LOC100646478 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100646478
Entrez Gene ID	100646478
Full Name	probable RNA methyltransferase bin3
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003399359.3	XP_003399407.1	probable RNA methyltransferase bin3

Pathways from BioSystems
Gene Ontology

The following LOC100646478 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100646478 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo60670	XM_003399359.3 Latest version!	Bombus terrestris probable RNA methyltransferase bin3 (LOC100646478), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100646478 ( XM_003399359.3 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_003399407.1 Bombus terrestris probable RNA methyltransferase bin3 (LOC100646478), transcript variant X1, mRNA.

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CloneID	OBo60670
Clone ID Related Accession (Same CDS sequence)	XM_003399359.3
Accession Version	XM_003399359.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	probable RNA methyltransferase bin3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_003399359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA CGCAAGTTGA TCGGTCCACC AAAATGGGTC ATATTACTAA GAAGGAAAAT 
GAAAAATCAA GAAAGACATT TAATTCTTCT CATAAAAAAC ACAAGCACGA AGACAGTCGT 
CATTTTAAGT TTGGTAGGAA ACGTCTACAG AGTTTTATTA GCAATGGTAA ATTCTTTCCA 
CCATATAAAC GTAGGAAGAA GGAAGGTGCA ATTATTCCAC CCACAAAATT TTTACTTGGT 
GGTAACATAC GTGATCCTTT AAATTTAAAT AGCATGCAGG ATGAGGAAAT AAATAGAGCT 
ATGAATGCTG TTACACCTAA GTCTAGCCCA TTGCCTACAC CTAGACATAA AACAGAGGTT 
ATTGAAGTAA TAATTCCACC AAATATCTGT GACCCTTTGA ATCTGAGCAA TTGTAATGAC 
AATGAGGAAT ATGAAAAACA GCTTATATCC CCAACTAAGA AATGTTCTAA GCGACGAAAT 
AGAAAGAAAA AACGGGCATC TTCTGGTTCT GGGAATGATG CAAGTGATTC AACTGAAATC 
AAAATTAAAG ACTGTGATGC TATTGATATT GTAGAACCCA TGGAACAAAG TGTTCTAGAC 
AATATTGTGG CAGAGCAACA ACAACCACCA CTGAATTCAT CACCGACTAA TTCATCCAGT 
CAGCCTAAAG AGACAAAACC AGAAAGTTCA CAGAAAGACA AGAATAAATT ACGTTTGAAA 
GGTTTAGAGG AACCTAAGGA CAAAAGGTTA AGGAAAGTGG ATGTTAAAGA TAAAATTGTC 
AGTCCTGTAA TTCCACAACC TGGAGGCTGG AAGACTAGAC CACTGCATAG GCCAAGTCAA 
GATAAGAAGA AGAAACAAAC AATGCCCAGG TTCAGAGAGA AAGATGCTCG TTATCAATAT 
GGAAATTACA ACAGGTATTA TGGGTACCGT AATTCTCATA ATGATATGGA TACAAGACTG 
ACAGTATTTG TACAGTACAA ATATTTATTT ATTGACAAAG ATGTATTAGA CATTGGTTGC 
AACATTGGTC ATATTACTCT TTCTGTTGCA AGAGATTTCT CTGCCCGCAG TGTAACTGGT 
ATAGATATTG ATAGAACACT CATCAATATA GCTCGAAAAA ATATTAAACA TTATGTCAAT 
TGTGCACAGT CTCCTGCTGG CAACGAGGAT AGTGATCACC AAGATGTAAA CTTCTTTCCA 
ATATCTATGC CAATCATTTA TGGTCCAGTT GACATTCCAG GTTTCACAAA GAATAAAAGT 
CACAGAGGTT TCCCTTACAA TGTCACTTTT GTACAGGGTA ATTATGTGCT CGAGGATGAT 
TCACTATTGT GTACAGAACA GCCGCAGTTC GACACAATTC TTTGTCTGTC GATAACTAAA 
TGGATACACT TGAATTTCGG AGATGCAGGT TTAAAACAGG CTTTCAGGCG TATGTACGCA 
CAATTACGCC CCGGTGGAGT TTTATTACTA GAACCTCAAG GTTGGAGTAG TTACTCCAAG 
AAAAAAAATC TTACAGAACG GATATATAAG AATTATGAGA GTATTGAATT TCGACCGCAT 
AACTTCACGC AGTATCTCTT ATCTTCTGAA GTCGGCTTTT CCAAATATGA AGTGTTATCG 
ATACCTCCAC ACCCATCGAA AGGATTTCAA CGGCCGATCT ATTTGTTTAC TAAAGCTGGC 
CTATCGCAAG AGAGATCTGA TAATTCTGTG AAAAACGTAA CAAAACGACA AGAACGAGAG 
ATTGAAGAGA GGAGAGAATT AGAGCAGGAG CAACAGCATG AAGGGAGAAG AGAATTGGGA 
CAGAAACAAA AAGATGAGGA GAGGGCGGAA TTACAAGAGC AACGAAATGA TGAAGAGACA 
AGAAAATTAG AAGAGGAGCG AAAGGATGAA GAGAGGAAAA AGTTCGACAA TCTAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003399407.1
CDS	259..2178
Translation	MSSTQVDRSTKMGHITKKENEKSRKTFNSSHKKHKHEDSRHFKFGRKRLQSFISNGKFFPPYKRRKKEGAIIPPTKFLLGGNIRDPLNLNSMQDEEINRAMNAVTPKSSPLPTPRHKTEVIEVIIPPNICDPLNLSNCNDNEEYEKQLISPTKKCSKRRNRKKKRASSGSGNDASDSTEIKIKDCDAIDIVEPMEQSVLDNIVAEQQQPPLNSSPTNSSSQPKETKPESSQKDKNKLRLKGLEEPKDKRLRKVDVKDKIVSPVIPQPGGWKTRPLHRPSQDKKKKQTMPRFREKDARYQYGNYNRYYGYRNSHNDMDTRLTVFVQYKYLFIDKDVLDIGCNIGHITLSVARDFSARSVTGIDIDRTLINIARKNIKHYVNCAQSPAGNEDSDHQDVNFFPISMPIIYGPVDIPGFTKNKSHRGFPYNVTFVQGNYVLEDDSLLCTEQPQFDTILCLSITKWIHLNFGDAGLKQAFRRMYAQLRPGGVLLLEPQGWSSYSKKKNLTERIYKNYESIEFRPHNFTQYLLSSEVGFSKYEVLSIPPHPSKGFQRPIYLFTKAGLSQERSDNSVKNVTKRQEREIEERRELEQEQQHEGRRELGQKQKDEERAELQEQRNDEETRKLEEERKDEERKKFDNLT

