LOC100650259 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100650259
Entrez Gene ID	100650259
Full Name	WASH complex subunit 4
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003401729.3	XP_003401777.1	WASH complex subunit 4

Pathways from BioSystems
Gene Ontology

The following LOC100650259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100650259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo64552	XM_003401729.3 Latest version!	Bombus terrestris WASH complex subunit 4 (LOC100650259), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100650259 ( XM_003401729.3 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_003401777.1 Bombus terrestris WASH complex subunit 4 (LOC100650259), mRNA.

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CloneID	OBo64552
Clone ID Related Accession (Same CDS sequence)	XM_003401729.3
Accession Version	XM_003401729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	WASH complex subunit 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015777.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_003401729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGAAT CATCAGCTTG GAATTCGAAA AAGGACGAGA CCATATACAA AGCGGCAGGT 
TCGATACACT TACGAAAATA TGGACAGTTT TTTGAGAATT TAGCTAAGCA AACATGGAAA 
AAAGATTCTA CATTAGAATA TTTTATGGAA GGTCCTCTTC GGCTTGTATA CAAACCTGTT 
GAGGATATCA GCTTGATAAA TTTAATAGAA ATGGAAAATA AATATTTATC AAAGCTACTT 
GCTGCAGTTG CCGGTACCTG CAGAGAAATT AGACTTCTTG TGATGGAAGC TAAGATGTTC 
TATAAAAAAT TGTTTACTCA TGGTGAAAGA GGGGCAGAGA ATAATCAGAA TAATATCACA 
TCTCTTTTGT CTGATCTGCA AGATCTATTT GTTTTTGTGA ATAGGATATG GACAGTAGCA 
CATTTGACAA CGGAACAGTT ATCTAGTCTA GCAGGTGACT CTAATGATAT TTATTTACCT 
GTATTAATAG AACATTTTAT TGATTTGTTT ATAATCGTTT TGACATTGGA TGAAATTATT 
GAATCCCAGC CTTCATTGTT AGAACAGTGG AAAAAGTACA GAATGAATGT GCGTTCTATC 
ATGCATAATC CATTGCAATT TGGCATAAGT GAATCTAAAT TACATACACT GAATAAGTTA 
TTAAAAGATT TACAAGAGAA TTTATTAAAG AAAGTTTTAT TTTTTAAGAC GATTGAACGC 
ATAATGGATG TGAATAAAGG ACCTATAATG AGTGAACAGA TTACCAATTA TTTGAAAAAT 
TTAATGGTAG ACATAGAAAA TAAACCAAAT GATTATGTTT CATTGAATAA AAGTTGGATC 
AAAATAAATA TTGGAATTGT TGTAACGACA AAATTATTTG GCACCTGTGA TAAAAGATTA 
ATTAAAAGAG TGCTTGAAAA TAATAAAAAG TTTTATGCTG TAAATTTAAT TGGAAATGTC 
ATATGGATTC CCAGTAAATT TTTAAGTGAT TTTTTGCCAA AAGAAGCTGG TAATGCTGTT 
AGTCAACAAG TAGGAGAAAA AATACTATGT TTGAGGATAC AAAAACTTTC CCAGACTGTG 
AATGGCTTAA TTCAAAAAGC AGTAACATGG TGCATTGAAA TGCAAAGTAT TTTAATGAAA 
ACAAGTCTTC AGATTTCAGA TATTGGGCAG AAACAAACTT TATTAATTGA TTTAGTTGCA 
TTGTTATCTC AGATTAAAGA AATTGTATCT TTTATAATAA ATATGCATGC GATTCTTATT 
AAACCAATGA ATCGTAACAC TGTTCAATTA ATTTGTAGAT TAATAGAAGT ACAAAAGTCT 
TTGCAGACTA CATTTTACAT ATTGGGACCA GTTATAGTGC AATCACAAAG TCAAGTATTA 
CAATACTTGA GTTATTATAT TTTAATTACA TTGGAAACTG CTCGGAAGAG TTTAATTCAG 
AAGGATAAAG GTTATAGCAG AGAAAAACTG GATGCATTGT CACTAGTGGG TTTAAGTATG 
AGATTATTAA ATGGACCACC AAGTGCAGAT AGAAGATTAA TAATCAGATG TGCTCTAGCA 
TGTGCTAGTC AATTAACAGA TACTTTTAAG GAAGAGGACA TGGTGAAATT GAGATTTTCA 
