HSP101 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSP101
Entrez Gene ID	843771
Full Name	heat shock protein 101
Synonyms	ATHSP101,F1O17.2,F1O17_2,HEAT SHOCK PROTEIN ATHSP101,HOT1,heat shock protein 101
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_106091.4	NP_565083.1	heat shock protein 101
NM_001334640.1	NP_001320516.1	heat shock protein 101

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGL036C	NP_986630.1
Arabidopsis thaliana (thale cress)	HSP101	NP_565083.1
Saccharomyces cerevisiae (baker's yeast)	HSP104	NP_013074.1
Neurospora crassa	NCU00104	XP_957228.1
Oryza sativa (rice)	Os05g0519700	NP_001056066.1
Schizosaccharomyces pombe (fission yeast)	SPBC16D10.08c	NP_596503.1
Magnaporthe oryzae (rice blast fungus)	MGG_06459	XP_003717107.1
Kluyveromyces lactis	KLLA0F18194g	XP_455896.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

after heat shock, HSP101 is required for the efficient release of RP mRNAs from stress granules resulting in a rapid restoration of the translation machinery by producing new ribosomal proteins.
Title: Heat Shock Protein HSP101 Affects the Release of Ribosomal Protein mRNAs for Recovery after Heat Shock.

Data show that both class I and II small heat shock proteins (sHSPs) were present in cytosolic foci, some of which colocalized with HSP101 and with eEF1Bgamma and eEF1Bbeta.
Title: Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress.

a positive feedback loop between HSP101 and HSA32 at the protein level is a novel mechanism for prolonging the memory of heat acclimation.
Title: Interplay between heat shock proteins HSP101 and HSA32 prolongs heat acclimation memory posttranscriptionally in Arabidopsis.

This study shows that Hsp101 expression is evolvable in natural populations.Hsp101 is primarily an emergency high-temperature tolerance mechanism, since expression levels are lower in low-latitude populations from warmer climates.
Title: Heat shock protein 101 effects in A. thaliana: genetic variation, fitness and pleiotropy in controlled temperature conditions.

The following HSP101 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSP101 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb27937	NM_106091.4 Latest version!	Arabidopsis thaliana heat shock protein 101 (HSP101), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb34619	NM_001334640.1 Latest version!	Arabidopsis thaliana heat shock protein 101 (HSP101), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSP101 ( NM_106091.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_565083.1 Arabidopsis thaliana heat shock protein 101 (HSP101), mRNA.

Price & Availability↑

CloneID	OAb27937
Clone ID Related Accession (Same CDS sequence)	NM_106091.4
Accession Version	NM_106091.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heat shock protein 101
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_106091.3.

