LOC105224581 cDNA ORF clone, Bactrocera dorsalis(oriental fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105224581
Entrez Gene ID	105224581
Full Name	endoplasmic reticulum metallopeptidase 1
Synonyms	ERMP1
Gene Type	protein-coding
Organism	Bactrocera dorsalis(oriental fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011202710.2	XP_011201012.2	LOW QUALITY PROTEIN: endoplasmic reticulum metallopeptidase 1
XM_029549535.1	XP_029405395.1	LOW QUALITY PROTEIN: endoplasmic reticulum metallopeptidase 1

Pathways from BioSystems
Gene Ontology

The following LOC105224581 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105224581 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa124685	XM_011202710.2 Latest version!	Bactrocera dorsalis endoplasmic reticulum metallopeptidase 1 (LOC105224581), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa260114	XM_029549535.1 Latest version!	Bactrocera dorsalis endoplasmic reticulum metallopeptidase 1 (LOC105224581), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105224581 ( XM_011202710.2 ) cDNA ORF clone, Bactrocera dorsalis(oriental fruit fly) -> XP_011201012.2 Bactrocera dorsalis endoplasmic reticulum metallopeptidase 1 (LOC105224581), mRNA.

Price & Availability↑

CloneID	OBa124685
Clone ID Related Accession (Same CDS sequence)	XM_011202710.2
Accession Version	XM_011202710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-16
Organism	Bactrocera dorsalis(oriental fruit fly)
Product	LOW QUALITY PROTEIN: endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011876309.1) and transcript sequence (GAKP01018077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 17, 2017 this sequence version replaced XM_011202710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera dorsalis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases frameshifts :: corrected 1 indel assembly gap :: added 242 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTCGCCG GTTACTTTCT CACTGGCAAA ATTTTTGAAG AAATATGGAC CGATGATTCC 
AACGGCTCAA AAGCCACACC AAACTCGTTA AAGAAATTCG AAATACACAA CAACAACATA 
GAGCGAAAGA AAAGCAGAAA ATTTGATTGG TACTGGGCAC CCGTCTACTG TGCTGTCTGG 
TATGTGCTTT TCTATGCCGC CGTCATACCG AGCTTCTATA ATTATCCAAA GACGCTCCAA 
ATCAGCGAAG AACAGCAGCA CCCCAACGAA TTTATAGGAC AACGTGCCGA ACAGCAACTA 
AGCACATTAG CTGATATTGG CGTTAAACTA TCGGGGACCA TAGAAAATGA AGTTCTAGCC 
