VPS35B cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS35B
Entrez Gene ID	843918
Full Name	VPS35 homolog B
Synonyms	T4O12.9,T4O12_9,VPS35 homolog B
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001334699.1	NP_001321274.1	VPS35 homolog B
NM_106235.5	NP_177713.3	VPS35 homolog B

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS35	NP_060676.2
Canis lupus familiaris (dog)	VPS35	XP_532570.3
Eremothecium gossypii	AGOS_AEL107W	NP_984754.1
Arabidopsis thaliana (thale cress)	VPS35B	NP_177713.3
Rattus norvegicus (Norway rat)	Vps35	NP_001099188.2
Danio rerio (zebrafish)	vps35	NP_001020688.2
	VPS35	XP_001108828.2
Gallus gallus (chicken)	VPS35	NP_001005842.1
Caenorhabditis elegans (roundworm)	vps-35	NP_495180.2
Saccharomyces cerevisiae (baker's yeast)	VPS35	NP_012381.1
Kluyveromyces lactis	KLLA0B07535g	XP_451864.1
Magnaporthe oryzae (rice blast fungus)	MGG_05089	XP_003712611.1
Bos taurus (cattle)	VPS35	NP_001039723.1
Drosophila melanogaster (fruit fly)	Vps35	NP_611651.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007683	XP_308188.4
Schizosaccharomyces pombe (fission yeast)	vps35	NP_588260.2
Oryza sativa (rice)	Os03g0801600	NP_001051593.1
Pan troglodytes (chimpanzee)	VPS35	XP_001161536.1
Mus musculus (house mouse)	Vps35	NP_075373.1
Neurospora crassa	NCU06284	XP_962546.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggest that VPS35, mainly VPS35b, is involved in sorting proteins to PSVs in seeds, possibly by recycling VSR from PVCs to the Golgi complex, and is also involved in plant growth and senescence in vegetative organs
Title: Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence.

The following VPS35B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb28072	NM_106235.5 Latest version!	Arabidopsis thaliana VPS35 homolog B (VPS35B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb34820	NM_001334699.1 Latest version!	Arabidopsis thaliana VPS35 homolog B (VPS35B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS35B ( NM_106235.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_177713.3 Arabidopsis thaliana VPS35 homolog B (VPS35B), mRNA.

Price & Availability↑

CloneID	OAb28072
Clone ID Related Accession (Same CDS sequence)	NM_106235.5
Accession Version	NM_106235.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	VPS35 homolog B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_106235.4.

