DCL1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCL1
Entrez Gene ID	839574
Full Name	dicer-like 1
Synonyms	ABNORMAL SUSPENSOR 1,ASU1,ATDCL1,CAF,CARPEL FACTORY,DICER-LIKE 1,EMB60,EMB76,EMBRYO DEFECTIVE 60,EMBRYO DEFECTIVE 76,SHORT INTEGUMENTS 1,SIN1,SUS1,SUSPENSOR 1,T25K16.4,T25K16_4,dicer-like 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_099986.4	NP_171612.1	dicer-like 1
NM_001197952.1	NP_001184881.1	dicer-like 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Further analysis revealed that the steady state level of HYL1 protein in plants under osmotic stress is dependent on the SnRK2 kinases. Additionally, our results suggest that the SnRK2 kinases physically associate with the miRNA processing components SE and HYL1 and can phosphorylate these proteins in vitro.
Title: The SnRK2 kinases modulate miRNA accumulation in Arabidopsis.

Lariat RNAs localized in nuclear bodies, and partially co-localize with HYL1, and both DCL1 and HYL1 were mis-localized in dbr1-2... we thus propose that lariat RNAs, as decoys, inhibit miRNA processing, suggesting a hitherto unknown layer of regulation in miRNA biogenesis.
Title: Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis.

a detailed modeling and molecular dynamics study of dsRNA recognition by DCL1-1.
Title: dsRNA-protein interactions studied by molecular dynamics techniques. Unravelling dsRNA recognition by DCL1.

CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs.
Title: The Roles of Arabidopsis CDF2 in Transcriptional and Posttranscriptional Regulation of Primary MicroRNAs.

The first double stranded RNA binding domain (dsRBD) of DCL1 is intrinsically disordered, but it can still perform its expected role, binding dsRNA and working jointly with the second dsRBD in the recognition of the substrate.
Title: Induced folding in RNA recognition by Arabidopsis thaliana DCL1.

The following DCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb24902	NM_099986.4 Latest version!	Arabidopsis thaliana dicer-like 1 (DCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb30026	NM_001197952.1 Latest version!	Arabidopsis thaliana dicer-like 1 (DCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCL1 ( NM_099986.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_171612.1 Arabidopsis thaliana dicer-like 1 (DCL1), mRNA.

Price & Availability↑

CloneID	OAb24902
Clone ID Related Accession (Same CDS sequence)	NM_099986.4
Accession Version	NM_099986.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_099986.3.

