AT1G72700 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT1G72700
Entrez Gene ID	843602
Full Name	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
Synonyms	F28P22.11,F28P22_11
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_105929.5	NP_177414.1	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
NM_001334546.1	NP_001322800.1	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
NM_001334547.1	NP_001322799.1	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP8B4	NP_079113.2
Pan troglodytes (chimpanzee)	ATP8B4	XP_001168022.1
Arabidopsis thaliana (thale cress)	ACA.l	NP_172780.1
Arabidopsis thaliana (thale cress)	AT3G13900	NP_188006.4
Arabidopsis thaliana (thale cress)	AT1G68710	NP_177038.1
Mus musculus (house mouse)	Atp8b4	NP_001074413.2
Rattus norvegicus (Norway rat)	Atp8b4	XP_006235000.1
Danio rerio (zebrafish)	si:ch211-167m1.4	XP_687715.4
Arabidopsis thaliana (thale cress)	AT3G27870	NP_189425.2
	ATP8B4	XP_002804819.1
Bos taurus (cattle)	LOC100137953	XP_005211944.1
Arabidopsis thaliana (thale cress)	AT1G26130	NP_001185096.1
Arabidopsis thaliana (thale cress)	AT3G25610	NP_189189.1
Xenopus tropicalis (tropical clawed frog)	atp8b4	XP_002936618.2
Arabidopsis thaliana (thale cress)	AT1G17500	NP_173193.2
Canis lupus familiaris (dog)	ATP8B4	XP_005638457.1
Arabidopsis thaliana (thale cress)	AT1G54280	NP_175830.1
Arabidopsis thaliana (thale cress)	AT1G72700	NP_177414.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT1G72700 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT1G72700 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb27777	NM_001334547.1 Latest version!	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb27777	NM_001334546.1 Latest version!	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb27777	NM_105929.5 Latest version!	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT1G72700 ( NM_105929.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_177414.1 Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.

Price & Availability↑

CloneID	OAb27777
Clone ID Related Accession (Same CDS sequence)	NM_105929.5 , NM_001334546.1 , NM_001334547.1
Accession Version	NM_105929.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_105929.4.

ATGGCTCGAG GTAGAATAAG ATCGAAGCTG CGACTAAGCC TTCTTTATAC ATTTGGATGT 
CTTAGGCCAG CTACGCTTGA AGGTCAAGAT TCTCAACCAA TCCAAGGTCC GGGTTTCAGC 
CGGACTGTGT TCTGTAATCA GCCTCATATG CACAAGAAGA AACCTTTGAG ATATCGTTCT 
AATTATGTCT CTACGACGAG GTACAATTTG ATCACTTTCT TTCCTAAGTC GTTGTACGAG 
CAGTTCCACC GTGCTGCGAA TCTCTACTTC TTGGTAGCTG CGATTCTCTC GGTGTTTCCT 
CTATCACCAT TCAACAAGTG GAGCATGATT GCTCCTTTGG TCTTTGTTGT TGGGCTTAGT 
ATGTTAAAAG AGGCTCTAGA AGATTGGCGT AGGTTTATGC AAGACGTGAA GATTAATGCG 
CGGAAAACTT GTGTTCATAA AAGTGACGGT GTGTTTCGTC AGAGAAAGTG GAAGAAGGTT 
AGCGTTGGGG ATATTGTGAA AGTGGAGAAG GATGAGTTTT TCCCTGCTGA TTTGCTTCTT 
TTGTCATCGA GTTACGAGGA TGGGATTTGC TACGTTGAGA CTATGAACTT AGATGGTGAA 
ACAAACTTGA AAGTGAAAAG ATCTTTGGAA GTGTCACTGC CACTAGATGA TGATGAATCT 
TTCAAGAATT TCATGGCGAC GATAAGATGT GAAGATCCAA ACCCGAATCT CTATACCTTT 
GTTGGCAATC TTGAGTTTGA GAGGCAAACA TTTCCTCTGG ATCCAAGTCA GATTCTCTTA 
AGAGACTCAA AGCTTAGGAA TACAACCTAT GTTTATGGAG TTGTGGTATT TACTGGTTTT 
GATACCAAAG TTATGCAGAA TTCAACAAAG TCGCCTTCCA AGAGAAGCAG AATAGAAAGG 
ACAATGGACT ACATCATCTA CACTCTTCTT GTCCTACTTA TTTTAATCTC TTGCATCAGC 
TCATCAGGAT TTGCTTGGGA GACAGAGTTT CACATGCCAA AAATGTGGTA CTTACGACCT 
GGCGAGCCTA TAGACTTCAC AAATCCGATC AATCCAATCT ATGCTGGGGT TGTTCATCTG 
ATCACTGCTC TATTGCTTTA TGGATATTTG ATTCCAATCT CTCTTTATGT TTCGATCGAG 
GTTGTCAAAG TCTGGCAAGC AAGTTTCATC AATCAAGACT TACACATGTA TGATGATGAG 
AGTGGAGTCC CTGCAAATGC GCGCACATCG AATCTAAACG AAGAGCTTGG TCAGGTTCAT 
ACTATCCTCT CTGACAAAAC AGGAACTTTG ACATGTAATC AGATGGATTT CTTGAAATGC 
TCCATTGCTG GTACTTCTTA TGGTGTGCGT TCCAGCGAAG TAGAAGTTGC TGCTGCAAAG 
CAGATGGCTG TGGATCTTGA AGAGCACGGT GAGATATCGA GTACTCCTCA GTCTCAGACT 
