ESP3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESP3
Entrez Gene ID	840143
Full Name	RNA helicase family protein
Synonyms	EMB2733,EMBRYO DEFECTIVE 2733,ENHANCED SILENCING PHENOTYPE 3,F5D14.27,F5D14_27
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_102984.5	NP_174527.2	RNA helicase family protein
NM_001198198.1	NP_001185127.1	RNA helicase family protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	DHX16	NP_003578.2
Rattus norvegicus (Norway rat)	Dhx16	NP_997661.1
Caenorhabditis elegans (roundworm)	mog-4	NP_497027.1
Schizosaccharomyces pombe (fission yeast)	cdc28	NP_595686.2
Neurospora crassa	NCU08928	XP_960795.1
Drosophila melanogaster (fruit fly)	l(2)37Cb	NP_609946.1
Pan troglodytes (chimpanzee)	DHX16	NP_001035839.1
	DHX16	NP_001098636.1
Mus musculus (house mouse)	Dhx16	NP_081263.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009093	XP_319843.4
Arabidopsis thaliana (thale cress)	ESP3	NP_174527.2
Canis lupus familiaris (dog)	DHX16	XP_538827.2
Danio rerio (zebrafish)	dhx16	NP_956318.1
Bos taurus (cattle)	DHX16	NP_001193139.1
Magnaporthe oryzae (rice blast fungus)	MGG_07501	XP_003711413.1
Arabidopsis thaliana (thale cress)	MEE29	NP_181077.3
Oryza sativa (rice)	Os05g0389800	NP_001055437.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data showed that ESP3 is a helicase involved in mRNA splicing.
Title: A temperature-sensitive allele of a putative mRNA splicing helicase down-regulates many cell wall genes and causes radial swelling in Arabidopsis thaliana.

The following ESP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb25341	NM_102984.5 Latest version!	Arabidopsis thaliana RNA helicase family protein (ESP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb29848	NM_001198198.1 Latest version!	Arabidopsis thaliana RNA helicase family protein (ESP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESP3 ( NM_102984.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_174527.2 Arabidopsis thaliana RNA helicase family protein (ESP3), mRNA.

Price & Availability↑

CloneID	OAb25341
Clone ID Related Accession (Same CDS sequence)	NM_102984.5
Accession Version	NM_102984.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA helicase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_102984.4.

ATGGCGAGCA ATGATTTGAA GACTTGGGTT TCGGATAAGC TGATGATGCT ACTTGGCTAC 
TCTCAAGCGG CTGTTGTTAA CTATCTCATT GCAATGGCTA AAAAAACAAA ATCACCAACT 
GAACTTGTGG GAGAGTTGGT GGATTACGGG TTTTCGTCTT CTGGTGATAC TCGTTCGTTT 
GCAGAAGAAA TTTTTGCCAG AGTTCCCCGT AAAACTGCTG GTGTAAATCT CTACCAAAAA 
CATGAAGCAG AGGCAGCAAT GCTAGTGAGG AAGCAGAAAA CATATGCTCT TTTAGATGCT 
GATGATGATG AAGACGAAGT TGTTGTGGAG AAAAAATCTT CAGTTTCTGA ATCTAGAAAA 
TCAGATAAGG GCAAGAAAAG GTTTAGAAAG AAAAGTGGGC AGTCAGATGA AAGTGATGGC 
