AT1G45332 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT1G45332
Entrez Gene ID	841097
Full Name	Translation elongation factor EFG/EF2 protein
Synonyms	AT1G45261,F2G19.2,F2G19_2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001333262.1	NP_001321287.1	Translation elongation factor EFG/EF2 protein
NM_103595.4	NP_175135.1	Translation elongation factor EFG/EF2 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GFM1	NP_079272.4
Bos taurus (cattle)	GFM1	NP_001193078.1
Gallus gallus (chicken)	GFM1	XP_001233639.1
Saccharomyces cerevisiae (baker's yeast)	MEF1	NP_013170.1
Schizosaccharomyces pombe (fission yeast)	SPBC1306.01c	NP_595472.2
Magnaporthe oryzae (rice blast fungus)	MGG_14797	XP_003714541.1
Arabidopsis thaliana (thale cress)	AT2G45030	NP_182029.1
Canis lupus familiaris (dog)	GFM1	XP_534320.2
Kluyveromyces lactis	KLLA0D05467g	XP_453304.1
Arabidopsis thaliana (thale cress)	AT1G45332	NP_175135.1
Mus musculus (house mouse)	Gfm1	NP_613057.2
Rattus norvegicus (Norway rat)	Gfm1	NP_446077.1
Caenorhabditis elegans (roundworm)	CELE_F29C12.4	NP_496787.1
Pan troglodytes (chimpanzee)	GFM1	XP_001154618.1
	GFM1	XP_001094832.2
Danio rerio (zebrafish)	gfm1	NP_001073463.1
Drosophila melanogaster (fruit fly)	ico	NP_609105.1
Anopheles gambiae (African malaria mosquito)	EFGM_ANOGA	XP_318822.4
Eremothecium gossypii	AGOS_ABR227C	NP_983176.2
Neurospora crassa	NCU02955	XP_965342.2
Oryza sativa (rice)	Os03g0565500	NP_001050501.1
Xenopus tropicalis (tropical clawed frog)	gfm1	XP_002933285.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT1G45332 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT1G45332 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb25719	NM_103595.4 Latest version!	Arabidopsis thaliana Translation elongation factor EFG/EF2 protein (AT1G45332), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb34224	NM_001333262.1 Latest version!	Arabidopsis thaliana Translation elongation factor EFG/EF2 protein (AT1G45332), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT1G45332 ( NM_103595.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_175135.1 Arabidopsis thaliana Translation elongation factor EFG/EF2 protein (AT1G45332), mRNA.

Price & Availability↑

CloneID	OAb25719
Clone ID Related Accession (Same CDS sequence)	NM_103595.4
Accession Version	NM_103595.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Translation elongation factor EFG/EF2 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_103595.3.

