HCF173 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCF173
Entrez Gene ID	838243
Full Name	high chlorophyll fluorescence phenotype 173
Synonyms	F19K19.14,high chlorophyll fluorescence phenotype 173
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001332253.1	NP_001323375.1	high chlorophyll fluorescence phenotype 173
NM_001332254.1	NP_001323376.1	high chlorophyll fluorescence phenotype 173
NM_101533.4	NP_173116.1	high chlorophyll fluorescence phenotype 173

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	HCF173	NP_173116.1
Oryza sativa (rice)	Os06g0704700	NP_001058511.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HCF244 was identified by coexpression analysis of HCF173, which encodes a protein that is also necessary for psbA translational initiation and in addition for stabilization of this messenger RNA.
Title: The atypical short-chain dehydrogenases HCF173 and HCF244 are jointly involved in translational initiation of the psbA mRNA of Arabidopsis.

The protein HCF173 is localized in the chloroplast, where it is mainly associated with the membrane system. It is part of a higher molecular weight complex and is essential for translation of the D1 encoding psbA mRNA. [HCF173]
Title: The nuclear-encoded factor HCF173 is involved in the initiation of translation of the psbA mRNA in Arabidopsis thaliana.

The following HCF173 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCF173 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb31101	NM_101533.4 Latest version!	Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb33580	NM_001332253.1 Latest version!	Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OAb33581	NM_001332254.1 Latest version!	Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCF173 ( NM_101533.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_173116.1 Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), mRNA.

Price & Availability↑

CloneID	OAb31101
Clone ID Related Accession (Same CDS sequence)	NM_101533.4
Accession Version	NM_101533.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	high chlorophyll fluorescence phenotype 173
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_101533.3.

