ube3a cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube3a
Entrez Gene ID	103042789
Full Name	ubiquitin protein ligase E3A
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007249113.1	XP_007249175.1	ubiquitin-protein ligase E3A
XM_007249113.2	XP_007249175.2	ubiquitin-protein ligase E3A

Pathways from BioSystems
Gene Ontology

The following ube3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs107892	XM_007249113.2 Latest version!	Astyanax mexicanus ubiquitin protein ligase E3A (ube3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAs31664	XM_007249113.1 Latest version!	Astyanax mexicanus ubiquitin protein ligase E3A (ube3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube3a ( XM_007249113.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007249175.2 Astyanax mexicanus ubiquitin protein ligase E3A (ube3a), mRNA.

Price & Availability↑

CloneID	OAs107892
Clone ID Related Accession (Same CDS sequence)	XM_007249113.2
Accession Version	XM_007249113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Astyanax mexicanus(Mexican tetra)
Product	ubiquitin-protein ligase E3A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035909.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007249113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAG CGGCTGCAAA GCATCTAATT GAGCGTTACT ACCACCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAACGAGGCT TGTGGCTCCT CTCCGGGTTT CCGGCGCATG 
GATAACAATG CAGCGGCCAT CAAGGCCCTG GAACTCTATA AGAATAACGC CAAACTCTGT 
GATCCTCACC CCTCCAAGAA AGGTGCCAGC TCGGCTTTCC CGGAGAACAG TGCCAAAGGA 
GCTCACAACT TTTCTGCTTG CAGCAACGGG AAGATGAACC ACAAGGATCT GCCTCCCTCA 
AGGGAAGACT TTAGAGATTT GAACTATTTG ACGGAGGAGA AGGTTTATGA AATTCTGGCT 
CTCTGTGGTG AGACAGAAGA CTACTCCCCT CTAATCCGTG TCATCGGGAG GGTCTTCTCT 
AGTGCTGAAA GCTTAGTGCA GAGCTTCCGG AAGGCTAAAC AACACACCAA GGAGGAGCTG 
AAATCGCTGC AGGCCAAGGA CGAGGACAAG GATGAAGATG AGAAAGAAAA CGCCTCTGGT 
TCGGCCAGTG CCATGGAAGT GGATCCTGAT GCTGCTGCCG CTTCCAGTGG AGAGGGGGCC 
TCTCATGATG GTAATAATGT GCAAAAGCTG GGCCCGGTTG AAGTGTCTGT GGACATTGAC 
GCAGTAAGAA GAGTTTATGA CAGACTATTG TCAAACGAGA AGATTGAGGC GGCATTCCTC 
AACGCATTAG TGTATCTGTC GCCCAATGTG GAATGCGACC TCACGTATCA CAACGTGTAT 
GCCGCAGACC CCAATTATCT AAACGTGTTC ATCATTGTCA TGGAGAACAG CAACCTGCAC 