ATCAATTTTC TACTCGGCGA AATTGAAAAA GTCAAAGCAC AAGCGCGACT TGATCTCTAC 
GATATCGATG TGGAAGTGCA ATACGCTTCG GGAAAGTTCG AATTGTGGCA AATGGCTACC 
AGCTATCGCA ACGTATCCAA TGTGGTGGTG AAGTTGGCGC CAAAGAATGT TAAATCCGCT 
TCTTATTTAC TGGTGAATGC GCATTTCGAT TCAGAAGTGC ACTCGCCGGC TGCCGCAGAT 
GACGGTGTGA TGATCGTAAT TATGTTGGAG ACGCTCAGAG TGATTGCCAA ATCGGAAAAG 
CCGCTTATGC ATCCCATTGT ATTTCTCTTC AATGGCGCTG AGGAGAGCAA TATGCTGGCA 
TCGCACGGTT TCATCACACA GCACCCATGG GCGCAGTATT GCAAAGCCGT TATCAATTTG 
GACTCCGCTG GTTCGGGTGG TCGCGAAATT CTCTTTCAAT CGGGTCCCAA TCACCCTTGG 
CTCATGAAGT ACTATAAACA AAGTGTACCC CGCCCCTATG CTACCACAAT CGCTGAAGAG 
TTGTTCCAAA ATAATTTCAT ACCATCGGAT ACCGATTTTC GCATATTCCG TGACTATGGC 
GAGTTACCTG GCCTCGATAT GGCGCATTCT CTCAACGGTT ATGTTTATCA CACTAAATTC 
GATACTTTTG CGAATCTCGA AAGGCACACG TGTCAGACGA CTGGTGAGAA TGTCTTAGCG 
TTAACGTGGG CACTGGCCAA TGCACCGGAA TTGAAGGACC CAGGGGACCT AGCCAAAGGC 
CACACTGTCT TCTACGATTA TCTCGGCTGG TTTATGATCT TCTACACAGA GGATACTGGT 
GTCATACTCA ATATCACCTT CTCTGTTTGT GCCATCATAG CCATCTTAGT TGCGGTTTTT 
CTGATGTCAC GCTCTGACGA CGCCGACTCT ACCAAGCGTG TCTTCTTACG CTTTCTTACC 
ATATTGGTTG CACAAATTAT AACAATAGCT GCGGCAGTGG GTCTAGCAAT TTTAGTTGCG 
GTTACCATAG ATGGCATCGG TGCCTCAGAG TGTTGGTACT CAGAGCCATG GCTCGTCTTT 
GGCATCTACT TTTGCCCTGT CTTCTTTATT ATGTGTTTGC TGCCGGCCAT TTACATGCGT 
TGGACCAAGG AAGGAACCAA TATGCATCTT GATGACTCTG TTGCCTGCTT CATGCATTCG 
CATTGCCTCA TTTTGGTGTG CCTTTGTCTG GTTATGACTG GTTTGAGTAT ACGATCGGCT 
TTCTTTCCAA TGATTGGCAT CTTCTTCTAT ACCATATCGG TTGTATTGAA TATAATAAAT 
CACTTGTGGG GCAAGAGCTA CTTGCACTTG CTTATACATC TTCTTTGTCA GTTGCTGCCA 
TTTTGGTTTT ATACCTATCA AACATTTGTC TTTCTCACAA CATTCATACC AATGCAAGGA 
CGCAGTGGAC CAACCAGTAA ACCGGAATTT ATGATTGCTG GCATTTGTGC TATAATGGCT 
ATACATTTCG GTGGTTTCAT TCTTCCAATA TTGCATAAGT TCCGCAAATC AAAAACTTTT 
ATAAGTTTAT TTGGAGCTCT ATTTTTGATA TTCATTATGA TTGCTTGCCT GCCTGTTGGC 
TTCCCTNATA AGAAGGATGT GGCTGTACAG AGAGTTTACG TTTTGCACAC GGCACGCACA 
TTCTACGACG AGCGAGGTTT TGTCTACAAA AACGAAAGCG GTTTCTATGT GCAACCTGTC 
GATAAGCGTA TTAACTTATT GAAGAAAACC GTTTTACAGA ATGCTGAACC AAATTCGAAG 
ATTCAAGAAG ATTGTGAGAC AGAACTTTTT TGCGGGCTGC CATTATATAA TTCGCGTTGG 
ATGGATTGGA AAAACTCCTC CCGCTGGGTG GTGGCGCCAA GTCCATATCT GCCTATACCA 
ACTGAACTCA AGTTAACCTC AAAGGTTTAT ATTACTGGGA CACGAGTCCG TTATGGCTTT 
AGCTTAAAGT CCGCAGATCG CGTAGTGATC TATATAGATC CTTATGCAAA TACGAAAATT 
GTTGATTGGA CTTTTGATAA GACGCCATTG AAAAGTAATT TCTCGACACC TTACTTTGTG 
