AT1G23230 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT1G23230
Entrez Gene ID	838932
Full Name	mediator of RNA polymerase II transcription subunit
Synonyms	F26F24.8,F26F24_8
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001198142.2	NP_001185071.1	mediator of RNA polymerase II transcription subunit
NM_102172.2	NP_173737.1	mediator of RNA polymerase II transcription subunit
NM_001332594.1	NP_001322591.1	mediator of RNA polymerase II transcription subunit

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT1G23230	NP_173737.1
Oryza sativa (rice)	Os02g0732700	NP_001048029.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT1G23230 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT1G23230 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb33780	NM_001332594.1 Latest version!	Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAb24312	NM_102172.2 Latest version!	Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OAb31143	NM_001198142.2 Latest version!	Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT1G23230 ( NM_001332594.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001322591.1 Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), mRNA.

Price & Availability↑

CloneID	OAb33780
Clone ID Related Accession (Same CDS sequence)	NM_001332594.1
Accession Version	NM_001332594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	mediator of RNA polymerase II transcription subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGATCAAT CGCAGCGAAC AGTCGCTGCA ACGCCATCGT CTTCTCGATC GTACCAATTC 
CACCCAGCTC GTGCTGCAAT TATCGATCTT TTCAATCTCT ACTTAGGGAG AGGAAGCCGT 
CAAAAGCCTG ATGAGTCCCT CCGAGATCCC CCGAACAAAT CACAGAAGCG TGTTCATGCT 
CCTAATAGAG ACTTACCACC AAGAAACGAG CAATTCCTTT TGGATTTTGA GCTGCTTCAG 
AGCCAATTCA ATGATCCAGA GCAATTGAGG ACGATTACTG AGTCAGTTTT GATATCTCTG 
GTGGTGCAAT GTAGCAATCA TGCGCCTCGT GCTGAGTTTC TACTCTTTGC TTTGCGTACA 
TTGTGTAGGA TTAGTTATAT CAATTGGGAT ACTTTTTTAC CATCCCTTCT CTCTTCCGTT 
TCCGCTGCTG AGGCGTCGTT AAGCCAAGGT GTTCAAGCAG CAGCGGCAAC GGCTGGTTCT 
TCTGCTACTT CTTCGCAGTC TGTAGTTCCT GTGTCTGTGA ATCCAACGTC ATTGCTGCCT 
TCAGCTCATG GTATTGGTTC TCCATCAGCT TCAGAGGTGA AATCTGTGGA GAATGGGCAG 
CAGATTGCAA GAGCTGGCCA GATTGTAAGA GAGAATGCTA TGAGAAATAG TCAACGGATT 
AGAGCTGCTG CTGTAAACAG TCTGCGTCAG CTCAGTTGCA AGATTATCTT GATTGGCGTT 
GAATCTAGTC TCAAACCCGT TACTCATGCC GAGATTTTCC AGTACATGAT GAATTGGCTT 
GTTAACTGGG ACAGGAGAGA TCTGGGAACT GAGGACTCTG TAGGAAAGTC GTGGAGATCT 
GAGAAGACTT TAGCTGAATG GTTGCGGAGT TGTTTGGATG TGATCTGGCT GTTGGTAGAA 
GAGGGTGAAT CTCGAATTCC GTTTTATGAG TTATTGCGCA GTGGTCTACA GTTTATAGAG 
AACATACCTG ATGATGAAGC CTTGTTCACT CTTATCATGG AGATACACCG GAGGCGAGAT 
GCGATGGCCA TGCATATGCT CATGTTGGAC CAACATCTTC ACTGTCCATC ATTTGGTACC 
CATCGTATAG TCTCCCAAAT AACTGCCAAT GTTCCACCCG AAGCTGTGCC ACACTTACGA 
CATTCACCAA TTACATATCC AAGTGTGCTT GGGGAACCTT TATATGGAGA GGATCTGGCG 
ATGTCTATTC CAAAAGGAAG CCTGGATTGG GAGAGAGCAG TGCGTTGCAT TAGACATGCT 
ATTCGCACTA CACCGTCACC GGACTGGTGG AAGCGTGTTC TTGTTGTGGC CCCTTGTTAT 
AGACCATCTA CACAAGCAGG GCCTATCCCT GGTGCTGTAT TCACTTCTGA CATGATTTGT 
GAGGCAATCA TTGACAGAAT TGTTGAGCTT CTTAAGCTGA CCAACTCAGA TGCAAATTGC 
TGGCAAGAGT GGCTGGTTTT CTCGGATATA TTCTTCTTTC TAATCAAAAG TGGGTGTACT 
GATTTTGTTG ATTTCATTGA CAAGTTGGTT TTACGCCTTA ACGGTGTTGA TAACCATATT 
CTTCGGACGA ATCATGTGAC TTGGCTGCTT GCACAAATAA TACGCGTGGA GCTTGTGATG 
ACTGCTTTGA ACTCAGATGC TAAAAAGGTG GAGACTACTA GGAAGATCTT ATCATTTCAC 
AGGGAAGACA GAAATTCTGA TCCCAACAAT CCTCAGAGTG TGTTGCTTGA CTTCGTAAGC 
AGTTGTCAGA ATCTACGGAT TTGGTCACTA AGCACAACAA CTAGGGCATA CCTAAACAAT 
GAACAGCTGT TGAAGGGAAA ACAGATCGAT GAGTGGTGGA GAAGCAAAGG AGAGCGGATG 
ATGGATTATA TGAATATGGA TGATAGGTCG ATTGGCATGT TTTGGGTTGT TTCCTACACC 
ATGGCACAAC CAGCATGTGA AACAGTTATA AATTGGCTAT CTTCAGCTGG CATGGCTGAG 
TTACCGGGAT TACAGCCAAA CGACCGAGTA ATGATGACGC AGGAAGTTAC CCCGTTACCT 
ATGTCATTGC TATCTGGCTT TTCAATGAAT TTGTGCTTGA AATTGGCCCT TCAGATGGAA 
GAAGCTTTGT TTGTTAGCCA GGTTGTTCCT AGCATTGCAA TGGTTGAGAC ATACACAAGA 
TTGCTTCTGA TTTCCCCTCA TTCGATGTTC CGTTCACATT TCAGTCAATT GGCCCAGCGA 
AATGCTTCTC TGCTAAGCAA GCCTGGAGTG ACGTTACTTG TTCTTGAGAT TCTGAACTAT 
CGGTTGCTGC CACTCTACAG ATACCAAGGA AAGAGTAAAA CTTTAATGTA TGATGTTACT 
AAAATAATAT CTGCATTGAA AGGAAAACGT GGTGACCACC GTATATTCAG ACTAGCTGAA 
AACTTATGCA TGAATCTCAT TTTATCACTC AGAGACTTTT TCTCTGTGAA ACGGGAGGGA 
AAAGGGCCAA CTGAATTCAC TGAGACGTTA AATCGCATAA CCATCATGAC TCTTGCTATC 