ATGAGAACGC TCGCCGGAGT AGAAGACGAG GATAAGTGGC TGGCGGAAGG AATCGCCGGA 
ATCCAGCACA ACGCTTTCTT CATGCATCGC GCTTTGGACG CGAATAATCT CAGAGAAGTT 
CTCAAGTACT CTGCTCTGAT GTTATCGGAG CTTCGAACCT CCAAGCTCTC TCCTCAGAAA 
TATTACGATC TCTATATGAG AGCTTTCGAT CAATTGAGGC AGCTGGAGAT TTTCTTTAAG 
GATGAGAGTA GGCATGGATT ACCTGTTGTT GATTTGTATG AACTTGTTCA ACATGCCGGC 
AACATATTGC CCAGAATGTA TCTGCTATGT ACGGTCGGGT CTGTTTATAT CAAAAGTAAG 
CAGGCTCCTT CGAAGGATGT ACTTAAGGAT CTTGTGGAAA TGTGTCGAGG TGTTCAACAT 
CCTATTCGTG GCCTCTTTTT GAGGAGTTAT CTCGCTCAAG TGAGCAGGGA TAAGTTACCT 
GAGATTGGCT CAGACTACGA AGGAGATGCA AATACTGTGA TGGATGCTGT GGAGTTTGTG 
CTACAAAATT TCACAGAGAT GAATAAGCTT TGGGTTCGAA TACAGCATCA GGGACCAGGA 
ACAGTTCGAG AAAAGCAGGA GAAAGAGAGG AACGAACTTC GTGATCTTGT TGGAAAAAAT 
CTGCATGTTC TAGGACAGAT AGAAGGTGTT GACCTTGAGA TGTACAAAGA GACTGTTCTT 
CCTAGGGTCT TAGAGCAGGT CGTCAACTGT AAAGATAAGT TGGCTCAGTA TTATCTGATG 
GAGTGCATAA TTCAAGTCTT TCCTGATGAG TACCATTTGC AGACTCTTGA GACCTTATTG 
GCTGCTTGTA CCCAACTGAT GCCAACTGTT GACACGAAGA TAGTGTTAAC CCAACTAATG 
GACCGGTTAT CAAACTATGC TGCCTCAAGT CCAGATGTGT TACACGAATT TTTGCAAGTG 
GAAGCTTTTG CTAAATTGAG CAATGCAATT GGAAAGGTGA TAGATACACA GCTTGAGATG 
CCTATTGTCG GAGCTATGAC TCTGTTTGTC TCTCTTTTGA CTTTTACTCT CCGAGTTCAT 
CCTGATCGGC TTGACTATGT GGATCAAGTA TTGGGTGCAT GTGTAGTTAA GCTTTCCAGT 
GTGCCCAAGC TGGAGGACGC CCGTGCAATG AAACAAGTAG TGGCACTTTT GAGTGCTCCT 
TTAGAAAAGT ACAGTGACAT AGTTACAGCT CTTACATTGT CAAATTATCC GCGTGTCATG 
GATCATCTTG ATGATGGAAC GAACAAAGTA ATGGCTATGC TAATTATTCA AAGTATAATG 
AAAACTGATT CGTGTATATC AACTGCTGAT AAGGTTGAGG TGCTGTTTGA ATTGATAAAA 
GGACTTATTA AAGATTTGGA TGAAACCAAC GCGGAGGAGC TTGACGAGGA GGATTTCCAG 
GAGGAACAAA ATTCAGTAGC CCGTCTCATA CATATGCTTG ATAATGAAGA ACCAGAGGAG 
ATGCTTAAGA TTATATGTGT TGTGAGACGA CATCTTATGA CAGGAGGACC TCGACGCTTG 