AGCCCTGAGT ACCTAGAGAT TGCCCTGCCC CAGTTTTGCA AGGCCATGAG CAAGCTACCT 
CTCCCAGCTC TGGCCAAGCT AGCACGTCTT TGGTCGCAGT ACGGAGCAGA CCAGATAAGG 
CGCCTGGTGG AGACCTTCCA GCAGCTCATC ACCTACACAG TTATAAGCAA CGAGTTCAAC 
AGTGACAACC TGGTAAATGA GGATGACGGC GTGGTGGCGG CCACCAAGTG CTTGAAAATC 
GTCTACTATG CAAACGTGCT GGGCGGTGAC CTGGACACAG ACCACAACGA GGAGGAGGAT 
GACGAGCCCA TCCCTGAGTC CAGTGAGCTG ACCCTACAGG AGCTGCTGGG GGAGGAGCGA 
CGCAACAAGA AAGGGCCGCG TGTGGACCCG CTTGAGACTG AGCTGGGCAT TCGCACCTCG 
GACTGCCGCA AGCCCCTCAT TCCCTTCGAG GAGTTCATCA ATGAGCCGCT AAACGATGTG 
CTGGAAATGG ACAAGGACTA CACCTTCTTC AAGGTGGAGA CGGAGAGCAA GTTCTCCTTC 
ATGACCTGTC CATTCATCCT TAACGCTGTG ACAAAAAACC TGGGCCTCTA CTATGACAAC 
CGGATCCGCA TGTACAGTGA GCGCCGAATC ACTGCTCTCT ACAGCCTGGT GCAAGGACAG 
CAGCTCAACC CGTACCTGCG CCTAAAAGTG CGAAGAGACC ACATCATAGA TGATGCTCTC 
GTCAGGCTGG AGATGATTGC CATGGAAAAT CCAGCAGACT TGAAGAAGCA GTTGTATGTG 
GAGTTTGAAG GAGAGCAGGG GGTGGATGAA GGAGGAGTTT CCAAAGAGTT CTTTCAGCTG 
GTAGTAGAAG AGATTTTCAA CCCTGATATA GGCATGTTCA CCTATGATGA AAGCACAAAG 
CTTTTCTGGT TTAACCCCTC ATCATTTGAA AATGAAGGCC AGTTCACATT GATAGGTATT 
GTCCTGGGGC TAGCTATCTA CAATAACTGC ATTCTGGATG TGCACTTCCC TATGGTTGTT 
TACAGAAAGC TAATGGGAAA GAAAGGAACC TTCCGAGACC TTGCAGACTC TCATCCGGTT 
CTTTATCAAA GTCTGAAGGA GCTGTTGGAA TATGAGGGCA ATGTAGAAGA AGACATGATG 
ATCACCTTCC AGATATCACA AACAGACTTG TTTGGAAACC CTCTCATGTA CGATTTAAAG 
GAAGGTGGAG ACAAAATCCC TGTCACCAAT GAAAACAGGA AGGAGTTTGT AGCATTATAT 
GCTGAATATA TGCTGAACAA AAGTGTGGAG AAACAATTCA AGGCTTTCAG AAGAGGTTTC 
CATATGGTTA CTAACGAGTC TCCGTTGAAG TATCTGTTTC GACCTGAAGA AATTGAGCTG 
CTTATATGTG GAAGCAGAAA TCTGGACTTT CAAGCACTCG AAGAGACTAC GGAATATGAT 
GGCGGTTACA GCAGGGATTG TCGCATTATT AAGGACTTCT GGGAGACAGT GCACTCATTT 
GGACAGGAGC AGAAGAGGTT GTTTCTGCAG TTCACCACAG GCACAGACAG AGCGCCAGTG 
GGCGGCCTGG GCAAGTTGAA GATGATCATT GCCAAAAATG GGCCAGACTC AGACAGGTTA 
CCAACCTCTC ACACCTGCTT CAACGTGCTG CTTCTTCCTG AATACTCCAC CAAGGAAAAA 
CTTAGAGAGA GACTCCTCAA AGCCATCACT TATGCCAAAG GCTTCGGCAT GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007249175.2
CDS	267..2843