GAGGTGGCCG TTAGAGAGGA TAGTAGACAC GTTAGAAGAA AGGTTTCTGA AGAGGATGAT 
GGTTCTGAGT CAGAAGAGGA AAGAGTGCGT GACCAAAAGG AGAGAGAAGA ACTGGAGCAG 
CATTTAAAAG ATCGTGATAC TGCACGGACA CGAAAGCTAA CAGAGCAAAC GTTGTCAAAG 
AAAGAGAAAG AAGAGGCTGT TCGAAGAGCA AATGCTTTGG AGAAGGATGA TTTATATTCC 
TTAAGGAAAG TCTCGAGACA AGAATACTTA AAGAAGAGAG AACAAAAGAA ACTGGATGAA 
CTAAGGGATG AGATAGAAGA TGAACAATAT CTTTTCGGAG GTGAAAAGCT CACAGAAACG 
GAGCTTCGTG AGTTTAGGTA CAAGAAAGAG CTTTATGACC TCGTGAAGAA AAGAACACAA 
GATGAAGATA ACGTCGAGGA GTATAGAATT CCAGATGCTT ATGATCAAGA GGGAGGTGTT 
GATCAGGAGA AGAGGTTTTC TGTTGCTGTT CAACGATACA GGGACCTGGA TTCAACAGAG 
AAAATGAATC CATTCGCAGA GCAAGAAGCT TGGGAGGACC ATCAGATTGG AAAAGCAACA 
TTAAAATTTG GTGCAAAGAA TAAGCAAGCC TCAGACGATT ATCAGTTTGT GTTTGAAGAC 
CAAATAAACT TCATAAAGGA ATCTGTCATG GCTGGTGAAA ATTATGAAGA TGCTATGGAT 
GCAAAACAAA AATCTCAAGA TTTGGCAGAG AAAACAGCTT TAGAGGAACT TCAGGAAGTC 
AGAAGAAGTC TGCCGATTTA TACGTATCGT GATCAACTGC TTAAGGCTGT GGAAGAGCAT 
CAGGTTCTTG TCATTGTGGG AGACACTGGT TCAGGAAAAA CGACACAAAT ACCACAATAT 
CTCCATGAAG CTGGTTACAC AAAGCGTGGA AAGGTTGGTT GTACGCAGCC CCGAAGAGTT 
GCAGCTATGA GTGTTGCTGC CCGTGTGGCT CAAGAGATGG GTGTCAAACT CGGACATGAG 
GTTGGCTATT CCATTCGATT TGAGGATTGT ACTTCAGACA AAACAGTTCT GAAGTATATG 
ACTGATGGAA TGCTTTTGCG CGAGCTGCTT GGTGAGCCAG ACCTGGCCAG CTACAGTGTC 
GTTATTGTGG ATGAGGCACA TGAAAGGACC TTGTCAACCG ATATACTGTT TGGATTAGTC 
AAGGATATAG CGCGGTTTCG ACCTGATTTG AAGTTGTTGA TATCTAGTGC AACAATGGAT 
GCAGAAAAAT TTTCTGATTA TTTTGATACA GCCCCGATTT TTAGTTTTCC TGGAAGAAGG 
TATCCAGTTG AAATCAACTA TACAAGTGCA CCTGAAGCTG ATTACATGGA CGCAGCCATA 
GTTACTATCC TTACGATCCA TGTGAGGGAG CCACTTGGAG ATATATTGGT CTTCTTCACT 
GGTCAAGAGG AAATTGAAAC TGCAGAAGAG ATCTTGAAGC ACAGGATAAG AGGTTTGGGT 
ACAAAGATCC GTGAATTAAT CATATGCCCA ATTTACGCAA ATCTTCCAAG TGAACTCCAA 
GCAAAAATAT TTGAGCCAAC TCCAGAAGGG GCACGTAAAG TTGTCCTAGC AACAAATATC 
GCCGAAACAT CATTGACAAT TGATGGTATA AAGTATGTTG TTGATCCAGG CTTTAGCAAG 
ATGAAGTCAT ATAACCCGAG GACAGGGATG GAATCTTTGC TTATTACTCC TATCTCTAAG 
GCTTCTGCAA CTCAACGAGC TGGTCGTGCT GGAAGAACAA GTCCAGGGAA GTGTTATCGT 
CTGTACACTG CGTTTAATTA CAACAATGAC TTGGAGGAAA ACACGGTGCC AGAAGTACAG 
AGGACGAATC TTGCTAGTGT GGTGCTTGCT TTGAAGAGTC TTGGAATTCA TGATCTCATA 
AACTTCGATT TCATGGACCC TCCACCTGCT GAAGCACTTG TAAAGTCTTT AGAACTGCTC 
TTTGCTCTGG GTGCTCTAAA TAAGCTCGGG GAATTGACTA AAGCTGGTAG AAGGATGGCA 
GAGTTCCCCC TTGATCCAAT GCTGTCGAAA ATGATTGTTG TTTCTGACAA GTATAAGTGC 
TCCGATGAGA TAATATCAAT CGCTGCTATG TTGTCAATTG GTGGCTCTAT CTTCTACCGT 
CCCAAGGACA AACAAGTTCA TGCGGACAAC GCAAGAATGA ATTTCCACAC CGGGAATGTC 
GGTGACCATA TTGCTTTGCT AAAGGTTTAC AGTTCATGGA AGGAAACAAA CTTCTCTACT 
CAGTGGTGCT ACGAGAACTA CATTCAGGTC CGGAGCATGA AAAGAGCCAG AGATATCCGT 
GATCAATTGG AGGGACTTCT CGAAAGGGTG GAAATAGATA TCAGCTCAAA CCTGAATGAA 