TATCACATCT ATTCGATGGA TGATAGGCCA TTGGATTTCT GGATTGAATT GGACCGCGGT 
ACTCCAGACT ATGAGGGCGC CGCTTTGCGC CTTGGCATCG GCGCACACTT TATGTACCAT 
GATGCTTACT ATACTGAAGA ATTTAAAGAC TTCCTCAAGC TATTTCCCGA GTGGTCATAT 
CCGTTAGACT GGTTAGCTTC ATATGAGAGT CGCATTTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011201012.2
CDS	1..2682
Translation	MVAGYFLTGKIFEEIWTDDSNGSKATPNSLKKFEIHNNNIERKKSRKFDWYWAPVYCAVWYVLFYAAVIPSFYNYPKTLQISEEQQHPNEFIGQRAEQQLSTLADIGVKLSGTIENEVLAINFLLGEIEKVKAQARLDLYDIDVEVQYASGKFELWQMATSYRNVSNVVVKLAPKNVKSASYLLVNAHFDSEVHSPAAADDGVMIVIMLETLRVIAKSEKPLMHPIVFLFNGAEESNMLASHGFITQHPWAQYCKAVINLDSAGSGGREILFQSGPNHPWLMKYYKQSVPRPYATTIAEELFQNNFIPSDTDFRIFRDYGELPGLDMAHSLNGYVYHTKFDTFANLERHTCQTTGENVLALTWALANAPELKDPGDLAKGHTVFYDYLGWFMIFYTEDTGVILNITFSVCAIIAILVAVFLMSRSDDADSTKRVFLRFLTILVAQIITIAAAVGLAILVAVTIDGIGASECWYSEPWLVFGIYFCPVFFIMCLLPAIYMRWTKEGTNMHLDDSVACFMHSHCLILVCLCLVMTGLSIRSAFFPMIGIFFYTISVVLNIINHLWGKSYLHLLIHLLCQLLPFWFYTYQTFVFLTTFIPMQGRSGPTSKPEFMIAGICAIMAIHFGGFILPILHKFRKSKTFISLFGALFLIFIMIACLPVGFPnullKKDVAVQRVYVLHTARTFYDERGFVYKNESGFYVQPVDKRINLLKKTVLQNAEPNSKIQEDCETELFCGLPLYNSRWMDWKNSSRWVVAPSPYLPIPTELKLTSKVYITGTRVRYGFSLKSADRVVIYIDPYANTKIVDWTFDKTPLKSNFSTPYFVYHIYSMDDRPLDFWIELDRGTPDYEGAALRLGIGAHFMYHDAYYTEEFKDFLKLFPEWSYPLDWLASYESRIF

Target ORF information:

RefSeq Version	XM_011202710.2
Organism	Bactrocera dorsalis(oriental fruit fly)
Definition	Bactrocera dorsalis endoplasmic reticulum metallopeptidase 1 (LOC105224581), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011202710.2

ORF Insert Sequence:

ATGGTCGCCG GTTACTTTCT CACTGGCAAA ATTTTTGAAG AAATATGGAC CGATGATTCC 
AACGGCTCAA AAGCCACACC AAACTCGTTA AAGAAATTCG AAATACACAA CAACAACATA 
GAGCGAAAGA AAAGCAGAAA ATTTGATTGG TACTGGGCAC CCGTCTACTG TGCTGTCTGG 
TATGTGCTTT TCTATGCCGC CGTCATACCG AGCTTCTATA ATTATCCAAA GACGCTCCAA 
ATCAGCGAAG AACAGCAGCA CCCCAACGAA TTTATAGGAC AACGTGCCGA ACAGCAACTA 
AGCACATTAG CTGATATTGG CGTTAAACTA TCGGGGACCA TAGAAAATGA AGTTCTAGCC 
ATCAATTTTC TACTCGGCGA AATTGAAAAA GTCAAAGCAC AAGCGCGACT TGATCTCTAC 
GATATCGATG TGGAAGTGCA ATACGCTTCG GGAAAGTTCG AATTGTGGCA AATGGCTACC 
AGCTATCGCA ACGTATCCAA TGTGGTGGTG AAGTTGGCGC CAAAGAATGT TAAATCCGCT 
TCTTATTTAC TGGTGAATGC GCATTTCGAT TCAGAAGTGC ACTCGCCGGC TGCCGCAGAT 
GACGGTGTGA TGATCGTAAT TATGTTGGAG ACGCTCAGAG TGATTGCCAA ATCGGAAAAG 
CCGCTTATGC ATCCCATTGT ATTTCTCTTC AATGGCGCTG AGGAGAGCAA TATGCTGGCA 
TCGCACGGTT TCATCACACA GCACCCATGG GCGCAGTATT GCAAAGCCGT TATCAATTTG 
