AT1G54280 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT1G54280
Entrez Gene ID	841869
Full Name	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
Synonyms	F20D21.10,F20D21_10
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001333639.1	NP_001319223.1	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
NM_001333640.1	NP_001322413.1	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
NM_104306.3	NP_175830.1	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP8B4	NP_079113.2
Pan troglodytes (chimpanzee)	ATP8B4	XP_001168022.1
Arabidopsis thaliana (thale cress)	ACA.l	NP_172780.1
Arabidopsis thaliana (thale cress)	AT3G13900	NP_188006.4
Arabidopsis thaliana (thale cress)	AT1G68710	NP_177038.1
Mus musculus (house mouse)	Atp8b4	NP_001074413.2
Rattus norvegicus (Norway rat)	Atp8b4	XP_006235000.1
Danio rerio (zebrafish)	si:ch211-167m1.4	XP_687715.4
Arabidopsis thaliana (thale cress)	AT3G27870	NP_189425.2
	ATP8B4	XP_002804819.1
Bos taurus (cattle)	LOC100137953	XP_005211944.1
Arabidopsis thaliana (thale cress)	AT1G26130	NP_001185096.1
Arabidopsis thaliana (thale cress)	AT3G25610	NP_189189.1
Xenopus tropicalis (tropical clawed frog)	atp8b4	XP_002936618.2
Arabidopsis thaliana (thale cress)	AT1G17500	NP_173193.2
Canis lupus familiaris (dog)	ATP8B4	XP_005638457.1
Arabidopsis thaliana (thale cress)	AT1G54280	NP_175830.1
Arabidopsis thaliana (thale cress)	AT1G72700	NP_177414.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT1G54280 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT1G54280 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb26363	NM_001333639.1 Latest version!	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb26363	NM_104306.3 Latest version!	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OAb26363	NM_001333640.1 Latest version!	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT1G54280 ( NM_001333639.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001319223.1 Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.

Price & Availability↑

CloneID	OAb26363
Clone ID Related Accession (Same CDS sequence)	NM_001333639.1 , NM_001333640.1 , NM_104306.3
Accession Version	NM_001333639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCTCGAC GTAGAATAAG ATCAAGGATT CGTAAAAGTC ATTTCTATAC ATTTAGATGT 
CTTAGACCTA AAACTCTTGA TGATCAAGGA CCTCATGTTA TCAATGGACC TGGTTACACC 
AGAATCGTTC ATTGTAACCA GCCTCATTTG CATTTGGCTA CTAAACTCAT TAGGTACCGT 
TCAAACTATG TATCGACCAC CAGGTATAAC TTGCTCACTT TCTTACCTAA ATGCTTGTAC 
GAGCAATTCC ACCGCGTTGC CAATTTCTAT TTCCTCGTTG CTGCCATTCT CTCTGTTTTT 
CCTCTGTCTC CGTTTAACAA ATGGAGCATG ATTGCTCCTT TGGTATTCGT TGTTGGGCTT 
AGTATGGGGA AAGAAGCCTT GGAGGATTGG AGAAGGTTTA TGCAGGACGT TGAGGTGAAC 
TCTAGGAAAG CTAGTGTTCA TAAAGGGAGT GGTGATTTTG GTAGAAGAAC GTGGAAGAGA 
ATCCGTGTTG GTGATATTGT AAGAGTGGAG AAAGATGAGT TTTTTCCTGC TGATTTGCTC 
TTGTTGTCAT CGAGTTACGA GGATGGGATA TGTTATGTTG AGACTATGAA CTTAGATGGT 
GAAACTAACT TGAAAGTTAA GAGATGTTTG GATGCTACTT TGGCATTGGA GAAGGATGAA 
TCTTTTCAGA ACTTCTCTGG AACGATAAAA TGCGAAGATC CGAATCCGAA TCTGTATACG 
TTTGTTGGTA ATCTTGAGTG TGATGGTCAG GTTTATCCAC TTGATCCTAA CCAGATTCTT 
TTGAGAGACT CAAAGCTTAG GAACACTGCT TATGTCTATG GTGTTGTGGT TTTTACTGGC 
CATGATACAA AAGTAATGCA GAATTCTACA AAATCTCCTT CAAAGAGAAG CAGGATTGAA 
AAGAGAATGG ACTACATTAT ATATACTCTC TTTGCACTTC TCTTGACTGT TTCCTTCATC 
AGCTCTCTGG GTTTTGCTGT GATGACAAAA CTTTTAATGG CAGAGTGGTG GTACTTAAGA 
CCAGACAAGC CTGAGAGCTT GACAAATCCT ACAAATCCTT TGTATGCGTG GGTTGTTCAT 
TTGATCACTG CTCTGTTACT TTATGGATAC TTGATTCCCA TCTCATTGTA TGTTTCCATT 
GAGGTAGTGA AAGTCTTGCA AGCACATTTT ATAAATCAAG ACTTGCAGTT GTATGATAGT 
GAGAGTGGGA CTCCGGCTCA AGCACGCACC TCGAATTTAA ATGAAGAGCT GGGACAAGTT 
GATACTATCC TTTCTGATAA AACAGGAACT TTGACATGTA ACCAGATGGA CTTTCTGAAA 
TGCTCTATTG CGGGGACTTC TTACGGTGTC CGTGCTAGTG AAGTGGAACT AGCTGCTGCT 
AAGCAGATGG CAATGGATCT TGAGGAGAAA GGTGAAGAAG TTGCAAACCT TTCTATGAAT 