ATGGTGGGTA GTATTGTTGG CAGTAACATG GCTGCGACTG ATGCGAGGTT TCTAAGCTCG 
AATTTTGGCA ACAGTTTCAG CATCAACACC AGAATTCACA GATTCCATGA TCGCTCCCAA 
ATCGTAATCC CTAGGGCTCA ATCTTCTTCT TCTCCGTCTC CATCTCCACC CTCCGACAAG 
AAGAAGACCA AAACCCGACC CGGAACCATA ACCACTAAGG AGAGCGAAGA GACGGTTGCG 
AAGAAGCTCG ACGTTGCTCC GCCTTCGCCT CAATCACCAC CGTCTCCGCC TACACTGAAG 
TTGGACGATG TGAATCCTGT GGGATTAGGA CGACGATCGC GGCAGATCTT CGACGAGGTG 
TGGCGGAAAT TCTCTGGATT AGGTCAGATG TCGAGGACAA CCCGACCCGA TGAGCAAGAG 
ACTCTTGATA GTCTACTCAT TAGAGAAGGA CCTATGTGTG AGTTTGCTGT TCCCGGCGCT 
CAAAACGTTA CCGTTTTAGT CGTCGGAGCT ACTAGTAGAA TCGGTCGTAT CGTTGTCCGT 
AAACTCATGC TCCGTGGCTA CACCGTCAAG GCACTGGTGA GGAAACAAGA TGAGGAAGTG 
ATGAGTATGT TGCCAAGGTC AGTGGATATT GTTGTTGGAG ATGTGGGTGA ACCGTCAACG 
CTCAAATCCG CTGTTGAAAG CTGCAGCAAG ATCATCTACT GTGCGACTGC TCGGTCTACG 
ATTACTGCAG ACCTTACGCG GGTTGATCAC TTGGGTGTTT ATAACCTCAC CAAGGCTTTT 
CAGGATTACA ATAACAGACT AGCGCAATTA AGGGCGGGTA AAAGCAGCAA AAGCAAGCTT 
CTATTAGCTA AGTTCAAGTC AGCTGAGTCA CTTGATGGTT GGGAAATTCG TCAAGGAACT 
TACTTTCAGG ACACAACCGC TTCCAAATAT GATGGTGGGA TGGATGCTAA GTTTGAGTTC 
ACTGAAACTG AGAGAGCTGA GTTTTCAGGT TATGTTTTCA CCCGAGGAGG ATATGTTGAG 
TTGTCGAAGA AACTTTCACT TCCATTGGGT ACCACTCTTG ACAGGTACGA AGGCTTAGTT 
CTTTCTGTTG GTGGGAATGG AAGATCCTAT GTTGTAATCC TTGAAGCTGG TCCATCATCA 
GATATGTCTC AGAGCAAACA GTATTTCGCT AGGATCAGTA CCAAAGCAGG GTTTTGTCGG 
GTAAGGGTGC CATTTTCAGC TTTTCGACCG GTCAACCCAG AAGATCCACC GCTAGATCCA 
TTTCTTGTTC ACACATTGAC AATACGCTTT GAGCCTAAAA GACAGAGGCC TGTTGATGGA 
CTTGCTGGTG CGCAACAAGA TTTGAGAAGT TTTAGCCTTG TATTTGAGTA CATAAAAGCT 
TTGCCTGCGG GTCAAGAAAC AGACTTTATT CTGGTATCGT GTACTGGTTC GGGAGTAGAA 
GCCAACAGAA GGGAGCAAGT GTTGAAAGCT AAGAGGGCTG GTGAAGATTC TTTAAGGAGA 
TCAGGCCTTG GATACACAAT CATTCGTCCT GGTCCCTTGA AGGAGGAACC AGGCGGGCAA 
CGAGCTCTGA TATTTGATCA AGGAAACAGA ATTTCTCAGG GCATTAGTTG CGCGGATGTG 
GCTGATATAT GTGTCAAGGC ACTGCACGAT TCAACCGCCA GAAACAAAAG CTTTGATGTT 
TGCCATGAAT ACGTTGCTGA GCAAGGAATA GAACTCTATG AGCTGGTGGC TCATTTGCCA 
GACAAGGCGA ACAACTATCT GACTCCGGCT TTATCTGTAC TTGAGAAGAA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_173116.1
CDS	271..2067
Translation	MVGSIVGSNMAATDARFLSSNFGNSFSINTRIHRFHDRSQIVIPRAQSSSSPSPSPPSDKKKTKTRPGTITTKESEETVAKKLDVAPPSPQSPPSPPTLKLDDVNPVGLGRRSRQIFDEVWRKFSGLGQMSRTTRPDEQETLDSLLIREGPMCEFAVPGAQNVTVLVVGATSRIGRIVVRKLMLRGYTVKALVRKQDEEVMSMLPRSVDIVVGDVGEPSTLKSAVESCSKIIYCATARSTITADLTRVDHLGVYNLTKAFQDYNNRLAQLRAGKSSKSKLLLAKFKSAESLDGWEIRQGTYFQDTTASKYDGGMDAKFEFTETERAEFSGYVFTRGGYVELSKKLSLPLGTTLDRYEGLVLSVGGNGRSYVVILEAGPSSDMSQSKQYFARISTKAGFCRVRVPFSAFRPVNPEDPPLDPFLVHTLTIRFEPKRQRPVDGLAGAQQDLRSFSLVFEYIKALPAGQETDFILVSCTGSGVEANRREQVLKAKRAGEDSLRRSGLGYTIIRPGPLKEEPGGQRALIFDQGNRISQGISCADVADICVKALHDSTARNKSFDVCHEYVAEQGIELYELVAHLPDKANNYLTPALSVLEKNT

Target ORF information:

RefSeq Version	NM_101533.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_101533.4

ORF Insert Sequence:

ATGGTGGGTA GTATTGTTGG CAGTAACATG GCTGCGACTG ATGCGAGGTT TCTAAGCTCG 
AATTTTGGCA ACAGTTTCAG CATCAACACC AGAATTCACA GATTCCATGA TCGCTCCCAA 
ATCGTAATCC CTAGGGCTCA ATCTTCTTCT TCTCCGTCTC CATCTCCACC CTCCGACAAG 
AAGAAGACCA AAACCCGACC CGGAACCATA ACCACTAAGG AGAGCGAAGA GACGGTTGCG 
AAGAAGCTCG ACGTTGCTCC GCCTTCGCCT CAATCACCAC CGTCTCCGCC TACACTGAAG 
TTGGACGATG TGAATCCTGT GGGATTAGGA CGACGATCGC GGCAGATCTT CGACGAGGTG 
TGGCGGAAAT TCTCTGGATT AGGTCAGATG TCGAGGACAA CCCGACCCGA TGAGCAAGAG 
ACTCTTGATA GTCTACTCAT TAGAGAAGGA CCTATGTGTG AGTTTGCTGT TCCCGGCGCT 
CAAAACGTTA CCGTTTTAGT CGTCGGAGCT ACTAGTAGAA TCGGTCGTAT CGTTGTCCGT 
AAACTCATGC TCCGTGGCTA CACCGTCAAG GCACTGGTGA GGAAACAAGA TGAGGAAGTG 
ATGAGTATGT TGCCAAGGTC AGTGGATATT GTTGTTGGAG ATGTGGGTGA ACCGTCAACG 
CTCAAATCCG CTGTTGAAAG CTGCAGCAAG ATCATCTACT GTGCGACTGC TCGGTCTACG 
ATTACTGCAG ACCTTACGCG GGTTGATCAC TTGGGTGTTT ATAACCTCAC CAAGGCTTTT 
CAGGATTACA ATAACAGACT AGCGCAATTA AGGGCGGGTA AAAGCAGCAA AAGCAAGCTT 
CTATTAGCTA AGTTCAAGTC AGCTGAGTCA CTTGATGGTT GGGAAATTCG TCAAGGAACT 
TACTTTCAGG ACACAACCGC TTCCAAATAT GATGGTGGGA TGGATGCTAA GTTTGAGTTC 
ACTGAAACTG AGAGAGCTGA GTTTTCAGGT TATGTTTTCA CCCGAGGAGG ATATGTTGAG 
TTGTCGAAGA AACTTTCACT TCCATTGGGT ACCACTCTTG ACAGGTACGA AGGCTTAGTT 
CTTTCTGTTG GTGGGAATGG AAGATCCTAT GTTGTAATCC TTGAAGCTGG TCCATCATCA 
GATATGTCTC AGAGCAAACA GTATTTCGCT AGGATCAGTA CCAAAGCAGG GTTTTGTCGG 
GTAAGGGTGC CATTTTCAGC TTTTCGACCG GTCAACCCAG AAGATCCACC GCTAGATCCA 
TTTCTTGTTC ACACATTGAC AATACGCTTT GAGCCTAAAA GACAGAGGCC TGTTGATGGA 
CTTGCTGGTG CGCAACAAGA TTTGAGAAGT TTTAGCCTTG TATTTGAGTA CATAAAAGCT 
TTGCCTGCGG GTCAAGAAAC AGACTTTATT CTGGTATCGT GTACTGGTTC GGGAGTAGAA 
GCCAACAGAA GGGAGCAAGT GTTGAAAGCT AAGAGGGCTG GTGAAGATTC TTTAAGGAGA 
TCAGGCCTTG GATACACAAT CATTCGTCCT GGTCCCTTGA AGGAGGAACC AGGCGGGCAA 
CGAGCTCTGA TATTTGATCA AGGAAACAGA ATTTCTCAGG GCATTAGTTG CGCGGATGTG 
GCTGATATAT GTGTCAAGGC ACTGCACGAT TCAACCGCCA GAAACAAAAG CTTTGATGTT 
TGCCATGAAT ACGTTGCTGA GCAAGGAATA GAACTCTATG AGCTGGTGGC TCATTTGCCA 
GACAAGGCGA ACAACTATCT GACTCCGGCT TTATCTGTAC TTGAGAAGAA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCF173 ( NM_001332253.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001323375.1 Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), partial mRNA.

Price & Availability↑

CloneID	OAb33580
Clone ID Related Accession (Same CDS sequence)	NM_001332253.1
Accession Version	NM_001332253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	high chlorophyll fluorescence phenotype 173
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). COMPLETENESS: incomplete on the 3' end.

