smc5 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	smc5
Entrez Gene ID	103042816
Full Name	structural maintenance of chromosomes 5
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007255019.2	XP_007255081.1	structural maintenance of chromosomes protein 5
XM_022687157.1	XP_022542878.1	structural maintenance of chromosomes protein 5

Pathways from BioSystems
Gene Ontology

The following smc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs37087	XM_007255019.2 Latest version!	Astyanax mexicanus structural maintenance of chromosomes 5 (smc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAs96798	XM_022687157.1 Latest version!	Astyanax mexicanus structural maintenance of chromosomes 5 (smc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smc5 ( XM_007255019.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007255081.1 Astyanax mexicanus structural maintenance of chromosomes 5 (smc5), mRNA.

Price & Availability↑

CloneID	OAs37087
Clone ID Related Accession (Same CDS sequence)	XM_007255019.2
Accession Version	XM_007255019.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-10
Organism	Astyanax mexicanus(Mexican tetra)
Product	structural maintenance of chromosomes protein 5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007255019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGTGG GCTATGAGGC GCTACAGCGG CGCGAAACGA AAGCGGAGAG GTGCGGGAGA 
ATGGCGCTCC AGAGCAAAAA GAGGAGAATA AGTCACATTT TAACAAACTC TCAGCTTCCC 
AACAGCCAGA ACCTGCTGCT GCCCTGCAGC TCGCAGAATG GCAGCTTCGC CCAGGAGAAC 
GGGCAGAGCG GCCCCTGCGT GGAGGGATCC ATTCGCCGCA TCACCATGCA AAACTTCCTC 
ACATATGATC ACTCAGAGGT TTACCCTGGA CCCAACTTAA ATATGATAGT GGGAGCCAAT 
GGCACTGGCA AGTCGAGTAT TGTCTGTGCC ATTTGTCTTG GACTGGCAGG GAAGACTGCT 
ATCCTGGGCA GAGGGGATAA GGTCAGCTTG TATGTTAAGA GAGGCTGTCA TAAAGGATCT 
GTGGAAATTG AACTGTACAG GAAAGAGGGA AACTTGGTGA TTAACAGGGA GATCCATGTA 
GAAAATAATC AGAGCGTGTG GATGATGAAC GGTAAACACT CCAGTCAGAA GGCTGTGGAA 
GAAGCAGTCA AGGAGCTCCA GATTCAGGTC GGAAACCTCT GTCAGTTTCT GCCTCAGGAG 
AAAGTTGGTG AATTCGCTAA AATGAGTAAG ATTGAGCTTC TTGAAGCCAC CGAAAAATCT 
GTTGGACCAC CAGAGATGTA CAACTTCCAC TGTGAGCTGA AGACCTGTCG CACCAAAGAG 
AGGGACCTGG AGAATTTGTG TAAAGAGAAG GCCAACTTTC TAGAGAAAGC AAAGCAGAGG 
AACGAGCGCA ATAAGGTGGA TGTGGAACGG TACCACATGA AGAGGAGACA TCTGGATATG 
ATCCAGATGT TGGAGAAAAA GAAGCCTTGG GTGGAGTACG AGACAGCTCG TAAAGAACTG 