ATGGCGAGGT TTCCGACTTC TCCGGCGCCT AACCGCCTCT TGAGGCTCTT CTCTTCCAAC 
AAACGGTCGT CATCTCCTAC TGCCGCGCTT TTAACCGGAG ATTTTCAGCT GATTCGTCAT 
TTCTCCGCCG GAACCGCTGC TCGTGTTGCC AAGGATGAGA AGGAACCATG GTGGAAAGAA 
TCCATGGACA AGCTTCGCAA CATCGGGATC TCAGCTCATA TAGACTCTGG AAAGACTACG 
TTGACTGAAC GTGTTCTCTT CTATACCGGT CGGATCCATG AGATCCACGA AGTCAGAGGT 
AGAGATGGCG TCGGCGCTAA AATGGATTCC ATGGACTTAG AACGAGAGAA AGGTATCACC 
ATCCAATCAG CCGCTACCTA CTGTACTTGG AAGGATTACA AGGTTAATAT AATTGATACT 
CCTGGTCATG TTGACTTCAC CATTGAAGTG GAGAGGGCTT TACGTGTACT AGACGGTGCG 
ATTCTAGTCC TGTGCAGTGT TGGTGGTGTA CAGAGTCAGT CTATTACGGT TGATAGGCAA 
ATGAGGAGAT ATGAAGTCCC GAGAGTTGCG TTTATTAACA AGCTTGATAG GATGGGAGCA 
GATCCATGGA AAGTCTTGAA CCAAGCAAGA GCTAAGCTTC GACATCATAG TGCAGCTGTG 
CAAGTGCCCA TTGGTCTAGA AGAAAATTTT CAGGGTCTTA TAGACCTTAT TCATGTCAAA 
GCTTATTTCT TTCATGGATC AAGCGGTGAG AATGTTGTGG CTGGTGATAT TCCTGCTGAT 
ATGGAAGGTT TGGTTGCGGA GAAGAGGCGA GAATTGATAG AGACAGTCTC TGAAGTTGAT 
GATGTACTCG CCGAGAAATT TCTGAATGAT GAACCTGTAT CTGCTTCTGA GCTTGAGGAA 
GCTATTCGTA GAGCTACCAT AGCACAAACG TTTGTTCCTG TATTTATGGG TAGTGCATTC 
AAAAACAAGG GAGTACAACC TCTACTAGAT GGTGTTGTCA GTTTTCTCCC TTCTCCAAAT 
GAAGTCAATA ACTATGCTCT TGACCAGAAT AATAATGAAG AGAGGGTCAC CTTGACTGGA 
TCTCCAGATG GACCTCTTGT GGCGCTGGCT TTCAAATTAG AGGAGGGTCG TTTTGGTCAA 
CTGACATATT TGAGAGTTTA CGAAGGTGTG ATTAAGAAAG GTGATTTTAT CATAAATGTC 
AACACTGGGA AGAGAATTAA GGTTCCACGC TTGGTCCGTA TGCATTCAAA TGATATGGAG 
GATATTCAAG AGGCACATGC TGGACAAATC GTAGCTGTGT TTGGTATTGA GTGTGCATCA 
GGTGATACAT TTACGGATGG ATCAGTCAAG TACACGATGA CATCGATGAA CGTTCCTGAA 
CCTGTTATGT CCTTAGCTGT TCAACCGGTT TCAAAAGATT CTGGAGGACA GTTCTCGAAA 
GCACTGAATC GATTCCAGAA AGAGGATCCT ACTTTCCGTG TGGGTTTGGA TCCCGAGAGT 