ATGGTGGGTA GTATTGTTGG CAGTAACATG GCTGCGACTG ATGCGAGGTT TCTAAGCTCG 
AATTTTGGCA ACAGTTTCAG CATCAACACC AGAATTCACA GATTCCATGA TCGCTCCCAA 
ATCGTAATCC CTAGGGCTCA ATCTTCTTCT TCTCCGTCTC CATCTCCACC CTCCGACAAG 
AAGAAGACCA AAACCCGACC CGGAACCATA ACCACTAAGG AGAGCGAAGA GACGGTTGCG 
AAGAAGCTCG ACGTTGCTCC GCCTTCGCCT CAATCACCAC CGTCTCCGCC TACACTGAAG 
TTGGACGATG TGAATCCTGT GGGATTAGGA CGACGATCGC GGCAGATCTT CGACGAGGTG 
TGGCGGAAAT TCTCTGGATT AGGTCAGATG TCGAGGACAA CCCGACCCGA TGAGCAAGAG 
ACTCTTGATA GTCTACTCAT TAGAGAAGGA CCTATGTGTG AGTTTGCTGT TCCCGGCGCT 
CAAAACGTTA CCGTTTTAGT CGTCGGAGCT ACTAGTAGAA TCGGTCGTAT CGTTGTCCGT 
AAACTCATGC TCCGTGGCTA CACCGTCAAG GCACTGGTGA GGAAACAAGA TGAGGAAGTG 
ATGAGTATGT TGCCAAGGTC AGTGGATATT GTTGTTGGAG ATGTGGGTGA ACCGTCAACG 
CTCAAATCCG CTGTTGAAAG CTGCAGCAAG ATCATCTACT GTGCGACTGC TCGGTCTACG 
ATTACTGCAG ACCTTACGCG GGTTGATCAC TTGGGTGTTT ATAACCTCAC CAAGGCTTTT 
CAGGATTACA ATAACAGACT AGCGCAATTA AGGGCGGGTA AAAGCAGCAA AAGCAAGCTT 
CTATTAGCTA AGTTCAAGTC AGCTGAGTCA CTTGATGGTT GGGAAATTCG TCAAGGAACT 
TACTTTCAGG ACACAACCGC TTCCAAATAT GATGGTGGGA TGGATGCTAA GTTTGAGTTC 
ACTGAAACTG AGAGAGCTGA GTTTTCAGGT TATGTTTTCA CCCGAGGAGG ATATGTTGAG 
TTGTCGAAGA AACTTTCACT TCCATTGGGT ACCACTCTTG ACAGGTACGA AGGCTTAGTT 
CTTTCTGTTG GTGGGAATGG AAGATCCTAT GTTGTAATCC TTGAAGCTGG TCCATCATCA 
GATATGTCTC AGAGCAAACA GTATTTCGCT AGGATCAGTA CCAAAGCAGG GTTTTGTCGG 
GTAAGGGTGC CATTTTCAGC TTTTCGACCG GTCAACCCAG AAGATCCACC GCTAGATCCA 