TTGGATTCAG TTAGAAAATC CATCGTAGCC GGGTTTTTTC CGCACACTGC AAAGCTACAG 
AAGAATGGAT CGTACCGAAC AGTGAAACAT CCCCAGACAG TGCACATACA TCCCAATTCG 
GGCTTATCTC AGGTATTGCC GAGATGGGTT GTATATCACG AACTAGTACT TACGTCCAAG 
GAATACATGC GACAGGTAAC AGAGTTGAAA CCAGAGTGGT TGATTGAGTT AGCTCCTCAC 
TACTACCAGC TCAAGGACGT TGAAGACGCT GCATCAAAGA AAATGCCCAA AGGAGCTGGA 
AAAGCTGCAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_174527.2
CDS	224..3358
Translation	MASNDLKTWVSDKLMMLLGYSQAAVVNYLIAMAKKTKSPTELVGELVDYGFSSSGDTRSFAEEIFARVPRKTAGVNLYQKHEAEAAMLVRKQKTYALLDADDDEDEVVVEKKSSVSESRKSDKGKKRFRKKSGQSDESDGEVAVREDSRHVRRKVSEEDDGSESEEERVRDQKEREELEQHLKDRDTARTRKLTEQTLSKKEKEEAVRRANALEKDDLYSLRKVSRQEYLKKREQKKLDELRDEIEDEQYLFGGEKLTETELREFRYKKELYDLVKKRTQDEDNVEEYRIPDAYDQEGGVDQEKRFSVAVQRYRDLDSTEKMNPFAEQEAWEDHQIGKATLKFGAKNKQASDDYQFVFEDQINFIKESVMAGENYEDAMDAKQKSQDLAEKTALEELQEVRRSLPIYTYRDQLLKAVEEHQVLVIVGDTGSGKTTQIPQYLHEAGYTKRGKVGCTQPRRVAAMSVAARVAQEMGVKLGHEVGYSIRFEDCTSDKTVLKYMTDGMLLRELLGEPDLASYSVVIVDEAHERTLSTDILFGLVKDIARFRPDLKLLISSATMDAEKFSDYFDTAPIFSFPGRRYPVEINYTSAPEADYMDAAIVTILTIHVREPLGDILVFFTGQEEIETAEEILKHRIRGLGTKIRELIICPIYANLPSELQAKIFEPTPEGARKVVLATNIAETSLTIDGIKYVVDPGFSKMKSYNPRTGMESLLITPISKASATQRAGRAGRTSPGKCYRLYTAFNYNNDLEENTVPEVQRTNLASVVLALKSLGIHDLINFDFMDPPPAEALVKSLELLFALGALNKLGELTKAGRRMAEFPLDPMLSKMIVVSDKYKCSDEIISIAAMLSIGGSIFYRPKDKQVHADNARMNFHTGNVGDHIALLKVYSSWKETNFSTQWCYENYIQVRSMKRARDIRDQLEGLLERVEIDISSNLNELDSVRKSIVAGFFPHTAKLQKNGSYRTVKHPQTVHIHPNSGLSQVLPRWVVYHELVLTSKEYMRQVTELKPEWLIELAPHYYQLKDVEDAASKKMPKGAGKAAM

Target ORF information:

RefSeq Version	NM_102984.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA helicase family protein (ESP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_102984.5

ORF Insert Sequence:

ATGGCGAGCA ATGATTTGAA GACTTGGGTT TCGGATAAGC TGATGATGCT ACTTGGCTAC 
TCTCAAGCGG CTGTTGTTAA CTATCTCATT GCAATGGCTA AAAAAACAAA ATCACCAACT 
GAACTTGTGG GAGAGTTGGT GGATTACGGG TTTTCGTCTT CTGGTGATAC TCGTTCGTTT 
GCAGAAGAAA TTTTTGCCAG AGTTCCCCGT AAAACTGCTG GTGTAAATCT CTACCAAAAA 
CATGAAGCAG AGGCAGCAAT GCTAGTGAGG AAGCAGAAAA CATATGCTCT TTTAGATGCT 
GATGATGATG AAGACGAAGT TGTTGTGGAG AAAAAATCTT CAGTTTCTGA ATCTAGAAAA 
TCAGATAAGG GCAAGAAAAG GTTTAGAAAG AAAAGTGGGC AGTCAGATGA AAGTGATGGC 
GAGGTGGCCG TTAGAGAGGA TAGTAGACAC GTTAGAAGAA AGGTTTCTGA AGAGGATGAT 
GGTTCTGAGT CAGAAGAGGA AAGAGTGCGT GACCAAAAGG AGAGAGAAGA ACTGGAGCAG 
CATTTAAAAG ATCGTGATAC TGCACGGACA CGAAAGCTAA CAGAGCAAAC GTTGTCAAAG 
AAAGAGAAAG AAGAGGCTGT TCGAAGAGCA AATGCTTTGG AGAAGGATGA TTTATATTCC 
TTAAGGAAAG TCTCGAGACA AGAATACTTA AAGAAGAGAG AACAAAAGAA ACTGGATGAA 
CTAAGGGATG AGATAGAAGA TGAACAATAT CTTTTCGGAG GTGAAAAGCT CACAGAAACG 
GAGCTTCGTG AGTTTAGGTA CAAGAAAGAG CTTTATGACC TCGTGAAGAA AAGAACACAA 
GATGAAGATA ACGTCGAGGA GTATAGAATT CCAGATGCTT ATGATCAAGA GGGAGGTGTT 