TTGAACAATT ACGATATTGT TGCTGAATTG TATAATATTA TTACAGATAT CTGTGATTAC 
TCTATATTAT TATATCATCA GAATATAATT CCAGCTTATT TTTCTTCTGT AATAGATAGT 
AATTTAAGTA TAAATCACAT AGTTCATTTG TTTAATGCAT TTAACAGCAC CATTAACGAA 
CAAGAAGGAT TAAATTTAAA ACAGAAAAGA ATTATGCAAT TAAGAGAAAT GTTGACTAAG 
AATATATTAG AACCTGTTTG TCATGAGATT GAAACTAATC TAAGACTTCA TGTCCATGCA 
CATTTAAAAT TGGATTCTAC AAATCCATTC AATATTGGGG CAAAAGATGG TGGAAGAATC 
GTACGATCAT TACCACTACC TTTAACAAAT AGTATGATAT ACGCTAAAAG ATTCATTGAA 
CATTACCTTG ATGATATGTT TTACAATCTC ACTACAGTAG CATTACATGA TTGGAGAACA 
TATAGAATGA TGCATGCTTT GGCACATTAT AAATTAAATC TAGATACTGT ACAAAACTAT 
TTACCTACAC AGACCTTGGA ACAGGGCTTA GATGCTTTAG AAATTATGAG AAATATTCAT 
GTATTTGTAT CAAAATATTT ATATAATTTA AACACTCAAA CTTTTATTGA ACATACTAGT 
AACAATAAAC ATTTAAATAC TATTGGAATT CGACATATAG CAAATTCTAT CAGAACACAT 
GGAACTGGTA TTATGAGTAC AACAGTTAAC TTTGTATATC AATTTCTTCG TATAAAATTA 
CATACATTTT CACAATTCCT TTTTGATGAA CATATAAAAT CGAGATTAAT GCGAGATATA 
AGGTTCATCA AGGTTCAAAG AGAAAATGGT AGAACACCTT ATACATATGA AAGAGCAGAA 
AAATTTCAGA AAGGGATCAG AAAATTAGGT ATGACACCTG ATGGTTTAAG TTATTTAGAT 
CAATTTCGAC AATTAATAAC TCAAATTGGA AATGCATTGG GGTATGTACG ATTAATTAGA 
TCTGGTGGAT TGCATGCTAG TTCAAATGCT ATTTCATTTC TGCCAAACAT CGATTCATCT 
GTACCATTTG AATTAATGTG TAAAAATTTA AGTTATGATG TAACAACTCA ATCAGCAGCA 
AAGTGTTTAG AAAATAATAT TGCAAATTTA ATACGAAACT TTACAGAAGG AAGAATACAA 
TACTTCAAAC TTCTTGTTGA TGTATTTGCA TCTGCTTTTC GAGATACTGA ATCGCATCAT 
TTGCAACAAT TTTATGCTAT TGTACCTCCA TTAACATTAA GTTTTATTGA TAACTCAATA 
TCGAATAAAG AAAAAATGTT TAAGAAAAAT CAAACAGGTG CAGCTTTTAC TGATGATGGT 
TTTGCAATGG GAATTGCTTA TATAAATGCT CTTTTAAATC AGTCATCAGA ATTGGATAGT 
TTACATTGGT TCAAAACAGT TGAACAGCAT ATAAATGCTG AACAGCAGGC TGCAGAAAGT 
AAAAATGTTC AAGGAGATGA AAAATTGCAA CAAACAAGAG CATTAACATT AAAACGCTTA 
GGTGAAAGAA GTGCTGAATT CCAATTGCTC TATTATAGTT TGTCTGGCGC TAGAGTATTC 
TTTAAACAGT CTGATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003401777.1
CDS	294..3554
Translation	MIESSAWNSKKDETIYKAAGSIHLRKYGQFFENLAKQTWKKDSTLEYFMEGPLRLVYKPVEDISLINLIEMENKYLSKLLAAVAGTCREIRLLVMEAKMFYKKLFTHGERGAENNQNNITSLLSDLQDLFVFVNRIWTVAHLTTEQLSSLAGDSNDIYLPVLIEHFIDLFIIVLTLDEIIESQPSLLEQWKKYRMNVRSIMHNPLQFGISESKLHTLNKLLKDLQENLLKKVLFFKTIERIMDVNKGPIMSEQITNYLKNLMVDIENKPNDYVSLNKSWIKINIGIVVTTKLFGTCDKRLIKRVLENNKKFYAVNLIGNVIWIPSKFLSDFLPKEAGNAVSQQVGEKILCLRIQKLSQTVNGLIQKAVTWCIEMQSILMKTSLQISDIGQKQTLLIDLVALLSQIKEIVSFIINMHAILIKPMNRNTVQLICRLIEVQKSLQTTFYILGPVIVQSQSQVLQYLSYYILITLETARKSLIQKDKGYSREKLDALSLVGLSMRLLNGPPSADRRLIIRCALACASQLTDTFKEEDMVKLRFSLNNYDIVAELYNIITDICDYSILLYHQNIIPAYFSSVIDSNLSINHIVHLFNAFNSTINEQEGLNLKQKRIMQLREMLTKNILEPVCHEIETNLRLHVHAHLKLDSTNPFNIGAKDGGRIVRSLPLPLTNSMIYAKRFIEHYLDDMFYNLTTVALHDWRTYRMMHALAHYKLNLDTVQNYLPTQTLEQGLDALEIMRNIHVFVSKYLYNLNTQTFIEHTSNNKHLNTIGIRHIANSIRTHGTGIMSTTVNFVYQFLRIKLHTFSQFLFDEHIKSRLMRDIRFIKVQRENGRTPYTYERAEKFQKGIRKLGMTPDGLSYLDQFRQLITQIGNALGYVRLIRSGGLHASSNAISFLPNIDSSVPFELMCKNLSYDVTTQSAAKCLENNIANLIRNFTEGRIQYFKLLVDVFASAFRDTESHHLQQFYAIVPPLTLSFIDNSISNKEKMFKKNQTGAAFTDDGFAMGIAYINALLNQSSELDSLHWFKTVEQHINAEQQAAESKNVQGDEKLQQTRALTLKRLGERSAEFQLLYYSLSGARVFFKQSDM