Translation	MKRAAAKHLIERYYHQLTEGCGNEACTNEACGSSPGFRRMDNNAAAIKALELYKNNAKLCDPHPSKKGASSAFPENSAKGAHNFSACSNGKMNHKDLPPSREDFRDLNYLTEEKVYEILALCGETEDYSPLIRVIGRVFSSAESLVQSFRKAKQHTKEELKSLQAKDEDKDEDEKENASGSASAMEVDPDAAAASSGEGASHDGNNVQKLGPVEVSVDIDAVRRVYDRLLSNEKIEAAFLNALVYLSPNVECDLTYHNVYAADPNYLNVFIIVMENSNLHSPEYLEIALPQFCKAMSKLPLPALAKLARLWSQYGADQIRRLVETFQQLITYTVISNEFNSDNLVNEDDGVVAATKCLKIVYYANVLGGDLDTDHNEEEDDEPIPESSELTLQELLGEERRNKKGPRVDPLETELGIRTSDCRKPLIPFEEFINEPLNDVLEMDKDYTFFKVETESKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITALYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFENEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLADSHPVLYQSLKELLEYEGNVEEDMMITFQISQTDLFGNPLMYDLKEGGDKIPVTNENRKEFVALYAEYMLNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYSRDCRIIKDFWETVHSFGQEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDSDRLPTSHTCFNVLLLPEYSTKEKLRERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_007249113.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus ubiquitin protein ligase E3A (ube3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007249113.2

ORF Insert Sequence:

ATGAAGCGAG CGGCTGCAAA GCATCTAATT GAGCGTTACT ACCACCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAACGAGGCT TGTGGCTCCT CTCCGGGTTT CCGGCGCATG 
GATAACAATG CAGCGGCCAT CAAGGCCCTG GAACTCTATA AGAATAACGC CAAACTCTGT 
GATCCTCACC CCTCCAAGAA AGGTGCCAGC TCGGCTTTCC CGGAGAACAG TGCCAAAGGA 
GCTCACAACT TTTCTGCTTG CAGCAACGGG AAGATGAACC ACAAGGATCT GCCTCCCTCA 
AGGGAAGACT TTAGAGATTT GAACTATTTG ACGGAGGAGA AGGTTTATGA AATTCTGGCT 
CTCTGTGGTG AGACAGAAGA CTACTCCCCT CTAATCCGTG TCATCGGGAG GGTCTTCTCT 
AGTGCTGAAA GCTTAGTGCA GAGCTTCCGG AAGGCTAAAC AACACACCAA GGAGGAGCTG 
AAATCGCTGC AGGCCAAGGA CGAGGACAAG GATGAAGATG AGAAAGAAAA CGCCTCTGGT 
TCGGCCAGTG CCATGGAAGT GGATCCTGAT GCTGCTGCCG CTTCCAGTGG AGAGGGGGCC 
TCTCATGATG GTAATAATGT GCAAAAGCTG GGCCCGGTTG AAGTGTCTGT GGACATTGAC 
GCAGTAAGAA GAGTTTATGA CAGACTATTG TCAAACGAGA AGATTGAGGC GGCATTCCTC 
AACGCATTAG TGTATCTGTC GCCCAATGTG GAATGCGACC TCACGTATCA CAACGTGTAT 
GCCGCAGACC CCAATTATCT AAACGTGTTC ATCATTGTCA TGGAGAACAG CAACCTGCAC 
AGCCCTGAGT ACCTAGAGAT TGCCCTGCCC CAGTTTTGCA AGGCCATGAG CAAGCTACCT 
CTCCCAGCTC TGGCCAAGCT AGCACGTCTT TGGTCGCAGT ACGGAGCAGA CCAGATAAGG 
CGCCTGGTGG AGACCTTCCA GCAGCTCATC ACCTACACAG TTATAAGCAA CGAGTTCAAC 
AGTGACAACC TGGTAAATGA GGATGACGGC GTGGTGGCGG CCACCAAGTG CTTGAAAATC 
GTCTACTATG CAAACGTGCT GGGCGGTGAC CTGGACACAG ACCACAACGA GGAGGAGGAT 
GACGAGCCCA TCCCTGAGTC CAGTGAGCTG ACCCTACAGG AGCTGCTGGG GGAGGAGCGA 
CGCAACAAGA AAGGGCCGCG TGTGGACCCG CTTGAGACTG AGCTGGGCAT TCGCACCTCG 