GATCAGGAGA AGAGGTTTTC TGTTGCTGTT CAACGATACA GGGACCTGGA TTCAACAGAG 
AAAATGAATC CATTCGCAGA GCAAGAAGCT TGGGAGGACC ATCAGATTGG AAAAGCAACA 
TTAAAATTTG GTGCAAAGAA TAAGCAAGCC TCAGACGATT ATCAGTTTGT GTTTGAAGAC 
CAAATAAACT TCATAAAGGA ATCTGTCATG GCTGGTGAAA ATTATGAAGA TGCTATGGAT 
GCAAAACAAA AATCTCAAGA TTTGGCAGAG AAAACAGCTT TAGAGGAACT TCAGGAAGTC 
AGAAGAAGTC TGCCGATTTA TACGTATCGT GATCAACTGC TTAAGGCTGT GGAAGAGCAT 
CAGGTTCTTG TCATTGTGGG AGACACTGGT TCAGGAAAAA CGACACAAAT ACCACAATAT 
CTCCATGAAG CTGGTTACAC AAAGCGTGGA AAGGTTGGTT GTACGCAGCC CCGAAGAGTT 
GCAGCTATGA GTGTTGCTGC CCGTGTGGCT CAAGAGATGG GTGTCAAACT CGGACATGAG 
GTTGGCTATT CCATTCGATT TGAGGATTGT ACTTCAGACA AAACAGTTCT GAAGTATATG 
ACTGATGGAA TGCTTTTGCG CGAGCTGCTT GGTGAGCCAG ACCTGGCCAG CTACAGTGTC 
GTTATTGTGG ATGAGGCACA TGAAAGGACC TTGTCAACCG ATATACTGTT TGGATTAGTC 
AAGGATATAG CGCGGTTTCG ACCTGATTTG AAGTTGTTGA TATCTAGTGC AACAATGGAT 
GCAGAAAAAT TTTCTGATTA TTTTGATACA GCCCCGATTT TTAGTTTTCC TGGAAGAAGG 
TATCCAGTTG AAATCAACTA TACAAGTGCA CCTGAAGCTG ATTACATGGA CGCAGCCATA 
GTTACTATCC TTACGATCCA TGTGAGGGAG CCACTTGGAG ATATATTGGT CTTCTTCACT 
GGTCAAGAGG AAATTGAAAC TGCAGAAGAG ATCTTGAAGC ACAGGATAAG AGGTTTGGGT 
ACAAAGATCC GTGAATTAAT CATATGCCCA ATTTACGCAA ATCTTCCAAG TGAACTCCAA 
GCAAAAATAT TTGAGCCAAC TCCAGAAGGG GCACGTAAAG TTGTCCTAGC AACAAATATC 
GCCGAAACAT CATTGACAAT TGATGGTATA AAGTATGTTG TTGATCCAGG CTTTAGCAAG 
ATGAAGTCAT ATAACCCGAG GACAGGGATG GAATCTTTGC TTATTACTCC TATCTCTAAG 
GCTTCTGCAA CTCAACGAGC TGGTCGTGCT GGAAGAACAA GTCCAGGGAA GTGTTATCGT 
CTGTACACTG CGTTTAATTA CAACAATGAC TTGGAGGAAA ACACGGTGCC AGAAGTACAG 
AGGACGAATC TTGCTAGTGT GGTGCTTGCT TTGAAGAGTC TTGGAATTCA TGATCTCATA 
AACTTCGATT TCATGGACCC TCCACCTGCT GAAGCACTTG TAAAGTCTTT AGAACTGCTC 
TTTGCTCTGG GTGCTCTAAA TAAGCTCGGG GAATTGACTA AAGCTGGTAG AAGGATGGCA 
GAGTTCCCCC TTGATCCAAT GCTGTCGAAA ATGATTGTTG TTTCTGACAA GTATAAGTGC 
TCCGATGAGA TAATATCAAT CGCTGCTATG TTGTCAATTG GTGGCTCTAT CTTCTACCGT 
CCCAAGGACA AACAAGTTCA TGCGGACAAC GCAAGAATGA ATTTCCACAC CGGGAATGTC 
GGTGACCATA TTGCTTTGCT AAAGGTTTAC AGTTCATGGA AGGAAACAAA CTTCTCTACT 
CAGTGGTGCT ACGAGAACTA CATTCAGGTC CGGAGCATGA AAAGAGCCAG AGATATCCGT 
GATCAATTGG AGGGACTTCT CGAAAGGGTG GAAATAGATA TCAGCTCAAA CCTGAATGAA 
TTGGATTCAG TTAGAAAATC CATCGTAGCC GGGTTTTTTC CGCACACTGC AAAGCTACAG 
AAGAATGGAT CGTACCGAAC AGTGAAACAT CCCCAGACAG TGCACATACA TCCCAATTCG 
GGCTTATCTC AGGTATTGCC GAGATGGGTT GTATATCACG AACTAGTACT TACGTCCAAG 
GAATACATGC GACAGGTAAC AGAGTTGAAA CCAGAGTGGT TGATTGAGTT AGCTCCTCAC 
TACTACCAGC TCAAGGACGT TGAAGACGCT GCATCAAAGA AAATGCCCAA AGGAGCTGGA 
AAAGCTGCAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESP3 ( NM_001198198.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185127.1 Arabidopsis thaliana RNA helicase family protein (ESP3), mRNA.