ATGGTAATGG AGGATGAGCC TAGAGAAGCC ACAATAAAGC CTTCTTATTG GCTAGATGCT 
TGCGAGGACA TCTCTTGTGA TCTTATCGAT GATCTCGTGT CTGAATTTGA TCCTTCCTCT 
GTTGCTGTCA ATGAATCCAC TGATGAAAAC GGCGTCATCA ATGATTTTTT CGGTGGGATT 
GATCACATTT TAGATAGTAT CAAGAACGGT GGAGGCTTAC CAAACAATGG CGTTTCTGAT 
ACCAATTCTC AAATCAACGA GGTTACTGTA ACTCCTCAGG TTATTGCTAA GGAGACAGTG 
AAGGAGAATG GGTTGCAAAA GAATGGCGGT AAGAGAGACG AATTCTCGAA AGAGGAAGGA 
GACAAGGATA GGAAGAGAGC TAGGGTTTGT AGTTATCAGA GTGAAAGGAG TAACCTTTCA 
GGTAGAGGGC ATGTTAATAA TTCTAGGGAG GGAGATAGGT TTATGAATAG GAAACGTACT 
CGTAATTGGG ACGAGGCGGG TAACAATAAG AAGAAAAGGG AATGTAACAA TTACAGAAGA 
GATGGTAGAG ATAGAGAAGT TAGGGGTTAT TGGGAGAGGG ATAAAGTTGG TTCCAATGAG 
TTGGTTTATA GGTCAGGGAC TTGGGAAGCT GATCATGAAA GAGATGTTAA GAAAGTGAGT 
GGTGGAAACC GCGAATGCGA TGTCAAGGCA GAGGAGAACA AGAGTAAGCC TGAAGAACGT 
AAAGAGAAGG TTGTGGAAGA GCAAGCAAGG CGATACCAGT TGGATGTTCT TGAACAAGCT 
AAAGCGAAAA ACACGATTGC TTTCCTTGAG ACCGGTGCTG GAAAGACACT TATCGCGATT 
CTTCTTATTA AAAGTGTTCA TAAGGATCTG ATGAGCCAGA ACAGAAAAAT GCTCTCGGTG 
TTCTTGGTTC CCAAAGTGCC TTTGGTTTAT CAGCAAGCAG AAGTGATCCG TAATCAAACT 
TGTTTTCAAG TTGGACATTA TTGTGGTGAG ATGGGACAGG ACTTTTGGGA TTCTCGAAGG 
TGGCAACGAG AGTTTGAGTC TAAGCAGGTT CTAGTTATGA CAGCACAAAT TCTGTTGAAT 
ATACTGAGAC ACAGTATCAT TAGAATGGAA ACAATTGATC TTCTTATTCT CGACGAGTGT 
CACCACGCTG TCAAGAAACA TCCATACTCT TTAGTGATGT CAGAGTTTTA CCATACAACT 
CCTAAAGATA AAAGACCTGC CATCTTTGGA ATGACTGCTT CGCCTGTTAA TTTAAAGGGT 
GTTTCAAGCC AAGTAGATTG TGCGATAAAG ATACGTAACC TCGAGACCAA GTTGGATTCT 
ACGGTTTGTA CTATAAAAGA TCGAAAAGAA TTAGAGAAAC ATGTGCCTAT GCCTTCAGAG 
ATAGTCGTCG AGTATGACAA AGCTGCTACT ATGTGGTCTC TTCATGAGAC AATAAAGCAA 
ATGATTGCAG CTGTTGAAGA AGCGGCACAA GCAAGTTCAA GGAAAAGCAA GTGGCAATTT 
ATGGGGGCTA GGGATGCTGG AGCAAAGGAT GAATTGAGAC AGGTTTATGG CGTCTCTGAA 
AGAACGGAGA GCGATGGTGC TGCCAATTTG ATTCATAAAC TTAGAGCTAT CAATTATACT 
CTTGCTGAAT TGGGTCAATG GTGTGCTTAC AAGGTGGGAC AATCATTCTT GTCTGCTTTG 
CAAAGTGATG AGAGGGTGAA TTTCCAAGTC GACGTGAAGT TTCAAGAATC ATACCTCAGT 
GAGGTGGTGT CACTCTTGCA ATGTGAGCTT CTGGAAGGCG CTGCTGCTGA AAAAGTCGCG 
GCGGAAGTTG GCAAACCAGA AAATGGTAAT GCACATGACG AGATGGAGGA GGGAGAGCTC 
CCTGATGATC CTGTGGTCTC GGGAGGGGAG CACGTTGATG AAGTAATAGG CGCCGCAGTG 
GCTGATGGGA AAGTTACTCC AAAAGTACAA TCATTGATCA AACTACTCCT CAAATATCAG 
CACACAGCTG ATTTTCGAGC TATTGTTTTC GTTGAGAGGG TGGTTGCTGC TTTGGTTCTT 
CCTAAGGTTT TTGCGGAGCT GCCTTCGCTT AGTTTTATAC GGTGTGCCAG CATGATTGGA 
CACAATAACA GCCAGGAGAT GAAATCATCT CAAATGCAGG ATACAATTTC CAAATTCCGA 
GATGGGCATG TGACACTGTT AGTTGCCACA AGCGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGTAACG TTGTTATGCG TTTCGACCTT GCAAAGACGG TGCTGGCATA CATTCAGTCT 
CGTGGCCGGG CAAGAAAGCC TGGATCAGAC TACATACTCA TGGTTGAGAG AGGAAATGTA 
TCTCACGCAG CGTTCCTAAG GAATGCTAGG AACAGTGAGG AGACACTTCG AAAAGAAGCA 
ATAGAAAGGA CTGATCTTAG TCATCTCAAA GATACATCGA GATTAATCTC AATTGATGCT 
GTGCCTGGTA CAGTTTATAA GGTGGAGGCA ACTGGTGCCA TGGTTAGCTT GAATTCCGCG 
GTTGGTCTTG TACATTTCTA CTGCTCTCAG CTTCCTGGTG ACAGGTATGC AATCCTTCGT 
CCTGAGTTTA GCATGGAGAA GCATGAAAAG CCTGGGGGCC ACACGGAATA TTCATGTAGG 
CTTCAGCTTC CTTGCAATGC ACCGTTTGAA ATACTTGAGG GTCCTGTTTG CAGTTCAATG 
CGTCTTGCAC AACAGGCTGT ATGTTTAGCT GCTTGCAAGA AACTGCATGA GATGGGTGCA 
TTTACCGATA TGCTATTACC GGACAAAGGA AGTGGTCAAG ACGCTGAGAA GGCTGACCAA 
GATGATGAAG GTGAGCCTGT TCCTGGAACT GCTAGACATA GAGAGTTCTA TCCTGAAGGT 
GTGGCGGATG TACTTAAGGG AGAATGGGTT TCATCTGGAA AGGAAGTTTG TGAGAGCTCA 
AAGCTATTCC ATTTATACAT GTATAATGTC AGATGTGTAG ATTTTGGCTC TTCAAAAGAT 
CCATTCCTAA GCGAAGTTTC AGAGTTCGCG ATTCTTTTTG GCAATGAGCT GGATGCAGAG 
GTATTATCGA TGTCTATGGA TCTTTATGTT GCTCGGGCCA TGATCACTAA AGCATCTCTT 
GCTTTCAAGG GATCACTTGA TATTACAGAA AACCAGCTAT CATCTCTAAA AAAGTTTCAT 
GTGAGGTTAA TGAGTATCGT GTTGGATGTT GATGTTGAAC CCTCCACGAC ACCATGGGAT 
CCTGCAAAGG CCTACCTGTT TGTCCCTGTT ACTGACAATA CGTCTATGGA ACCCATAAAA 
GGGATCAACT GGGAATTGGT TGAAAAGATT ACGAAAACCA CAGCGTGGGA CAACCCTCTT 
CAGAGAGCTC GTCCCGATGT ATATCTCGGG ACTAATGAGA GAACTCTTGG TGGGGACAGA 
AGGGAATATG GGTTTGGTAA ACTTCGTCAC AACATTGTAT TTGGGCAGAA ATCTCACCCA 
ACTTATGGTA TTAGAGGAGC TGTTGCATCC TTCGATGTTG TGAGAGCTTC TGGATTGTTA 
CCTGTGAGAG ATGCTTTTGA GAAGGAAGTA GAAGAGGATT TATCAAAAGG AAAATTGATG 
ATGGCTGATG GGTGCATGGT TGCAGAAGAT CTTATTGGGA AAATAGTGAC AGCCGCACAT 