TTTCTTGTTC ACACATTGAC AATACGCTTT GAGCCTAAAA GACAGAGGCC TGTTGATGGA 
CTTGCTGGTG CGCAACAAGA TTTGAGAAGT TTTAGCCTTG TATTTGAGTA CATAAAAGCT 
TTGCCTGCGG GTCAAGAAAC AGACTTTATT CTGGTATCGT GTACTGGTTC GGGAGTAGAA 
GCCAACAGAA GGGAGCAAGT GTTGAAAGCT AAGAGGGCTG GTGAAGATTC TTTAAGGAGA 
TCAGGCCTTG GATACACAAT CATTCGTCCT GGTCCCTTGA AGGAGGAACC AGGCGGGCAA 
CGAGCTCTGA TATTTGATCA AGGAAACAGA ATTTCTCAGG TTATTTCAAT CACGAAAAAC 
AAACTCAAGC CACCTAGCAA AAGTTCTCTG ATGGAGCCAC TTATAACTTC CCAATGCGCT 
ATTTTCTTTC AATTTCAGGG CATTAGTTGC GCGGATGTGG CTGATATATG TGTCAAGGCA 
CTGCACGATT CAACCGCCAG AAACAAAAGC TTTGATGTTT GCCATGAATA CGTTGCTGAG 
CAAGGAATAG AACTCTATGA GCTGGTGGCT CATTTGCCAG ACAAGGCGAA CAACTATCTG 
ACTCCGGCTT TATCTGTACT TGAGAAGAAC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001323375.1
CDS	271..2166
Translation	MVGSIVGSNMAATDARFLSSNFGNSFSINTRIHRFHDRSQIVIPRAQSSSSPSPSPPSDKKKTKTRPGTITTKESEETVAKKLDVAPPSPQSPPSPPTLKLDDVNPVGLGRRSRQIFDEVWRKFSGLGQMSRTTRPDEQETLDSLLIREGPMCEFAVPGAQNVTVLVVGATSRIGRIVVRKLMLRGYTVKALVRKQDEEVMSMLPRSVDIVVGDVGEPSTLKSAVESCSKIIYCATARSTITADLTRVDHLGVYNLTKAFQDYNNRLAQLRAGKSSKSKLLLAKFKSAESLDGWEIRQGTYFQDTTASKYDGGMDAKFEFTETERAEFSGYVFTRGGYVELSKKLSLPLGTTLDRYEGLVLSVGGNGRSYVVILEAGPSSDMSQSKQYFARISTKAGFCRVRVPFSAFRPVNPEDPPLDPFLVHTLTIRFEPKRQRPVDGLAGAQQDLRSFSLVFEYIKALPAGQETDFILVSCTGSGVEANRREQVLKAKRAGEDSLRRSGLGYTIIRPGPLKEEPGGQRALIFDQGNRISQVISITKNKLKPPSKSSLMEPLITSQCAIFFQFQGISCADVADICVKALHDSTARNKSFDVCHEYVAEQGIELYELVAHLPDKANNYLTPALSVLEKNT