GAAGAAGTTA AAAAGCAGAG GGAAGAAATG AAAAAGACGG CAAAGTCACT AAAGGAAACT 
CAAGCTCCCC TTCTGAAAAA GATCCAGGCT ATGGAAAGCA AGCTACAGCC TATTGAGAAT 
CAGATGAAGG AGAAGACTGT TAAAATCAGA GAGGCGTCAC AGAGATGCAA ACACAAGCAA 
GACCAGCTGG ACCGGAAACA TAAGGAGATT GATGACATCA AGCAGGCCCT TAGTCTGAAG 
CAGACTGAGG AAACTGATCG GCAGAAGCGA ATCGGGAACA CGCAACGAAT GATCCAAGAC 
CTGCAGAGGG AGCTGGATAA CATGGGCAGC CAAGAGGATG TCACACCAAA GATTGAAAAA 
GTCAACGCTG ATCTCAGGAC CATCTTGGAG GAGAAGGCCA GACTGGATGG AGAGCAGCAG 
GATCTGCGCA GGGATAAAGA CGAGGGCTTC GGGGAGCTGA GACGTCTACA GATTCGTCTG 
AGGGGTCTAG AGGACATGAT GAACAGAAAG GAGGAAATGT TGCGCAACCG CTTCCGGGAC 
ACCTACCAGG CCTTACAGTG GTTGAGGAAG AACCGCAATC TTTTCGAGGG AAATGTCTAC 
GAGCCCATGA TGCTGGTGAT CAATGTACGA GATCCAAAAT TTGCAAAATA CATTGAGAGC 
CACGTTCCCG TCAATGACCT GAGAGCCTTC GTCTTCCAGA AGCAGCGGGA TATGGAGAAG 
TTCATGTCAG AGGTTAGAGA TAATCAAAAG CTGAGAGTTA ACAGCGTTAT TGCACCTTTG 
GAATCCTGTG CTAATCGGCC ACCATCACGA CCCCTCGAAT CTCTCAAGCG CTTTGGATTC 
ATCTCCTACC TCCGAGAGCT GTTTGATGCA CCTGATGAGG TCATGAGTTA CCTGGTTCAT 
CAGTACAGAG TCAATGATGT TCCTGTGGGC ACTGAAAAGA CCAAGAGCAT GATCGAGACG 
GTGATAAAAG AATCTCAGCT GAGGATGATC TACACTGCAG AGGAGAAATA CACAGTGAAG 
AAATCCGCCT ACTCCAACAA GACCATCTCC AGTAACTCCG CCCTGCGCCC CTCTCAGTTC 
CTCAGCATGG CCATCGACTC TGAGGAGAAA CGGCAGCTAG AAGAACAGCT GAGCGCGGCT 
CAGCAGAAGG TGGAGGACGT TGATGCTCGG ATGAAATCCA TTCAGGAGCA GCAGGGAAAA 
CTGGACCGGC GGGACAACGA ACTCCGCACT GAGAAGAAGC GTCTCTCTGA GATAAAAGGC 
AAGAAGAGAC AACTGGAGCA GAAAATCAGC ACAAAACAGG ACAGCCTGAG GCAGATGGAG 
CAGAGCGGGA TCAACCTTCA GGAAGCTGAG GAGGAGGCTA AGGCCAAAAT CTCTGACGTA 
AATGACCAGA AGGTGGCCAT TGTGGAGGAG ATCTTGAATT TCATGAAGCT GAGAGCCAAG 
CTGAGCATGG AGAAAGTTTA CCTGGCTCTG GAGTCAGCAG GTCTGGCGGC TGAGAAAACT 
CAACTGGAGT CAGATCTCAG GGAGGGGTCG GCAGAACTCA GGAGAGCCGA GCAAGAGTTT 
GCTCAGCTGG ACCAGAGGAA GACGAGTCTG TTGGGGACGT GTAAGAGCTT AATGAGGAGA 
GCCAGTGAGA TTTGCAACAT GAACCCAGGA GAAACTGTAG TACCAGAGAT TCTGCGCACG 
GCTTTCAGCG AGCTGCCGGA CACTCTGGAT GAGATTGACG CCATGTTAAA CGAAGAGCGA 
GCCAGAGCAG AGTGCTTCAC TGGCCTTAAT GAAGCAGTGG TGGAGGAATA CAGTAAGAGA 
GAGAAGGAAA TAAAGAACAT GGAGAAGGAG CTGGAGGAAA AGACGAAGGC TCTGAACGAA 
TACAGACAAA ACATCTCAGA GGCTAAGGAA CGCTGGCTGA ATCCCTTGAA GCTGTTGGTG 
GAGCAGATTA ATGAAAAGTT CAGTGATTTC TTCCGCTCCA TGAACTGCGC TGGGGAGGTG 
GACCTGCACT CTGAAAATGA GGTGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007255081.1
CDS	18..2927