AAAGGCAGGA CACAACGGTA TGCAAAATTG GCTAGCAAGA CGTCATCAGA TTTTGAATTG 
GAGACAGTAG TCACTGCTAG CGACGAAAAG GATCAGAAGC AGAACACTGG TGTTAAGGGG 
TTTAGTTTTG AAGATAACCG TTTGATGAAT GAAAACTGGT TAAATGAGCC AAATTCAGAT 
GACATTTTGA TGTTTTTCCG TATTCTGGCG GTTTGTCATA CAGCTATTCC TGAGGTGGAT 
GAAGATACTG GAATGTGTAC TTATGAAGCT GAGTCTCCTG ATGAAGTTGC TTTCCTTGTT 
GCTTCTAGGG AGTTTGGTTT TGAATTTACT AAGAGAACTC AATCTAGTGT TTTTATTGCT 
GAACGGTTTT CATCTTCAGG TCAACCAGTT GATAGAGAAT ACAAAATCTT GAATCTGTTA 
GACTTCACTA GCAAAAGAAA AAGAATGTCT GCCATTGTCA GAGATGAAGA AGGGCAGATT 
CTTCTACTAT GTAAAGGCGC TGACAGCATT ATATTTGAAC GTTTATCAAA GAGTGGGAAA 
GAGTATCTTG GAGCTACTTC AAAGCACTTG AATGTATATG GTGAAGCGGG TTTACGCACA 
TTGGCACTTG GTTACAGAAA GCTAGATGAG ACCGAGTATG CAGCATGGAA CAGTGAATTT 
CACAAAGCCA AAACTTCAGT TGGAGCTGAT AGAGATGAAA TGCTCGAGAA GGTTTCAGAT 
ATGATGGAGA AGGAATTGAT CCTTGTTGGA GCTACTGCTG TGGAGGACAA ACTGCAAAAA 
GGGGTGCCTC AATGCATAGA TAACCTTGCC CAAGCTGGTC TCAAAATATG GGTTTTGACA 
GGAGATAAGA TGGAGACAGC AATAAACATA GGATATGCCT GCAGTTTACT ACGACAGGGT 
ATGAAGCAGA TATCCATTTC ACTTACAAAT GTGGAGGAAT CATCACAAAA CTCTGAAGCT 
GCTGCAAAGG AGAGCATTTT GATGCAGATC ACAAATGCTT CACAGATGAT TAAAATTGAA 
AAGGATCCAC ATGCAGCATT TGCTTTGATC ATTGATGGAA AAACACTTAC CTATGCTTTG 
AAGGATGATG TCAAGTATCA GTTTCTTGCC CTTGCAGTTG ACTGTGCATC AGTGATATGC 
TGTCGTGTCT CTCCCAAACA GAAAGCACTT GTAACAAGGC TGGCCAAAGA AGGAACAGGA 
AAAACAACTT TGGCAATTGG TGATGGTGCA AATGATGTTG GAATGATTCA AGAAGCTGAT 
ATTGGTGTTG GCATCAGTGG CGTTGAAGGC ATGCAGGCAG TAATGGCAAG TGACTTCTCC 
ATAGCCCAGT TTCGGTTTCT TGAGAGACTA CTTGTTGTCC ACGGACATTG GTGCTACAAA 
CGAATAGCCC AGATGATTTG TTATTTCTTC TACAAGAACA TAACCTTTGG TCTGACTCTC 
TTCTACTTCG AATGCTTTAC CGGATTTTCT GGTCAATCAA TATACAACGA CTCCTACTTA 
CTACTCTTCA ACGTTGTTCT CACTTCTCTC CCGGTCATTT CCCTCGGAGT TTTTGAACAA 
GATGTTCCTT CTGATGTCTG CTTACAGTTC CCTGCATTGT ATCAACAAGG CCCCAAGAAC 
CTGTTCTTCG ACTGGTACAG AATCCTTGGA TGGATGGGGA ATGGAGTTTA TGCATCCATA 
GTCATCTTTA CACTCAACCT TGGAATCTTC CATGTTCAAT CATTCCGCTC AGATGGCCAA 
ACCGCAGATA TGAATGCCAT GGGAACCGCA ATGTTCACAT GCATCATATG GGCAGTAAAT 
GTTCAAATAG CTCTAACCAT GAGTCACTTC ACTTGGATCC AACACGTAAT GATTTGGGGA 
AGCATAGGAG CTTGGTACGT CTTCCTCGCT CTTTACGGTA TGTTACCAGT AAAACTCTCT 
GGTAACATCT TTCACATGCT CGTCGAGATT CTTGCCCCTG CACCCATCTT CTGGCTTACT 
TCACTTCTGG TCATTGCCGC CACAACGCTA CCTTACTTGT TCCACATTTC ATACCAAAGA 
TCAGTGAACC CTCTTGACCA TCACATTATA CAAGAGATCA AACATTTCAG GATCGATGTA 
GAGGATGAGC GAATGTGGAA ACGAGAGAAG TCAAAGGCGA GAGAGAAGAC AAAGATTGGG 
TTCACGGCTC GAGTTGATGC TAAGATCAGG CAGCTAAGAG GCAGGCTTCA AAGGAAGCAT 
TCGGTGTTAA GTGTTATGAG TGGAACATCG TCTAATGATA CACCTTCTTC AAACTCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001319223.1
CDS	375..4097
Translation	MARRRIRSRIRKSHFYTFRCLRPKTLDDQGPHVINGPGYTRIVHCNQPHLHLATKLIRYRSNYVSTTRYNLLTFLPKCLYEQFHRVANFYFLVAAILSVFPLSPFNKWSMIAPLVFVVGLSMGKEALEDWRRFMQDVEVNSRKASVHKGSGDFGRRTWKRIRVGDIVRVEKDEFFPADLLLLSSSYEDGICYVETMNLDGETNLKVKRCLDATLALEKDESFQNFSGTIKCEDPNPNLYTFVGNLECDGQVYPLDPNQILLRDSKLRNTAYVYGVVVFTGHDTKVMQNSTKSPSKRSRIEKRMDYIIYTLFALLLTVSFISSLGFAVMTKLLMAEWWYLRPDKPESLTNPTNPLYAWVVHLITALLLYGYLIPISLYVSIEVVKVLQAHFINQDLQLYDSESGTPAQARTSNLNEELGQVDTILSDKTGTLTCNQMDFLKCSIAGTSYGVRASEVELAAAKQMAMDLEEKGEEVANLSMNKGRTQRYAKLASKTSSDFELETVVTASDEKDQKQNTGVKGFSFEDNRLMNENWLNEPNSDDILMFFRILAVCHTAIPEVDEDTGMCTYEAESPDEVAFLVASREFGFEFTKRTQSSVFIAERFSSSGQPVDREYKILNLLDFTSKRKRMSAIVRDEEGQILLLCKGADSIIFERLSKSGKEYLGATSKHLNVYGEAGLRTLALGYRKLDETEYAAWNSEFHKAKTSVGADRDEMLEKVSDMMEKELILVGATAVEDKLQKGVPQCIDNLAQAGLKIWVLTGDKMETAINIGYACSLLRQGMKQISISLTNVEESSQNSEAAAKESILMQITNASQMIKIEKDPHAAFALIIDGKTLTYALKDDVKYQFLALAVDCASVICCRVSPKQKALVTRLAKEGTGKTTLAIGDGANDVGMIQEADIGVGISGVEGMQAVMASDFSIAQFRFLERLLVVHGHWCYKRIAQMICYFFYKNITFGLTLFYFECFTGFSGQSIYNDSYLLLFNVVLTSLPVISLGVFEQDVPSDVCLQFPALYQQGPKNLFFDWYRILGWMGNGVYASIVIFTLNLGIFHVQSFRSDGQTADMNAMGTAMFTCIIWAVNVQIALTMSHFTWIQHVMIWGSIGAWYVFLALYGMLPVKLSGNIFHMLVEILAPAPIFWLTSLLVIAATTLPYLFHISYQRSVNPLDHHIIQEIKHFRIDVEDERMWKREKSKAREKTKIGFTARVDAKIRQLRGRLQRKHSVLSVMSGTSSNDTPSSNSQ