GACTCCGCTG GTTCGGGTGG TCGCGAAATT CTCTTTCAAT CGGGTCCCAA TCACCCTTGG 
CTCATGAAGT ACTATAAACA AAGTGTACCC CGCCCCTATG CTACCACAAT CGCTGAAGAG 
TTGTTCCAAA ATAATTTCAT ACCATCGGAT ACCGATTTTC GCATATTCCG TGACTATGGC 
GAGTTACCTG GCCTCGATAT GGCGCATTCT CTCAACGGTT ATGTTTATCA CACTAAATTC 
GATACTTTTG CGAATCTCGA AAGGCACACG TGTCAGACGA CTGGTGAGAA TGTCTTAGCG 
TTAACGTGGG CACTGGCCAA TGCACCGGAA TTGAAGGACC CAGGGGACCT AGCCAAAGGC 
CACACTGTCT TCTACGATTA TCTCGGCTGG TTTATGATCT TCTACACAGA GGATACTGGT 
GTCATACTCA ATATCACCTT CTCTGTTTGT GCCATCATAG CCATCTTAGT TGCGGTTTTT 
CTGATGTCAC GCTCTGACGA CGCCGACTCT ACCAAGCGTG TCTTCTTACG CTTTCTTACC 
ATATTGGTTG CACAAATTAT AACAATAGCT GCGGCAGTGG GTCTAGCAAT TTTAGTTGCG 
GTTACCATAG ATGGCATCGG TGCCTCAGAG TGTTGGTACT CAGAGCCATG GCTCGTCTTT 
GGCATCTACT TTTGCCCTGT CTTCTTTATT ATGTGTTTGC TGCCGGCCAT TTACATGCGT 
TGGACCAAGG AAGGAACCAA TATGCATCTT GATGACTCTG TTGCCTGCTT CATGCATTCG 
CATTGCCTCA TTTTGGTGTG CCTTTGTCTG GTTATGACTG GTTTGAGTAT ACGATCGGCT 
TTCTTTCCAA TGATTGGCAT CTTCTTCTAT ACCATATCGG TTGTATTGAA TATAATAAAT 
CACTTGTGGG GCAAGAGCTA CTTGCACTTG CTTATACATC TTCTTTGTCA GTTGCTGCCA 
TTTTGGTTTT ATACCTATCA AACATTTGTC TTTCTCACAA CATTCATACC AATGCAAGGA 
CGCAGTGGAC CAACCAGTAA ACCGGAATTT ATGATTGCTG GCATTTGTGC TATAATGGCT 
ATACATTTCG GTGGTTTCAT TCTTCCAATA TTGCATAAGT TCCGCAAATC AAAAACTTTT 
ATAAGTTTAT TTGGAGCTCT ATTTTTGATA TTCATTATGA TTGCTTGCCT GCCTGTTGGC 
TTCCCTNATA AGAAGGATGT GGCTGTACAG AGAGTTTACG TTTTGCACAC GGCACGCACA 
TTCTACGACG AGCGAGGTTT TGTCTACAAA AACGAAAGCG GTTTCTATGT GCAACCTGTC 
GATAAGCGTA TTAACTTATT GAAGAAAACC GTTTTACAGA ATGCTGAACC AAATTCGAAG 
ATTCAAGAAG ATTGTGAGAC AGAACTTTTT TGCGGGCTGC CATTATATAA TTCGCGTTGG 
ATGGATTGGA AAAACTCCTC CCGCTGGGTG GTGGCGCCAA GTCCATATCT GCCTATACCA 
ACTGAACTCA AGTTAACCTC AAAGGTTTAT ATTACTGGGA CACGAGTCCG TTATGGCTTT 
AGCTTAAAGT CCGCAGATCG CGTAGTGATC TATATAGATC CTTATGCAAA TACGAAAATT 
GTTGATTGGA CTTTTGATAA GACGCCATTG AAAAGTAATT TCTCGACACC TTACTTTGTG 
TATCACATCT ATTCGATGGA TGATAGGCCA TTGGATTTCT GGATTGAATT GGACCGCGGT 
ACTCCAGACT ATGAGGGCGC CGCTTTGCGC CTTGGCATCG GCGCACACTT TATGTACCAT 
GATGCTTACT ATACTGAAGA ATTTAAAGAC TTCCTCAAGC TATTTCCCGA GTGGTCATAT 
CCGTTAGACT GGTTAGCTTC ATATGAGAGT CGCATTTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105224581 ( XM_029549535.1 ) cDNA ORF clone, Bactrocera dorsalis(oriental fruit fly) -> XP_029405395.1 Bactrocera dorsalis endoplasmic reticulum metallopeptidase 1 (LOC105224581), mRNA.

