MUM2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MUM2
Entrez Gene ID	836500
Full Name	Glycosyl hydrolase family 35 protein
Synonyms	BGAL6,MBK5.28,MBK5_28,MUCILAGE-MODIFIED 2,beta-galactosidase 6
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_125775.4	NP_568978.2	Glycosyl hydrolase family 35 protein

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os05g0428100	NP_001174435.1
Arabidopsis thaliana (thale cress)	BGAL16	NP_177866.2
Arabidopsis thaliana (thale cress)	MUM2	NP_568978.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LUH is allelic to MUM1, and MUM2 is a likely target of LUH regulation. The transcriptional corepressor LEUNIG (LUG) is functionally interchangeable with LUH, raising the possibility that LUH functions as a repressor during seed coat maturation.
Title: The transcriptional regulator LEUNIG_HOMOLOG regulates mucilage release from the Arabidopsis testa.

Data conclude that the MUM2/BGAL6 beta-D-galactosidase is required for maturation of rhamnogalacturonan I in seed mucilage by the removal of galactose/galactan branches, resulting in increased swelling and extrusion of the mucilage on seed hydration.
Title: A naturally occurring mutation in an Arabidopsis accession affects a beta-D-galactosidase that increases the hydrophilic potential of rhamnogalacturonan I in seed mucilage.

Results show MUM2 is localized to the cell wall and it is proposed that MUM2 is involved in modifying mucilage to allow it to expand upon hydration, establishing a link between the galactosyl side-chain structure of pectin and its physical properties.
Title: The Arabidopsis MUM2 gene encodes a beta-galactosidase required for the production of seed coat mucilage with correct hydration properties.

The following MUM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MUM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb22334	NM_125775.4 Latest version!	Arabidopsis thaliana Glycosyl hydrolase family 35 protein (MUM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MUM2 ( NM_125775.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_568978.2 Arabidopsis thaliana Glycosyl hydrolase family 35 protein (MUM2), mRNA.

Price & Availability↑

CloneID	OAb22334
Clone ID Related Accession (Same CDS sequence)	NM_125775.4
Accession Version	NM_125775.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Glycosyl hydrolase family 35 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_125775.3.