Translation	MTVGYEALQRRETKAERCGRMALQSKKRRISHILTNSQLPNSQNLLLPCSSQNGSFAQENGQSGPCVEGSIRRITMQNFLTYDHSEVYPGPNLNMIVGANGTGKSSIVCAICLGLAGKTAILGRGDKVSLYVKRGCHKGSVEIELYRKEGNLVINREIHVENNQSVWMMNGKHSSQKAVEEAVKELQIQVGNLCQFLPQEKVGEFAKMSKIELLEATEKSVGPPEMYNFHCELKTCRTKERDLENLCKEKANFLEKAKQRNERNKVDVERYHMKRRHLDMIQMLEKKKPWVEYETARKELEEVKKQREEMKKTAKSLKETQAPLLKKIQAMESKLQPIENQMKEKTVKIREASQRCKHKQDQLDRKHKEIDDIKQALSLKQTEETDRQKRIGNTQRMIQDLQRELDNMGSQEDVTPKIEKVNADLRTILEEKARLDGEQQDLRRDKDEGFGELRRLQIRLRGLEDMMNRKEEMLRNRFRDTYQALQWLRKNRNLFEGNVYEPMMLVINVRDPKFAKYIESHVPVNDLRAFVFQKQRDMEKFMSEVRDNQKLRVNSVIAPLESCANRPPSRPLESLKRFGFISYLRELFDAPDEVMSYLVHQYRVNDVPVGTEKTKSMIETVIKESQLRMIYTAEEKYTVKKSAYSNKTISSNSALRPSQFLSMAIDSEEKRQLEEQLSAAQQKVEDVDARMKSIQEQQGKLDRRDNELRTEKKRLSEIKGKKRQLEQKISTKQDSLRQMEQSGINLQEAEEEAKAKISDVNDQKVAIVEEILNFMKLRAKLSMEKVYLALESAGLAAEKTQLESDLREGSAELRRAEQEFAQLDQRKTSLLGTCKSLMRRASEICNMNPGETVVPEILRTAFSELPDTLDEIDAMLNEERARAECFTGLNEAVVEEYSKREKEIKNMEKELEEKTKALNEYRQNISEAKERWLNPLKLLVEQINEKFSDFFRSMNCAGEVDLHSENEVS

Target ORF information:

RefSeq Version	XM_007255019.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus structural maintenance of chromosomes 5 (smc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007255019.2

ORF Insert Sequence:

ATGACAGTGG GCTATGAGGC GCTACAGCGG CGCGAAACGA AAGCGGAGAG GTGCGGGAGA 
ATGGCGCTCC AGAGCAAAAA GAGGAGAATA AGTCACATTT TAACAAACTC TCAGCTTCCC 
AACAGCCAGA ACCTGCTGCT GCCCTGCAGC TCGCAGAATG GCAGCTTCGC CCAGGAGAAC 
GGGCAGAGCG GCCCCTGCGT GGAGGGATCC ATTCGCCGCA TCACCATGCA AAACTTCCTC 
ACATATGATC ACTCAGAGGT TTACCCTGGA CCCAACTTAA ATATGATAGT GGGAGCCAAT 
GGCACTGGCA AGTCGAGTAT TGTCTGTGCC ATTTGTCTTG GACTGGCAGG GAAGACTGCT 
ATCCTGGGCA GAGGGGATAA GGTCAGCTTG TATGTTAAGA GAGGCTGTCA TAAAGGATCT 
GTGGAAATTG AACTGTACAG GAAAGAGGGA AACTTGGTGA TTAACAGGGA GATCCATGTA 
GAAAATAATC AGAGCGTGTG GATGATGAAC GGTAAACACT CCAGTCAGAA GGCTGTGGAA 
GAAGCAGTCA AGGAGCTCCA GATTCAGGTC GGAAACCTCT GTCAGTTTCT GCCTCAGGAG 
AAAGTTGGTG AATTCGCTAA AATGAGTAAG ATTGAGCTTC TTGAAGCCAC CGAAAAATCT 
GTTGGACCAC CAGAGATGTA CAACTTCCAC TGTGAGCTGA AGACCTGTCG CACCAAAGAG 
AGGGACCTGG AGAATTTGTG TAAAGAGAAG GCCAACTTTC TAGAGAAAGC AAAGCAGAGG 
AACGAGCGCA ATAAGGTGGA TGTGGAACGG TACCACATGA AGAGGAGACA TCTGGATATG 
ATCCAGATGT TGGAGAAAAA GAAGCCTTGG GTGGAGTACG AGACAGCTCG TAAAGAACTG 
GAAGAAGTTA AAAAGCAGAG GGAAGAAATG AAAAAGACGG CAAAGTCACT AAAGGAAACT 
CAAGCTCCCC TTCTGAAAAA GATCCAGGCT ATGGAAAGCA AGCTACAGCC TATTGAGAAT 