AAAGTGTATG GTACATGGGA CAGTAGCCGT ACGCAAGAAA TCGAGGTGGA AGGGGATAAT 
AACTATAATA CTCCTAGAGC TCCTATAAAG GGATTTGGTT TTGAGGATAA CAGACTTATG 
AATGGTAATT GGTTGAGGGA ATCACAACCA AATGACATTT TGCAGTTCTT CCGCATATTA 
GCTATTTGTC ACACAGCTAT TCCCGAGCTG AACGAGGAGA CTGGCAAGTA CACTTATGAA 
GCAGAGTCAC CTGATGAGGC TTCTTTTCTT GCTGCTGCTA GAGAGTTTGG TTTTGAGTTC 
TTCAAGAGAA CCCAGTCAAG TGTTTTTATC CGCGAGAGGT TCTCTGGTTC AGGGCAAATA 
ATCGAAAGGG AGTACAAAGT TCTGAATTTG TTGGAATTTA CGAGCAAAAG AAAGCGAATG 
ACAGTAATTG TACGCGATGA GGAAGGGCAG ATTCTTCTAC TATGCAAAGG AGCTGACAGC 
ATCATCTTTG AGCGGTTGGC GAAGAATGGA AAGACATACT TGGGACCTAC CACTAGGCAT 
TTAACTGAAT ATGGAGAAGC CGGACTCCGT ACACTCGCAC TTGCTTACAG AAAGCTTGAT 
GAGGATGAAT ATGCAGCTTG GAACTCTGAG TTTCTTAAGG CCAAAACTTC AATAGGATCT 
GATAGAGATG AGCTGCTTGA GACGGGAGCA GATATGATTG AGAAAGAACT TATCCTTATA 
GGAGCTACTG CTGTGGAGGA CAAACTCCAG AAAGGGGTGC CCCAATGCAT AGATAAACTT 
GCTCAAGCTG GGCTCAAGTT ATGGGTTTTA ACTGGGGACA AAATGGAAAC TGCAATCAAC 
ATTGGATTCG CGTGTAGTTT ACTACGACAA GGCATGAGAC AGATCTGCAT AACATCAATG 
AATTCAGAAG GAGGATCCCA AGATTCAAAA AGGGTTGTGA AAGAGAACAT TTTGAATCAA 
CTCACAAAAG CTGTACAAAT GGTGAAGCTT GAGAAGGATC CGCATGCTGC ATTTGCTTTG 
ATTATTGACG GGAAAACTCT AACTTATGCC TTGGAGGATG ATATGAAGTA TCAATTCTTG 
GCTTTGGCAG TGGATTGTGC ATCAGTCATT TGCTGTCGAG TGTCTCCCAA GCAGAAGGCT 
TTGGTTGTTA GGTTGGTTAA AGAGGGAACT GGAAAAACCA CATTGGCAAT AGGTGATGGT 
GCAAATGATG TTGGGATGAT TCAAGAAGCT GACATTGGTG TAGGAATTAG TGGGGTTGAA 
GGAATGCAGG CTGTTATGGC TAGTGACTTT TCGATTGCCC AGTTCCGGTT TCTGGAACGA 
TTACTCGTCG TCCACGGACA CTGGTGCTAC AAAAGGATAG CTCAGATGAT TTGCTATTTC 
TTCTACAAAA ACATAGCATT TGGTCTCACT CTCTTCTACT TTGAGGCTTT CACCGGGTTT 
TCCGGGCAAT CAGTATACAA TGACTACTAC TTGTTGCTCT TCAATGTTGT CCTTACCTCA 
TTGCCAGTAA TCGCCCTTGG TGTCTTTGAA CAGGATGTTT CTTCCGAGAT CTGCTTACAG 
TTCCCAGCTT TATACCAACA AGGCACAAAG AACCTCTTCT TTGATTGGTC AAGAATACTT 
GGATGGATGT GCAATGGCGT CTATGCATCT CTTGTCATAT TCTTCCTCAA CATCGGAATC 
ATTTACTCTC AAGCTTTCAG AGACAATGGC CAAACAGCTG ACATGGATGC TGTTGGCACA 
ACCATGTTCA CTTGCATAAT CTGGGCAGCT AATGTTCAAA TCGCGTTAAC CATGTCCCAT 
TTCACTTGGA TCCAACACGT TTTGATATGG GGAAGCATCG GTATGTGGTA TCTCTTTGTG 
GCTATCTACA GTATGATGCC TCCGAGTTAT TCTGGAAACA TTTACAGGAT TCTTGATGAG 
ATTCTTGCTC CTGCACCTAT CTACTGGATG GCTACATTGT TGGTCACAGT GGCTGCAGTT 
CTTCCTTACG TTGCTCACAT TGCATTCCAG AGATTCTTGA ATCCATTGGA TCATCATATC 
ATCCAAGAGA TCAAATACTA CGGAAGAGAT ATTGAAGATG CGAGATTGTG GACCCGAGAG 
CGTACAAAAG CCCGAGAAAA GACAAAGATC GGATTCACAG CTAGAGTCGA TGCGAAAATC 
AGGCATCTAA GGTCGAAACT GAACAAGAAA CAGTCAAATC TCTCACATTT TTCAGCTCAA 
GACGCAATGT CACCTAGATC ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_177414.1
CDS	600..4286
Translation	MARGRIRSKLRLSLLYTFGCLRPATLEGQDSQPIQGPGFSRTVFCNQPHMHKKKPLRYRSNYVSTTRYNLITFFPKSLYEQFHRAANLYFLVAAILSVFPLSPFNKWSMIAPLVFVVGLSMLKEALEDWRRFMQDVKINARKTCVHKSDGVFRQRKWKKVSVGDIVKVEKDEFFPADLLLLSSSYEDGICYVETMNLDGETNLKVKRSLEVSLPLDDDESFKNFMATIRCEDPNPNLYTFVGNLEFERQTFPLDPSQILLRDSKLRNTTYVYGVVVFTGFDTKVMQNSTKSPSKRSRIERTMDYIIYTLLVLLILISCISSSGFAWETEFHMPKMWYLRPGEPIDFTNPINPIYAGVVHLITALLLYGYLIPISLYVSIEVVKVWQASFINQDLHMYDDESGVPANARTSNLNEELGQVHTILSDKTGTLTCNQMDFLKCSIAGTSYGVRSSEVEVAAAKQMAVDLEEHGEISSTPQSQTKVYGTWDSSRTQEIEVEGDNNYNTPRAPIKGFGFEDNRLMNGNWLRESQPNDILQFFRILAICHTAIPELNEETGKYTYEAESPDEASFLAAAREFGFEFFKRTQSSVFIRERFSGSGQIIEREYKVLNLLEFTSKRKRMTVIVRDEEGQILLLCKGADSIIFERLAKNGKTYLGPTTRHLTEYGEAGLRTLALAYRKLDEDEYAAWNSEFLKAKTSIGSDRDELLETGADMIEKELILIGATAVEDKLQKGVPQCIDKLAQAGLKLWVLTGDKMETAINIGFACSLLRQGMRQICITSMNSEGGSQDSKRVVKENILNQLTKAVQMVKLEKDPHAAFALIIDGKTLTYALEDDMKYQFLALAVDCASVICCRVSPKQKALVVRLVKEGTGKTTLAIGDGANDVGMIQEADIGVGISGVEGMQAVMASDFSIAQFRFLERLLVVHGHWCYKRIAQMICYFFYKNIAFGLTLFYFEAFTGFSGQSVYNDYYLLLFNVVLTSLPVIALGVFEQDVSSEICLQFPALYQQGTKNLFFDWSRILGWMCNGVYASLVIFFLNIGIIYSQAFRDNGQTADMDAVGTTMFTCIIWAANVQIALTMSHFTWIQHVLIWGSIGMWYLFVAIYSMMPPSYSGNIYRILDEILAPAPIYWMATLLVTVAAVLPYVAHIAFQRFLNPLDHHIIQEIKYYGRDIEDARLWTRERTKAREKTKIGFTARVDAKIRHLRSKLNKKQSNLSHFSAQDAMSPRSL

