inactive dual specificity phosphatase 27
XM_005203419.3	XP_005203476.1	inactive dual specificity phosphatase 27 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DUSP27	NP_001073895.1
Canis lupus familiaris (dog)	DUSP27	XP_547482.2
Gallus gallus (chicken)	DUSP27	XP_416655.3
Danio rerio (zebrafish)	LOC792623	XP_003197655.1
	DUSP27	XP_001087527.1
Rattus norvegicus (Norway rat)	Dusp27	NP_001178858.1
Xenopus tropicalis (tropical clawed frog)	dusp27	XP_004912323.1
Bos taurus (cattle)	DUSP27	NP_001096013.1
Pan troglodytes (chimpanzee)	DUSP27	XP_513970.2
Mus musculus (house mouse)	Dusp27	NP_001153521.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DUSP27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DUSP27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39103	NM_001102543.1 Latest version!	Bos taurus dual specificity phosphatase 27, atypical (DUSP27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa17091	XM_005203419.4 Latest version!	Bos taurus dual specificity phosphatase 27, atypical (DUSP27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa58679	XM_010802713.2 Latest version!	Bos taurus dual specificity phosphatase 27 (putative) (DUSP27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa58679	XM_024983987.1 Latest version!	Bos taurus dual specificity phosphatase 27, atypical (DUSP27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa17091	XM_005203419.3	Bos taurus dual specificity phosphatase 27 (putative) (DUSP27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DUSP27 ( NM_001102543.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001096013.1 Bos taurus dual specificity phosphatase 27, atypical (DUSP27), mRNA.

Price & Availability↑

CloneID	OBa39103
Clone ID Related Accession (Same CDS sequence)	NM_001102543.1
Accession Version	NM_001102543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Bos taurus(cattle)
Product	inactive dual specificity phosphatase 27
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149008.1. ##Evidence-Data-START## Transcript exon combination :: BC149008.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGAGT CTGCCGAGCA GTTGCTGGTG GAGGACCTGT ACAACCGCGT CAGGGAGAAG 
ATGGATGACA CCAGCCTGTA CAACACTCCC TGCATCCTGG ACCTCCAGCG GGCCTTGGTC 
CAAGACCGCC AAGAGGCTCC CTGGAATGAG GTGGATGAGG TCTGGCCCAA CGTCTTCATA 
GCAGAGAAGA GTGTGGCTGT GAACAAGGGG AGGCTGAAGA GGCTGGGAAT TACTCACATC 
CTGAATGCTG CTCACGGCAC TGGTGTCTAC ACGGGGCCTG AATTCTACAC TGGCCTGGAG 
ATCCAATACC TGGGTGTGGA GGTGGATGAC TTTCCTGAGG TGGATATCTC CCAGCATTTC 
CGGAAGGCAG CCGAGTTCCT GGATGAAGCC CTGCTGACCT ATAGAGGGAA AGTCCTGGTC 
AGCAGTGAAA TGGGCGTCAG CCGTTCGGCT GTGCTGGTAG TAGCCTACCT GATGATCTTC 
CACAACATGG CCGTCCTGGA GGCCTTGATG ACTGTGCGCA AGAAGCGGGC CATCTACCCC 
AACGACGGCT TCCTGAAGCA GCTCCGCGAG CTCAACGAGA AGCTGATGGA GGAGCGCGAA 
GAGGACTTCA GCAGGGAGTG TGGGGAGGAG GCAGTGGAGG AGGGCGAGGA CGCCGGGAGC 
ACGGTGGCAG CCAGAGTGCA CACCCTCACC GTGGAGGAGG AGGACGACGC TGCCAGTCAC 
CTGAGTACCT CCTCCTTGGG GAGGGCCAGC CAGGCCTCCA AGCCCCTCAC CCTTATCGAT 
GAGGAGGAGG AGGAGAGGCT CTACGAGGCG TGGAAAAAGG GGCAGAGCCT CCCCACGGGC 
AAGGTCCTGC AGGGCGAGGA CGACAGGTTC TCAGCCTCCT CTCGTCAAGG TGAGGAGCCC 
GAGGATGAGG ATGTGGAGCG AATCATCCGG GAGTGGCAAA GCCGAAATGA GAGGTACCAG 
GCAGAAGGGC ACCGGAGGTG GAATAGGGAG GAGGAAGAGG AAGAGGAGAG CAATGGCAGC 
TCCTTCGTGA GGAGAAGAAG GAGGCACACG CTGAGTGAGA GCAGCACCTC CGAGAGTGTG 
AGCAGCCATG ACATCCGGGT CCTGAAGCAG CAGCTGCAGA TGAGCAGCCA GAACCGAGGT 
GGGAGACGCC GCTCTGACTC CATGTCCACG GAGAGCACCT GGGACATGTG GGACCAGAGG 
CTGCAGGAGA TTGAGAAGGA GGCTTCCCGA AAGTACCACT CCAGGAACAA GAGAGAGGAG 
GTGGACGCCA GCTCGGAGGT GGGGAGCAGG GCGCGGGAGG ATGACGAGGA GAGTGTGGCC 
TCCGAGGCCA GCTCCTTCTA TAACTTCTGC AGCAGAAACA AGGACAAACT CACCGCCCTG 
GAAAGGTGGA AGATCAAGAG AATCCAGTTT GGATTTCACA AGAAGGACTC AGAGGCAGGA 
GATGGCAGCA GTGAGCAAGG TGCGGAGGAG GTGGAGGGGG AGAAGAAAAA CCTCTCTGAT 
GTCAATCTGT CTGCCTACCA GGCCTGGAAG CTGAAACACC AAAAGAAGGT GGGCAGTGAG 
AACAAGGAGG AGGTGGTGGA GCTCAGCAAG GGAGATGACT TAGCCTTGGT CAAGAAGCGG 
CAGCGGAGGC TGGAGCTGCT GGAGAGAAGC AGGCAGACAC TGGAGGAGAG CCAGTCAATG 
GGAAGCTGGG AGGCAGATAG CTCAGCTGCC AGTGGGAGCA TCCCCCTGTC TGCATTCTGG 
TCGGCAGCCC CCTCAGTCAG CGCTGATGGG GACACAGCAT CTGTGCTCAG CGCCCAGAGG 
AACAGCTCCC ATCCCTCTCA GGCCCTAAGC AAAGTAGGCG GATGCTTGGC TTCCACCCCC 
ACCACACCTC TACCTAACCT GCATGTGGGG CCTGGAGACA CAATCTCCAT TGCCAGCATA 
CAGAACTGGA TTGCCAACGT AGTCAGTGAA ACTCTCGCCC AGAAGCAAAA TGAAATGCTG 
TTGTCGCCCC GCCCACTGTC CGTTGTAAGC ATGAAGGCAG CACCAGCAGG CAGCTGCCTA 
GGGGATGACC AAGTCTCCAT GCTCAGTGGA CAGAGCAGCT CTGCCCTGGG TGGCTGCCTG 
CTGCCTCAAA GCCAGGCAAG ACCCAGCTCT GAGACCCAGT CTGTGTTGTC CTCCCATACC 
ATGAGTGCAA GAGCTGAAGG TACTGGGAGC AAAGTGAAGG GGACCAGCAA GCCTATCTAT 
AGCCTCTTTG CTGACAATAT TGACCTAAAG GAGCTTGGCC GGAAGGAGAA GGAGATGCAA 
ACAGAGCTGA GAGAGAAGAT GTCCGAGTAC AAAATGGAGA AGCTGGCCTC TGACAACAAA 
CGCAGCTCCC TTTTCAAGAA GAAGAAGGTC AAGGAAGATG AGGATGAGGA TGATGACGTG 
GGTGATAGGG ATGAGGACAC TGACAGCGCC ATAGGGAGCT TCCGGTATTC TTCCCGCAGT 
AATTCCCAGA AACCCGAAAC AGACACCTTC TCCTCCCTGG CTGTCTCTGA TGGCTACGGA 
AGTGGCAGCA GAGCTGGCAG AGAGAAGGAT AACAGTATTA ATAAGTGGCT CAGTGGCCTC 
AGGACAGAGG AAAAATCTCC CCAAAGTGAT TGGTCTGGAA GCTCCAGGGA AAAGTGCACC 
AGATCTTCCC TGATTCGGGA GACAGAGTCT AAGTCTTCCA GTTACAAGTT CTCCAAATCC 
CGGTCAGAGG AGCAGGACAC CTCCTCCTAC GACGAGACCA ATAGCAACTC GGTAAAAAGC 
ACTTCACGGT TCTCTGCTTC CTCCACCAGG GAAGGCAGAG AGATGCACAC ATTCTCCAGG 
TCCATGTTTA GTGAGACCTC AAGCTCCCGG GAGGAAAGCC CGGAGCCCTA CTTTTTCCGC 
CGGACCCCCG AGCCCTCTGA AGGAGAAGAG TCCCCAGAAC CACGGCGTCC AAATGGGGCC 
AGATCCAGGG ACTGGGAAGA TGTGGAAGAG TCATCCAAGT CAGACTTTTC TGAATTTGGA 
GCCAAGAGGA AATTCACCCA GAGTTTCATG AGATCTGAAG AGGAGGGAGA GAAAGCGAGG 
ATGGAAAAGA AAGAAGAAGG GAGGTTTGCT TCAGGGCGGC AGTCCCGGTA TTGGAGAAGC 
ACTGACAGGG AGGAGGAGGA GGAAATGGAT GATGAAGCCA TCATTGCTGC CTGGAGACGC 
CGGCAAGAAG AAACAAGGAC TAAGCTGCAG AGAAGAAGGG AGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096013.1
CDS	411..3638
Translation	MLESAEQLLVEDLYNRVREKMDDTSLYNTPCILDLQRALVQDRQEAPWNEVDEVWPNVFIAEKSVAVNKGRLKRLGITHILNAAHGTGVYTGPEFYTGLEIQYLGVEVDDFPEVDISQHFRKAAEFLDEALLTYRGKVLVSSEMGVSRSAVLVVAYLMIFHNMAVLEALMTVRKKRAIYPNDGFLKQLRELNEKLMEEREEDFSRECGEEAVEEGEDAGSTVAARVHTLTVEEEDDAASHLSTSSLGRASQASKPLTLIDEEEEERLYEAWKKGQSLPTGKVLQGEDDRFSASSRQGEEPEDEDVERIIREWQSRNERYQAEGHRRWNREEEEEEESNGSSFVRRRRRHTLSESSTSESVSSHDIRVLKQQLQMSSQNRGGRRRSDSMSTESTWDMWDQRLQEIEKEASRKYHSRNKREEVDASSEVGSRAREDDEESVASEASSFYNFCSRNKDKLTALERWKIKRIQFGFHKKDSEAGDGSSEQGAEEVEGEKKNLSDVNLSAYQAWKLKHQKKVGSENKEEVVELSKGDDLALVKKRQRRLELLERSRQTLEESQSMGSWEADSSAASGSIPLSAFWSAAPSVSADGDTASVLSAQRNSSHPSQALSKVGGCLASTPTTPLPNLHVGPGDTISIASIQNWIANVVSETLAQKQNEMLLSPRPLSVVSMKAAPAGSCLGDDQVSMLSGQSSSALGGCLLPQSQARPSSETQSVLSSHTMSARAEGTGSKVKGTSKPIYSLFADNIDLKELGRKEKEMQTELREKMSEYKMEKLASDNKRSSLFKKKKVKEDEDEDDDVGDRDEDTDSAIGSFRYSSRSNSQKPETDTFSSLAVSDGYGSGSRAGREKDNSINKWLSGLRTEEKSPQSDWSGSSREKCTRSSLIRETESKSSSYKFSKSRSEEQDTSSYDETNSNSVKSTSRFSASSTREGREMHTFSRSMFSETSSSREESPEPYFFRRTPEPSEGEESPEPRRPNGARSRDWEDVEESSKSDFSEFGAKRKFTQSFMRSEEEGEKARMEKKEEGRFASGRQSRYWRSTDREEEEEMDDEAIIAAWRRRQEETRTKLQRRRED

Target ORF information:

RefSeq Version	NM_001102543.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dual specificity phosphatase 27, atypical (DUSP27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102543.1

ORF Insert Sequence:

ATGCTGGAGT CTGCCGAGCA GTTGCTGGTG GAGGACCTGT ACAACCGCGT CAGGGAGAAG 
ATGGATGACA CCAGCCTGTA CAACACTCCC TGCATCCTGG ACCTCCAGCG GGCCTTGGTC 
CAAGACCGCC AAGAGGCTCC CTGGAATGAG GTGGATGAGG TCTGGCCCAA CGTCTTCATA 
GCAGAGAAGA GTGTGGCTGT GAACAAGGGG AGGCTGAAGA GGCTGGGAAT TACTCACATC 
CTGAATGCTG CTCACGGCAC TGGTGTCTAC ACGGGGCCTG AATTCTACAC TGGCCTGGAG 
ATCCAATACC TGGGTGTGGA GGTGGATGAC TTTCCTGAGG TGGATATCTC CCAGCATTTC 
CGGAAGGCAG CCGAGTTCCT GGATGAAGCC CTGCTGACCT ATAGAGGGAA AGTCCTGGTC 
AGCAGTGAAA TGGGCGTCAG CCGTTCGGCT GTGCTGGTAG TAGCCTACCT GATGATCTTC 
CACAACATGG CCGTCCTGGA GGCCTTGATG ACTGTGCGCA AGAAGCGGGC CATCTACCCC 
AACGACGGCT TCCTGAAGCA GCTCCGCGAG CTCAACGAGA AGCTGATGGA GGAGCGCGAA 
GAGGACTTCA GCAGGGAGTG TGGGGAGGAG GCAGTGGAGG AGGGCGAGGA CGCCGGGAGC 
ACGGTGGCAG CCAGAGTGCA CACCCTCACC GTGGAGGAGG AGGACGACGC TGCCAGTCAC 
CTGAGTACCT CCTCCTTGGG GAGGGCCAGC CAGGCCTCCA AGCCCCTCAC CCTTATCGAT 
GAGGAGGAGG AGGAGAGGCT CTACGAGGCG TGGAAAAAGG GGCAGAGCCT CCCCACGGGC 
AAGGTCCTGC AGGGCGAGGA CGACAGGTTC TCAGCCTCCT CTCGTCAAGG TGAGGAGCCC 
GAGGATGAGG ATGTGGAGCG AATCATCCGG GAGTGGCAAA GCCGAAATGA GAGGTACCAG 
GCAGAAGGGC ACCGGAGGTG GAATAGGGAG GAGGAAGAGG AAGAGGAGAG CAATGGCAGC 
TCCTTCGTGA GGAGAAGAAG GAGGCACACG CTGAGTGAGA GCAGCACCTC CGAGAGTGTG 
AGCAGCCATG ACATCCGGGT CCTGAAGCAG CAGCTGCAGA TGAGCAGCCA GAACCGAGGT 
GGGAGACGCC GCTCTGACTC CATGTCCACG GAGAGCACCT GGGACATGTG GGACCAGAGG 
CTGCAGGAGA TTGAGAAGGA GGCTTCCCGA AAGTACCACT CCAGGAACAA GAGAGAGGAG 
GTGGACGCCA GCTCGGAGGT GGGGAGCAGG GCGCGGGAGG ATGACGAGGA GAGTGTGGCC 
TCCGAGGCCA GCTCCTTCTA TAACTTCTGC AGCAGAAACA AGGACAAACT CACCGCCCTG 
GAAAGGTGGA AGATCAAGAG AATCCAGTTT GGATTTCACA AGAAGGACTC AGAGGCAGGA 
GATGGCAGCA GTGAGCAAGG TGCGGAGGAG GTGGAGGGGG AGAAGAAAAA CCTCTCTGAT 
GTCAATCTGT CTGCCTACCA GGCCTGGAAG CTGAAACACC AAAAGAAGGT GGGCAGTGAG 
AACAAGGAGG AGGTGGTGGA GCTCAGCAAG GGAGATGACT TAGCCTTGGT CAAGAAGCGG 
CAGCGGAGGC TGGAGCTGCT GGAGAGAAGC AGGCAGACAC TGGAGGAGAG CCAGTCAATG 
GGAAGCTGGG AGGCAGATAG CTCAGCTGCC AGTGGGAGCA TCCCCCTGTC TGCATTCTGG 
TCGGCAGCCC CCTCAGTCAG CGCTGATGGG GACACAGCAT CTGTGCTCAG CGCCCAGAGG 
AACAGCTCCC ATCCCTCTCA GGCCCTAAGC AAAGTAGGCG GATGCTTGGC TTCCACCCCC 
ACCACACCTC TACCTAACCT GCATGTGGGG CCTGGAGACA CAATCTCCAT TGCCAGCATA 
CAGAACTGGA TTGCCAACGT AGTCAGTGAA ACTCTCGCCC AGAAGCAAAA TGAAATGCTG 
TTGTCGCCCC GCCCACTGTC CGTTGTAAGC ATGAAGGCAG CACCAGCAGG CAGCTGCCTA 
GGGGATGACC AAGTCTCCAT GCTCAGTGGA CAGAGCAGCT CTGCCCTGGG TGGCTGCCTG 
CTGCCTCAAA GCCAGGCAAG ACCCAGCTCT GAGACCCAGT CTGTGTTGTC CTCCCATACC 
ATGAGTGCAA GAGCTGAAGG TACTGGGAGC AAAGTGAAGG GGACCAGCAA GCCTATCTAT 
AGCCTCTTTG CTGACAATAT TGACCTAAAG GAGCTTGGCC GGAAGGAGAA GGAGATGCAA 
ACAGAGCTGA GAGAGAAGAT GTCCGAGTAC AAAATGGAGA AGCTGGCCTC TGACAACAAA 
CGCAGCTCCC TTTTCAAGAA GAAGAAGGTC AAGGAAGATG AGGATGAGGA TGATGACGTG 
GGTGATAGGG ATGAGGACAC TGACAGCGCC ATAGGGAGCT TCCGGTATTC TTCCCGCAGT 
AATTCCCAGA AACCCGAAAC AGACACCTTC TCCTCCCTGG CTGTCTCTGA TGGCTACGGA 
AGTGGCAGCA GAGCTGGCAG AGAGAAGGAT AACAGTATTA ATAAGTGGCT CAGTGGCCTC 
AGGACAGAGG AAAAATCTCC CCAAAGTGAT TGGTCTGGAA GCTCCAGGGA AAAGTGCACC 
AGATCTTCCC TGATTCGGGA GACAGAGTCT AAGTCTTCCA GTTACAAGTT CTCCAAATCC 
CGGTCAGAGG AGCAGGACAC CTCCTCCTAC GACGAGACCA ATAGCAACTC GGTAAAAAGC 
ACTTCACGGT TCTCTGCTTC CTCCACCAGG GAAGGCAGAG AGATGCACAC ATTCTCCAGG 
TCCATGTTTA GTGAGACCTC AAGCTCCCGG GAGGAAAGCC CGGAGCCCTA CTTTTTCCGC 
CGGACCCCCG AGCCCTCTGA AGGAGAAGAG TCCCCAGAAC CACGGCGTCC AAATGGGGCC 
AGATCCAGGG ACTGGGAAGA TGTGGAAGAG TCATCCAAGT CAGACTTTTC TGAATTTGGA 
GCCAAGAGGA AATTCACCCA GAGTTTCATG AGATCTGAAG AGGAGGGAGA GAAAGCGAGG 
ATGGAAAAGA AAGAAGAAGG GAGGTTTGCT TCAGGGCGGC AGTCCCGGTA TTGGAGAAGC 
ACTGACAGGG AGGAGGAGGA GGAAATGGAT GATGAAGCCA TCATTGCTGC CTGGAGACGC 
CGGCAAGAAG AAACAAGGAC TAAGCTGCAG AGAAGAAGGG AGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUSP27 ( XM_005203419.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203476.1 Bos taurus dual specificity phosphatase 27, atypical (DUSP27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa17091
Clone ID Related Accession (Same CDS sequence)	XM_005203419.4 , XM_005203419.3
Accession Version	XM_005203419.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive dual specificity phosphatase 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203419.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACCA GTGGGGACCC AGAAGAGGAG CAGGTGGTCC CGAGTGAAGA GGATGAAGCC 
AACGTGAGGG CTGTACAGGC CCGGTACCTC CGGAGCCCCT CCCCCAGCCA GTACTCAATG 
GTCTCAGATG CAGAAACAGA AAGCATTTTC ATGGATCCCA TTCACCTCTC CTCAGCTGTT 
GCAGCTAAAC AGATCATCAA TGAAGAACTC AAGCCACGTG GGGTCAGAGT CGAGGCAGAG 
TGTCCAGGCA TGCTGGAGTC TGCCGAGCAG TTGCTGGTGG AGGACCTGTA CAACCGCGTC 
AGGGAGAAGA TGGATGACAC CAGCCTGTAC AACACTCCCT GCATCCTGGA CCTCCAGCGG 
GCCTTGGTCC AAGACCGCCA AGAGGCTCCC TGGAATGAGG TGGATGAGGT CTGGCCCAAC 
GTCTTCATAG CAGAGAAGAG TGTGGCTGTG AACAAGGGGA GGCTGAAGAG GCTGGGAATT 
ACTCACATCC TGAATGCTGC TCACGGCACT GGTGTCTACA CGGGGCCTGA ATTCTACACT 
GGCCTGGAGA TCCAATACCT GGGTGTGGAG GTGGATGACT TTCCTGAGGT GGATATCTCC 
CAGCATTTCC GGAAGGCAGC CGAGTTCCTG GATGAAGCCC TGCTGACCTA TAGAGGGAAA 
GTCCTGGTCA GCAGTGAAAT GGGCGTCAGC CGTTCGGCTG TGCTGGTAGT AGCCTACCTG 
ATGATCTTCC ACAACATGGC CGTCCTGGAG GCCTTGATGA CTGTGCGCAA GAAGCGGGCC 
ATCTACCCCA ACGACGGCTT CCTGAAGCAG CTCCGCGAGC TCAACGAGAA GCTGATGGAG 
GAGCGCGAAG AGGACTTCAG CAGGGAGTGT GGGGAGGAGG CAGTGGAGGA GGGCGAGGAC 
GCCGGGAGCA CGGTGGCAGC CAGAGTGCAC ACCCTCACCG TGGAGGAGGA GGACGACGCT 
GCCAGTCACC TGAGTACCTC CTCCTTGGGG AGGGCCAGCC AGGCCTCCAA GCCCCTCACC 
CTTATCGATG AGGAGGAGGA GGAGAGGCTC TACGAGGCGT GGAAAAAGGG GCAGAGCCTC 
CCCACGGGCA AGGTCCTGCA GGGCGAGGAC GACAGGTTCT CAGCCTCCTC TCGTCAAGGT 
GAGGAGCCCG AGGATGAGGA TGTGGAGCGA ATCATCCGGG AGTGGCAAAG CCGAAATGAG 
AGGTACCAGG CAGAAGGGCA CCGGAGGTGG AATAGGGAGG AGGAAGAGGA AGAGGAGAGC 
AATGGCAGCT CCTTCGTGAG GAGAAGAAGG AGGCACACGC TGAGTGAGAG CAGCACCTCC 
GAGAGTGTGA GCAGCCATGA CATCCGGGTC CTGAAGCAGC AGCTGCAGAT GAGCAGCCAG 
AACCGAGGTG GGAGACGCCG CTCTGACTCC ATGTCCACGG AGAGCACCTG GGACATGTGG 
GACCAGAGGC TGCAGGAGAT TGAGAAGGAG GCTTCCCGAA AGTACCACTC CAGGAACAAG 
AGAGAGGAGG TGGACGCCAG CTCGGAGGTG GGGAGCAGGG CGCGGGAGGA TGACGAGGAG 
AGTGTGGCCT CCGAGGCCAG CTCCTTCTAT AACTTCTGCA GCAGAAACAA GGACAAACTC 
ACCGCCCTGG AAAGGTGGAA GATCAAGAGA ATCCAGTTTG GATTTCACAA GAAGGACTCA 
GAGGCAGGAG ATGGCAGCAG TGAGCAAGGT GCGGAGGAGG TGGAGGGGGA GAAGAAAAAC 
CTCTCTGATG TCAATCTGTC TGCCTACCAG GCCTGGAAGC TGAAACACCA AAAGAAGGTG 
GGCAGTGAGA ACAAGGAGGA GGTGGTGGAG CTCAGCAAGG GAGATGACTT AGCCTTGGTC 
AAGAAGCGGC AGCGGAGGCT GGAGCTGCTG GAGAGAAGCA GGCAGACACT GGAGGAGAGC 
CAGTCAATGG GAAGCTGGGA GGCAGATAGC TCAGCTGCCA GTGGGAGCAT CCCCCTGTCT 
GCATTCTGGT CGGCAGCCCC CTCAGTCAGC GCTGATGGGG ACACAGCATC TGTGCTCAGC 
GCCCAGAGCC ACAGCTCCCA TCCCTCTCAG GCCCTAAGCA AAGTAGGCGG ATGCCTGGCT 
TCCACCCCCA CCACACCTCT ACCTAACCTG CATGTGGGGC CTGGAGACAC AATCTCCATT 
GCCAGCATAC AGAACTGGAT TGCCAACGTA GTCAGTGAAA CTCTCGCCCA GAAGCAAAAT 
GAAATGCTGT TGTCGCCCCG CCCACTGTCC GTTGTAAGCA TGAAGGCAGC ACCAGCAGGC 
AGCTGCCTAG GGGATGACCA AGTCTCCATG CTCAGTGGAC AGAGCAGCTC TGCCCTGGGT 
GGCTGCCTGC TGCCTCAAAG CCAGGCAAGA CCCAGCTCTG AGACCCAGTC TGTGTTGTCC 
TCCCATACCA TGAGTGCAAG AGCTGAAGGT ACTGGGAGCA AAGTGAAGGG GACCAGCAAG 
CCTATCTATA GCCTCTTTGC TGACAATATT GACCTAAAGG AGCTTGGCCG GAAGGAGAAG 
GAGATGCAAA CAGAGCTGAG AGAGAAGATG TCCGAGTACA AAATGGAGAA GCTGGCCTCC 
GACAACAAAC GCAGCTCCCT TTTCAAGAAG AAGAAGGTCA AGGAAGATGA GGATGAGGAT 
GATGACGTGG GTGATAGGGA TGAGGACACT GACAGCGCCA TAGGGAGCTT CCGGTATTCT 
TCCCGCAGTA ATTCCCAGAA ACCCGAAACA GACACCTTCT CCTCCCTGGC TGTCTCTGAT 
GGCTACGGAA GTGGCAGCAG AGCTGGCAGA GAGAAGGATA ACAGTATTAA TAAGTGGCTC 
AGTGGCCTCA GGACAGAGGA AAAATCTCCC CAAAGTGATT GGTCTGGAAG CTCCAGGGAA 
AAGTGCACCA GATCTTCCCT GATTCGGGAG ACAGAGTCTA AGTCTTCCAG TTACAAGTTC 
TCCAAATCCC GGTCAGAGGA GCAGGACACC TCCTCCTACG ACGAGACCAA TAGCAACTCG 
GTAAAAAGCA CTTCACGGTT CTCTGCTTCC TCCACCAGGG AAGGCAGAGA GATGCACACA 
TTCTCCAGGT CCATGTTTAG TGAGACCTCA AGCTCCCGGG AGGAAAGCCC GGAGCCCTAC 
TTTTTCCGCC GGACCCCCGA GCCCTCTGAA GGAGAAGAGT CCCCAGAACC ACGGCGTCCA 
AATGGGACCA GATCCAGGGA CTGGGAAGAT GTGGAAGAGT CATCCAAGTC AGACTTTTCT 
GAATTTGGAG CCAAGAGGAA ATTCACCCAG AGTTTCATGA GATCTGAAGA GGAGGGAGAG 
AAAGCGAGGA TGGAAAAGAA AGAAGAAGGG AGGTTTGCTT CAGGGCGGCA GTCCCGGTAT 
TGGAGAAGCA CTGACAGGGA GGAGGAGGAG GAAATGGATG ATGAAGCCAT CATTGCTGCC 
TGGAGACGCC GGCAAGAAGA AACAAGGACT AAGCTGCAGA GAAGAAGGGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203476.1
CDS	147..3623
Translation	MATSGDPEEEQVVPSEEDEANVRAVQARYLRSPSPSQYSMVSDAETESIFMDPIHLSSAVAAKQIINEELKPRGVRVEAECPGMLESAEQLLVEDLYNRVREKMDDTSLYNTPCILDLQRALVQDRQEAPWNEVDEVWPNVFIAEKSVAVNKGRLKRLGITHILNAAHGTGVYTGPEFYTGLEIQYLGVEVDDFPEVDISQHFRKAAEFLDEALLTYRGKVLVSSEMGVSRSAVLVVAYLMIFHNMAVLEALMTVRKKRAIYPNDGFLKQLRELNEKLMEEREEDFSRECGEEAVEEGEDAGSTVAARVHTLTVEEEDDAASHLSTSSLGRASQASKPLTLIDEEEEERLYEAWKKGQSLPTGKVLQGEDDRFSASSRQGEEPEDEDVERIIREWQSRNERYQAEGHRRWNREEEEEEESNGSSFVRRRRRHTLSESSTSESVSSHDIRVLKQQLQMSSQNRGGRRRSDSMSTESTWDMWDQRLQEIEKEASRKYHSRNKREEVDASSEVGSRAREDDEESVASEASSFYNFCSRNKDKLTALERWKIKRIQFGFHKKDSEAGDGSSEQGAEEVEGEKKNLSDVNLSAYQAWKLKHQKKVGSENKEEVVELSKGDDLALVKKRQRRLELLERSRQTLEESQSMGSWEADSSAASGSIPLSAFWSAAPSVSADGDTASVLSAQSHSSHPSQALSKVGGCLASTPTTPLPNLHVGPGDTISIASIQNWIANVVSETLAQKQNEMLLSPRPLSVVSMKAAPAGSCLGDDQVSMLSGQSSSALGGCLLPQSQARPSSETQSVLSSHTMSARAEGTGSKVKGTSKPIYSLFADNIDLKELGRKEKEMQTELREKMSEYKMEKLASDNKRSSLFKKKKVKEDEDEDDDVGDRDEDTDSAIGSFRYSSRSNSQKPETDTFSSLAVSDGYGSGSRAGREKDNSINKWLSGLRTEEKSPQSDWSGSSREKCTRSSLIRETESKSSSYKFSKSRSEEQDTSSYDETNSNSVKSTSRFSASSTREGREMHTFSRSMFSETSSSREESPEPYFFRRTPEPSEGEESPEPRRPNGTRSRDWEDVEESSKSDFSEFGAKRKFTQSFMRSEEEGEKARMEKKEEGRFASGRQSRYWRSTDREEEEEMDDEAIIAAWRRRQEETRTKLQRRRED