ATGAATCCAG AGAAATTCAC ACACAAGACA AACGAGACAA TTGCTACAGC TCATGAGCTA 
GCTGTGAATG CAGGACATGC TCAATTCACT CCTTTGCATT TAGCTGGTGC TTTGATCTCT 
GATCCCACCG GTATATTTCC TCAAGCAATC TCTAGTGCCG GTGGCGAGAA CGCAGCTCAA 
TCTGCTGAAA GAGTGATCAA TCAAGCCTTG AAGAAGCTTC CTTCACAATC TCCTCCACCT 
GATGATATTC CAGCGAGTTC TAGTCTTATT AAGGTCATTC GTCGTGCTCA AGCTGCTCAG 
AAGTCACGAG GTGATACTCA TTTGGCTGTT GACCAGTTGA TTATGGGTCT TCTTGAAGAT 
TCTCAAATCA GGGATTTGTT GAACGAAGTC GGTGTAGCGA CGGCGAGGGT AAAGTCTGAG 
GTTGAGAAGC TTCGTGGGAA AGAAGGGAAG AAAGTTGAGA GTGCTTCAGG GGACACAAAT 
TTTCAAGCTT TAAAGACTTA TGGAAGAGAT TTGGTTGAGC AAGCAGGGAA GCTTGATCCT 
GTGATTGGTC GTGATGAGGA GATTAGAAGA GTCGTGAGGA TTCTTTCGAG GAGAACGAAG 
AACAATCCTG TGCTTATTGG AGAGCCAGGA GTTGGTAAAA CAGCTGTGGT TGAAGGTTTA 
GCACAAAGGA TTGTGAAAGG AGATGTGCCC AACAGTCTTA CTGATGTGAG ATTAATTTCG 
TTGGACATGG GTGCGTTAGT TGCTGGTGCT AAATACCGAG GAGAGTTTGA AGAAAGGTTG 
AAATCTGTTT TGAAAGAAGT TGAGGACGCT GAAGGCAAAG TGATTCTCTT TATTGATGAG 
ATTCATTTGG TTCTTGGTGC TGGCAAAACT GAAGGGTCGA TGGATGCAGC TAATCTGTTC 
AAGCCCATGT TAGCTAGAGG GCAGCTTCGA TGCATTGGTG CTACAACGCT TGAAGAATAC 
AGGAAATATG TTGAGAAAGA TGCTGCCTTT GAGAGGAGGT TCCAACAAGT CTATGTTGCG 
GAGCCAAGTG TGCCTGACAC CATTAGTATC CTTAGAGGAC TCAAGGAGAA GTATGAGGGA 
CATCATGGTG TGCGAATCCA AGACAGAGCT CTTATAAATG CTGCTCAGCT GTCTGCTCGT 
TACATAACTG GTCGGCATTT ACCGGATAAA GCAATTGATT TGGTTGATGA GGCTTGTGCG 
AATGTGAGAG TCCAGCTTGA TAGTCAACCT GAAGAGATTG ATAACCTTGA AAGGAAGAGG 
ATGCAGCTGG AAATTGAACT TCACGCCTTG GAAAGGGAGA AGGATAAAGC CAGCAAAGCT 
CGACTTATAG AGGTGCGGAA AGAGCTTGAT GACCTGAGAG ACAAGCTTCA GCCTCTCACG 
ATGAAATACA GAAAGGAGAA AGAGAGAATT GATGAGATTC GAAGGCTTAA ACAGAAAAGA 
GAAGAGCTCA TGTTTTCTTT GCAGGAGGCA GAACGAAGAT ATGACCTTGC AAGAGCTGCT 
GATCTAAGAT ATGGCGCAAT TCAAGAAGTG GAATCTGCAA TTGCCCAACT TGAAGGAACT 
TCTTCTGAAG AGAATGTGAT GCTCACAGAA AACGTTGGGC CTGAACACAT TGCTGAGGTT 
GTGAGCCGTT GGACAGGGAT TCCAGTGACG AGACTTGGCC AAAATGAGAA GGAGAGGTTG 
ATTGGTCTTG CTGATAGGTT GCATAAGCGG GTTGTGGGAC AGAATCAAGC GGTAAATGCA 
GTTTCTGAGG CAATTCTAAG GTCAAGGGCA GGACTTGGAA GGCCACAACA GCCAACTGGA 
TCATTCTTAT TCCTTGGACC AACTGGTGTT GGCAAAACTG AGCTCGCCAA GGCTCTTGCT 
