AT5G61140 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT5G61140
Entrez Gene ID	836235
Full Name	U5 small nuclear ribonucleoprotein helicase
Synonyms	MAF19.14,MAF19_14
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_125507.2	NP_200922.2	U5 small nuclear ribonucleoprotein helicase
NM_001203655.2	NP_001190584.1	U5 small nuclear ribonucleoprotein helicase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ascc3	XP_002664072.3
Magnaporthe oryzae (rice blast fungus)	MGG_06124	XP_003711991.1
Drosophila melanogaster (fruit fly)	CG5205	NP_650472.2
Saccharomyces cerevisiae (baker's yeast)	SLH1	NP_011787.4
Neurospora crassa	NCU08267	XP_961895.1
Oryza sativa (rice)	Os03g0213400	NP_001049362.1
Bos taurus (cattle)	ASCC3	NP_001193047.1
Mus musculus (house mouse)	Ascc3	NP_932124.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001234	XP_321922.5
Arabidopsis thaliana (thale cress)	AT5G61140	NP_001190584.1
Canis lupus familiaris (dog)	ASCC3	XP_854167.1
Gallus gallus (chicken)	ASCC3	XP_419816.1
Kluyveromyces lactis	KLLA0A09889g	XP_451432.1
Eremothecium gossypii	AGOS_ABR142W	NP_983089.2
Schizosaccharomyces pombe (fission yeast)	mug81	NP_596560.1
Homo sapiens (human)	ASCC3	NP_006819.2
Pan troglodytes (chimpanzee)	ASCC3	XP_518652.3
	ASCC3	XP_002803889.1
Rattus norvegicus (Norway rat)	Ascc3	NP_001263986.1
Caenorhabditis elegans (roundworm)	CELE_Y54E2A.4	NP_497060.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT5G61140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT5G61140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb22076	NM_125507.2 Latest version!	Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (AT5G61140), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb29763	NM_001203655.2 Latest version!	Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (AT5G61140), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT5G61140 ( NM_125507.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200922.2 Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (AT5G61140), partial mRNA.

Price & Availability↑

CloneID	OAb22076
Clone ID Related Accession (Same CDS sequence)	NM_125507.2