TCCGGGAAGC GGTTTTACGT AGATTCAATT TGTTATGACA TGAGTGCAGA AACATCTTTC 
CCTAGGAAAG AGGGATATCT TGGTCCCCTA GAGTACAACA CGTACGCTGA CTATTACAAG 
CAAAAGTATG GAGTTGATTT GAACTGTAAG CAACAACCTT TGATTAAAGG ACGTGGTGTT 
TCGTATTGCA AGAACCTTCT TTCTCCTCGG TTTGAACAGT CAGGTGAATC TGAGACAGTC 
CTTGATAAGA CATATTACGT GTTTCTTCCA CCTGAACTAT GCGTTGTGCA TCCGCTTTCG 
GGTTCACTTA TCCGAGGTGC TCAGAGGTTA CCCTCTATAA TGAGAAGAGT TGAGAGCATG 
TTACTCGCTG TTCAACTCAA AAATTTGATT AGTTATCCTA TTCCCACATC AAAGATTCTT 
GAAGCCTTGA CTGCCGCCTC GTGCCAGGAA ACGTTCTGCT ACGAGAGAGC TGAGCTTTTA 
GGAGATGCGT ATCTAAAATG GGTTGTTAGT CGTTTTCTGT TTCTCAAGTA TCCTCAAAAG 
CACGAGGGTC AGCTTACAAG GATGAGGCAA CAAATGGTTA GTAATATGGT TCTTTATCAG 
TTTGCTCTGG TTAAAGGGCT TCAGTCATAT ATCCAGGCGG ATCGATTCGC CCCGTCTAGG 
TGGTCTGCTC CTGGTGTGCC TCCGGTTTTC GACGAGGACA CAAAAGATGG AGGATCTTCG 
TTTTTCGATG AAGAGCAAAA ACCTGTTTCC GAGGAAAACA GCGATGTGTT TGAAGATGGG 
GAGATGGAGG ATGGTGAACT AGAGGGTGAT TTGAGTTCGT ACCGAGTTTT ATCTAGCAAA 
ACGTTAGCTG ATGTTGTTGA GGCTTTGATT GGTGTTTATT ACGTCGAAGG GGGTAAGATT 
GCAGCTAATC ATTTGATGAA ATGGATTGGG ATTCACGTGG AGGATGATCC TGATGAAGTC 
GATGGAACAT TGAAAAATGT TAATGTTCCA GAGAGTGTGC TCAAGAGCAT CGACTTTGTT 
GGTCTTGAGA GAGCTCTTAA ATATGAGTTT AAAGAGAAAG GTCTTCTTGT TGAAGCTATA 
ACACATGCTT CAAGACCATC TTCAGGTGTT TCGTGTTACC AGAGATTGGA ATTTGTTGGT 
GACGCGGTCT TGGATCATCT CATCACAAGA CATCTATTTT TCACATACAC AAGCCTTCCT 
CCTGGTCGGT TAACAGATCT TCGAGCTGCA GCGGTTAACA ACGAGAATTT TGCTCGCGTT 
GCGGTTAAAC ATAAACTCCA CTTGTACCTT CGTCACGGTT CAAGCGCCCT CGAAAAACAG 
ATTCGGGAAT TTGTGAAGGA GGTTCAAACC GAGTCATCGA AACCGGGGTT TAACTCTTTT 
GGTTTGGGAG ACTGCAAAGC ACCAAAAGTT CTTGGAGACA TTGTTGAATC TATTGCAGGT 
GCTATTTTTC TTGATAGTGG AAAAGATACA ACTGCTGCTT GGAAGGTTTT TCAACCTTTG 
CTTCAGCCCA TGGTGACACC AGAGACACTT CCAATGCATC CGGTGCGAGA GCTACAAGAG 
CGGTGCCAGC AACAAGCAGA AGGGTTAGAA TACAAAGCGA GTAGGAGTGG TAACACAGCG 
ACTGTGGAAG TTTTCATCGA CGGTGTTCAA GTTGGAGTAG CGCAAAACCC GCAGAAGAAA 
ATGGCTCAAA AGCTAGCTGC GAGGAACGCA CTTGCAGCTT TGAAAGAGAA AGAAATAGCA 
GAATCAAAGG AGAAGCATAT CAACAACGGT AATGCGGGAG AGGATCAAGG CGAGAATGAG 
AATGGGAACA AGAAGAATGG GCATCAGCCG TTTACGAGAC AAACGTTGAA TGATATTTGT 
TTGAGGAAGA ATTGGCCAAT GCCTTCTTAC AGATGTGTGA AAGAAGGAGG ACCGGCTCAT 
GCAAAGAGAT TTACGTTTGG GGTAAGAGTT AATACGAGCG ATAGAGGATG GACCGATGAG 
TGTATTGGCG AGCCAATGCC GAGTGTTAAG AAAGCTAAGG ATTCAGCTGC GGTTCTTCTA 
CTTGAGCTTT TAAATAAAAC TTTTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_171612.1
CDS	399..6128
Translation	MVMEDEPREATIKPSYWLDACEDISCDLIDDLVSEFDPSSVAVNESTDENGVINDFFGGIDHILDSIKNGGGLPNNGVSDTNSQINEVTVTPQVIAKETVKENGLQKNGGKRDEFSKEEGDKDRKRARVCSYQSERSNLSGRGHVNNSREGDRFMNRKRTRNWDEAGNNKKKRECNNYRRDGRDREVRGYWERDKVGSNELVYRSGTWEADHERDVKKVSGGNRECDVKAEENKSKPEERKEKVVEEQARRYQLDVLEQAKAKNTIAFLETGAGKTLIAILLIKSVHKDLMSQNRKMLSVFLVPKVPLVYQQAEVIRNQTCFQVGHYCGEMGQDFWDSRRWQREFESKQVLVMTAQILLNILRHSIIRMETIDLLILDECHHAVKKHPYSLVMSEFYHTTPKDKRPAIFGMTASPVNLKGVSSQVDCAIKIRNLETKLDSTVCTIKDRKELEKHVPMPSEIVVEYDKAATMWSLHETIKQMIAAVEEAAQASSRKSKWQFMGARDAGAKDELRQVYGVSERTESDGAANLIHKLRAINYTLAELGQWCAYKVGQSFLSALQSDERVNFQVDVKFQESYLSEVVSLLQCELLEGAAAEKVAAEVGKPENGNAHDEMEEGELPDDPVVSGGEHVDEVIGAAVADGKVTPKVQSLIKLLLKYQHTADFRAIVFVERVVAALVLPKVFAELPSLSFIRCASMIGHNNSQEMKSSQMQDTISKFRDGHVTLLVATSVAEEGLDIRQCNVVMRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNVSHAAFLRNARNSEETLRKEAIERTDLSHLKDTSRLISIDAVPGTVYKVEATGAMVSLNSAVGLVHFYCSQLPGDRYAILRPEFSMEKHEKPGGHTEYSCRLQLPCNAPFEILEGPVCSSMRLAQQAVCLAACKKLHEMGAFTDMLLPDKGSGQDAEKADQDDEGEPVPGTARHREFYPEGVADVLKGEWVSSGKEVCESSKLFHLYMYNVRCVDFGSSKDPFLSEVSEFAILFGNELDAEVLSMSMDLYVARAMITKASLAFKGSLDITENQLSSLKKFHVRLMSIVLDVDVEPSTTPWDPAKAYLFVPVTDNTSMEPIKGINWELVEKITKTTAWDNPLQRARPDVYLGTNERTLGGDRREYGFGKLRHNIVFGQKSHPTYGIRGAVASFDVVRASGLLPVRDAFEKEVEEDLSKGKLMMADGCMVAEDLIGKIVTAAHSGKRFYVDSICYDMSAETSFPRKEGYLGPLEYNTYADYYKQKYGVDLNCKQQPLIKGRGVSYCKNLLSPRFEQSGESETVLDKTYYVFLPPELCVVHPLSGSLIRGAQRLPSIMRRVESMLLAVQLKNLISYPIPTSKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVSNMVLYQFALVKGLQSYIQADRFAPSRWSAPGVPPVFDEDTKDGGSSFFDEEQKPVSEENSDVFEDGEMEDGELEGDLSSYRVLSSKTLADVVEALIGVYYVEGGKIAANHLMKWIGIHVEDDPDEVDGTLKNVNVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEKEIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGGPAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNKTFS

Target ORF information:

RefSeq Version	NM_099986.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 1 (DCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_099986.4

ORF Insert Sequence:

ATGGTAATGG AGGATGAGCC TAGAGAAGCC ACAATAAAGC CTTCTTATTG GCTAGATGCT 
TGCGAGGACA TCTCTTGTGA TCTTATCGAT GATCTCGTGT CTGAATTTGA TCCTTCCTCT 
GTTGCTGTCA ATGAATCCAC TGATGAAAAC GGCGTCATCA ATGATTTTTT CGGTGGGATT 
GATCACATTT TAGATAGTAT CAAGAACGGT GGAGGCTTAC CAAACAATGG CGTTTCTGAT 
ACCAATTCTC AAATCAACGA GGTTACTGTA ACTCCTCAGG TTATTGCTAA GGAGACAGTG 
AAGGAGAATG GGTTGCAAAA GAATGGCGGT AAGAGAGACG AATTCTCGAA AGAGGAAGGA 
GACAAGGATA GGAAGAGAGC TAGGGTTTGT AGTTATCAGA GTGAAAGGAG TAACCTTTCA 
GGTAGAGGGC ATGTTAATAA TTCTAGGGAG GGAGATAGGT TTATGAATAG GAAACGTACT 
CGTAATTGGG ACGAGGCGGG TAACAATAAG AAGAAAAGGG AATGTAACAA TTACAGAAGA 
GATGGTAGAG ATAGAGAAGT TAGGGGTTAT TGGGAGAGGG ATAAAGTTGG TTCCAATGAG 
TTGGTTTATA GGTCAGGGAC TTGGGAAGCT GATCATGAAA GAGATGTTAA GAAAGTGAGT 
GGTGGAAACC GCGAATGCGA TGTCAAGGCA GAGGAGAACA AGAGTAAGCC TGAAGAACGT 
AAAGAGAAGG TTGTGGAAGA GCAAGCAAGG CGATACCAGT TGGATGTTCT TGAACAAGCT 
AAAGCGAAAA ACACGATTGC TTTCCTTGAG ACCGGTGCTG GAAAGACACT TATCGCGATT 
CTTCTTATTA AAAGTGTTCA TAAGGATCTG ATGAGCCAGA ACAGAAAAAT GCTCTCGGTG 
TTCTTGGTTC CCAAAGTGCC TTTGGTTTAT CAGCAAGCAG AAGTGATCCG TAATCAAACT 
TGTTTTCAAG TTGGACATTA TTGTGGTGAG ATGGGACAGG ACTTTTGGGA TTCTCGAAGG 
TGGCAACGAG AGTTTGAGTC TAAGCAGGTT CTAGTTATGA CAGCACAAAT TCTGTTGAAT 
ATACTGAGAC ACAGTATCAT TAGAATGGAA ACAATTGATC TTCTTATTCT CGACGAGTGT 
CACCACGCTG TCAAGAAACA TCCATACTCT TTAGTGATGT CAGAGTTTTA CCATACAACT 
CCTAAAGATA AAAGACCTGC CATCTTTGGA ATGACTGCTT CGCCTGTTAA TTTAAAGGGT 
GTTTCAAGCC AAGTAGATTG TGCGATAAAG ATACGTAACC TCGAGACCAA GTTGGATTCT 
ACGGTTTGTA CTATAAAAGA TCGAAAAGAA TTAGAGAAAC ATGTGCCTAT GCCTTCAGAG 
ATAGTCGTCG AGTATGACAA AGCTGCTACT ATGTGGTCTC TTCATGAGAC AATAAAGCAA 
ATGATTGCAG CTGTTGAAGA AGCGGCACAA GCAAGTTCAA GGAAAAGCAA GTGGCAATTT 
ATGGGGGCTA GGGATGCTGG AGCAAAGGAT GAATTGAGAC AGGTTTATGG CGTCTCTGAA 
AGAACGGAGA GCGATGGTGC TGCCAATTTG ATTCATAAAC TTAGAGCTAT CAATTATACT 
CTTGCTGAAT TGGGTCAATG GTGTGCTTAC AAGGTGGGAC AATCATTCTT GTCTGCTTTG 
CAAAGTGATG AGAGGGTGAA TTTCCAAGTC GACGTGAAGT TTCAAGAATC ATACCTCAGT 
GAGGTGGTGT CACTCTTGCA ATGTGAGCTT CTGGAAGGCG CTGCTGCTGA AAAAGTCGCG 
GCGGAAGTTG GCAAACCAGA AAATGGTAAT GCACATGACG AGATGGAGGA GGGAGAGCTC 
CCTGATGATC CTGTGGTCTC GGGAGGGGAG CACGTTGATG AAGTAATAGG CGCCGCAGTG 
GCTGATGGGA AAGTTACTCC AAAAGTACAA TCATTGATCA AACTACTCCT CAAATATCAG 
CACACAGCTG ATTTTCGAGC TATTGTTTTC GTTGAGAGGG TGGTTGCTGC TTTGGTTCTT 
CCTAAGGTTT TTGCGGAGCT GCCTTCGCTT AGTTTTATAC GGTGTGCCAG CATGATTGGA 
CACAATAACA GCCAGGAGAT GAAATCATCT CAAATGCAGG ATACAATTTC CAAATTCCGA 
GATGGGCATG TGACACTGTT AGTTGCCACA AGCGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGTAACG TTGTTATGCG TTTCGACCTT GCAAAGACGG TGCTGGCATA CATTCAGTCT 
CGTGGCCGGG CAAGAAAGCC TGGATCAGAC TACATACTCA TGGTTGAGAG AGGAAATGTA 
TCTCACGCAG CGTTCCTAAG GAATGCTAGG AACAGTGAGG AGACACTTCG AAAAGAAGCA 
ATAGAAAGGA CTGATCTTAG TCATCTCAAA GATACATCGA GATTAATCTC AATTGATGCT 
GTGCCTGGTA CAGTTTATAA GGTGGAGGCA ACTGGTGCCA TGGTTAGCTT GAATTCCGCG 
GTTGGTCTTG TACATTTCTA CTGCTCTCAG CTTCCTGGTG ACAGGTATGC AATCCTTCGT 
CCTGAGTTTA GCATGGAGAA GCATGAAAAG CCTGGGGGCC ACACGGAATA TTCATGTAGG 
CTTCAGCTTC CTTGCAATGC ACCGTTTGAA ATACTTGAGG GTCCTGTTTG CAGTTCAATG 
CGTCTTGCAC AACAGGCTGT ATGTTTAGCT GCTTGCAAGA AACTGCATGA GATGGGTGCA 
TTTACCGATA TGCTATTACC GGACAAAGGA AGTGGTCAAG ACGCTGAGAA GGCTGACCAA 