CAGATGAAGG AGAAGACTGT TAAAATCAGA GAGGCGTCAC AGAGATGCAA ACACAAGCAA 
GACCAGCTGG ACCGGAAACA TAAGGAGATT GATGACATCA AGCAGGCCCT TAGTCTGAAG 
CAGACTGAGG AAACTGATCG GCAGAAGCGA ATCGGGAACA CGCAACGAAT GATCCAAGAC 
CTGCAGAGGG AGCTGGATAA CATGGGCAGC CAAGAGGATG TCACACCAAA GATTGAAAAA 
GTCAACGCTG ATCTCAGGAC CATCTTGGAG GAGAAGGCCA GACTGGATGG AGAGCAGCAG 
GATCTGCGCA GGGATAAAGA CGAGGGCTTC GGGGAGCTGA GACGTCTACA GATTCGTCTG 
AGGGGTCTAG AGGACATGAT GAACAGAAAG GAGGAAATGT TGCGCAACCG CTTCCGGGAC 
ACCTACCAGG CCTTACAGTG GTTGAGGAAG AACCGCAATC TTTTCGAGGG AAATGTCTAC 
GAGCCCATGA TGCTGGTGAT CAATGTACGA GATCCAAAAT TTGCAAAATA CATTGAGAGC 
CACGTTCCCG TCAATGACCT GAGAGCCTTC GTCTTCCAGA AGCAGCGGGA TATGGAGAAG 
TTCATGTCAG AGGTTAGAGA TAATCAAAAG CTGAGAGTTA ACAGCGTTAT TGCACCTTTG 
GAATCCTGTG CTAATCGGCC ACCATCACGA CCCCTCGAAT CTCTCAAGCG CTTTGGATTC 
ATCTCCTACC TCCGAGAGCT GTTTGATGCA CCTGATGAGG TCATGAGTTA CCTGGTTCAT 
CAGTACAGAG TCAATGATGT TCCTGTGGGC ACTGAAAAGA CCAAGAGCAT GATCGAGACG 
GTGATAAAAG AATCTCAGCT GAGGATGATC TACACTGCAG AGGAGAAATA CACAGTGAAG 
AAATCCGCCT ACTCCAACAA GACCATCTCC AGTAACTCCG CCCTGCGCCC CTCTCAGTTC 
CTCAGCATGG CCATCGACTC TGAGGAGAAA CGGCAGCTAG AAGAACAGCT GAGCGCGGCT 
CAGCAGAAGG TGGAGGACGT TGATGCTCGG ATGAAATCCA TTCAGGAGCA GCAGGGAAAA 
CTGGACCGGC GGGACAACGA ACTCCGCACT GAGAAGAAGC GTCTCTCTGA GATAAAAGGC 
AAGAAGAGAC AACTGGAGCA GAAAATCAGC ACAAAACAGG ACAGCCTGAG GCAGATGGAG 
CAGAGCGGGA TCAACCTTCA GGAAGCTGAG GAGGAGGCTA AGGCCAAAAT CTCTGACGTA 
AATGACCAGA AGGTGGCCAT TGTGGAGGAG ATCTTGAATT TCATGAAGCT GAGAGCCAAG 
CTGAGCATGG AGAAAGTTTA CCTGGCTCTG GAGTCAGCAG GTCTGGCGGC TGAGAAAACT 
CAACTGGAGT CAGATCTCAG GGAGGGGTCG GCAGAACTCA GGAGAGCCGA GCAAGAGTTT 
GCTCAGCTGG ACCAGAGGAA GACGAGTCTG TTGGGGACGT GTAAGAGCTT AATGAGGAGA 
GCCAGTGAGA TTTGCAACAT GAACCCAGGA GAAACTGTAG TACCAGAGAT TCTGCGCACG 
GCTTTCAGCG AGCTGCCGGA CACTCTGGAT GAGATTGACG CCATGTTAAA CGAAGAGCGA 
GCCAGAGCAG AGTGCTTCAC TGGCCTTAAT GAAGCAGTGG TGGAGGAATA CAGTAAGAGA 
GAGAAGGAAA TAAAGAACAT GGAGAAGGAG CTGGAGGAAA AGACGAAGGC TCTGAACGAA 
TACAGACAAA ACATCTCAGA GGCTAAGGAA CGCTGGCTGA ATCCCTTGAA GCTGTTGGTG 
GAGCAGATTA ATGAAAAGTT CAGTGATTTC TTCCGCTCCA TGAACTGCGC TGGGGAGGTG 
GACCTGCACT CTGAAAATGA GGTGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smc5 ( XM_022687157.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022542878.1 Astyanax mexicanus structural maintenance of chromosomes 5 (smc5), mRNA.