CCTTTTACTG TTCCCCCACT TGTTTTTTCT GCAGTTAGGT TGGTGAGGCA GCTAGAATCC 
CAGGGTGGAG ATATAGCAGG AGAAGATTCA GCAACCCCGA GAAAGATTTT TCAGATTCTC 
AACCAGACAA TCGAGGTTCT TACCTCAGTT CCATGTCCTG AACTGGCTCT AAGGCTCTAC 
CTTCAGTGCG CAGAGGCCGC AAGTGACTGT GATCTTGAGC CTGTTGCTTA TGAATTTTTT 
ACCCAAGCAT TTATGTTATA CGAGGAAGAA ATTGCGGACT CGAAGGCACA GGTAACTGCG 
ATTCATCTGA TCGTTGGAAC CTTGCAAAGG ATCAATGTCT TTGGTGTTGA AAATAGGGAC 
ACCTTGACAC ATAAGGCAAC CGGGTACTCA GCAAGGCTTC TTAAGAAGCC TGACCAATGC 
CGAGCGGTTT ACGCATGCTC TCACCTCTTT TGGGTCGATG ATCCAGATGG TATTAAAGAT 
GGAGAAAGAG TTCTTCTCTG TTTAAGACGT GCACTGAGGA TAGCGAACGC AGCTCAACAA 
ATGGCTAGTG CAACCAGAGG TAGCAGCGGA CCAGTCACGC TGTTTGTTGA AATCTTGAAC 
AAGTACATTT ACTTCTTTGA GAAAGGGAAT CCACATATCA CTCCATCTGA TATACAGAGC 
CTCATAGAAT TGATCAACAA TGAGATGCAA AGCGACAACG GTAACACGAC AATCCACTCA 
GATCCCTTCT TTACAAGTAC ATTGCGTTAC ATTAAGTTCA TAAAACAGAA AGGTGGTTTG 
ATGGGTGAGA AATATGACCC TATCAAGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_177713.3
CDS	73..2445
Translation	MRTLAGVEDEDKWLAEGIAGIQHNAFFMHRALDANNLREVLKYSALMLSELRTSKLSPQKYYDLYMRAFDQLRQLEIFFKDESRHGLPVVDLYELVQHAGNILPRMYLLCTVGSVYIKSKQAPSKDVLKDLVEMCRGVQHPIRGLFLRSYLAQVSRDKLPEIGSDYEGDANTVMDAVEFVLQNFTEMNKLWVRIQHQGPGTVREKQEKERNELRDLVGKNLHVLGQIEGVDLEMYKETVLPRVLEQVVNCKDKLAQYYLMECIIQVFPDEYHLQTLETLLAACTQLMPTVDTKIVLTQLMDRLSNYAASSPDVLHEFLQVEAFAKLSNAIGKVIDTQLEMPIVGAMTLFVSLLTFTLRVHPDRLDYVDQVLGACVVKLSSVPKLEDARAMKQVVALLSAPLEKYSDIVTALTLSNYPRVMDHLDDGTNKVMAMLIIQSIMKTDSCISTADKVEVLFELIKGLIKDLDETNAEELDEEDFQEEQNSVARLIHMLDNEEPEEMLKIICVVRRHLMTGGPRRLPFTVPPLVFSAVRLVRQLESQGGDIAGEDSATPRKIFQILNQTIEVLTSVPCPELALRLYLQCAEAASDCDLEPVAYEFFTQAFMLYEEEIADSKAQVTAIHLIVGTLQRINVFGVENRDTLTHKATGYSARLLKKPDQCRAVYACSHLFWVDDPDGIKDGERVLLCLRRALRIANAAQQMASATRGSSGPVTLFVEILNKYIYFFEKGNPHITPSDIQSLIELINNEMQSDNGNTTIHSDPFFTSTLRYIKFIKQKGGLMGEKYDPIKL