Accession Version	NM_125507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	U5 small nuclear ribonucleoprotein helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On May 13, 2003 this sequence version replaced NM_125507.1. COMPLETENESS: incomplete on the 5' end.

ATGGTCCACA ACTCCCCATG TAATAAACAT AAAACGACGT CGATTTCGTG GACCCGTATC 
TTAGCGGGTC GGTGTACGGG CCTATTTGTT CAAGTATCTA ACCGACGCCG TTTTAGAACG 
CAAGCGATCG CCTTCTTCCT CGGAGAGCCG ACCAGAGGTG AAAACACCAC AGAGGAGAAG 
AATCCTAAAC GACACAAGTA CAGGGATTTT GTCATGTTAG TTCAGCTTCC TCGCTTGACA 
AGCTCTCTTA GAGAGCCTTT CGACATCGAT CAGGCTTACC TTCGCCGCAA AACTATACTC 
CAAACTCTTA ATAAGCCTCG CAGCTCCGGT AATCGCCTTG ATGAATCTGA CCTTGCTAAG 
AGGATAGTTC ATCAATGGGA AGGAGCTTCT CTCGAAGTGC GACAAGCATA CAAGCAGTTT 
ATTGGAGCTG TAGTGGAATT GATTGATCGG GAAGTTCCAT CAGATGAGTT TCGCGAGGTT 
GCTTTTTCCG CTTATCGTCT GTTTAACAAT CCAGTGGAGG AAGATGATAG TGATATCAAT 
GATAATATAA GCATTTCCGG GAAGAAGTTA GAGTTGCAAA ATCTTGTCGG GCATGCTGTC 
TCAGATGCTA ATGTAAAAAA CGTGGCCTCC TTTGCTCAGG CACTTTACTC AATACAGCCT 
ACTCATCAGT CTGAGACATA TGCTGATGAA GTTAATGGGG GAGCTGAGTT TGGTGCCGAC 
CTTGTTTTCA ATCTACCAGC TCGTTTTTTG GTGGAAGCAT CTCTTGACGA AACTGGGTTT 
GTGGATGTAG AAAGCAATGA TGCTCATACA TCGTTTTCTG AAGGATGGAG TGGTGTTAGC 
GACACGAAAA ATAATCTCAG TGCAGGAAAA TTCAATTTAA GTTGGCTAAG AGATGCATGT 
GGTCGGATGG TTAGAGAAAC CAATTCACAA CTTTCCCGTG AAGAATTGGC TATGGCTATA 
TGCAGATTTC TGGATTCGGA TAAACCCGGT GAGGAGATAG CTGGGGATCT GCTGGATCTT 
GTTGGGGATG GTGCATTTGA GACTGTTCAA GACCTTATTA TGCATCGGAA AGAAATTGTT 
GATGCTATCC ATCATGGCCA AATGATTCTC AAGTCTGACA AAGCAGCTTC AAACACCCAA 
TCTCGGATGC CTACCTATGG AACACAGGTT ACTGTTCAAA CAGAGTCTGC AAAGCAAATT 
GAAAAACTTC GGCGTAAGGA GGAAAAGAAA AATAAACGAA ATGCTGATCT TGGTTTAGAG 
AGTGAAATAT CAGAGGCCAA TTTTTCTTCT TTACTTGAGG CAAGTGAAAA GAAGACTGCA 
TTTGAGGATC TGATTGGGTC TGGAGAAGCG AATTCTCTTG CACTTGCCCT TCCCCAAGGT 
ACTGTAAGGA AGCACTTAAA AGGATATGAG GAAGTGTTTA TCCCGCCAAC CCCTACTGCA 
CAAATGAAAC CTGGCGAGAA GCTGATTGAG ATAAAAGAGT TAGATGACTT TGCTCAAGCT 
GCTTTTCATG GTTACAAGTC TTTAAACCGT ATTCAGAGTC GCATATTTCA GACAGTTTAC 
CACACCAATG AGAATATATT GGTCTGTGCT CCAACAGGAG CTGGTAAAAC AAATATAGCT 
ATGATTTCTG TTCTACATGA GATCAAACAA CACTTCAGGG ATGGCTACTT GCACAAAAAT 
GAGTTTAAGA TTGTTTATGT AGCTCCTATG AAGGCGTTAG CAGCAGAGGT CACGTCAGCA 
TTTAGCCGTC GTCTAGCTCC ATTAAATATG GTTGTGAAGG AGCTTACAGG GGATATGCAA 