Target ORF information:

RefSeq Version	NM_001332253.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001332253.1

ORF Insert Sequence:

ATGGTGGGTA GTATTGTTGG CAGTAACATG GCTGCGACTG ATGCGAGGTT TCTAAGCTCG 
AATTTTGGCA ACAGTTTCAG CATCAACACC AGAATTCACA GATTCCATGA TCGCTCCCAA 
ATCGTAATCC CTAGGGCTCA ATCTTCTTCT TCTCCGTCTC CATCTCCACC CTCCGACAAG 
AAGAAGACCA AAACCCGACC CGGAACCATA ACCACTAAGG AGAGCGAAGA GACGGTTGCG 
AAGAAGCTCG ACGTTGCTCC GCCTTCGCCT CAATCACCAC CGTCTCCGCC TACACTGAAG 
TTGGACGATG TGAATCCTGT GGGATTAGGA CGACGATCGC GGCAGATCTT CGACGAGGTG 
TGGCGGAAAT TCTCTGGATT AGGTCAGATG TCGAGGACAA CCCGACCCGA TGAGCAAGAG 
ACTCTTGATA GTCTACTCAT TAGAGAAGGA CCTATGTGTG AGTTTGCTGT TCCCGGCGCT 
CAAAACGTTA CCGTTTTAGT CGTCGGAGCT ACTAGTAGAA TCGGTCGTAT CGTTGTCCGT 
AAACTCATGC TCCGTGGCTA CACCGTCAAG GCACTGGTGA GGAAACAAGA TGAGGAAGTG 
ATGAGTATGT TGCCAAGGTC AGTGGATATT GTTGTTGGAG ATGTGGGTGA ACCGTCAACG 
CTCAAATCCG CTGTTGAAAG CTGCAGCAAG ATCATCTACT GTGCGACTGC TCGGTCTACG 
ATTACTGCAG ACCTTACGCG GGTTGATCAC TTGGGTGTTT ATAACCTCAC CAAGGCTTTT 
CAGGATTACA ATAACAGACT AGCGCAATTA AGGGCGGGTA AAAGCAGCAA AAGCAAGCTT 
CTATTAGCTA AGTTCAAGTC AGCTGAGTCA CTTGATGGTT GGGAAATTCG TCAAGGAACT 
TACTTTCAGG ACACAACCGC TTCCAAATAT GATGGTGGGA TGGATGCTAA GTTTGAGTTC 
ACTGAAACTG AGAGAGCTGA GTTTTCAGGT TATGTTTTCA CCCGAGGAGG ATATGTTGAG 
TTGTCGAAGA AACTTTCACT TCCATTGGGT ACCACTCTTG ACAGGTACGA AGGCTTAGTT 
CTTTCTGTTG GTGGGAATGG AAGATCCTAT GTTGTAATCC TTGAAGCTGG TCCATCATCA 
GATATGTCTC AGAGCAAACA GTATTTCGCT AGGATCAGTA CCAAAGCAGG GTTTTGTCGG 
GTAAGGGTGC CATTTTCAGC TTTTCGACCG GTCAACCCAG AAGATCCACC GCTAGATCCA 
TTTCTTGTTC ACACATTGAC AATACGCTTT GAGCCTAAAA GACAGAGGCC TGTTGATGGA 
CTTGCTGGTG CGCAACAAGA TTTGAGAAGT TTTAGCCTTG TATTTGAGTA CATAAAAGCT 
TTGCCTGCGG GTCAAGAAAC AGACTTTATT CTGGTATCGT GTACTGGTTC GGGAGTAGAA 
GCCAACAGAA GGGAGCAAGT GTTGAAAGCT AAGAGGGCTG GTGAAGATTC TTTAAGGAGA 
TCAGGCCTTG GATACACAAT CATTCGTCCT GGTCCCTTGA AGGAGGAACC AGGCGGGCAA 
CGAGCTCTGA TATTTGATCA AGGAAACAGA ATTTCTCAGG TTATTTCAAT CACGAAAAAC 
AAACTCAAGC CACCTAGCAA AAGTTCTCTG ATGGAGCCAC TTATAACTTC CCAATGCGCT 
ATTTTCTTTC AATTTCAGGG CATTAGTTGC GCGGATGTGG CTGATATATG TGTCAAGGCA 
CTGCACGATT CAACCGCCAG AAACAAAAGC TTTGATGTTT GCCATGAATA CGTTGCTGAG 
CAAGGAATAG AACTCTATGA GCTGGTGGCT CATTTGCCAG ACAAGGCGAA CAACTATCTG 
ACTCCGGCTT TATCTGTACT TGAGAAGAAC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCF173 ( NM_001332254.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001323376.1 Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), mRNA.