Price & Availability↑

CloneID	OAb29848
Clone ID Related Accession (Same CDS sequence)	NM_001198198.1
Accession Version	NM_001198198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	RNA helicase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGCGAGCA ATGATTTGAA GACTTGGGTT TCGGATAAGC TGATGATGCT ACTTGGCTAC 
TCTCAAGCGG CTGTTGTTAA CTATCTCATT GCAATGGCTA AAAAAACAAA ATCACCAACT 
GAACTTGTGG GAGAGTTGGT GGATTACGGG TTTTCGTCTT CTGGTGATAC TCGTTCGTTT 
GCAGAAGAAA TTTTTGCCAG AGTTCCCCGT AAAACTGCTG GTGTAAATCT CTACCAAAAA 
CATGAAGCAG AGGCAGCAAT GCTAGTGAGG AAGCAGAAAA CATATGCTCT TTTAGATGCT 
GATGATGATG AAGACGAAGT TGTTGTGGAG AAAAAATCTT CAGTTTCTGA ATCTAGAAAA 
TCAGATAAGG GCAAGAAAAG GTTTAGAAAG AAAAGTGGGC AGTCAGATGA AAGTGATGGC 
GAGGTGGCCG TTAGAGAGGA TAGTAGACAC GTTAGAAGAA AGGTTTCTGA AGAGGATGAT 
GGTTCTGAGT CAGAAGAGGA AAGAGTGCGT GACCAAAAGG AGAGAGAAGA ACTGGAGCAG 
CATTTAAAAG ATCGTGATAC TGCACGGACA CGAAAGCTAA CAGAGCAAAC GTTGTCAAAG 
AAAGAGAAAG AAGAGGCTGT TCGAAGAGCA AATGCTTTGG AGAAGGATGA TTTATATTCC 
TTAAGGAAAG TCTCGAGACA AGAATACTTA AAGAAGAGAG AACAAAAGAA ACTGGATGAA 
CTAAGGGATG AGATAGAAGA TGAACAATAT CTTTTCGGAG GTGAAAAGCT CACAGAAACG 
GAGCTTCGTG AGTTTAGGTA CAAGAAAGAG CTTTATGACC TCGTGAAGAA AAGAACACAA 
GATGAAGATA ACGTCGAGGA GGGAGGTGTT GATCAGGAGA AGAGGTTTTC TGTTGCTGTT 
CAACGATACA GGGACCTGGA TTCAACAGAG AAAATGAATC CATTCGCAGA GCAAGAAGCT 
TGGGAGGACC ATCAGATTGG AAAAGCAACA TTAAAATTTG GTGCAAAGAA TAAGCAAGCC 
TCAGACGATT ATCAGTTTGT GTTTGAAGAC CAAATAAACT TCATAAAGGA ATCTGTCATG 
GCTGGTGAAA ATTATGAAGA TGCTATGGAT GCAAAACAAA AATCTCAAGA TTTGGCAGAG 
AAAACAGCTT TAGAGGAACT TCAGGAAGTC AGAAGAAGTC TGCCGATTTA TACGTATCGT 
GATCAACTGC TTAAGGCTGT GGAAGAGCAT CAGGTTCTTG TCATTGTGGG AGACACTGGT 
TCAGGAAAAA CGACACAAAT ACCACAATAT CTCCATGAAG CTGGTTACAC AAAGCGTGGA 
AAGGTTGGTT GTACGCAGCC CCGAAGAGTT GCAGCTATGA GTGTTGCTGC CCGTGTGGCT 
CAAGAGATGG GTGTCAAACT CGGACATGAG GTTGGCTATT CCATTCGATT TGAGGATTGT 
ACTTCAGACA AAACAGTTCT GAAGTATATG ACTGATGGAA TGCTTTTGCG CGAGCTGCTT 
GGTGAGCCAG ACCTGGCCAG CTACAGTGTC GTTATTGTGG ATGAGGCACA TGAAAGGACC 
TTGTCAACCG ATATACTGTT TGGATTAGTC AAGGATATAG CGCGGTTTCG ACCTGATTTG 
AAGTTGTTGA TATCTAGTGC AACAATGGAT GCAGAAAAAT TTTCTGATTA TTTTGATACA 
GCCCCGATTT TTAGTTTTCC TGGAAGAAGG TATCCAGTTG AAATCAACTA TACAAGTGCA 