Target ORF information:

RefSeq Version	NM_106235.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana VPS35 homolog B (VPS35B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_106235.5

ORF Insert Sequence:

ATGAGAACGC TCGCCGGAGT AGAAGACGAG GATAAGTGGC TGGCGGAAGG AATCGCCGGA 
ATCCAGCACA ACGCTTTCTT CATGCATCGC GCTTTGGACG CGAATAATCT CAGAGAAGTT 
CTCAAGTACT CTGCTCTGAT GTTATCGGAG CTTCGAACCT CCAAGCTCTC TCCTCAGAAA 
TATTACGATC TCTATATGAG AGCTTTCGAT CAATTGAGGC AGCTGGAGAT TTTCTTTAAG 
GATGAGAGTA GGCATGGATT ACCTGTTGTT GATTTGTATG AACTTGTTCA ACATGCCGGC 
AACATATTGC CCAGAATGTA TCTGCTATGT ACGGTCGGGT CTGTTTATAT CAAAAGTAAG 
CAGGCTCCTT CGAAGGATGT ACTTAAGGAT CTTGTGGAAA TGTGTCGAGG TGTTCAACAT 
CCTATTCGTG GCCTCTTTTT GAGGAGTTAT CTCGCTCAAG TGAGCAGGGA TAAGTTACCT 
GAGATTGGCT CAGACTACGA AGGAGATGCA AATACTGTGA TGGATGCTGT GGAGTTTGTG 
CTACAAAATT TCACAGAGAT GAATAAGCTT TGGGTTCGAA TACAGCATCA GGGACCAGGA 
ACAGTTCGAG AAAAGCAGGA GAAAGAGAGG AACGAACTTC GTGATCTTGT TGGAAAAAAT 
CTGCATGTTC TAGGACAGAT AGAAGGTGTT GACCTTGAGA TGTACAAAGA GACTGTTCTT 
CCTAGGGTCT TAGAGCAGGT CGTCAACTGT AAAGATAAGT TGGCTCAGTA TTATCTGATG 
GAGTGCATAA TTCAAGTCTT TCCTGATGAG TACCATTTGC AGACTCTTGA GACCTTATTG 
GCTGCTTGTA CCCAACTGAT GCCAACTGTT GACACGAAGA TAGTGTTAAC CCAACTAATG 
GACCGGTTAT CAAACTATGC TGCCTCAAGT CCAGATGTGT TACACGAATT TTTGCAAGTG 
GAAGCTTTTG CTAAATTGAG CAATGCAATT GGAAAGGTGA TAGATACACA GCTTGAGATG 
CCTATTGTCG GAGCTATGAC TCTGTTTGTC TCTCTTTTGA CTTTTACTCT CCGAGTTCAT 
CCTGATCGGC TTGACTATGT GGATCAAGTA TTGGGTGCAT GTGTAGTTAA GCTTTCCAGT 
GTGCCCAAGC TGGAGGACGC CCGTGCAATG AAACAAGTAG TGGCACTTTT GAGTGCTCCT 
TTAGAAAAGT ACAGTGACAT AGTTACAGCT CTTACATTGT CAAATTATCC GCGTGTCATG 
GATCATCTTG ATGATGGAAC GAACAAAGTA ATGGCTATGC TAATTATTCA AAGTATAATG 
AAAACTGATT CGTGTATATC AACTGCTGAT AAGGTTGAGG TGCTGTTTGA ATTGATAAAA 
GGACTTATTA AAGATTTGGA TGAAACCAAC GCGGAGGAGC TTGACGAGGA GGATTTCCAG 
GAGGAACAAA ATTCAGTAGC CCGTCTCATA CATATGCTTG ATAATGAAGA ACCAGAGGAG 
ATGCTTAAGA TTATATGTGT TGTGAGACGA CATCTTATGA CAGGAGGACC TCGACGCTTG 
CCTTTTACTG TTCCCCCACT TGTTTTTTCT GCAGTTAGGT TGGTGAGGCA GCTAGAATCC 
CAGGGTGGAG ATATAGCAGG AGAAGATTCA GCAACCCCGA GAAAGATTTT TCAGATTCTC 
AACCAGACAA TCGAGGTTCT TACCTCAGTT CCATGTCCTG AACTGGCTCT AAGGCTCTAC 
CTTCAGTGCG CAGAGGCCGC AAGTGACTGT GATCTTGAGC CTGTTGCTTA TGAATTTTTT 
ACCCAAGCAT TTATGTTATA CGAGGAAGAA ATTGCGGACT CGAAGGCACA GGTAACTGCG 
ATTCATCTGA TCGTTGGAAC CTTGCAAAGG ATCAATGTCT TTGGTGTTGA AAATAGGGAC 
ACCTTGACAC ATAAGGCAAC CGGGTACTCA GCAAGGCTTC TTAAGAAGCC TGACCAATGC 
CGAGCGGTTT ACGCATGCTC TCACCTCTTT TGGGTCGATG ATCCAGATGG TATTAAAGAT 
GGAGAAAGAG TTCTTCTCTG TTTAAGACGT GCACTGAGGA TAGCGAACGC AGCTCAACAA 
ATGGCTAGTG CAACCAGAGG TAGCAGCGGA CCAGTCACGC TGTTTGTTGA AATCTTGAAC 
AAGTACATTT ACTTCTTTGA GAAAGGGAAT CCACATATCA CTCCATCTGA TATACAGAGC 
CTCATAGAAT TGATCAACAA TGAGATGCAA AGCGACAACG GTAACACGAC AATCCACTCA 
GATCCCTTCT TTACAAGTAC ATTGCGTTAC ATTAAGTTCA TAAAACAGAA AGGTGGTTTG 
ATGGGTGAGA AATATGACCC TATCAAGCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS35B ( NM_001334699.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001321274.1 Arabidopsis thaliana VPS35 homolog B (VPS35B), mRNA.