Price & Availability↑

CloneID	OBa260114
Clone ID Related Accession (Same CDS sequence)	XM_029549535.1
Accession Version	XM_029549535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Bactrocera dorsalis(oriental fruit fly)
Product	LOW QUALITY PROTEIN: endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011876309.1) and transcript sequence (GGBS01009052.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bactrocera dorsalis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel assembly gap :: added 347 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGCGTTA AGAAATTCGA AATACACAAC AACAACATAG AGCGAAAGAA AAGCAGAAAA 
TTTGATTGGT ACTGGGCACC CGTCTACTGT GCTGTCTGGT ATGTGCTTTT CTATGCCGCC 
GTCATACCGA GCTTCTATAA TTATCCAAAG ACGCTCCAAA TCAGCGAAGA ACAGCAGCAC 
CCCAACGAAT TTATAGGACA ACGTGCCGAA CAGCAACTAA GCACATTAGC TGATATTGGC 
GTTAAACTAT CGGGGACCAT AGAAAATGAA GTTCTAGCCA TCAATTTTCT ACTCGGCGAA 
ATTGAAAAAG TCAAAGCACA AGCGCGACTT GATCTCTACG ATATCGATGT GGAAGTGCAA 
TACGCTTCGG GAAAGTTCGA ATTGTGGCAA ATGGCTACCA GCTATCGCAA CGTATCCAAT 
GTGGTGGTGA AGTTGGCGCC AAAGAATGTT AAATCCGCTT CTTATTTACT GGTGAATGCG 
CATTTCGATT CAGAAGTGCA CTCGCCGGCT GCCGCAGATG ACGGTGTGAT GATCGTAATT 
ATGTTGGAGA CGCTCAGAGT GATTGCCAAA TCGGAAAAGC CGCTTATGCA TCCCATTGTA 
TTTCTCTTCA ATGGCGCTGA GGAGAGCAAT ATGCTGGCAT CGCACGGTTT CATCACACAG 
CACCCATGGG CGCAGTATTG CAAAGCCGTT ATCAATTTGG ACTCCGCTGG TTCGGGTGGT 
CGCGAAATTC TCTTTCAATC GGGTCCCAAT CACCCTTGGC TCATGAAGTA CTATAAACAA 
AGTGTACCCC GCCCCTATGC TACCACAATC GCTGAAGAGT TGTTCCAAAA TAATTTCATA 
CCATCGGATA CCGATTTTCG CATATTCCGT GACTATGGCG AGTTACCTGG CCTCGATATG 
GCGCATTCTC TCAACGGTTA TGTTTATCAC ACTAAATTCG ATACTTTTGC GAATCTCGAA 
AGGCACACGT GTCAGACGAC TGGTGAGAAT GTCTTAGCGT TAACGTGGGC ACTGGCCAAT 
GCACCGGAAT TGAAGGACCC AGGGGACCTA GCCAAAGGCC ACACTGTCTT CTACGATTAT 
CTCGGCTGGT TTATGATCTT CTACACAGAG GATACTGGTG TCATACTCAA TATCACCTTC 
TCTGTTTGTG CCATCATAGC CATCTTAGTT GCGGTTTTTC TGATGTCACG CTCTGACGAC 