GAGCAGCTGT TTGATGATGA AAACCTCTTA GTTCGGATTG ATATGTCGGA ATATATGGAA 
CAACACTCTG TCTCTCGCCT CATTGGGGCA CCACCAGGGT ATGTTGGTCA CGAGGAAGGT 
GGACAACTAA CTGAGGCTGT GAGGAGGCGA CCTTATTGTG TCATACTCTT TGATGAAGTG 
GAGAAGGCTC ATGTTGCTGT CTTCAACACT CTGCTCCAAG TTTTGGATGA TGGTCGATTG 
ACAGACGGGC AAGGCAGGAC AGTCGATTTC AGGAACTCGG TGATAATCAT GACATCAAAC 
CTTGGTGCTG AACACCTCCT TGCAGGGCTA ACTGGGAAAG TAACAATGGA AGTGGCCCGG 
GACTGTGTGA TGCGGGAGGT GAGGAAACAC TTCAGACCAG AGCTCTTGAA CAGGCTTGAC 
GAGATTGTGG TGTTCGACCC CCTTTCACAT GACCAGTTGA GGAAAGTAGC TCGGCTTCAA 
ATGAAAGACG TTGCTGTCCG GCTTGCTGAA AGAGGAGTTG CTTTGGCAGT CACTGATGCT 
GCTTTGGACT ATATCTTGGC AGAGAGTTAT GACCCGGTGT ATGGTGCTAG GCCTATAAGG 
AGATGGATGG AGAAGAAGGT GGTGACAGAA CTGTCAAAGA TGGTTGTGCG TGAGGAAATC 
GATGAAAACT CCACTGTTTA CATAGATGCA GGCGCTGGTG ATCTTGTGTA CCGGGTAGAA 
AGTGGAGGTC TAGTGGACGC TTCAACAGGC AAGAAGTCAG ATGTGCTGAT TCATATTGCT 
AACGGGCCAA AGAGAAGTGA TGCAGCTCAG GCGGTGAAGA AGATGAGGAT CGAGGAAATA 
GAAGATGACG ATAATGAGGA AATGATCGAG GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_565083.1
CDS	342..3077
Translation	MNPEKFTHKTNETIATAHELAVNAGHAQFTPLHLAGALISDPTGIFPQAISSAGGENAAQSAERVINQALKKLPSQSPPPDDIPASSSLIKVIRRAQAAQKSRGDTHLAVDQLIMGLLEDSQIRDLLNEVGVATARVKSEVEKLRGKEGKKVESASGDTNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVKGDVPNSLTDVRLISLDMGALVAGAKYRGEFEERLKSVLKEVEDAEGKVILFIDEIHLVLGAGKTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFERRFQQVYVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALINAAQLSARYITGRHLPDKAIDLVDEACANVRVQLDSQPEEIDNLERKRMQLEIELHALEREKDKASKARLIEVRKELDDLRDKLQPLTMKYRKEKERIDEIRRLKQKREELMFSLQEAERRYDLARAADLRYGAIQEVESAIAQLEGTSSEENVMLTENVGPEHIAEVVSRWTGIPVTRLGQNEKERLIGLADRLHKRVVGQNQAVNAVSEAILRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQLFDDENLLVRIDMSEYMEQHSVSRLIGAPPGYVGHEEGGQLTEAVRRRPYCVILFDEVEKAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNSVIIMTSNLGAEHLLAGLTGKVTMEVARDCVMREVRKHFRPELLNRLDEIVVFDPLSHDQLRKVARLQMKDVAVRLAERGVALAVTDAALDYILAESYDPVYGARPIRRWMEKKVVTELSKMVVREEIDENSTVYIDAGAGDLVYRVESGGLVDASTGKKSDVLIHIANGPKRSDAAQAVKKMRIEEIEDDDNEEMIED