Target ORF information:

RefSeq Version	XM_005203419.4
Organism	Bos taurus(cattle)
Definition	Bos taurus dual specificity phosphatase 27, atypical (DUSP27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203419.4

ORF Insert Sequence:

ATGGCAACCA GTGGGGACCC AGAAGAGGAG CAGGTGGTCC CGAGTGAAGA GGATGAAGCC 
AACGTGAGGG CTGTACAGGC CCGGTACCTC CGGAGCCCCT CCCCCAGCCA GTACTCAATG 
GTCTCAGATG CAGAAACAGA AAGCATTTTC ATGGATCCCA TTCACCTCTC CTCAGCTGTT 
GCAGCTAAAC AGATCATCAA TGAAGAACTC AAGCCACGTG GGGTCAGAGT CGAGGCAGAG 
TGTCCAGGCA TGCTGGAGTC TGCCGAGCAG TTGCTGGTGG AGGACCTGTA CAACCGCGTC 
AGGGAGAAGA TGGATGACAC CAGCCTGTAC AACACTCCCT GCATCCTGGA CCTCCAGCGG 
GCCTTGGTCC AAGACCGCCA AGAGGCTCCC TGGAATGAGG TGGATGAGGT CTGGCCCAAC 
GTCTTCATAG CAGAGAAGAG TGTGGCTGTG AACAAGGGGA GGCTGAAGAG GCTGGGAATT 
ACTCACATCC TGAATGCTGC TCACGGCACT GGTGTCTACA CGGGGCCTGA ATTCTACACT 
GGCCTGGAGA TCCAATACCT GGGTGTGGAG GTGGATGACT TTCCTGAGGT GGATATCTCC 
CAGCATTTCC GGAAGGCAGC CGAGTTCCTG GATGAAGCCC TGCTGACCTA TAGAGGGAAA 
GTCCTGGTCA GCAGTGAAAT GGGCGTCAGC CGTTCGGCTG TGCTGGTAGT AGCCTACCTG 
ATGATCTTCC ACAACATGGC CGTCCTGGAG GCCTTGATGA CTGTGCGCAA GAAGCGGGCC 
ATCTACCCCA ACGACGGCTT CCTGAAGCAG CTCCGCGAGC TCAACGAGAA GCTGATGGAG 
GAGCGCGAAG AGGACTTCAG CAGGGAGTGT GGGGAGGAGG CAGTGGAGGA GGGCGAGGAC 
GCCGGGAGCA CGGTGGCAGC CAGAGTGCAC ACCCTCACCG TGGAGGAGGA GGACGACGCT 
GCCAGTCACC TGAGTACCTC CTCCTTGGGG AGGGCCAGCC AGGCCTCCAA GCCCCTCACC 
CTTATCGATG AGGAGGAGGA GGAGAGGCTC TACGAGGCGT GGAAAAAGGG GCAGAGCCTC 
CCCACGGGCA AGGTCCTGCA GGGCGAGGAC GACAGGTTCT CAGCCTCCTC TCGTCAAGGT 
GAGGAGCCCG AGGATGAGGA TGTGGAGCGA ATCATCCGGG AGTGGCAAAG CCGAAATGAG 
AGGTACCAGG CAGAAGGGCA CCGGAGGTGG AATAGGGAGG AGGAAGAGGA AGAGGAGAGC 
AATGGCAGCT CCTTCGTGAG GAGAAGAAGG AGGCACACGC TGAGTGAGAG CAGCACCTCC 
GAGAGTGTGA GCAGCCATGA CATCCGGGTC CTGAAGCAGC AGCTGCAGAT GAGCAGCCAG 
AACCGAGGTG GGAGACGCCG CTCTGACTCC ATGTCCACGG AGAGCACCTG GGACATGTGG 
GACCAGAGGC TGCAGGAGAT TGAGAAGGAG GCTTCCCGAA AGTACCACTC CAGGAACAAG 
AGAGAGGAGG TGGACGCCAG CTCGGAGGTG GGGAGCAGGG CGCGGGAGGA TGACGAGGAG 
AGTGTGGCCT CCGAGGCCAG CTCCTTCTAT AACTTCTGCA GCAGAAACAA GGACAAACTC 
ACCGCCCTGG AAAGGTGGAA GATCAAGAGA ATCCAGTTTG GATTTCACAA GAAGGACTCA 
GAGGCAGGAG ATGGCAGCAG TGAGCAAGGT GCGGAGGAGG TGGAGGGGGA GAAGAAAAAC 
CTCTCTGATG TCAATCTGTC TGCCTACCAG GCCTGGAAGC TGAAACACCA AAAGAAGGTG 
GGCAGTGAGA ACAAGGAGGA GGTGGTGGAG CTCAGCAAGG GAGATGACTT AGCCTTGGTC 
AAGAAGCGGC AGCGGAGGCT GGAGCTGCTG GAGAGAAGCA GGCAGACACT GGAGGAGAGC 
CAGTCAATGG GAAGCTGGGA GGCAGATAGC TCAGCTGCCA GTGGGAGCAT CCCCCTGTCT 
GCATTCTGGT CGGCAGCCCC CTCAGTCAGC GCTGATGGGG ACACAGCATC TGTGCTCAGC 
GCCCAGAGCC ACAGCTCCCA TCCCTCTCAG GCCCTAAGCA AAGTAGGCGG ATGCCTGGCT 
TCCACCCCCA CCACACCTCT ACCTAACCTG CATGTGGGGC CTGGAGACAC AATCTCCATT 
GCCAGCATAC AGAACTGGAT TGCCAACGTA GTCAGTGAAA CTCTCGCCCA GAAGCAAAAT 
GAAATGCTGT TGTCGCCCCG CCCACTGTCC GTTGTAAGCA TGAAGGCAGC ACCAGCAGGC 
AGCTGCCTAG GGGATGACCA AGTCTCCATG CTCAGTGGAC AGAGCAGCTC TGCCCTGGGT 
GGCTGCCTGC TGCCTCAAAG CCAGGCAAGA CCCAGCTCTG AGACCCAGTC TGTGTTGTCC 
TCCCATACCA TGAGTGCAAG AGCTGAAGGT ACTGGGAGCA AAGTGAAGGG GACCAGCAAG 
CCTATCTATA GCCTCTTTGC TGACAATATT GACCTAAAGG AGCTTGGCCG GAAGGAGAAG 
GAGATGCAAA CAGAGCTGAG AGAGAAGATG TCCGAGTACA AAATGGAGAA GCTGGCCTCC 
GACAACAAAC GCAGCTCCCT TTTCAAGAAG AAGAAGGTCA AGGAAGATGA GGATGAGGAT 
GATGACGTGG GTGATAGGGA TGAGGACACT GACAGCGCCA TAGGGAGCTT CCGGTATTCT 
TCCCGCAGTA ATTCCCAGAA ACCCGAAACA GACACCTTCT CCTCCCTGGC TGTCTCTGAT 
GGCTACGGAA GTGGCAGCAG AGCTGGCAGA GAGAAGGATA ACAGTATTAA TAAGTGGCTC 
AGTGGCCTCA GGACAGAGGA AAAATCTCCC CAAAGTGATT GGTCTGGAAG CTCCAGGGAA 
AAGTGCACCA GATCTTCCCT GATTCGGGAG ACAGAGTCTA AGTCTTCCAG TTACAAGTTC 
TCCAAATCCC GGTCAGAGGA GCAGGACACC TCCTCCTACG ACGAGACCAA TAGCAACTCG 
GTAAAAAGCA CTTCACGGTT CTCTGCTTCC TCCACCAGGG AAGGCAGAGA GATGCACACA 
TTCTCCAGGT CCATGTTTAG TGAGACCTCA AGCTCCCGGG AGGAAAGCCC GGAGCCCTAC 
TTTTTCCGCC GGACCCCCGA GCCCTCTGAA GGAGAAGAGT CCCCAGAACC ACGGCGTCCA 
AATGGGACCA GATCCAGGGA CTGGGAAGAT GTGGAAGAGT CATCCAAGTC AGACTTTTCT 
GAATTTGGAG CCAAGAGGAA ATTCACCCAG AGTTTCATGA GATCTGAAGA GGAGGGAGAG 
AAAGCGAGGA TGGAAAAGAA AGAAGAAGGG AGGTTTGCTT CAGGGCGGCA GTCCCGGTAT 
TGGAGAAGCA CTGACAGGGA GGAGGAGGAG GAAATGGATG ATGAAGCCAT CATTGCTGCC 
TGGAGACGCC GGCAAGAAGA AACAAGGACT AAGCTGCAGA GAAGAAGGGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUSP27 ( XM_005203419.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203476.1 Bos taurus dual specificity phosphatase 27 (putative) (DUSP27), transcript variant X1, mRNA.