Price & Availability↑

CloneID	OAb33581
Clone ID Related Accession (Same CDS sequence)	NM_001332254.1
Accession Version	NM_001332254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	high chlorophyll fluorescence phenotype 173
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGTGGGTA GTATTGTTGG CAGTAACATG GCTGCGACTG ATGCGAGGTT TCTAAGCTCG 
AATTTTGGCA ACAGTTTCAG CATCAACACC AGAATTCACA GATTCCATGA TCGCTCCCAA 
ATCGTAATCC CTAGGGCTCA ATCTTCTTCT TCTCCGTCTC CATCTCCACC CTCCGACAAG 
AAGAAGACCA AAACCCGACC CGGAACCATA ACCACTAAGG AGAGCGAAGA GACGGTTGCG 
AAGAAGCTCG ACGTTGCTCC GCCTTCGCCT CAATCACCAC CGTCTCCGCC TACACTGAAG 
TTGGACGATG TGAATCCTGT GGGATTAGGA CGACGATCGC GGCAGATCTT CGACGAGGTG 
TGGCGGAAAT TCTCTGGATT AGGTCAGATG TCGAGGACAA CCCGACCCGA TGAGCAAGAG 
ACTCTTGATA GTCTACTCAT TAGAGAAGGA CCTATGTGTG AGTTTGCTGT TCCCGGCGCT 
CAAAACGTTA CCGTTTTAGT CGTCGGAGCT ACTAGTAGAA TCGGTCGTAT CGTTGTCCGT 
AAACTCATGC TCCGTGGCTA CACCGTCAAG GCACTGGTGA GGAAACAAGA TGAGGAAGTG 
ATGAGTATGT TGCCAAGGTC AGTGGATATT GTTGTTGGAG ATGTGGGTGA ACCGTCAACG 
CTCAAATCCG CTGTTGAAAG CTGCAGCAAG ATCATCTACT GTGCGACTGC TCGGTCTACG 
ATTACTGCAG ACCTTACGCG GGTTGATCAC TTGGGTGTTT ATAACCTCAC CAAGGCTTTT 
CAGGATTACA ATAACAGACT AGCGCAATTA AGGGCGGGTA AAAGCAGCAA AAGCAAGCTT 
CTATTAGCTA AGTTCAAGTC AGCTGAGTCA CTTGATGGTT GGGAAATTCG TCAAGGAACT 
TACTTTCAGG ACACAACCGC TTCCAAATAT GATGGTGGGA TGGATGCTAA GTTTGAGTTC 
ACTGAAACTG AGAGAGCTGA GTTTTCAGGT TATGTTTTCA CCCGAGGAGG ATATGTTGAG 
TTGTCGAAGA AACTTTCACT TCCATTGGGT ACCACTCTTG ACAGGTACGA AGGCTTAGTT 
CTTTCTGTTG GTGGGAATGG AAGATCCTAT GTTGTAATCC TTGAAGCTGG TCCATCATCA 
GATATGTCTC AGAGCAAACA GTATTTCGCT AGGATCAGTA CCAAAGCAGG GTTTTGTCGG 
GTAAGGGTGC CATTTTCAGC TTTTCGACCG GTCAACCCAG AAGATCCACC GCTAGATCCA 
TTTCTTGTTC ACACATTGAC AATACGCTTT GAGCCTAAAA GACAGAGGCC TGTTGATGGA 
CTTGCTGGTG CGCAACAAGA TTTGAGAAGT TTTAGCCTTG TATTTGAGTA CATAAAAGCT 
TTGCCTGCGG GTCAAGAAAC AGACTTTATT CTGGTATCGT GTACTGGTTC GGGAGTAGAA 
GCCAACAGAA GGGAGCAAGT GTTGAAAGCT AAGAGGGCTG GTGAAGATTC TTTAAGGAGA 
TCAGGCCTTG GATACACAAT CATTCGTCCT GGTCCCTTGA AGGAGGAACC AGGCGGGCAA 
CGAGCTCTGA TATTTGATCA AGGAAACAGA ATTTCTCAGG GCATTAGTTG CGCGGATGTG 
GCTGATATAT GTGTCAAGGC ACTGCACGAT TCAACCGCCA GAAACAAAAG CTTTGATGTG 
AGTTGCAGTT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001323376.1
CDS	244..1938
Translation	MVGSIVGSNMAATDARFLSSNFGNSFSINTRIHRFHDRSQIVIPRAQSSSSPSPSPPSDKKKTKTRPGTITTKESEETVAKKLDVAPPSPQSPPSPPTLKLDDVNPVGLGRRSRQIFDEVWRKFSGLGQMSRTTRPDEQETLDSLLIREGPMCEFAVPGAQNVTVLVVGATSRIGRIVVRKLMLRGYTVKALVRKQDEEVMSMLPRSVDIVVGDVGEPSTLKSAVESCSKIIYCATARSTITADLTRVDHLGVYNLTKAFQDYNNRLAQLRAGKSSKSKLLLAKFKSAESLDGWEIRQGTYFQDTTASKYDGGMDAKFEFTETERAEFSGYVFTRGGYVELSKKLSLPLGTTLDRYEGLVLSVGGNGRSYVVILEAGPSSDMSQSKQYFARISTKAGFCRVRVPFSAFRPVNPEDPPLDPFLVHTLTIRFEPKRQRPVDGLAGAQQDLRSFSLVFEYIKALPAGQETDFILVSCTGSGVEANRREQVLKAKRAGEDSLRRSGLGYTIIRPGPLKEEPGGQRALIFDQGNRISQGISCADVADICVKALHDSTARNKSFDVSCSL

Target ORF information:

RefSeq Version	NM_001332254.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana high chlorophyll fluorescence phenotype 173 (HCF173), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001332254.1