Target ORF information:

RefSeq Version	XM_003401729.3
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris WASH complex subunit 4 (LOC100650259), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003401729.3

ORF Insert Sequence:

ATGATAGAAT CATCAGCTTG GAATTCGAAA AAGGACGAGA CCATATACAA AGCGGCAGGT 
TCGATACACT TACGAAAATA TGGACAGTTT TTTGAGAATT TAGCTAAGCA AACATGGAAA 
AAAGATTCTA CATTAGAATA TTTTATGGAA GGTCCTCTTC GGCTTGTATA CAAACCTGTT 
GAGGATATCA GCTTGATAAA TTTAATAGAA ATGGAAAATA AATATTTATC AAAGCTACTT 
GCTGCAGTTG CCGGTACCTG CAGAGAAATT AGACTTCTTG TGATGGAAGC TAAGATGTTC 
TATAAAAAAT TGTTTACTCA TGGTGAAAGA GGGGCAGAGA ATAATCAGAA TAATATCACA 
TCTCTTTTGT CTGATCTGCA AGATCTATTT GTTTTTGTGA ATAGGATATG GACAGTAGCA 
CATTTGACAA CGGAACAGTT ATCTAGTCTA GCAGGTGACT CTAATGATAT TTATTTACCT 
GTATTAATAG AACATTTTAT TGATTTGTTT ATAATCGTTT TGACATTGGA TGAAATTATT 
GAATCCCAGC CTTCATTGTT AGAACAGTGG AAAAAGTACA GAATGAATGT GCGTTCTATC 
ATGCATAATC CATTGCAATT TGGCATAAGT GAATCTAAAT TACATACACT GAATAAGTTA 
TTAAAAGATT TACAAGAGAA TTTATTAAAG AAAGTTTTAT TTTTTAAGAC GATTGAACGC 
ATAATGGATG TGAATAAAGG ACCTATAATG AGTGAACAGA TTACCAATTA TTTGAAAAAT 
TTAATGGTAG ACATAGAAAA TAAACCAAAT GATTATGTTT CATTGAATAA AAGTTGGATC 
AAAATAAATA TTGGAATTGT TGTAACGACA AAATTATTTG GCACCTGTGA TAAAAGATTA 
ATTAAAAGAG TGCTTGAAAA TAATAAAAAG TTTTATGCTG TAAATTTAAT TGGAAATGTC 
ATATGGATTC CCAGTAAATT TTTAAGTGAT TTTTTGCCAA AAGAAGCTGG TAATGCTGTT 
AGTCAACAAG TAGGAGAAAA AATACTATGT TTGAGGATAC AAAAACTTTC CCAGACTGTG 
AATGGCTTAA TTCAAAAAGC AGTAACATGG TGCATTGAAA TGCAAAGTAT TTTAATGAAA 
ACAAGTCTTC AGATTTCAGA TATTGGGCAG AAACAAACTT TATTAATTGA TTTAGTTGCA 
TTGTTATCTC AGATTAAAGA AATTGTATCT TTTATAATAA ATATGCATGC GATTCTTATT 
AAACCAATGA ATCGTAACAC TGTTCAATTA ATTTGTAGAT TAATAGAAGT ACAAAAGTCT 
TTGCAGACTA CATTTTACAT ATTGGGACCA GTTATAGTGC AATCACAAAG TCAAGTATTA 
CAATACTTGA GTTATTATAT TTTAATTACA TTGGAAACTG CTCGGAAGAG TTTAATTCAG 
AAGGATAAAG GTTATAGCAG AGAAAAACTG GATGCATTGT CACTAGTGGG TTTAAGTATG 