Price & Availability↑

CloneID	OAb34820
Clone ID Related Accession (Same CDS sequence)	NM_001334699.1
Accession Version	NM_001334699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	VPS35 homolog B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGTACAAAG AGACTGTTCT TCCTAGGGTC TTAGAGCAGG TCGTCAACTG TAAAGATAAG 
TTGGCTCAGT ATTATCTGAT GGAGTGCATA ATTCAAGTCT TTCCTGATGA GTACCATTTG 
CAGACTCTTG AGACCTTATT GGCTGCTTGT ACCCAACTGA TGCCAACTGT TGACACGAAG 
ATAGTGTTAA CCCAACTAAT GGACCGGTTA TCAAACTATG CTGCCTCAAG TCCAGATGTG 
TTACACGAAT TTTTGCAAGT GGAAGCTTTT GCTAAATTGA GCAATGCAAT TGGAAAGGTG 
ATAGATACAC AGCTTGAGAT GCCTATTGTC GGAGCTATGA CTCTGTTTGT CTCTCTTTTG 
ACTTTTACTC TCCGAGTTCA TCCTGATCGG CTTGACTATG TGGATCAAGT ATTGGGTGCA 
TGTGTAGTTA AGCTTTCCAG TGTGCCCAAG CTGGAGGACG CCCGTGCAAT GAAACAAGTA 
GTGGCACTTT TGAGTGCTCC TTTAGAAAAG TACAGTGACA TAGTTACAGC TCTTACATTG 
TCAAATTATC CGCGTGTCAT GGATCATCTT GATGATGGAA CGAACAAAGT AATGGCTATG 
CTAATTATTC AAAGTATAAT GAAAACTGAT TCGTGTATAT CAACTGCTGA TAAGGTTGAG 
GTGCTGTTTG AATTGATAAA AGGACTTATT AAAGATTTGG ATGAAACCAA CGCGGAGGAG 
CTTGACGAGG AGGATTTCCA GGAGGAACAA AATTCAGTAG CCCGTCTCAT ACATATGCTT 
GATAATGAAG AACCAGAGGA GATGCTTAAG ATTATATGTG TTGTGAGACG ACATCTTATG 
ACAGGAGGAC CTCGACGCTT GCCTTTTACT GTTCCCCCAC TTGTTTTTTC TGCAGTTAGG 
TTGGTGAGGC AGCTAGAATC CCAGGGTGGA GATATAGCAG GAGAAGATTC AGCAACCCCG 
AGAAAGATTT TTCAGATTCT CAACCAGACA ATCGAGGTTC TTACCTCAGT TCCATGTCCT 
GAACTGGCTC TAAGGCTCTA CCTTCAGTGC GCAGAGGCCG CAAGTGACTG TGATCTTGAG 
CCTGTTGCTT ATGAATTTTT TACCCAAGCA TTTATGTTAT ACGAGGAAGA AATTGCGGAC 
TCGAAGGCAC AGGTAACTGC GATTCATCTG ATCGTTGGAA CCTTGCAAAG GATCAATGTC 
TTTGGTGTTG AAAATAGGGA CACCTTGACA CATAAGGCAA CCGGGTACTC AGCAAGGCTT 
CTTAAGAAGC CTGACCAATG CCGAGCGGTT TACGCATGCT CTCACCTCTT TTGGGTCGAT 
GATCCAGATG GTATTAAAGA TGGAGAAAGA GTTCTTCTCT GTTTAAGACG TGCACTGAGG 
ATAGCGAACG CAGCTCAACA AATGGCTAGT GCAACCAGAG GTAGCAGCGG ACCAGTCACG 
CTGTTTGTTG AAATCTTGAA CAAGTACATT TACTTCTTTG AGAAAGGGAA TCCACATATC 
ACTCCATCTG ATATACAGAG CCTCATAGAA TTGATCAACA ATGAGATGCA AAGCGACAAC 
GGTAACACGA CAATCCACTC AGATCCCTTC TTTACAAGTA CATTGCGTTA CATTAAGTTC 
ATAAAACAGA AAGGTGGTTT GATGGGTGAG AAATATGACC CTATCAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001321274.1
CDS	147..1820
Translation	MYKETVLPRVLEQVVNCKDKLAQYYLMECIIQVFPDEYHLQTLETLLAACTQLMPTVDTKIVLTQLMDRLSNYAASSPDVLHEFLQVEAFAKLSNAIGKVIDTQLEMPIVGAMTLFVSLLTFTLRVHPDRLDYVDQVLGACVVKLSSVPKLEDARAMKQVVALLSAPLEKYSDIVTALTLSNYPRVMDHLDDGTNKVMAMLIIQSIMKTDSCISTADKVEVLFELIKGLIKDLDETNAEELDEEDFQEEQNSVARLIHMLDNEEPEEMLKIICVVRRHLMTGGPRRLPFTVPPLVFSAVRLVRQLESQGGDIAGEDSATPRKIFQILNQTIEVLTSVPCPELALRLYLQCAEAASDCDLEPVAYEFFTQAFMLYEEEIADSKAQVTAIHLIVGTLQRINVFGVENRDTLTHKATGYSARLLKKPDQCRAVYACSHLFWVDDPDGIKDGERVLLCLRRALRIANAAQQMASATRGSSGPVTLFVEILNKYIYFFEKGNPHITPSDIQSLIELINNEMQSDNGNTTIHSDPFFTSTLRYIKFIKQKGGLMGEKYDPIKL