Price & Availability↑

CloneID	OAs96798
Clone ID Related Accession (Same CDS sequence)	XM_022687157.1
Accession Version	XM_022687157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Astyanax mexicanus(Mexican tetra)
Product	structural maintenance of chromosomes protein 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019171430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTCC AGAGCAAAAA GAGGAGAATA AGTCACATTT TAACAAACTC TCTGCTTCCC 
AACAGCCAGA ACCTGCTGCT GCCCTGCAGC TCGCAGAATG GCAGCTTCGC CCAGGAGAAC 
GGGCAGAGCG GCCCCTGCGT GGAGGGATCC ATTCGCCGCA TCACCATGCA AAACTTCCTC 
ACATATGATC ACTCAGAGGT TTACCCTGGA CCCAACTTAA ATATGATAGT GGGAGCCAAT 
GGCACTGGCA AGTCGAGTAT TGTCTGTGCC ATTTGTCTTG GACTGGCAGG GAAGACTGCT 
ATCCTGGGCA GAGGGGATAA GGTCAGCTTG TATGTTAAGA GAGGCTGTCA TAAAGGATCT 
GTGGAAATTG AACTGTACAG GAAAGAGGGA AACTTGGTGA TTAACAGGGA GATCCATGTA 
GAAAATAATC AGAGCGTGTG GATGATGAAC GGTAAACACT CCAGTCAGAA GGCTGTGGAA 
GAAGCAGTCA AGGAGCTCCA GATTCAGGTC GGAAACCTCT GTCAGTTTCT GCCTCAGGAG 
AAAGTTGGTG AATTCGCTAA AATGAGTAAG ATTGAGCTTC TTGAAGCCAC CGAAAAATCT 
GTTGGACCAC CAGAGATGTA CAACTTCCAC TGTGAGCTGA AGACCTGTCG CACCAAAGAG 
AGGGACCTGG AGAATTTGTG TAAAGAGAAG GCCAACTTTC TAGAGAAAGC AAAGCAGAGG 
AACGAGCGCA ATAAGGTGGA TGTGGAACGG TACCACATGA AGAGGAGACA TCTGGATATG 
ATCCAGATGT TGGAGAAAAA GAAGCCTTGG GTGGAGTACG AGACAGCTCG TAAAGAACTG 
GAAGAAGTTA AAAAGCAGAG GGAAGAAATG AAAAAGACGG CAAAGTCACT AAAGGAAACT 
CAAGCTCCCC TTCTGAAAAA GATCCAGGCT ATGGAAAGCA AGCTACAGCC TATTGAGAAT 
CAGATGAAGG AGAAGACTGT TAAAATCAGA GAGGCGTCAC AGAGATGCAA ACACAAGCAA 
GACCAGCTGG ACCGGAAACA TAAGGAGATT GATGACATCA AGCAGGCCCT TAGTCTGAAG 
CAGACTGAGG AAACTGATCG GCAGAAGCGA ATCGGGAACA CGCAACGAAT GATCCAAGAC 
CTGCAGAGGG AGCTGGATAA CATGGGCAGC CAAGAGGATG TCACACCAAA GATTGAAAAA 
GTCAACGCTG ATCTCAGGAC CATCTTGGAG GAGAAGGCCA GACTGGATGG AGAGCAGCAG 
GATCTGCGCA GGGATAAAGA CGAGGGCTTC GGGGAGCTGA GACGTCTACA GATTCGTCTG 
AGGGGTCTAG AGGACATGAT GAACAGAAAG GAGGAAATGT TGCGCAACCG CTTCCGGGAC 
ACCTACCAGG CCTTACAGTG GTTGAGGAAG AACCGCAATC TTTTCGAGGG AAATGTTTAC 
GAGCCCATGA TGCTGGTGAT CAATGTACGA GATCCAAAAT TTGCAAAATA CATTGAGAGC 
CACGTTCCCG TCAATGACCT GAGAGCCTTC GTCTTCCAGA AGCAGCGGGA TATGGAGAAG 
TTCATGTCAG AGGTTAGAGA TAATCAAAAG CTGAGAGTTA ACAGCGTTAT TGCACCTTTG 
GAATCCTGTG CTAATCGGCC ACCATCACGA CCCCTCGAAT CTCTCAAGCG CTTTGGGTTC 