Target ORF information:

RefSeq Version	NM_105929.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_105929.5

ORF Insert Sequence:

ATGGCTCGAG GTAGAATAAG ATCGAAGCTG CGACTAAGCC TTCTTTATAC ATTTGGATGT 
CTTAGGCCAG CTACGCTTGA AGGTCAAGAT TCTCAACCAA TCCAAGGTCC GGGTTTCAGC 
CGGACTGTGT TCTGTAATCA GCCTCATATG CACAAGAAGA AACCTTTGAG ATATCGTTCT 
AATTATGTCT CTACGACGAG GTACAATTTG ATCACTTTCT TTCCTAAGTC GTTGTACGAG 
CAGTTCCACC GTGCTGCGAA TCTCTACTTC TTGGTAGCTG CGATTCTCTC GGTGTTTCCT 
CTATCACCAT TCAACAAGTG GAGCATGATT GCTCCTTTGG TCTTTGTTGT TGGGCTTAGT 
ATGTTAAAAG AGGCTCTAGA AGATTGGCGT AGGTTTATGC AAGACGTGAA GATTAATGCG 
CGGAAAACTT GTGTTCATAA AAGTGACGGT GTGTTTCGTC AGAGAAAGTG GAAGAAGGTT 
AGCGTTGGGG ATATTGTGAA AGTGGAGAAG GATGAGTTTT TCCCTGCTGA TTTGCTTCTT 
TTGTCATCGA GTTACGAGGA TGGGATTTGC TACGTTGAGA CTATGAACTT AGATGGTGAA 
ACAAACTTGA AAGTGAAAAG ATCTTTGGAA GTGTCACTGC CACTAGATGA TGATGAATCT 
TTCAAGAATT TCATGGCGAC GATAAGATGT GAAGATCCAA ACCCGAATCT CTATACCTTT 
GTTGGCAATC TTGAGTTTGA GAGGCAAACA TTTCCTCTGG ATCCAAGTCA GATTCTCTTA 
AGAGACTCAA AGCTTAGGAA TACAACCTAT GTTTATGGAG TTGTGGTATT TACTGGTTTT 
GATACCAAAG TTATGCAGAA TTCAACAAAG TCGCCTTCCA AGAGAAGCAG AATAGAAAGG 
ACAATGGACT ACATCATCTA CACTCTTCTT GTCCTACTTA TTTTAATCTC TTGCATCAGC 
TCATCAGGAT TTGCTTGGGA GACAGAGTTT CACATGCCAA AAATGTGGTA CTTACGACCT 
GGCGAGCCTA TAGACTTCAC AAATCCGATC AATCCAATCT ATGCTGGGGT TGTTCATCTG 
ATCACTGCTC TATTGCTTTA TGGATATTTG ATTCCAATCT CTCTTTATGT TTCGATCGAG 
GTTGTCAAAG TCTGGCAAGC AAGTTTCATC AATCAAGACT TACACATGTA TGATGATGAG 
AGTGGAGTCC CTGCAAATGC GCGCACATCG AATCTAAACG AAGAGCTTGG TCAGGTTCAT 
ACTATCCTCT CTGACAAAAC AGGAACTTTG ACATGTAATC AGATGGATTT CTTGAAATGC 
TCCATTGCTG GTACTTCTTA TGGTGTGCGT TCCAGCGAAG TAGAAGTTGC TGCTGCAAAG 
CAGATGGCTG TGGATCTTGA AGAGCACGGT GAGATATCGA GTACTCCTCA GTCTCAGACT 
AAAGTGTATG GTACATGGGA CAGTAGCCGT ACGCAAGAAA TCGAGGTGGA AGGGGATAAT 
AACTATAATA CTCCTAGAGC TCCTATAAAG GGATTTGGTT TTGAGGATAA CAGACTTATG 
AATGGTAATT GGTTGAGGGA ATCACAACCA AATGACATTT TGCAGTTCTT CCGCATATTA 
GCTATTTGTC ACACAGCTAT TCCCGAGCTG AACGAGGAGA CTGGCAAGTA CACTTATGAA 
GCAGAGTCAC CTGATGAGGC TTCTTTTCTT GCTGCTGCTA GAGAGTTTGG TTTTGAGTTC 
TTCAAGAGAA CCCAGTCAAG TGTTTTTATC CGCGAGAGGT TCTCTGGTTC AGGGCAAATA 
ATCGAAAGGG AGTACAAAGT TCTGAATTTG TTGGAATTTA CGAGCAAAAG AAAGCGAATG 
ACAGTAATTG TACGCGATGA GGAAGGGCAG ATTCTTCTAC TATGCAAAGG AGCTGACAGC 
ATCATCTTTG AGCGGTTGGC GAAGAATGGA AAGACATACT TGGGACCTAC CACTAGGCAT 
TTAACTGAAT ATGGAGAAGC CGGACTCCGT ACACTCGCAC TTGCTTACAG AAAGCTTGAT 
GAGGATGAAT ATGCAGCTTG GAACTCTGAG TTTCTTAAGG CCAAAACTTC AATAGGATCT 
GATAGAGATG AGCTGCTTGA GACGGGAGCA GATATGATTG AGAAAGAACT TATCCTTATA 
GGAGCTACTG CTGTGGAGGA CAAACTCCAG AAAGGGGTGC CCCAATGCAT AGATAAACTT 
GCTCAAGCTG GGCTCAAGTT ATGGGTTTTA ACTGGGGACA AAATGGAAAC TGCAATCAAC 
ATTGGATTCG CGTGTAGTTT ACTACGACAA GGCATGAGAC AGATCTGCAT AACATCAATG 
AATTCAGAAG GAGGATCCCA AGATTCAAAA AGGGTTGTGA AAGAGAACAT TTTGAATCAA 
CTCACAAAAG CTGTACAAAT GGTGAAGCTT GAGAAGGATC CGCATGCTGC ATTTGCTTTG 
ATTATTGACG GGAAAACTCT AACTTATGCC TTGGAGGATG ATATGAAGTA TCAATTCTTG 
GCTTTGGCAG TGGATTGTGC ATCAGTCATT TGCTGTCGAG TGTCTCCCAA GCAGAAGGCT 
TTGGTTGTTA GGTTGGTTAA AGAGGGAACT GGAAAAACCA CATTGGCAAT AGGTGATGGT 
GCAAATGATG TTGGGATGAT TCAAGAAGCT GACATTGGTG TAGGAATTAG TGGGGTTGAA 
GGAATGCAGG CTGTTATGGC TAGTGACTTT TCGATTGCCC AGTTCCGGTT TCTGGAACGA 
TTACTCGTCG TCCACGGACA CTGGTGCTAC AAAAGGATAG CTCAGATGAT TTGCTATTTC 
TTCTACAAAA ACATAGCATT TGGTCTCACT CTCTTCTACT TTGAGGCTTT CACCGGGTTT 
TCCGGGCAAT CAGTATACAA TGACTACTAC TTGTTGCTCT TCAATGTTGT CCTTACCTCA 
TTGCCAGTAA TCGCCCTTGG TGTCTTTGAA CAGGATGTTT CTTCCGAGAT CTGCTTACAG 
TTCCCAGCTT TATACCAACA AGGCACAAAG AACCTCTTCT TTGATTGGTC AAGAATACTT 
GGATGGATGT GCAATGGCGT CTATGCATCT CTTGTCATAT TCTTCCTCAA CATCGGAATC 
ATTTACTCTC AAGCTTTCAG AGACAATGGC CAAACAGCTG ACATGGATGC TGTTGGCACA 
ACCATGTTCA CTTGCATAAT CTGGGCAGCT AATGTTCAAA TCGCGTTAAC CATGTCCCAT 
TTCACTTGGA TCCAACACGT TTTGATATGG GGAAGCATCG GTATGTGGTA TCTCTTTGTG 
GCTATCTACA GTATGATGCC TCCGAGTTAT TCTGGAAACA TTTACAGGAT TCTTGATGAG 
ATTCTTGCTC CTGCACCTAT CTACTGGATG GCTACATTGT TGGTCACAGT GGCTGCAGTT 
CTTCCTTACG TTGCTCACAT TGCATTCCAG AGATTCTTGA ATCCATTGGA TCATCATATC 
ATCCAAGAGA TCAAATACTA CGGAAGAGAT ATTGAAGATG CGAGATTGTG GACCCGAGAG 
CGTACAAAAG CCCGAGAAAA GACAAAGATC GGATTCACAG CTAGAGTCGA TGCGAAAATC 
AGGCATCTAA GGTCGAAACT GAACAAGAAA CAGTCAAATC TCTCACATTT TTCAGCTCAA 
GACGCAATGT CACCTAGATC ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT1G72700 ( NM_001334546.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322800.1 Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.