Target ORF information:

RefSeq Version	NM_106091.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heat shock protein 101 (HSP101), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_106091.4

ORF Insert Sequence:

ATGAATCCAG AGAAATTCAC ACACAAGACA AACGAGACAA TTGCTACAGC TCATGAGCTA 
GCTGTGAATG CAGGACATGC TCAATTCACT CCTTTGCATT TAGCTGGTGC TTTGATCTCT 
GATCCCACCG GTATATTTCC TCAAGCAATC TCTAGTGCCG GTGGCGAGAA CGCAGCTCAA 
TCTGCTGAAA GAGTGATCAA TCAAGCCTTG AAGAAGCTTC CTTCACAATC TCCTCCACCT 
GATGATATTC CAGCGAGTTC TAGTCTTATT AAGGTCATTC GTCGTGCTCA AGCTGCTCAG 
AAGTCACGAG GTGATACTCA TTTGGCTGTT GACCAGTTGA TTATGGGTCT TCTTGAAGAT 
TCTCAAATCA GGGATTTGTT GAACGAAGTC GGTGTAGCGA CGGCGAGGGT AAAGTCTGAG 
GTTGAGAAGC TTCGTGGGAA AGAAGGGAAG AAAGTTGAGA GTGCTTCAGG GGACACAAAT 
TTTCAAGCTT TAAAGACTTA TGGAAGAGAT TTGGTTGAGC AAGCAGGGAA GCTTGATCCT 
GTGATTGGTC GTGATGAGGA GATTAGAAGA GTCGTGAGGA TTCTTTCGAG GAGAACGAAG 
AACAATCCTG TGCTTATTGG AGAGCCAGGA GTTGGTAAAA CAGCTGTGGT TGAAGGTTTA 
GCACAAAGGA TTGTGAAAGG AGATGTGCCC AACAGTCTTA CTGATGTGAG ATTAATTTCG 
TTGGACATGG GTGCGTTAGT TGCTGGTGCT AAATACCGAG GAGAGTTTGA AGAAAGGTTG 
AAATCTGTTT TGAAAGAAGT TGAGGACGCT GAAGGCAAAG TGATTCTCTT TATTGATGAG 
ATTCATTTGG TTCTTGGTGC TGGCAAAACT GAAGGGTCGA TGGATGCAGC TAATCTGTTC 
AAGCCCATGT TAGCTAGAGG GCAGCTTCGA TGCATTGGTG CTACAACGCT TGAAGAATAC 
AGGAAATATG TTGAGAAAGA TGCTGCCTTT GAGAGGAGGT TCCAACAAGT CTATGTTGCG 
GAGCCAAGTG TGCCTGACAC CATTAGTATC CTTAGAGGAC TCAAGGAGAA GTATGAGGGA 
CATCATGGTG TGCGAATCCA AGACAGAGCT CTTATAAATG CTGCTCAGCT GTCTGCTCGT 
TACATAACTG GTCGGCATTT ACCGGATAAA GCAATTGATT TGGTTGATGA GGCTTGTGCG 
AATGTGAGAG TCCAGCTTGA TAGTCAACCT GAAGAGATTG ATAACCTTGA AAGGAAGAGG 
ATGCAGCTGG AAATTGAACT TCACGCCTTG GAAAGGGAGA AGGATAAAGC CAGCAAAGCT 
CGACTTATAG AGGTGCGGAA AGAGCTTGAT GACCTGAGAG ACAAGCTTCA GCCTCTCACG 