ATCTCCTACC TCCGAGAGCT GTTTGATGCA CCTGATGAGG TCATGAGTTA CCTGGTTCAT 
CAGTACAGAG TCAATGATGT TCCTGTGGGC ACTGAAAAGA CCAAGAGCAT GATCGAGACG 
GTGATAAAAG AATCTCAGCT GAGGATGATC TACACTGCAG AGGAGAAATA CACAGTGAAG 
AAATCCGCCT ACTCCAACAA GACCATCTCC AGTAACTCCG CCCTGCGCCC CTCTCAGTTC 
CTCAGCATGG CCATCGACTC TGAGGAGAAA CGGCAGCTAG AAGAACAGCT GAGCGCGGCT 
CAGCAGAAGG TGGAGGACGT TGATGCTCGG ATGAAATCCA TTCAGGAGCA GCAGGGAAAA 
CTGGACCGGC GGGACAACGA ACTCCGCACT GAGAAGAAGC GTCTCTCGGA GATAAAAGGC 
AAGAAGAGAC AACTGGAGCA GAAAATCAGC ACAAAACAGG ACAGCCTGAG GCAGATGGAG 
CAGAGCGGGA TCAACCTTCA GGAAGCTGAG GAGGAGGCTA AGGCCAAAAT CTCTGACGTA 
AATGACCAGA AGGTGGCCAT TGTGGAGGAG ATCTTGAATT TCATGAAGCT GAGAGCCAAG 
CTGAGCATGG AGAAAGTTTA CCTGGCTCTG GAGTCAGCAG GTCTGGCGGC TGAGAAAACT 
CAACTGGAGT CAGATCTCAG GGAGGGGTCG GCAGAACTCA GGAGAGCCGA GCAAGAGTTT 
GCTCAGCTGG ACCAGAGGAA GACGAGTCTG TTGGGGACGT GTAAGAGCTT AATGAGGAGA 
GCCAGTGAGA TTTGCAACAT GAACCCAGGA GAAACTGTAG TACCAGAGAT TCTGCGCACG 
GCTTTCAGCG AGCTGCCGGA CACTCTGGAT GAGATTGACG CCATGTTAAA CGAAGAGCGA 
GCCAGAGCAG AGTGCTTCAC TGGCCTTAAT GAAGCAGTGG TGGAGGAATA CAGTAAGAGA 
GAGAAGGAAA TAAAGAACAT GGAGAAGGAG CTGGAGGAAA AGACGAAGGC TCTGAACGAA 
TACAGACAAA ACATCTCAGA GGCTAAGGAA CGCTGGCTGA ATCCCTTGAA GCTGTTGGTG 
GAGCAGATTA ATGAAAAGTT CAGTGATTTC TTCCGCTCCA TGAACTGCGC TGGGGAGGTG 
GACCTGCACT CTGAAAATGA GGAGGAGTAT GATAAGTACG GGATCCGGAT CCGGGTGAAG 
TTCCGCAGCA ACACTCAGCT ACACGAGCTG ACCCCGCACC ATCAGAGCGG CGGAGAGCGC 
AGCGTCTCCA CCATGCTCTA CCTCATGGCC CTGCAGGAGC TCAACCGCTG CCCCTTCAGA 
GTCGTCGATG AGATCAACCA GGGTATGGAT CCGGTGAATG AGAGGAGGGT ATTTGATATC 
GTGGTGAAGA CGGCCTGTGG TGGAAACACA TCTCAGTATT TCTTCATCAC ACCTAAGCTT 
CTACAGAATC TGACGTATGC AGAGGAGATG ACCGTCCTGT GCGTACACAA CGGCCCCCAC 
ATGCTCCCCC CAAACAAGTG GAACGACAGG GCTTTTCTCA GGCGAGTCAA ACGGAGGACC 
AACCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022542878.1
CDS	59..3307
Translation	MALQSKKRRISHILTNSLLPNSQNLLLPCSSQNGSFAQENGQSGPCVEGSIRRITMQNFLTYDHSEVYPGPNLNMIVGANGTGKSSIVCAICLGLAGKTAILGRGDKVSLYVKRGCHKGSVEIELYRKEGNLVINREIHVENNQSVWMMNGKHSSQKAVEEAVKELQIQVGNLCQFLPQEKVGEFAKMSKIELLEATEKSVGPPEMYNFHCELKTCRTKERDLENLCKEKANFLEKAKQRNERNKVDVERYHMKRRHLDMIQMLEKKKPWVEYETARKELEEVKKQREEMKKTAKSLKETQAPLLKKIQAMESKLQPIENQMKEKTVKIREASQRCKHKQDQLDRKHKEIDDIKQALSLKQTEETDRQKRIGNTQRMIQDLQRELDNMGSQEDVTPKIEKVNADLRTILEEKARLDGEQQDLRRDKDEGFGELRRLQIRLRGLEDMMNRKEEMLRNRFRDTYQALQWLRKNRNLFEGNVYEPMMLVINVRDPKFAKYIESHVPVNDLRAFVFQKQRDMEKFMSEVRDNQKLRVNSVIAPLESCANRPPSRPLESLKRFGFISYLRELFDAPDEVMSYLVHQYRVNDVPVGTEKTKSMIETVIKESQLRMIYTAEEKYTVKKSAYSNKTISSNSALRPSQFLSMAIDSEEKRQLEEQLSAAQQKVEDVDARMKSIQEQQGKLDRRDNELRTEKKRLSEIKGKKRQLEQKISTKQDSLRQMEQSGINLQEAEEEAKAKISDVNDQKVAIVEEILNFMKLRAKLSMEKVYLALESAGLAAEKTQLESDLREGSAELRRAEQEFAQLDQRKTSLLGTCKSLMRRASEICNMNPGETVVPEILRTAFSELPDTLDEIDAMLNEERARAECFTGLNEAVVEEYSKREKEIKNMEKELEEKTKALNEYRQNISEAKERWLNPLKLLVEQINEKFSDFFRSMNCAGEVDLHSENEEEYDKYGIRIRVKFRSNTQLHELTPHHQSGGERSVSTMLYLMALQELNRCPFRVVDEINQGMDPVNERRVFDIVVKTACGGNTSQYFFITPKLLQNLTYAEEMTVLCVHNGPHMLPPNKWNDRAFLRRVKRRTNQ

Target ORF information:

RefSeq Version	XM_022687157.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus structural maintenance of chromosomes 5 (smc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022687157.1

ORF Insert Sequence:

ATGGCGCTCC AGAGCAAAAA GAGGAGAATA AGTCACATTT TAACAAACTC TCTGCTTCCC 
AACAGCCAGA ACCTGCTGCT GCCCTGCAGC TCGCAGAATG GCAGCTTCGC CCAGGAGAAC 
GGGCAGAGCG GCCCCTGCGT GGAGGGATCC ATTCGCCGCA TCACCATGCA AAACTTCCTC 
ACATATGATC ACTCAGAGGT TTACCCTGGA CCCAACTTAA ATATGATAGT GGGAGCCAAT 
GGCACTGGCA AGTCGAGTAT TGTCTGTGCC ATTTGTCTTG GACTGGCAGG GAAGACTGCT 
ATCCTGGGCA GAGGGGATAA GGTCAGCTTG TATGTTAAGA GAGGCTGTCA TAAAGGATCT 
GTGGAAATTG AACTGTACAG GAAAGAGGGA AACTTGGTGA TTAACAGGGA GATCCATGTA 
GAAAATAATC AGAGCGTGTG GATGATGAAC GGTAAACACT CCAGTCAGAA GGCTGTGGAA 
GAAGCAGTCA AGGAGCTCCA GATTCAGGTC GGAAACCTCT GTCAGTTTCT GCCTCAGGAG 
AAAGTTGGTG AATTCGCTAA AATGAGTAAG ATTGAGCTTC TTGAAGCCAC CGAAAAATCT 
GTTGGACCAC CAGAGATGTA CAACTTCCAC TGTGAGCTGA AGACCTGTCG CACCAAAGAG 
AGGGACCTGG AGAATTTGTG TAAAGAGAAG GCCAACTTTC TAGAGAAAGC AAAGCAGAGG 
AACGAGCGCA ATAAGGTGGA TGTGGAACGG TACCACATGA AGAGGAGACA TCTGGATATG 
ATCCAGATGT TGGAGAAAAA GAAGCCTTGG GTGGAGTACG AGACAGCTCG TAAAGAACTG 
GAAGAAGTTA AAAAGCAGAG GGAAGAAATG AAAAAGACGG CAAAGTCACT AAAGGAAACT 
CAAGCTCCCC TTCTGAAAAA GATCCAGGCT ATGGAAAGCA AGCTACAGCC TATTGAGAAT 
CAGATGAAGG AGAAGACTGT TAAAATCAGA GAGGCGTCAC AGAGATGCAA ACACAAGCAA 
GACCAGCTGG ACCGGAAACA TAAGGAGATT GATGACATCA AGCAGGCCCT TAGTCTGAAG 
CAGACTGAGG AAACTGATCG GCAGAAGCGA ATCGGGAACA CGCAACGAAT GATCCAAGAC 
CTGCAGAGGG AGCTGGATAA CATGGGCAGC CAAGAGGATG TCACACCAAA GATTGAAAAA 