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CloneID	OBa17091
Clone ID Related Accession (Same CDS sequence)	XM_005203419.4 , XM_005203419.3
Accession Version	XM_005203419.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive dual specificity phosphatase 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103855.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203419.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACCA GTGGGGACCC AGAAGAGGAG CAGGTGGTCC CGAGTGAAGA GGATGAAGCC 
AACGTGAGGG CTGTACAGGC CCGGTACCTC CGGAGCCCCT CCCCCAGCCA GTACTCAATG 
GTCTCAGATG CAGAAACAGA AAGCATTTTC ATGGATCCCA TTCACCTCTC CTCAGCTGTT 
GCAGCTAAAC AGATCATCAA TGAAGAACTC AAGCCACGTG GGGTCAGAGT CGAGGCAGAG 
TGTCCAGGCA TGCTGGAGTC TGCCGAGCAG TTGCTGGTGG AGGACCTGTA CAACCGCGTC 
AGGGAGAAGA TGGATGACAC CAGCCTGTAC AACACTCCCT GCATCCTGGA CCTCCAGCGG 
GCCTTGGTCC AAGACCGCCA AGAGGCTCCC TGGAATGAGG TGGATGAGGT CTGGCCCAAC 
GTCTTCATAG CAGAGAAGAG TGTGGCTGTG AACAAGGGGA GGCTGAAGAG GCTGGGAATT 
ACTCACATCC TGAATGCTGC TCACGGCACT GGTGTCTACA CGGGGCCTGA ATTCTACACT 
GGCCTGGAGA TCCAATACCT GGGTGTGGAG GTGGATGACT TTCCTGAGGT GGATATCTCC 
CAGCATTTCC GGAAGGCAGC CGAGTTCCTG GATGAAGCCC TGCTGACCTA TAGAGGGAAA 
GTCCTGGTCA GCAGTGAAAT GGGCGTCAGC CGTTCGGCTG TGCTGGTAGT AGCCTACCTG 
ATGATCTTCC ACAACATGGC CGTCCTGGAG GCCTTGATGA CTGTGCGCAA GAAGCGGGCC 
ATCTACCCCA ACGACGGCTT CCTGAAGCAG CTCCGCGAGC TCAACGAGAA GCTGATGGAG 
GAGCGCGAAG AGGACTTCAG CAGGGAGTGT GGGGAGGAGG CAGTGGAGGA GGGCGAGGAC 
GCCGGGAGCA CGGTGGCAGC CAGAGTGCAC ACCCTCACCG TGGAGGAGGA GGACGACGCT 
GCCAGTCACC TGAGTACCTC CTCCTTGGGG AGGGCCAGCC AGGCCTCCAA GCCCCTCACC 
CTTATCGATG AGGAGGAGGA GGAGAGGCTC TACGAGGCGT GGAAAAAGGG GCAGAGCCTC 
CCCACGGGCA AGGTCCTGCA GGGCGAGGAC GACAGGTTCT CAGCCTCCTC TCGTCAAGGT 
GAGGAGCCCG AGGATGAGGA TGTGGAGCGA ATCATCCGGG AGTGGCAAAG CCGAAATGAG 
AGGTACCAGG CAGAAGGGCA CCGGAGGTGG AATAGGGAGG AGGAAGAGGA AGAGGAGAGC 
AATGGCAGCT CCTTCGTGAG GAGAAGAAGG AGGCACACGC TGAGTGAGAG CAGCACCTCC 
GAGAGTGTGA GCAGCCATGA CATCCGGGTC CTGAAGCAGC AGCTGCAGAT GAGCAGCCAG 
AACCGAGGTG GGAGACGCCG CTCTGACTCC ATGTCCACGG AGAGCACCTG GGACATGTGG 
GACCAGAGGC TGCAGGAGAT TGAGAAGGAG GCTTCCCGAA AGTACCACTC CAGGAACAAG 
AGAGAGGAGG TGGACGCCAG CTCGGAGGTG GGGAGCAGGG CGCGGGAGGA TGACGAGGAG 
AGTGTGGCCT CCGAGGCCAG CTCCTTCTAT AACTTCTGCA GCAGAAACAA GGACAAACTC 
ACCGCCCTGG AAAGGTGGAA GATCAAGAGA ATCCAGTTTG GATTTCACAA GAAGGACTCA 
GAGGCAGGAG ATGGCAGCAG TGAGCAAGGT GCGGAGGAGG TGGAGGGGGA GAAGAAAAAC 
CTCTCTGATG TCAATCTGTC TGCCTACCAG GCCTGGAAGC TGAAACACCA AAAGAAGGTG 
GGCAGTGAGA ACAAGGAGGA GGTGGTGGAG CTCAGCAAGG GAGATGACTT AGCCTTGGTC 
AAGAAGCGGC AGCGGAGGCT GGAGCTGCTG GAGAGAAGCA GGCAGACACT GGAGGAGAGC 
CAGTCAATGG GAAGCTGGGA GGCAGATAGC TCAGCTGCCA GTGGGAGCAT CCCCCTGTCT 
GCATTCTGGT CGGCAGCCCC CTCAGTCAGC GCTGATGGGG ACACAGCATC TGTGCTCAGC 
GCCCAGAGCC ACAGCTCCCA TCCCTCTCAG GCCCTAAGCA AAGTAGGCGG ATGCCTGGCT 
TCCACCCCCA CCACACCTCT ACCTAACCTG CATGTGGGGC CTGGAGACAC AATCTCCATT 
GCCAGCATAC AGAACTGGAT TGCCAACGTA GTCAGTGAAA CTCTCGCCCA GAAGCAAAAT 
GAAATGCTGT TGTCGCCCCG CCCACTGTCC GTTGTAAGCA TGAAGGCAGC ACCAGCAGGC 
AGCTGCCTAG GGGATGACCA AGTCTCCATG CTCAGTGGAC AGAGCAGCTC TGCCCTGGGT 
GGCTGCCTGC TGCCTCAAAG CCAGGCAAGA CCCAGCTCTG AGACCCAGTC TGTGTTGTCC 
TCCCATACCA TGAGTGCAAG AGCTGAAGGT ACTGGGAGCA AAGTGAAGGG GACCAGCAAG 
CCTATCTATA GCCTCTTTGC TGACAATATT GACCTAAAGG AGCTTGGCCG GAAGGAGAAG 
GAGATGCAAA CAGAGCTGAG AGAGAAGATG TCCGAGTACA AAATGGAGAA GCTGGCCTCC 
GACAACAAAC GCAGCTCCCT TTTCAAGAAG AAGAAGGTCA AGGAAGATGA GGATGAGGAT 
GATGACGTGG GTGATAGGGA TGAGGACACT GACAGCGCCA TAGGGAGCTT CCGGTATTCT 
TCCCGCAGTA ATTCCCAGAA ACCCGAAACA GACACCTTCT CCTCCCTGGC TGTCTCTGAT 
GGCTACGGAA GTGGCAGCAG AGCTGGCAGA GAGAAGGATA ACAGTATTAA TAAGTGGCTC 
AGTGGCCTCA GGACAGAGGA AAAATCTCCC CAAAGTGATT GGTCTGGAAG CTCCAGGGAA 
AAGTGCACCA GATCTTCCCT GATTCGGGAG ACAGAGTCTA AGTCTTCCAG TTACAAGTTC 
TCCAAATCCC GGTCAGAGGA GCAGGACACC TCCTCCTACG ACGAGACCAA TAGCAACTCG 
GTAAAAAGCA CTTCACGGTT CTCTGCTTCC TCCACCAGGG AAGGCAGAGA GATGCACACA 
TTCTCCAGGT CCATGTTTAG TGAGACCTCA AGCTCCCGGG AGGAAAGCCC GGAGCCCTAC 
TTTTTCCGCC GGACCCCCGA GCCCTCTGAA GGAGAAGAGT CCCCAGAACC ACGGCGTCCA 
AATGGGACCA GATCCAGGGA CTGGGAAGAT GTGGAAGAGT CATCCAAGTC AGACTTTTCT 
GAATTTGGAG CCAAGAGGAA ATTCACCCAG AGTTTCATGA GATCTGAAGA GGAGGGAGAG 
AAAGCGAGGA TGGAAAAGAA AGAAGAAGGG AGGTTTGCTT CAGGGCGGCA GTCCCGGTAT 
TGGAGAAGCA CTGACAGGGA GGAGGAGGAG GAAATGGATG ATGAAGCCAT CATTGCTGCC 
TGGAGACGCC GGCAAGAAGA AACAAGGACT AAGCTGCAGA GAAGAAGGGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203476.1
CDS	150..3626
Translation	MATSGDPEEEQVVPSEEDEANVRAVQARYLRSPSPSQYSMVSDAETESIFMDPIHLSSAVAAKQIINEELKPRGVRVEAECPGMLESAEQLLVEDLYNRVREKMDDTSLYNTPCILDLQRALVQDRQEAPWNEVDEVWPNVFIAEKSVAVNKGRLKRLGITHILNAAHGTGVYTGPEFYTGLEIQYLGVEVDDFPEVDISQHFRKAAEFLDEALLTYRGKVLVSSEMGVSRSAVLVVAYLMIFHNMAVLEALMTVRKKRAIYPNDGFLKQLRELNEKLMEEREEDFSRECGEEAVEEGEDAGSTVAARVHTLTVEEEDDAASHLSTSSLGRASQASKPLTLIDEEEEERLYEAWKKGQSLPTGKVLQGEDDRFSASSRQGEEPEDEDVERIIREWQSRNERYQAEGHRRWNREEEEEEESNGSSFVRRRRRHTLSESSTSESVSSHDIRVLKQQLQMSSQNRGGRRRSDSMSTESTWDMWDQRLQEIEKEASRKYHSRNKREEVDASSEVGSRAREDDEESVASEASSFYNFCSRNKDKLTALERWKIKRIQFGFHKKDSEAGDGSSEQGAEEVEGEKKNLSDVNLSAYQAWKLKHQKKVGSENKEEVVELSKGDDLALVKKRQRRLELLERSRQTLEESQSMGSWEADSSAASGSIPLSAFWSAAPSVSADGDTASVLSAQSHSSHPSQALSKVGGCLASTPTTPLPNLHVGPGDTISIASIQNWIANVVSETLAQKQNEMLLSPRPLSVVSMKAAPAGSCLGDDQVSMLSGQSSSALGGCLLPQSQARPSSETQSVLSSHTMSARAEGTGSKVKGTSKPIYSLFADNIDLKELGRKEKEMQTELREKMSEYKMEKLASDNKRSSLFKKKKVKEDEDEDDDVGDRDEDTDSAIGSFRYSSRSNSQKPETDTFSSLAVSDGYGSGSRAGREKDNSINKWLSGLRTEEKSPQSDWSGSSREKCTRSSLIRETESKSSSYKFSKSRSEEQDTSSYDETNSNSVKSTSRFSASSTREGREMHTFSRSMFSETSSSREESPEPYFFRRTPEPSEGEESPEPRRPNGTRSRDWEDVEESSKSDFSEFGAKRKFTQSFMRSEEEGEKARMEKKEEGRFASGRQSRYWRSTDREEEEEMDDEAIIAAWRRRQEETRTKLQRRRED