GACTGCCGCA AGCCCCTCAT TCCCTTCGAG GAGTTCATCA ATGAGCCGCT AAACGATGTG 
CTGGAAATGG ACAAGGACTA CACCTTCTTC AAGGTGGAGA CGGAGAGCAA GTTCTCCTTC 
ATGACCTGTC CATTCATCCT TAACGCTGTG ACAAAAAACC TGGGCCTCTA CTATGACAAC 
CGGATCCGCA TGTACAGTGA GCGCCGAATC ACTGCTCTCT ACAGCCTGGT GCAAGGACAG 
CAGCTCAACC CGTACCTGCG CCTAAAAGTG CGAAGAGACC ACATCATAGA TGATGCTCTC 
GTCAGGCTGG AGATGATTGC CATGGAAAAT CCAGCAGACT TGAAGAAGCA GTTGTATGTG 
GAGTTTGAAG GAGAGCAGGG GGTGGATGAA GGAGGAGTTT CCAAAGAGTT CTTTCAGCTG 
GTAGTAGAAG AGATTTTCAA CCCTGATATA GGCATGTTCA CCTATGATGA AAGCACAAAG 
CTTTTCTGGT TTAACCCCTC ATCATTTGAA AATGAAGGCC AGTTCACATT GATAGGTATT 
GTCCTGGGGC TAGCTATCTA CAATAACTGC ATTCTGGATG TGCACTTCCC TATGGTTGTT 
TACAGAAAGC TAATGGGAAA GAAAGGAACC TTCCGAGACC TTGCAGACTC TCATCCGGTT 
CTTTATCAAA GTCTGAAGGA GCTGTTGGAA TATGAGGGCA ATGTAGAAGA AGACATGATG 
ATCACCTTCC AGATATCACA AACAGACTTG TTTGGAAACC CTCTCATGTA CGATTTAAAG 
GAAGGTGGAG ACAAAATCCC TGTCACCAAT GAAAACAGGA AGGAGTTTGT AGCATTATAT 
GCTGAATATA TGCTGAACAA AAGTGTGGAG AAACAATTCA AGGCTTTCAG AAGAGGTTTC 
CATATGGTTA CTAACGAGTC TCCGTTGAAG TATCTGTTTC GACCTGAAGA AATTGAGCTG 
CTTATATGTG GAAGCAGAAA TCTGGACTTT CAAGCACTCG AAGAGACTAC GGAATATGAT 
GGCGGTTACA GCAGGGATTG TCGCATTATT AAGGACTTCT GGGAGACAGT GCACTCATTT 
GGACAGGAGC AGAAGAGGTT GTTTCTGCAG TTCACCACAG GCACAGACAG AGCGCCAGTG 
GGCGGCCTGG GCAAGTTGAA GATGATCATT GCCAAAAATG GGCCAGACTC AGACAGGTTA 
CCAACCTCTC ACACCTGCTT CAACGTGCTG CTTCTTCCTG AATACTCCAC CAAGGAAAAA 
CTTAGAGAGA GACTCCTCAA AGCCATCACT TATGCCAAAG GCTTCGGCAT GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3a ( XM_007249113.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007249175.1 Astyanax mexicanus ubiquitin protein ligase E3A (ube3a), mRNA.

Price & Availability↑

CloneID	OAs31664
Clone ID Related Accession (Same CDS sequence)	XM_007249113.1
Accession Version	XM_007249113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-10
Organism	Astyanax mexicanus(Mexican tetra)
Product	ubiquitin-protein ligase E3A
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749309.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGAG CGGCTGCAAA GCATCTAATT GAGCGTTACT ACCACCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAACGAGGCT TGTGGCTCCT CTCCGGGTTT CCGGCGCATG 
GATAACAATG CAGCGGCCAT CAAGGCCCTG GAGCTCTATA AGAATAACGC CAAACTCTGT 