Price & Availability↑

CloneID	OAb27777
Clone ID Related Accession (Same CDS sequence)	NM_105929.5 , NM_001334546.1 , NM_001334547.1
Accession Version	NM_001334546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCTCGAG GTAGAATAAG ATCGAAGCTG CGACTAAGCC TTCTTTATAC ATTTGGATGT 
CTTAGGCCAG CTACGCTTGA AGGTCAAGAT TCTCAACCAA TCCAAGGTCC GGGTTTCAGC 
CGGACTGTGT TCTGTAATCA GCCTCATATG CACAAGAAGA AACCTTTGAG ATATCGTTCT 
AATTATGTCT CTACGACGAG GTACAATTTG ATCACTTTCT TTCCTAAGTC GTTGTACGAG 
CAGTTCCACC GTGCTGCGAA TCTCTACTTC TTGGTAGCTG CGATTCTCTC GGTGTTTCCT 
CTATCACCAT TCAACAAGTG GAGCATGATT GCTCCTTTGG TCTTTGTTGT TGGGCTTAGT 
ATGTTAAAAG AGGCTCTAGA AGATTGGCGT AGGTTTATGC AAGACGTGAA GATTAATGCG 
CGGAAAACTT GTGTTCATAA AAGTGACGGT GTGTTTCGTC AGAGAAAGTG GAAGAAGGTT 
AGCGTTGGGG ATATTGTGAA AGTGGAGAAG GATGAGTTTT TCCCTGCTGA TTTGCTTCTT 
TTGTCATCGA GTTACGAGGA TGGGATTTGC TACGTTGAGA CTATGAACTT AGATGGTGAA 
ACAAACTTGA AAGTGAAAAG ATCTTTGGAA GTGTCACTGC CACTAGATGA TGATGAATCT 
TTCAAGAATT TCATGGCGAC GATAAGATGT GAAGATCCAA ACCCGAATCT CTATACCTTT 
GTTGGCAATC TTGAGTTTGA GAGGCAAACA TTTCCTCTGG ATCCAAGTCA GATTCTCTTA 
AGAGACTCAA AGCTTAGGAA TACAACCTAT GTTTATGGAG TTGTGGTATT TACTGGTTTT 
GATACCAAAG TTATGCAGAA TTCAACAAAG TCGCCTTCCA AGAGAAGCAG AATAGAAAGG 
ACAATGGACT ACATCATCTA CACTCTTCTT GTCCTACTTA TTTTAATCTC TTGCATCAGC 
TCATCAGGAT TTGCTTGGGA GACAGAGTTT CACATGCCAA AAATGTGGTA CTTACGACCT 
GGCGAGCCTA TAGACTTCAC AAATCCGATC AATCCAATCT ATGCTGGGGT TGTTCATCTG 
ATCACTGCTC TATTGCTTTA TGGATATTTG ATTCCAATCT CTCTTTATGT TTCGATCGAG 
GTTGTCAAAG TCTGGCAAGC AAGTTTCATC AATCAAGACT TACACATGTA TGATGATGAG 
AGTGGAGTCC CTGCAAATGC GCGCACATCG AATCTAAACG AAGAGCTTGG TCAGGTTCAT 
ACTATCCTCT CTGACAAAAC AGGAACTTTG ACATGTAATC AGATGGATTT CTTGAAATGC 
TCCATTGCTG GTACTTCTTA TGGTGTGCGT TCCAGCGAAG TAGAAGTTGC TGCTGCAAAG 
CAGATGGCTG TGGATCTTGA AGAGCACGGT GAGATATCGA GTACTCCTCA GTCTCAGACT 
AAAGTGTATG GTACATGGGA CAGTAGCCGT ACGCAAGAAA TCGAGGTGGA AGGGGATAAT 
AACTATAATA CTCCTAGAGC TCCTATAAAG GGATTTGGTT TTGAGGATAA CAGACTTATG 
AATGGTAATT GGTTGAGGGA ATCACAACCA AATGACATTT TGCAGTTCTT CCGCATATTA 
GCTATTTGTC ACACAGCTAT TCCCGAGCTG AACGAGGAGA CTGGCAAGTA CACTTATGAA 
GCAGAGTCAC CTGATGAGGC TTCTTTTCTT GCTGCTGCTA GAGAGTTTGG TTTTGAGTTC 
TTCAAGAGAA CCCAGTCAAG TGTTTTTATC CGCGAGAGGT TCTCTGGTTC AGGGCAAATA 
ATCGAAAGGG AGTACAAAGT TCTGAATTTG TTGGAATTTA CGAGCAAAAG AAAGCGAATG 
ACAGTAATTG TACGCGATGA GGAAGGGCAG ATTCTTCTAC TATGCAAAGG AGCTGACAGC 
ATCATCTTTG AGCGGTTGGC GAAGAATGGA AAGACATACT TGGGACCTAC CACTAGGCAT 
TTAACTGAAT ATGGAGAAGC CGGACTCCGT ACACTCGCAC TTGCTTACAG AAAGCTTGAT 
GAGGATGAAT ATGCAGCTTG GAACTCTGAG TTTCTTAAGG CCAAAACTTC AATAGGATCT 
GATAGAGATG AGCTGCTTGA GACGGGAGCA GATATGATTG AGAAAGAACT TATCCTTATA 
GGAGCTACTG CTGTGGAGGA CAAACTCCAG AAAGGGGTGC CCCAATGCAT AGATAAACTT 
GCTCAAGCTG GGCTCAAGTT ATGGGTTTTA ACTGGGGACA AAATGGAAAC TGCAATCAAC 
ATTGGATTCG CGTGTAGTTT ACTACGACAA GGCATGAGAC AGATCTGCAT AACATCAATG 