ACTATCAAAA CACGTGGTAT CGCAGACCCT GATCACATGG TTTATCTGCA AACCATGTTG 
GAACAAATAC TCGCGACGAG TCAGCATACA TGGTCAGAGA AGACGATGCG GCATTTCCCA 
TCCCTTCTCC GTGAAACTTT GAAAGGACGG GTAGACAAGA GGGGCCTATC AATTCAGGCA 
TGGCAACAGG CTGAAACAAC TGTGATAAAC CAGTGCACTC AACTTCTCTC ACCATCTGCT 
GAACCCGCTT ACGTTTCAAC ATACCTCAGT CACAGTTTTC CTCAACACCG TCAGTATCTA 
TGTGCTGGTG CTTGTCTGCT GATGCAAGGC CACGCTGAAA ACATCAACAG CACAAATCTG 
GCACGAGTTC TGAGAGAAGT TTCTCCTGAA GAAGTCACTG CTAACATATA CACTTTGGTC 
GATGTTTTGC TTCACCACGT TCATGTAGAC CTTCAGCAAG GACAATCTCT AGAGGCAGTT 
CTAGACAAGG CAGGCGCAAA TCTTGCATTT TTCTTCTGGA CACATGAAAT GCTTCCTCTC 
GACATTTTTC TTTTGGCGCT CATTGACCGT GATGATGATC CTCACGCCTT GATAATTGCA 
ATGAGCCTAC TTAAAACGCC AGACCTTCTG CTGAGGATAA AAAACTACTG CCAAAACCGT 
GGGTCTCCTG AGCACTGGCT TGTCACACAG GTGTTCAAAC GCAATGAACT CCAGAAGGCC 
CTGGGTAACC ATCTTTCTTG GAAGGACAGG TATCCAACGT TCTTTGATGA TATTGCAGCG 
CGTTTGCTTC CAGTTATCCC TCTAGTGCTT TACAGGCTCA TCGAGAACAA TGCGATGGAG 
CAAGCTGATA ATCTTTTGCT TGCACACTCG CATTTTCTAG CGTATCATCC TCTCAGATTC 
ACGTTTGTCC GTGATATACT AGCCTATTTC TATGGCCATC TTCCTGGGAA ACTTGTTCTG 
CGCATGCTCA AAGTACTTGA TCTCAGCAAG ATTCCATTCT CTGAATCATT CCCGCAGTAC 
ATTAGCCCTA CCGGTGCACC TGTATGCCCA CCTCTAGATT ACTTTGCCTC TCTTCTGCTT 
AATCTCGTAA ACAATGTTAT ACCTCCACTT AGCAGCAGCA GCAACTGCAG CTCGAGGTCT 
GGCTCAATGG CAGACATCTT AAACAGCTCA GCAAGACCTC CTCATGGAAA AACTCCAGGA 
ACATCTCAGC CTGGACCAGC AAACGCCTCT GAGGGCCAGA AAGCATTCTA TCAGATCCAG 
GACCCAGGAA CTTACACGCA ACTGGTTCTT GAGACAGCCG TGATCGAGAT CCTCTCGCTT 
CCTGTCTCTG CTGCTCAGAT TGTCTCTTCG CTTGTCCAGA TAATTGTCAA CATACAATCG 
ACTCTGATTC AATCTGGAAA CGGGTTCCAC GGCGCTGCCA ACGGGGTGGG ACAAGGGTCG 
GTACTGCCAA CGTCTCCCTC TGGAGGGAGC ACAGACTCCA TGAGCGCTAG CCGGTCCACT 
TGTCTGATTC CTGGTATCAA CACAGCGAGC TTTGTCTCTA GAAGCGGTTA TACATGTCAG 
CAACTGTCGT GTCTGTTGAT TCAAGCTTGT GGACTTCTGT TGGCTCAGCT CCCTCCAGAT 
TTTCACGTAC AGCTTTACTT GGAGGCAGCA CGTGTGACAA GGGAAACATG GTGGCTTAAG 
GACGGGAAGA GATCACAAGG TGAACTAGAC TCAGCTGTTG GATACGCTTT GATGGATCCT 
ACATGGGCTG CTCAGGATAA CACCTCGACC GCCATAGGAA ATATTGTCGC CTTGCTTCAT 
GCTTTCTTCA GCAATCTCCC ACAAGAATGG CTTGATGGCA CGAATGCTAT CATCACGAAC 
CTCCGGCCTG TGACATCTGT GGCAATGCTC AGAGTTGTAT TCCGTATCAT GGGGCCACTG 
CTTCCGAGGC TTGCCAGTAC ACATACTCTC TTTAACAAGA CGCTAATGCT TCTCTTAAGC 
GCACTGGTCG ATGTTTTTGG AAAGACCGCA CAGACAACAG CCCCTGTTGA AGCATCCCAG 
ATTGCAGATC TTATTGATTT CCTACACCAT ATTATTCACT ACGAGGGACA AGGAGGAGCT 
GTTCAAACTA GCAGCAAGCC GAGACCGGAC ATCTTGGCGT TAATCGGAAG AGCAGCAGAG 
ACTCTCCGAC CAGATGTACA ACACCTTCTC GCTCACCTCA AAACCAACCC GAATTCTTCC 
ATATACGCAG CTGCTCATCA GCAGAATACT GCAAAGACTA ACACCTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001322591.1
CDS	64..4854
Translation	MDQSQRTVAATPSSSRSYQFHPARAAIIDLFNLYLGRGSRQKPDESLRDPPNKSQKRVHAPNRDLPPRNEQFLLDFELLQSQFNDPEQLRTITESVLISLVVQCSNHAPRAEFLLFALRTLCRISYINWDTFLPSLLSSVSAAEASLSQGVQAAAATAGSSATSSQSVVPVSVNPTSLLPSAHGIGSPSASEVKSVENGQQIARAGQIVRENAMRNSQRIRAAAVNSLRQLSCKIILIGVESSLKPVTHAEIFQYMMNWLVNWDRRDLGTEDSVGKSWRSEKTLAEWLRSCLDVIWLLVEEGESRIPFYELLRSGLQFIENIPDDEALFTLIMEIHRRRDAMAMHMLMLDQHLHCPSFGTHRIVSQITANVPPEAVPHLRHSPITYPSVLGEPLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSPDWWKRVLVVAPCYRPSTQAGPIPGAVFTSDMICEAIIDRIVELLKLTNSDANCWQEWLVFSDIFFFLIKSGCTDFVDFIDKLVLRLNGVDNHILRTNHVTWLLAQIIRVELVMTALNSDAKKVETTRKILSFHREDRNSDPNNPQSVLLDFVSSCQNLRIWSLSTTTRAYLNNEQLLKGKQIDEWWRSKGERMMDYMNMDDRSIGMFWVVSYTMAQPACETVINWLSSAGMAELPGLQPNDRVMMTQEVTPLPMSLLSGFSMNLCLKLALQMEEALFVSQVVPSIAMVETYTRLLLISPHSMFRSHFSQLAQRNASLLSKPGVTLLVLEILNYRLLPLYRYQGKSKTLMYDVTKIISALKGKRGDHRIFRLAENLCMNLILSLRDFFSVKREGKGPTEFTETLNRITIMTLAITIKTRGIADPDHMVYLQTMLEQILATSQHTWSEKTMRHFPSLLRETLKGRVDKRGLSIQAWQQAETTVINQCTQLLSPSAEPAYVSTYLSHSFPQHRQYLCAGACLLMQGHAENINSTNLARVLREVSPEEVTANIYTLVDVLLHHVHVDLQQGQSLEAVLDKAGANLAFFFWTHEMLPLDIFLLALIDRDDDPHALIIAMSLLKTPDLLLRIKNYCQNRGSPEHWLVTQVFKRNELQKALGNHLSWKDRYPTFFDDIAARLLPVIPLVLYRLIENNAMEQADNLLLAHSHFLAYHPLRFTFVRDILAYFYGHLPGKLVLRMLKVLDLSKIPFSESFPQYISPTGAPVCPPLDYFASLLLNLVNNVIPPLSSSSNCSSRSGSMADILNSSARPPHGKTPGTSQPGPANASEGQKAFYQIQDPGTYTQLVLETAVIEILSLPVSAAQIVSSLVQIIVNIQSTLIQSGNGFHGAANGVGQGSVLPTSPSGGSTDSMSASRSTCLIPGINTASFVSRSGYTCQQLSCLLIQACGLLLAQLPPDFHVQLYLEAARVTRETWWLKDGKRSQGELDSAVGYALMDPTWAAQDNTSTAIGNIVALLHAFFSNLPQEWLDGTNAIITNLRPVTSVAMLRVVFRIMGPLLPRLASTHTLFNKTLMLLLSALVDVFGKTAQTTAPVEASQIADLIDFLHHIIHYEGQGGAVQTSSKPRPDILALIGRAAETLRPDVQHLLAHLKTNPNSSIYAAAHQQNTAKTNTS