Target ORF information:

RefSeq Version	XM_003399359.3
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris probable RNA methyltransferase bin3 (LOC100646478), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003399359.3

ORF Insert Sequence:

ATGTCATCCA CGCAAGTTGA TCGGTCCACC AAAATGGGTC ATATTACTAA GAAGGAAAAT 
GAAAAATCAA GAAAGACATT TAATTCTTCT CATAAAAAAC ACAAGCACGA AGACAGTCGT 
CATTTTAAGT TTGGTAGGAA ACGTCTACAG AGTTTTATTA GCAATGGTAA ATTCTTTCCA 
CCATATAAAC GTAGGAAGAA GGAAGGTGCA ATTATTCCAC CCACAAAATT TTTACTTGGT 
GGTAACATAC GTGATCCTTT AAATTTAAAT AGCATGCAGG ATGAGGAAAT AAATAGAGCT 
ATGAATGCTG TTACACCTAA GTCTAGCCCA TTGCCTACAC CTAGACATAA AACAGAGGTT 
ATTGAAGTAA TAATTCCACC AAATATCTGT GACCCTTTGA ATCTGAGCAA TTGTAATGAC 
AATGAGGAAT ATGAAAAACA GCTTATATCC CCAACTAAGA AATGTTCTAA GCGACGAAAT 
AGAAAGAAAA AACGGGCATC TTCTGGTTCT GGGAATGATG CAAGTGATTC AACTGAAATC 
AAAATTAAAG ACTGTGATGC TATTGATATT GTAGAACCCA TGGAACAAAG TGTTCTAGAC 
AATATTGTGG CAGAGCAACA ACAACCACCA CTGAATTCAT CACCGACTAA TTCATCCAGT 
CAGCCTAAAG AGACAAAACC AGAAAGTTCA CAGAAAGACA AGAATAAATT ACGTTTGAAA 
GGTTTAGAGG AACCTAAGGA CAAAAGGTTA AGGAAAGTGG ATGTTAAAGA TAAAATTGTC 
AGTCCTGTAA TTCCACAACC TGGAGGCTGG AAGACTAGAC CACTGCATAG GCCAAGTCAA 
GATAAGAAGA AGAAACAAAC AATGCCCAGG TTCAGAGAGA AAGATGCTCG TTATCAATAT 
GGAAATTACA ACAGGTATTA TGGGTACCGT AATTCTCATA ATGATATGGA TACAAGACTG 
ACAGTATTTG TACAGTACAA ATATTTATTT ATTGACAAAG ATGTATTAGA CATTGGTTGC 
AACATTGGTC ATATTACTCT TTCTGTTGCA AGAGATTTCT CTGCCCGCAG TGTAACTGGT 
ATAGATATTG ATAGAACACT CATCAATATA GCTCGAAAAA ATATTAAACA TTATGTCAAT 
TGTGCACAGT CTCCTGCTGG CAACGAGGAT AGTGATCACC AAGATGTAAA CTTCTTTCCA 
ATATCTATGC CAATCATTTA TGGTCCAGTT GACATTCCAG GTTTCACAAA GAATAAAAGT 
CACAGAGGTT TCCCTTACAA TGTCACTTTT GTACAGGGTA ATTATGTGCT CGAGGATGAT 
TCACTATTGT GTACAGAACA GCCGCAGTTC GACACAATTC TTTGTCTGTC GATAACTAAA 
TGGATACACT TGAATTTCGG AGATGCAGGT TTAAAACAGG CTTTCAGGCG TATGTACGCA 
CAATTACGCC CCGGTGGAGT TTTATTACTA GAACCTCAAG GTTGGAGTAG TTACTCCAAG 
AAAAAAAATC TTACAGAACG GATATATAAG AATTATGAGA GTATTGAATT TCGACCGCAT 
AACTTCACGC AGTATCTCTT ATCTTCTGAA GTCGGCTTTT CCAAATATGA AGTGTTATCG 
ATACCTCCAC ACCCATCGAA AGGATTTCAA CGGCCGATCT ATTTGTTTAC TAAAGCTGGC 
CTATCGCAAG AGAGATCTGA TAATTCTGTG AAAAACGTAA CAAAACGACA AGAACGAGAG 
ATTGAAGAGA GGAGAGAATT AGAGCAGGAG CAACAGCATG AAGGGAGAAG AGAATTGGGA 
CAGAAACAAA AAGATGAGGA GAGGGCGGAA TTACAAGAGC AACGAAATGA TGAAGAGACA 
AGAAAATTAG AAGAGGAGCG AAAGGATGAA GAGAGGAAAA AGTTCGACAA TCTAACTTAA