UBC35 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBC35
Entrez Gene ID	844224
Full Name	ubiquitin-conjugating enzyme 35
Synonyms	F9K20.8,F9K20_8,UBC13A,UBIQUITIN CONJUGATING ENZYME 13A,ubiquitin-conjugating enzyme 35
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001334851.1	NP_001321618.1	ubiquitin-conjugating enzyme 35
NM_106535.4	NP_565192.1	ubiquitin-conjugating enzyme 35
NM_001036221.3	NP_001031298.1	ubiquitin-conjugating enzyme 35
NM_001334850.1	NP_001319409.1	ubiquitin-conjugating enzyme 35

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_02568	XP_003721235.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005197	XP_314098.2
Saccharomyces cerevisiae (baker's yeast)	UBC13	NP_010377.3
Eremothecium gossypii	AGOS_AGR121C	NP_986787.1
Neurospora crassa	NCU02113	XP_964107.1
Canis lupus familiaris (dog)	LOC607817	XP_003433156.1
Schizosaccharomyces pombe (fission yeast)	ubc13	NP_594929.1
Oryza sativa (rice)	Os01g0673600	NP_001043834.1
Drosophila melanogaster (fruit fly)	ben	NP_001162752.1
Kluyveromyces lactis	KLLA0C17622g	XP_452987.1
Arabidopsis thaliana (thale cress)	UBC35	NP_565192.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

UBC13 activity is critical for all major aspects of root development. The double mutant plants were insensitive to auxin treatments and the ubc13 mutant had a reduced auxin response.
Title: UBC13, an E2 enzyme for Lys63-linked ubiquitination, functions in root development by affecting auxin signaling and Aux/IAA protein stability.

Arabidopsis contains two highly conserved and likely duplicated UBC13 genes, AtUBC13A and AtUBC13B. Data suggests the existence of an error-free DNA damage tolerance pathway in plants. [UBC13A]
Title: Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants.

The following UBC35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBC35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb31268	NM_106535.4 Latest version!	Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OAb31269	NM_001036221.3 Latest version!	Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OAb32837	NM_001334850.1 Latest version!	Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OAb34900	NM_001334851.1 Latest version!	Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBC35 ( NM_106535.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_565192.1 Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.

Price & Availability↑

CloneID	OAb31268
Clone ID Related Accession (Same CDS sequence)	NM_106535.4
Accession Version	NM_106535.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ubiquitin-conjugating enzyme 35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_106535.3.

ATGGCCAACA GTAATTTGCC GCGAAGAATC ATCAAGGAAA CTCAACGTCT GCTCAGTGAA 
CCGGCTCCCG GTATAAGTGC CTCTCCATCT GAGGATAATA TGCGGTATTT CAACGTTATG 
ATTCTTGGTC CTACACAATC ACCTTATGAA GGAGGAGTTT TCAAGTTGGA GCTCTTTTTG 
CCTGAAGAAT ACCCTATGGC AGCTCCCAAG GTTAGGTTTC TCACAAAGAT ATACCATCCT 
AACATTGACA AGCTTGGAAG AATCTGTCTT GATATTCTCA AGGACAAATG GAGCCCTGCA 
CTACAAATAC GAACAGTGCT CTTAAGTATT CAAGCTCTTC TGAGTGCACC AAACCCAGAT 
GATCCGCTTT CTGAGAACAT TGCTAAGCAC TGGAAGAGTA ATGAGGCAGA AGCTGTGGAT 
ACAGCTAAGG AATGGACCCG TCTTTATGCA AGCGGCGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_565192.1
CDS	214..675
Translation	MANSNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAKEWTRLYASGA

Target ORF information:

RefSeq Version	NM_106535.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_106535.4

ORF Insert Sequence:

ATGGCCAACA GTAATTTGCC GCGAAGAATC ATCAAGGAAA CTCAACGTCT GCTCAGTGAA 
CCGGCTCCCG GTATAAGTGC CTCTCCATCT GAGGATAATA TGCGGTATTT CAACGTTATG 
ATTCTTGGTC CTACACAATC ACCTTATGAA GGAGGAGTTT TCAAGTTGGA GCTCTTTTTG 
CCTGAAGAAT ACCCTATGGC AGCTCCCAAG GTTAGGTTTC TCACAAAGAT ATACCATCCT 
AACATTGACA AGCTTGGAAG AATCTGTCTT GATATTCTCA AGGACAAATG GAGCCCTGCA 
CTACAAATAC GAACAGTGCT CTTAAGTATT CAAGCTCTTC TGAGTGCACC AAACCCAGAT 
GATCCGCTTT CTGAGAACAT TGCTAAGCAC TGGAAGAGTA ATGAGGCAGA AGCTGTGGAT 
ACAGCTAAGG AATGGACCCG TCTTTATGCA AGCGGCGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBC35 ( NM_001036221.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001031298.1 Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.

Price & Availability↑

CloneID	OAb31269
Clone ID Related Accession (Same CDS sequence)	NM_001036221.3
Accession Version	NM_001036221.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ubiquitin-conjugating enzyme 35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_001036221.2.