Target ORF information:

RefSeq Version	NM_001333639.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333639.1

ORF Insert Sequence:

ATGGCTCGAC GTAGAATAAG ATCAAGGATT CGTAAAAGTC ATTTCTATAC ATTTAGATGT 
CTTAGACCTA AAACTCTTGA TGATCAAGGA CCTCATGTTA TCAATGGACC TGGTTACACC 
AGAATCGTTC ATTGTAACCA GCCTCATTTG CATTTGGCTA CTAAACTCAT TAGGTACCGT 
TCAAACTATG TATCGACCAC CAGGTATAAC TTGCTCACTT TCTTACCTAA ATGCTTGTAC 
GAGCAATTCC ACCGCGTTGC CAATTTCTAT TTCCTCGTTG CTGCCATTCT CTCTGTTTTT 
CCTCTGTCTC CGTTTAACAA ATGGAGCATG ATTGCTCCTT TGGTATTCGT TGTTGGGCTT 
AGTATGGGGA AAGAAGCCTT GGAGGATTGG AGAAGGTTTA TGCAGGACGT TGAGGTGAAC 
TCTAGGAAAG CTAGTGTTCA TAAAGGGAGT GGTGATTTTG GTAGAAGAAC GTGGAAGAGA 
ATCCGTGTTG GTGATATTGT AAGAGTGGAG AAAGATGAGT TTTTTCCTGC TGATTTGCTC 
TTGTTGTCAT CGAGTTACGA GGATGGGATA TGTTATGTTG AGACTATGAA CTTAGATGGT 
GAAACTAACT TGAAAGTTAA GAGATGTTTG GATGCTACTT TGGCATTGGA GAAGGATGAA 
TCTTTTCAGA ACTTCTCTGG AACGATAAAA TGCGAAGATC CGAATCCGAA TCTGTATACG 
TTTGTTGGTA ATCTTGAGTG TGATGGTCAG GTTTATCCAC TTGATCCTAA CCAGATTCTT 
TTGAGAGACT CAAAGCTTAG GAACACTGCT TATGTCTATG GTGTTGTGGT TTTTACTGGC 
CATGATACAA AAGTAATGCA GAATTCTACA AAATCTCCTT CAAAGAGAAG CAGGATTGAA 
AAGAGAATGG ACTACATTAT ATATACTCTC TTTGCACTTC TCTTGACTGT TTCCTTCATC 
AGCTCTCTGG GTTTTGCTGT GATGACAAAA CTTTTAATGG CAGAGTGGTG GTACTTAAGA 
CCAGACAAGC CTGAGAGCTT GACAAATCCT ACAAATCCTT TGTATGCGTG GGTTGTTCAT 
TTGATCACTG CTCTGTTACT TTATGGATAC TTGATTCCCA TCTCATTGTA TGTTTCCATT 
GAGGTAGTGA AAGTCTTGCA AGCACATTTT ATAAATCAAG ACTTGCAGTT GTATGATAGT 
GAGAGTGGGA CTCCGGCTCA AGCACGCACC TCGAATTTAA ATGAAGAGCT GGGACAAGTT 
GATACTATCC TTTCTGATAA AACAGGAACT TTGACATGTA ACCAGATGGA CTTTCTGAAA 
TGCTCTATTG CGGGGACTTC TTACGGTGTC CGTGCTAGTG AAGTGGAACT AGCTGCTGCT 
AAGCAGATGG CAATGGATCT TGAGGAGAAA GGTGAAGAAG TTGCAAACCT TTCTATGAAT 
AAAGGCAGGA CACAACGGTA TGCAAAATTG GCTAGCAAGA CGTCATCAGA TTTTGAATTG 
GAGACAGTAG TCACTGCTAG CGACGAAAAG GATCAGAAGC AGAACACTGG TGTTAAGGGG 
TTTAGTTTTG AAGATAACCG TTTGATGAAT GAAAACTGGT TAAATGAGCC AAATTCAGAT 
GACATTTTGA TGTTTTTCCG TATTCTGGCG GTTTGTCATA CAGCTATTCC TGAGGTGGAT 
GAAGATACTG GAATGTGTAC TTATGAAGCT GAGTCTCCTG ATGAAGTTGC TTTCCTTGTT 
GCTTCTAGGG AGTTTGGTTT TGAATTTACT AAGAGAACTC AATCTAGTGT TTTTATTGCT 
GAACGGTTTT CATCTTCAGG TCAACCAGTT GATAGAGAAT ACAAAATCTT GAATCTGTTA 
GACTTCACTA GCAAAAGAAA AAGAATGTCT GCCATTGTCA GAGATGAAGA AGGGCAGATT 
CTTCTACTAT GTAAAGGCGC TGACAGCATT ATATTTGAAC GTTTATCAAA GAGTGGGAAA 
GAGTATCTTG GAGCTACTTC AAAGCACTTG AATGTATATG GTGAAGCGGG TTTACGCACA 
TTGGCACTTG GTTACAGAAA GCTAGATGAG ACCGAGTATG CAGCATGGAA CAGTGAATTT 
CACAAAGCCA AAACTTCAGT TGGAGCTGAT AGAGATGAAA TGCTCGAGAA GGTTTCAGAT 
ATGATGGAGA AGGAATTGAT CCTTGTTGGA GCTACTGCTG TGGAGGACAA ACTGCAAAAA 
GGGGTGCCTC AATGCATAGA TAACCTTGCC CAAGCTGGTC TCAAAATATG GGTTTTGACA 
GGAGATAAGA TGGAGACAGC AATAAACATA GGATATGCCT GCAGTTTACT ACGACAGGGT 
ATGAAGCAGA TATCCATTTC ACTTACAAAT GTGGAGGAAT CATCACAAAA CTCTGAAGCT 
GCTGCAAAGG AGAGCATTTT GATGCAGATC ACAAATGCTT CACAGATGAT TAAAATTGAA 
AAGGATCCAC ATGCAGCATT TGCTTTGATC ATTGATGGAA AAACACTTAC CTATGCTTTG 
AAGGATGATG TCAAGTATCA GTTTCTTGCC CTTGCAGTTG ACTGTGCATC AGTGATATGC 
TGTCGTGTCT CTCCCAAACA GAAAGCACTT GTAACAAGGC TGGCCAAAGA AGGAACAGGA 
AAAACAACTT TGGCAATTGG TGATGGTGCA AATGATGTTG GAATGATTCA AGAAGCTGAT 
ATTGGTGTTG GCATCAGTGG CGTTGAAGGC ATGCAGGCAG TAATGGCAAG TGACTTCTCC 
ATAGCCCAGT TTCGGTTTCT TGAGAGACTA CTTGTTGTCC ACGGACATTG GTGCTACAAA 
CGAATAGCCC AGATGATTTG TTATTTCTTC TACAAGAACA TAACCTTTGG TCTGACTCTC 
TTCTACTTCG AATGCTTTAC CGGATTTTCT GGTCAATCAA TATACAACGA CTCCTACTTA 
CTACTCTTCA ACGTTGTTCT CACTTCTCTC CCGGTCATTT CCCTCGGAGT TTTTGAACAA 
GATGTTCCTT CTGATGTCTG CTTACAGTTC CCTGCATTGT ATCAACAAGG CCCCAAGAAC 
CTGTTCTTCG ACTGGTACAG AATCCTTGGA TGGATGGGGA ATGGAGTTTA TGCATCCATA 
GTCATCTTTA CACTCAACCT TGGAATCTTC CATGTTCAAT CATTCCGCTC AGATGGCCAA 
ACCGCAGATA TGAATGCCAT GGGAACCGCA ATGTTCACAT GCATCATATG GGCAGTAAAT 
GTTCAAATAG CTCTAACCAT GAGTCACTTC ACTTGGATCC AACACGTAAT GATTTGGGGA 
AGCATAGGAG CTTGGTACGT CTTCCTCGCT CTTTACGGTA TGTTACCAGT AAAACTCTCT 
GGTAACATCT TTCACATGCT CGTCGAGATT CTTGCCCCTG CACCCATCTT CTGGCTTACT 
TCACTTCTGG TCATTGCCGC CACAACGCTA CCTTACTTGT TCCACATTTC ATACCAAAGA 
TCAGTGAACC CTCTTGACCA TCACATTATA CAAGAGATCA AACATTTCAG GATCGATGTA 
GAGGATGAGC GAATGTGGAA ACGAGAGAAG TCAAAGGCGA GAGAGAAGAC AAAGATTGGG 
TTCACGGCTC GAGTTGATGC TAAGATCAGG CAGCTAAGAG GCAGGCTTCA AAGGAAGCAT 
TCGGTGTTAA GTGTTATGAG TGGAACATCG TCTAATGATA CACCTTCTTC AAACTCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT1G54280 ( NM_001333640.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322413.1 Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.