GATGATGAAG GTGAGCCTGT TCCTGGAACT GCTAGACATA GAGAGTTCTA TCCTGAAGGT 
GTGGCGGATG TACTTAAGGG AGAATGGGTT TCATCTGGAA AGGAAGTTTG TGAGAGCTCA 
AAGCTATTCC ATTTATACAT GTATAATGTC AGATGTGTAG ATTTTGGCTC TTCAAAAGAT 
CCATTCCTAA GCGAAGTTTC AGAGTTCGCG ATTCTTTTTG GCAATGAGCT GGATGCAGAG 
GTATTATCGA TGTCTATGGA TCTTTATGTT GCTCGGGCCA TGATCACTAA AGCATCTCTT 
GCTTTCAAGG GATCACTTGA TATTACAGAA AACCAGCTAT CATCTCTAAA AAAGTTTCAT 
GTGAGGTTAA TGAGTATCGT GTTGGATGTT GATGTTGAAC CCTCCACGAC ACCATGGGAT 
CCTGCAAAGG CCTACCTGTT TGTCCCTGTT ACTGACAATA CGTCTATGGA ACCCATAAAA 
GGGATCAACT GGGAATTGGT TGAAAAGATT ACGAAAACCA CAGCGTGGGA CAACCCTCTT 
CAGAGAGCTC GTCCCGATGT ATATCTCGGG ACTAATGAGA GAACTCTTGG TGGGGACAGA 
AGGGAATATG GGTTTGGTAA ACTTCGTCAC AACATTGTAT TTGGGCAGAA ATCTCACCCA 
ACTTATGGTA TTAGAGGAGC TGTTGCATCC TTCGATGTTG TGAGAGCTTC TGGATTGTTA 
CCTGTGAGAG ATGCTTTTGA GAAGGAAGTA GAAGAGGATT TATCAAAAGG AAAATTGATG 
ATGGCTGATG GGTGCATGGT TGCAGAAGAT CTTATTGGGA AAATAGTGAC AGCCGCACAT 
TCCGGGAAGC GGTTTTACGT AGATTCAATT TGTTATGACA TGAGTGCAGA AACATCTTTC 
CCTAGGAAAG AGGGATATCT TGGTCCCCTA GAGTACAACA CGTACGCTGA CTATTACAAG 
CAAAAGTATG GAGTTGATTT GAACTGTAAG CAACAACCTT TGATTAAAGG ACGTGGTGTT 
TCGTATTGCA AGAACCTTCT TTCTCCTCGG TTTGAACAGT CAGGTGAATC TGAGACAGTC 
CTTGATAAGA CATATTACGT GTTTCTTCCA CCTGAACTAT GCGTTGTGCA TCCGCTTTCG 
GGTTCACTTA TCCGAGGTGC TCAGAGGTTA CCCTCTATAA TGAGAAGAGT TGAGAGCATG 
TTACTCGCTG TTCAACTCAA AAATTTGATT AGTTATCCTA TTCCCACATC AAAGATTCTT 
GAAGCCTTGA CTGCCGCCTC GTGCCAGGAA ACGTTCTGCT ACGAGAGAGC TGAGCTTTTA 
GGAGATGCGT ATCTAAAATG GGTTGTTAGT CGTTTTCTGT TTCTCAAGTA TCCTCAAAAG 
CACGAGGGTC AGCTTACAAG GATGAGGCAA CAAATGGTTA GTAATATGGT TCTTTATCAG 
TTTGCTCTGG TTAAAGGGCT TCAGTCATAT ATCCAGGCGG ATCGATTCGC CCCGTCTAGG 
TGGTCTGCTC CTGGTGTGCC TCCGGTTTTC GACGAGGACA CAAAAGATGG AGGATCTTCG 
TTTTTCGATG AAGAGCAAAA ACCTGTTTCC GAGGAAAACA GCGATGTGTT TGAAGATGGG 
GAGATGGAGG ATGGTGAACT AGAGGGTGAT TTGAGTTCGT ACCGAGTTTT ATCTAGCAAA 
ACGTTAGCTG ATGTTGTTGA GGCTTTGATT GGTGTTTATT ACGTCGAAGG GGGTAAGATT 
GCAGCTAATC ATTTGATGAA ATGGATTGGG ATTCACGTGG AGGATGATCC TGATGAAGTC 
GATGGAACAT TGAAAAATGT TAATGTTCCA GAGAGTGTGC TCAAGAGCAT CGACTTTGTT 
GGTCTTGAGA GAGCTCTTAA ATATGAGTTT AAAGAGAAAG GTCTTCTTGT TGAAGCTATA 
ACACATGCTT CAAGACCATC TTCAGGTGTT TCGTGTTACC AGAGATTGGA ATTTGTTGGT 
GACGCGGTCT TGGATCATCT CATCACAAGA CATCTATTTT TCACATACAC AAGCCTTCCT 
CCTGGTCGGT TAACAGATCT TCGAGCTGCA GCGGTTAACA ACGAGAATTT TGCTCGCGTT 
GCGGTTAAAC ATAAACTCCA CTTGTACCTT CGTCACGGTT CAAGCGCCCT CGAAAAACAG 
ATTCGGGAAT TTGTGAAGGA GGTTCAAACC GAGTCATCGA AACCGGGGTT TAACTCTTTT 
GGTTTGGGAG ACTGCAAAGC ACCAAAAGTT CTTGGAGACA TTGTTGAATC TATTGCAGGT 
GCTATTTTTC TTGATAGTGG AAAAGATACA ACTGCTGCTT GGAAGGTTTT TCAACCTTTG 
CTTCAGCCCA TGGTGACACC AGAGACACTT CCAATGCATC CGGTGCGAGA GCTACAAGAG 
CGGTGCCAGC AACAAGCAGA AGGGTTAGAA TACAAAGCGA GTAGGAGTGG TAACACAGCG 
ACTGTGGAAG TTTTCATCGA CGGTGTTCAA GTTGGAGTAG CGCAAAACCC GCAGAAGAAA 
ATGGCTCAAA AGCTAGCTGC GAGGAACGCA CTTGCAGCTT TGAAAGAGAA AGAAATAGCA 
GAATCAAAGG AGAAGCATAT CAACAACGGT AATGCGGGAG AGGATCAAGG CGAGAATGAG 
AATGGGAACA AGAAGAATGG GCATCAGCCG TTTACGAGAC AAACGTTGAA TGATATTTGT 
TTGAGGAAGA ATTGGCCAAT GCCTTCTTAC AGATGTGTGA AAGAAGGAGG ACCGGCTCAT 
GCAAAGAGAT TTACGTTTGG GGTAAGAGTT AATACGAGCG ATAGAGGATG GACCGATGAG 
TGTATTGGCG AGCCAATGCC GAGTGTTAAG AAAGCTAAGG ATTCAGCTGC GGTTCTTCTA 
CTTGAGCTTT TAAATAAAAC TTTTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCL1 ( NM_001197952.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001184881.1 Arabidopsis thaliana dicer-like 1 (DCL1), mRNA.