GCCGACTCTA CCAAGCGTGT CTTCTTACGC TTTCTTACCA TATTGGTTGC ACAAATTATA 
ACAATAGCTG CGGCAGTGGG TCTAGCAATT TTAGTTGCGG TTACCATAGA TGGCATCGGT 
GCCTCAGAGT GTTGGTACTC AGAGCCATGG CTCGTCTTTG GCATCTACTT TTGCCCTGTC 
TTCTTTATTA TGTGTTTGCT GCCGGCCATT TACATGCGTT GGACCAAGGA AGGAACCAAT 
ATGCATCTTG ATGACTCTGT TGCCTGCTTC ATGCATTCGC ATTGCCTCAT TTTGGTGTGC 
CTTTGTCTGG TTATGACTGG TTTGAGTATA CGATCGGCTT TCTTTCCAAT GATTGGCATC 
TTCTTCTATA CCATATCGGT TGTATTGAAT ATAATAAATC ACTTGTGGGG CAAGAGCTAC 
TTGCACTTGC TTATACATCT TCTTTGTCAG TTGCTGCCAT TTTGGTTTTA TACCTATCAA 
ACATTTGTCT TTCTCACAAC ATTCATACCA ATGCAAGGAC GCAGTGGACC AACCAGTAAA 
CCGGAATTTA TGATTGCTGG CATTTGTGCT ATAATGGCTA TACATTTCGG TGGTTTCATT 
CTTCCAATAT TGCATAAGTT CCGCAAATCA AAAACTTTTA TAAGTTTATT TGGAGCTCTA 
TTTTTGATAT TCATTATGAT TGCTTGCCTG CCTGTTGGCT TCCCTATANA GAAGGATGTG 
GCTGTACAGA GAGTTTACGT TTTGCACACG GCACGCACAT TCTACGACGA GCGAGGTTTT 
GTCTACAAAA ACGAAAGCGG TTTCTATGTG CAACCTGTCG ATAAGCGTAT TAACTTATTG 
AAGAAAACCG TTTTACAGAA TGCTGAACCA AATTCGAAGA TTCAAGAAGA TTGTGAGACA 
GAACTTTTTT GCGGGCTGCC ATTATATAAT TCGCGTTGGA TGGATTGGAA AAACTCCTCC 
CGCTGGGTGG TGGCGCCAAG TCCATATCTG CCTATACCAA CTGAACTCAA GTTAACCTCA 
AAGGTTTATA TTACTGGGAC ACGAGTCCGT TATGGCTTTA GCTTAAAGTC CGCAGATCGC 
GTAGTGATCT ATATAGATCC TTATGCAAAT ACGAAAATTG TTGATTGGAC TTTTGATAAG 
ACGCCATTGA AAAGTAATTT CTCGACACCT TACTTTGTGT ATCACATCTA TTCGATGGAT 
GATAGGCCAT TGGATTTCTG GATTGAATTG GACCGCGGTA CTCCAGACTA TGAGGGCGCC 
GCTTTGCGTC TTGGCATCGG CGCACACTTT ATGTACCATG ATGCCTACTA TACTGAAGAA 
TTTAAAGACT TCCTCAAGCT ATTTCCCGAG TGGTCATATC CGTTAGACTG GTTAGCTTCC 
TATGAGAGTC GCATTTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029405395.1
CDS	113..2713
