PPAP2A cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	PPAP2A
Entrez Gene ID	105001072
Full Name	phospholipid phosphatase 1
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010857193.1	XP_010855495.1	lipid phosphate phosphohydrolase 1

Pathways from BioSystems
Gene Ontology

The following PPAP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPAP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi77503	XM_010857193.1 Latest version!	Bison bison bison phosphatidic acid phosphatase type 2A (PPAP2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPAP2A ( XM_010857193.1 ) cDNA ORF clone, Bison bison bison -> XP_010855495.1 Bison bison bison phosphatidic acid phosphatase type 2A (PPAP2A), mRNA.

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CloneID	OBi77503
Clone ID Related Accession (Same CDS sequence)	XM_010857193.1
Accession Version	XM_010857193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	lipid phosphate phosphohydrolase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494950.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 45% of CDS bases ##RefSeq-Attributes-END##

ATGCCGGATT TGCCGGCACC ACGGGTGCGG CCGCCCTGCT GCGCCCGTGC CGTTGGGGCA 
GAGAGGAAGC GGCTATTTCT ACGCTCAGTG CTCAGAACCG TGGGCACTAA GAATGATGCC 
GGACTTGCCG GCACCACGGG TGCGGCCGCC CTGCTGCACC CGTGCCGTTG GGGCAGAGAG 
GAAGCGGCTA TTTCTACGCT CAGTGCTCAG AACCACTATA CTTCAAGATC TGATGTGAAA 
AGACCATCAT TTGGTGTTTT TCGTGTTTCA ATGAGAGATA GCAGTAGCCA GACACCTTGC 
AGTGAACAGT CCATAGCAAG GAAGAATAGT AATGGAGCTA CAGGTGCAGC CCATGATGCT 
GATAAGCCAC AAGCACCAAC GACCGGTGGC CAGAGATCAG AAGGCCAGGT TCGGCGACAC 
CAGCAGAAAG CGTGTCTGAA CAACGTTCCT CGCACTGCAC GGGAGGTGCC CCCACGCTCC 
GCCCGTCCGC CGCGCGTCCC ACTTGAGGGG CGGGCTCCGC AGGTGACTCG CCGGGAATCG 
CGGACGGGCG TGGGGGGCGT GTGCCTGACG CGCGAACCTT GCCCGGCTAG ACGCTGGGGC 
GGCTTCCGCA GCCCGGGGAC CCTGCTTCGC AGCCCGCATC CCCGCGTGGC CGCTGCTGTC 
AGTCTGCCTT TCTATTGCCC GCCGGGCTTC TGGTCGCCAG TTGCTCTTTT GACGAAAAAG 
AATCGAAGTC ATCTGGCTGG ATTGCCTTTT GCAATTCTTA CTTCAAGGCA TACCCCCTTC 
CAACGAGGAT TATTCTGTAA TGATGAGTCC ATCAAGTACC CTTACAAAGA AGACACCATA 
CCTTACCCGT TATTAGGTGG AATAATCATT CCATTCAGTA TTATCGTTAT GATTGTTGGA 
GAAACCCTGT CTGTTTATTT TAGCCTCTTG CACTCGAATT CCTTTATCAG GAATAACTAC 
ATAGCCACTA TTTACAAAGC CATTGGAACA TTTTTATTTG GTGCAGCTGC TAGTCAGTCC 
CTGACTGATA TTGCCAAGTA TTCGATAGGC AGACTGCGGC CTCACTTCTT GGATGTTTGT 
GACCCAGACT GGTCAAAAGT CAACTGCAGT GATGGTTACA TTGAAAACTA CATATGCCGA 
GGGAATGCAC AAAAAGTGAA GGAGGGCAGA CTCTCCTTTT ATTCGGGCCA CTCTTCATTC 
TCCATGTACT GCATGATGTT CGTGGCACTT TACCTTCAAG CCAGAATGAA GGGAGACTGG 
GCAAGACTTT TACGCCCTAC ACTGCAATTT GGTCTTGTTG CTGCATCCAT TTATGTGGGC 
CTTTCTCGAG TTTCTGATTA CAAACACCAC TGGAGTGATG TGTTGACTGG ACTCATTCAA 
GGAGCTCTGG TTGCAATATT AGTTGCTGTA TATGTATCAG ATTTCTTCAA AAAGAGAAGT 
TCTCCTTTTA AAGAAAGAAA AGAGGAGGAT TCTCATACAA CTCTACACGA AACACCGACG 
ACGGGGAATC ACTATCGAAA CAGTCACCAG CCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010855495.1
CDS	1..1536
Translation	MPDLPAPRVRPPCCARAVGAERKRLFLRSVLRTVGTKNDAGLAGTTGAAALLHPCRWGREEAAISTLSAQNHYTSRSDVKRPSFGVFRVSMRDSSSQTPCSEQSIARKNSNGATGAAHDADKPQAPTTGGQRSEGQVRRHQQKACLNNVPRTAREVPPRSARPPRVPLEGRAPQVTRRESRTGVGGVCLTREPCPARRWGGFRSPGTLLRSPHPRVAAAVSLPFYCPPGFWSPVALLTKKNRSHLAGLPFAILTSRHTPFQRGLFCNDESIKYPYKEDTIPYPLLGGIIIPFSIIVMIVGETLSVYFSLLHSNSFIRNNYIATIYKAIGTFLFGAAASQSLTDIAKYSIGRLRPHFLDVCDPDWSKVNCSDGYIENYICRGNAQKVKEGRLSFYSGHSSFSMYCMMFVALYLQARMKGDWARLLRPTLQFGLVAASIYVGLSRVSDYKHHWSDVLTGLIQGALVAILVAVYVSDFFKKRSSPFKERKEEDSHTTLHETPTTGNHYRNSHQP