Target ORF information:

RefSeq Version	XM_005203419.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dual specificity phosphatase 27 (putative) (DUSP27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203419.3

ORF Insert Sequence:

ATGGCAACCA GTGGGGACCC AGAAGAGGAG CAGGTGGTCC CGAGTGAAGA GGATGAAGCC 
AACGTGAGGG CTGTACAGGC CCGGTACCTC CGGAGCCCCT CCCCCAGCCA GTACTCAATG 
GTCTCAGATG CAGAAACAGA AAGCATTTTC ATGGATCCCA TTCACCTCTC CTCAGCTGTT 
GCAGCTAAAC AGATCATCAA TGAAGAACTC AAGCCACGTG GGGTCAGAGT CGAGGCAGAG 
TGTCCAGGCA TGCTGGAGTC TGCCGAGCAG TTGCTGGTGG AGGACCTGTA CAACCGCGTC 
AGGGAGAAGA TGGATGACAC CAGCCTGTAC AACACTCCCT GCATCCTGGA CCTCCAGCGG 
GCCTTGGTCC AAGACCGCCA AGAGGCTCCC TGGAATGAGG TGGATGAGGT CTGGCCCAAC 
GTCTTCATAG CAGAGAAGAG TGTGGCTGTG AACAAGGGGA GGCTGAAGAG GCTGGGAATT 
ACTCACATCC TGAATGCTGC TCACGGCACT GGTGTCTACA CGGGGCCTGA ATTCTACACT 
GGCCTGGAGA TCCAATACCT GGGTGTGGAG GTGGATGACT TTCCTGAGGT GGATATCTCC 
CAGCATTTCC GGAAGGCAGC CGAGTTCCTG GATGAAGCCC TGCTGACCTA TAGAGGGAAA 
GTCCTGGTCA GCAGTGAAAT GGGCGTCAGC CGTTCGGCTG TGCTGGTAGT AGCCTACCTG 
ATGATCTTCC ACAACATGGC CGTCCTGGAG GCCTTGATGA CTGTGCGCAA GAAGCGGGCC 
ATCTACCCCA ACGACGGCTT CCTGAAGCAG CTCCGCGAGC TCAACGAGAA GCTGATGGAG 
GAGCGCGAAG AGGACTTCAG CAGGGAGTGT GGGGAGGAGG CAGTGGAGGA GGGCGAGGAC 
GCCGGGAGCA CGGTGGCAGC CAGAGTGCAC ACCCTCACCG TGGAGGAGGA GGACGACGCT 
GCCAGTCACC TGAGTACCTC CTCCTTGGGG AGGGCCAGCC AGGCCTCCAA GCCCCTCACC 
CTTATCGATG AGGAGGAGGA GGAGAGGCTC TACGAGGCGT GGAAAAAGGG GCAGAGCCTC 
CCCACGGGCA AGGTCCTGCA GGGCGAGGAC GACAGGTTCT CAGCCTCCTC TCGTCAAGGT 
GAGGAGCCCG AGGATGAGGA TGTGGAGCGA ATCATCCGGG AGTGGCAAAG CCGAAATGAG 
AGGTACCAGG CAGAAGGGCA CCGGAGGTGG AATAGGGAGG AGGAAGAGGA AGAGGAGAGC 
AATGGCAGCT CCTTCGTGAG GAGAAGAAGG AGGCACACGC TGAGTGAGAG CAGCACCTCC 
GAGAGTGTGA GCAGCCATGA CATCCGGGTC CTGAAGCAGC AGCTGCAGAT GAGCAGCCAG 
AACCGAGGTG GGAGACGCCG CTCTGACTCC ATGTCCACGG AGAGCACCTG GGACATGTGG 
GACCAGAGGC TGCAGGAGAT TGAGAAGGAG GCTTCCCGAA AGTACCACTC CAGGAACAAG 
AGAGAGGAGG TGGACGCCAG CTCGGAGGTG GGGAGCAGGG CGCGGGAGGA TGACGAGGAG 
AGTGTGGCCT CCGAGGCCAG CTCCTTCTAT AACTTCTGCA GCAGAAACAA GGACAAACTC 
ACCGCCCTGG AAAGGTGGAA GATCAAGAGA ATCCAGTTTG GATTTCACAA GAAGGACTCA 
GAGGCAGGAG ATGGCAGCAG TGAGCAAGGT GCGGAGGAGG TGGAGGGGGA GAAGAAAAAC 
CTCTCTGATG TCAATCTGTC TGCCTACCAG GCCTGGAAGC TGAAACACCA AAAGAAGGTG 
GGCAGTGAGA ACAAGGAGGA GGTGGTGGAG CTCAGCAAGG GAGATGACTT AGCCTTGGTC 
AAGAAGCGGC AGCGGAGGCT GGAGCTGCTG GAGAGAAGCA GGCAGACACT GGAGGAGAGC 
CAGTCAATGG GAAGCTGGGA GGCAGATAGC TCAGCTGCCA GTGGGAGCAT CCCCCTGTCT 
GCATTCTGGT CGGCAGCCCC CTCAGTCAGC GCTGATGGGG ACACAGCATC TGTGCTCAGC 
GCCCAGAGCC ACAGCTCCCA TCCCTCTCAG GCCCTAAGCA AAGTAGGCGG ATGCCTGGCT 
TCCACCCCCA CCACACCTCT ACCTAACCTG CATGTGGGGC CTGGAGACAC AATCTCCATT 
GCCAGCATAC AGAACTGGAT TGCCAACGTA GTCAGTGAAA CTCTCGCCCA GAAGCAAAAT 
GAAATGCTGT TGTCGCCCCG CCCACTGTCC GTTGTAAGCA TGAAGGCAGC ACCAGCAGGC 
AGCTGCCTAG GGGATGACCA AGTCTCCATG CTCAGTGGAC AGAGCAGCTC TGCCCTGGGT 
GGCTGCCTGC TGCCTCAAAG CCAGGCAAGA CCCAGCTCTG AGACCCAGTC TGTGTTGTCC 
TCCCATACCA TGAGTGCAAG AGCTGAAGGT ACTGGGAGCA AAGTGAAGGG GACCAGCAAG 
CCTATCTATA GCCTCTTTGC TGACAATATT GACCTAAAGG AGCTTGGCCG GAAGGAGAAG 
GAGATGCAAA CAGAGCTGAG AGAGAAGATG TCCGAGTACA AAATGGAGAA GCTGGCCTCC 
GACAACAAAC GCAGCTCCCT TTTCAAGAAG AAGAAGGTCA AGGAAGATGA GGATGAGGAT 
GATGACGTGG GTGATAGGGA TGAGGACACT GACAGCGCCA TAGGGAGCTT CCGGTATTCT 
TCCCGCAGTA ATTCCCAGAA ACCCGAAACA GACACCTTCT CCTCCCTGGC TGTCTCTGAT 
GGCTACGGAA GTGGCAGCAG AGCTGGCAGA GAGAAGGATA ACAGTATTAA TAAGTGGCTC 
AGTGGCCTCA GGACAGAGGA AAAATCTCCC CAAAGTGATT GGTCTGGAAG CTCCAGGGAA 
AAGTGCACCA GATCTTCCCT GATTCGGGAG ACAGAGTCTA AGTCTTCCAG TTACAAGTTC 
TCCAAATCCC GGTCAGAGGA GCAGGACACC TCCTCCTACG ACGAGACCAA TAGCAACTCG 
GTAAAAAGCA CTTCACGGTT CTCTGCTTCC TCCACCAGGG AAGGCAGAGA GATGCACACA 
TTCTCCAGGT CCATGTTTAG TGAGACCTCA AGCTCCCGGG AGGAAAGCCC GGAGCCCTAC 
TTTTTCCGCC GGACCCCCGA GCCCTCTGAA GGAGAAGAGT CCCCAGAACC ACGGCGTCCA 
AATGGGACCA GATCCAGGGA CTGGGAAGAT GTGGAAGAGT CATCCAAGTC AGACTTTTCT 
GAATTTGGAG CCAAGAGGAA ATTCACCCAG AGTTTCATGA GATCTGAAGA GGAGGGAGAG 
AAAGCGAGGA TGGAAAAGAA AGAAGAAGGG AGGTTTGCTT CAGGGCGGCA GTCCCGGTAT 
TGGAGAAGCA CTGACAGGGA GGAGGAGGAG GAAATGGATG ATGAAGCCAT CATTGCTGCC 
TGGAGACGCC GGCAAGAAGA AACAAGGACT AAGCTGCAGA GAAGAAGGGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUSP27 ( XM_010802713.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801015.1 Bos taurus dual specificity phosphatase 27 (putative) (DUSP27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa58679
Clone ID Related Accession (Same CDS sequence)	XM_010802713.2 , XM_024983987.1
Accession Version	XM_010802713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	inactive dual specificity phosphatase 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103855.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTCAG ATGCAGAAAC AGAAAGCATT TTCATGGATC CCATTCACCT CTCCTCAGCT 
GTTGCAGCTA AACAGATCAT CAATGAAGAA CTCAAGCCAC GTGGGGTCAG AGTCGAGGCA 