ATGAAATACA GAAAGGAGAA AGAGAGAATT GATGAGATTC GAAGGCTTAA ACAGAAAAGA 
GAAGAGCTCA TGTTTTCTTT GCAGGAGGCA GAACGAAGAT ATGACCTTGC AAGAGCTGCT 
GATCTAAGAT ATGGCGCAAT TCAAGAAGTG GAATCTGCAA TTGCCCAACT TGAAGGAACT 
TCTTCTGAAG AGAATGTGAT GCTCACAGAA AACGTTGGGC CTGAACACAT TGCTGAGGTT 
GTGAGCCGTT GGACAGGGAT TCCAGTGACG AGACTTGGCC AAAATGAGAA GGAGAGGTTG 
ATTGGTCTTG CTGATAGGTT GCATAAGCGG GTTGTGGGAC AGAATCAAGC GGTAAATGCA 
GTTTCTGAGG CAATTCTAAG GTCAAGGGCA GGACTTGGAA GGCCACAACA GCCAACTGGA 
TCATTCTTAT TCCTTGGACC AACTGGTGTT GGCAAAACTG AGCTCGCCAA GGCTCTTGCT 
GAGCAGCTGT TTGATGATGA AAACCTCTTA GTTCGGATTG ATATGTCGGA ATATATGGAA 
CAACACTCTG TCTCTCGCCT CATTGGGGCA CCACCAGGGT ATGTTGGTCA CGAGGAAGGT 
GGACAACTAA CTGAGGCTGT GAGGAGGCGA CCTTATTGTG TCATACTCTT TGATGAAGTG 
GAGAAGGCTC ATGTTGCTGT CTTCAACACT CTGCTCCAAG TTTTGGATGA TGGTCGATTG 
ACAGACGGGC AAGGCAGGAC AGTCGATTTC AGGAACTCGG TGATAATCAT GACATCAAAC 
CTTGGTGCTG AACACCTCCT TGCAGGGCTA ACTGGGAAAG TAACAATGGA AGTGGCCCGG 
GACTGTGTGA TGCGGGAGGT GAGGAAACAC TTCAGACCAG AGCTCTTGAA CAGGCTTGAC 
GAGATTGTGG TGTTCGACCC CCTTTCACAT GACCAGTTGA GGAAAGTAGC TCGGCTTCAA 
ATGAAAGACG TTGCTGTCCG GCTTGCTGAA AGAGGAGTTG CTTTGGCAGT CACTGATGCT 
GCTTTGGACT ATATCTTGGC AGAGAGTTAT GACCCGGTGT ATGGTGCTAG GCCTATAAGG 
AGATGGATGG AGAAGAAGGT GGTGACAGAA CTGTCAAAGA TGGTTGTGCG TGAGGAAATC 
GATGAAAACT CCACTGTTTA CATAGATGCA GGCGCTGGTG ATCTTGTGTA CCGGGTAGAA 
AGTGGAGGTC TAGTGGACGC TTCAACAGGC AAGAAGTCAG ATGTGCTGAT TCATATTGCT 
AACGGGCCAA AGAGAAGTGA TGCAGCTCAG GCGGTGAAGA AGATGAGGAT CGAGGAAATA 
GAAGATGACG ATAATGAGGA AATGATCGAG GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSP101 ( NM_001334640.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001320516.1 Arabidopsis thaliana heat shock protein 101 (HSP101), partial mRNA.