CTCACGAAGA CTGAACTTGA AGAAACTCAG ATGATAGTGA CAACACCAGA GAAATGGGAT 
GTCATTACTC GCAAAAGCAG TGACATGTCA ATGTCAATGC TAGTAAAACT ATTGATCATT 
GATGAAGTGC ATCTTCTGAA TGATGATAGA GGTGCAGTGA TAGAGGCGCT AGTTGCTCGG 
ACCCTCCGTC AGGTGGAGTC AACTCAGACA ATGATAAGAA TTGTAGGCCT GTCAGCCACT 
TTACCTAGCT ACCTACAGGT TGCTCAGTTT CTACGAGTGA ATACAGACAC TGGCCTATTC 
TATTTTGATT CAAGTTATCG CCCAGTTCCC TTAGCTCAAC AATACATAGG CATCACTGAG 
CATAACTTTG CTGCTAGAAA CGAATTATTA AATGAGATAT GCTACAAAAA GGTTGTTGAT 
TCAATTAAAC AAGGTCATCA AGCAATGATA TTTGTTCATT CCCGAAAAGA CACTTCAAAA 
ACAGCTGAGA AACTGGTTGA TCTTGCCCGG CAATATGAAA CTCTGGATTT GTTCACGAAT 
GAGACTCATC CTCAATTTCA ACTGATGAAG AAAGACGTTA TGAAGTCCAG AAATAAGGAT 
CTTGTCAAAT TCTTTGAAGC TGGCTTCGGA ATTCATCATG CTGGGATGTT GAGATCTGAT 
AGAACACTTA CGGAGAGGCT GTTTTCTGAT GGTCTCTTGA AGGTGCTTGT TTGCACTGCA 
ACTCTTGCGT GGGGTGTAAA TCTGCCTGCA CACACAGTCG TAATTAAGGG AACACAATTG 
TATGATGCCA AGGCTGGTGG GTGGAAAGAC TTGGGAATGC TTGATGTCAT GCAGATCTTT 
GGAAGGGCAG GGAGACCACA ATTTGATAAG AGTGGTGAGG GAATTATCAT CACATCCCAT 
GACAAGCTGG CATACTATCT CCGGCTGCTG ACTAGTCAGC TTCCCATAGA AAGTCAGTTC 
ATAAGCTCCT TGAAAGATAA CTTAAACGCA GAAGTGGTTT TGGGAACTGT AACTAATGTA 
AAGGAAGCCT GTGCTTGGCT TGGATATACA TATTTGTCAA TTCGGATGAA GTTAAATCCT 
TTGGCTTATG GCATTGGATG GGAAGAGATA ATTGCCGATC CTTCTTTATC CTTGAAGCAA 
CGAGCCCTTG TTGCTGATGC TGCCCGTTCA CTGGACAAAG CTAAGATGAT GAGGTTCGAT 
GAGAAAAGTG GAAACTTTTA CTGTACCGAG CTTGGACGGG TTGCAAGTCA CTTCTATATT 
CAATATTCTA GTGTTGAAAC ATACAACGAA ATGTTGAAGC GGCACATGAA TGAAAGCGAG 
ATAATTAATA TGGTTGCCCA TTCTTCTGAA TTTGAAAATA TTGTGGTAAG AGAGGAAGAG 
CAGCATGAAC TTGAGACTCT TGCACGCTCT TGCTGTCCCT TAGAAGTTAA GGGAGGCCCT 
TCTAACAAAC ATGGAAAAAT TTCGATTCTT ATTCAGTTGT ACATATCCCG TGGATCAATT 
GATGCATTTT CTCTGGTCTC TGATGCTTCA TACATTAGTG CTAGTTTAGC ACGTATAATG 
CGTGCTCTGT TTGAAATATG CTTGCGAAAA GGCTGGTGTG AGATGACTTT ATTTATGTTG 
GAATACTGCA AGGCAGTTGA TCGTCAACTA TGGCCTCATC AGCATCCTCT TCGGCAATTC 
GAAAGGGATC TGCCTTCTGA TCGAAGGGAT GACTTAGACC ATTTGTATGA GATGGAGGAA 
AAGGAAATTG GAGCGCTGAT TCGTTATAAT CCTGGGGGAA GGCATCTAGG ATATTTTCCT 
TCAATTCAGT TGGCAGCTAC TGTTAGTCCA ATCACCCGCA CTGTATTGAA GGTGGATTTG 
CTTATAACTC CAAATTTCAT ATGGAAGGAT CGTTTTCATG GTACTGCTCT TCGTTGGTGG 
ATTCTCATTG AGGACACAGA GAATGATTAT ATCTATCACT CAGATCTTTT CACACTAACG 