GATCCTCACC CCTCCAAGAA AGGTGCCAGC TCGGCTTTCC CGGAGAACAG TGCCAAAGGA 
GCTCACAACT TTTCTGCTTG CAGCAACGGG AAGATGAACC ACAAGGATCT GCCTCCCTCA 
AGGGAAGACT TTAGAGATTT GAACTATTTG ACGGAGGAGA AGGTTTATGA AATTCTGGCT 
CTCTGTGGTG AGACAGAAGA CTACTCCCCT CTAATCCGTG TCATCGGGAG GGTCTTCTCT 
AGTGCTGAAA GCTTAGTGCA GAGCTTCCGG AAGGCTAAAC AACACACCAA GGAGGAGCTG 
AAATCGCTGC AGGCCAAGGA CGAGGACAAG GATGAAGATG AGAAAGAAAA CGCCTCTGGT 
TCGGCCAGTG CCATGGAAGT GGATCCTGAT GCTGCTGCCG CTTCCAGTGG AGAGGGGGCC 
TCTCATGATG GTAATAATGT GCAAAAGCTG GGCCCGGTTG AAGTGTCTGT GGACATTGAC 
GCAGTAAGAA GAGTTTATGA CAGACTATTG TCAAACGAGA AGATTGAGGC GGCATTCCTC 
AACGCATTAG TGTATCTGTC GCCCAATGTG GAATGCGACC TCACGTATCA CAACGTGTAT 
GCCGCAGACC CCAATTATCT AAACGTGTTC ATCATTGTCA TGGAGAACAG CAACCTGCAC 
AGCCCTGAGT ACCTAGAGAT TGCCCTGCCC CAGTTTTGCA AGGCCATGAG CAAGCTACCT 
CTCCCAGCTC TGGCCAAGCT AGCACGTCTT TGGTCGCAGT ACGGAGCAGA CCAGATCAGG 
CGCCTGGTGG AGACCTTCCA GCAGCTCATC ACCTACACAG TTATAAGCAA CGAGTTCAAC 
AGTGACAACC TGGTAAATGA GGATGACGGC GTGGTGGCGG CCACCAAGTG CTTGAAAATC 
GTCTACTATG CAAACGTGCT GGGCGGTGAC CTGGACACAG ACCACAACGA GGAGGAGGAT 
GACGAGCCCA TCCCTGAGTC CAGTGAGCTG ACCCTACAGG AGCTGCTGGG GGAGGAGCGA 
CGCAACAAGA AAGGGCCGCG TGTGGACCCG CTTGAGACTG AGCTGGGCAT TCGCACCTCG 
GACTGCCGCA AGCCCCTCAT TCCCTTCGAG GAGTTCATCA ATGAGCCGCT AAACGATGTG 
CTGGAAATGG ACAAGGACTA CACCTTCTTC AAGGTGGAGA CGGAGAGCAA GTTCTCCTTC 
ATGACCTGTC CATTCATCCT TAACGCTGTG ACAAAAAACC TGGGCCTCTA CTATGACAAC 
CGGATCCGCA TGTACAGTGA GCGCCGAATC ACTGCTCTCT ACAGCCTGGT GCAAGGACAG 
CAGCTCAACC CGTACCTGCG CCTAAAAGTG CGAAGAGACC ACATCATAGA TGATGCTCTC 
GTCAGGCTGG AGATGATTGC CATGGAAAAT CCAGCAGACT TGAAGAAGCA GTTGTATGTG 
GAGTTTGAAG GAGAGCAGGG GGTGGATGAA GGAGGAGTTT CCAAAGAGTT CTTTCAGCTG 
GTAGTAGAAG AGATTTTCAA CCCTGATATA GGCATGTTCA CCTATGATGA AAGCACAAAG 
CTTTTCTGGT TTAACCCCTC ATCATTTGAA AATGAAGGCC AGTTCACATT GATAGGTATT 
GTCCTGGGGC TAGCTATCTA CAATAACTGC ATTCTGGATG TGCACTTCCC TATGGTTGTT 
TACAGAAAGC TAATGGGAAA GAAAGGAACC TTCCGAGACC TTGCAGACTC TCATCCGGTT 
CTTTATCAAA GTCTGAAGGA GCTGTTGGAA TATGAGGGCA ATGTAGAAGA AGACATGATG 
ATCACCTTCC AGATATCACA AACAGACTTG TTTGGAAACC CTCTCATGTA CGATTTAAAG 
GAAGGTGGAG ACAAAATCCC TGTCACCAGT GAAAACAGGA AGGAGTTTGT AGCATTATAT 
GCTGAATATA TGCTGAACAA AAGTGTGGAG AAACAATTCA AGGCTTTCAG AAGAGGTTTC 