CCTGAAGCTG ATTACATGGA CGCAGCCATA GTTACTATCC TTACGATCCA TGTGAGGGAG 
CCACTTGGAG ATATATTGGT CTTCTTCACT GGTCAAGAGG AAATTGAAAC TGCAGAAGAG 
ATCTTGAAGC ACAGGATAAG AGGTTTGGGT ACAAAGATCC GTGAATTAAT CATATGCCCA 
ATTTACGCAA ATCTTCCAAG TGAACTCCAA GCAAAAATAT TTGAGCCAAC TCCAGAAGGG 
GCACGTAAAG TTGTCCTAGC AACAAATATC GCCGAAACAT CATTGACAAT TGATGGTATA 
AAGTATGTTG TTGATCCAGG CTTTAGCAAG ATGAAGTCAT ATAACCCGAG GACAGGGATG 
GAATCTTTGC TTATTACTCC TATCTCTAAG GCTTCTGCAA CTCAACGAGC TGGTCGTGCT 
GGAAGAACAA GTCCAGGGAA GTGTTATCGT CTGTACACTG CGTTTAATTA CAACAATGAC 
TTGGAGGAAA ACACGGTGCC AGAAGTACAG AGGACGAATC TTGCTAGTGT GGTGCTTGCT 
TTGAAGAGTC TTGGAATTCA TGATCTCATA AACTTCGATT TCATGGACCC TCCACCTGCT 
GAAGCACTTG TAAAGTCTTT AGAACTGCTC TTTGCTCTGG GTGCTCTAAA TAAGCTCGGG 
GAATTGACTA AAGCTGGTAG AAGGATGGCA GAGTTCCCCC TTGATCCAAT GCTGTCGAAA 
ATGATTGTTG TTTCTGACAA GTATAAGTGC TCCGATGAGA TAATATCAAT CGCTGCTATG 
TTGTCAATTG GTGGCTCTAT CTTCTACCGT CCCAAGGACA AACAAGTTCA TGCGGACAAC 
GCAAGAATGA ATTTCCACAC CGGGAATGTC GGTGACCATA TTGCTTTGCT AAAGGTTTAC 
AGTTCATGGA AGGAAACAAA CTTCTCTACT CAGTGGTGCT ACGAGAACTA CATTCAGGTC 
CGGAGCATGA AAAGAGCCAG AGATATCCGT GATCAATTGG AGGGACTTCT CGAAAGGGTG 
GAAATAGATA TCAGCTCAAA CCTGAATGAA TTGGATTCAG TTAGAAAATC CATCGTAGCC 
GGGTTTTTTC CGCACACTGC AAAGCTACAG AAGAATGGAT CGTACCGAAC AGTGAAACAT 
CCCCAGACAG TGCACATACA TCCCAATTCG GGCTTATCTC AGGTATTGCC GAGATGGGTT 
GTATATCACG AACTAGTACT TACGTCCAAG GAATACATGC GACAGGTAAC AGAGTTGAAA 
CCAGAGTGGT TGATTGAGTT AGCTCCTCAC TACTACCAGC TCAAGGACGT TGAAGACGCT 
GCATCAAAGA AAATGCCCAA AGGAGCTGGA AAAGCTGCAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185127.1
CDS	229..3333
Translation	MASNDLKTWVSDKLMMLLGYSQAAVVNYLIAMAKKTKSPTELVGELVDYGFSSSGDTRSFAEEIFARVPRKTAGVNLYQKHEAEAAMLVRKQKTYALLDADDDEDEVVVEKKSSVSESRKSDKGKKRFRKKSGQSDESDGEVAVREDSRHVRRKVSEEDDGSESEEERVRDQKEREELEQHLKDRDTARTRKLTEQTLSKKEKEEAVRRANALEKDDLYSLRKVSRQEYLKKREQKKLDELRDEIEDEQYLFGGEKLTETELREFRYKKELYDLVKKRTQDEDNVEEGGVDQEKRFSVAVQRYRDLDSTEKMNPFAEQEAWEDHQIGKATLKFGAKNKQASDDYQFVFEDQINFIKESVMAGENYEDAMDAKQKSQDLAEKTALEELQEVRRSLPIYTYRDQLLKAVEEHQVLVIVGDTGSGKTTQIPQYLHEAGYTKRGKVGCTQPRRVAAMSVAARVAQEMGVKLGHEVGYSIRFEDCTSDKTVLKYMTDGMLLRELLGEPDLASYSVVIVDEAHERTLSTDILFGLVKDIARFRPDLKLLISSATMDAEKFSDYFDTAPIFSFPGRRYPVEINYTSAPEADYMDAAIVTILTIHVREPLGDILVFFTGQEEIETAEEILKHRIRGLGTKIRELIICPIYANLPSELQAKIFEPTPEGARKVVLATNIAETSLTIDGIKYVVDPGFSKMKSYNPRTGMESLLITPISKASATQRAGRAGRTSPGKCYRLYTAFNYNNDLEENTVPEVQRTNLASVVLALKSLGIHDLINFDFMDPPPAEALVKSLELLFALGALNKLGELTKAGRRMAEFPLDPMLSKMIVVSDKYKCSDEIISIAAMLSIGGSIFYRPKDKQVHADNARMNFHTGNVGDHIALLKVYSSWKETNFSTQWCYENYIQVRSMKRARDIRDQLEGLLERVEIDISSNLNELDSVRKSIVAGFFPHTAKLQKNGSYRTVKHPQTVHIHPNSGLSQVLPRWVVYHELVLTSKEYMRQVTELKPEWLIELAPHYYQLKDVEDAASKKMPKGAGKAAM

Target ORF information:

RefSeq Version	NM_001198198.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA helicase family protein (ESP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198198.1

ORF Insert Sequence:

ATGGCGAGCA ATGATTTGAA GACTTGGGTT TCGGATAAGC TGATGATGCT ACTTGGCTAC 
TCTCAAGCGG CTGTTGTTAA CTATCTCATT GCAATGGCTA AAAAAACAAA ATCACCAACT 
GAACTTGTGG GAGAGTTGGT GGATTACGGG TTTTCGTCTT CTGGTGATAC TCGTTCGTTT 
GCAGAAGAAA TTTTTGCCAG AGTTCCCCGT AAAACTGCTG GTGTAAATCT CTACCAAAAA 
CATGAAGCAG AGGCAGCAAT GCTAGTGAGG AAGCAGAAAA CATATGCTCT TTTAGATGCT 
GATGATGATG AAGACGAAGT TGTTGTGGAG AAAAAATCTT CAGTTTCTGA ATCTAGAAAA 
TCAGATAAGG GCAAGAAAAG GTTTAGAAAG AAAAGTGGGC AGTCAGATGA AAGTGATGGC 
GAGGTGGCCG TTAGAGAGGA TAGTAGACAC GTTAGAAGAA AGGTTTCTGA AGAGGATGAT 
GGTTCTGAGT CAGAAGAGGA AAGAGTGCGT GACCAAAAGG AGAGAGAAGA ACTGGAGCAG 
CATTTAAAAG ATCGTGATAC TGCACGGACA CGAAAGCTAA CAGAGCAAAC GTTGTCAAAG 
AAAGAGAAAG AAGAGGCTGT TCGAAGAGCA AATGCTTTGG AGAAGGATGA TTTATATTCC 
TTAAGGAAAG TCTCGAGACA AGAATACTTA AAGAAGAGAG AACAAAAGAA ACTGGATGAA 
CTAAGGGATG AGATAGAAGA TGAACAATAT CTTTTCGGAG GTGAAAAGCT CACAGAAACG 
GAGCTTCGTG AGTTTAGGTA CAAGAAAGAG CTTTATGACC TCGTGAAGAA AAGAACACAA 
GATGAAGATA ACGTCGAGGA GGGAGGTGTT GATCAGGAGA AGAGGTTTTC TGTTGCTGTT 
CAACGATACA GGGACCTGGA TTCAACAGAG AAAATGAATC CATTCGCAGA GCAAGAAGCT 
TGGGAGGACC ATCAGATTGG AAAAGCAACA TTAAAATTTG GTGCAAAGAA TAAGCAAGCC 
TCAGACGATT ATCAGTTTGT GTTTGAAGAC CAAATAAACT TCATAAAGGA ATCTGTCATG 
GCTGGTGAAA ATTATGAAGA TGCTATGGAT GCAAAACAAA AATCTCAAGA TTTGGCAGAG 
AAAACAGCTT TAGAGGAACT TCAGGAAGTC AGAAGAAGTC TGCCGATTTA TACGTATCGT 
GATCAACTGC TTAAGGCTGT GGAAGAGCAT CAGGTTCTTG TCATTGTGGG AGACACTGGT 
TCAGGAAAAA CGACACAAAT ACCACAATAT CTCCATGAAG CTGGTTACAC AAAGCGTGGA 
