LOC100644749 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100644749
Entrez Gene ID	100644749
Full Name	alkaline phosphatase 4
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012317050.2	XP_012172440.2	LOW QUALITY PROTEIN: alkaline phosphatase 4

Pathways from BioSystems
Gene Ontology

The following LOC100644749 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100644749 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo65807	XM_012317050.2 Latest version!	Bombus terrestris alkaline phosphatase 4 (LOC100644749), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100644749 ( XM_012317050.2 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_012172440.2 Bombus terrestris alkaline phosphatase 4 (LOC100644749), mRNA.

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CloneID	OBo65807
Clone ID Related Accession (Same CDS sequence)	XM_012317050.2
Accession Version	XM_012317050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	LOW QUALITY PROTEIN: alkaline phosphatase 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_012317050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGATCCATT TACGGATCGA GTTATTTGGA TTTACAAATT CTACGAAGAT GAGGTGTCTG 
GTTGTATTAG CGTGTTCTCT ATTGGGATTT CTCGAGCTCG CAAATTCTCT ACCTCGAAAT 
GCTACCGATT ACGAAGATAT GTCCTTTTGG CTGAAATCCG GCCAAGAGAA TCTACGAAGA 
ATTCTGGCTT ATCGAAACAA CGAAAATCGC GCGAAGAATG TAATTATCTT TATCGGTGAT 
GGCATGGGAA TATCTACGAT CACAGCTGGT CGAATATACA AAGGCCAAAT CAAGGGTAAC 
ACCGGCGAGG AATACAAATT AGCTTTTGAA AATTTTCCTA ACACTGGACT CGCCAAGACG 
TACAACACCG ATAAACAAGT ACCCGATTCA GCTGGAACAG CTACCGCGAT ATTTTCCGGT 
GTTAAATGCC GATACAAGGT TATTGGATTA GATACGAAAG CTTCGTTTAA TCGATGTAAT 
AACAAAACCG ACGGGGCGAG CAAAGTTACG ACTGTCGCAG ATTGGGCGCA GCAGAGTGGC 
ATGGATACCG GGTTTGTCAC GACAACTCGG ATCACGCATG CTACTCCGGG CGGATTATAC 
GCTCATACCA ATAATCGCGA CTGGGAATGC GATACGTCGA TACCGGCGCA GTATAAAGGT 
TGCGTAAAGG ATATCGCGAG ACAACTAATC GAAGACGAAC CAGGAAACAA GTTCAAGGTG 
ATTATGGGTG GTGGAGCGCA ACATATGGGC TTACCAATGG AGCCGATAGA TCCCGATACG 
TGTGTCAGAG GAGATGGACA AAATTTGGCG GATGTATGGG CTGAGAATAA TCCCGACGGC 
AAAGTGGTGA CTAATCTCGA ACAGCTTCTC TCCGTCGATA TCGCTAACAC GTCGAAAATT 
CTCGGAATTT TTGCTCCTAG TCATCTCCCT TACCATGCTG TGAAGACGGA GCAAACACCG 
AGCTTAGCCG AGATGACCCA GCAGGCTGTT CGGTTGCTTA AAAAANATGA CAATGGTTTT 
CTCTTAATGG TGGAGAGTGG AAGAATAGAC ATGGCGCATC ACCATAACTG GGCGAAGCTA 
GCGTTGAGGG AATTATCGGA ACTCGAAGAA GCGATATCGG TAGCTCTGCA GGAAATTAAA 
CTGGAAGAAA CGTTGGTGAT CGTAACTGCG GACCACTCGC ACGCGTTCAC CATGAATGGT 
TATCCGAAAC GTGGAAACGA TATCCTTGGT TTCGCCAACG ATCCAAACAA GCCAGAAATA 
CAGTCCTACG AAACGCTCAG CTACATCAAT GGTCCAGGAT TCTATCATCA TAGGCGAAAG 
GATAGTAACA ACATTAACGA AACATGGAAA CCTGTCGATC AAGACAAGGA ACGCGACGAA 
CCATATTACA CCCATATGGC TGGCATATAC TTGGAAGATG AAACTCACGG GGGCGAAGAC 
GTTGGAGTCT ACGCTATAGG TCCGTACTCC CACTTGATTC GTGGCACTTT CGAGCAAAAT 
TACATCGCTC ACGTAGTAGC GTATGCGGCG TGTCTCAAAA ACTGGCCTTC CCACTGTGAT 
AACTCCCACT CTTACAATTC GGTAACTCAA CCTACTAGTT CCCCTATTGT GTTTCTCTTG 
ACGTCCGTGC TGCTTTTAAT CGTCACCGGG TTCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012172440.2
CDS	442..2100
Translation	MIHLRIELFGFTNSTKMRCLVVLACSLLGFLELANSLPRNATDYEDMSFWLKSGQENLRRILAYRNNENRAKNVIIFIGDGMGISTITAGRIYKGQIKGNTGEEYKLAFENFPNTGLAKTYNTDKQVPDSAGTATAIFSGVKCRYKVIGLDTKASFNRCNNKTDGASKVTTVADWAQQSGMDTGFVTTTRITHATPGGLYAHTNNRDWECDTSIPAQYKGCVKDIARQLIEDEPGNKFKVIMGGGAQHMGLPMEPIDPDTCVRGDGQNLADVWAENNPDGKVVTNLEQLLSVDIANTSKILGIFAPSHLPYHAVKTEQTPSLAEMTQQAVRLLKKnullDNGFLLMVESGRIDMAHHHNWAKLALRELSELEEAISVALQEIKLEETLVIVTADHSHAFTMNGYPKRGNDILGFANDPNKPEIQSYETLSYINGPGFYHHRRKDSNNINETWKPVDQDKERDEPYYTHMAGIYLEDETHGGEDVGVYAIGPYSHLIRGTFEQNYIAHVVAYAACLKNWPSHCDNSHSYNSVTQPTSSPIVFLLTSVLLLIVTGFD