AAGCGGATGG CTAGAGGGGA ACCACAGAAG CTTTCTTTTA CCGTACCGAT ATTTGAACCA 
CACCCACCAC AATACTATGT TCATGCGGTT TCTGATTCTT GGTTGCATGC AGAGACTTAC 
TTCACAATCT CTTTTCACAA TCTTGCACTA CCAGAGGCGC GGACGTCTCA CACAGAGCTT 
TTAGACTTAA AACCTCTTCC TGTGACTTCT CTTGGGAATA AGTTGTATGA ATCGCTTTAC 
AAATTTTCAC ATTTCAATCC AATACAGACT CAGATTTTTC ATGTGTTATA TCACACAGAC 
AACAATGTTC TTGTAGGAGC TCCAACTGGA AGTGGGAAAA CTATATCTGC TGAACTTGCA 
ATGTTGCGTC TCTTCAGTAC CCAGCCTGAT ATGAAGGTTG TTTACATAGC ACCACTGAAG 
GCTATTGTTC GAGAAAGGAT GAATGATTGG AAAAAGCATC TTGTTGCTCC ACTTGGAAAA 
GAAATGGTTG AAATGACTGG AGATTACACT CCAGATTTAG TAGCTCTCTT GTCCGCGGAC 
ATCATCATAT CTACCCCTGA AAAATGGGAT GGCATTAGTC GTAATTGGCA TACTCGAAGT 
TATGTCAAAA AGGTCGGCCT TGTTATTTTG GATGAGATTC ACTTGCTTGG AGCCGACAGA 
GGACCGATTC TTGAGGTTAT TGTATCTAGG ATGAGATATA TATCATCACA GACAGAGCGC 
TCCGTCCGGT TTGTTGGGCT TTCTACAGCT CTGGCAAATG CAGGTGATTT GGCTGACTGG 
TTGGGTGTTG GGGAGATCGG ACTTTTCAAT TTTAAACCAA GCGTCAGGCC TGTACCAATT 
GAAGTTCATA TTCAGGGTTA TCCTGGAAAG TATTACTGCC CAAGGATGAA TAGCATGAAC 
AAGCCAGCAT ATGCAGCCAT ATGTACTCAT TCACCTACGA AACCTGTTCT TATCTTTGTT 
TCATCCCGTA GGCAGACAAG GCTTACTGCA TTGGATCTTA TTCAGTTTGC TGCCTCCGAC 
GAACATCCGC GGCAATTTCT GAGTGTGTCA GAGGAAGACT TGCAGATGGT CCTCTCACAG 
ATAACTGATC AAAACCTTCG ACATACGTTG CAATTCGGCA TCGGACTGCA TCATGCAGGT 
TTAAACGACC ACGATAGATC TGCAGTCGAA GAGCTTTTCA CGAACAACAA GATTCAGGTT 
TTGGTCTCAA CAAGTACCTT GGCATGGGGA GTGAACCTAC CAGCTCACTT AGTTATTATA 
AAGGGAACAG AATATTTCGA CGGGAAAACC AAAAGATATG TTGACTTCCC TCTTACAGAG 
ATCTTGCAAA TGATGGGACG TGCAGGGCGA CCACAGTTTG ACCAACATGG GAAAGCGGTT 
ATTCTTGTAC ATGAACCTAA AAAAAGCTTT TACAAAAAGT TCTTGTACGA ACCATTCCCC 
GTAGAAAGTA GTTTGAAAGA GAAGCTGCAC GATCACTTCA ATGCGGAAAT CGTCTCTGGA 
ACAATAGGCA ACAAAGAAGA TGCAGTGCAT TATCTTACAT GGACGTATTT GTTTCGTAGA 
CTGATGGCTA ATCCTGCTTA CTATGGGCTA GAAGGTACTC AAGACGAAAC AATCTGTTCC 
TACTTATCAA GATTGGTGCA AACCACATTT GAGGATCTTG AAGACAGTGG TTGCCTCAAA 
GTAAATGAAG ATAGTGTGGA GCCTACGATG CTGGGTACAA TAGCGTCACA GTATTACCTT 
TGCTACATGA CCGTGTCTAT GTTTGGCTCT AATATAGGTC CTGACACATC GCTTGAAGCA 
TTTTTGCATA TCTTGGCTGG TGCTTCTGAG TATGATGAAC TTCCTGTGCG GCACAATGAG 
GAAAATTACA ATAAAACACT GTCAGATAGA GTTCGGTATC CCGTGGATAA TAACCATCTA 
GATGATCCTC ATGTGAAAGC GAATCTGCTC TTCCAGGCAC ATTTTTCTCA ATTGGCACTT 
CCAATCAGTG ATTATAACAC GGATTTAAAG TCAGTACTGG ATCAAAGCAT TCGGATCCTC 