AAGGTTGGTT GTACGCAGCC CCGAAGAGTT GCAGCTATGA GTGTTGCTGC CCGTGTGGCT 
CAAGAGATGG GTGTCAAACT CGGACATGAG GTTGGCTATT CCATTCGATT TGAGGATTGT 
ACTTCAGACA AAACAGTTCT GAAGTATATG ACTGATGGAA TGCTTTTGCG CGAGCTGCTT 
GGTGAGCCAG ACCTGGCCAG CTACAGTGTC GTTATTGTGG ATGAGGCACA TGAAAGGACC 
TTGTCAACCG ATATACTGTT TGGATTAGTC AAGGATATAG CGCGGTTTCG ACCTGATTTG 
AAGTTGTTGA TATCTAGTGC AACAATGGAT GCAGAAAAAT TTTCTGATTA TTTTGATACA 
GCCCCGATTT TTAGTTTTCC TGGAAGAAGG TATCCAGTTG AAATCAACTA TACAAGTGCA 
CCTGAAGCTG ATTACATGGA CGCAGCCATA GTTACTATCC TTACGATCCA TGTGAGGGAG 
CCACTTGGAG ATATATTGGT CTTCTTCACT GGTCAAGAGG AAATTGAAAC TGCAGAAGAG 
ATCTTGAAGC ACAGGATAAG AGGTTTGGGT ACAAAGATCC GTGAATTAAT CATATGCCCA 
ATTTACGCAA ATCTTCCAAG TGAACTCCAA GCAAAAATAT TTGAGCCAAC TCCAGAAGGG 
GCACGTAAAG TTGTCCTAGC AACAAATATC GCCGAAACAT CATTGACAAT TGATGGTATA 
AAGTATGTTG TTGATCCAGG CTTTAGCAAG ATGAAGTCAT ATAACCCGAG GACAGGGATG 
GAATCTTTGC TTATTACTCC TATCTCTAAG GCTTCTGCAA CTCAACGAGC TGGTCGTGCT 
GGAAGAACAA GTCCAGGGAA GTGTTATCGT CTGTACACTG CGTTTAATTA CAACAATGAC 
TTGGAGGAAA ACACGGTGCC AGAAGTACAG AGGACGAATC TTGCTAGTGT GGTGCTTGCT 
TTGAAGAGTC TTGGAATTCA TGATCTCATA AACTTCGATT TCATGGACCC TCCACCTGCT 
GAAGCACTTG TAAAGTCTTT AGAACTGCTC TTTGCTCTGG GTGCTCTAAA TAAGCTCGGG 
GAATTGACTA AAGCTGGTAG AAGGATGGCA GAGTTCCCCC TTGATCCAAT GCTGTCGAAA 
ATGATTGTTG TTTCTGACAA GTATAAGTGC TCCGATGAGA TAATATCAAT CGCTGCTATG 
TTGTCAATTG GTGGCTCTAT CTTCTACCGT CCCAAGGACA AACAAGTTCA TGCGGACAAC 
GCAAGAATGA ATTTCCACAC CGGGAATGTC GGTGACCATA TTGCTTTGCT AAAGGTTTAC 
AGTTCATGGA AGGAAACAAA CTTCTCTACT CAGTGGTGCT ACGAGAACTA CATTCAGGTC 
CGGAGCATGA AAAGAGCCAG AGATATCCGT GATCAATTGG AGGGACTTCT CGAAAGGGTG 
GAAATAGATA TCAGCTCAAA CCTGAATGAA TTGGATTCAG TTAGAAAATC CATCGTAGCC 
GGGTTTTTTC CGCACACTGC AAAGCTACAG AAGAATGGAT CGTACCGAAC AGTGAAACAT 
CCCCAGACAG TGCACATACA TCCCAATTCG GGCTTATCTC AGGTATTGCC GAGATGGGTT 
GTATATCACG AACTAGTACT TACGTCCAAG GAATACATGC GACAGGTAAC AGAGTTGAAA 
CCAGAGTGGT TGATTGAGTT AGCTCCTCAC TACTACCAGC TCAAGGACGT TGAAGACGCT 
GCATCAAAGA AAATGCCCAA AGGAGCTGGA AAAGCTGCAA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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ESP3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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