GGCCAGACGA TTATTTCTGG TATGGGGGAA TTGCATTTGG ACATCTATGT TGAACGCATG 
CGGAGAGAAT ATAAGGTTGA TGCGACTGTG GGCAAACCCC GTGTCAATTT TAGGGAGACT 
ATTACCCAGC GCGCTGAGTT TGATTATTTA CATAAGAAGC AAAGTGGAGG AGCTGGTCAA 
TACGGTAGAG TTACAGGGTA CGTGGAACCG CTACCACCAG GCTCTAAAGA GAAATTTGAG 
TTTGAAAACA TGATTGTTGG ACAAGCAATT CCGTCGGGTT TTATTCCAGC AATTGAGAAA 
GGTTTCAAAG AAGCTGCAAA CTCGGGCTCA CTAATTGGTC ACCCTGTAGA AAATCTCAGA 
ATAGTATTGA CAGATGGAGC TTCACACGCT GTAGATTCCA GTGAACTTGC GTTTAAAATG 
GCTGCAATAT ACGCATTCAG ACTGTGTTAC ACAGCAGCTA GACCGGTGAT TCTAGAACCT 
GTTATGCTGG TTGAGTTGAA AGTACCAACA GAGTTTCAGG GCACTGTTGC TGGTGACATC 
AACAAGAGGA AAGGTATAAT CGTTGGAAAC GATCAAGAGG GTGACGATTC AGTAATCACT 
GCTAATGTGC CTTTGAACAA CATGTTTGGC TACTCCACTT CTCTCCGGTC CATGACACAA 
GGCAAAGGAG AATTCACGAT GGAATACAAA GAACATTCTG CTGTGTCCAA TGAAGTCCAG 
GCTCAGCTCG TCAATGCTTA CAGCGCCAGC AAAGCTACTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_175135.1
CDS	378..2642
Translation	MARFPTSPAPNRLLRLFSSNKRSSSPTAALLTGDFQLIRHFSAGTAARVAKDEKEPWWKESMDKLRNIGISAHIDSGKTTLTERVLFYTGRIHEIHEVRGRDGVGAKMDSMDLEREKGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRYEVPRVAFINKLDRMGADPWKVLNQARAKLRHHSAAVQVPIGLEENFQGLIDLIHVKAYFFHGSSGENVVAGDIPADMEGLVAEKRRELIETVSEVDDVLAEKFLNDEPVSASELEEAIRRATIAQTFVPVFMGSAFKNKGVQPLLDGVVSFLPSPNEVNNYALDQNNNEERVTLTGSPDGPLVALAFKLEEGRFGQLTYLRVYEGVIKKGDFIINVNTGKRIKVPRLVRMHSNDMEDIQEAHAGQIVAVFGIECASGDTFTDGSVKYTMTSMNVPEPVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYKVDATVGKPRVNFRETITQRAEFDYLHKKQSGGAGQYGRVTGYVEPLPPGSKEKFEFENMIVGQAIPSGFIPAIEKGFKEAANSGSLIGHPVENLRIVLTDGASHAVDSSELAFKMAAIYAFRLCYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDSVITANVPLNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQLVNAYSASKATE

Target ORF information:

RefSeq Version	NM_103595.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Translation elongation factor EFG/EF2 protein (AT1G45332), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_103595.4

ORF Insert Sequence:

ATGGCGAGGT TTCCGACTTC TCCGGCGCCT AACCGCCTCT TGAGGCTCTT CTCTTCCAAC 
AAACGGTCGT CATCTCCTAC TGCCGCGCTT TTAACCGGAG ATTTTCAGCT GATTCGTCAT 
TTCTCCGCCG GAACCGCTGC TCGTGTTGCC AAGGATGAGA AGGAACCATG GTGGAAAGAA 
TCCATGGACA AGCTTCGCAA CATCGGGATC TCAGCTCATA TAGACTCTGG AAAGACTACG 
TTGACTGAAC GTGTTCTCTT CTATACCGGT CGGATCCATG AGATCCACGA AGTCAGAGGT 
AGAGATGGCG TCGGCGCTAA AATGGATTCC ATGGACTTAG AACGAGAGAA AGGTATCACC 
ATCCAATCAG CCGCTACCTA CTGTACTTGG AAGGATTACA AGGTTAATAT AATTGATACT 
CCTGGTCATG TTGACTTCAC CATTGAAGTG GAGAGGGCTT TACGTGTACT AGACGGTGCG 
ATTCTAGTCC TGTGCAGTGT TGGTGGTGTA CAGAGTCAGT CTATTACGGT TGATAGGCAA 
ATGAGGAGAT ATGAAGTCCC GAGAGTTGCG TTTATTAACA AGCTTGATAG GATGGGAGCA 
GATCCATGGA AAGTCTTGAA CCAAGCAAGA GCTAAGCTTC GACATCATAG TGCAGCTGTG 
CAAGTGCCCA TTGGTCTAGA AGAAAATTTT CAGGGTCTTA TAGACCTTAT TCATGTCAAA 
GCTTATTTCT TTCATGGATC AAGCGGTGAG AATGTTGTGG CTGGTGATAT TCCTGCTGAT 
ATGGAAGGTT TGGTTGCGGA GAAGAGGCGA GAATTGATAG AGACAGTCTC TGAAGTTGAT 
GATGTACTCG CCGAGAAATT TCTGAATGAT GAACCTGTAT CTGCTTCTGA GCTTGAGGAA 
GCTATTCGTA GAGCTACCAT AGCACAAACG TTTGTTCCTG TATTTATGGG TAGTGCATTC 
AAAAACAAGG GAGTACAACC TCTACTAGAT GGTGTTGTCA GTTTTCTCCC TTCTCCAAAT 
GAAGTCAATA ACTATGCTCT TGACCAGAAT AATAATGAAG AGAGGGTCAC CTTGACTGGA 
TCTCCAGATG GACCTCTTGT GGCGCTGGCT TTCAAATTAG AGGAGGGTCG TTTTGGTCAA 
CTGACATATT TGAGAGTTTA CGAAGGTGTG ATTAAGAAAG GTGATTTTAT CATAAATGTC 
AACACTGGGA AGAGAATTAA GGTTCCACGC TTGGTCCGTA TGCATTCAAA TGATATGGAG 
GATATTCAAG AGGCACATGC TGGACAAATC GTAGCTGTGT TTGGTATTGA GTGTGCATCA 
GGTGATACAT TTACGGATGG ATCAGTCAAG TACACGATGA CATCGATGAA CGTTCCTGAA 
CCTGTTATGT CCTTAGCTGT TCAACCGGTT TCAAAAGATT CTGGAGGACA GTTCTCGAAA 
GCACTGAATC GATTCCAGAA AGAGGATCCT ACTTTCCGTG TGGGTTTGGA TCCCGAGAGT 
GGCCAGACGA TTATTTCTGG TATGGGGGAA TTGCATTTGG ACATCTATGT TGAACGCATG 
CGGAGAGAAT ATAAGGTTGA TGCGACTGTG GGCAAACCCC GTGTCAATTT TAGGGAGACT 
ATTACCCAGC GCGCTGAGTT TGATTATTTA CATAAGAAGC AAAGTGGAGG AGCTGGTCAA 
TACGGTAGAG TTACAGGGTA CGTGGAACCG CTACCACCAG GCTCTAAAGA GAAATTTGAG 
TTTGAAAACA TGATTGTTGG ACAAGCAATT CCGTCGGGTT TTATTCCAGC AATTGAGAAA 
GGTTTCAAAG AAGCTGCAAA CTCGGGCTCA CTAATTGGTC ACCCTGTAGA AAATCTCAGA 
ATAGTATTGA CAGATGGAGC TTCACACGCT GTAGATTCCA GTGAACTTGC GTTTAAAATG 
GCTGCAATAT ACGCATTCAG ACTGTGTTAC ACAGCAGCTA GACCGGTGAT TCTAGAACCT 
GTTATGCTGG TTGAGTTGAA AGTACCAACA GAGTTTCAGG GCACTGTTGC TGGTGACATC 
AACAAGAGGA AAGGTATAAT CGTTGGAAAC GATCAAGAGG GTGACGATTC AGTAATCACT 
GCTAATGTGC CTTTGAACAA CATGTTTGGC TACTCCACTT CTCTCCGGTC CATGACACAA 
GGCAAAGGAG AATTCACGAT GGAATACAAA GAACATTCTG CTGTGTCCAA TGAAGTCCAG 
GCTCAGCTCG TCAATGCTTA CAGCGCCAGC AAAGCTACTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT1G45332 ( NM_001333262.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001321287.1 Arabidopsis thaliana Translation elongation factor EFG/EF2 protein (AT1G45332), mRNA.