Target ORF information:

RefSeq Version	NM_001332594.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001332594.1

ORF Insert Sequence:

ATGGATCAAT CGCAGCGAAC AGTCGCTGCA ACGCCATCGT CTTCTCGATC GTACCAATTC 
CACCCAGCTC GTGCTGCAAT TATCGATCTT TTCAATCTCT ACTTAGGGAG AGGAAGCCGT 
CAAAAGCCTG ATGAGTCCCT CCGAGATCCC CCGAACAAAT CACAGAAGCG TGTTCATGCT 
CCTAATAGAG ACTTACCACC AAGAAACGAG CAATTCCTTT TGGATTTTGA GCTGCTTCAG 
AGCCAATTCA ATGATCCAGA GCAATTGAGG ACGATTACTG AGTCAGTTTT GATATCTCTG 
GTGGTGCAAT GTAGCAATCA TGCGCCTCGT GCTGAGTTTC TACTCTTTGC TTTGCGTACA 
TTGTGTAGGA TTAGTTATAT CAATTGGGAT ACTTTTTTAC CATCCCTTCT CTCTTCCGTT 
TCCGCTGCTG AGGCGTCGTT AAGCCAAGGT GTTCAAGCAG CAGCGGCAAC GGCTGGTTCT 
TCTGCTACTT CTTCGCAGTC TGTAGTTCCT GTGTCTGTGA ATCCAACGTC ATTGCTGCCT 
TCAGCTCATG GTATTGGTTC TCCATCAGCT TCAGAGGTGA AATCTGTGGA GAATGGGCAG 
CAGATTGCAA GAGCTGGCCA GATTGTAAGA GAGAATGCTA TGAGAAATAG TCAACGGATT 
AGAGCTGCTG CTGTAAACAG TCTGCGTCAG CTCAGTTGCA AGATTATCTT GATTGGCGTT 
GAATCTAGTC TCAAACCCGT TACTCATGCC GAGATTTTCC AGTACATGAT GAATTGGCTT 
GTTAACTGGG ACAGGAGAGA TCTGGGAACT GAGGACTCTG TAGGAAAGTC GTGGAGATCT 
GAGAAGACTT TAGCTGAATG GTTGCGGAGT TGTTTGGATG TGATCTGGCT GTTGGTAGAA 
GAGGGTGAAT CTCGAATTCC GTTTTATGAG TTATTGCGCA GTGGTCTACA GTTTATAGAG 
AACATACCTG ATGATGAAGC CTTGTTCACT CTTATCATGG AGATACACCG GAGGCGAGAT 
GCGATGGCCA TGCATATGCT CATGTTGGAC CAACATCTTC ACTGTCCATC ATTTGGTACC 
CATCGTATAG TCTCCCAAAT AACTGCCAAT GTTCCACCCG AAGCTGTGCC ACACTTACGA 
CATTCACCAA TTACATATCC AAGTGTGCTT GGGGAACCTT TATATGGAGA GGATCTGGCG 
ATGTCTATTC CAAAAGGAAG CCTGGATTGG GAGAGAGCAG TGCGTTGCAT TAGACATGCT 
ATTCGCACTA CACCGTCACC GGACTGGTGG AAGCGTGTTC TTGTTGTGGC CCCTTGTTAT 
AGACCATCTA CACAAGCAGG GCCTATCCCT GGTGCTGTAT TCACTTCTGA CATGATTTGT 
GAGGCAATCA TTGACAGAAT TGTTGAGCTT CTTAAGCTGA CCAACTCAGA TGCAAATTGC 
TGGCAAGAGT GGCTGGTTTT CTCGGATATA TTCTTCTTTC TAATCAAAAG TGGGTGTACT 
GATTTTGTTG ATTTCATTGA CAAGTTGGTT TTACGCCTTA ACGGTGTTGA TAACCATATT 
CTTCGGACGA ATCATGTGAC TTGGCTGCTT GCACAAATAA TACGCGTGGA GCTTGTGATG 
ACTGCTTTGA ACTCAGATGC TAAAAAGGTG GAGACTACTA GGAAGATCTT ATCATTTCAC 
AGGGAAGACA GAAATTCTGA TCCCAACAAT CCTCAGAGTG TGTTGCTTGA CTTCGTAAGC 
AGTTGTCAGA ATCTACGGAT TTGGTCACTA AGCACAACAA CTAGGGCATA CCTAAACAAT 
GAACAGCTGT TGAAGGGAAA ACAGATCGAT GAGTGGTGGA GAAGCAAAGG AGAGCGGATG 
ATGGATTATA TGAATATGGA TGATAGGTCG ATTGGCATGT TTTGGGTTGT TTCCTACACC 
ATGGCACAAC CAGCATGTGA AACAGTTATA AATTGGCTAT CTTCAGCTGG CATGGCTGAG 
TTACCGGGAT TACAGCCAAA CGACCGAGTA ATGATGACGC AGGAAGTTAC CCCGTTACCT 
ATGTCATTGC TATCTGGCTT TTCAATGAAT TTGTGCTTGA AATTGGCCCT TCAGATGGAA 
GAAGCTTTGT TTGTTAGCCA GGTTGTTCCT AGCATTGCAA TGGTTGAGAC ATACACAAGA 
TTGCTTCTGA TTTCCCCTCA TTCGATGTTC CGTTCACATT TCAGTCAATT GGCCCAGCGA 
AATGCTTCTC TGCTAAGCAA GCCTGGAGTG ACGTTACTTG TTCTTGAGAT TCTGAACTAT 
CGGTTGCTGC CACTCTACAG ATACCAAGGA AAGAGTAAAA CTTTAATGTA TGATGTTACT 
AAAATAATAT CTGCATTGAA AGGAAAACGT GGTGACCACC GTATATTCAG ACTAGCTGAA 
AACTTATGCA TGAATCTCAT TTTATCACTC AGAGACTTTT TCTCTGTGAA ACGGGAGGGA 
AAAGGGCCAA CTGAATTCAC TGAGACGTTA AATCGCATAA CCATCATGAC TCTTGCTATC 
ACTATCAAAA CACGTGGTAT CGCAGACCCT GATCACATGG TTTATCTGCA AACCATGTTG 
GAACAAATAC TCGCGACGAG TCAGCATACA TGGTCAGAGA AGACGATGCG GCATTTCCCA 
TCCCTTCTCC GTGAAACTTT GAAAGGACGG GTAGACAAGA GGGGCCTATC AATTCAGGCA 
TGGCAACAGG CTGAAACAAC TGTGATAAAC CAGTGCACTC AACTTCTCTC ACCATCTGCT 
GAACCCGCTT ACGTTTCAAC ATACCTCAGT CACAGTTTTC CTCAACACCG TCAGTATCTA 
TGTGCTGGTG CTTGTCTGCT GATGCAAGGC CACGCTGAAA ACATCAACAG CACAAATCTG 
GCACGAGTTC TGAGAGAAGT TTCTCCTGAA GAAGTCACTG CTAACATATA CACTTTGGTC 
GATGTTTTGC TTCACCACGT TCATGTAGAC CTTCAGCAAG GACAATCTCT AGAGGCAGTT 
CTAGACAAGG CAGGCGCAAA TCTTGCATTT TTCTTCTGGA CACATGAAAT GCTTCCTCTC 
GACATTTTTC TTTTGGCGCT CATTGACCGT GATGATGATC CTCACGCCTT GATAATTGCA 
ATGAGCCTAC TTAAAACGCC AGACCTTCTG CTGAGGATAA AAAACTACTG CCAAAACCGT 
GGGTCTCCTG AGCACTGGCT TGTCACACAG GTGTTCAAAC GCAATGAACT CCAGAAGGCC 
CTGGGTAACC ATCTTTCTTG GAAGGACAGG TATCCAACGT TCTTTGATGA TATTGCAGCG 
CGTTTGCTTC CAGTTATCCC TCTAGTGCTT TACAGGCTCA TCGAGAACAA TGCGATGGAG 
CAAGCTGATA ATCTTTTGCT TGCACACTCG CATTTTCTAG CGTATCATCC TCTCAGATTC 
ACGTTTGTCC GTGATATACT AGCCTATTTC TATGGCCATC TTCCTGGGAA ACTTGTTCTG 
CGCATGCTCA AAGTACTTGA TCTCAGCAAG ATTCCATTCT CTGAATCATT CCCGCAGTAC 
ATTAGCCCTA CCGGTGCACC TGTATGCCCA CCTCTAGATT ACTTTGCCTC TCTTCTGCTT 
AATCTCGTAA ACAATGTTAT ACCTCCACTT AGCAGCAGCA GCAACTGCAG CTCGAGGTCT 
GGCTCAATGG CAGACATCTT AAACAGCTCA GCAAGACCTC CTCATGGAAA AACTCCAGGA 
ACATCTCAGC CTGGACCAGC AAACGCCTCT GAGGGCCAGA AAGCATTCTA TCAGATCCAG 
GACCCAGGAA CTTACACGCA ACTGGTTCTT GAGACAGCCG TGATCGAGAT CCTCTCGCTT 
CCTGTCTCTG CTGCTCAGAT TGTCTCTTCG CTTGTCCAGA TAATTGTCAA CATACAATCG 
ACTCTGATTC AATCTGGAAA CGGGTTCCAC GGCGCTGCCA ACGGGGTGGG ACAAGGGTCG 
GTACTGCCAA CGTCTCCCTC TGGAGGGAGC ACAGACTCCA TGAGCGCTAG CCGGTCCACT 
TGTCTGATTC CTGGTATCAA CACAGCGAGC TTTGTCTCTA GAAGCGGTTA TACATGTCAG 
CAACTGTCGT GTCTGTTGAT TCAAGCTTGT GGACTTCTGT TGGCTCAGCT CCCTCCAGAT 
TTTCACGTAC AGCTTTACTT GGAGGCAGCA CGTGTGACAA GGGAAACATG GTGGCTTAAG 
GACGGGAAGA GATCACAAGG TGAACTAGAC TCAGCTGTTG GATACGCTTT GATGGATCCT 
ACATGGGCTG CTCAGGATAA CACCTCGACC GCCATAGGAA ATATTGTCGC CTTGCTTCAT 
GCTTTCTTCA GCAATCTCCC ACAAGAATGG CTTGATGGCA CGAATGCTAT CATCACGAAC 
CTCCGGCCTG TGACATCTGT GGCAATGCTC AGAGTTGTAT TCCGTATCAT GGGGCCACTG 
CTTCCGAGGC TTGCCAGTAC ACATACTCTC TTTAACAAGA CGCTAATGCT TCTCTTAAGC 
GCACTGGTCG ATGTTTTTGG AAAGACCGCA CAGACAACAG CCCCTGTTGA AGCATCCCAG 
ATTGCAGATC TTATTGATTT CCTACACCAT ATTATTCACT ACGAGGGACA AGGAGGAGCT 
GTTCAAACTA GCAGCAAGCC GAGACCGGAC ATCTTGGCGT TAATCGGAAG AGCAGCAGAG 
ACTCTCCGAC CAGATGTACA ACACCTTCTC GCTCACCTCA AAACCAACCC GAATTCTTCC 
ATATACGCAG CTGCTCATCA GCAGAATACT GCAAAGACTA ACACCTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT1G23230 ( NM_102172.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_173737.1 Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), partial mRNA.

Price & Availability↑

CloneID	OAb24312
Clone ID Related Accession (Same CDS sequence)	NM_102172.2
Accession Version	NM_102172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	mediator of RNA polymerase II transcription subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Apr 24, 2008 this sequence version replaced NM_102172.1. COMPLETENESS: incomplete on the 3' end.