Price & Availability↑

CloneID	OAb34619
Clone ID Related Accession (Same CDS sequence)	NM_001334640.1
Accession Version	NM_001334640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heat shock protein 101
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). COMPLETENESS: incomplete on the 3' end.

ATGAATCCAG AGAAATTCAC ACACAAGACA AACGAGACAA TTGCTACAGC TCATGAGCTA 
GCTGTGAATG CAGGACATGC TCAATTCACT CCTTTGCATT TAGCTGGTGC TTTGATCTCT 
GATCCCACCG GTATATTTCC TCAAGCAATC TCTAGTGCCG GTGGCGAGAA CGCAGCTCAA 
TCTGCTGAAA GAGTGATCAA TCAAGCCTTG AAGAAGCTTC CTTCACAATC TCCTCCACCT 
GATGATATTC CAGCGAGTTC TAGTCTTATT AAGGTCATTC GTCGTGCTCA AGCTGCTCAG 
AAGTCACGAG GTGATACTCA TTTGGCTGTT GACCAGTTGA TTATGGGTCT TCTTGAAGAT 
TCTCAAATCA GGGATTTGTT GAACGAAGTC GGTGTAGCGA CGGCGAGGGT AAAGTCTGAG 
GTTGAGAAGC TTCGTGGGAA AGAAGGGAAG AAAGTTGAGA GTGCTTCAGG GGACACAAAT 
TTTCAAGCTT TAAAGACTTA TGGAAGAGAT TTGGTTGAGC AAGCAGGGAA GCTTGATCCT 
GTGATTGGTC GTGATGAGGA GATTAGAAGA GTCGTGAGGA TTCTTTCGAG GAGAACGAAG 
AACAATCCTG TGCTTATTGG AGAGCCAGGA GTTGGTAAAA CAGCTGTGGT TGAAGGTTTA 
GCACAAAGGA TTGTGAAAGG AGATGTGCCC AACAGTCTTA CTGATGTGAG ATTAATTTCG 
TTGGACATGG GTGCGTTAGT TGCTGGTGCT AAATACCGAG GAGAGTTTGA AGAAAGGTTG 
AAATCTGTTT TGAAAGAAGT TGAGGACGCT GAAGGCAAAG TGATTCTCTT TATTGATGAG 
ATTCATTTGG TTCTTGGTGC TGGCAAAACT GAAGGGTCGA TGGATGCAGC TAATCTGTTC 
AAGCCCATGT TAGCTAGAGG GCAGCTTCGA TGCATTGGTG CTACAACGCT TGAAGAATAC 
AGGAAATATG TTGAGAAAGA TGCTGCCTTT GAGAGGAGGT TCCAACAAGT CTATGTTGCG 
GAGCCAAGTG TGCCTGACAC CATTAGTATC CTTAGAGGAC TCAAGGAGAA GTATGAGGGA 
CATCATGGTG TGCGAATCCA AGACAGAGCT CTTATAAATG CTGCTCAGCT GTCTGCTCGT 
TACATAACTG GTCGGCATTT ACCGGATAAA GCAATTGATT TGGTTGATGA GGCTTGTGCG 
AATGTGAGAG TCCAGCTTGA TAGTCAACCT GAAGAGATTG ATAACCTTGA AAGGAAGAGG 
ATGCAGCTGG AAATTGAACT TCACGCCTTG GAAAGGGAGA AGGATAAAGC CAGCAAAGCT 
CGACTTATAG AGGTGCGGAA AGAGCTTGAT GACCTGAGAG ACAAGCTTCA GCCTCTCACG 
ATGAAATACA GAAAGGAGAA AGAGAGAATT GATGAGATTC GAAGGCTTAA ACAGAAAAGA 
GAAGAGCTCA TGTTTTCTTT GCAGGAGGCA GAACGAAGAT ATGACCTTGC AAGAGCTGCT 