GAGTGTCCAG GCATGCTGGA GTCTGCCGAG CAGTTGCTGG TGGAGGACCT GTACAACCGC 
GTCAGGGAGA AGATGGATGA CACCAGCCTG TACAACACTC CCTGCATCCT GGACCTCCAG 
CGGGCCTTGG TCCAAGACCG CCAAGAGGCT CCCTGGAATG AGGTGGATGA GGTCTGGCCC 
AACGTCTTCA TAGCAGAGAA GAGTGTGGCT GTGAACAAGG GGAGGCTGAA GAGGCTGGGA 
ATTACTCACA TCCTGAATGC TGCTCACGGC ACTGGTGTCT ACACGGGGCC TGAATTCTAC 
ACTGGCCTGG AGATCCAATA CCTGGGTGTG GAGGTGGATG ACTTTCCTGA GGTGGATATC 
TCCCAGCATT TCCGGAAGGC AGCCGAGTTC CTGGATGAAG CCCTGCTGAC CTATAGAGGG 
AAAGTCCTGG TCAGCAGTGA AATGGGCGTC AGCCGTTCGG CTGTGCTGGT AGTAGCCTAC 
CTGATGATCT TCCACAACAT GGCCGTCCTG GAGGCCTTGA TGACTGTGCG CAAGAAGCGG 
GCCATCTACC CCAACGACGG CTTCCTGAAG CAGCTCCGCG AGCTCAACGA GAAGCTGATG 
GAGGAGCGCG AAGAGGACTT CAGCAGGGAG TGTGGGGAGG AGGCAGTGGA GGAGGGCGAG 
GACGCCGGGA GCACGGTGGC AGCCAGAGTG CACACCCTCA CCGTGGAGGA GGAGGACGAC 
GCTGCCAGTC ACCTGAGTAC CTCCTCCTTG GGGAGGGCCA GCCAGGCCTC CAAGCCCCTC 
ACCCTTATCG ATGAGGAGGA GGAGGAGAGG CTCTACGAGG CGTGGAAAAA GGGGCAGAGC 
CTCCCCACGG GCAAGGTCCT GCAGGGCGAG GACGACAGGT TCTCAGCCTC CTCTCGTCAA 
GGTGAGGAGC CCGAGGATGA GGATGTGGAG CGAATCATCC GGGAGTGGCA AAGCCGAAAT 
GAGAGGTACC AGGCAGAAGG GCACCGGAGG TGGAATAGGG AGGAGGAAGA GGAAGAGGAG 
AGCAATGGCA GCTCCTTCGT GAGGAGAAGA AGGAGGCACA CGCTGAGTGA GAGCAGCACC 
TCCGAGAGTG TGAGCAGCCA TGACATCCGG GTCCTGAAGC AGCAGCTGCA GATGAGCAGC 
CAGAACCGAG GTGGGAGACG CCGCTCTGAC TCCATGTCCA CGGAGAGCAC CTGGGACATG 
TGGGACCAGA GGCTGCAGGA GATTGAGAAG GAGGCTTCCC GAAAGTACCA CTCCAGGAAC 
AAGAGAGAGG AGGTGGACGC CAGCTCGGAG GTGGGGAGCA GGGCGCGGGA GGATGACGAG 
GAGAGTGTGG CCTCCGAGGC CAGCTCCTTC TATAACTTCT GCAGCAGAAA CAAGGACAAA 
CTCACCGCCC TGGAAAGGTG GAAGATCAAG AGAATCCAGT TTGGATTTCA CAAGAAGGAC 
TCAGAGGCAG GAGATGGCAG CAGTGAGCAA GGTGCGGAGG AGGTGGAGGG GGAGAAGAAA 
AACCTCTCTG ATGTCAATCT GTCTGCCTAC CAGGCCTGGA AGCTGAAACA CCAAAAGAAG 
GTGGGCAGTG AGAACAAGGA GGAGGTGGTG GAGCTCAGCA AGGGAGATGA CTTAGCCTTG 
GTCAAGAAGC GGCAGCGGAG GCTGGAGCTG CTGGAGAGAA GCAGGCAGAC ACTGGAGGAG 
AGCCAGTCAA TGGGAAGCTG GGAGGCAGAT AGCTCAGCTG CCAGTGGGAG CATCCCCCTG 
TCTGCATTCT GGTCGGCAGC CCCCTCAGTC AGCGCTGATG GGGACACAGC ATCTGTGCTC 
AGCGCCCAGA GCCACAGCTC CCATCCCTCT CAGGCCCTAA GCAAAGTAGG CGGATGCCTG 
GCTTCCACCC CCACCACACC TCTACCTAAC CTGCATGTGG GGCCTGGAGA CACAATCTCC 
ATTGCCAGCA TACAGAACTG GATTGCCAAC GTAGTCAGTG AAACTCTCGC CCAGAAGCAA 
AATGAAATGC TGTTGTCGCC CCGCCCACTG TCCGTTGTAA GCATGAAGGC AGCACCAGCA 
GGCAGCTGCC TAGGGGATGA CCAAGTCTCC ATGCTCAGTG GACAGAGCAG CTCTGCCCTG 
GGTGGCTGCC TGCTGCCTCA AAGCCAGGCA AGACCCAGCT CTGAGACCCA GTCTGTGTTG 
TCCTCCCATA CCATGAGTGC AAGAGCTGAA GGTACTGGGA GCAAAGTGAA GGGGACCAGC 
AAGCCTATCT ATAGCCTCTT TGCTGACAAT ATTGACCTAA AGGAGCTTGG CCGGAAGGAG 
AAGGAGATGC AAACAGAGCT GAGAGAGAAG ATGTCCGAGT ACAAAATGGA GAAGCTGGCC 
TCCGACAACA AACGCAGCTC CCTTTTCAAG AAGAAGAAGG TCAAGGAAGA TGAGGATGAG 
GATGATGACG TGGGTGATAG GGATGAGGAC ACTGACAGCG CCATAGGGAG CTTCCGGTAT 
TCTTCCCGCA GTAATTCCCA GAAACCCGAA ACAGACACCT TCTCCTCCCT GGCTGTCTCT 
GATGGCTACG GAAGTGGCAG CAGAGCTGGC AGAGAGAAGG ATAACAGTAT TAATAAGTGG 
CTCAGTGGCC TCAGGACAGA GGAAAAATCT CCCCAAAGTG ATTGGTCTGG AAGCTCCAGG 
GAAAAGTGCA CCAGATCTTC CCTGATTCGG GAGACAGAGT CTAAGTCTTC CAGTTACAAG 
TTCTCCAAAT CCCGGTCAGA GGAGCAGGAC ACCTCCTCCT ACGACGAGAC CAATAGCAAC 
TCGGTAAAAA GCACTTCACG GTTCTCTGCT TCCTCCACCA GGGAAGGCAG AGAGATGCAC 
ACATTCTCCA GGTCCATGTT TAGTGAGACC TCAAGCTCCC GGGAGGAAAG CCCGGAGCCC 
TACTTTTTCC GCCGGACCCC CGAGCCCTCT GAAGGAGAAG AGTCCCCAGA ACCACGGCGT 
CCAAATGGGA CCAGATCCAG GGACTGGGAA GATGTGGAAG AGTCATCCAA GTCAGACTTT 
TCTGAATTTG GAGCCAAGAG GAAATTCACC CAGAGTTTCA TGAGATCTGA AGAGGAGGGA 
GAGAAAGCGA GGATGGAAAA GAAAGAAGAA GGGAGGTTTG CTTCAGGGCG GCAGTCCCGG 
TATTGGAGAA GCACTGACAG GGAGGAGGAG GAGGAAATGG ATGATGAAGC CATCATTGCT 
GCCTGGAGAC GCCGGCAAGA AGAAACAAGG ACTAAGCTGC AGAGAAGAAG GGAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801015.1
CDS	1196..4555
Translation	MVSDAETESIFMDPIHLSSAVAAKQIINEELKPRGVRVEAECPGMLESAEQLLVEDLYNRVREKMDDTSLYNTPCILDLQRALVQDRQEAPWNEVDEVWPNVFIAEKSVAVNKGRLKRLGITHILNAAHGTGVYTGPEFYTGLEIQYLGVEVDDFPEVDISQHFRKAAEFLDEALLTYRGKVLVSSEMGVSRSAVLVVAYLMIFHNMAVLEALMTVRKKRAIYPNDGFLKQLRELNEKLMEEREEDFSRECGEEAVEEGEDAGSTVAARVHTLTVEEEDDAASHLSTSSLGRASQASKPLTLIDEEEEERLYEAWKKGQSLPTGKVLQGEDDRFSASSRQGEEPEDEDVERIIREWQSRNERYQAEGHRRWNREEEEEEESNGSSFVRRRRRHTLSESSTSESVSSHDIRVLKQQLQMSSQNRGGRRRSDSMSTESTWDMWDQRLQEIEKEASRKYHSRNKREEVDASSEVGSRAREDDEESVASEASSFYNFCSRNKDKLTALERWKIKRIQFGFHKKDSEAGDGSSEQGAEEVEGEKKNLSDVNLSAYQAWKLKHQKKVGSENKEEVVELSKGDDLALVKKRQRRLELLERSRQTLEESQSMGSWEADSSAASGSIPLSAFWSAAPSVSADGDTASVLSAQSHSSHPSQALSKVGGCLASTPTTPLPNLHVGPGDTISIASIQNWIANVVSETLAQKQNEMLLSPRPLSVVSMKAAPAGSCLGDDQVSMLSGQSSSALGGCLLPQSQARPSSETQSVLSSHTMSARAEGTGSKVKGTSKPIYSLFADNIDLKELGRKEKEMQTELREKMSEYKMEKLASDNKRSSLFKKKKVKEDEDEDDDVGDRDEDTDSAIGSFRYSSRSNSQKPETDTFSSLAVSDGYGSGSRAGREKDNSINKWLSGLRTEEKSPQSDWSGSSREKCTRSSLIRETESKSSSYKFSKSRSEEQDTSSYDETNSNSVKSTSRFSASSTREGREMHTFSRSMFSETSSSREESPEPYFFRRTPEPSEGEESPEPRRPNGTRSRDWEDVEESSKSDFSEFGAKRKFTQSFMRSEEEGEKARMEKKEEGRFASGRQSRYWRSTDREEEEEMDDEAIIAAWRRRQEETRTKLQRRRED