ATGGCCAACA GTAATTTGCC GCGAAGAATC ATCAAGGAAA CTCAACGTCT GCTCAGTGAA 
CCGGCTCCCG GTATAAGTGC CTCTCCATCT GAGGATAATA TGCGGTATTT CAACGTTATG 
ATTCTTGGTC CTACACAATC ACCTTATGAA GGAGGAGTTT TCAAGTTGGA GCTCTTTTTG 
CCTGAAGAAT ACCCTATGGC AGCTCCCAAG GTTAGGTTTC TCACAAAGAT ATACCATCCT 
AACATTGACA AGCTTGGAAG AATCTGTCTT GATATTCTCA AGGACAAATG GAGCCCTGCA 
CTACAAATAC GAACAGTGCT CTTAAGGTAT ATGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001031298.1
CDS	156..494
Translation	MANSNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLRYMS

Target ORF information:

RefSeq Version	NM_001036221.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001036221.3

ORF Insert Sequence:

ATGGCCAACA GTAATTTGCC GCGAAGAATC ATCAAGGAAA CTCAACGTCT GCTCAGTGAA 
CCGGCTCCCG GTATAAGTGC CTCTCCATCT GAGGATAATA TGCGGTATTT CAACGTTATG 
ATTCTTGGTC CTACACAATC ACCTTATGAA GGAGGAGTTT TCAAGTTGGA GCTCTTTTTG 
CCTGAAGAAT ACCCTATGGC AGCTCCCAAG GTTAGGTTTC TCACAAAGAT ATACCATCCT 
AACATTGACA AGCTTGGAAG AATCTGTCTT GATATTCTCA AGGACAAATG GAGCCCTGCA 
CTACAAATAC GAACAGTGCT CTTAAGGTAT ATGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBC35 ( NM_001334850.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319409.1 Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.

Price & Availability↑

CloneID	OAb32837
Clone ID Related Accession (Same CDS sequence)	NM_001334850.1
Accession Version	NM_001334850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ubiquitin-conjugating enzyme 35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGCGGTATT TCAACGTTAT GATTCTTGGT CCTACACAAT CACCTTATGA AGGTAGAGGA 
GTTTTCAAGT TGGAGCTCTT TTTGCCTGAA GAATACCCTA TGGCAGCTCC CAAGGTTAGG 
TTTCTCACAA AGATATACCA TCCTAACATT GACAAGCTTG GAAGAATCTG TCTTGATATT 
CTCAAGGACA AATGGAGCCC TGCACTACAA ATACGAACAG TGCTCTTAAG TATTCAAGCT 
CTTCTGAGTG CACCAAACCC AGATGATCCG CTTTCTGAGA ACATTGCTAA GCACTGGAAG 
AGTAATGAGG CAGAAGCTGT GGATACAGCT AAGGAATGGA CCCGTCTTTA TGCAAGCGGC 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319409.1
CDS	444..809
Translation	MRYFNVMILGPTQSPYEGRGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAKEWTRLYASGA

Target ORF information:

RefSeq Version	NM_001334850.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001334850.1

ORF Insert Sequence:

ATGCGGTATT TCAACGTTAT GATTCTTGGT CCTACACAAT CACCTTATGA AGGTAGAGGA 
GTTTTCAAGT TGGAGCTCTT TTTGCCTGAA GAATACCCTA TGGCAGCTCC CAAGGTTAGG 
TTTCTCACAA AGATATACCA TCCTAACATT GACAAGCTTG GAAGAATCTG TCTTGATATT 
CTCAAGGACA AATGGAGCCC TGCACTACAA ATACGAACAG TGCTCTTAAG TATTCAAGCT 
CTTCTGAGTG CACCAAACCC AGATGATCCG CTTTCTGAGA ACATTGCTAA GCACTGGAAG 
AGTAATGAGG CAGAAGCTGT GGATACAGCT AAGGAATGGA CCCGTCTTTA TGCAAGCGGC 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBC35 ( NM_001334851.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001321618.1 Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.

Price & Availability↑

CloneID	OAb34900
Clone ID Related Accession (Same CDS sequence)	NM_001334851.1
Accession Version	NM_001334851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ubiquitin-conjugating enzyme 35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGCGGTATT TCAACGTTAT GATTCTTGGT CCTACACAAT CACCTTATGA AGGAGGAGTT 
TTCAAGTTGG AGCTCTTTTT GCCTGAAGAA TACCCTATGG CAGCTCCCAA GGTTAGGTTT 
CTCACAAAGA TATACCATCC TAACATTGAC AAGCTTGGAA GAATCTGTCT TGATATTCTC 
AAGGACAAAT GGAGCCCTGC ACTACAAATA CGAACAGTGC TCTTAAGTAT TCAAGCTCTT 
CTGAGTGCAC CAAACCCAGA TGATCCGCTT TCTGAGAACA TTGCTAAGCA CTGGAAGAGT 
AATGAGGCAG AAGCTGTGGA TACAGCTAAG GAATGGACCC GTCTTTATGC AAGCGGCGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001321618.1
CDS	484..846
Translation	MRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVDTAKEWTRLYASGA

Target ORF information:

RefSeq Version	NM_001334851.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ubiquitin-conjugating enzyme 35 (UBC35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001334851.1

ORF Insert Sequence:

ATGCGGTATT TCAACGTTAT GATTCTTGGT CCTACACAAT CACCTTATGA AGGAGGAGTT 
TTCAAGTTGG AGCTCTTTTT GCCTGAAGAA TACCCTATGG CAGCTCCCAA GGTTAGGTTT 
CTCACAAAGA TATACCATCC TAACATTGAC AAGCTTGGAA GAATCTGTCT TGATATTCTC 
AAGGACAAAT GGAGCCCTGC ACTACAAATA CGAACAGTGC TCTTAAGTAT TCAAGCTCTT 
CTGAGTGCAC CAAACCCAGA TGATCCGCTT TCTGAGAACA TTGCTAAGCA CTGGAAGAGT 
AATGAGGCAG AAGCTGTGGA TACAGCTAAG GAATGGACCC GTCTTTATGC AAGCGGCGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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UBC35 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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