GATCTAAGAT ATGGCGCAAT TCAAGAAGTG GAATCTGCAA TTGCCCAACT TGAAGGAACT 
TCTTCTGAAG AGAATGTGAT GCTCACAGAA AACGTTGGGC CTGAACACAT TGCTGAGGTT 
GTGAGCCGTT GGACAGGGAT TCCAGTGACG AGACTTGGCC AAAATGAGAA GGAGAGGTTG 
ATTGGTCTTG CTGATAGGTT GCATAAGCGG GTTGTGGGAC AGAATCAAGC GGTAAATGCA 
GTTTCTGAGG CAATTCTAAG GTCAAGGGCA GGACTTGGAA GGCCACAACA GCCAACTGGA 
TCATTCTTAT TCCTTGGACC AACTGGTGTT GGCAAAACTG AGCTCGCCAA GGCTCTTGCT 
GAGCAGCTGT TTGATGATGA AAACCTCTTA GTTCGGATTG ATATGTCGGA ATATATGGAA 
CAACACTCTG TCTCTCGCCT CATTGGGGCA CCACCAGGGT ATGTTGGTCA CGAGGAAGGT 
GGACAACTAA CTGAGGCTGT GAGGAGGCGA CCTTATTGTG TCATACTCTT TGATGAAGTG 
GAGAAGGCTC ATGTTGCTGT CTTCAACACT CTGCTCCAAG TTTTGGATGA TGGTCGATTG 
ACAGACGGGC AAGGCAGGAC AGTCGATTTC AGGAACTCGG TGATAATCAT GACATCAAAC 
CTTGGTGCTG AACACCTCCT TGCAGGGCTA ACTGGGAAAG TAACAATGGA AGTGGCCCGG 
GACTGTGTGA TGCGGGAGGT GAGGAAACAC TTCAGACCAG AGCTCTTGAA CAGGCTTGAC 
GAGATTGTGG TGTTCGACCC CCTTTCACAT GACCAGTTGA GGAAAGTAGC TCGGCTTCAA 
ATGAAAGACG TTGCTGTCCG GCTTGCTGAA AGAGGAGTTG CTTTGGCAGT CACTGATGCT 
GCTTTGGACT ATATCTTGGC AGAGAGTTAT GACCCGGTAA GTCTCTTCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001320516.1
CDS	402..2855
Translation	MNPEKFTHKTNETIATAHELAVNAGHAQFTPLHLAGALISDPTGIFPQAISSAGGENAAQSAERVINQALKKLPSQSPPPDDIPASSSLIKVIRRAQAAQKSRGDTHLAVDQLIMGLLEDSQIRDLLNEVGVATARVKSEVEKLRGKEGKKVESASGDTNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVKGDVPNSLTDVRLISLDMGALVAGAKYRGEFEERLKSVLKEVEDAEGKVILFIDEIHLVLGAGKTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFERRFQQVYVAEPSVPDTISILRGLKEKYEGHHGVRIQDRALINAAQLSARYITGRHLPDKAIDLVDEACANVRVQLDSQPEEIDNLERKRMQLEIELHALEREKDKASKARLIEVRKELDDLRDKLQPLTMKYRKEKERIDEIRRLKQKREELMFSLQEAERRYDLARAADLRYGAIQEVESAIAQLEGTSSEENVMLTENVGPEHIAEVVSRWTGIPVTRLGQNEKERLIGLADRLHKRVVGQNQAVNAVSEAILRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQLFDDENLLVRIDMSEYMEQHSVSRLIGAPPGYVGHEEGGQLTEAVRRRPYCVILFDEVEKAHVAVFNTLLQVLDDGRLTDGQGRTVDFRNSVIIMTSNLGAEHLLAGLTGKVTMEVARDCVMREVRKHFRPELLNRLDEIVVFDPLSHDQLRKVARLQMKDVAVRLAERGVALAVTDAALDYILAESYDPVSLFT