Price & Availability↑

CloneID	OAb26363
Clone ID Related Accession (Same CDS sequence)	NM_001333639.1 , NM_001333640.1 , NM_104306.3
Accession Version	NM_001333640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCTCGAC GTAGAATAAG ATCAAGGATT CGTAAAAGTC ATTTCTATAC ATTTAGATGT 
CTTAGACCTA AAACTCTTGA TGATCAAGGA CCTCATGTTA TCAATGGACC TGGTTACACC 
AGAATCGTTC ATTGTAACCA GCCTCATTTG CATTTGGCTA CTAAACTCAT TAGGTACCGT 
TCAAACTATG TATCGACCAC CAGGTATAAC TTGCTCACTT TCTTACCTAA ATGCTTGTAC 
GAGCAATTCC ACCGCGTTGC CAATTTCTAT TTCCTCGTTG CTGCCATTCT CTCTGTTTTT 
CCTCTGTCTC CGTTTAACAA ATGGAGCATG ATTGCTCCTT TGGTATTCGT TGTTGGGCTT 
AGTATGGGGA AAGAAGCCTT GGAGGATTGG AGAAGGTTTA TGCAGGACGT TGAGGTGAAC 
TCTAGGAAAG CTAGTGTTCA TAAAGGGAGT GGTGATTTTG GTAGAAGAAC GTGGAAGAGA 
ATCCGTGTTG GTGATATTGT AAGAGTGGAG AAAGATGAGT TTTTTCCTGC TGATTTGCTC 
TTGTTGTCAT CGAGTTACGA GGATGGGATA TGTTATGTTG AGACTATGAA CTTAGATGGT 
GAAACTAACT TGAAAGTTAA GAGATGTTTG GATGCTACTT TGGCATTGGA GAAGGATGAA 
TCTTTTCAGA ACTTCTCTGG AACGATAAAA TGCGAAGATC CGAATCCGAA TCTGTATACG 
TTTGTTGGTA ATCTTGAGTG TGATGGTCAG GTTTATCCAC TTGATCCTAA CCAGATTCTT 
TTGAGAGACT CAAAGCTTAG GAACACTGCT TATGTCTATG GTGTTGTGGT TTTTACTGGC 
CATGATACAA AAGTAATGCA GAATTCTACA AAATCTCCTT CAAAGAGAAG CAGGATTGAA 
AAGAGAATGG ACTACATTAT ATATACTCTC TTTGCACTTC TCTTGACTGT TTCCTTCATC 
AGCTCTCTGG GTTTTGCTGT GATGACAAAA CTTTTAATGG CAGAGTGGTG GTACTTAAGA 
CCAGACAAGC CTGAGAGCTT GACAAATCCT ACAAATCCTT TGTATGCGTG GGTTGTTCAT 
TTGATCACTG CTCTGTTACT TTATGGATAC TTGATTCCCA TCTCATTGTA TGTTTCCATT 
GAGGTAGTGA AAGTCTTGCA AGCACATTTT ATAAATCAAG ACTTGCAGTT GTATGATAGT 
GAGAGTGGGA CTCCGGCTCA AGCACGCACC TCGAATTTAA ATGAAGAGCT GGGACAAGTT 
GATACTATCC TTTCTGATAA AACAGGAACT TTGACATGTA ACCAGATGGA CTTTCTGAAA 
TGCTCTATTG CGGGGACTTC TTACGGTGTC CGTGCTAGTG AAGTGGAACT AGCTGCTGCT 
AAGCAGATGG CAATGGATCT TGAGGAGAAA GGTGAAGAAG TTGCAAACCT TTCTATGAAT 
AAAGGCAGGA CACAACGGTA TGCAAAATTG GCTAGCAAGA CGTCATCAGA TTTTGAATTG 
GAGACAGTAG TCACTGCTAG CGACGAAAAG GATCAGAAGC AGAACACTGG TGTTAAGGGG 
TTTAGTTTTG AAGATAACCG TTTGATGAAT GAAAACTGGT TAAATGAGCC AAATTCAGAT 
GACATTTTGA TGTTTTTCCG TATTCTGGCG GTTTGTCATA CAGCTATTCC TGAGGTGGAT 
GAAGATACTG GAATGTGTAC TTATGAAGCT GAGTCTCCTG ATGAAGTTGC TTTCCTTGTT 
GCTTCTAGGG AGTTTGGTTT TGAATTTACT AAGAGAACTC AATCTAGTGT TTTTATTGCT 
GAACGGTTTT CATCTTCAGG TCAACCAGTT GATAGAGAAT ACAAAATCTT GAATCTGTTA 
GACTTCACTA GCAAAAGAAA AAGAATGTCT GCCATTGTCA GAGATGAAGA AGGGCAGATT 
CTTCTACTAT GTAAAGGCGC TGACAGCATT ATATTTGAAC GTTTATCAAA GAGTGGGAAA 
GAGTATCTTG GAGCTACTTC AAAGCACTTG AATGTATATG GTGAAGCGGG TTTACGCACA 
TTGGCACTTG GTTACAGAAA GCTAGATGAG ACCGAGTATG CAGCATGGAA CAGTGAATTT 
CACAAAGCCA AAACTTCAGT TGGAGCTGAT AGAGATGAAA TGCTCGAGAA GGTTTCAGAT 
ATGATGGAGA AGGAATTGAT CCTTGTTGGA GCTACTGCTG TGGAGGACAA ACTGCAAAAA 
GGGGTGCCTC AATGCATAGA TAACCTTGCC CAAGCTGGTC TCAAAATATG GGTTTTGACA 
GGAGATAAGA TGGAGACAGC AATAAACATA GGATATGCCT GCAGTTTACT ACGACAGGGT 
ATGAAGCAGA TATCCATTTC ACTTACAAAT GTGGAGGAAT CATCACAAAA CTCTGAAGCT 
GCTGCAAAGG AGAGCATTTT GATGCAGATC ACAAATGCTT CACAGATGAT TAAAATTGAA 
AAGGATCCAC ATGCAGCATT TGCTTTGATC ATTGATGGAA AAACACTTAC CTATGCTTTG 
AAGGATGATG TCAAGTATCA GTTTCTTGCC CTTGCAGTTG ACTGTGCATC AGTGATATGC 
TGTCGTGTCT CTCCCAAACA GAAAGCACTT GTAACAAGGC TGGCCAAAGA AGGAACAGGA 
AAAACAACTT TGGCAATTGG TGATGGTGCA AATGATGTTG GAATGATTCA AGAAGCTGAT 
ATTGGTGTTG GCATCAGTGG CGTTGAAGGC ATGCAGGCAG TAATGGCAAG TGACTTCTCC 
ATAGCCCAGT TTCGGTTTCT TGAGAGACTA CTTGTTGTCC ACGGACATTG GTGCTACAAA 
CGAATAGCCC AGATGATTTG TTATTTCTTC TACAAGAACA TAACCTTTGG TCTGACTCTC 
TTCTACTTCG AATGCTTTAC CGGATTTTCT GGTCAATCAA TATACAACGA CTCCTACTTA 
CTACTCTTCA ACGTTGTTCT CACTTCTCTC CCGGTCATTT CCCTCGGAGT TTTTGAACAA 
GATGTTCCTT CTGATGTCTG CTTACAGTTC CCTGCATTGT ATCAACAAGG CCCCAAGAAC 
CTGTTCTTCG ACTGGTACAG AATCCTTGGA TGGATGGGGA ATGGAGTTTA TGCATCCATA 
GTCATCTTTA CACTCAACCT TGGAATCTTC CATGTTCAAT CATTCCGCTC AGATGGCCAA 
ACCGCAGATA TGAATGCCAT GGGAACCGCA ATGTTCACAT GCATCATATG GGCAGTAAAT 
GTTCAAATAG CTCTAACCAT GAGTCACTTC ACTTGGATCC AACACGTAAT GATTTGGGGA 
AGCATAGGAG CTTGGTACGT CTTCCTCGCT CTTTACGGTA TGTTACCAGT AAAACTCTCT 
GGTAACATCT TTCACATGCT CGTCGAGATT CTTGCCCCTG CACCCATCTT CTGGCTTACT 
TCACTTCTGG TCATTGCCGC CACAACGCTA CCTTACTTGT TCCACATTTC ATACCAAAGA 
TCAGTGAACC CTCTTGACCA TCACATTATA CAAGAGATCA AACATTTCAG GATCGATGTA 
GAGGATGAGC GAATGTGGAA ACGAGAGAAG TCAAAGGCGA GAGAGAAGAC AAAGATTGGG 
TTCACGGCTC GAGTTGATGC TAAGATCAGG CAGCTAAGAG GCAGGCTTCA AAGGAAGCAT 
TCGGTGTTAA GTGTTATGAG TGGAACATCG TCTAATGATA CACCTTCTTC AAACTCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322413.1
CDS	285..4007
Translation	MARRRIRSRIRKSHFYTFRCLRPKTLDDQGPHVINGPGYTRIVHCNQPHLHLATKLIRYRSNYVSTTRYNLLTFLPKCLYEQFHRVANFYFLVAAILSVFPLSPFNKWSMIAPLVFVVGLSMGKEALEDWRRFMQDVEVNSRKASVHKGSGDFGRRTWKRIRVGDIVRVEKDEFFPADLLLLSSSYEDGICYVETMNLDGETNLKVKRCLDATLALEKDESFQNFSGTIKCEDPNPNLYTFVGNLECDGQVYPLDPNQILLRDSKLRNTAYVYGVVVFTGHDTKVMQNSTKSPSKRSRIEKRMDYIIYTLFALLLTVSFISSLGFAVMTKLLMAEWWYLRPDKPESLTNPTNPLYAWVVHLITALLLYGYLIPISLYVSIEVVKVLQAHFINQDLQLYDSESGTPAQARTSNLNEELGQVDTILSDKTGTLTCNQMDFLKCSIAGTSYGVRASEVELAAAKQMAMDLEEKGEEVANLSMNKGRTQRYAKLASKTSSDFELETVVTASDEKDQKQNTGVKGFSFEDNRLMNENWLNEPNSDDILMFFRILAVCHTAIPEVDEDTGMCTYEAESPDEVAFLVASREFGFEFTKRTQSSVFIAERFSSSGQPVDREYKILNLLDFTSKRKRMSAIVRDEEGQILLLCKGADSIIFERLSKSGKEYLGATSKHLNVYGEAGLRTLALGYRKLDETEYAAWNSEFHKAKTSVGADRDEMLEKVSDMMEKELILVGATAVEDKLQKGVPQCIDNLAQAGLKIWVLTGDKMETAINIGYACSLLRQGMKQISISLTNVEESSQNSEAAAKESILMQITNASQMIKIEKDPHAAFALIIDGKTLTYALKDDVKYQFLALAVDCASVICCRVSPKQKALVTRLAKEGTGKTTLAIGDGANDVGMIQEADIGVGISGVEGMQAVMASDFSIAQFRFLERLLVVHGHWCYKRIAQMICYFFYKNITFGLTLFYFECFTGFSGQSIYNDSYLLLFNVVLTSLPVISLGVFEQDVPSDVCLQFPALYQQGPKNLFFDWYRILGWMGNGVYASIVIFTLNLGIFHVQSFRSDGQTADMNAMGTAMFTCIIWAVNVQIALTMSHFTWIQHVMIWGSIGAWYVFLALYGMLPVKLSGNIFHMLVEILAPAPIFWLTSLLVIAATTLPYLFHISYQRSVNPLDHHIIQEIKHFRIDVEDERMWKREKSKAREKTKIGFTARVDAKIRQLRGRLQRKHSVLSVMSGTSSNDTPSSNSQ