AATTCAGAAG GAGGATCCCA AGATTCAAAA AGGGTTGTGA AAGAGAACAT TTTGAATCAA 
CTCACAAAAG CTGTACAAAT GGTGAAGCTT GAGAAGGATC CGCATGCTGC ATTTGCTTTG 
ATTATTGACG GGAAAACTCT AACTTATGCC TTGGAGGATG ATATGAAGTA TCAATTCTTG 
GCTTTGGCAG TGGATTGTGC ATCAGTCATT TGCTGTCGAG TGTCTCCCAA GCAGAAGGCT 
TTGGTTGTTA GGTTGGTTAA AGAGGGAACT GGAAAAACCA CATTGGCAAT AGGTGATGGT 
GCAAATGATG TTGGGATGAT TCAAGAAGCT GACATTGGTG TAGGAATTAG TGGGGTTGAA 
GGAATGCAGG CTGTTATGGC TAGTGACTTT TCGATTGCCC AGTTCCGGTT TCTGGAACGA 
TTACTCGTCG TCCACGGACA CTGGTGCTAC AAAAGGATAG CTCAGATGAT TTGCTATTTC 
TTCTACAAAA ACATAGCATT TGGTCTCACT CTCTTCTACT TTGAGGCTTT CACCGGGTTT 
TCCGGGCAAT CAGTATACAA TGACTACTAC TTGTTGCTCT TCAATGTTGT CCTTACCTCA 
TTGCCAGTAA TCGCCCTTGG TGTCTTTGAA CAGGATGTTT CTTCCGAGAT CTGCTTACAG 
TTCCCAGCTT TATACCAACA AGGCACAAAG AACCTCTTCT TTGATTGGTC AAGAATACTT 
GGATGGATGT GCAATGGCGT CTATGCATCT CTTGTCATAT TCTTCCTCAA CATCGGAATC 
ATTTACTCTC AAGCTTTCAG AGACAATGGC CAAACAGCTG ACATGGATGC TGTTGGCACA 
ACCATGTTCA CTTGCATAAT CTGGGCAGCT AATGTTCAAA TCGCGTTAAC CATGTCCCAT 
TTCACTTGGA TCCAACACGT TTTGATATGG GGAAGCATCG GTATGTGGTA TCTCTTTGTG 
GCTATCTACA GTATGATGCC TCCGAGTTAT TCTGGAAACA TTTACAGGAT TCTTGATGAG 
ATTCTTGCTC CTGCACCTAT CTACTGGATG GCTACATTGT TGGTCACAGT GGCTGCAGTT 
CTTCCTTACG TTGCTCACAT TGCATTCCAG AGATTCTTGA ATCCATTGGA TCATCATATC 
ATCCAAGAGA TCAAATACTA CGGAAGAGAT ATTGAAGATG CGAGATTGTG GACCCGAGAG 
CGTACAAAAG CCCGAGAAAA GACAAAGATC GGATTCACAG CTAGAGTCGA TGCGAAAATC 
AGGCATCTAA GGTCGAAACT GAACAAGAAA CAGTCAAATC TCTCACATTT TTCAGCTCAA 
GACGCAATGT CACCTAGATC ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322800.1
CDS	851..4537
Translation	MARGRIRSKLRLSLLYTFGCLRPATLEGQDSQPIQGPGFSRTVFCNQPHMHKKKPLRYRSNYVSTTRYNLITFFPKSLYEQFHRAANLYFLVAAILSVFPLSPFNKWSMIAPLVFVVGLSMLKEALEDWRRFMQDVKINARKTCVHKSDGVFRQRKWKKVSVGDIVKVEKDEFFPADLLLLSSSYEDGICYVETMNLDGETNLKVKRSLEVSLPLDDDESFKNFMATIRCEDPNPNLYTFVGNLEFERQTFPLDPSQILLRDSKLRNTTYVYGVVVFTGFDTKVMQNSTKSPSKRSRIERTMDYIIYTLLVLLILISCISSSGFAWETEFHMPKMWYLRPGEPIDFTNPINPIYAGVVHLITALLLYGYLIPISLYVSIEVVKVWQASFINQDLHMYDDESGVPANARTSNLNEELGQVHTILSDKTGTLTCNQMDFLKCSIAGTSYGVRSSEVEVAAAKQMAVDLEEHGEISSTPQSQTKVYGTWDSSRTQEIEVEGDNNYNTPRAPIKGFGFEDNRLMNGNWLRESQPNDILQFFRILAICHTAIPELNEETGKYTYEAESPDEASFLAAAREFGFEFFKRTQSSVFIRERFSGSGQIIEREYKVLNLLEFTSKRKRMTVIVRDEEGQILLLCKGADSIIFERLAKNGKTYLGPTTRHLTEYGEAGLRTLALAYRKLDEDEYAAWNSEFLKAKTSIGSDRDELLETGADMIEKELILIGATAVEDKLQKGVPQCIDKLAQAGLKLWVLTGDKMETAINIGFACSLLRQGMRQICITSMNSEGGSQDSKRVVKENILNQLTKAVQMVKLEKDPHAAFALIIDGKTLTYALEDDMKYQFLALAVDCASVICCRVSPKQKALVVRLVKEGTGKTTLAIGDGANDVGMIQEADIGVGISGVEGMQAVMASDFSIAQFRFLERLLVVHGHWCYKRIAQMICYFFYKNIAFGLTLFYFEAFTGFSGQSVYNDYYLLLFNVVLTSLPVIALGVFEQDVSSEICLQFPALYQQGTKNLFFDWSRILGWMCNGVYASLVIFFLNIGIIYSQAFRDNGQTADMDAVGTTMFTCIIWAANVQIALTMSHFTWIQHVLIWGSIGMWYLFVAIYSMMPPSYSGNIYRILDEILAPAPIYWMATLLVTVAAVLPYVAHIAFQRFLNPLDHHIIQEIKYYGRDIEDARLWTRERTKAREKTKIGFTARVDAKIRHLRSKLNKKQSNLSHFSAQDAMSPRSL