Target ORF information:

RefSeq Version	NM_001334640.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heat shock protein 101 (HSP101), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001334640.1

ORF Insert Sequence:

ATGAATCCAG AGAAATTCAC ACACAAGACA AACGAGACAA TTGCTACAGC TCATGAGCTA 
GCTGTGAATG CAGGACATGC TCAATTCACT CCTTTGCATT TAGCTGGTGC TTTGATCTCT 
GATCCCACCG GTATATTTCC TCAAGCAATC TCTAGTGCCG GTGGCGAGAA CGCAGCTCAA 
TCTGCTGAAA GAGTGATCAA TCAAGCCTTG AAGAAGCTTC CTTCACAATC TCCTCCACCT 
GATGATATTC CAGCGAGTTC TAGTCTTATT AAGGTCATTC GTCGTGCTCA AGCTGCTCAG 
AAGTCACGAG GTGATACTCA TTTGGCTGTT GACCAGTTGA TTATGGGTCT TCTTGAAGAT 
TCTCAAATCA GGGATTTGTT GAACGAAGTC GGTGTAGCGA CGGCGAGGGT AAAGTCTGAG 
GTTGAGAAGC TTCGTGGGAA AGAAGGGAAG AAAGTTGAGA GTGCTTCAGG GGACACAAAT 
TTTCAAGCTT TAAAGACTTA TGGAAGAGAT TTGGTTGAGC AAGCAGGGAA GCTTGATCCT 
GTGATTGGTC GTGATGAGGA GATTAGAAGA GTCGTGAGGA TTCTTTCGAG GAGAACGAAG 
AACAATCCTG TGCTTATTGG AGAGCCAGGA GTTGGTAAAA CAGCTGTGGT TGAAGGTTTA 
GCACAAAGGA TTGTGAAAGG AGATGTGCCC AACAGTCTTA CTGATGTGAG ATTAATTTCG 
TTGGACATGG GTGCGTTAGT TGCTGGTGCT AAATACCGAG GAGAGTTTGA AGAAAGGTTG 
AAATCTGTTT TGAAAGAAGT TGAGGACGCT GAAGGCAAAG TGATTCTCTT TATTGATGAG 
ATTCATTTGG TTCTTGGTGC TGGCAAAACT GAAGGGTCGA TGGATGCAGC TAATCTGTTC 
AAGCCCATGT TAGCTAGAGG GCAGCTTCGA TGCATTGGTG CTACAACGCT TGAAGAATAC 
AGGAAATATG TTGAGAAAGA TGCTGCCTTT GAGAGGAGGT TCCAACAAGT CTATGTTGCG 
GAGCCAAGTG TGCCTGACAC CATTAGTATC CTTAGAGGAC TCAAGGAGAA GTATGAGGGA 
CATCATGGTG TGCGAATCCA AGACAGAGCT CTTATAAATG CTGCTCAGCT GTCTGCTCGT 
TACATAACTG GTCGGCATTT ACCGGATAAA GCAATTGATT TGGTTGATGA GGCTTGTGCG 
AATGTGAGAG TCCAGCTTGA TAGTCAACCT GAAGAGATTG ATAACCTTGA AAGGAAGAGG 
ATGCAGCTGG AAATTGAACT TCACGCCTTG GAAAGGGAGA AGGATAAAGC CAGCAAAGCT 
CGACTTATAG AGGTGCGGAA AGAGCTTGAT GACCTGAGAG ACAAGCTTCA GCCTCTCACG 
ATGAAATACA GAAAGGAGAA AGAGAGAATT GATGAGATTC GAAGGCTTAA ACAGAAAAGA 
GAAGAGCTCA TGTTTTCTTT GCAGGAGGCA GAACGAAGAT ATGACCTTGC AAGAGCTGCT 
GATCTAAGAT ATGGCGCAAT TCAAGAAGTG GAATCTGCAA TTGCCCAACT TGAAGGAACT 
TCTTCTGAAG AGAATGTGAT GCTCACAGAA AACGTTGGGC CTGAACACAT TGCTGAGGTT 
GTGAGCCGTT GGACAGGGAT TCCAGTGACG AGACTTGGCC AAAATGAGAA GGAGAGGTTG 
ATTGGTCTTG CTGATAGGTT GCATAAGCGG GTTGTGGGAC AGAATCAAGC GGTAAATGCA 
GTTTCTGAGG CAATTCTAAG GTCAAGGGCA GGACTTGGAA GGCCACAACA GCCAACTGGA 
TCATTCTTAT TCCTTGGACC AACTGGTGTT GGCAAAACTG AGCTCGCCAA GGCTCTTGCT 
GAGCAGCTGT TTGATGATGA AAACCTCTTA GTTCGGATTG ATATGTCGGA ATATATGGAA 
CAACACTCTG TCTCTCGCCT CATTGGGGCA CCACCAGGGT ATGTTGGTCA CGAGGAAGGT 
GGACAACTAA CTGAGGCTGT GAGGAGGCGA CCTTATTGTG TCATACTCTT TGATGAAGTG 
GAGAAGGCTC ATGTTGCTGT CTTCAACACT CTGCTCCAAG TTTTGGATGA TGGTCGATTG 
ACAGACGGGC AAGGCAGGAC AGTCGATTTC AGGAACTCGG TGATAATCAT GACATCAAAC 
CTTGGTGCTG AACACCTCCT TGCAGGGCTA ACTGGGAAAG TAACAATGGA AGTGGCCCGG 
GACTGTGTGA TGCGGGAGGT GAGGAAACAC TTCAGACCAG AGCTCTTGAA CAGGCTTGAC 
GAGATTGTGG TGTTCGACCC CCTTTCACAT GACCAGTTGA GGAAAGTAGC TCGGCTTCAA 
ATGAAAGACG TTGCTGTCCG GCTTGCTGAA AGAGGAGTTG CTTTGGCAGT CACTGATGCT 
GCTTTGGACT ATATCTTGGC AGAGAGTTAT GACCCGGTAA GTCTCTTCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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HSP101 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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