Translation	MSVKKFEIHNNNIERKKSRKFDWYWAPVYCAVWYVLFYAAVIPSFYNYPKTLQISEEQQHPNEFIGQRAEQQLSTLADIGVKLSGTIENEVLAINFLLGEIEKVKAQARLDLYDIDVEVQYASGKFELWQMATSYRNVSNVVVKLAPKNVKSASYLLVNAHFDSEVHSPAAADDGVMIVIMLETLRVIAKSEKPLMHPIVFLFNGAEESNMLASHGFITQHPWAQYCKAVINLDSAGSGGREILFQSGPNHPWLMKYYKQSVPRPYATTIAEELFQNNFIPSDTDFRIFRDYGELPGLDMAHSLNGYVYHTKFDTFANLERHTCQTTGENVLALTWALANAPELKDPGDLAKGHTVFYDYLGWFMIFYTEDTGVILNITFSVCAIIAILVAVFLMSRSDDADSTKRVFLRFLTILVAQIITIAAAVGLAILVAVTIDGIGASECWYSEPWLVFGIYFCPVFFIMCLLPAIYMRWTKEGTNMHLDDSVACFMHSHCLILVCLCLVMTGLSIRSAFFPMIGIFFYTISVVLNIINHLWGKSYLHLLIHLLCQLLPFWFYTYQTFVFLTTFIPMQGRSGPTSKPEFMIAGICAIMAIHFGGFILPILHKFRKSKTFISLFGALFLIFIMIACLPVGFPInullKDVAVQRVYVLHTARTFYDERGFVYKNESGFYVQPVDKRINLLKKTVLQNAEPNSKIQEDCETELFCGLPLYNSRWMDWKNSSRWVVAPSPYLPIPTELKLTSKVYITGTRVRYGFSLKSADRVVIYIDPYANTKIVDWTFDKTPLKSNFSTPYFVYHIYSMDDRPLDFWIELDRGTPDYEGAALRLGIGAHFMYHDAYYTEEFKDFLKLFPEWSYPLDWLASYESRIF

Target ORF information:

RefSeq Version	XM_029549535.1
Organism	Bactrocera dorsalis(oriental fruit fly)
Definition	Bactrocera dorsalis endoplasmic reticulum metallopeptidase 1 (LOC105224581), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029549535.1

ORF Insert Sequence:

ATGAGCGTTA AGAAATTCGA AATACACAAC AACAACATAG AGCGAAAGAA AAGCAGAAAA 
TTTGATTGGT ACTGGGCACC CGTCTACTGT GCTGTCTGGT ATGTGCTTTT CTATGCCGCC 
GTCATACCGA GCTTCTATAA TTATCCAAAG ACGCTCCAAA TCAGCGAAGA ACAGCAGCAC 
CCCAACGAAT TTATAGGACA ACGTGCCGAA CAGCAACTAA GCACATTAGC TGATATTGGC 
GTTAAACTAT CGGGGACCAT AGAAAATGAA GTTCTAGCCA TCAATTTTCT ACTCGGCGAA 
ATTGAAAAAG TCAAAGCACA AGCGCGACTT GATCTCTACG ATATCGATGT GGAAGTGCAA 
TACGCTTCGG GAAAGTTCGA ATTGTGGCAA ATGGCTACCA GCTATCGCAA CGTATCCAAT 
GTGGTGGTGA AGTTGGCGCC AAAGAATGTT AAATCCGCTT CTTATTTACT GGTGAATGCG 
CATTTCGATT CAGAAGTGCA CTCGCCGGCT GCCGCAGATG ACGGTGTGAT GATCGTAATT 
ATGTTGGAGA CGCTCAGAGT GATTGCCAAA TCGGAAAAGC CGCTTATGCA TCCCATTGTA 
TTTCTCTTCA ATGGCGCTGA GGAGAGCAAT ATGCTGGCAT CGCACGGTTT CATCACACAG 
CACCCATGGG CGCAGTATTG CAAAGCCGTT ATCAATTTGG ACTCCGCTGG TTCGGGTGGT 
CGCGAAATTC TCTTTCAATC GGGTCCCAAT CACCCTTGGC TCATGAAGTA CTATAAACAA 
AGTGTACCCC GCCCCTATGC TACCACAATC GCTGAAGAGT TGTTCCAAAA TAATTTCATA 
CCATCGGATA CCGATTTTCG CATATTCCGT GACTATGGCG AGTTACCTGG CCTCGATATG 