Target ORF information:

RefSeq Version	XM_012317050.2
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris alkaline phosphatase 4 (LOC100644749), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012317050.2

ORF Insert Sequence:

ATGATCCATT TACGGATCGA GTTATTTGGA TTTACAAATT CTACGAAGAT GAGGTGTCTG 
GTTGTATTAG CGTGTTCTCT ATTGGGATTT CTCGAGCTCG CAAATTCTCT ACCTCGAAAT 
GCTACCGATT ACGAAGATAT GTCCTTTTGG CTGAAATCCG GCCAAGAGAA TCTACGAAGA 
ATTCTGGCTT ATCGAAACAA CGAAAATCGC GCGAAGAATG TAATTATCTT TATCGGTGAT 
GGCATGGGAA TATCTACGAT CACAGCTGGT CGAATATACA AAGGCCAAAT CAAGGGTAAC 
ACCGGCGAGG AATACAAATT AGCTTTTGAA AATTTTCCTA ACACTGGACT CGCCAAGACG 
TACAACACCG ATAAACAAGT ACCCGATTCA GCTGGAACAG CTACCGCGAT ATTTTCCGGT 
GTTAAATGCC GATACAAGGT TATTGGATTA GATACGAAAG CTTCGTTTAA TCGATGTAAT 
AACAAAACCG ACGGGGCGAG CAAAGTTACG ACTGTCGCAG ATTGGGCGCA GCAGAGTGGC 
ATGGATACCG GGTTTGTCAC GACAACTCGG ATCACGCATG CTACTCCGGG CGGATTATAC 
GCTCATACCA ATAATCGCGA CTGGGAATGC GATACGTCGA TACCGGCGCA GTATAAAGGT 
TGCGTAAAGG ATATCGCGAG ACAACTAATC GAAGACGAAC CAGGAAACAA GTTCAAGGTG 
ATTATGGGTG GTGGAGCGCA ACATATGGGC TTACCAATGG AGCCGATAGA TCCCGATACG 
TGTGTCAGAG GAGATGGACA AAATTTGGCG GATGTATGGG CTGAGAATAA TCCCGACGGC 
AAAGTGGTGA CTAATCTCGA ACAGCTTCTC TCCGTCGATA TCGCTAACAC GTCGAAAATT 
CTCGGAATTT TTGCTCCTAG TCATCTCCCT TACCATGCTG TGAAGACGGA GCAAACACCG 
AGCTTAGCCG AGATGACCCA GCAGGCTGTT CGGTTGCTTA AAAAANATGA CAATGGTTTT 
CTCTTAATGG TGGAGAGTGG AAGAATAGAC ATGGCGCATC ACCATAACTG GGCGAAGCTA 
GCGTTGAGGG AATTATCGGA ACTCGAAGAA GCGATATCGG TAGCTCTGCA GGAAATTAAA 
CTGGAAGAAA CGTTGGTGAT CGTAACTGCG GACCACTCGC ACGCGTTCAC CATGAATGGT 
TATCCGAAAC GTGGAAACGA TATCCTTGGT TTCGCCAACG ATCCAAACAA GCCAGAAATA 
CAGTCCTACG AAACGCTCAG CTACATCAAT GGTCCAGGAT TCTATCATCA TAGGCGAAAG 
GATAGTAACA ACATTAACGA AACATGGAAA CCTGTCGATC AAGACAAGGA ACGCGACGAA 
CCATATTACA CCCATATGGC TGGCATATAC TTGGAAGATG AAACTCACGG GGGCGAAGAC 
GTTGGAGTCT ACGCTATAGG TCCGTACTCC CACTTGATTC GTGGCACTTT CGAGCAAAAT 
TACATCGCTC ACGTAGTAGC GTATGCGGCG TGTCTCAAAA ACTGGCCTTC CCACTGTGAT 
AACTCCCACT CTTACAATTC GGTAACTCAA CCTACTAGTT CCCCTATTGT GTTTCTCTTG 
ACGTCCGTGC TGCTTTTAAT CGTCACCGGG TTCGATTAA