AGATTATTAA ATGGACCACC AAGTGCAGAT AGAAGATTAA TAATCAGATG TGCTCTAGCA 
TGTGCTAGTC AATTAACAGA TACTTTTAAG GAAGAGGACA TGGTGAAATT GAGATTTTCA 
TTGAACAATT ACGATATTGT TGCTGAATTG TATAATATTA TTACAGATAT CTGTGATTAC 
TCTATATTAT TATATCATCA GAATATAATT CCAGCTTATT TTTCTTCTGT AATAGATAGT 
AATTTAAGTA TAAATCACAT AGTTCATTTG TTTAATGCAT TTAACAGCAC CATTAACGAA 
CAAGAAGGAT TAAATTTAAA ACAGAAAAGA ATTATGCAAT TAAGAGAAAT GTTGACTAAG 
AATATATTAG AACCTGTTTG TCATGAGATT GAAACTAATC TAAGACTTCA TGTCCATGCA 
CATTTAAAAT TGGATTCTAC AAATCCATTC AATATTGGGG CAAAAGATGG TGGAAGAATC 
GTACGATCAT TACCACTACC TTTAACAAAT AGTATGATAT ACGCTAAAAG ATTCATTGAA 
CATTACCTTG ATGATATGTT TTACAATCTC ACTACAGTAG CATTACATGA TTGGAGAACA 
TATAGAATGA TGCATGCTTT GGCACATTAT AAATTAAATC TAGATACTGT ACAAAACTAT 
TTACCTACAC AGACCTTGGA ACAGGGCTTA GATGCTTTAG AAATTATGAG AAATATTCAT 
GTATTTGTAT CAAAATATTT ATATAATTTA AACACTCAAA CTTTTATTGA ACATACTAGT 
AACAATAAAC ATTTAAATAC TATTGGAATT CGACATATAG CAAATTCTAT CAGAACACAT 
GGAACTGGTA TTATGAGTAC AACAGTTAAC TTTGTATATC AATTTCTTCG TATAAAATTA 
CATACATTTT CACAATTCCT TTTTGATGAA CATATAAAAT CGAGATTAAT GCGAGATATA 
AGGTTCATCA AGGTTCAAAG AGAAAATGGT AGAACACCTT ATACATATGA AAGAGCAGAA 
AAATTTCAGA AAGGGATCAG AAAATTAGGT ATGACACCTG ATGGTTTAAG TTATTTAGAT 
CAATTTCGAC AATTAATAAC TCAAATTGGA AATGCATTGG GGTATGTACG ATTAATTAGA 
TCTGGTGGAT TGCATGCTAG TTCAAATGCT ATTTCATTTC TGCCAAACAT CGATTCATCT 
GTACCATTTG AATTAATGTG TAAAAATTTA AGTTATGATG TAACAACTCA ATCAGCAGCA 
AAGTGTTTAG AAAATAATAT TGCAAATTTA ATACGAAACT TTACAGAAGG AAGAATACAA 
TACTTCAAAC TTCTTGTTGA TGTATTTGCA TCTGCTTTTC GAGATACTGA ATCGCATCAT 
TTGCAACAAT TTTATGCTAT TGTACCTCCA TTAACATTAA GTTTTATTGA TAACTCAATA 
TCGAATAAAG AAAAAATGTT TAAGAAAAAT CAAACAGGTG CAGCTTTTAC TGATGATGGT 
TTTGCAATGG GAATTGCTTA TATAAATGCT CTTTTAAATC AGTCATCAGA ATTGGATAGT 
TTACATTGGT TCAAAACAGT TGAACAGCAT ATAAATGCTG AACAGCAGGC TGCAGAAAGT 
AAAAATGTTC AAGGAGATGA AAAATTGCAA CAAACAAGAG CATTAACATT AAAACGCTTA 
GGTGAAAGAA GTGCTGAATT CCAATTGCTC TATTATAGTT TGTCTGGCGC TAGAGTATTC 
TTTAAACAGT CTGATATGTA A