ATGGAGATGG GTCGTCTGGT CTTCGGGTTG TGTTTGATTC TTATTGTGGG TACGTTTCTT 
GAATTCTCCG GCGGTGCTAC GGCGGCGAAG GGAGTAACGT ACGACGGACG GTCTTTGATA 
ATCGATGGAC AGAGGAAGCT TCTCTTCTCC GGTTCCATCC ACTATCCTCG CAGCACACCC 
GAGATGTGGC CGTCTCTGAT AAAAAAGACA AAGGAAGGAG GCATCGATGT GATCCAAACG 
TACGTTTTCT GGAACCTCCA TGAGCCTAAG CTTGGACAGT ATGATTTTAG TGGAAGAAAC 
GATTTAGTGA AGTTTATTAA AGAAATTAGA TCACAAGGTC TCTATGTCTG TCTACGTATT 
GGACCCTTCA TCGAAGCCGA GTGGAATTAC GGGGGATTGC CATTTTGGTT ACGGGATGTA 
CCCGGTATGG TATACCGAAC CGACAATGAG CCCTTCAAGT TCCACATGCA AAAATTCACC 
GCTAAGATCG TAGATTTGAT GAAATCTGAG GGCTTATATG CTTCTCAAGG AGGTCCAATT 
ATTCTTTCTC AGATCGAGAA CGAGTATGCA AACGTGGAGG GGGCTTTCCA TGAGAAAGGA 
GCATCGTACA TCAAGTGGGC AGGTCAAATG GCGGTCGGGC TTAAGACCGG AGTGCCATGG 
ATCATGTGCA AGTCGCCCGA CGCTCCTGAT CCCGTGATCA ATACCTGCAA CGGGATGAAA 
TGCGGCGAGA CTTTTCCTGG ACCAAACTCA CCCAATAAGC CGAAAATGTG GACTGAGGAT 
TGGACTTCAT TCTTTCAGGT TTATGGAAAA GAGCCTTATA TTAGATCTGC TGAGGATATT 
GCTTTCCATG CCGCTCTTTT CGTTGCAAAA AATGGAAGTT ACATCAACTA TTACATGTAC 
CATGGTGGAA CAAACTTTGG AAGGACTTCA TCTTCGTATT TCATTACAGG ATACTATGAT 
CAAGCTCCTC TCGACGAATA CGGTCTTTTA AGACAGCCTA AGTACGGTCA TTTGAAGGAG 
CTTCACGCTG CGATAAAATC CTCTGCAAAT CCTCTGCTTC AAGGAAAGCA AACGATTCTC 
TCCTTGGGAC CAATGCAACA AGCTTATGTC TTTGAAGACG CGAACAATGG ATGCGTTGCG 
TTTCTGGTAA ACAATGACGC TAAAGCCAGT CAAATACAAT TCAGAAACAA TGCGTATAGC 
TTGAGTCCCA AGTCAATTGG TATTCTTCAG AACTGCAAAA ACCTAATTTA TGAAACTGCA 
AAAGTGAATG TGAAGATGAA TACGAGAGTT ACAACGCCGG TTCAAGTGTT CAACGTTCCC 
GATAACTGGA ATTTGTTTAG AGAAACAATC CCTGCATTCC CAGGCACATC TTTGAAGACA 
AATGCTTTGT TGGAACACAC TAATTTGACA AAGGACAAAA CTGATTATCT TTGGTACACT 
TCAAGCTTTA AGCTGGATTC ACCCTGCACG AACCCGTCTA TCTACACAGA ATCATCAGGA 
CATGTTGTCC ACGTGTTCGT CAACAATGCA CTAGCAGGAT CTGGACATGG AAGCAGAGAC 
ATAAGGGTAG TTAAACTACA AGCTCCGGTT AGCTTAATCA ATGGACAAAA CAATATATCG 
ATCCTCAGCG GCATGGTTGG TCTACCGGAC TCTGGAGCTT ACATGGAGAG GAGATCTTAT 
GGACTAACCA AAGTACAAAT TAGCTGTGGC GGGACAAAAC CCATCGATTT GAGTAGATCT 
CAATGGGGTT ACTCGGTGGG TTTGCTTGGA GAGAAAGTTA GGTTATATCA ATGGAAGAAT 
TTAAATAGAG TGAAGTGGAG TATGAATAAA GCTGGACTAA TTAAGAACCG CCCCCTCGCT 
TGGTACAAGA CAACGTTTGA TGGGCCGAAT GGAGATGGGC CCGTGGGCCT ACACATGTCG 
AGTATGGGTA AAGGAGAAAT TTGGGTGAAT GGTGAAAGCA TTGGTCGTTA TTGGGTCTCG 
TTTCTCACCC CTGCTGGACA ACCTTCTCAA TCTATATATC ATATTCCTCG GGCGTTCTTA 
AAACCATCTG GAAATTTATT GGTGGTATTT GAAGAGGAAG GTGGTGATCC TCTTGGGATA 
TCTTTGAATA CAATATCGGT TGTTGGTTCG AGTCAAGCTC AATCTCAATT CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_568978.2
CDS	269..2425
Translation	MEMGRLVFGLCLILIVGTFLEFSGGATAAKGVTYDGRSLIIDGQRKLLFSGSIHYPRSTPEMWPSLIKKTKEGGIDVIQTYVFWNLHEPKLGQYDFSGRNDLVKFIKEIRSQGLYVCLRIGPFIEAEWNYGGLPFWLRDVPGMVYRTDNEPFKFHMQKFTAKIVDLMKSEGLYASQGGPIILSQIENEYANVEGAFHEKGASYIKWAGQMAVGLKTGVPWIMCKSPDAPDPVINTCNGMKCGETFPGPNSPNKPKMWTEDWTSFFQVYGKEPYIRSAEDIAFHAALFVAKNGSYINYYMYHGGTNFGRTSSSYFITGYYDQAPLDEYGLLRQPKYGHLKELHAAIKSSANPLLQGKQTILSLGPMQQAYVFEDANNGCVAFLVNNDAKASQIQFRNNAYSLSPKSIGILQNCKNLIYETAKVNVKMNTRVTTPVQVFNVPDNWNLFRETIPAFPGTSLKTNALLEHTNLTKDKTDYLWYTSSFKLDSPCTNPSIYTESSGHVVHVFVNNALAGSGHGSRDIRVVKLQAPVSLINGQNNISILSGMVGLPDSGAYMERRSYGLTKVQISCGGTKPIDLSRSQWGYSVGLLGEKVRLYQWKNLNRVKWSMNKAGLIKNRPLAWYKTTFDGPNGDGPVGLHMSSMGKGEIWVNGESIGRYWVSFLTPAGQPSQSIYHIPRAFLKPSGNLLVVFEEEGGDPLGISLNTISVVGSSQAQSQFS

Target ORF information:

RefSeq Version	NM_125775.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Glycosyl hydrolase family 35 protein (MUM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_125775.4

ORF Insert Sequence:

ATGGAGATGG GTCGTCTGGT CTTCGGGTTG TGTTTGATTC TTATTGTGGG TACGTTTCTT 
GAATTCTCCG GCGGTGCTAC GGCGGCGAAG GGAGTAACGT ACGACGGACG GTCTTTGATA 
ATCGATGGAC AGAGGAAGCT TCTCTTCTCC GGTTCCATCC ACTATCCTCG CAGCACACCC 
GAGATGTGGC CGTCTCTGAT AAAAAAGACA AAGGAAGGAG GCATCGATGT GATCCAAACG 
TACGTTTTCT GGAACCTCCA TGAGCCTAAG CTTGGACAGT ATGATTTTAG TGGAAGAAAC 
GATTTAGTGA AGTTTATTAA AGAAATTAGA TCACAAGGTC TCTATGTCTG TCTACGTATT 
GGACCCTTCA TCGAAGCCGA GTGGAATTAC GGGGGATTGC CATTTTGGTT ACGGGATGTA 
CCCGGTATGG TATACCGAAC CGACAATGAG CCCTTCAAGT TCCACATGCA AAAATTCACC 
GCTAAGATCG TAGATTTGAT GAAATCTGAG GGCTTATATG CTTCTCAAGG AGGTCCAATT 
ATTCTTTCTC AGATCGAGAA CGAGTATGCA AACGTGGAGG GGGCTTTCCA TGAGAAAGGA 
GCATCGTACA TCAAGTGGGC AGGTCAAATG GCGGTCGGGC TTAAGACCGG AGTGCCATGG 
ATCATGTGCA AGTCGCCCGA CGCTCCTGAT CCCGTGATCA ATACCTGCAA CGGGATGAAA 
TGCGGCGAGA CTTTTCCTGG ACCAAACTCA CCCAATAAGC CGAAAATGTG GACTGAGGAT 
TGGACTTCAT TCTTTCAGGT TTATGGAAAA GAGCCTTATA TTAGATCTGC TGAGGATATT 
GCTTTCCATG CCGCTCTTTT CGTTGCAAAA AATGGAAGTT ACATCAACTA TTACATGTAC 
CATGGTGGAA CAAACTTTGG AAGGACTTCA TCTTCGTATT TCATTACAGG ATACTATGAT 
CAAGCTCCTC TCGACGAATA CGGTCTTTTA AGACAGCCTA AGTACGGTCA TTTGAAGGAG 
CTTCACGCTG CGATAAAATC CTCTGCAAAT CCTCTGCTTC AAGGAAAGCA AACGATTCTC 
TCCTTGGGAC CAATGCAACA AGCTTATGTC TTTGAAGACG CGAACAATGG ATGCGTTGCG 
TTTCTGGTAA ACAATGACGC TAAAGCCAGT CAAATACAAT TCAGAAACAA TGCGTATAGC 
TTGAGTCCCA AGTCAATTGG TATTCTTCAG AACTGCAAAA ACCTAATTTA TGAAACTGCA 
AAAGTGAATG TGAAGATGAA TACGAGAGTT ACAACGCCGG TTCAAGTGTT CAACGTTCCC 
GATAACTGGA ATTTGTTTAG AGAAACAATC CCTGCATTCC CAGGCACATC TTTGAAGACA 
AATGCTTTGT TGGAACACAC TAATTTGACA AAGGACAAAA CTGATTATCT TTGGTACACT 
TCAAGCTTTA AGCTGGATTC ACCCTGCACG AACCCGTCTA TCTACACAGA ATCATCAGGA 
CATGTTGTCC ACGTGTTCGT CAACAATGCA CTAGCAGGAT CTGGACATGG AAGCAGAGAC 
ATAAGGGTAG TTAAACTACA AGCTCCGGTT AGCTTAATCA ATGGACAAAA CAATATATCG 
ATCCTCAGCG GCATGGTTGG TCTACCGGAC TCTGGAGCTT ACATGGAGAG GAGATCTTAT 
GGACTAACCA AAGTACAAAT TAGCTGTGGC GGGACAAAAC CCATCGATTT GAGTAGATCT 
CAATGGGGTT ACTCGGTGGG TTTGCTTGGA GAGAAAGTTA GGTTATATCA ATGGAAGAAT 
TTAAATAGAG TGAAGTGGAG TATGAATAAA GCTGGACTAA TTAAGAACCG CCCCCTCGCT 
TGGTACAAGA CAACGTTTGA TGGGCCGAAT GGAGATGGGC CCGTGGGCCT ACACATGTCG 
AGTATGGGTA AAGGAGAAAT TTGGGTGAAT GGTGAAAGCA TTGGTCGTTA TTGGGTCTCG 
TTTCTCACCC CTGCTGGACA ACCTTCTCAA TCTATATATC ATATTCCTCG GGCGTTCTTA 
AAACCATCTG GAAATTTATT GGTGGTATTT GAAGAGGAAG GTGGTGATCC TCTTGGGATA 
TCTTTGAATA CAATATCGGT TGTTGGTTCG AGTCAAGCTC AATCTCAATT CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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MUM2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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