Target ORF information:

RefSeq Version	NM_001333640.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333640.1

ORF Insert Sequence:

ATGGCTCGAC GTAGAATAAG ATCAAGGATT CGTAAAAGTC ATTTCTATAC ATTTAGATGT 
CTTAGACCTA AAACTCTTGA TGATCAAGGA CCTCATGTTA TCAATGGACC TGGTTACACC 
AGAATCGTTC ATTGTAACCA GCCTCATTTG CATTTGGCTA CTAAACTCAT TAGGTACCGT 
TCAAACTATG TATCGACCAC CAGGTATAAC TTGCTCACTT TCTTACCTAA ATGCTTGTAC 
GAGCAATTCC ACCGCGTTGC CAATTTCTAT TTCCTCGTTG CTGCCATTCT CTCTGTTTTT 
CCTCTGTCTC CGTTTAACAA ATGGAGCATG ATTGCTCCTT TGGTATTCGT TGTTGGGCTT 
AGTATGGGGA AAGAAGCCTT GGAGGATTGG AGAAGGTTTA TGCAGGACGT TGAGGTGAAC 
TCTAGGAAAG CTAGTGTTCA TAAAGGGAGT GGTGATTTTG GTAGAAGAAC GTGGAAGAGA 
ATCCGTGTTG GTGATATTGT AAGAGTGGAG AAAGATGAGT TTTTTCCTGC TGATTTGCTC 
TTGTTGTCAT CGAGTTACGA GGATGGGATA TGTTATGTTG AGACTATGAA CTTAGATGGT 
GAAACTAACT TGAAAGTTAA GAGATGTTTG GATGCTACTT TGGCATTGGA GAAGGATGAA 
TCTTTTCAGA ACTTCTCTGG AACGATAAAA TGCGAAGATC CGAATCCGAA TCTGTATACG 
TTTGTTGGTA ATCTTGAGTG TGATGGTCAG GTTTATCCAC TTGATCCTAA CCAGATTCTT 
TTGAGAGACT CAAAGCTTAG GAACACTGCT TATGTCTATG GTGTTGTGGT TTTTACTGGC 
CATGATACAA AAGTAATGCA GAATTCTACA AAATCTCCTT CAAAGAGAAG CAGGATTGAA 
AAGAGAATGG ACTACATTAT ATATACTCTC TTTGCACTTC TCTTGACTGT TTCCTTCATC 
AGCTCTCTGG GTTTTGCTGT GATGACAAAA CTTTTAATGG CAGAGTGGTG GTACTTAAGA 
CCAGACAAGC CTGAGAGCTT GACAAATCCT ACAAATCCTT TGTATGCGTG GGTTGTTCAT 
TTGATCACTG CTCTGTTACT TTATGGATAC TTGATTCCCA TCTCATTGTA TGTTTCCATT 
GAGGTAGTGA AAGTCTTGCA AGCACATTTT ATAAATCAAG ACTTGCAGTT GTATGATAGT 
GAGAGTGGGA CTCCGGCTCA AGCACGCACC TCGAATTTAA ATGAAGAGCT GGGACAAGTT 
GATACTATCC TTTCTGATAA AACAGGAACT TTGACATGTA ACCAGATGGA CTTTCTGAAA 
TGCTCTATTG CGGGGACTTC TTACGGTGTC CGTGCTAGTG AAGTGGAACT AGCTGCTGCT 
AAGCAGATGG CAATGGATCT TGAGGAGAAA GGTGAAGAAG TTGCAAACCT TTCTATGAAT 
AAAGGCAGGA CACAACGGTA TGCAAAATTG GCTAGCAAGA CGTCATCAGA TTTTGAATTG 
GAGACAGTAG TCACTGCTAG CGACGAAAAG GATCAGAAGC AGAACACTGG TGTTAAGGGG 
TTTAGTTTTG AAGATAACCG TTTGATGAAT GAAAACTGGT TAAATGAGCC AAATTCAGAT 
GACATTTTGA TGTTTTTCCG TATTCTGGCG GTTTGTCATA CAGCTATTCC TGAGGTGGAT 
GAAGATACTG GAATGTGTAC TTATGAAGCT GAGTCTCCTG ATGAAGTTGC TTTCCTTGTT 
GCTTCTAGGG AGTTTGGTTT TGAATTTACT AAGAGAACTC AATCTAGTGT TTTTATTGCT 
GAACGGTTTT CATCTTCAGG TCAACCAGTT GATAGAGAAT ACAAAATCTT GAATCTGTTA 
GACTTCACTA GCAAAAGAAA AAGAATGTCT GCCATTGTCA GAGATGAAGA AGGGCAGATT 
CTTCTACTAT GTAAAGGCGC TGACAGCATT ATATTTGAAC GTTTATCAAA GAGTGGGAAA 
GAGTATCTTG GAGCTACTTC AAAGCACTTG AATGTATATG GTGAAGCGGG TTTACGCACA 
TTGGCACTTG GTTACAGAAA GCTAGATGAG ACCGAGTATG CAGCATGGAA CAGTGAATTT 
CACAAAGCCA AAACTTCAGT TGGAGCTGAT AGAGATGAAA TGCTCGAGAA GGTTTCAGAT 
ATGATGGAGA AGGAATTGAT CCTTGTTGGA GCTACTGCTG TGGAGGACAA ACTGCAAAAA 
GGGGTGCCTC AATGCATAGA TAACCTTGCC CAAGCTGGTC TCAAAATATG GGTTTTGACA 
GGAGATAAGA TGGAGACAGC AATAAACATA GGATATGCCT GCAGTTTACT ACGACAGGGT 
ATGAAGCAGA TATCCATTTC ACTTACAAAT GTGGAGGAAT CATCACAAAA CTCTGAAGCT 
GCTGCAAAGG AGAGCATTTT GATGCAGATC ACAAATGCTT CACAGATGAT TAAAATTGAA 
AAGGATCCAC ATGCAGCATT TGCTTTGATC ATTGATGGAA AAACACTTAC CTATGCTTTG 
AAGGATGATG TCAAGTATCA GTTTCTTGCC CTTGCAGTTG ACTGTGCATC AGTGATATGC 
TGTCGTGTCT CTCCCAAACA GAAAGCACTT GTAACAAGGC TGGCCAAAGA AGGAACAGGA 
AAAACAACTT TGGCAATTGG TGATGGTGCA AATGATGTTG GAATGATTCA AGAAGCTGAT 
ATTGGTGTTG GCATCAGTGG CGTTGAAGGC ATGCAGGCAG TAATGGCAAG TGACTTCTCC 
ATAGCCCAGT TTCGGTTTCT TGAGAGACTA CTTGTTGTCC ACGGACATTG GTGCTACAAA 
CGAATAGCCC AGATGATTTG TTATTTCTTC TACAAGAACA TAACCTTTGG TCTGACTCTC 
TTCTACTTCG AATGCTTTAC CGGATTTTCT GGTCAATCAA TATACAACGA CTCCTACTTA 
CTACTCTTCA ACGTTGTTCT CACTTCTCTC CCGGTCATTT CCCTCGGAGT TTTTGAACAA 
GATGTTCCTT CTGATGTCTG CTTACAGTTC CCTGCATTGT ATCAACAAGG CCCCAAGAAC 
CTGTTCTTCG ACTGGTACAG AATCCTTGGA TGGATGGGGA ATGGAGTTTA TGCATCCATA 
GTCATCTTTA CACTCAACCT TGGAATCTTC CATGTTCAAT CATTCCGCTC AGATGGCCAA 
ACCGCAGATA TGAATGCCAT GGGAACCGCA ATGTTCACAT GCATCATATG GGCAGTAAAT 
GTTCAAATAG CTCTAACCAT GAGTCACTTC ACTTGGATCC AACACGTAAT GATTTGGGGA 
AGCATAGGAG CTTGGTACGT CTTCCTCGCT CTTTACGGTA TGTTACCAGT AAAACTCTCT 
GGTAACATCT TTCACATGCT CGTCGAGATT CTTGCCCCTG CACCCATCTT CTGGCTTACT 
TCACTTCTGG TCATTGCCGC CACAACGCTA CCTTACTTGT TCCACATTTC ATACCAAAGA 
TCAGTGAACC CTCTTGACCA TCACATTATA CAAGAGATCA AACATTTCAG GATCGATGTA 
GAGGATGAGC GAATGTGGAA ACGAGAGAAG TCAAAGGCGA GAGAGAAGAC AAAGATTGGG 
TTCACGGCTC GAGTTGATGC TAAGATCAGG CAGCTAAGAG GCAGGCTTCA AAGGAAGCAT 
TCGGTGTTAA GTGTTATGAG TGGAACATCG TCTAATGATA CACCTTCTTC AAACTCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT1G54280 ( NM_104306.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_175830.1 Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.