CATATGGTTA CTAACGAGTC TCCGTTGAAG TATCTGTTTC GACCTGAAGA AATTGAGCTG 
CTTATATGTG GAAGCAGAAA TCTGGACTTT CAAGCACTCG AAGAGACTAC GGAATATGAT 
GGCGGTTACA GCAGGGATTG TCGCATTATT AAGGACTTCT GGGAGACAGT GCACTCATTT 
GGACAGGAGC AGAAGAGGTT GTTTCTGCAG TTCACCACAG GCACAGACAG AGCGCCAGTG 
GGCGGCCTGG GCAAGTTGAA GATGATCATT GCCAAAAATG GGCCAGACTC AGACAGGTTA 
CCAACCTCTC ACACCTGCTT CAACGTGCTG CTTCTTCCTG AATACTCCAC CAAGGAAAAA 
CTTAGAGAGA GACTCCTCAA AGCCATCACT TATGCCAAAG GCTTCGGCAT GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007249175.1
CDS	250..2826
Translation	MKRAAAKHLIERYYHQLTEGCGNEACTNEACGSSPGFRRMDNNAAAIKALELYKNNAKLCDPHPSKKGASSAFPENSAKGAHNFSACSNGKMNHKDLPPSREDFRDLNYLTEEKVYEILALCGETEDYSPLIRVIGRVFSSAESLVQSFRKAKQHTKEELKSLQAKDEDKDEDEKENASGSASAMEVDPDAAAASSGEGASHDGNNVQKLGPVEVSVDIDAVRRVYDRLLSNEKIEAAFLNALVYLSPNVECDLTYHNVYAADPNYLNVFIIVMENSNLHSPEYLEIALPQFCKAMSKLPLPALAKLARLWSQYGADQIRRLVETFQQLITYTVISNEFNSDNLVNEDDGVVAATKCLKIVYYANVLGGDLDTDHNEEEDDEPIPESSELTLQELLGEERRNKKGPRVDPLETELGIRTSDCRKPLIPFEEFINEPLNDVLEMDKDYTFFKVETESKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITALYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFENEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLADSHPVLYQSLKELLEYEGNVEEDMMITFQISQTDLFGNPLMYDLKEGGDKIPVTSENRKEFVALYAEYMLNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYSRDCRIIKDFWETVHSFGQEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDSDRLPTSHTCFNVLLLPEYSTKEKLRERLLKAITYAKGFGML

Target ORF information:

RefSeq Version	XM_007249113.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus ubiquitin protein ligase E3A (ube3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007249113.1

ORF Insert Sequence:

ATGAAGCGAG CGGCTGCAAA GCATCTAATT GAGCGTTACT ACCACCAGTT AACTGAGGGC 
TGTGGAAATG AGGCCTGCAC GAACGAGGCT TGTGGCTCCT CTCCGGGTTT CCGGCGCATG 
GATAACAATG CAGCGGCCAT CAAGGCCCTG GAGCTCTATA AGAATAACGC CAAACTCTGT 
GATCCTCACC CCTCCAAGAA AGGTGCCAGC TCGGCTTTCC CGGAGAACAG TGCCAAAGGA 
GCTCACAACT TTTCTGCTTG CAGCAACGGG AAGATGAACC ACAAGGATCT GCCTCCCTCA 
AGGGAAGACT TTAGAGATTT GAACTATTTG ACGGAGGAGA AGGTTTATGA AATTCTGGCT 
CTCTGTGGTG AGACAGAAGA CTACTCCCCT CTAATCCGTG TCATCGGGAG GGTCTTCTCT 
AGTGCTGAAA GCTTAGTGCA GAGCTTCCGG AAGGCTAAAC AACACACCAA GGAGGAGCTG 
AAATCGCTGC AGGCCAAGGA CGAGGACAAG GATGAAGATG AGAAAGAAAA CGCCTCTGGT 