Target ORF information:

RefSeq Version	XM_010802713.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dual specificity phosphatase 27 (putative) (DUSP27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010802713.2

ORF Insert Sequence:

ATGGTCTCAG ATGCAGAAAC AGAAAGCATT TTCATGGATC CCATTCACCT CTCCTCAGCT 
GTTGCAGCTA AACAGATCAT CAATGAAGAA CTCAAGCCAC GTGGGGTCAG AGTCGAGGCA 
GAGTGTCCAG GCATGCTGGA GTCTGCCGAG CAGTTGCTGG TGGAGGACCT GTACAACCGC 
GTCAGGGAGA AGATGGATGA CACCAGCCTG TACAACACTC CCTGCATCCT GGACCTCCAG 
CGGGCCTTGG TCCAAGACCG CCAAGAGGCT CCCTGGAATG AGGTGGATGA GGTCTGGCCC 
AACGTCTTCA TAGCAGAGAA GAGTGTGGCT GTGAACAAGG GGAGGCTGAA GAGGCTGGGA 
ATTACTCACA TCCTGAATGC TGCTCACGGC ACTGGTGTCT ACACGGGGCC TGAATTCTAC 
ACTGGCCTGG AGATCCAATA CCTGGGTGTG GAGGTGGATG ACTTTCCTGA GGTGGATATC 
TCCCAGCATT TCCGGAAGGC AGCCGAGTTC CTGGATGAAG CCCTGCTGAC CTATAGAGGG 
AAAGTCCTGG TCAGCAGTGA AATGGGCGTC AGCCGTTCGG CTGTGCTGGT AGTAGCCTAC 
CTGATGATCT TCCACAACAT GGCCGTCCTG GAGGCCTTGA TGACTGTGCG CAAGAAGCGG 
GCCATCTACC CCAACGACGG CTTCCTGAAG CAGCTCCGCG AGCTCAACGA GAAGCTGATG 
GAGGAGCGCG AAGAGGACTT CAGCAGGGAG TGTGGGGAGG AGGCAGTGGA GGAGGGCGAG 
GACGCCGGGA GCACGGTGGC AGCCAGAGTG CACACCCTCA CCGTGGAGGA GGAGGACGAC 
GCTGCCAGTC ACCTGAGTAC CTCCTCCTTG GGGAGGGCCA GCCAGGCCTC CAAGCCCCTC 
ACCCTTATCG ATGAGGAGGA GGAGGAGAGG CTCTACGAGG CGTGGAAAAA GGGGCAGAGC 
CTCCCCACGG GCAAGGTCCT GCAGGGCGAG GACGACAGGT TCTCAGCCTC CTCTCGTCAA 
GGTGAGGAGC CCGAGGATGA GGATGTGGAG CGAATCATCC GGGAGTGGCA AAGCCGAAAT 
GAGAGGTACC AGGCAGAAGG GCACCGGAGG TGGAATAGGG AGGAGGAAGA GGAAGAGGAG 
AGCAATGGCA GCTCCTTCGT GAGGAGAAGA AGGAGGCACA CGCTGAGTGA GAGCAGCACC 
TCCGAGAGTG TGAGCAGCCA TGACATCCGG GTCCTGAAGC AGCAGCTGCA GATGAGCAGC 
CAGAACCGAG GTGGGAGACG CCGCTCTGAC TCCATGTCCA CGGAGAGCAC CTGGGACATG 
TGGGACCAGA GGCTGCAGGA GATTGAGAAG GAGGCTTCCC GAAAGTACCA CTCCAGGAAC 
AAGAGAGAGG AGGTGGACGC CAGCTCGGAG GTGGGGAGCA GGGCGCGGGA GGATGACGAG 
GAGAGTGTGG CCTCCGAGGC CAGCTCCTTC TATAACTTCT GCAGCAGAAA CAAGGACAAA 
CTCACCGCCC TGGAAAGGTG GAAGATCAAG AGAATCCAGT TTGGATTTCA CAAGAAGGAC 
TCAGAGGCAG GAGATGGCAG CAGTGAGCAA GGTGCGGAGG AGGTGGAGGG GGAGAAGAAA 
AACCTCTCTG ATGTCAATCT GTCTGCCTAC CAGGCCTGGA AGCTGAAACA CCAAAAGAAG 
GTGGGCAGTG AGAACAAGGA GGAGGTGGTG GAGCTCAGCA AGGGAGATGA CTTAGCCTTG 
GTCAAGAAGC GGCAGCGGAG GCTGGAGCTG CTGGAGAGAA GCAGGCAGAC ACTGGAGGAG 
AGCCAGTCAA TGGGAAGCTG GGAGGCAGAT AGCTCAGCTG CCAGTGGGAG CATCCCCCTG 
TCTGCATTCT GGTCGGCAGC CCCCTCAGTC AGCGCTGATG GGGACACAGC ATCTGTGCTC 
AGCGCCCAGA GCCACAGCTC CCATCCCTCT CAGGCCCTAA GCAAAGTAGG CGGATGCCTG 
GCTTCCACCC CCACCACACC TCTACCTAAC CTGCATGTGG GGCCTGGAGA CACAATCTCC 
ATTGCCAGCA TACAGAACTG GATTGCCAAC GTAGTCAGTG AAACTCTCGC CCAGAAGCAA 
AATGAAATGC TGTTGTCGCC CCGCCCACTG TCCGTTGTAA GCATGAAGGC AGCACCAGCA 
GGCAGCTGCC TAGGGGATGA CCAAGTCTCC ATGCTCAGTG GACAGAGCAG CTCTGCCCTG 
GGTGGCTGCC TGCTGCCTCA AAGCCAGGCA AGACCCAGCT CTGAGACCCA GTCTGTGTTG 
TCCTCCCATA CCATGAGTGC AAGAGCTGAA GGTACTGGGA GCAAAGTGAA GGGGACCAGC 
AAGCCTATCT ATAGCCTCTT TGCTGACAAT ATTGACCTAA AGGAGCTTGG CCGGAAGGAG 
AAGGAGATGC AAACAGAGCT GAGAGAGAAG ATGTCCGAGT ACAAAATGGA GAAGCTGGCC 
TCCGACAACA AACGCAGCTC CCTTTTCAAG AAGAAGAAGG TCAAGGAAGA TGAGGATGAG 
GATGATGACG TGGGTGATAG GGATGAGGAC ACTGACAGCG CCATAGGGAG CTTCCGGTAT 
TCTTCCCGCA GTAATTCCCA GAAACCCGAA ACAGACACCT TCTCCTCCCT GGCTGTCTCT 
GATGGCTACG GAAGTGGCAG CAGAGCTGGC AGAGAGAAGG ATAACAGTAT TAATAAGTGG 
CTCAGTGGCC TCAGGACAGA GGAAAAATCT CCCCAAAGTG ATTGGTCTGG AAGCTCCAGG 
GAAAAGTGCA CCAGATCTTC CCTGATTCGG GAGACAGAGT CTAAGTCTTC CAGTTACAAG 
TTCTCCAAAT CCCGGTCAGA GGAGCAGGAC ACCTCCTCCT ACGACGAGAC CAATAGCAAC 
TCGGTAAAAA GCACTTCACG GTTCTCTGCT TCCTCCACCA GGGAAGGCAG AGAGATGCAC 
ACATTCTCCA GGTCCATGTT TAGTGAGACC TCAAGCTCCC GGGAGGAAAG CCCGGAGCCC 
TACTTTTTCC GCCGGACCCC CGAGCCCTCT GAAGGAGAAG AGTCCCCAGA ACCACGGCGT 
CCAAATGGGA CCAGATCCAG GGACTGGGAA GATGTGGAAG AGTCATCCAA GTCAGACTTT 
TCTGAATTTG GAGCCAAGAG GAAATTCACC CAGAGTTTCA TGAGATCTGA AGAGGAGGGA 
GAGAAAGCGA GGATGGAAAA GAAAGAAGAA GGGAGGTTTG CTTCAGGGCG GCAGTCCCGG 
TATTGGAGAA GCACTGACAG GGAGGAGGAG GAGGAAATGG ATGATGAAGC CATCATTGCT 
GCCTGGAGAC GCCGGCAAGA AGAAACAAGG ACTAAGCTGC AGAGAAGAAG GGAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUSP27 ( XM_024983987.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839755.1 Bos taurus dual specificity phosphatase 27, atypical (DUSP27), transcript variant X2, mRNA.

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CloneID	OBa58679
Clone ID Related Accession (Same CDS sequence)	XM_010802713.2 , XM_024983987.1
Accession Version	XM_024983987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	inactive dual specificity phosphatase 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTCAG ATGCAGAAAC AGAAAGCATT TTCATGGATC CCATTCACCT CTCCTCAGCT 
GTTGCAGCTA AACAGATCAT CAATGAAGAA CTCAAGCCAC GTGGGGTCAG AGTCGAGGCA 
GAGTGTCCAG GCATGCTGGA GTCTGCCGAG CAGTTGCTGG TGGAGGACCT GTACAACCGC 
GTCAGGGAGA AGATGGATGA CACCAGCCTG TACAACACTC CCTGCATCCT GGACCTCCAG 
CGGGCCTTGG TCCAAGACCG CCAAGAGGCT CCCTGGAATG AGGTGGATGA GGTCTGGCCC 
AACGTCTTCA TAGCAGAGAA GAGTGTGGCT GTGAACAAGG GGAGGCTGAA GAGGCTGGGA 
ATTACTCACA TCCTGAATGC TGCTCACGGC ACTGGTGTCT ACACGGGGCC TGAATTCTAC 
ACTGGCCTGG AGATCCAATA CCTGGGTGTG GAGGTGGATG ACTTTCCTGA GGTGGATATC 
TCCCAGCATT TCCGGAAGGC AGCCGAGTTC CTGGATGAAG CCCTGCTGAC CTATAGAGGG 
AAAGTCCTGG TCAGCAGTGA AATGGGCGTC AGCCGTTCGG CTGTGCTGGT AGTAGCCTAC 
CTGATGATCT TCCACAACAT GGCCGTCCTG GAGGCCTTGA TGACTGTGCG CAAGAAGCGG 
GCCATCTACC CCAACGACGG CTTCCTGAAG CAGCTCCGCG AGCTCAACGA GAAGCTGATG 
GAGGAGCGCG AAGAGGACTT CAGCAGGGAG TGTGGGGAGG AGGCAGTGGA GGAGGGCGAG 
GACGCCGGGA GCACGGTGGC AGCCAGAGTG CACACCCTCA CCGTGGAGGA GGAGGACGAC 
GCTGCCAGTC ACCTGAGTAC CTCCTCCTTG GGGAGGGCCA GCCAGGCCTC CAAGCCCCTC 
ACCCTTATCG ATGAGGAGGA GGAGGAGAGG CTCTACGAGG CGTGGAAAAA GGGGCAGAGC 
CTCCCCACGG GCAAGGTCCT GCAGGGCGAG GACGACAGGT TCTCAGCCTC CTCTCGTCAA 
GGTGAGGAGC CCGAGGATGA GGATGTGGAG CGAATCATCC GGGAGTGGCA AAGCCGAAAT 
GAGAGGTACC AGGCAGAAGG GCACCGGAGG TGGAATAGGG AGGAGGAAGA GGAAGAGGAG 
AGCAATGGCA GCTCCTTCGT GAGGAGAAGA AGGAGGCACA CGCTGAGTGA GAGCAGCACC 
TCCGAGAGTG TGAGCAGCCA TGACATCCGG GTCCTGAAGC AGCAGCTGCA GATGAGCAGC 
CAGAACCGAG GTGGGAGACG CCGCTCTGAC TCCATGTCCA CGGAGAGCAC CTGGGACATG 
TGGGACCAGA GGCTGCAGGA GATTGAGAAG GAGGCTTCCC GAAAGTACCA CTCCAGGAAC 
AAGAGAGAGG AGGTGGACGC CAGCTCGGAG GTGGGGAGCA GGGCGCGGGA GGATGACGAG 
GAGAGTGTGG CCTCCGAGGC CAGCTCCTTC TATAACTTCT GCAGCAGAAA CAAGGACAAA 
CTCACCGCCC TGGAAAGGTG GAAGATCAAG AGAATCCAGT TTGGATTTCA CAAGAAGGAC 
TCAGAGGCAG GAGATGGCAG CAGTGAGCAA GGTGCGGAGG AGGTGGAGGG GGAGAAGAAA 
AACCTCTCTG ATGTCAATCT GTCTGCCTAC CAGGCCTGGA AGCTGAAACA CCAAAAGAAG 
GTGGGCAGTG AGAACAAGGA GGAGGTGGTG GAGCTCAGCA AGGGAGATGA CTTAGCCTTG 
GTCAAGAAGC GGCAGCGGAG GCTGGAGCTG CTGGAGAGAA GCAGGCAGAC ACTGGAGGAG 
AGCCAGTCAA TGGGAAGCTG GGAGGCAGAT AGCTCAGCTG CCAGTGGGAG CATCCCCCTG 
TCTGCATTCT GGTCGGCAGC CCCCTCAGTC AGCGCTGATG GGGACACAGC ATCTGTGCTC 
AGCGCCCAGA GCCACAGCTC CCATCCCTCT CAGGCCCTAA GCAAAGTAGG CGGATGCCTG 
GCTTCCACCC CCACCACACC TCTACCTAAC CTGCATGTGG GGCCTGGAGA CACAATCTCC 
ATTGCCAGCA TACAGAACTG GATTGCCAAC GTAGTCAGTG AAACTCTCGC CCAGAAGCAA 
AATGAAATGC TGTTGTCGCC CCGCCCACTG TCCGTTGTAA GCATGAAGGC AGCACCAGCA 
GGCAGCTGCC TAGGGGATGA CCAAGTCTCC ATGCTCAGTG GACAGAGCAG CTCTGCCCTG 
GGTGGCTGCC TGCTGCCTCA AAGCCAGGCA AGACCCAGCT CTGAGACCCA GTCTGTGTTG 
TCCTCCCATA CCATGAGTGC AAGAGCTGAA GGTACTGGGA GCAAAGTGAA GGGGACCAGC 
AAGCCTATCT ATAGCCTCTT TGCTGACAAT ATTGACCTAA AGGAGCTTGG CCGGAAGGAG 
AAGGAGATGC AAACAGAGCT GAGAGAGAAG ATGTCCGAGT ACAAAATGGA GAAGCTGGCC 
TCCGACAACA AACGCAGCTC CCTTTTCAAG AAGAAGAAGG TCAAGGAAGA TGAGGATGAG 
GATGATGACG TGGGTGATAG GGATGAGGAC ACTGACAGCG CCATAGGGAG CTTCCGGTAT 
TCTTCCCGCA GTAATTCCCA GAAACCCGAA ACAGACACCT TCTCCTCCCT GGCTGTCTCT 
GATGGCTACG GAAGTGGCAG CAGAGCTGGC AGAGAGAAGG ATAACAGTAT TAATAAGTGG 
CTCAGTGGCC TCAGGACAGA GGAAAAATCT CCCCAAAGTG ATTGGTCTGG AAGCTCCAGG 
GAAAAGTGCA CCAGATCTTC CCTGATTCGG GAGACAGAGT CTAAGTCTTC CAGTTACAAG 
TTCTCCAAAT CCCGGTCAGA GGAGCAGGAC ACCTCCTCCT ACGACGAGAC CAATAGCAAC 
TCGGTAAAAA GCACTTCACG GTTCTCTGCT TCCTCCACCA GGGAAGGCAG AGAGATGCAC 
ACATTCTCCA GGTCCATGTT TAGTGAGACC TCAAGCTCCC GGGAGGAAAG CCCGGAGCCC 
TACTTTTTCC GCCGGACCCC CGAGCCCTCT GAAGGAGAAG AGTCCCCAGA ACCACGGCGT 
CCAAATGGGA CCAGATCCAG GGACTGGGAA GATGTGGAAG AGTCATCCAA GTCAGACTTT 
TCTGAATTTG GAGCCAAGAG GAAATTCACC CAGAGTTTCA TGAGATCTGA AGAGGAGGGA 
GAGAAAGCGA GGATGGAAAA GAAAGAAGAA GGGAGGTTTG CTTCAGGGCG GCAGTCCCGG 
TATTGGAGAA GCACTGACAG GGAGGAGGAG GAGGAAATGG ATGATGAAGC CATCATTGCT 
GCCTGGAGAC GCCGGCAAGA AGAAACAAGG ACTAAGCTGC AGAGAAGAAG GGAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839755.1
CDS	1062..4421
Translation	MVSDAETESIFMDPIHLSSAVAAKQIINEELKPRGVRVEAECPGMLESAEQLLVEDLYNRVREKMDDTSLYNTPCILDLQRALVQDRQEAPWNEVDEVWPNVFIAEKSVAVNKGRLKRLGITHILNAAHGTGVYTGPEFYTGLEIQYLGVEVDDFPEVDISQHFRKAAEFLDEALLTYRGKVLVSSEMGVSRSAVLVVAYLMIFHNMAVLEALMTVRKKRAIYPNDGFLKQLRELNEKLMEEREEDFSRECGEEAVEEGEDAGSTVAARVHTLTVEEEDDAASHLSTSSLGRASQASKPLTLIDEEEEERLYEAWKKGQSLPTGKVLQGEDDRFSASSRQGEEPEDEDVERIIREWQSRNERYQAEGHRRWNREEEEEEESNGSSFVRRRRRHTLSESSTSESVSSHDIRVLKQQLQMSSQNRGGRRRSDSMSTESTWDMWDQRLQEIEKEASRKYHSRNKREEVDASSEVGSRAREDDEESVASEASSFYNFCSRNKDKLTALERWKIKRIQFGFHKKDSEAGDGSSEQGAEEVEGEKKNLSDVNLSAYQAWKLKHQKKVGSENKEEVVELSKGDDLALVKKRQRRLELLERSRQTLEESQSMGSWEADSSAASGSIPLSAFWSAAPSVSADGDTASVLSAQSHSSHPSQALSKVGGCLASTPTTPLPNLHVGPGDTISIASIQNWIANVVSETLAQKQNEMLLSPRPLSVVSMKAAPAGSCLGDDQVSMLSGQSSSALGGCLLPQSQARPSSETQSVLSSHTMSARAEGTGSKVKGTSKPIYSLFADNIDLKELGRKEKEMQTELREKMSEYKMEKLASDNKRSSLFKKKKVKEDEDEDDDVGDRDEDTDSAIGSFRYSSRSNSQKPETDTFSSLAVSDGYGSGSRAGREKDNSINKWLSGLRTEEKSPQSDWSGSSREKCTRSSLIRETESKSSSYKFSKSRSEEQDTSSYDETNSNSVKSTSRFSASSTREGREMHTFSRSMFSETSSSREESPEPYFFRRTPEPSEGEESPEPRRPNGTRSRDWEDVEESSKSDFSEFGAKRKFTQSFMRSEEEGEKARMEKKEEGRFASGRQSRYWRSTDREEEEEMDDEAIIAAWRRRQEETRTKLQRRRED