ATGGATCAAT CGCAGCGAAC AGTCGCTGCA ACGCCATCGT CTTCTCGATC GTACCAATTC 
CACCCAGCTC GTGCTGCAAT TATCGATCTT TTCAATCTCT ACTTAGGGAG AGGAAGCCGT 
CAAAAGCCTG ATGAGTCCCT CCGAGATCCC CCGAACAAAT CACAGAAGCG TGTTCATGCT 
CCTAATAGAG ACTTACCACC AAGAAACGAG CAATTCCTTT TGGATTTTGA GCTGCTTCAG 
AGCCAATTCA ATGATCCAGA GCAATTGAGG ACGATTACTG AGTCAGTTTT GATATCTCTG 
GTGGTGCAAT GTAGCAATCA TGCGCCTCGT GCTGAGTTTC TACTCTTTGC TTTGCGTACA 
TTGTGTAGGA TTAGTTATAT CAATTGGGAT ACTTTTTTAC CATCCCTTCT CTCTTCCGTT 
TCCGCTGCTG AGGCGTCGTT AAGCCAAGGT GTTCAAGCAG CAGCGGCAAC GGCTGGTTCT 
TCTGCTACTT CTTCGCAGTC TGTAGTTCCT GTGTCTGTGA ATCCAACGTC ATTGCTGCCT 
TCAGCTCATG GTATTGGTTC TCCATCAGCT TCAGAGGTGA AATCTGTGGA GAATGGGCAG 
CAGATTGCAA GAGCTGGCCA GATTGTAAGA GAGAATGCTA TGAGAAATAG TCAACGGATT 
AGAGCTGCTG CTGTAAACAG TCTGCGTCAG CTCAGTTGCA AGATTATCTT GATTGGCGTT 
GAATCTAGTC TCAAACCCGT TACTCATGCC GAGATTTTCC AGTACATGAT GAATTGGCTT 
GTTAACTGGG ACAGGAGAGA TCTGGGAACT GAGGACTCTG TAGGAAAGTC GTGGAGATCT 
GAGAAGACTT TAGCTGAATG GTTGCGGAGT TGTTTGGATG TGATCTGGCT GTTGGTAGAA 
GAGGGTGAAT CTCGAATTCC GTTTTATGAG TTATTGCGCA GTGGTCTACA GTTTATAGAG 
AACATACCTG ATGATGAAGC CTTGTTCACT CTTATCATGG AGATACACCG GAGGCGAGAT 
GCGATGGCCA TGCATATGCT CATGTTGGAC CAACATCTTC ACTGTCCATC ATTTGGTACC 
CATCGTATAG TCTCCCAAAT AACTGCCAAT GTTCCACCCG AAGCTGTGCC ACACTTACGA 
CATTCACCAA TTACATATCC AAGTGTGCTT GGGGAACCTT TATATGGAGA GGATCTGGCG 
ATGTCTATTC CAAAAGGAAG CCTGGATTGG GAGAGAGCAG TGCGTTGCAT TAGACATGCT 
ATTCGCACTA CACCGTCACC GGACTGGTGG AAGCGTGTTC TTGTTGTGGC CCCTTGTTAT 
AGACCATCTA CACAAGCAGG GCCTATCCCT GGTGCTGTAT TCACTTCTGA CATGATTTGT 
GAGGCAATCA TTGACAGAAT TGTTGAGCTT CTTAAGCTGA CCAACTCAGG CAATGATTGC 
TTTGGTATTG ATTTGGTATC TGTTACTTTT CCCCCTCTCT ATGCAGATGC AAATTGCTGG 
CAAGAGTGGC TGGTTTTCTC GGATATATTC TTCTTTCTAA TCAAAAGTGG GTGTACTGAT 
TTTGTTGATT TCATTGACAA GTTGGTTTTA CGCCTTAACG GTGTTGATAA CCATATTCTT 
CGGACGAATC ATGTGACTTG GCTGCTTGCA CAAATAATAC GCGTGGAGCT TGTGATGACT 
GCTTTGAACT CAGATGCTAA AAAGGTGGAG ACTACTAGGA AGATCTTATC ATTTCACAGG 
GAAGACAGAA ATTCTGATCC CAACAATCCT CAGAGTGTGT TGCTTGACTT CGTAAGCAGT 
TGTCAGAATC TACGGATTTG GTCACTAAGC ACAACAACTA GGGCATACCT AAACAATGAA 
CAGCTGTTGA AGGGAAAACA GATCGATGAG TGGTGGAGAA GCAAAGGAGA GCGGATGATG 
GATTATATGA ATATGGATGA TAGGTCGATT GGCATGTTTT GGGTTGTTTC CTACACCATG 
GCACAACCAG CATGTGAAAC AGTTATAAAT TGGCTATCTT CAGCTGGCAT GGCTGAGTTA 
CCGGGATTAC AGCCAAACGA CCGAGTAATG ATGACGCAGG AAGTTACCCC GTTACCTATG 
TCATTGCTAT CTGGCTTTTC AATGAATTTG TGCTTGAAAT TGGCCCTTCA GATGGAAGAA 
GCTTTGTTTG TTAGCCAGGT TGTTCCTAGC ATTGCAATGG TTGAGACATA CACAAGATTG 
CTTCTGATTT CCCCTCATTC GATGTTCCGT TCACATTTCA GTCAATTGGC CCAGCGAAAT 
GCTTCTCTGC TAAGCAAGCC TGGAGTGACG TTACTTGTTC TTGAGATTCT GAACTATCGG 
TTGCTGCCAC TCTACAGATA CCAAGGAAAG AGTAAAACTT TAATGTATGA TGTTACTAAA 
ATAATATCTG CATTGAAAGG AAAACGTGGT GACCACCGTA TATTCAGACT AGCTGAAAAC 
TTATGCATGA ATCTCATTTT ATCACTCAGA GACTTTTTCT CTGTGAAACG GGAGGGAAAA 
GGGCCAACTG AATTCACTGA GACGTTAAAT CGCATAACCA TCATGACTCT TGCTATCACT 
ATCAAAACAC GTGGTATCGC AGACCCTGAT CACATGGTTT ATCTGCAAAC CATGTTGGAA 
CAAATACTCG CGACGAGTCA GCATACATGG TCAGAGAAGA CGATGCGGCA TTTCCCATCC 
CTTCTCCGTG AAACTTTGAA AGGACGGGTA GACAAGAGGG GCCTATCAAT TCAGGCATGG 
CAACAGGCTG AAACAACTGT GATAAACCAG TGCACTCAAC TTCTCTCACC ATCTGCTGAA 
CCCGCTTACG TTTCAACATA CCTCAGTCAC AGTTTTCCTC AACACCGTCA GTATCTATGT 
GCTGGTGCTT GTCTGCTGAT GCAAGGCCAC GCTGAAAACA TCAACAGCAC AAATCTGGCA 
CGAGTTCTGA GAGAAGTTTC TCCTGAAGAA GTCACTGCTA ACATATACAC TTTGGTCGAT 
GTTTTGCTTC ACCACGTTCA TGTAGACCTT CAGCAAGGAC AATCTCTAGA GGCAGTTCTA 
GACAAGGCAG GCGCAAATCT TGCATTTTTC TTCTGGACAC ATGAAATGCT TCCTCTCGAC 
ATTTTTCTTT TGGCGCTCAT TGACCGTGAT GATGATCCTC ACGCCTTGAT AATTGCAATG 
AGCCTACTTA AAACGCCAGA CCTTCTGCTG AGGATAAAAA ACTACTGCCA AAACCGTGGG 
TCTCCTGAGC ACTGGCTTGT CACACAGGTG TTCAAACGCA ATGAACTCCA GAAGGCCCTG 
GGTAACCATC TTTCTTGGAA GGACAGGTAT CCAACGTTCT TTGATGATAT TGCAGCGCGT 
TTGCTTCCAG TTATCCCTCT AGTGCTTTAC AGGCTCATCG AGAACAATGC GATGGAGCAA 
GCTGATAATC TTTTGCTTGC ACACTCGCAT TTTCTAGCGT ATCATCCTCT CAGATTCACG 
TTTGTCCGTG ATATACTAGC CTATTTCTAT GGCCATCTTC CTGGGAAACT TGTTCTGCGC 
ATGCTCAAAG TACTTGATCT CAGCAAGATT CCATTCTCTG AATCATTCCC GCAGTACATT 
AGCCCTACCG GTGCACCTGT ATGCCCACCT CTAGATTACT TTGCCTCTCT TCTGCTTAAT 
CTCGTAAACA ATGTTATACC TCCACTTAGC AGCAGCAGCA ACTGCAGCTC GAGGTCTGGC 
TCAATGGCAG ACATCTTAAA CAGCTCAGCA AGACCTCCTC ATGGAAAAAC TCCAGGAACA 
TCTCAGCCTG GACCAGCAAA CGCCTCTGAG GGCCAGAAAG CATTCTATCA GATCCAGGAC 
CCAGGAACTT ACACGCAACT GGTTCTTGAG ACAGCCGTGA TCGAGATCCT CTCGCTTCCT 
GTCTCTGCTG CTCAGATTGT CTCTTCGCTT GTCCAGATAA TTGTCAACAT ACAATCGACT 
CTGATTCAAT CTGGAAACGG GTTCCACGGC GCTGCCAACG GGGTGGGACA AGGGTCGGTA 
CTGCCAACGT CTCCCTCTGG AGGGAGCACA GACTCCATGA GCGCTAGCCG GTCCACTTGT 
CTGATTCCTG GTATCAACAC AGCGAGCTTT GTCTCTAGAA GCGGTTATAC ATGTCAGCAA 
CTGTCGTGTC TGTTGATTCA AGCTTGTGGA CTTCTGTTGG CTCAGCTCCC TCCAGATTTT 
CACGTACAGC TTTACTTGGA GGCAGCACGT GTGACAAGGG AAACATGGTG GCTTAAGGAC 
GGGAAGAGAT CACAAGGTGA ACTAGACTCA GCTGTTGGAT ACGCTTTGAT GGATCCTACA 
TGGGCTGCTC AGGATAACAC CTCGACCGCC ATAGGAAATA TTGTCGCCTT GCTTCATGCT 
TTCTTCAGCA ATCTCCCACA AGAATGGCTT GATGGCACGA ATGCTATCAT CACGAACCTC 
CGGCCTGTGA CATCTGTGGC AATGCTCAGA GTTGTATTCC GTATCATGGG GCCACTGCTT 
CCGAGGCTTG CCAGTACACA TACTCTCTTT AACAAGACGC TAATGCTTCT CTTAAGCGCA 
CTGGTCGATG TTTTTGGAAA GACCGCACAG ACAACAGCCC CTGTTGAAGC ATCCCAGATT 
GCAGATCTTA TTGATTTCCT ACACCATATT ATTCACTACG AGGGACAAGG AGGAGCTGTT 
CAAACTAGCA GCAAGCCGAG ACCGGACATC TTGGCGTTAA TCGGAAGAGC AGCAGAGACT 
CTCCGACCAG ATGTACAACA CCTTCTCGCT CACCTCAAAA CCAACCCGAA TTCTTCCATA 
TACGCAGCTG CTCATCAGCA GAATACTGCA AAGACTAACA CCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_173737.1
CDS	42..4889
Translation	MDQSQRTVAATPSSSRSYQFHPARAAIIDLFNLYLGRGSRQKPDESLRDPPNKSQKRVHAPNRDLPPRNEQFLLDFELLQSQFNDPEQLRTITESVLISLVVQCSNHAPRAEFLLFALRTLCRISYINWDTFLPSLLSSVSAAEASLSQGVQAAAATAGSSATSSQSVVPVSVNPTSLLPSAHGIGSPSASEVKSVENGQQIARAGQIVRENAMRNSQRIRAAAVNSLRQLSCKIILIGVESSLKPVTHAEIFQYMMNWLVNWDRRDLGTEDSVGKSWRSEKTLAEWLRSCLDVIWLLVEEGESRIPFYELLRSGLQFIENIPDDEALFTLIMEIHRRRDAMAMHMLMLDQHLHCPSFGTHRIVSQITANVPPEAVPHLRHSPITYPSVLGEPLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSPDWWKRVLVVAPCYRPSTQAGPIPGAVFTSDMICEAIIDRIVELLKLTNSGNDCFGIDLVSVTFPPLYADANCWQEWLVFSDIFFFLIKSGCTDFVDFIDKLVLRLNGVDNHILRTNHVTWLLAQIIRVELVMTALNSDAKKVETTRKILSFHREDRNSDPNNPQSVLLDFVSSCQNLRIWSLSTTTRAYLNNEQLLKGKQIDEWWRSKGERMMDYMNMDDRSIGMFWVVSYTMAQPACETVINWLSSAGMAELPGLQPNDRVMMTQEVTPLPMSLLSGFSMNLCLKLALQMEEALFVSQVVPSIAMVETYTRLLLISPHSMFRSHFSQLAQRNASLLSKPGVTLLVLEILNYRLLPLYRYQGKSKTLMYDVTKIISALKGKRGDHRIFRLAENLCMNLILSLRDFFSVKREGKGPTEFTETLNRITIMTLAITIKTRGIADPDHMVYLQTMLEQILATSQHTWSEKTMRHFPSLLRETLKGRVDKRGLSIQAWQQAETTVINQCTQLLSPSAEPAYVSTYLSHSFPQHRQYLCAGACLLMQGHAENINSTNLARVLREVSPEEVTANIYTLVDVLLHHVHVDLQQGQSLEAVLDKAGANLAFFFWTHEMLPLDIFLLALIDRDDDPHALIIAMSLLKTPDLLLRIKNYCQNRGSPEHWLVTQVFKRNELQKALGNHLSWKDRYPTFFDDIAARLLPVIPLVLYRLIENNAMEQADNLLLAHSHFLAYHPLRFTFVRDILAYFYGHLPGKLVLRMLKVLDLSKIPFSESFPQYISPTGAPVCPPLDYFASLLLNLVNNVIPPLSSSSNCSSRSGSMADILNSSARPPHGKTPGTSQPGPANASEGQKAFYQIQDPGTYTQLVLETAVIEILSLPVSAAQIVSSLVQIIVNIQSTLIQSGNGFHGAANGVGQGSVLPTSPSGGSTDSMSASRSTCLIPGINTASFVSRSGYTCQQLSCLLIQACGLLLAQLPPDFHVQLYLEAARVTRETWWLKDGKRSQGELDSAVGYALMDPTWAAQDNTSTAIGNIVALLHAFFSNLPQEWLDGTNAIITNLRPVTSVAMLRVVFRIMGPLLPRLASTHTLFNKTLMLLLSALVDVFGKTAQTTAPVEASQIADLIDFLHHIIHYEGQGGAVQTSSKPRPDILALIGRAAETLRPDVQHLLAHLKTNPNSSIYAAAHQQNTAKTNTS