TCGGCCAGTG CCATGGAAGT GGATCCTGAT GCTGCTGCCG CTTCCAGTGG AGAGGGGGCC 
TCTCATGATG GTAATAATGT GCAAAAGCTG GGCCCGGTTG AAGTGTCTGT GGACATTGAC 
GCAGTAAGAA GAGTTTATGA CAGACTATTG TCAAACGAGA AGATTGAGGC GGCATTCCTC 
AACGCATTAG TGTATCTGTC GCCCAATGTG GAATGCGACC TCACGTATCA CAACGTGTAT 
GCCGCAGACC CCAATTATCT AAACGTGTTC ATCATTGTCA TGGAGAACAG CAACCTGCAC 
AGCCCTGAGT ACCTAGAGAT TGCCCTGCCC CAGTTTTGCA AGGCCATGAG CAAGCTACCT 
CTCCCAGCTC TGGCCAAGCT AGCACGTCTT TGGTCGCAGT ACGGAGCAGA CCAGATCAGG 
CGCCTGGTGG AGACCTTCCA GCAGCTCATC ACCTACACAG TTATAAGCAA CGAGTTCAAC 
AGTGACAACC TGGTAAATGA GGATGACGGC GTGGTGGCGG CCACCAAGTG CTTGAAAATC 
GTCTACTATG CAAACGTGCT GGGCGGTGAC CTGGACACAG ACCACAACGA GGAGGAGGAT 
GACGAGCCCA TCCCTGAGTC CAGTGAGCTG ACCCTACAGG AGCTGCTGGG GGAGGAGCGA 
CGCAACAAGA AAGGGCCGCG TGTGGACCCG CTTGAGACTG AGCTGGGCAT TCGCACCTCG 
GACTGCCGCA AGCCCCTCAT TCCCTTCGAG GAGTTCATCA ATGAGCCGCT AAACGATGTG 
CTGGAAATGG ACAAGGACTA CACCTTCTTC AAGGTGGAGA CGGAGAGCAA GTTCTCCTTC 
ATGACCTGTC CATTCATCCT TAACGCTGTG ACAAAAAACC TGGGCCTCTA CTATGACAAC 
CGGATCCGCA TGTACAGTGA GCGCCGAATC ACTGCTCTCT ACAGCCTGGT GCAAGGACAG 
CAGCTCAACC CGTACCTGCG CCTAAAAGTG CGAAGAGACC ACATCATAGA TGATGCTCTC 
GTCAGGCTGG AGATGATTGC CATGGAAAAT CCAGCAGACT TGAAGAAGCA GTTGTATGTG 
GAGTTTGAAG GAGAGCAGGG GGTGGATGAA GGAGGAGTTT CCAAAGAGTT CTTTCAGCTG 
GTAGTAGAAG AGATTTTCAA CCCTGATATA GGCATGTTCA CCTATGATGA AAGCACAAAG 
CTTTTCTGGT TTAACCCCTC ATCATTTGAA AATGAAGGCC AGTTCACATT GATAGGTATT 
GTCCTGGGGC TAGCTATCTA CAATAACTGC ATTCTGGATG TGCACTTCCC TATGGTTGTT 
TACAGAAAGC TAATGGGAAA GAAAGGAACC TTCCGAGACC TTGCAGACTC TCATCCGGTT 
CTTTATCAAA GTCTGAAGGA GCTGTTGGAA TATGAGGGCA ATGTAGAAGA AGACATGATG 
ATCACCTTCC AGATATCACA AACAGACTTG TTTGGAAACC CTCTCATGTA CGATTTAAAG 
GAAGGTGGAG ACAAAATCCC TGTCACCAGT GAAAACAGGA AGGAGTTTGT AGCATTATAT 
GCTGAATATA TGCTGAACAA AAGTGTGGAG AAACAATTCA AGGCTTTCAG AAGAGGTTTC 
CATATGGTTA CTAACGAGTC TCCGTTGAAG TATCTGTTTC GACCTGAAGA AATTGAGCTG 
CTTATATGTG GAAGCAGAAA TCTGGACTTT CAAGCACTCG AAGAGACTAC GGAATATGAT 
GGCGGTTACA GCAGGGATTG TCGCATTATT AAGGACTTCT GGGAGACAGT GCACTCATTT 
GGACAGGAGC AGAAGAGGTT GTTTCTGCAG TTCACCACAG GCACAGACAG AGCGCCAGTG 
GGCGGCCTGG GCAAGTTGAA GATGATCATT GCCAAAAATG GGCCAGACTC AGACAGGTTA 
CCAACCTCTC ACACCTGCTT CAACGTGCTG CTTCTTCCTG AATACTCCAC CAAGGAAAAA 
CTTAGAGAGA GACTCCTCAA AGCCATCACT TATGCCAAAG GCTTCGGCAT GCTCTGA