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CloneID	OAb30026
Clone ID Related Accession (Same CDS sequence)	NM_001197952.1
Accession Version	NM_001197952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	dicer-like 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGTAATGG AGGATGAGCC TAGAGAAGCC ACAATAAAGC CTTCTTATTG GCTAGATGCT 
TGCGAGGACA TCTCTTGTGA TCTTATCGAT GATCTCGTGT CTGAATTTGA TCCTTCCTCT 
GTTGCTGTCA ATGAATCCAC TGATGAAAAC GGCGTCATCA ATGATTTTTT CGGTGGGATT 
GATCACATTT TAGATAGTAT CAAGAACGGT GGAGGCTTAC CAAACAATGG CGTTTCTGAT 
ACCAATTCTC AAATCAACGA GGTTACTGTA ACTCCTCAGG TTATTGCTAA GGAGACAGTG 
AAGGAGAATG GGTTGCAAAA GAATGGCGGT AAGAGAGACG AATTCTCGAA AGAGGAAGGA 
GACAAGGATA GGAAGAGAGC TAGGGTTTGT AGTTATCAGA GTGAAAGGAG TAACCTTTCA 
GGTAGAGGGC ATGTTAATAA TTCTAGGGAG GGAGATAGGT TTATGAATAG GAAACGTACT 
CGTAATTGGG ACGAGGCGGG TAACAATAAG AAGAAAAGGG AATGTAACAA TTACAGAAGA 
GATGGTAGAG ATAGAGAAGT TAGGGGTTAT TGGGAGAGGG ATAAAGTTGG TTCCAATGAG 
TTGGTTTATA GGTCAGGGAC TTGGGAAGCT GATCATGAAA GAGATGTTAA GAAAGTGAGT 
GGTGGAAACC GCGAATGCGA TGTCAAGGCA GAGGAGAACA AGAGTAAGCC TGAAGAACGT 
AAAGAGAAGG TTGTGGAAGA GCAAGCAAGG CGATACCAGT TGGATGTTCT TGAACAAGCT 
AAAGCGAAAA ACACGATTGC TTTCCTTGAG ACCGGTGCTG GAAAGACACT TATCGCGATT 
CTTCTTATTA AAAGTGTTCA TAAGGATCTG ATGAGCCAGA ACAGAAAAAT GCTCTCGGTG 
TTCTTGGTTC CCAAAGTGCC TTTGGTTTAT CAGCAAGCAG AAGTGATCCG TAATCAAACT 
TGTTTTCAAG TTGGACATTA TTGTGGTGAG ATGGGACAGG ACTTTTGGGA TTCTCGAAGG 
TGGCAACGAG AGTTTGAGTC TAAGCAGGTT CTAGTTATGA CAGCACAAAT TCTGTTGAAT 
ATACTGAGAC ACAGTATCAT TAGAATGGAA ACAATTGATC TTCTTATTCT CGACGAGTGT 
CACCACGCTG TCAAGAAACA TCCATACTCT TTAGTGATGT CAGAGTTTTA CCATACAACT 
CCTAAAGATA AAAGACCTGC CATCTTTGGA ATGACTGCTT CGCCTGTTAA TTTAAAGGGT 
GTTTCAAGCC AAGTAGATTG TGCGATAAAG ATACGTAACC TCGAGACCAA GTTGGATTCT 
ACGGTTTGTA CTATAAAAGA TCGAAAAGAA TTAGAGAAAC ATGTGCCTAT GCCTTCAGAG 
ATAGTCGTCG AGTATGACAA AGCTGCTACT ATGTGGTCTC TTCATGAGAC AATAAAGCAA 
ATGATTGCAG CTGTTGAAGA AGCGGCACAA GCAAGTTCAA GGAAAAGCAA GTGGCAATTT 
ATGGGGGCTA GGGATGCTGG AGCAAAGGAT GAATTGAGAC AGGTTTATGG CGTCTCTGAA 
AGAACGGAGA GCGATGGTGC TGCCAATTTG ATTCATAAAC TTAGAGCTAT CAATTATACT 
CTTGCTGAAT TGGGTCAATG GTGTGCTTAC AAGGTGGGAC AATCATTCTT GTCTGCTTTG 
CAAAGTGATG AGAGGGTGAA TTTCCAAGTC GACGTGAAGT TTCAAGAATC ATACCTCAGT 
GAGGTGGTGT CACTCTTGCA ATGTGAGCTT CTGGAAGGCG CTGCTGCTGA AAAAGTCGCG 
GCGGAAGTTG GCAAACCAGA AAATGGTAAT GCACATGACG AGATGGAGGA GGGAGAGCTC 
CCTGATGATC CTGTGGTCTC GGGAGGGGAG CACGTTGATG AAGTAATAGG CGCCGCAGTG 
GCTGATGGGA AAGTTACTCC AAAAGTACAA TCATTGATCA AACTACTCCT CAAATATCAG 
CACACAGCTG ATTTTCGAGC TATTGTTTTC GTTGAGAGGG TGGTTGCTGC TTTGGTTCTT 
CCTAAGGTTT TTGCGGAGCT GCCTTCGCTT AGTTTTATAC GGTGTGCCAG CATGATTGGA 
CACAATAACA GCCAGGAGAT GAAATCATCT CAAATGCAGG ATACAATTTC CAAATTCCGA 
GATGGGCATG TGACACTGTT AGTTGCCACA AGCGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGTAACG TTGTTATGCG TTTCGACCTT GCAAAGACGG TGCTGGCATA CATTCAGTCT 
CGTGGCCGGG CAAGAAAGCC TGGATCAGAC TACATACTCA TGGTTGAGAG AGGAAATGTA 
TCTCACGCAG CGTTCCTAAG GAATGCTAGG AACAGTGAGG AGACACTTCG AAAAGAAGCA 
ATAGAAAGGA CTGATCTTAG TCATCTCAAA GATACATCGA GATTAATCTC AATTGATGCT 
GTGCCTGGTA CAGTTTATAA GGTGGAGGCA ACTGGTGCCA TGGTTAGCTT GAATTCCGCG 
GTTGGTCTTG TACATTTCTA CTGCTCTCAG CTTCCTGGTG ACAGGTATGC AATCCTTCGT 
CCTGAGTTTA GCATGGAGAA GCATGAAAAG CCTGGGGGCC ACACGGAATA TTCATGTAGG 
CTTCAGCTTC CTTGCAATGC ACCGTTTGAA ATACTTGAGG GTCCTGTTTG CAGTTCAATG 
CGTCTTGCAC AACAGGCTGT ATGTTTAGCT GCTTGCAAGA AACTGCATGA GATGGGTGCA 
TTTACCGATA TGCTATTACC GGACAAAGGA AGTGGTCAAG ACGCTGAGAA GGCTGACCAA 
GATGATGAAG GTGAGCCTGT TCCTGGAACT GCTAGACATA GAGAGTTCTA TCCTGAAGGT 
GTGGCGGATG TACTTAAGGG AGAATGGGTT TCATCTGGAA AGGAAGTTTG TGAGAGCTCA 
AAGCTATTCC ATTTATACAT GTATAATGTC AGATGTGTAG ATTTTGGCTC TTCAAAAGAT 
CCATTCCTAA GCGAAGTTTC AGAGTTCGCG ATTCTTTTTG GCAATGAGCT GGATGCAGAG 
GTTGTATTAT CGATGTCTAT GGATCTTTAT GTTGCTCGGG CCATGATCAC TAAAGCATCT 
CTTGCTTTCA AGGGATCACT TGATATTACA GAAAACCAGC TATCATCTCT AAAAAAGTTT 
CATGTGAGGT TAATGAGTAT CGTGTTGGAT GTTGATGTTG AACCCTCCAC GACACCATGG 
GATCCTGCAA AGGCCTACCT GTTTGTCCCT GTTACTGACA ATACGTCTAT GGAACCCATA 
AAAGGGATCA ACTGGGAATT GGTTGAAAAG ATTACGAAAA CCACAGCGTG GGACAACCCT 
CTTCAGAGAG CTCGTCCCGA TGTATATCTC GGGACTAATG AGAGAACTCT TGGTGGGGAC 
AGAAGGGAAT ATGGGTTTGG TAAACTTCGT CACAACATTG TATTTGGGCA GAAATCTCAC 
CCAACTTATG GTATTAGAGG AGCTGTTGCA TCCTTCGATG TTGTGAGAGC TTCTGGATTG 