Target ORF information:

RefSeq Version	NM_001334546.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001334546.1

ORF Insert Sequence:

ATGGCTCGAG GTAGAATAAG ATCGAAGCTG CGACTAAGCC TTCTTTATAC ATTTGGATGT 
CTTAGGCCAG CTACGCTTGA AGGTCAAGAT TCTCAACCAA TCCAAGGTCC GGGTTTCAGC 
CGGACTGTGT TCTGTAATCA GCCTCATATG CACAAGAAGA AACCTTTGAG ATATCGTTCT 
AATTATGTCT CTACGACGAG GTACAATTTG ATCACTTTCT TTCCTAAGTC GTTGTACGAG 
CAGTTCCACC GTGCTGCGAA TCTCTACTTC TTGGTAGCTG CGATTCTCTC GGTGTTTCCT 
CTATCACCAT TCAACAAGTG GAGCATGATT GCTCCTTTGG TCTTTGTTGT TGGGCTTAGT 
ATGTTAAAAG AGGCTCTAGA AGATTGGCGT AGGTTTATGC AAGACGTGAA GATTAATGCG 
CGGAAAACTT GTGTTCATAA AAGTGACGGT GTGTTTCGTC AGAGAAAGTG GAAGAAGGTT 
AGCGTTGGGG ATATTGTGAA AGTGGAGAAG GATGAGTTTT TCCCTGCTGA TTTGCTTCTT 
TTGTCATCGA GTTACGAGGA TGGGATTTGC TACGTTGAGA CTATGAACTT AGATGGTGAA 
ACAAACTTGA AAGTGAAAAG ATCTTTGGAA GTGTCACTGC CACTAGATGA TGATGAATCT 
TTCAAGAATT TCATGGCGAC GATAAGATGT GAAGATCCAA ACCCGAATCT CTATACCTTT 
GTTGGCAATC TTGAGTTTGA GAGGCAAACA TTTCCTCTGG ATCCAAGTCA GATTCTCTTA 
AGAGACTCAA AGCTTAGGAA TACAACCTAT GTTTATGGAG TTGTGGTATT TACTGGTTTT 
GATACCAAAG TTATGCAGAA TTCAACAAAG TCGCCTTCCA AGAGAAGCAG AATAGAAAGG 
ACAATGGACT ACATCATCTA CACTCTTCTT GTCCTACTTA TTTTAATCTC TTGCATCAGC 
TCATCAGGAT TTGCTTGGGA GACAGAGTTT CACATGCCAA AAATGTGGTA CTTACGACCT 
GGCGAGCCTA TAGACTTCAC AAATCCGATC AATCCAATCT ATGCTGGGGT TGTTCATCTG 
ATCACTGCTC TATTGCTTTA TGGATATTTG ATTCCAATCT CTCTTTATGT TTCGATCGAG 
GTTGTCAAAG TCTGGCAAGC AAGTTTCATC AATCAAGACT TACACATGTA TGATGATGAG 
AGTGGAGTCC CTGCAAATGC GCGCACATCG AATCTAAACG AAGAGCTTGG TCAGGTTCAT 
ACTATCCTCT CTGACAAAAC AGGAACTTTG ACATGTAATC AGATGGATTT CTTGAAATGC 
TCCATTGCTG GTACTTCTTA TGGTGTGCGT TCCAGCGAAG TAGAAGTTGC TGCTGCAAAG 
CAGATGGCTG TGGATCTTGA AGAGCACGGT GAGATATCGA GTACTCCTCA GTCTCAGACT 
AAAGTGTATG GTACATGGGA CAGTAGCCGT ACGCAAGAAA TCGAGGTGGA AGGGGATAAT 
AACTATAATA CTCCTAGAGC TCCTATAAAG GGATTTGGTT TTGAGGATAA CAGACTTATG 
AATGGTAATT GGTTGAGGGA ATCACAACCA AATGACATTT TGCAGTTCTT CCGCATATTA 
GCTATTTGTC ACACAGCTAT TCCCGAGCTG AACGAGGAGA CTGGCAAGTA CACTTATGAA 
GCAGAGTCAC CTGATGAGGC TTCTTTTCTT GCTGCTGCTA GAGAGTTTGG TTTTGAGTTC 
TTCAAGAGAA CCCAGTCAAG TGTTTTTATC CGCGAGAGGT TCTCTGGTTC AGGGCAAATA 
ATCGAAAGGG AGTACAAAGT TCTGAATTTG TTGGAATTTA CGAGCAAAAG AAAGCGAATG 
ACAGTAATTG TACGCGATGA GGAAGGGCAG ATTCTTCTAC TATGCAAAGG AGCTGACAGC 
ATCATCTTTG AGCGGTTGGC GAAGAATGGA AAGACATACT TGGGACCTAC CACTAGGCAT 
TTAACTGAAT ATGGAGAAGC CGGACTCCGT ACACTCGCAC TTGCTTACAG AAAGCTTGAT 
GAGGATGAAT ATGCAGCTTG GAACTCTGAG TTTCTTAAGG CCAAAACTTC AATAGGATCT 
GATAGAGATG AGCTGCTTGA GACGGGAGCA GATATGATTG AGAAAGAACT TATCCTTATA 
GGAGCTACTG CTGTGGAGGA CAAACTCCAG AAAGGGGTGC CCCAATGCAT AGATAAACTT 
GCTCAAGCTG GGCTCAAGTT ATGGGTTTTA ACTGGGGACA AAATGGAAAC TGCAATCAAC 
ATTGGATTCG CGTGTAGTTT ACTACGACAA GGCATGAGAC AGATCTGCAT AACATCAATG 
AATTCAGAAG GAGGATCCCA AGATTCAAAA AGGGTTGTGA AAGAGAACAT TTTGAATCAA 
CTCACAAAAG CTGTACAAAT GGTGAAGCTT GAGAAGGATC CGCATGCTGC ATTTGCTTTG 
ATTATTGACG GGAAAACTCT AACTTATGCC TTGGAGGATG ATATGAAGTA TCAATTCTTG 
GCTTTGGCAG TGGATTGTGC ATCAGTCATT TGCTGTCGAG TGTCTCCCAA GCAGAAGGCT 
TTGGTTGTTA GGTTGGTTAA AGAGGGAACT GGAAAAACCA CATTGGCAAT AGGTGATGGT 
GCAAATGATG TTGGGATGAT TCAAGAAGCT GACATTGGTG TAGGAATTAG TGGGGTTGAA 
GGAATGCAGG CTGTTATGGC TAGTGACTTT TCGATTGCCC AGTTCCGGTT TCTGGAACGA 
TTACTCGTCG TCCACGGACA CTGGTGCTAC AAAAGGATAG CTCAGATGAT TTGCTATTTC 
TTCTACAAAA ACATAGCATT TGGTCTCACT CTCTTCTACT TTGAGGCTTT CACCGGGTTT 
TCCGGGCAAT CAGTATACAA TGACTACTAC TTGTTGCTCT TCAATGTTGT CCTTACCTCA 
TTGCCAGTAA TCGCCCTTGG TGTCTTTGAA CAGGATGTTT CTTCCGAGAT CTGCTTACAG 
TTCCCAGCTT TATACCAACA AGGCACAAAG AACCTCTTCT TTGATTGGTC AAGAATACTT 
GGATGGATGT GCAATGGCGT CTATGCATCT CTTGTCATAT TCTTCCTCAA CATCGGAATC 
ATTTACTCTC AAGCTTTCAG AGACAATGGC CAAACAGCTG ACATGGATGC TGTTGGCACA 
ACCATGTTCA CTTGCATAAT CTGGGCAGCT AATGTTCAAA TCGCGTTAAC CATGTCCCAT 
TTCACTTGGA TCCAACACGT TTTGATATGG GGAAGCATCG GTATGTGGTA TCTCTTTGTG 
GCTATCTACA GTATGATGCC TCCGAGTTAT TCTGGAAACA TTTACAGGAT TCTTGATGAG 
ATTCTTGCTC CTGCACCTAT CTACTGGATG GCTACATTGT TGGTCACAGT GGCTGCAGTT 
CTTCCTTACG TTGCTCACAT TGCATTCCAG AGATTCTTGA ATCCATTGGA TCATCATATC 
ATCCAAGAGA TCAAATACTA CGGAAGAGAT ATTGAAGATG CGAGATTGTG GACCCGAGAG 
CGTACAAAAG CCCGAGAAAA GACAAAGATC GGATTCACAG CTAGAGTCGA TGCGAAAATC 
AGGCATCTAA GGTCGAAACT GAACAAGAAA CAGTCAAATC TCTCACATTT TTCAGCTCAA 
GACGCAATGT CACCTAGATC ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT1G72700 ( NM_001334547.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322799.1 Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.