Target ORF information:

RefSeq Version	NM_102172.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_102172.2

ORF Insert Sequence:

ATGGATCAAT CGCAGCGAAC AGTCGCTGCA ACGCCATCGT CTTCTCGATC GTACCAATTC 
CACCCAGCTC GTGCTGCAAT TATCGATCTT TTCAATCTCT ACTTAGGGAG AGGAAGCCGT 
CAAAAGCCTG ATGAGTCCCT CCGAGATCCC CCGAACAAAT CACAGAAGCG TGTTCATGCT 
CCTAATAGAG ACTTACCACC AAGAAACGAG CAATTCCTTT TGGATTTTGA GCTGCTTCAG 
AGCCAATTCA ATGATCCAGA GCAATTGAGG ACGATTACTG AGTCAGTTTT GATATCTCTG 
GTGGTGCAAT GTAGCAATCA TGCGCCTCGT GCTGAGTTTC TACTCTTTGC TTTGCGTACA 
TTGTGTAGGA TTAGTTATAT CAATTGGGAT ACTTTTTTAC CATCCCTTCT CTCTTCCGTT 
TCCGCTGCTG AGGCGTCGTT AAGCCAAGGT GTTCAAGCAG CAGCGGCAAC GGCTGGTTCT 
TCTGCTACTT CTTCGCAGTC TGTAGTTCCT GTGTCTGTGA ATCCAACGTC ATTGCTGCCT 
TCAGCTCATG GTATTGGTTC TCCATCAGCT TCAGAGGTGA AATCTGTGGA GAATGGGCAG 
CAGATTGCAA GAGCTGGCCA GATTGTAAGA GAGAATGCTA TGAGAAATAG TCAACGGATT 
AGAGCTGCTG CTGTAAACAG TCTGCGTCAG CTCAGTTGCA AGATTATCTT GATTGGCGTT 
GAATCTAGTC TCAAACCCGT TACTCATGCC GAGATTTTCC AGTACATGAT GAATTGGCTT 
GTTAACTGGG ACAGGAGAGA TCTGGGAACT GAGGACTCTG TAGGAAAGTC GTGGAGATCT 
GAGAAGACTT TAGCTGAATG GTTGCGGAGT TGTTTGGATG TGATCTGGCT GTTGGTAGAA 
GAGGGTGAAT CTCGAATTCC GTTTTATGAG TTATTGCGCA GTGGTCTACA GTTTATAGAG 
AACATACCTG ATGATGAAGC CTTGTTCACT CTTATCATGG AGATACACCG GAGGCGAGAT 
GCGATGGCCA TGCATATGCT CATGTTGGAC CAACATCTTC ACTGTCCATC ATTTGGTACC 
CATCGTATAG TCTCCCAAAT AACTGCCAAT GTTCCACCCG AAGCTGTGCC ACACTTACGA 
CATTCACCAA TTACATATCC AAGTGTGCTT GGGGAACCTT TATATGGAGA GGATCTGGCG 
ATGTCTATTC CAAAAGGAAG CCTGGATTGG GAGAGAGCAG TGCGTTGCAT TAGACATGCT 
ATTCGCACTA CACCGTCACC GGACTGGTGG AAGCGTGTTC TTGTTGTGGC CCCTTGTTAT 
AGACCATCTA CACAAGCAGG GCCTATCCCT GGTGCTGTAT TCACTTCTGA CATGATTTGT 
GAGGCAATCA TTGACAGAAT TGTTGAGCTT CTTAAGCTGA CCAACTCAGG CAATGATTGC 
TTTGGTATTG ATTTGGTATC TGTTACTTTT CCCCCTCTCT ATGCAGATGC AAATTGCTGG 
CAAGAGTGGC TGGTTTTCTC GGATATATTC TTCTTTCTAA TCAAAAGTGG GTGTACTGAT 
TTTGTTGATT TCATTGACAA GTTGGTTTTA CGCCTTAACG GTGTTGATAA CCATATTCTT 
CGGACGAATC ATGTGACTTG GCTGCTTGCA CAAATAATAC GCGTGGAGCT TGTGATGACT 
GCTTTGAACT CAGATGCTAA AAAGGTGGAG ACTACTAGGA AGATCTTATC ATTTCACAGG 
GAAGACAGAA ATTCTGATCC CAACAATCCT CAGAGTGTGT TGCTTGACTT CGTAAGCAGT 
TGTCAGAATC TACGGATTTG GTCACTAAGC ACAACAACTA GGGCATACCT AAACAATGAA 
CAGCTGTTGA AGGGAAAACA GATCGATGAG TGGTGGAGAA GCAAAGGAGA GCGGATGATG 
GATTATATGA ATATGGATGA TAGGTCGATT GGCATGTTTT GGGTTGTTTC CTACACCATG 
GCACAACCAG CATGTGAAAC AGTTATAAAT TGGCTATCTT CAGCTGGCAT GGCTGAGTTA 
CCGGGATTAC AGCCAAACGA CCGAGTAATG ATGACGCAGG AAGTTACCCC GTTACCTATG 
TCATTGCTAT CTGGCTTTTC AATGAATTTG TGCTTGAAAT TGGCCCTTCA GATGGAAGAA 
GCTTTGTTTG TTAGCCAGGT TGTTCCTAGC ATTGCAATGG TTGAGACATA CACAAGATTG 
CTTCTGATTT CCCCTCATTC GATGTTCCGT TCACATTTCA GTCAATTGGC CCAGCGAAAT 
GCTTCTCTGC TAAGCAAGCC TGGAGTGACG TTACTTGTTC TTGAGATTCT GAACTATCGG 
TTGCTGCCAC TCTACAGATA CCAAGGAAAG AGTAAAACTT TAATGTATGA TGTTACTAAA 
ATAATATCTG CATTGAAAGG AAAACGTGGT GACCACCGTA TATTCAGACT AGCTGAAAAC 
TTATGCATGA ATCTCATTTT ATCACTCAGA GACTTTTTCT CTGTGAAACG GGAGGGAAAA 
GGGCCAACTG AATTCACTGA GACGTTAAAT CGCATAACCA TCATGACTCT TGCTATCACT 
ATCAAAACAC GTGGTATCGC AGACCCTGAT CACATGGTTT ATCTGCAAAC CATGTTGGAA 
CAAATACTCG CGACGAGTCA GCATACATGG TCAGAGAAGA CGATGCGGCA TTTCCCATCC 
CTTCTCCGTG AAACTTTGAA AGGACGGGTA GACAAGAGGG GCCTATCAAT TCAGGCATGG 
CAACAGGCTG AAACAACTGT GATAAACCAG TGCACTCAAC TTCTCTCACC ATCTGCTGAA 
CCCGCTTACG TTTCAACATA CCTCAGTCAC AGTTTTCCTC AACACCGTCA GTATCTATGT 
GCTGGTGCTT GTCTGCTGAT GCAAGGCCAC GCTGAAAACA TCAACAGCAC AAATCTGGCA 
CGAGTTCTGA GAGAAGTTTC TCCTGAAGAA GTCACTGCTA ACATATACAC TTTGGTCGAT 
GTTTTGCTTC ACCACGTTCA TGTAGACCTT CAGCAAGGAC AATCTCTAGA GGCAGTTCTA 
GACAAGGCAG GCGCAAATCT TGCATTTTTC TTCTGGACAC ATGAAATGCT TCCTCTCGAC 
ATTTTTCTTT TGGCGCTCAT TGACCGTGAT GATGATCCTC ACGCCTTGAT AATTGCAATG 
AGCCTACTTA AAACGCCAGA CCTTCTGCTG AGGATAAAAA ACTACTGCCA AAACCGTGGG 
TCTCCTGAGC ACTGGCTTGT CACACAGGTG TTCAAACGCA ATGAACTCCA GAAGGCCCTG 
GGTAACCATC TTTCTTGGAA GGACAGGTAT CCAACGTTCT TTGATGATAT TGCAGCGCGT 
TTGCTTCCAG TTATCCCTCT AGTGCTTTAC AGGCTCATCG AGAACAATGC GATGGAGCAA 
GCTGATAATC TTTTGCTTGC ACACTCGCAT TTTCTAGCGT ATCATCCTCT CAGATTCACG 
TTTGTCCGTG ATATACTAGC CTATTTCTAT GGCCATCTTC CTGGGAAACT TGTTCTGCGC 
ATGCTCAAAG TACTTGATCT CAGCAAGATT CCATTCTCTG AATCATTCCC GCAGTACATT 
AGCCCTACCG GTGCACCTGT ATGCCCACCT CTAGATTACT TTGCCTCTCT TCTGCTTAAT 
CTCGTAAACA ATGTTATACC TCCACTTAGC AGCAGCAGCA ACTGCAGCTC GAGGTCTGGC 
TCAATGGCAG ACATCTTAAA CAGCTCAGCA AGACCTCCTC ATGGAAAAAC TCCAGGAACA 
TCTCAGCCTG GACCAGCAAA CGCCTCTGAG GGCCAGAAAG CATTCTATCA GATCCAGGAC 
CCAGGAACTT ACACGCAACT GGTTCTTGAG ACAGCCGTGA TCGAGATCCT CTCGCTTCCT 
GTCTCTGCTG CTCAGATTGT CTCTTCGCTT GTCCAGATAA TTGTCAACAT ACAATCGACT 
CTGATTCAAT CTGGAAACGG GTTCCACGGC GCTGCCAACG GGGTGGGACA AGGGTCGGTA 
CTGCCAACGT CTCCCTCTGG AGGGAGCACA GACTCCATGA GCGCTAGCCG GTCCACTTGT 
CTGATTCCTG GTATCAACAC AGCGAGCTTT GTCTCTAGAA GCGGTTATAC ATGTCAGCAA 
CTGTCGTGTC TGTTGATTCA AGCTTGTGGA CTTCTGTTGG CTCAGCTCCC TCCAGATTTT 
CACGTACAGC TTTACTTGGA GGCAGCACGT GTGACAAGGG AAACATGGTG GCTTAAGGAC 
GGGAAGAGAT CACAAGGTGA ACTAGACTCA GCTGTTGGAT ACGCTTTGAT GGATCCTACA 
TGGGCTGCTC AGGATAACAC CTCGACCGCC ATAGGAAATA TTGTCGCCTT GCTTCATGCT 
TTCTTCAGCA ATCTCCCACA AGAATGGCTT GATGGCACGA ATGCTATCAT CACGAACCTC 
CGGCCTGTGA CATCTGTGGC AATGCTCAGA GTTGTATTCC GTATCATGGG GCCACTGCTT 
CCGAGGCTTG CCAGTACACA TACTCTCTTT AACAAGACGC TAATGCTTCT CTTAAGCGCA 
CTGGTCGATG TTTTTGGAAA GACCGCACAG ACAACAGCCC CTGTTGAAGC ATCCCAGATT 
GCAGATCTTA TTGATTTCCT ACACCATATT ATTCACTACG AGGGACAAGG AGGAGCTGTT 
CAAACTAGCA GCAAGCCGAG ACCGGACATC TTGGCGTTAA TCGGAAGAGC AGCAGAGACT 
CTCCGACCAG ATGTACAACA CCTTCTCGCT CACCTCAAAA CCAACCCGAA TTCTTCCATA 
TACGCAGCTG CTCATCAGCA GAATACTGCA AAGACTAACA CCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT1G23230 ( NM_001198142.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185071.1 Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), mRNA.