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CloneID	OAb26363
Clone ID Related Accession (Same CDS sequence)	NM_001333639.1 , NM_001333640.1 , NM_104306.3
Accession Version	NM_104306.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_104306.2.

ATGGCTCGAC GTAGAATAAG ATCAAGGATT CGTAAAAGTC ATTTCTATAC ATTTAGATGT 
CTTAGACCTA AAACTCTTGA TGATCAAGGA CCTCATGTTA TCAATGGACC TGGTTACACC 
AGAATCGTTC ATTGTAACCA GCCTCATTTG CATTTGGCTA CTAAACTCAT TAGGTACCGT 
TCAAACTATG TATCGACCAC CAGGTATAAC TTGCTCACTT TCTTACCTAA ATGCTTGTAC 
GAGCAATTCC ACCGCGTTGC CAATTTCTAT TTCCTCGTTG CTGCCATTCT CTCTGTTTTT 
CCTCTGTCTC CGTTTAACAA ATGGAGCATG ATTGCTCCTT TGGTATTCGT TGTTGGGCTT 
AGTATGGGGA AAGAAGCCTT GGAGGATTGG AGAAGGTTTA TGCAGGACGT TGAGGTGAAC 
TCTAGGAAAG CTAGTGTTCA TAAAGGGAGT GGTGATTTTG GTAGAAGAAC GTGGAAGAGA 
ATCCGTGTTG GTGATATTGT AAGAGTGGAG AAAGATGAGT TTTTTCCTGC TGATTTGCTC 
TTGTTGTCAT CGAGTTACGA GGATGGGATA TGTTATGTTG AGACTATGAA CTTAGATGGT 
GAAACTAACT TGAAAGTTAA GAGATGTTTG GATGCTACTT TGGCATTGGA GAAGGATGAA 
TCTTTTCAGA ACTTCTCTGG AACGATAAAA TGCGAAGATC CGAATCCGAA TCTGTATACG 
TTTGTTGGTA ATCTTGAGTG TGATGGTCAG GTTTATCCAC TTGATCCTAA CCAGATTCTT 
TTGAGAGACT CAAAGCTTAG GAACACTGCT TATGTCTATG GTGTTGTGGT TTTTACTGGC 
CATGATACAA AAGTAATGCA GAATTCTACA AAATCTCCTT CAAAGAGAAG CAGGATTGAA 
AAGAGAATGG ACTACATTAT ATATACTCTC TTTGCACTTC TCTTGACTGT TTCCTTCATC 
AGCTCTCTGG GTTTTGCTGT GATGACAAAA CTTTTAATGG CAGAGTGGTG GTACTTAAGA 
CCAGACAAGC CTGAGAGCTT GACAAATCCT ACAAATCCTT TGTATGCGTG GGTTGTTCAT 
TTGATCACTG CTCTGTTACT TTATGGATAC TTGATTCCCA TCTCATTGTA TGTTTCCATT 
GAGGTAGTGA AAGTCTTGCA AGCACATTTT ATAAATCAAG ACTTGCAGTT GTATGATAGT 
GAGAGTGGGA CTCCGGCTCA AGCACGCACC TCGAATTTAA ATGAAGAGCT GGGACAAGTT 
GATACTATCC TTTCTGATAA AACAGGAACT TTGACATGTA ACCAGATGGA CTTTCTGAAA 
TGCTCTATTG CGGGGACTTC TTACGGTGTC CGTGCTAGTG AAGTGGAACT AGCTGCTGCT 
AAGCAGATGG CAATGGATCT TGAGGAGAAA GGTGAAGAAG TTGCAAACCT TTCTATGAAT 
AAAGGCAGGA CACAACGGTA TGCAAAATTG GCTAGCAAGA CGTCATCAGA TTTTGAATTG 
GAGACAGTAG TCACTGCTAG CGACGAAAAG GATCAGAAGC AGAACACTGG TGTTAAGGGG 
TTTAGTTTTG AAGATAACCG TTTGATGAAT GAAAACTGGT TAAATGAGCC AAATTCAGAT 
GACATTTTGA TGTTTTTCCG TATTCTGGCG GTTTGTCATA CAGCTATTCC TGAGGTGGAT 
GAAGATACTG GAATGTGTAC TTATGAAGCT GAGTCTCCTG ATGAAGTTGC TTTCCTTGTT 
GCTTCTAGGG AGTTTGGTTT TGAATTTACT AAGAGAACTC AATCTAGTGT TTTTATTGCT 
GAACGGTTTT CATCTTCAGG TCAACCAGTT GATAGAGAAT ACAAAATCTT GAATCTGTTA 
GACTTCACTA GCAAAAGAAA AAGAATGTCT GCCATTGTCA GAGATGAAGA AGGGCAGATT 
CTTCTACTAT GTAAAGGCGC TGACAGCATT ATATTTGAAC GTTTATCAAA GAGTGGGAAA 
GAGTATCTTG GAGCTACTTC AAAGCACTTG AATGTATATG GTGAAGCGGG TTTACGCACA 
TTGGCACTTG GTTACAGAAA GCTAGATGAG ACCGAGTATG CAGCATGGAA CAGTGAATTT 
CACAAAGCCA AAACTTCAGT TGGAGCTGAT AGAGATGAAA TGCTCGAGAA GGTTTCAGAT 
ATGATGGAGA AGGAATTGAT CCTTGTTGGA GCTACTGCTG TGGAGGACAA ACTGCAAAAA 
GGGGTGCCTC AATGCATAGA TAACCTTGCC CAAGCTGGTC TCAAAATATG GGTTTTGACA 
GGAGATAAGA TGGAGACAGC AATAAACATA GGATATGCCT GCAGTTTACT ACGACAGGGT 
ATGAAGCAGA TATCCATTTC ACTTACAAAT GTGGAGGAAT CATCACAAAA CTCTGAAGCT 
GCTGCAAAGG AGAGCATTTT GATGCAGATC ACAAATGCTT CACAGATGAT TAAAATTGAA 