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CloneID	OAb27777
Clone ID Related Accession (Same CDS sequence)	NM_105929.5 , NM_001334546.1 , NM_001334547.1
Accession Version	NM_001334547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCTCGAG GTAGAATAAG ATCGAAGCTG CGACTAAGCC TTCTTTATAC ATTTGGATGT 
CTTAGGCCAG CTACGCTTGA AGGTCAAGAT TCTCAACCAA TCCAAGGTCC GGGTTTCAGC 
CGGACTGTGT TCTGTAATCA GCCTCATATG CACAAGAAGA AACCTTTGAG ATATCGTTCT 
AATTATGTCT CTACGACGAG GTACAATTTG ATCACTTTCT TTCCTAAGTC GTTGTACGAG 
CAGTTCCACC GTGCTGCGAA TCTCTACTTC TTGGTAGCTG CGATTCTCTC GGTGTTTCCT 
CTATCACCAT TCAACAAGTG GAGCATGATT GCTCCTTTGG TCTTTGTTGT TGGGCTTAGT 
ATGTTAAAAG AGGCTCTAGA AGATTGGCGT AGGTTTATGC AAGACGTGAA GATTAATGCG 
CGGAAAACTT GTGTTCATAA AAGTGACGGT GTGTTTCGTC AGAGAAAGTG GAAGAAGGTT 
AGCGTTGGGG ATATTGTGAA AGTGGAGAAG GATGAGTTTT TCCCTGCTGA TTTGCTTCTT 
TTGTCATCGA GTTACGAGGA TGGGATTTGC TACGTTGAGA CTATGAACTT AGATGGTGAA 
ACAAACTTGA AAGTGAAAAG ATCTTTGGAA GTGTCACTGC CACTAGATGA TGATGAATCT 
TTCAAGAATT TCATGGCGAC GATAAGATGT GAAGATCCAA ACCCGAATCT CTATACCTTT 
GTTGGCAATC TTGAGTTTGA GAGGCAAACA TTTCCTCTGG ATCCAAGTCA GATTCTCTTA 
AGAGACTCAA AGCTTAGGAA TACAACCTAT GTTTATGGAG TTGTGGTATT TACTGGTTTT 
GATACCAAAG TTATGCAGAA TTCAACAAAG TCGCCTTCCA AGAGAAGCAG AATAGAAAGG 
ACAATGGACT ACATCATCTA CACTCTTCTT GTCCTACTTA TTTTAATCTC TTGCATCAGC 
TCATCAGGAT TTGCTTGGGA GACAGAGTTT CACATGCCAA AAATGTGGTA CTTACGACCT 
GGCGAGCCTA TAGACTTCAC AAATCCGATC AATCCAATCT ATGCTGGGGT TGTTCATCTG 
ATCACTGCTC TATTGCTTTA TGGATATTTG ATTCCAATCT CTCTTTATGT TTCGATCGAG 
GTTGTCAAAG TCTGGCAAGC AAGTTTCATC AATCAAGACT TACACATGTA TGATGATGAG 
AGTGGAGTCC CTGCAAATGC GCGCACATCG AATCTAAACG AAGAGCTTGG TCAGGTTCAT 
ACTATCCTCT CTGACAAAAC AGGAACTTTG ACATGTAATC AGATGGATTT CTTGAAATGC 
TCCATTGCTG GTACTTCTTA TGGTGTGCGT TCCAGCGAAG TAGAAGTTGC TGCTGCAAAG 
CAGATGGCTG TGGATCTTGA AGAGCACGGT GAGATATCGA GTACTCCTCA GTCTCAGACT 
AAAGTGTATG GTACATGGGA CAGTAGCCGT ACGCAAGAAA TCGAGGTGGA AGGGGATAAT 
AACTATAATA CTCCTAGAGC TCCTATAAAG GGATTTGGTT TTGAGGATAA CAGACTTATG 
AATGGTAATT GGTTGAGGGA ATCACAACCA AATGACATTT TGCAGTTCTT CCGCATATTA 
GCTATTTGTC ACACAGCTAT TCCCGAGCTG AACGAGGAGA CTGGCAAGTA CACTTATGAA 
GCAGAGTCAC CTGATGAGGC TTCTTTTCTT GCTGCTGCTA GAGAGTTTGG TTTTGAGTTC 
TTCAAGAGAA CCCAGTCAAG TGTTTTTATC CGCGAGAGGT TCTCTGGTTC AGGGCAAATA 
ATCGAAAGGG AGTACAAAGT TCTGAATTTG TTGGAATTTA CGAGCAAAAG AAAGCGAATG 
ACAGTAATTG TACGCGATGA GGAAGGGCAG ATTCTTCTAC TATGCAAAGG AGCTGACAGC 
ATCATCTTTG AGCGGTTGGC GAAGAATGGA AAGACATACT TGGGACCTAC CACTAGGCAT 
TTAACTGAAT ATGGAGAAGC CGGACTCCGT ACACTCGCAC TTGCTTACAG AAAGCTTGAT 
GAGGATGAAT ATGCAGCTTG GAACTCTGAG TTTCTTAAGG CCAAAACTTC AATAGGATCT 
GATAGAGATG AGCTGCTTGA GACGGGAGCA GATATGATTG AGAAAGAACT TATCCTTATA 
GGAGCTACTG CTGTGGAGGA CAAACTCCAG AAAGGGGTGC CCCAATGCAT AGATAAACTT 
GCTCAAGCTG GGCTCAAGTT ATGGGTTTTA ACTGGGGACA AAATGGAAAC TGCAATCAAC 
ATTGGATTCG CGTGTAGTTT ACTACGACAA GGCATGAGAC AGATCTGCAT AACATCAATG 
AATTCAGAAG GAGGATCCCA AGATTCAAAA AGGGTTGTGA AAGAGAACAT TTTGAATCAA 
CTCACAAAAG CTGTACAAAT GGTGAAGCTT GAGAAGGATC CGCATGCTGC ATTTGCTTTG 
ATTATTGACG GGAAAACTCT AACTTATGCC TTGGAGGATG ATATGAAGTA TCAATTCTTG 
GCTTTGGCAG TGGATTGTGC ATCAGTCATT TGCTGTCGAG TGTCTCCCAA GCAGAAGGCT 
TTGGTTGTTA GGTTGGTTAA AGAGGGAACT GGAAAAACCA CATTGGCAAT AGGTGATGGT 
GCAAATGATG TTGGGATGAT TCAAGAAGCT GACATTGGTG TAGGAATTAG TGGGGTTGAA 
GGAATGCAGG CTGTTATGGC TAGTGACTTT TCGATTGCCC AGTTCCGGTT TCTGGAACGA 
TTACTCGTCG TCCACGGACA CTGGTGCTAC AAAAGGATAG CTCAGATGAT TTGCTATTTC 
TTCTACAAAA ACATAGCATT TGGTCTCACT CTCTTCTACT TTGAGGCTTT CACCGGGTTT 
TCCGGGCAAT CAGTATACAA TGACTACTAC TTGTTGCTCT TCAATGTTGT CCTTACCTCA 
TTGCCAGTAA TCGCCCTTGG TGTCTTTGAA CAGGATGTTT CTTCCGAGAT CTGCTTACAG 
TTCCCAGCTT TATACCAACA AGGCACAAAG AACCTCTTCT TTGATTGGTC AAGAATACTT 
GGATGGATGT GCAATGGCGT CTATGCATCT CTTGTCATAT TCTTCCTCAA CATCGGAATC 
ATTTACTCTC AAGCTTTCAG AGACAATGGC CAAACAGCTG ACATGGATGC TGTTGGCACA 
ACCATGTTCA CTTGCATAAT CTGGGCAGCT AATGTTCAAA TCGCGTTAAC CATGTCCCAT 
TTCACTTGGA TCCAACACGT TTTGATATGG GGAAGCATCG GTATGTGGTA TCTCTTTGTG 
GCTATCTACA GTATGATGCC TCCGAGTTAT TCTGGAAACA TTTACAGGAT TCTTGATGAG 
ATTCTTGCTC CTGCACCTAT CTACTGGATG GCTACATTGT TGGTCACAGT GGCTGCAGTT 
CTTCCTTACG TTGCTCACAT TGCATTCCAG AGATTCTTGA ATCCATTGGA TCATCATATC 
ATCCAAGAGA TCAAATACTA CGGAAGAGAT ATTGAAGATG CGAGATTGTG GACCCGAGAG 
CGTACAAAAG CCCGAGAAAA GACAAAGATC GGATTCACAG CTAGAGTCGA TGCGAAAATC 
AGGCATCTAA GGTCGAAACT GAACAAGAAA CAGTCAAATC TCTCACATTT TTCAGCTCAA 
GACGCAATGT CACCTAGATC ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322799.1
CDS	461..4147
Translation	MARGRIRSKLRLSLLYTFGCLRPATLEGQDSQPIQGPGFSRTVFCNQPHMHKKKPLRYRSNYVSTTRYNLITFFPKSLYEQFHRAANLYFLVAAILSVFPLSPFNKWSMIAPLVFVVGLSMLKEALEDWRRFMQDVKINARKTCVHKSDGVFRQRKWKKVSVGDIVKVEKDEFFPADLLLLSSSYEDGICYVETMNLDGETNLKVKRSLEVSLPLDDDESFKNFMATIRCEDPNPNLYTFVGNLEFERQTFPLDPSQILLRDSKLRNTTYVYGVVVFTGFDTKVMQNSTKSPSKRSRIERTMDYIIYTLLVLLILISCISSSGFAWETEFHMPKMWYLRPGEPIDFTNPINPIYAGVVHLITALLLYGYLIPISLYVSIEVVKVWQASFINQDLHMYDDESGVPANARTSNLNEELGQVHTILSDKTGTLTCNQMDFLKCSIAGTSYGVRSSEVEVAAAKQMAVDLEEHGEISSTPQSQTKVYGTWDSSRTQEIEVEGDNNYNTPRAPIKGFGFEDNRLMNGNWLRESQPNDILQFFRILAICHTAIPELNEETGKYTYEAESPDEASFLAAAREFGFEFFKRTQSSVFIRERFSGSGQIIEREYKVLNLLEFTSKRKRMTVIVRDEEGQILLLCKGADSIIFERLAKNGKTYLGPTTRHLTEYGEAGLRTLALAYRKLDEDEYAAWNSEFLKAKTSIGSDRDELLETGADMIEKELILIGATAVEDKLQKGVPQCIDKLAQAGLKLWVLTGDKMETAINIGFACSLLRQGMRQICITSMNSEGGSQDSKRVVKENILNQLTKAVQMVKLEKDPHAAFALIIDGKTLTYALEDDMKYQFLALAVDCASVICCRVSPKQKALVVRLVKEGTGKTTLAIGDGANDVGMIQEADIGVGISGVEGMQAVMASDFSIAQFRFLERLLVVHGHWCYKRIAQMICYFFYKNIAFGLTLFYFEAFTGFSGQSVYNDYYLLLFNVVLTSLPVIALGVFEQDVSSEICLQFPALYQQGTKNLFFDWSRILGWMCNGVYASLVIFFLNIGIIYSQAFRDNGQTADMDAVGTTMFTCIIWAANVQIALTMSHFTWIQHVLIWGSIGMWYLFVAIYSMMPPSYSGNIYRILDEILAPAPIYWMATLLVTVAAVLPYVAHIAFQRFLNPLDHHIIQEIKYYGRDIEDARLWTRERTKAREKTKIGFTARVDAKIRHLRSKLNKKQSNLSHFSAQDAMSPRSL