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CloneID	OAb31143
Clone ID Related Accession (Same CDS sequence)	NM_001198142.2
Accession Version	NM_001198142.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	mediator of RNA polymerase II transcription subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_001198142.1.

ATGGATCAAT CGCAGCGAAC AGTCGCTGCA ACGCCATCGT CTTCTCGATC GTACCAATTC 
CACCCAGCTC GTGCTGCAAT TATCGATCTT TTCAATCTCT ACTTAGGGAG AGGAAGCCGT 
CAAAAGCCTG ATGAGTCCCT CCGAGATCCC CCGAACAAAT CACAGAAGCG TGTTCATGCT 
CCTAATAGAG ACTTACCACC AAGAAACGAG CAATTCCTTT TGGATTTTGA GCTGCTTCAG 
AGCCAATTCA ATGATCCAGA GCAATTGAGG ACGATTACTG AGTCAGTTTT GATATCTCTG 
GTGGTGCAAT GTAGCAATCA TGCGCCTCGT GCTGAGTTTC TACTCTTTGC TTTGCGTACA 
TTGTGTAGGA TTAGTTATAT CAATTGGGAT ACTTTTTTAC CATCCCTTCT CTCTTCCGTT 
TCCGCTGCTG AGGCGTCGTT AAGCCAAGGT GTTCAAGCAG CAGCGGCAAC GGCTGGTTCT 
TCTGCTACTT CTTCGCAGTC TGTAGTTCCT GTGTCTGTGA ATCCAACGTC ATTGCTGCCT 
TCAGCTCATG GTATTGGTTC TCCATCAGCT TCAGAGGTGA AATCTGTGGA GAATGGGCAG 
CAGATTGCAA GAGCTGGCCA GATTGTAAGA GAGAATGCTA TGAGAAATAG TCAACGGATT 
AGAGCTGCTG CTGTAAACAG TCTGCGTCAG CTCAGTTGCA AGATTATCTT GATTGGCGTT 
GAATCTAGTC TCAAACCCGT TACTCATGCC GAGATTTTCC AGTACATGAT GAATTGGCTT 
GTTAACTGGG ACAGGAGAGA TCTGGGAACT GAGGACTCTG TAGGAAAGTC GTGGAGATCT 
GAGAAGACTT TAGCTGAATG GTTGCGGAGT TGTTTGGATG TGATCTGGCT GTTGGTAGAA 
GAGGGTGAAT CTCGAATTCC GTTTTATGAG TTATTGCGCA GTGGTCTACA GTTTATAGAG 
AACATACCTG ATGATGAAGC CTTGTTCACT CTTATCATGG AGATACACCG GAGGCGAGAT 
GCGATGGCCA TGCATATGCT CATGTTGGAC CAACATCTTC ACTGTCCATC ATTTGGTACC 
CATCGTATAG TCTCCCAAAT AACTGCCAAT GTTCCACCCG AAGCTGTGCC ACACTTACGA 
CATTCACCAA TTACATATCC AAGTGTGCTT GGGGAACCTT TATATGGAGA GGATCTGGCG 
ATGTCTATTC CAAAAGGAAG CCTGGATTGG GAGAGAGCAG TGCGTTGCAT TAGACATGCT 
ATTCGCACTA CACCGTCACC GGACTGGTGG AAGCGTGTTC TTGTTGTGGC CCCTTGTTAT 
AGACCATCTA CACAAGCAGG GCCTATCCCT GGTGCTGTAT TCACTTCTGA CATGATTTGT 
GAGGCAATCA TTGACAGAAT TGTTGAGCTT CTTAAGCTGA CCAACTCAGA TGCAAATTGC 
TGGCAAGAGT GGCTGGTTTT CTCGGATATA TTCTTCTTTC TAATCAAAAG TGGGTGTACT 
GATTTTGTTG ATTTCATTGA CAAGTTGGTT TTACGCCTTA ACGGTGTTGA TAACCATATT 
CTTCGGACGA ATCATGTGAC TTGGCTGCTT GCACAAATAA TACGCGTGGA GCTTGTGATG 
ACTGCTTTGA ACTCAGATGC TAAAAAGGTG GAGACTACTA GGAAGATCTT ATCATTTCAC 
AGGGAAGACA GAAATTCTGA TCCCAACAAT CCTCAGAGTG TGTTGCTTGA CTTCGTAAGC 
AGTTGTCAGA ATCTACGGAT TTGGTCACTA AGCACAACAA CTAGGGCATA CCTAAACAAT 
GAACAGCTGT TGAAGGGAAA ACAGATCGAT GAGTGGTGGA GAAGCAAAGG AGAGCGGATG 
ATGGATTATA TGAATATGGA TGATAGGTCG ATTGGCATGT TTTGGGTTGT TTCCTACACC 
ATGGCACAAC CAGCATGTGA AACAGTTATA AATTGGCTAT CTTCAGCTGG CATGGCTGAG 
TTACCGGGAT TACAGCCAAA CGACCGAGTA ATGATGACGC AGGAAGTTAC CCCGTTACCT 
ATGTCATTGC TATCTGGCTT TTCAATGAAT TTGTGCTTGA AATTGGCCCT TCAGATGGAA 
GAAGCTTTGT TTGTTAGCCA GGTTGTTCCT AGCATTGCAA TGGTTGAGAC ATACACAAGA 
TTGCTTCTGA TTTCCCCTCA TTCGATGTTC CGTTCACATT TCAGTCGAAA TGCTTCTCTG 
CTAAGCAAGC CTGGAGTGAC GTTACTTGTT CTTGAGATTC TGAACTATCG GTTGCTGCCA 
CTCTACAGAT ACCAAGGAAA GAGTAAAACT TTAATGTATG ATGTTACTAA AATAATATCT 
GCATTGAAAG GAAAACGTGG TGACCACCGT ATATTCAGAC TAGCTGAAAA CTTATGCATG 
AATCTCATTT TATCACTCAG AGACTTTTTC TCTGTGAAAC GGGAGGGAAA AGGGCCAACT 
GAATTCACTG AGACGTTAAA TCGCATAACC ATCATGACTC TTGCTATCAC TATCAAAACA 
CGTGGTATCG CAGACCCTGA TCACATGGTT TATCTGCAAA CCATGTTGGA ACAAATACTC 
GCGACGAGTC AGCATACATG GTCAGAGAAG ACGATGCGGC ATTTCCCATC CCTTCTCCGT 
GAAACTTTGA AAGGACGGGT AGACAAGAGG GGCCTATCAA TTCAGGCATG GCAACAGGCT 
GAAACAACTG TGATAAACCA GTGCACTCAA CTTCTCTCAC CATCTGCTGA ACCCGCTTAC 
GTTTCAACAT ACCTCAGTCA CAGTTTTCCT CAACACCGTC AGTATCTATG TGCTGGTGCT 
TGTCTGCTGA TGCAAGGCCA CGCTGAAAAC ATCAACAGCA CAAATCTGGC ACGAGTTCTG 
AGAGAAGTTT CTCCTGAAGA AGTCACTGCT AACATATACA CTTTGGTCGA TGTTTTGCTT 
CACCACGTTC ATGTAGACCT TCAGCAAGGA CAATCTCTAG AGGCAGTTCT AGACAAGGCA 
GGCGCAAATC TTGCATTTTT CTTCTGGACA CATGAAATGC TTCCTCTCGA CATTTTTCTT 
TTGGCGCTCA TTGACCGTGA TGATGATCCT CACGCCTTGA TAATTGCAAT GAGCCTACTT 