Target ORF information:

RefSeq Version	NM_001334699.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana VPS35 homolog B (VPS35B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001334699.1

ORF Insert Sequence:

ATGTACAAAG AGACTGTTCT TCCTAGGGTC TTAGAGCAGG TCGTCAACTG TAAAGATAAG 
TTGGCTCAGT ATTATCTGAT GGAGTGCATA ATTCAAGTCT TTCCTGATGA GTACCATTTG 
CAGACTCTTG AGACCTTATT GGCTGCTTGT ACCCAACTGA TGCCAACTGT TGACACGAAG 
ATAGTGTTAA CCCAACTAAT GGACCGGTTA TCAAACTATG CTGCCTCAAG TCCAGATGTG 
TTACACGAAT TTTTGCAAGT GGAAGCTTTT GCTAAATTGA GCAATGCAAT TGGAAAGGTG 
ATAGATACAC AGCTTGAGAT GCCTATTGTC GGAGCTATGA CTCTGTTTGT CTCTCTTTTG 
ACTTTTACTC TCCGAGTTCA TCCTGATCGG CTTGACTATG TGGATCAAGT ATTGGGTGCA 
TGTGTAGTTA AGCTTTCCAG TGTGCCCAAG CTGGAGGACG CCCGTGCAAT GAAACAAGTA 
GTGGCACTTT TGAGTGCTCC TTTAGAAAAG TACAGTGACA TAGTTACAGC TCTTACATTG 
TCAAATTATC CGCGTGTCAT GGATCATCTT GATGATGGAA CGAACAAAGT AATGGCTATG 
CTAATTATTC AAAGTATAAT GAAAACTGAT TCGTGTATAT CAACTGCTGA TAAGGTTGAG 
GTGCTGTTTG AATTGATAAA AGGACTTATT AAAGATTTGG ATGAAACCAA CGCGGAGGAG 
CTTGACGAGG AGGATTTCCA GGAGGAACAA AATTCAGTAG CCCGTCTCAT ACATATGCTT 
GATAATGAAG AACCAGAGGA GATGCTTAAG ATTATATGTG TTGTGAGACG ACATCTTATG 
ACAGGAGGAC CTCGACGCTT GCCTTTTACT GTTCCCCCAC TTGTTTTTTC TGCAGTTAGG 
TTGGTGAGGC AGCTAGAATC CCAGGGTGGA GATATAGCAG GAGAAGATTC AGCAACCCCG 
AGAAAGATTT TTCAGATTCT CAACCAGACA ATCGAGGTTC TTACCTCAGT TCCATGTCCT 
GAACTGGCTC TAAGGCTCTA CCTTCAGTGC GCAGAGGCCG CAAGTGACTG TGATCTTGAG 
CCTGTTGCTT ATGAATTTTT TACCCAAGCA TTTATGTTAT ACGAGGAAGA AATTGCGGAC 
TCGAAGGCAC AGGTAACTGC GATTCATCTG ATCGTTGGAA CCTTGCAAAG GATCAATGTC 
TTTGGTGTTG AAAATAGGGA CACCTTGACA CATAAGGCAA CCGGGTACTC AGCAAGGCTT 
CTTAAGAAGC CTGACCAATG CCGAGCGGTT TACGCATGCT CTCACCTCTT TTGGGTCGAT 
GATCCAGATG GTATTAAAGA TGGAGAAAGA GTTCTTCTCT GTTTAAGACG TGCACTGAGG 
ATAGCGAACG CAGCTCAACA AATGGCTAGT GCAACCAGAG GTAGCAGCGG ACCAGTCACG 
CTGTTTGTTG AAATCTTGAA CAAGTACATT TACTTCTTTG AGAAAGGGAA TCCACATATC 
ACTCCATCTG ATATACAGAG CCTCATAGAA TTGATCAACA ATGAGATGCA AAGCGACAAC 
GGTAACACGA CAATCCACTC AGATCCCTTC TTTACAAGTA CATTGCGTTA CATTAAGTTC 
ATAAAACAGA AAGGTGGTTT GATGGGTGAG AAATATGACC CTATCAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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VPS35B cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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