ORF Insert Sequence:

ATGGTGGGTA GTATTGTTGG CAGTAACATG GCTGCGACTG ATGCGAGGTT TCTAAGCTCG 
AATTTTGGCA ACAGTTTCAG CATCAACACC AGAATTCACA GATTCCATGA TCGCTCCCAA 
ATCGTAATCC CTAGGGCTCA ATCTTCTTCT TCTCCGTCTC CATCTCCACC CTCCGACAAG 
AAGAAGACCA AAACCCGACC CGGAACCATA ACCACTAAGG AGAGCGAAGA GACGGTTGCG 
AAGAAGCTCG ACGTTGCTCC GCCTTCGCCT CAATCACCAC CGTCTCCGCC TACACTGAAG 
TTGGACGATG TGAATCCTGT GGGATTAGGA CGACGATCGC GGCAGATCTT CGACGAGGTG 
TGGCGGAAAT TCTCTGGATT AGGTCAGATG TCGAGGACAA CCCGACCCGA TGAGCAAGAG 
ACTCTTGATA GTCTACTCAT TAGAGAAGGA CCTATGTGTG AGTTTGCTGT TCCCGGCGCT 
CAAAACGTTA CCGTTTTAGT CGTCGGAGCT ACTAGTAGAA TCGGTCGTAT CGTTGTCCGT 
AAACTCATGC TCCGTGGCTA CACCGTCAAG GCACTGGTGA GGAAACAAGA TGAGGAAGTG 
ATGAGTATGT TGCCAAGGTC AGTGGATATT GTTGTTGGAG ATGTGGGTGA ACCGTCAACG 
CTCAAATCCG CTGTTGAAAG CTGCAGCAAG ATCATCTACT GTGCGACTGC TCGGTCTACG 
ATTACTGCAG ACCTTACGCG GGTTGATCAC TTGGGTGTTT ATAACCTCAC CAAGGCTTTT 
CAGGATTACA ATAACAGACT AGCGCAATTA AGGGCGGGTA AAAGCAGCAA AAGCAAGCTT 
CTATTAGCTA AGTTCAAGTC AGCTGAGTCA CTTGATGGTT GGGAAATTCG TCAAGGAACT 
TACTTTCAGG ACACAACCGC TTCCAAATAT GATGGTGGGA TGGATGCTAA GTTTGAGTTC 
ACTGAAACTG AGAGAGCTGA GTTTTCAGGT TATGTTTTCA CCCGAGGAGG ATATGTTGAG 
TTGTCGAAGA AACTTTCACT TCCATTGGGT ACCACTCTTG ACAGGTACGA AGGCTTAGTT 
CTTTCTGTTG GTGGGAATGG AAGATCCTAT GTTGTAATCC TTGAAGCTGG TCCATCATCA 
GATATGTCTC AGAGCAAACA GTATTTCGCT AGGATCAGTA CCAAAGCAGG GTTTTGTCGG 
GTAAGGGTGC CATTTTCAGC TTTTCGACCG GTCAACCCAG AAGATCCACC GCTAGATCCA 
TTTCTTGTTC ACACATTGAC AATACGCTTT GAGCCTAAAA GACAGAGGCC TGTTGATGGA 
CTTGCTGGTG CGCAACAAGA TTTGAGAAGT TTTAGCCTTG TATTTGAGTA CATAAAAGCT 
TTGCCTGCGG GTCAAGAAAC AGACTTTATT CTGGTATCGT GTACTGGTTC GGGAGTAGAA 
GCCAACAGAA GGGAGCAAGT GTTGAAAGCT AAGAGGGCTG GTGAAGATTC TTTAAGGAGA 
TCAGGCCTTG GATACACAAT CATTCGTCCT GGTCCCTTGA AGGAGGAACC AGGCGGGCAA 
CGAGCTCTGA TATTTGATCA AGGAAACAGA ATTTCTCAGG GCATTAGTTG CGCGGATGTG 
GCTGATATAT GTGTCAAGGC ACTGCACGAT TCAACCGCCA GAAACAAAAG CTTTGATGTG 
AGTTGCAGTT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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HCF173 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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