AAAACGCCAG ACCTTCTGCT GAGGATAAAA AACTACTGCC AAAACCGTGG GTCTCCTGAG 
CACTGGCTTG TCACACAGGT GTTCAAACGC AATGAACTCC AGAAGGCCCT GGGTAACCAT 
CTTTCTTGGA AGGACAGGTA TCCAACGTTC TTTGATGATA TTGCAGCGCG TTTGCTTCCA 
GTTATCCCTC TAGTGCTTTA CAGGCTCATC GAGAACAATG CGATGGAGCA AGCTGATAAT 
CTTTTGCTTG CACACTCGCA TTTTCTAGCG TATCATCCTC TCAGATTCAC GTTTGTCCGT 
GATATACTAG CCTATTTCTA TGGCCATCTT CCTGGGAAAC TTGTTCTGCG CATGCTCAAA 
GTACTTGATC TCAGCAAGAT TCCATTCTCT GAATCATTCC CGCAGTACAT TAGCCCTACC 
GGTGCACCTG TATGCCCACC TCTAGATTAC TTTGCCTCTC TTCTGCTTAA TCTCGTAAAC 
AATGTTATAC CTCCACTTAG CAGCAGCAGC AACTGCAGCT CGAGGTCTGG CTCAATGGCA 
GACATCTTAA ACAGCTCAGC AAGACCTCCT CATGGAAAAA CTCCAGGAAC ATCTCAGCCT 
GGACCAGCAA ACGCCTCTGA GGGCCAGAAA GCATTCTATC AGATCCAGGA CCCAGGAACT 
TACACGCAAC TGGTTCTTGA GACAGCCGTG ATCGAGATCC TCTCGCTTCC TGTCTCTGCT 
GCTCAGATTG TCTCTTCGCT TGTCCAGATA ATTGTCAACA TACAATCGAC TCTGATTCAA 
TCTGGAAACG GGTTCCACGG CGCTGCCAAC GGGGTGGGAC AAGGGTCGGT ACTGCCAACG 
TCTCCCTCTG GAGGGAGCAC AGACTCCATG AGCGCTAGCC GGTCCACTTG TCTGATTCCT 
GGTATCAACA CAGCGAGCTT TGTCTCTAGA AGCGGTTATA CATGTCAGCA ACTGTCGTGT 
CTGTTGATTC AAGCTTGTGG ACTTCTGTTG GCTCAGCTCC CTCCAGATTT TCACGTACAG 
CTTTACTTGG AGGCAGCACG TGTGACAAGG GAAACATGGT GGCTTAAGGA CGGGAAGAGA 
TCACAAGGTG AACTAGACTC AGCTGTTGGA TACGCTTTGA TGGATCCTAC ATGGGCTGCT 
CAGGATAACA CCTCGACCGC CATAGGAAAT ATTGTCGCCT TGCTTCATGC TTTCTTCAGC 
AATCTCCCAC AAGAATGGCT TGATGGCACG AATGCTATCA TCACGAACCT CCGGCCTGTG 
ACATCTGTGG CAATGCTCAG AGTTGTATTC CGTATCATGG GGCCACTGCT TCCGAGGCTT 
GCCAGTACAC ATACTCTCTT TAACAAGACG CTAATGCTTC TCTTAAGCGC ACTGGTCGAT 
GTTTTTGGAA AGACCGCACA GACAACAGCC CCTGTTGAAG CATCCCAGAT TGCAGATCTT 
ATTGATTTCC TACACCATAT TATTCACTAC GAGGGACAAG GAGGAGCTGT TCAAACTAGC 
AGCAAGCCGA GACCGGACAT CTTGGCGTTA ATCGGAAGAG CAGCAGAGAC TCTCCGACCA 
GATGTACAAC ACCTTCTCGC TCACCTCAAA ACCAACCCGA ATTCTTCCAT ATACGCAGCT 
GCTCATCAGC AGAATACTGC AAAGACTAAC ACCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185071.1
CDS	64..4842
Translation	MDQSQRTVAATPSSSRSYQFHPARAAIIDLFNLYLGRGSRQKPDESLRDPPNKSQKRVHAPNRDLPPRNEQFLLDFELLQSQFNDPEQLRTITESVLISLVVQCSNHAPRAEFLLFALRTLCRISYINWDTFLPSLLSSVSAAEASLSQGVQAAAATAGSSATSSQSVVPVSVNPTSLLPSAHGIGSPSASEVKSVENGQQIARAGQIVRENAMRNSQRIRAAAVNSLRQLSCKIILIGVESSLKPVTHAEIFQYMMNWLVNWDRRDLGTEDSVGKSWRSEKTLAEWLRSCLDVIWLLVEEGESRIPFYELLRSGLQFIENIPDDEALFTLIMEIHRRRDAMAMHMLMLDQHLHCPSFGTHRIVSQITANVPPEAVPHLRHSPITYPSVLGEPLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSPDWWKRVLVVAPCYRPSTQAGPIPGAVFTSDMICEAIIDRIVELLKLTNSDANCWQEWLVFSDIFFFLIKSGCTDFVDFIDKLVLRLNGVDNHILRTNHVTWLLAQIIRVELVMTALNSDAKKVETTRKILSFHREDRNSDPNNPQSVLLDFVSSCQNLRIWSLSTTTRAYLNNEQLLKGKQIDEWWRSKGERMMDYMNMDDRSIGMFWVVSYTMAQPACETVINWLSSAGMAELPGLQPNDRVMMTQEVTPLPMSLLSGFSMNLCLKLALQMEEALFVSQVVPSIAMVETYTRLLLISPHSMFRSHFSRNASLLSKPGVTLLVLEILNYRLLPLYRYQGKSKTLMYDVTKIISALKGKRGDHRIFRLAENLCMNLILSLRDFFSVKREGKGPTEFTETLNRITIMTLAITIKTRGIADPDHMVYLQTMLEQILATSQHTWSEKTMRHFPSLLRETLKGRVDKRGLSIQAWQQAETTVINQCTQLLSPSAEPAYVSTYLSHSFPQHRQYLCAGACLLMQGHAENINSTNLARVLREVSPEEVTANIYTLVDVLLHHVHVDLQQGQSLEAVLDKAGANLAFFFWTHEMLPLDIFLLALIDRDDDPHALIIAMSLLKTPDLLLRIKNYCQNRGSPEHWLVTQVFKRNELQKALGNHLSWKDRYPTFFDDIAARLLPVIPLVLYRLIENNAMEQADNLLLAHSHFLAYHPLRFTFVRDILAYFYGHLPGKLVLRMLKVLDLSKIPFSESFPQYISPTGAPVCPPLDYFASLLLNLVNNVIPPLSSSSNCSSRSGSMADILNSSARPPHGKTPGTSQPGPANASEGQKAFYQIQDPGTYTQLVLETAVIEILSLPVSAAQIVSSLVQIIVNIQSTLIQSGNGFHGAANGVGQGSVLPTSPSGGSTDSMSASRSTCLIPGINTASFVSRSGYTCQQLSCLLIQACGLLLAQLPPDFHVQLYLEAARVTRETWWLKDGKRSQGELDSAVGYALMDPTWAAQDNTSTAIGNIVALLHAFFSNLPQEWLDGTNAIITNLRPVTSVAMLRVVFRIMGPLLPRLASTHTLFNKTLMLLLSALVDVFGKTAQTTAPVEASQIADLIDFLHHIIHYEGQGGAVQTSSKPRPDILALIGRAAETLRPDVQHLLAHLKTNPNSSIYAAAHQQNTAKTNTS

Target ORF information:

RefSeq Version	NM_001198142.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana mediator of RNA polymerase II transcription subunit (AT1G23230), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198142.2

ORF Insert Sequence:

ATGGATCAAT CGCAGCGAAC AGTCGCTGCA ACGCCATCGT CTTCTCGATC GTACCAATTC 
CACCCAGCTC GTGCTGCAAT TATCGATCTT TTCAATCTCT ACTTAGGGAG AGGAAGCCGT 
CAAAAGCCTG ATGAGTCCCT CCGAGATCCC CCGAACAAAT CACAGAAGCG TGTTCATGCT 
CCTAATAGAG ACTTACCACC AAGAAACGAG CAATTCCTTT TGGATTTTGA GCTGCTTCAG 
AGCCAATTCA ATGATCCAGA GCAATTGAGG ACGATTACTG AGTCAGTTTT GATATCTCTG 
GTGGTGCAAT GTAGCAATCA TGCGCCTCGT GCTGAGTTTC TACTCTTTGC TTTGCGTACA 
TTGTGTAGGA TTAGTTATAT CAATTGGGAT ACTTTTTTAC CATCCCTTCT CTCTTCCGTT 
TCCGCTGCTG AGGCGTCGTT AAGCCAAGGT GTTCAAGCAG CAGCGGCAAC GGCTGGTTCT 
TCTGCTACTT CTTCGCAGTC TGTAGTTCCT GTGTCTGTGA ATCCAACGTC ATTGCTGCCT 
TCAGCTCATG GTATTGGTTC TCCATCAGCT TCAGAGGTGA AATCTGTGGA GAATGGGCAG 
CAGATTGCAA GAGCTGGCCA GATTGTAAGA GAGAATGCTA TGAGAAATAG TCAACGGATT 
AGAGCTGCTG CTGTAAACAG TCTGCGTCAG CTCAGTTGCA AGATTATCTT GATTGGCGTT 
GAATCTAGTC TCAAACCCGT TACTCATGCC GAGATTTTCC AGTACATGAT GAATTGGCTT 
GTTAACTGGG ACAGGAGAGA TCTGGGAACT GAGGACTCTG TAGGAAAGTC GTGGAGATCT 
GAGAAGACTT TAGCTGAATG GTTGCGGAGT TGTTTGGATG TGATCTGGCT GTTGGTAGAA 
GAGGGTGAAT CTCGAATTCC GTTTTATGAG TTATTGCGCA GTGGTCTACA GTTTATAGAG 
AACATACCTG ATGATGAAGC CTTGTTCACT CTTATCATGG AGATACACCG GAGGCGAGAT 
GCGATGGCCA TGCATATGCT CATGTTGGAC CAACATCTTC ACTGTCCATC ATTTGGTACC 
CATCGTATAG TCTCCCAAAT AACTGCCAAT GTTCCACCCG AAGCTGTGCC ACACTTACGA 
CATTCACCAA TTACATATCC AAGTGTGCTT GGGGAACCTT TATATGGAGA GGATCTGGCG 
ATGTCTATTC CAAAAGGAAG CCTGGATTGG GAGAGAGCAG TGCGTTGCAT TAGACATGCT 
ATTCGCACTA CACCGTCACC GGACTGGTGG AAGCGTGTTC TTGTTGTGGC CCCTTGTTAT 
AGACCATCTA CACAAGCAGG GCCTATCCCT GGTGCTGTAT TCACTTCTGA CATGATTTGT 
GAGGCAATCA TTGACAGAAT TGTTGAGCTT CTTAAGCTGA CCAACTCAGA TGCAAATTGC 
TGGCAAGAGT GGCTGGTTTT CTCGGATATA TTCTTCTTTC TAATCAAAAG TGGGTGTACT 
GATTTTGTTG ATTTCATTGA CAAGTTGGTT TTACGCCTTA ACGGTGTTGA TAACCATATT 
CTTCGGACGA ATCATGTGAC TTGGCTGCTT GCACAAATAA TACGCGTGGA GCTTGTGATG 
ACTGCTTTGA ACTCAGATGC TAAAAAGGTG GAGACTACTA GGAAGATCTT ATCATTTCAC 
AGGGAAGACA GAAATTCTGA TCCCAACAAT CCTCAGAGTG TGTTGCTTGA CTTCGTAAGC 
AGTTGTCAGA ATCTACGGAT TTGGTCACTA AGCACAACAA CTAGGGCATA CCTAAACAAT 
GAACAGCTGT TGAAGGGAAA ACAGATCGAT GAGTGGTGGA GAAGCAAAGG AGAGCGGATG 
ATGGATTATA TGAATATGGA TGATAGGTCG ATTGGCATGT TTTGGGTTGT TTCCTACACC 
ATGGCACAAC CAGCATGTGA AACAGTTATA AATTGGCTAT CTTCAGCTGG CATGGCTGAG 
TTACCGGGAT TACAGCCAAA CGACCGAGTA ATGATGACGC AGGAAGTTAC CCCGTTACCT 
ATGTCATTGC TATCTGGCTT TTCAATGAAT TTGTGCTTGA AATTGGCCCT TCAGATGGAA 
GAAGCTTTGT TTGTTAGCCA GGTTGTTCCT AGCATTGCAA TGGTTGAGAC ATACACAAGA 
TTGCTTCTGA TTTCCCCTCA TTCGATGTTC CGTTCACATT TCAGTCGAAA TGCTTCTCTG 
CTAAGCAAGC CTGGAGTGAC GTTACTTGTT CTTGAGATTC TGAACTATCG GTTGCTGCCA 
CTCTACAGAT ACCAAGGAAA GAGTAAAACT TTAATGTATG ATGTTACTAA AATAATATCT 
GCATTGAAAG GAAAACGTGG TGACCACCGT ATATTCAGAC TAGCTGAAAA CTTATGCATG 
AATCTCATTT TATCACTCAG AGACTTTTTC TCTGTGAAAC GGGAGGGAAA AGGGCCAACT 
GAATTCACTG AGACGTTAAA TCGCATAACC ATCATGACTC TTGCTATCAC TATCAAAACA 
CGTGGTATCG CAGACCCTGA TCACATGGTT TATCTGCAAA CCATGTTGGA ACAAATACTC 
GCGACGAGTC AGCATACATG GTCAGAGAAG ACGATGCGGC ATTTCCCATC CCTTCTCCGT 
GAAACTTTGA AAGGACGGGT AGACAAGAGG GGCCTATCAA TTCAGGCATG GCAACAGGCT 
GAAACAACTG TGATAAACCA GTGCACTCAA CTTCTCTCAC CATCTGCTGA ACCCGCTTAC 
GTTTCAACAT ACCTCAGTCA CAGTTTTCCT CAACACCGTC AGTATCTATG TGCTGGTGCT 
TGTCTGCTGA TGCAAGGCCA CGCTGAAAAC ATCAACAGCA CAAATCTGGC ACGAGTTCTG 
AGAGAAGTTT CTCCTGAAGA AGTCACTGCT AACATATACA CTTTGGTCGA TGTTTTGCTT 
CACCACGTTC ATGTAGACCT TCAGCAAGGA CAATCTCTAG AGGCAGTTCT AGACAAGGCA 
GGCGCAAATC TTGCATTTTT CTTCTGGACA CATGAAATGC TTCCTCTCGA CATTTTTCTT 
TTGGCGCTCA TTGACCGTGA TGATGATCCT CACGCCTTGA TAATTGCAAT GAGCCTACTT 
AAAACGCCAG ACCTTCTGCT GAGGATAAAA AACTACTGCC AAAACCGTGG GTCTCCTGAG 
CACTGGCTTG TCACACAGGT GTTCAAACGC AATGAACTCC AGAAGGCCCT GGGTAACCAT 
CTTTCTTGGA AGGACAGGTA TCCAACGTTC TTTGATGATA TTGCAGCGCG TTTGCTTCCA 
GTTATCCCTC TAGTGCTTTA CAGGCTCATC GAGAACAATG CGATGGAGCA AGCTGATAAT 
CTTTTGCTTG CACACTCGCA TTTTCTAGCG TATCATCCTC TCAGATTCAC GTTTGTCCGT 
GATATACTAG CCTATTTCTA TGGCCATCTT CCTGGGAAAC TTGTTCTGCG CATGCTCAAA 
GTACTTGATC TCAGCAAGAT TCCATTCTCT GAATCATTCC CGCAGTACAT TAGCCCTACC 
GGTGCACCTG TATGCCCACC TCTAGATTAC TTTGCCTCTC TTCTGCTTAA TCTCGTAAAC 
AATGTTATAC CTCCACTTAG CAGCAGCAGC AACTGCAGCT CGAGGTCTGG CTCAATGGCA 
GACATCTTAA ACAGCTCAGC AAGACCTCCT CATGGAAAAA CTCCAGGAAC ATCTCAGCCT 
GGACCAGCAA ACGCCTCTGA GGGCCAGAAA GCATTCTATC AGATCCAGGA CCCAGGAACT 
TACACGCAAC TGGTTCTTGA GACAGCCGTG ATCGAGATCC TCTCGCTTCC TGTCTCTGCT 
GCTCAGATTG TCTCTTCGCT TGTCCAGATA ATTGTCAACA TACAATCGAC TCTGATTCAA 
TCTGGAAACG GGTTCCACGG CGCTGCCAAC GGGGTGGGAC AAGGGTCGGT ACTGCCAACG 
TCTCCCTCTG GAGGGAGCAC AGACTCCATG AGCGCTAGCC GGTCCACTTG TCTGATTCCT 
GGTATCAACA CAGCGAGCTT TGTCTCTAGA AGCGGTTATA CATGTCAGCA ACTGTCGTGT 
CTGTTGATTC AAGCTTGTGG ACTTCTGTTG GCTCAGCTCC CTCCAGATTT TCACGTACAG 
CTTTACTTGG AGGCAGCACG TGTGACAAGG GAAACATGGT GGCTTAAGGA CGGGAAGAGA 
TCACAAGGTG AACTAGACTC AGCTGTTGGA TACGCTTTGA TGGATCCTAC ATGGGCTGCT 
CAGGATAACA CCTCGACCGC CATAGGAAAT ATTGTCGCCT TGCTTCATGC TTTCTTCAGC 
AATCTCCCAC AAGAATGGCT TGATGGCACG AATGCTATCA TCACGAACCT CCGGCCTGTG 
ACATCTGTGG CAATGCTCAG AGTTGTATTC CGTATCATGG GGCCACTGCT TCCGAGGCTT 
GCCAGTACAC ATACTCTCTT TAACAAGACG CTAATGCTTC TCTTAAGCGC ACTGGTCGAT 
GTTTTTGGAA AGACCGCACA GACAACAGCC CCTGTTGAAG CATCCCAGAT TGCAGATCTT 
ATTGATTTCC TACACCATAT TATTCACTAC GAGGGACAAG GAGGAGCTGT TCAAACTAGC 
AGCAAGCCGA GACCGGACAT CTTGGCGTTA ATCGGAAGAG CAGCAGAGAC TCTCCGACCA 
GATGTACAAC ACCTTCTCGC TCACCTCAAA ACCAACCCGA ATTCTTCCAT ATACGCAGCT 
GCTCATCAGC AGAATACTGC AAAGACTAAC ACCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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AT1G23230 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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