TTACCTGTGA GAGATGCTTT TGAGAAGGAA GTAGAAGAGG ATTTATCAAA AGGAAAATTG 
ATGATGGCTG ATGGGTGCAT GGTTGCAGAA GATCTTATTG GGAAAATAGT GACAGCCGCA 
CATTCCGGGA AGCGGTTTTA CGTAGATTCA ATTTGTTATG ACATGAGTGC AGAAACATCT 
TTCCCTAGGA AAGAGGGATA TCTTGGTCCC CTAGAGTACA ACACGTACGC TGACTATTAC 
AAGCAAAAGT ATGGAGTTGA TTTGAACTGT AAGCAACAAC CTTTGATTAA AGGACGTGGT 
GTTTCGTATT GCAAGAACCT TCTTTCTCCT CGGTTTGAAC AGTCAGGTGA ATCTGAGACA 
GTCCTTGATA AGACATATTA CGTGTTTCTT CCACCTGAAC TATGCGTTGT GCATCCGCTT 
TCGGGTTCAC TTATCCGAGG TGCTCAGAGG TTACCCTCTA TAATGAGAAG AGTTGAGAGC 
ATGTTACTCG CTGTTCAACT CAAAAATTTG ATTAGTTATC CTATTCCCAC ATCAAAGATT 
CTTGAAGCCT TGACTGCCGC CTCGTGCCAG GAAACGTTCT GCTACGAGAG AGCTGAGCTT 
TTAGGAGATG CGTATCTAAA ATGGGTTGTT AGTCGTTTTC TGTTTCTCAA GTATCCTCAA 
AAGCACGAGG GTCAGCTTAC AAGGATGAGG CAACAAATGG TTAGTAATAT GGTTCTTTAT 
CAGTTTGCTC TGGTTAAAGG GCTTCAGTCA TATATCCAGG CGGATCGATT CGCCCCGTCT 
AGGTGGTCTG CTCCTGGTGT GCCTCCGGTT TTCGACGAGG ACACAAAAGA TGGAGGATCT 
TCGTTTTTCG ATGAAGAGCA AAAACCTGTT TCCGAGGAAA ACAGCGATGT GTTTGAAGAT 
GGGGAGATGG AGGATGGTGA ACTAGAGGGT GATTTGAGTT CGTACCGAGT TTTATCTAGC 
AAAACGTTAG CTGATGTTGT TGAGGCTTTG ATTGGTGTTT ATTACGTCGA AGGGGGTAAG 
ATTGCAGCTA ATCATTTGAT GAAATGGATT GGGATTCACG TGGAGGATGA TCCTGATGAA 
GTCGATGGAA CATTGAAAAA TGTTAATGTT CCAGAGAGTG TGCTCAAGAG CATCGACTTT 
GTTGGTCTTG AGAGAGCTCT TAAATATGAG TTTAAAGAGA AAGGTCTTCT TGTTGAAGCT 
ATAACACATG CTTCAAGACC ATCTTCAGGT GTTTCGTGTT ACCAGAGATT GGAATTTGTT 
GGTGACGCGG TCTTGGATCA TCTCATCACA AGACATCTAT TTTTCACATA CACAAGCCTT 
CCTCCTGGTC GGTTAACAGA TCTTCGAGCT GCAGCGGTTA ACAACGAGAA TTTTGCTCGC 
GTTGCGGTTA AACATAAACT CCACTTGTAC CTTCGTCACG GTTCAAGCGC CCTCGAAAAA 
CAGATTCGGG AATTTGTGAA GGAGGTTCAA ACCGAGTCAT CGAAACCGGG GTTTAACTCT 
TTTGGTTTGG GAGACTGCAA AGCACCAAAA GTTCTTGGAG ACATTGTTGA ATCTATTGCA 
GGTGCTATTT TTCTTGATAG TGGAAAAGAT ACAACTGCTG CTTGGAAGGT TTTTCAACCT 
TTGCTTCAGC CCATGGTGAC ACCAGAGACA CTTCCAATGC ATCCGGTGCG AGAGCTACAA 
GAGCGGTGCC AGCAACAAGC AGAAGGGTTA GAATACAAAG CGAGTAGGAG TGGTAACACA 
GCGACTGTGG AAGTTTTCAT CGACGGTGTT CAAGTTGGAG TAGCGCAAAA CCCGCAGAAG 
AAAATGGCTC AAAAGCTAGC TGCGAGGAAC GCACTTGCAG CTTTGAAAGA GAAAGAAATA 
GCAGAATCAA AGGAGAAGCA TATCAACAAC GGTAATGCGG GAGAGGATCA AGGCGAGAAT 
GAGAATGGGA ACAAGAAGAA TGGGCATCAG CCGTTTACGA GACAAACGTT GAATGATATT 
TGTTTGAGGA AGAATTGGCC AATGCCTTCT TACAGATGTG TGAAAGAAGG AGGACCGGCT 
CATGCAAAGA GATTTACGTT TGGGGTAAGA GTTAATACGA GCGATAGAGG ATGGACCGAT 
GAGTGTATTG GCGAGCCAAT GCCGAGTGTT AAGAAAGCTA AGGATTCAGC TGCGGTTCTT 
CTACTTGAGC TTTTAAATAA AACTTTTTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184881.1
CDS	104..5836
Translation	MVMEDEPREATIKPSYWLDACEDISCDLIDDLVSEFDPSSVAVNESTDENGVINDFFGGIDHILDSIKNGGGLPNNGVSDTNSQINEVTVTPQVIAKETVKENGLQKNGGKRDEFSKEEGDKDRKRARVCSYQSERSNLSGRGHVNNSREGDRFMNRKRTRNWDEAGNNKKKRECNNYRRDGRDREVRGYWERDKVGSNELVYRSGTWEADHERDVKKVSGGNRECDVKAEENKSKPEERKEKVVEEQARRYQLDVLEQAKAKNTIAFLETGAGKTLIAILLIKSVHKDLMSQNRKMLSVFLVPKVPLVYQQAEVIRNQTCFQVGHYCGEMGQDFWDSRRWQREFESKQVLVMTAQILLNILRHSIIRMETIDLLILDECHHAVKKHPYSLVMSEFYHTTPKDKRPAIFGMTASPVNLKGVSSQVDCAIKIRNLETKLDSTVCTIKDRKELEKHVPMPSEIVVEYDKAATMWSLHETIKQMIAAVEEAAQASSRKSKWQFMGARDAGAKDELRQVYGVSERTESDGAANLIHKLRAINYTLAELGQWCAYKVGQSFLSALQSDERVNFQVDVKFQESYLSEVVSLLQCELLEGAAAEKVAAEVGKPENGNAHDEMEEGELPDDPVVSGGEHVDEVIGAAVADGKVTPKVQSLIKLLLKYQHTADFRAIVFVERVVAALVLPKVFAELPSLSFIRCASMIGHNNSQEMKSSQMQDTISKFRDGHVTLLVATSVAEEGLDIRQCNVVMRFDLAKTVLAYIQSRGRARKPGSDYILMVERGNVSHAAFLRNARNSEETLRKEAIERTDLSHLKDTSRLISIDAVPGTVYKVEATGAMVSLNSAVGLVHFYCSQLPGDRYAILRPEFSMEKHEKPGGHTEYSCRLQLPCNAPFEILEGPVCSSMRLAQQAVCLAACKKLHEMGAFTDMLLPDKGSGQDAEKADQDDEGEPVPGTARHREFYPEGVADVLKGEWVSSGKEVCESSKLFHLYMYNVRCVDFGSSKDPFLSEVSEFAILFGNELDAEVVLSMSMDLYVARAMITKASLAFKGSLDITENQLSSLKKFHVRLMSIVLDVDVEPSTTPWDPAKAYLFVPVTDNTSMEPIKGINWELVEKITKTTAWDNPLQRARPDVYLGTNERTLGGDRREYGFGKLRHNIVFGQKSHPTYGIRGAVASFDVVRASGLLPVRDAFEKEVEEDLSKGKLMMADGCMVAEDLIGKIVTAAHSGKRFYVDSICYDMSAETSFPRKEGYLGPLEYNTYADYYKQKYGVDLNCKQQPLIKGRGVSYCKNLLSPRFEQSGESETVLDKTYYVFLPPELCVVHPLSGSLIRGAQRLPSIMRRVESMLLAVQLKNLISYPIPTSKILEALTAASCQETFCYERAELLGDAYLKWVVSRFLFLKYPQKHEGQLTRMRQQMVSNMVLYQFALVKGLQSYIQADRFAPSRWSAPGVPPVFDEDTKDGGSSFFDEEQKPVSEENSDVFEDGEMEDGELEGDLSSYRVLSSKTLADVVEALIGVYYVEGGKIAANHLMKWIGIHVEDDPDEVDGTLKNVNVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEKEIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGGPAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNKTFS

Target ORF information:

RefSeq Version	NM_001197952.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana dicer-like 1 (DCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197952.1

ORF Insert Sequence:

ATGGTAATGG AGGATGAGCC TAGAGAAGCC ACAATAAAGC CTTCTTATTG GCTAGATGCT 
TGCGAGGACA TCTCTTGTGA TCTTATCGAT GATCTCGTGT CTGAATTTGA TCCTTCCTCT 
GTTGCTGTCA ATGAATCCAC TGATGAAAAC GGCGTCATCA ATGATTTTTT CGGTGGGATT 
GATCACATTT TAGATAGTAT CAAGAACGGT GGAGGCTTAC CAAACAATGG CGTTTCTGAT 
ACCAATTCTC AAATCAACGA GGTTACTGTA ACTCCTCAGG TTATTGCTAA GGAGACAGTG 
AAGGAGAATG GGTTGCAAAA GAATGGCGGT AAGAGAGACG AATTCTCGAA AGAGGAAGGA 
GACAAGGATA GGAAGAGAGC TAGGGTTTGT AGTTATCAGA GTGAAAGGAG TAACCTTTCA 
GGTAGAGGGC ATGTTAATAA TTCTAGGGAG GGAGATAGGT TTATGAATAG GAAACGTACT 
CGTAATTGGG ACGAGGCGGG TAACAATAAG AAGAAAAGGG AATGTAACAA TTACAGAAGA 
GATGGTAGAG ATAGAGAAGT TAGGGGTTAT TGGGAGAGGG ATAAAGTTGG TTCCAATGAG 
TTGGTTTATA GGTCAGGGAC TTGGGAAGCT GATCATGAAA GAGATGTTAA GAAAGTGAGT 
GGTGGAAACC GCGAATGCGA TGTCAAGGCA GAGGAGAACA AGAGTAAGCC TGAAGAACGT 
AAAGAGAAGG TTGTGGAAGA GCAAGCAAGG CGATACCAGT TGGATGTTCT TGAACAAGCT 
AAAGCGAAAA ACACGATTGC TTTCCTTGAG ACCGGTGCTG GAAAGACACT TATCGCGATT 
CTTCTTATTA AAAGTGTTCA TAAGGATCTG ATGAGCCAGA ACAGAAAAAT GCTCTCGGTG 
TTCTTGGTTC CCAAAGTGCC TTTGGTTTAT CAGCAAGCAG AAGTGATCCG TAATCAAACT 
TGTTTTCAAG TTGGACATTA TTGTGGTGAG ATGGGACAGG ACTTTTGGGA TTCTCGAAGG 
TGGCAACGAG AGTTTGAGTC TAAGCAGGTT CTAGTTATGA CAGCACAAAT TCTGTTGAAT 
ATACTGAGAC ACAGTATCAT TAGAATGGAA ACAATTGATC TTCTTATTCT CGACGAGTGT 
CACCACGCTG TCAAGAAACA TCCATACTCT TTAGTGATGT CAGAGTTTTA CCATACAACT 
CCTAAAGATA AAAGACCTGC CATCTTTGGA ATGACTGCTT CGCCTGTTAA TTTAAAGGGT 
GTTTCAAGCC AAGTAGATTG TGCGATAAAG ATACGTAACC TCGAGACCAA GTTGGATTCT 
ACGGTTTGTA CTATAAAAGA TCGAAAAGAA TTAGAGAAAC ATGTGCCTAT GCCTTCAGAG 
ATAGTCGTCG AGTATGACAA AGCTGCTACT ATGTGGTCTC TTCATGAGAC AATAAAGCAA 
ATGATTGCAG CTGTTGAAGA AGCGGCACAA GCAAGTTCAA GGAAAAGCAA GTGGCAATTT 
ATGGGGGCTA GGGATGCTGG AGCAAAGGAT GAATTGAGAC AGGTTTATGG CGTCTCTGAA 
AGAACGGAGA GCGATGGTGC TGCCAATTTG ATTCATAAAC TTAGAGCTAT CAATTATACT 
CTTGCTGAAT TGGGTCAATG GTGTGCTTAC AAGGTGGGAC AATCATTCTT GTCTGCTTTG 
CAAAGTGATG AGAGGGTGAA TTTCCAAGTC GACGTGAAGT TTCAAGAATC ATACCTCAGT 
GAGGTGGTGT CACTCTTGCA ATGTGAGCTT CTGGAAGGCG CTGCTGCTGA AAAAGTCGCG 
GCGGAAGTTG GCAAACCAGA AAATGGTAAT GCACATGACG AGATGGAGGA GGGAGAGCTC 
CCTGATGATC CTGTGGTCTC GGGAGGGGAG CACGTTGATG AAGTAATAGG CGCCGCAGTG 
GCTGATGGGA AAGTTACTCC AAAAGTACAA TCATTGATCA AACTACTCCT CAAATATCAG 
CACACAGCTG ATTTTCGAGC TATTGTTTTC GTTGAGAGGG TGGTTGCTGC TTTGGTTCTT 
CCTAAGGTTT TTGCGGAGCT GCCTTCGCTT AGTTTTATAC GGTGTGCCAG CATGATTGGA 
CACAATAACA GCCAGGAGAT GAAATCATCT CAAATGCAGG ATACAATTTC CAAATTCCGA 
GATGGGCATG TGACACTGTT AGTTGCCACA AGCGTTGCTG AGGAAGGACT TGATATTAGG 
CAATGTAACG TTGTTATGCG TTTCGACCTT GCAAAGACGG TGCTGGCATA CATTCAGTCT 
CGTGGCCGGG CAAGAAAGCC TGGATCAGAC TACATACTCA TGGTTGAGAG AGGAAATGTA 
TCTCACGCAG CGTTCCTAAG GAATGCTAGG AACAGTGAGG AGACACTTCG AAAAGAAGCA 
ATAGAAAGGA CTGATCTTAG TCATCTCAAA GATACATCGA GATTAATCTC AATTGATGCT 
GTGCCTGGTA CAGTTTATAA GGTGGAGGCA ACTGGTGCCA TGGTTAGCTT GAATTCCGCG 
GTTGGTCTTG TACATTTCTA CTGCTCTCAG CTTCCTGGTG ACAGGTATGC AATCCTTCGT 
CCTGAGTTTA GCATGGAGAA GCATGAAAAG CCTGGGGGCC ACACGGAATA TTCATGTAGG 
CTTCAGCTTC CTTGCAATGC ACCGTTTGAA ATACTTGAGG GTCCTGTTTG CAGTTCAATG 
CGTCTTGCAC AACAGGCTGT ATGTTTAGCT GCTTGCAAGA AACTGCATGA GATGGGTGCA 
TTTACCGATA TGCTATTACC GGACAAAGGA AGTGGTCAAG ACGCTGAGAA GGCTGACCAA 
GATGATGAAG GTGAGCCTGT TCCTGGAACT GCTAGACATA GAGAGTTCTA TCCTGAAGGT 
GTGGCGGATG TACTTAAGGG AGAATGGGTT TCATCTGGAA AGGAAGTTTG TGAGAGCTCA 
AAGCTATTCC ATTTATACAT GTATAATGTC AGATGTGTAG ATTTTGGCTC TTCAAAAGAT 
CCATTCCTAA GCGAAGTTTC AGAGTTCGCG ATTCTTTTTG GCAATGAGCT GGATGCAGAG 
GTTGTATTAT CGATGTCTAT GGATCTTTAT GTTGCTCGGG CCATGATCAC TAAAGCATCT 
CTTGCTTTCA AGGGATCACT TGATATTACA GAAAACCAGC TATCATCTCT AAAAAAGTTT 
CATGTGAGGT TAATGAGTAT CGTGTTGGAT GTTGATGTTG AACCCTCCAC GACACCATGG 
GATCCTGCAA AGGCCTACCT GTTTGTCCCT GTTACTGACA ATACGTCTAT GGAACCCATA 
AAAGGGATCA ACTGGGAATT GGTTGAAAAG ATTACGAAAA CCACAGCGTG GGACAACCCT 
CTTCAGAGAG CTCGTCCCGA TGTATATCTC GGGACTAATG AGAGAACTCT TGGTGGGGAC 
AGAAGGGAAT ATGGGTTTGG TAAACTTCGT CACAACATTG TATTTGGGCA GAAATCTCAC 
CCAACTTATG GTATTAGAGG AGCTGTTGCA TCCTTCGATG TTGTGAGAGC TTCTGGATTG 
TTACCTGTGA GAGATGCTTT TGAGAAGGAA GTAGAAGAGG ATTTATCAAA AGGAAAATTG 
ATGATGGCTG ATGGGTGCAT GGTTGCAGAA GATCTTATTG GGAAAATAGT GACAGCCGCA 
CATTCCGGGA AGCGGTTTTA CGTAGATTCA ATTTGTTATG ACATGAGTGC AGAAACATCT 
TTCCCTAGGA AAGAGGGATA TCTTGGTCCC CTAGAGTACA ACACGTACGC TGACTATTAC 
AAGCAAAAGT ATGGAGTTGA TTTGAACTGT AAGCAACAAC CTTTGATTAA AGGACGTGGT 
GTTTCGTATT GCAAGAACCT TCTTTCTCCT CGGTTTGAAC AGTCAGGTGA ATCTGAGACA 
GTCCTTGATA AGACATATTA CGTGTTTCTT CCACCTGAAC TATGCGTTGT GCATCCGCTT 
TCGGGTTCAC TTATCCGAGG TGCTCAGAGG TTACCCTCTA TAATGAGAAG AGTTGAGAGC 
ATGTTACTCG CTGTTCAACT CAAAAATTTG ATTAGTTATC CTATTCCCAC ATCAAAGATT 
CTTGAAGCCT TGACTGCCGC CTCGTGCCAG GAAACGTTCT GCTACGAGAG AGCTGAGCTT 
TTAGGAGATG CGTATCTAAA ATGGGTTGTT AGTCGTTTTC TGTTTCTCAA GTATCCTCAA 
AAGCACGAGG GTCAGCTTAC AAGGATGAGG CAACAAATGG TTAGTAATAT GGTTCTTTAT 
CAGTTTGCTC TGGTTAAAGG GCTTCAGTCA TATATCCAGG CGGATCGATT CGCCCCGTCT 
AGGTGGTCTG CTCCTGGTGT GCCTCCGGTT TTCGACGAGG ACACAAAAGA TGGAGGATCT 
TCGTTTTTCG ATGAAGAGCA AAAACCTGTT TCCGAGGAAA ACAGCGATGT GTTTGAAGAT 
GGGGAGATGG AGGATGGTGA ACTAGAGGGT GATTTGAGTT CGTACCGAGT TTTATCTAGC 
AAAACGTTAG CTGATGTTGT TGAGGCTTTG ATTGGTGTTT ATTACGTCGA AGGGGGTAAG 
ATTGCAGCTA ATCATTTGAT GAAATGGATT GGGATTCACG TGGAGGATGA TCCTGATGAA 
GTCGATGGAA CATTGAAAAA TGTTAATGTT CCAGAGAGTG TGCTCAAGAG CATCGACTTT 
GTTGGTCTTG AGAGAGCTCT TAAATATGAG TTTAAAGAGA AAGGTCTTCT TGTTGAAGCT 
ATAACACATG CTTCAAGACC ATCTTCAGGT GTTTCGTGTT ACCAGAGATT GGAATTTGTT 
GGTGACGCGG TCTTGGATCA TCTCATCACA AGACATCTAT TTTTCACATA CACAAGCCTT 
CCTCCTGGTC GGTTAACAGA TCTTCGAGCT GCAGCGGTTA ACAACGAGAA TTTTGCTCGC 
GTTGCGGTTA AACATAAACT CCACTTGTAC CTTCGTCACG GTTCAAGCGC CCTCGAAAAA 
CAGATTCGGG AATTTGTGAA GGAGGTTCAA ACCGAGTCAT CGAAACCGGG GTTTAACTCT 
TTTGGTTTGG GAGACTGCAA AGCACCAAAA GTTCTTGGAG ACATTGTTGA ATCTATTGCA 
GGTGCTATTT TTCTTGATAG TGGAAAAGAT ACAACTGCTG CTTGGAAGGT TTTTCAACCT 
TTGCTTCAGC CCATGGTGAC ACCAGAGACA CTTCCAATGC ATCCGGTGCG AGAGCTACAA 
GAGCGGTGCC AGCAACAAGC AGAAGGGTTA GAATACAAAG CGAGTAGGAG TGGTAACACA 
GCGACTGTGG AAGTTTTCAT CGACGGTGTT CAAGTTGGAG TAGCGCAAAA CCCGCAGAAG 
AAAATGGCTC AAAAGCTAGC TGCGAGGAAC GCACTTGCAG CTTTGAAAGA GAAAGAAATA 
GCAGAATCAA AGGAGAAGCA TATCAACAAC GGTAATGCGG GAGAGGATCA AGGCGAGAAT 
GAGAATGGGA ACAAGAAGAA TGGGCATCAG CCGTTTACGA GACAAACGTT GAATGATATT 
TGTTTGAGGA AGAATTGGCC AATGCCTTCT TACAGATGTG TGAAAGAAGG AGGACCGGCT 
CATGCAAAGA GATTTACGTT TGGGGTAAGA GTTAATACGA GCGATAGAGG ATGGACCGAT 
GAGTGTATTG GCGAGCCAAT GCCGAGTGTT AAGAAAGCTA AGGATTCAGC TGCGGTTCTT 
CTACTTGAGC TTTTAAATAA AACTTTTTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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DCL1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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