Price & Availability↑

CloneID	OAb34224
Clone ID Related Accession (Same CDS sequence)	NM_001333262.1
Accession Version	NM_001333262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Translation elongation factor EFG/EF2 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGAGGAGAT ATGAAGTCCC GAGAGTTGCG TTTATTAACA AGCTTGATAG GATGGGAGCA 
GATCCATGGA AAGTCTTGAA CCAAGCAAGA GCTAAGCTTC GACATCATAG TGCAGCTGTG 
CAAGTGCCCA TTGGTCTAGA AGAAAATTTT CAGGGTCTTA TAGACCTTAT TCATGTCAAA 
GCTTATTTCT TTCATGGATC AAGCGGTGAG AATGTTGTGG CTGGTGATAT TCCTGCTGAT 
ATGGAAGGTT TGGTTGCGGA GAAGAGGCGA GAATTGATAG AGACAGTCTC TGAAGTTGAT 
GATGTACTCG CCGAGAAATT TCTGAATGAT GAACCTGTAT CTGCTTCTGA GCTTGAGGAA 
GCTATTCGTA GAGCTACCAT AGCACAAACG TTTGTTCCTG TATTTATGGG TAGTGCATTC 
AAAAACAAGG GAGTACAACC TCTACTAGAT GGTGTTGTCA GTTTTCTCCC TTCTCCAAAT 
GAAGTCAATA ACTATGCTCT TGACCAGAAT AATAATGAAG AGAGGGTCAC CTTGACTGGA 
TCTCCAGATG GACCTCTTGT GGCGCTGGCT TTCAAATTAG AGGAGGGTCG TTTTGGTCAA 
CTGACATATT TGAGAGTTTA CGAAGGTGTG ATTAAGAAAG GTGATTTTAT CATAAATGTC 
AACACTGGGA AGAGAATTAA GGTTCCACGC TTGGTCCGTA TGCATTCAAA TGATATGGAG 
GATATTCAAG AGGCACATGC TGGACAAATC GTAGCTGTGT TTGGTATTGA GTGTGCATCA 
GGTGATACAT TTACGGATGG ATCAGTCAAG TACACGATGA CATCGATGAA CGTTCCTGAA 
CCTGTTATGT CCTTAGCTGT TCAACCGGTT TCAAAAGATT CTGGAGGACA GTTCTCGAAA 
GCACTGAATC GATTCCAGAA AGAGGATCCT ACTTTCCGTG TGGGTTTGGA TCCCGAGAGT 
GGCCAGACGA TTATTTCTGG TATGGGGGAA TTGCATTTGG ACATCTATGT TGAACGCATG 
CGGAGAGAAT ATAAGGTTGA TGCGACTGTG GGCAAACCCC GTGTCAATTT TAGGGAGACT 
ATTACCCAGC GCGCTGAGTT TGATTATTTA CATAAGAAGC AAAGTGGAGG AGCTGGTCAA 
TACGGTAGAG TTACAGGGTA CGTGGAACCG CTACCACCAG GCTCTAAAGA GAAATTTGAG 
TTTGAAAACA TGATTGTTGG ACAAGCAATT CCGTCGGGTT TTATTCCAGC AATTGAGAAA 
GGTTTCAAAG AAGCTGCAAA CTCGGGCTCA CTAATTGGTC ACCCTGTAGA AAATCTCAGA 
ATAGTATTGA CAGATGGAGC TTCACACGCT GTAGATTCCA GTGAACTTGC GTTTAAAATG 
GCTGCAATAT ACGCATTCAG ACTGTGTTAC ACAGCAGCTA GACCGGTGAT TCTAGAACCT 
GTTATGCTGG TTGAGTTGAA AGTACCAACA GAGTTTCAGG GCACTGTTGC TGGTGACATC 
AACAAGAGGA AAGGTATAAT CGTTGGAAAC GATCAAGAGG GTGACGATTC AGTAATCACT 
GCTAATGTGC CTTTGAACAA CATGTTTGGC TACTCCACTT CTCTCCGGTC CATGACACAA 
GGCAAAGGAG AATTCACGAT GGAATACAAA GAACATTCTG CTGTGTCCAA TGAAGTCCAG 
GCTCAGCTCG TCAATGCTTA CAGCGCCAGC AAAGCTACTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001321287.1
CDS	691..2415
Translation	MRRYEVPRVAFINKLDRMGADPWKVLNQARAKLRHHSAAVQVPIGLEENFQGLIDLIHVKAYFFHGSSGENVVAGDIPADMEGLVAEKRRELIETVSEVDDVLAEKFLNDEPVSASELEEAIRRATIAQTFVPVFMGSAFKNKGVQPLLDGVVSFLPSPNEVNNYALDQNNNEERVTLTGSPDGPLVALAFKLEEGRFGQLTYLRVYEGVIKKGDFIINVNTGKRIKVPRLVRMHSNDMEDIQEAHAGQIVAVFGIECASGDTFTDGSVKYTMTSMNVPEPVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYKVDATVGKPRVNFRETITQRAEFDYLHKKQSGGAGQYGRVTGYVEPLPPGSKEKFEFENMIVGQAIPSGFIPAIEKGFKEAANSGSLIGHPVENLRIVLTDGASHAVDSSELAFKMAAIYAFRLCYTAARPVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDSVITANVPLNNMFGYSTSLRSMTQGKGEFTMEYKEHSAVSNEVQAQLVNAYSASKATE

Target ORF information:

RefSeq Version	NM_001333262.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Translation elongation factor EFG/EF2 protein (AT1G45332), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001333262.1