Target ORF information:

RefSeq Version	XM_010857193.1
Organism	Bison bison bison
Definition	Bison bison bison phosphatidic acid phosphatase type 2A (PPAP2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010857193.1

ORF Insert Sequence:

ATGCCGGATT TGCCGGCACC ACGGGTGCGG CCGCCCTGCT GCGCCCGTGC CGTTGGGGCA 
GAGAGGAAGC GGCTATTTCT ACGCTCAGTG CTCAGAACCG TGGGCACTAA GAATGATGCC 
GGACTTGCCG GCACCACGGG TGCGGCCGCC CTGCTGCACC CGTGCCGTTG GGGCAGAGAG 
GAAGCGGCTA TTTCTACGCT CAGTGCTCAG AACCACTATA CTTCAAGATC TGATGTGAAA 
AGACCATCAT TTGGTGTTTT TCGTGTTTCA ATGAGAGATA GCAGTAGCCA GACACCTTGC 
AGTGAACAGT CCATAGCAAG GAAGAATAGT AATGGAGCTA CAGGTGCAGC CCATGATGCT 
GATAAGCCAC AAGCACCAAC GACCGGTGGC CAGAGATCAG AAGGCCAGGT TCGGCGACAC 
CAGCAGAAAG CGTGTCTGAA CAACGTTCCT CGCACTGCAC GGGAGGTGCC CCCACGCTCC 
GCCCGTCCGC CGCGCGTCCC ACTTGAGGGG CGGGCTCCGC AGGTGACTCG CCGGGAATCG 
CGGACGGGCG TGGGGGGCGT GTGCCTGACG CGCGAACCTT GCCCGGCTAG ACGCTGGGGC 
GGCTTCCGCA GCCCGGGGAC CCTGCTTCGC AGCCCGCATC CCCGCGTGGC CGCTGCTGTC 
AGTCTGCCTT TCTATTGCCC GCCGGGCTTC TGGTCGCCAG TTGCTCTTTT GACGAAAAAG 
AATCGAAGTC ATCTGGCTGG ATTGCCTTTT GCAATTCTTA CTTCAAGGCA TACCCCCTTC 
CAACGAGGAT TATTCTGTAA TGATGAGTCC ATCAAGTACC CTTACAAAGA AGACACCATA 
CCTTACCCGT TATTAGGTGG AATAATCATT CCATTCAGTA TTATCGTTAT GATTGTTGGA 
GAAACCCTGT CTGTTTATTT TAGCCTCTTG CACTCGAATT CCTTTATCAG GAATAACTAC 
ATAGCCACTA TTTACAAAGC CATTGGAACA TTTTTATTTG GTGCAGCTGC TAGTCAGTCC 
CTGACTGATA TTGCCAAGTA TTCGATAGGC AGACTGCGGC CTCACTTCTT GGATGTTTGT 
GACCCAGACT GGTCAAAAGT CAACTGCAGT GATGGTTACA TTGAAAACTA CATATGCCGA 
GGGAATGCAC AAAAAGTGAA GGAGGGCAGA CTCTCCTTTT ATTCGGGCCA CTCTTCATTC 
TCCATGTACT GCATGATGTT CGTGGCACTT TACCTTCAAG CCAGAATGAA GGGAGACTGG 
GCAAGACTTT TACGCCCTAC ACTGCAATTT GGTCTTGTTG CTGCATCCAT TTATGTGGGC 
CTTTCTCGAG TTTCTGATTA CAAACACCAC TGGAGTGATG TGTTGACTGG ACTCATTCAA 
GGAGCTCTGG TTGCAATATT AGTTGCTGTA TATGTATCAG ATTTCTTCAA AAAGAGAAGT 
TCTCCTTTTA AAGAAAGAAA AGAGGAGGAT TCTCATACAA CTCTACACGA AACACCGACG 
ACGGGGAATC ACTATCGAAA CAGTCACCAG CCTTGA