Target ORF information:

RefSeq Version	XM_024983987.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dual specificity phosphatase 27, atypical (DUSP27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983987.1

ORF Insert Sequence:

ATGGTCTCAG ATGCAGAAAC AGAAAGCATT TTCATGGATC CCATTCACCT CTCCTCAGCT 
GTTGCAGCTA AACAGATCAT CAATGAAGAA CTCAAGCCAC GTGGGGTCAG AGTCGAGGCA 
GAGTGTCCAG GCATGCTGGA GTCTGCCGAG CAGTTGCTGG TGGAGGACCT GTACAACCGC 
GTCAGGGAGA AGATGGATGA CACCAGCCTG TACAACACTC CCTGCATCCT GGACCTCCAG 
CGGGCCTTGG TCCAAGACCG CCAAGAGGCT CCCTGGAATG AGGTGGATGA GGTCTGGCCC 
AACGTCTTCA TAGCAGAGAA GAGTGTGGCT GTGAACAAGG GGAGGCTGAA GAGGCTGGGA 
ATTACTCACA TCCTGAATGC TGCTCACGGC ACTGGTGTCT ACACGGGGCC TGAATTCTAC 
ACTGGCCTGG AGATCCAATA CCTGGGTGTG GAGGTGGATG ACTTTCCTGA GGTGGATATC 
TCCCAGCATT TCCGGAAGGC AGCCGAGTTC CTGGATGAAG CCCTGCTGAC CTATAGAGGG 
AAAGTCCTGG TCAGCAGTGA AATGGGCGTC AGCCGTTCGG CTGTGCTGGT AGTAGCCTAC 
CTGATGATCT TCCACAACAT GGCCGTCCTG GAGGCCTTGA TGACTGTGCG CAAGAAGCGG 
GCCATCTACC CCAACGACGG CTTCCTGAAG CAGCTCCGCG AGCTCAACGA GAAGCTGATG 
GAGGAGCGCG AAGAGGACTT CAGCAGGGAG TGTGGGGAGG AGGCAGTGGA GGAGGGCGAG 
GACGCCGGGA GCACGGTGGC AGCCAGAGTG CACACCCTCA CCGTGGAGGA GGAGGACGAC 
GCTGCCAGTC ACCTGAGTAC CTCCTCCTTG GGGAGGGCCA GCCAGGCCTC CAAGCCCCTC 
ACCCTTATCG ATGAGGAGGA GGAGGAGAGG CTCTACGAGG CGTGGAAAAA GGGGCAGAGC 
CTCCCCACGG GCAAGGTCCT GCAGGGCGAG GACGACAGGT TCTCAGCCTC CTCTCGTCAA 
GGTGAGGAGC CCGAGGATGA GGATGTGGAG CGAATCATCC GGGAGTGGCA AAGCCGAAAT 
GAGAGGTACC AGGCAGAAGG GCACCGGAGG TGGAATAGGG AGGAGGAAGA GGAAGAGGAG 
AGCAATGGCA GCTCCTTCGT GAGGAGAAGA AGGAGGCACA CGCTGAGTGA GAGCAGCACC 
TCCGAGAGTG TGAGCAGCCA TGACATCCGG GTCCTGAAGC AGCAGCTGCA GATGAGCAGC 
CAGAACCGAG GTGGGAGACG CCGCTCTGAC TCCATGTCCA CGGAGAGCAC CTGGGACATG 
TGGGACCAGA GGCTGCAGGA GATTGAGAAG GAGGCTTCCC GAAAGTACCA CTCCAGGAAC 
AAGAGAGAGG AGGTGGACGC CAGCTCGGAG GTGGGGAGCA GGGCGCGGGA GGATGACGAG 
GAGAGTGTGG CCTCCGAGGC CAGCTCCTTC TATAACTTCT GCAGCAGAAA CAAGGACAAA 
CTCACCGCCC TGGAAAGGTG GAAGATCAAG AGAATCCAGT TTGGATTTCA CAAGAAGGAC 
TCAGAGGCAG GAGATGGCAG CAGTGAGCAA GGTGCGGAGG AGGTGGAGGG GGAGAAGAAA 
AACCTCTCTG ATGTCAATCT GTCTGCCTAC CAGGCCTGGA AGCTGAAACA CCAAAAGAAG 
GTGGGCAGTG AGAACAAGGA GGAGGTGGTG GAGCTCAGCA AGGGAGATGA CTTAGCCTTG 
GTCAAGAAGC GGCAGCGGAG GCTGGAGCTG CTGGAGAGAA GCAGGCAGAC ACTGGAGGAG 
AGCCAGTCAA TGGGAAGCTG GGAGGCAGAT AGCTCAGCTG CCAGTGGGAG CATCCCCCTG 
TCTGCATTCT GGTCGGCAGC CCCCTCAGTC AGCGCTGATG GGGACACAGC ATCTGTGCTC 
AGCGCCCAGA GCCACAGCTC CCATCCCTCT CAGGCCCTAA GCAAAGTAGG CGGATGCCTG 
GCTTCCACCC CCACCACACC TCTACCTAAC CTGCATGTGG GGCCTGGAGA CACAATCTCC 
ATTGCCAGCA TACAGAACTG GATTGCCAAC GTAGTCAGTG AAACTCTCGC CCAGAAGCAA 
AATGAAATGC TGTTGTCGCC CCGCCCACTG TCCGTTGTAA GCATGAAGGC AGCACCAGCA 
GGCAGCTGCC TAGGGGATGA CCAAGTCTCC ATGCTCAGTG GACAGAGCAG CTCTGCCCTG 
GGTGGCTGCC TGCTGCCTCA AAGCCAGGCA AGACCCAGCT CTGAGACCCA GTCTGTGTTG 
TCCTCCCATA CCATGAGTGC AAGAGCTGAA GGTACTGGGA GCAAAGTGAA GGGGACCAGC 
AAGCCTATCT ATAGCCTCTT TGCTGACAAT ATTGACCTAA AGGAGCTTGG CCGGAAGGAG 
AAGGAGATGC AAACAGAGCT GAGAGAGAAG ATGTCCGAGT ACAAAATGGA GAAGCTGGCC 
TCCGACAACA AACGCAGCTC CCTTTTCAAG AAGAAGAAGG TCAAGGAAGA TGAGGATGAG 
GATGATGACG TGGGTGATAG GGATGAGGAC ACTGACAGCG CCATAGGGAG CTTCCGGTAT 
TCTTCCCGCA GTAATTCCCA GAAACCCGAA ACAGACACCT TCTCCTCCCT GGCTGTCTCT 
GATGGCTACG GAAGTGGCAG CAGAGCTGGC AGAGAGAAGG ATAACAGTAT TAATAAGTGG 
CTCAGTGGCC TCAGGACAGA GGAAAAATCT CCCCAAAGTG ATTGGTCTGG AAGCTCCAGG 
GAAAAGTGCA CCAGATCTTC CCTGATTCGG GAGACAGAGT CTAAGTCTTC CAGTTACAAG 
TTCTCCAAAT CCCGGTCAGA GGAGCAGGAC ACCTCCTCCT ACGACGAGAC CAATAGCAAC 
TCGGTAAAAA GCACTTCACG GTTCTCTGCT TCCTCCACCA GGGAAGGCAG AGAGATGCAC 
ACATTCTCCA GGTCCATGTT TAGTGAGACC TCAAGCTCCC GGGAGGAAAG CCCGGAGCCC 
TACTTTTTCC GCCGGACCCC CGAGCCCTCT GAAGGAGAAG AGTCCCCAGA ACCACGGCGT 
CCAAATGGGA CCAGATCCAG GGACTGGGAA GATGTGGAAG AGTCATCCAA GTCAGACTTT 
TCTGAATTTG GAGCCAAGAG GAAATTCACC CAGAGTTTCA TGAGATCTGA AGAGGAGGGA 
GAGAAAGCGA GGATGGAAAA GAAAGAAGAA GGGAGGTTTG CTTCAGGGCG GCAGTCCCGG 
TATTGGAGAA GCACTGACAG GGAGGAGGAG GAGGAAATGG ATGATGAAGC CATCATTGCT 
GCCTGGAGAC GCCGGCAAGA AGAAACAAGG ACTAAGCTGC AGAGAAGAAG GGAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DUSP27 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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