AAGGATCCAC ATGCAGCATT TGCTTTGATC ATTGATGGAA AAACACTTAC CTATGCTTTG 
AAGGATGATG TCAAGTATCA GTTTCTTGCC CTTGCAGTTG ACTGTGCATC AGTGATATGC 
TGTCGTGTCT CTCCCAAACA GAAAGCACTT GTAACAAGGC TGGCCAAAGA AGGAACAGGA 
AAAACAACTT TGGCAATTGG TGATGGTGCA AATGATGTTG GAATGATTCA AGAAGCTGAT 
ATTGGTGTTG GCATCAGTGG CGTTGAAGGC ATGCAGGCAG TAATGGCAAG TGACTTCTCC 
ATAGCCCAGT TTCGGTTTCT TGAGAGACTA CTTGTTGTCC ACGGACATTG GTGCTACAAA 
CGAATAGCCC AGATGATTTG TTATTTCTTC TACAAGAACA TAACCTTTGG TCTGACTCTC 
TTCTACTTCG AATGCTTTAC CGGATTTTCT GGTCAATCAA TATACAACGA CTCCTACTTA 
CTACTCTTCA ACGTTGTTCT CACTTCTCTC CCGGTCATTT CCCTCGGAGT TTTTGAACAA 
GATGTTCCTT CTGATGTCTG CTTACAGTTC CCTGCATTGT ATCAACAAGG CCCCAAGAAC 
CTGTTCTTCG ACTGGTACAG AATCCTTGGA TGGATGGGGA ATGGAGTTTA TGCATCCATA 
GTCATCTTTA CACTCAACCT TGGAATCTTC CATGTTCAAT CATTCCGCTC AGATGGCCAA 
ACCGCAGATA TGAATGCCAT GGGAACCGCA ATGTTCACAT GCATCATATG GGCAGTAAAT 
GTTCAAATAG CTCTAACCAT GAGTCACTTC ACTTGGATCC AACACGTAAT GATTTGGGGA 
AGCATAGGAG CTTGGTACGT CTTCCTCGCT CTTTACGGTA TGTTACCAGT AAAACTCTCT 
GGTAACATCT TTCACATGCT CGTCGAGATT CTTGCCCCTG CACCCATCTT CTGGCTTACT 
TCACTTCTGG TCATTGCCGC CACAACGCTA CCTTACTTGT TCCACATTTC ATACCAAAGA 
TCAGTGAACC CTCTTGACCA TCACATTATA CAAGAGATCA AACATTTCAG GATCGATGTA 
GAGGATGAGC GAATGTGGAA ACGAGAGAAG TCAAAGGCGA GAGAGAAGAC AAAGATTGGG 
TTCACGGCTC GAGTTGATGC TAAGATCAGG CAGCTAAGAG GCAGGCTTCA AAGGAAGCAT 
TCGGTGTTAA GTGTTATGAG TGGAACATCG TCTAATGATA CACCTTCTTC AAACTCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_175830.1
CDS	477..4199
Translation	MARRRIRSRIRKSHFYTFRCLRPKTLDDQGPHVINGPGYTRIVHCNQPHLHLATKLIRYRSNYVSTTRYNLLTFLPKCLYEQFHRVANFYFLVAAILSVFPLSPFNKWSMIAPLVFVVGLSMGKEALEDWRRFMQDVEVNSRKASVHKGSGDFGRRTWKRIRVGDIVRVEKDEFFPADLLLLSSSYEDGICYVETMNLDGETNLKVKRCLDATLALEKDESFQNFSGTIKCEDPNPNLYTFVGNLECDGQVYPLDPNQILLRDSKLRNTAYVYGVVVFTGHDTKVMQNSTKSPSKRSRIEKRMDYIIYTLFALLLTVSFISSLGFAVMTKLLMAEWWYLRPDKPESLTNPTNPLYAWVVHLITALLLYGYLIPISLYVSIEVVKVLQAHFINQDLQLYDSESGTPAQARTSNLNEELGQVDTILSDKTGTLTCNQMDFLKCSIAGTSYGVRASEVELAAAKQMAMDLEEKGEEVANLSMNKGRTQRYAKLASKTSSDFELETVVTASDEKDQKQNTGVKGFSFEDNRLMNENWLNEPNSDDILMFFRILAVCHTAIPEVDEDTGMCTYEAESPDEVAFLVASREFGFEFTKRTQSSVFIAERFSSSGQPVDREYKILNLLDFTSKRKRMSAIVRDEEGQILLLCKGADSIIFERLSKSGKEYLGATSKHLNVYGEAGLRTLALGYRKLDETEYAAWNSEFHKAKTSVGADRDEMLEKVSDMMEKELILVGATAVEDKLQKGVPQCIDNLAQAGLKIWVLTGDKMETAINIGYACSLLRQGMKQISISLTNVEESSQNSEAAAKESILMQITNASQMIKIEKDPHAAFALIIDGKTLTYALKDDVKYQFLALAVDCASVICCRVSPKQKALVTRLAKEGTGKTTLAIGDGANDVGMIQEADIGVGISGVEGMQAVMASDFSIAQFRFLERLLVVHGHWCYKRIAQMICYFFYKNITFGLTLFYFECFTGFSGQSIYNDSYLLLFNVVLTSLPVISLGVFEQDVPSDVCLQFPALYQQGPKNLFFDWYRILGWMGNGVYASIVIFTLNLGIFHVQSFRSDGQTADMNAMGTAMFTCIIWAVNVQIALTMSHFTWIQHVMIWGSIGAWYVFLALYGMLPVKLSGNIFHMLVEILAPAPIFWLTSLLVIAATTLPYLFHISYQRSVNPLDHHIIQEIKHFRIDVEDERMWKREKSKAREKTKIGFTARVDAKIRQLRGRLQRKHSVLSVMSGTSSNDTPSSNSQ

Target ORF information:

RefSeq Version	NM_104306.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein (AT1G54280), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_104306.3

ORF Insert Sequence:

ATGGCTCGAC GTAGAATAAG ATCAAGGATT CGTAAAAGTC ATTTCTATAC ATTTAGATGT 
CTTAGACCTA AAACTCTTGA TGATCAAGGA CCTCATGTTA TCAATGGACC TGGTTACACC 
AGAATCGTTC ATTGTAACCA GCCTCATTTG CATTTGGCTA CTAAACTCAT TAGGTACCGT 
TCAAACTATG TATCGACCAC CAGGTATAAC TTGCTCACTT TCTTACCTAA ATGCTTGTAC 
GAGCAATTCC ACCGCGTTGC CAATTTCTAT TTCCTCGTTG CTGCCATTCT CTCTGTTTTT 
CCTCTGTCTC CGTTTAACAA ATGGAGCATG ATTGCTCCTT TGGTATTCGT TGTTGGGCTT 
AGTATGGGGA AAGAAGCCTT GGAGGATTGG AGAAGGTTTA TGCAGGACGT TGAGGTGAAC 
TCTAGGAAAG CTAGTGTTCA TAAAGGGAGT GGTGATTTTG GTAGAAGAAC GTGGAAGAGA 
ATCCGTGTTG GTGATATTGT AAGAGTGGAG AAAGATGAGT TTTTTCCTGC TGATTTGCTC 
TTGTTGTCAT CGAGTTACGA GGATGGGATA TGTTATGTTG AGACTATGAA CTTAGATGGT 
GAAACTAACT TGAAAGTTAA GAGATGTTTG GATGCTACTT TGGCATTGGA GAAGGATGAA 
TCTTTTCAGA ACTTCTCTGG AACGATAAAA TGCGAAGATC CGAATCCGAA TCTGTATACG 
TTTGTTGGTA ATCTTGAGTG TGATGGTCAG GTTTATCCAC TTGATCCTAA CCAGATTCTT 
TTGAGAGACT CAAAGCTTAG GAACACTGCT TATGTCTATG GTGTTGTGGT TTTTACTGGC 
CATGATACAA AAGTAATGCA GAATTCTACA AAATCTCCTT CAAAGAGAAG CAGGATTGAA 
AAGAGAATGG ACTACATTAT ATATACTCTC TTTGCACTTC TCTTGACTGT TTCCTTCATC 
AGCTCTCTGG GTTTTGCTGT GATGACAAAA CTTTTAATGG CAGAGTGGTG GTACTTAAGA 
CCAGACAAGC CTGAGAGCTT GACAAATCCT ACAAATCCTT TGTATGCGTG GGTTGTTCAT 
TTGATCACTG CTCTGTTACT TTATGGATAC TTGATTCCCA TCTCATTGTA TGTTTCCATT 
GAGGTAGTGA AAGTCTTGCA AGCACATTTT ATAAATCAAG ACTTGCAGTT GTATGATAGT 
GAGAGTGGGA CTCCGGCTCA AGCACGCACC TCGAATTTAA ATGAAGAGCT GGGACAAGTT 
GATACTATCC TTTCTGATAA AACAGGAACT TTGACATGTA ACCAGATGGA CTTTCTGAAA 
TGCTCTATTG CGGGGACTTC TTACGGTGTC CGTGCTAGTG AAGTGGAACT AGCTGCTGCT 
AAGCAGATGG CAATGGATCT TGAGGAGAAA GGTGAAGAAG TTGCAAACCT TTCTATGAAT 
AAAGGCAGGA CACAACGGTA TGCAAAATTG GCTAGCAAGA CGTCATCAGA TTTTGAATTG 
GAGACAGTAG TCACTGCTAG CGACGAAAAG GATCAGAAGC AGAACACTGG TGTTAAGGGG 
TTTAGTTTTG AAGATAACCG TTTGATGAAT GAAAACTGGT TAAATGAGCC AAATTCAGAT 
GACATTTTGA TGTTTTTCCG TATTCTGGCG GTTTGTCATA CAGCTATTCC TGAGGTGGAT 
GAAGATACTG GAATGTGTAC TTATGAAGCT GAGTCTCCTG ATGAAGTTGC TTTCCTTGTT 
GCTTCTAGGG AGTTTGGTTT TGAATTTACT AAGAGAACTC AATCTAGTGT TTTTATTGCT 
GAACGGTTTT CATCTTCAGG TCAACCAGTT GATAGAGAAT ACAAAATCTT GAATCTGTTA 
GACTTCACTA GCAAAAGAAA AAGAATGTCT GCCATTGTCA GAGATGAAGA AGGGCAGATT 
CTTCTACTAT GTAAAGGCGC TGACAGCATT ATATTTGAAC GTTTATCAAA GAGTGGGAAA 
GAGTATCTTG GAGCTACTTC AAAGCACTTG AATGTATATG GTGAAGCGGG TTTACGCACA 
TTGGCACTTG GTTACAGAAA GCTAGATGAG ACCGAGTATG CAGCATGGAA CAGTGAATTT 
CACAAAGCCA AAACTTCAGT TGGAGCTGAT AGAGATGAAA TGCTCGAGAA GGTTTCAGAT 
ATGATGGAGA AGGAATTGAT CCTTGTTGGA GCTACTGCTG TGGAGGACAA ACTGCAAAAA 
GGGGTGCCTC AATGCATAGA TAACCTTGCC CAAGCTGGTC TCAAAATATG GGTTTTGACA 
GGAGATAAGA TGGAGACAGC AATAAACATA GGATATGCCT GCAGTTTACT ACGACAGGGT 
ATGAAGCAGA TATCCATTTC ACTTACAAAT GTGGAGGAAT CATCACAAAA CTCTGAAGCT 
GCTGCAAAGG AGAGCATTTT GATGCAGATC ACAAATGCTT CACAGATGAT TAAAATTGAA 
AAGGATCCAC ATGCAGCATT TGCTTTGATC ATTGATGGAA AAACACTTAC CTATGCTTTG 
AAGGATGATG TCAAGTATCA GTTTCTTGCC CTTGCAGTTG ACTGTGCATC AGTGATATGC 
TGTCGTGTCT CTCCCAAACA GAAAGCACTT GTAACAAGGC TGGCCAAAGA AGGAACAGGA 
AAAACAACTT TGGCAATTGG TGATGGTGCA AATGATGTTG GAATGATTCA AGAAGCTGAT 
ATTGGTGTTG GCATCAGTGG CGTTGAAGGC ATGCAGGCAG TAATGGCAAG TGACTTCTCC 
ATAGCCCAGT TTCGGTTTCT TGAGAGACTA CTTGTTGTCC ACGGACATTG GTGCTACAAA 
CGAATAGCCC AGATGATTTG TTATTTCTTC TACAAGAACA TAACCTTTGG TCTGACTCTC 
TTCTACTTCG AATGCTTTAC CGGATTTTCT GGTCAATCAA TATACAACGA CTCCTACTTA 
CTACTCTTCA ACGTTGTTCT CACTTCTCTC CCGGTCATTT CCCTCGGAGT TTTTGAACAA 
GATGTTCCTT CTGATGTCTG CTTACAGTTC CCTGCATTGT ATCAACAAGG CCCCAAGAAC 
CTGTTCTTCG ACTGGTACAG AATCCTTGGA TGGATGGGGA ATGGAGTTTA TGCATCCATA 
GTCATCTTTA CACTCAACCT TGGAATCTTC CATGTTCAAT CATTCCGCTC AGATGGCCAA 
ACCGCAGATA TGAATGCCAT GGGAACCGCA ATGTTCACAT GCATCATATG GGCAGTAAAT 
GTTCAAATAG CTCTAACCAT GAGTCACTTC ACTTGGATCC AACACGTAAT GATTTGGGGA 
AGCATAGGAG CTTGGTACGT CTTCCTCGCT CTTTACGGTA TGTTACCAGT AAAACTCTCT 
GGTAACATCT TTCACATGCT CGTCGAGATT CTTGCCCCTG CACCCATCTT CTGGCTTACT 
TCACTTCTGG TCATTGCCGC CACAACGCTA CCTTACTTGT TCCACATTTC ATACCAAAGA 
TCAGTGAACC CTCTTGACCA TCACATTATA CAAGAGATCA AACATTTCAG GATCGATGTA 
GAGGATGAGC GAATGTGGAA ACGAGAGAAG TCAAAGGCGA GAGAGAAGAC AAAGATTGGG 
TTCACGGCTC GAGTTGATGC TAAGATCAGG CAGCTAAGAG GCAGGCTTCA AAGGAAGCAT 
TCGGTGTTAA GTGTTATGAG TGGAACATCG TCTAATGATA CACCTTCTTC AAACTCACAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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AT1G54280 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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