ORF Insert Sequence:

ATGAGGAGAT ATGAAGTCCC GAGAGTTGCG TTTATTAACA AGCTTGATAG GATGGGAGCA 
GATCCATGGA AAGTCTTGAA CCAAGCAAGA GCTAAGCTTC GACATCATAG TGCAGCTGTG 
CAAGTGCCCA TTGGTCTAGA AGAAAATTTT CAGGGTCTTA TAGACCTTAT TCATGTCAAA 
GCTTATTTCT TTCATGGATC AAGCGGTGAG AATGTTGTGG CTGGTGATAT TCCTGCTGAT 
ATGGAAGGTT TGGTTGCGGA GAAGAGGCGA GAATTGATAG AGACAGTCTC TGAAGTTGAT 
GATGTACTCG CCGAGAAATT TCTGAATGAT GAACCTGTAT CTGCTTCTGA GCTTGAGGAA 
GCTATTCGTA GAGCTACCAT AGCACAAACG TTTGTTCCTG TATTTATGGG TAGTGCATTC 
AAAAACAAGG GAGTACAACC TCTACTAGAT GGTGTTGTCA GTTTTCTCCC TTCTCCAAAT 
GAAGTCAATA ACTATGCTCT TGACCAGAAT AATAATGAAG AGAGGGTCAC CTTGACTGGA 
TCTCCAGATG GACCTCTTGT GGCGCTGGCT TTCAAATTAG AGGAGGGTCG TTTTGGTCAA 
CTGACATATT TGAGAGTTTA CGAAGGTGTG ATTAAGAAAG GTGATTTTAT CATAAATGTC 
AACACTGGGA AGAGAATTAA GGTTCCACGC TTGGTCCGTA TGCATTCAAA TGATATGGAG 
GATATTCAAG AGGCACATGC TGGACAAATC GTAGCTGTGT TTGGTATTGA GTGTGCATCA 
GGTGATACAT TTACGGATGG ATCAGTCAAG TACACGATGA CATCGATGAA CGTTCCTGAA 
CCTGTTATGT CCTTAGCTGT TCAACCGGTT TCAAAAGATT CTGGAGGACA GTTCTCGAAA 
GCACTGAATC GATTCCAGAA AGAGGATCCT ACTTTCCGTG TGGGTTTGGA TCCCGAGAGT 
GGCCAGACGA TTATTTCTGG TATGGGGGAA TTGCATTTGG ACATCTATGT TGAACGCATG 
CGGAGAGAAT ATAAGGTTGA TGCGACTGTG GGCAAACCCC GTGTCAATTT TAGGGAGACT 
ATTACCCAGC GCGCTGAGTT TGATTATTTA CATAAGAAGC AAAGTGGAGG AGCTGGTCAA 
TACGGTAGAG TTACAGGGTA CGTGGAACCG CTACCACCAG GCTCTAAAGA GAAATTTGAG 
TTTGAAAACA TGATTGTTGG ACAAGCAATT CCGTCGGGTT TTATTCCAGC AATTGAGAAA 
GGTTTCAAAG AAGCTGCAAA CTCGGGCTCA CTAATTGGTC ACCCTGTAGA AAATCTCAGA 
ATAGTATTGA CAGATGGAGC TTCACACGCT GTAGATTCCA GTGAACTTGC GTTTAAAATG 
GCTGCAATAT ACGCATTCAG ACTGTGTTAC ACAGCAGCTA GACCGGTGAT TCTAGAACCT 
GTTATGCTGG TTGAGTTGAA AGTACCAACA GAGTTTCAGG GCACTGTTGC TGGTGACATC 
AACAAGAGGA AAGGTATAAT CGTTGGAAAC GATCAAGAGG GTGACGATTC AGTAATCACT 
GCTAATGTGC CTTTGAACAA CATGTTTGGC TACTCCACTT CTCTCCGGTC CATGACACAA 
GGCAAAGGAG AATTCACGAT GGAATACAAA GAACATTCTG CTGTGTCCAA TGAAGTCCAG 
GCTCAGCTCG TCAATGCTTA CAGCGCCAGC AAAGCTACTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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AT1G45332 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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