GTCAACGCTG ATCTCAGGAC CATCTTGGAG GAGAAGGCCA GACTGGATGG AGAGCAGCAG 
GATCTGCGCA GGGATAAAGA CGAGGGCTTC GGGGAGCTGA GACGTCTACA GATTCGTCTG 
AGGGGTCTAG AGGACATGAT GAACAGAAAG GAGGAAATGT TGCGCAACCG CTTCCGGGAC 
ACCTACCAGG CCTTACAGTG GTTGAGGAAG AACCGCAATC TTTTCGAGGG AAATGTTTAC 
GAGCCCATGA TGCTGGTGAT CAATGTACGA GATCCAAAAT TTGCAAAATA CATTGAGAGC 
CACGTTCCCG TCAATGACCT GAGAGCCTTC GTCTTCCAGA AGCAGCGGGA TATGGAGAAG 
TTCATGTCAG AGGTTAGAGA TAATCAAAAG CTGAGAGTTA ACAGCGTTAT TGCACCTTTG 
GAATCCTGTG CTAATCGGCC ACCATCACGA CCCCTCGAAT CTCTCAAGCG CTTTGGGTTC 
ATCTCCTACC TCCGAGAGCT GTTTGATGCA CCTGATGAGG TCATGAGTTA CCTGGTTCAT 
CAGTACAGAG TCAATGATGT TCCTGTGGGC ACTGAAAAGA CCAAGAGCAT GATCGAGACG 
GTGATAAAAG AATCTCAGCT GAGGATGATC TACACTGCAG AGGAGAAATA CACAGTGAAG 
AAATCCGCCT ACTCCAACAA GACCATCTCC AGTAACTCCG CCCTGCGCCC CTCTCAGTTC 
CTCAGCATGG CCATCGACTC TGAGGAGAAA CGGCAGCTAG AAGAACAGCT GAGCGCGGCT 
CAGCAGAAGG TGGAGGACGT TGATGCTCGG ATGAAATCCA TTCAGGAGCA GCAGGGAAAA 
CTGGACCGGC GGGACAACGA ACTCCGCACT GAGAAGAAGC GTCTCTCGGA GATAAAAGGC 
AAGAAGAGAC AACTGGAGCA GAAAATCAGC ACAAAACAGG ACAGCCTGAG GCAGATGGAG 
CAGAGCGGGA TCAACCTTCA GGAAGCTGAG GAGGAGGCTA AGGCCAAAAT CTCTGACGTA 
AATGACCAGA AGGTGGCCAT TGTGGAGGAG ATCTTGAATT TCATGAAGCT GAGAGCCAAG 
CTGAGCATGG AGAAAGTTTA CCTGGCTCTG GAGTCAGCAG GTCTGGCGGC TGAGAAAACT 
CAACTGGAGT CAGATCTCAG GGAGGGGTCG GCAGAACTCA GGAGAGCCGA GCAAGAGTTT 
GCTCAGCTGG ACCAGAGGAA GACGAGTCTG TTGGGGACGT GTAAGAGCTT AATGAGGAGA 
GCCAGTGAGA TTTGCAACAT GAACCCAGGA GAAACTGTAG TACCAGAGAT TCTGCGCACG 
GCTTTCAGCG AGCTGCCGGA CACTCTGGAT GAGATTGACG CCATGTTAAA CGAAGAGCGA 
GCCAGAGCAG AGTGCTTCAC TGGCCTTAAT GAAGCAGTGG TGGAGGAATA CAGTAAGAGA 
GAGAAGGAAA TAAAGAACAT GGAGAAGGAG CTGGAGGAAA AGACGAAGGC TCTGAACGAA 
TACAGACAAA ACATCTCAGA GGCTAAGGAA CGCTGGCTGA ATCCCTTGAA GCTGTTGGTG 
GAGCAGATTA ATGAAAAGTT CAGTGATTTC TTCCGCTCCA TGAACTGCGC TGGGGAGGTG 
GACCTGCACT CTGAAAATGA GGAGGAGTAT GATAAGTACG GGATCCGGAT CCGGGTGAAG 
TTCCGCAGCA ACACTCAGCT ACACGAGCTG ACCCCGCACC ATCAGAGCGG CGGAGAGCGC 
AGCGTCTCCA CCATGCTCTA CCTCATGGCC CTGCAGGAGC TCAACCGCTG CCCCTTCAGA 
GTCGTCGATG AGATCAACCA GGGTATGGAT CCGGTGAATG AGAGGAGGGT ATTTGATATC 
GTGGTGAAGA CGGCCTGTGG TGGAAACACA TCTCAGTATT TCTTCATCAC ACCTAAGCTT 
CTACAGAATC TGACGTATGC AGAGGAGATG ACCGTCCTGT GCGTACACAA CGGCCCCCAC 
ATGCTCCCCC CAAACAAGTG GAACGACAGG GCTTTTCTCA GGCGAGTCAA ACGGAGGACC 
AACCAATAA