GCGCATTCTC TCAACGGTTA TGTTTATCAC ACTAAATTCG ATACTTTTGC GAATCTCGAA 
AGGCACACGT GTCAGACGAC TGGTGAGAAT GTCTTAGCGT TAACGTGGGC ACTGGCCAAT 
GCACCGGAAT TGAAGGACCC AGGGGACCTA GCCAAAGGCC ACACTGTCTT CTACGATTAT 
CTCGGCTGGT TTATGATCTT CTACACAGAG GATACTGGTG TCATACTCAA TATCACCTTC 
TCTGTTTGTG CCATCATAGC CATCTTAGTT GCGGTTTTTC TGATGTCACG CTCTGACGAC 
GCCGACTCTA CCAAGCGTGT CTTCTTACGC TTTCTTACCA TATTGGTTGC ACAAATTATA 
ACAATAGCTG CGGCAGTGGG TCTAGCAATT TTAGTTGCGG TTACCATAGA TGGCATCGGT 
GCCTCAGAGT GTTGGTACTC AGAGCCATGG CTCGTCTTTG GCATCTACTT TTGCCCTGTC 
TTCTTTATTA TGTGTTTGCT GCCGGCCATT TACATGCGTT GGACCAAGGA AGGAACCAAT 
ATGCATCTTG ATGACTCTGT TGCCTGCTTC ATGCATTCGC ATTGCCTCAT TTTGGTGTGC 
CTTTGTCTGG TTATGACTGG TTTGAGTATA CGATCGGCTT TCTTTCCAAT GATTGGCATC 
TTCTTCTATA CCATATCGGT TGTATTGAAT ATAATAAATC ACTTGTGGGG CAAGAGCTAC 
TTGCACTTGC TTATACATCT TCTTTGTCAG TTGCTGCCAT TTTGGTTTTA TACCTATCAA 
ACATTTGTCT TTCTCACAAC ATTCATACCA ATGCAAGGAC GCAGTGGACC AACCAGTAAA 
CCGGAATTTA TGATTGCTGG CATTTGTGCT ATAATGGCTA TACATTTCGG TGGTTTCATT 
CTTCCAATAT TGCATAAGTT CCGCAAATCA AAAACTTTTA TAAGTTTATT TGGAGCTCTA 
TTTTTGATAT TCATTATGAT TGCTTGCCTG CCTGTTGGCT TCCCTATANA GAAGGATGTG 
GCTGTACAGA GAGTTTACGT TTTGCACACG GCACGCACAT TCTACGACGA GCGAGGTTTT 
GTCTACAAAA ACGAAAGCGG TTTCTATGTG CAACCTGTCG ATAAGCGTAT TAACTTATTG 
AAGAAAACCG TTTTACAGAA TGCTGAACCA AATTCGAAGA TTCAAGAAGA TTGTGAGACA 
GAACTTTTTT GCGGGCTGCC ATTATATAAT TCGCGTTGGA TGGATTGGAA AAACTCCTCC 
CGCTGGGTGG TGGCGCCAAG TCCATATCTG CCTATACCAA CTGAACTCAA GTTAACCTCA 
AAGGTTTATA TTACTGGGAC ACGAGTCCGT TATGGCTTTA GCTTAAAGTC CGCAGATCGC 
GTAGTGATCT ATATAGATCC TTATGCAAAT ACGAAAATTG TTGATTGGAC TTTTGATAAG 
ACGCCATTGA AAAGTAATTT CTCGACACCT TACTTTGTGT ATCACATCTA TTCGATGGAT 
GATAGGCCAT TGGATTTCTG GATTGAATTG GACCGCGGTA CTCCAGACTA TGAGGGCGCC 
GCTTTGCGTC TTGGCATCGG CGCACACTTT ATGTACCATG ATGCCTACTA TACTGAAGAA 
TTTAAAGACT TCCTCAAGCT ATTTCCCGAG TGGTCATATC CGTTAGACTG GTTAGCTTCC 
TATGAGAGTC GCATTTTCTA G