Target ORF information:

RefSeq Version	NM_001334547.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G72700), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001334547.1

ORF Insert Sequence:

ATGGCTCGAG GTAGAATAAG ATCGAAGCTG CGACTAAGCC TTCTTTATAC ATTTGGATGT 
CTTAGGCCAG CTACGCTTGA AGGTCAAGAT TCTCAACCAA TCCAAGGTCC GGGTTTCAGC 
CGGACTGTGT TCTGTAATCA GCCTCATATG CACAAGAAGA AACCTTTGAG ATATCGTTCT 
AATTATGTCT CTACGACGAG GTACAATTTG ATCACTTTCT TTCCTAAGTC GTTGTACGAG 
CAGTTCCACC GTGCTGCGAA TCTCTACTTC TTGGTAGCTG CGATTCTCTC GGTGTTTCCT 
CTATCACCAT TCAACAAGTG GAGCATGATT GCTCCTTTGG TCTTTGTTGT TGGGCTTAGT 
ATGTTAAAAG AGGCTCTAGA AGATTGGCGT AGGTTTATGC AAGACGTGAA GATTAATGCG 
CGGAAAACTT GTGTTCATAA AAGTGACGGT GTGTTTCGTC AGAGAAAGTG GAAGAAGGTT 
AGCGTTGGGG ATATTGTGAA AGTGGAGAAG GATGAGTTTT TCCCTGCTGA TTTGCTTCTT 
TTGTCATCGA GTTACGAGGA TGGGATTTGC TACGTTGAGA CTATGAACTT AGATGGTGAA 
ACAAACTTGA AAGTGAAAAG ATCTTTGGAA GTGTCACTGC CACTAGATGA TGATGAATCT 
TTCAAGAATT TCATGGCGAC GATAAGATGT GAAGATCCAA ACCCGAATCT CTATACCTTT 
GTTGGCAATC TTGAGTTTGA GAGGCAAACA TTTCCTCTGG ATCCAAGTCA GATTCTCTTA 
AGAGACTCAA AGCTTAGGAA TACAACCTAT GTTTATGGAG TTGTGGTATT TACTGGTTTT 
GATACCAAAG TTATGCAGAA TTCAACAAAG TCGCCTTCCA AGAGAAGCAG AATAGAAAGG 
ACAATGGACT ACATCATCTA CACTCTTCTT GTCCTACTTA TTTTAATCTC TTGCATCAGC 
TCATCAGGAT TTGCTTGGGA GACAGAGTTT CACATGCCAA AAATGTGGTA CTTACGACCT 
GGCGAGCCTA TAGACTTCAC AAATCCGATC AATCCAATCT ATGCTGGGGT TGTTCATCTG 
ATCACTGCTC TATTGCTTTA TGGATATTTG ATTCCAATCT CTCTTTATGT TTCGATCGAG 
GTTGTCAAAG TCTGGCAAGC AAGTTTCATC AATCAAGACT TACACATGTA TGATGATGAG 
AGTGGAGTCC CTGCAAATGC GCGCACATCG AATCTAAACG AAGAGCTTGG TCAGGTTCAT 
ACTATCCTCT CTGACAAAAC AGGAACTTTG ACATGTAATC AGATGGATTT CTTGAAATGC 
TCCATTGCTG GTACTTCTTA TGGTGTGCGT TCCAGCGAAG TAGAAGTTGC TGCTGCAAAG 
CAGATGGCTG TGGATCTTGA AGAGCACGGT GAGATATCGA GTACTCCTCA GTCTCAGACT 
AAAGTGTATG GTACATGGGA CAGTAGCCGT ACGCAAGAAA TCGAGGTGGA AGGGGATAAT 
AACTATAATA CTCCTAGAGC TCCTATAAAG GGATTTGGTT TTGAGGATAA CAGACTTATG 
AATGGTAATT GGTTGAGGGA ATCACAACCA AATGACATTT TGCAGTTCTT CCGCATATTA 
GCTATTTGTC ACACAGCTAT TCCCGAGCTG AACGAGGAGA CTGGCAAGTA CACTTATGAA 
GCAGAGTCAC CTGATGAGGC TTCTTTTCTT GCTGCTGCTA GAGAGTTTGG TTTTGAGTTC 
TTCAAGAGAA CCCAGTCAAG TGTTTTTATC CGCGAGAGGT TCTCTGGTTC AGGGCAAATA 
ATCGAAAGGG AGTACAAAGT TCTGAATTTG TTGGAATTTA CGAGCAAAAG AAAGCGAATG 
ACAGTAATTG TACGCGATGA GGAAGGGCAG ATTCTTCTAC TATGCAAAGG AGCTGACAGC 
ATCATCTTTG AGCGGTTGGC GAAGAATGGA AAGACATACT TGGGACCTAC CACTAGGCAT 
TTAACTGAAT ATGGAGAAGC CGGACTCCGT ACACTCGCAC TTGCTTACAG AAAGCTTGAT 
GAGGATGAAT ATGCAGCTTG GAACTCTGAG TTTCTTAAGG CCAAAACTTC AATAGGATCT 
GATAGAGATG AGCTGCTTGA GACGGGAGCA GATATGATTG AGAAAGAACT TATCCTTATA 
GGAGCTACTG CTGTGGAGGA CAAACTCCAG AAAGGGGTGC CCCAATGCAT AGATAAACTT 
GCTCAAGCTG GGCTCAAGTT ATGGGTTTTA ACTGGGGACA AAATGGAAAC TGCAATCAAC 
ATTGGATTCG CGTGTAGTTT ACTACGACAA GGCATGAGAC AGATCTGCAT AACATCAATG 
AATTCAGAAG GAGGATCCCA AGATTCAAAA AGGGTTGTGA AAGAGAACAT TTTGAATCAA 
CTCACAAAAG CTGTACAAAT GGTGAAGCTT GAGAAGGATC CGCATGCTGC ATTTGCTTTG 
ATTATTGACG GGAAAACTCT AACTTATGCC TTGGAGGATG ATATGAAGTA TCAATTCTTG 
GCTTTGGCAG TGGATTGTGC ATCAGTCATT TGCTGTCGAG TGTCTCCCAA GCAGAAGGCT 
TTGGTTGTTA GGTTGGTTAA AGAGGGAACT GGAAAAACCA CATTGGCAAT AGGTGATGGT 
GCAAATGATG TTGGGATGAT TCAAGAAGCT GACATTGGTG TAGGAATTAG TGGGGTTGAA 
GGAATGCAGG CTGTTATGGC TAGTGACTTT TCGATTGCCC AGTTCCGGTT TCTGGAACGA 
TTACTCGTCG TCCACGGACA CTGGTGCTAC AAAAGGATAG CTCAGATGAT TTGCTATTTC 
TTCTACAAAA ACATAGCATT TGGTCTCACT CTCTTCTACT TTGAGGCTTT CACCGGGTTT 
TCCGGGCAAT CAGTATACAA TGACTACTAC TTGTTGCTCT TCAATGTTGT CCTTACCTCA 
TTGCCAGTAA TCGCCCTTGG TGTCTTTGAA CAGGATGTTT CTTCCGAGAT CTGCTTACAG 
TTCCCAGCTT TATACCAACA AGGCACAAAG AACCTCTTCT TTGATTGGTC AAGAATACTT 
GGATGGATGT GCAATGGCGT CTATGCATCT CTTGTCATAT TCTTCCTCAA CATCGGAATC 
ATTTACTCTC AAGCTTTCAG AGACAATGGC CAAACAGCTG ACATGGATGC TGTTGGCACA 
ACCATGTTCA CTTGCATAAT CTGGGCAGCT AATGTTCAAA TCGCGTTAAC CATGTCCCAT 
TTCACTTGGA TCCAACACGT TTTGATATGG GGAAGCATCG GTATGTGGTA TCTCTTTGTG 
GCTATCTACA GTATGATGCC TCCGAGTTAT TCTGGAAACA TTTACAGGAT TCTTGATGAG 
ATTCTTGCTC CTGCACCTAT CTACTGGATG GCTACATTGT TGGTCACAGT GGCTGCAGTT 
CTTCCTTACG TTGCTCACAT TGCATTCCAG AGATTCTTGA ATCCATTGGA TCATCATATC 
ATCCAAGAGA TCAAATACTA CGGAAGAGAT ATTGAAGATG CGAGATTGTG GACCCGAGAG 
CGTACAAAAG CCCGAGAAAA GACAAAGATC GGATTCACAG CTAGAGTCGA TGCGAAAATC 
AGGCATCTAA GGTCGAAACT GAACAAGAAA CAGTCAAATC TCTCACATTT TTCAGCTCAA 
GACGCAATGT CACCTAGATC ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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AT1G72700 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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