CAGGCCATGA TTGATATCTG CGCAAACAGT GGATGGCTCT CAAGCTCACT GACGTGTATG 
CGCCTTTTGC AAATGGTCAT GCAGGGCATG TGGTCTGATC AAGATTCGTC ATTATGGATG 
ATCCCATGCA TGAATGACCT TCTTCTAGGA TCTCTGACCG CAAGAGGGAT TCACACGCTA 
CATCAGCTAT TAAATCTTCC AAGGGAAACT CTACAAAGTG TAACCGAAAA CTTCCCTGCA 
TCAAGATTAT CTCAGGATCT CCAACGATTC CCACGGATCC AAATGAATGT GAGACTCCAA 
AAGAAGGATT CCGATGGCAA GAAAAAGCCT TCAACGCTGG AAATCAGATT GGAAAAGACA 
TCAAAGAGGA ATAGCTCGAG GGCATTGGCC CCTCGATTCC CAAAGGTGAA AGACGAAGCG 
TGGTGGTTGG TTCTTGGTGA TACTTCAACT TCTGAGCTCT TTGCTGTGAA GAGAGTTTCG 
TTCACTGGTC GTCTGATCAC ACGCATGGAG TTACCTCCCA ATATAACCTC TTTTCAGGAT 
ACGAAACTCA TCTTAGTTTC GGATTGTTAT TTGGGGTTTG AACAAGAACA CTCCATTGAA 
CAACTTGCAC GACGCGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200922.2
CDS	1..6441
Translation	MVHNSPCNKHKTTSISWTRILAGRCTGLFVQVSNRRRFRTQAIAFFLGEPTRGENTTEEKNPKRHKYRDFVMLVQLPRLTSSLREPFDIDQAYLRRKTILQTLNKPRSSGNRLDESDLAKRIVHQWEGASLEVRQAYKQFIGAVVELIDREVPSDEFREVAFSAYRLFNNPVEEDDSDINDNISISGKKLELQNLVGHAVSDANVKNVASFAQALYSIQPTHQSETYADEVNGGAEFGADLVFNLPARFLVEASLDETGFVDVESNDAHTSFSEGWSGVSDTKNNLSAGKFNLSWLRDACGRMVRETNSQLSREELAMAICRFLDSDKPGEEIAGDLLDLVGDGAFETVQDLIMHRKEIVDAIHHGQMILKSDKAASNTQSRMPTYGTQVTVQTESAKQIEKLRRKEEKKNKRNADLGLESEISEANFSSLLEASEKKTAFEDLIGSGEANSLALALPQGTVRKHLKGYEEVFIPPTPTAQMKPGEKLIEIKELDDFAQAAFHGYKSLNRIQSRIFQTVYHTNENILVCAPTGAGKTNIAMISVLHEIKQHFRDGYLHKNEFKIVYVAPMKALAAEVTSAFSRRLAPLNMVVKELTGDMQLTKTELEETQMIVTTPEKWDVITRKSSDMSMSMLVKLLIIDEVHLLNDDRGAVIEALVARTLRQVESTQTMIRIVGLSATLPSYLQVAQFLRVNTDTGLFYFDSSYRPVPLAQQYIGITEHNFAARNELLNEICYKKVVDSIKQGHQAMIFVHSRKDTSKTAEKLVDLARQYETLDLFTNETHPQFQLMKKDVMKSRNKDLVKFFEAGFGIHHAGMLRSDRTLTERLFSDGLLKVLVCTATLAWGVNLPAHTVVIKGTQLYDAKAGGWKDLGMLDVMQIFGRAGRPQFDKSGEGIIITSHDKLAYYLRLLTSQLPIESQFISSLKDNLNAEVVLGTVTNVKEACAWLGYTYLSIRMKLNPLAYGIGWEEIIADPSLSLKQRALVADAARSLDKAKMMRFDEKSGNFYCTELGRVASHFYIQYSSVETYNEMLKRHMNESEIINMVAHSSEFENIVVREEEQHELETLARSCCPLEVKGGPSNKHGKISILIQLYISRGSIDAFSLVSDASYISASLARIMRALFEICLRKGWCEMTLFMLEYCKAVDRQLWPHQHPLRQFERDLPSDRRDDLDHLYEMEEKEIGALIRYNPGGRHLGYFPSIQLAATVSPITRTVLKVDLLITPNFIWKDRFHGTALRWWILIEDTENDYIYHSDLFTLTKRMARGEPQKLSFTVPIFEPHPPQYYVHAVSDSWLHAETYFTISFHNLALPEARTSHTELLDLKPLPVTSLGNKLYESLYKFSHFNPIQTQIFHVLYHTDNNVLVGAPTGSGKTISAELAMLRLFSTQPDMKVVYIAPLKAIVRERMNDWKKHLVAPLGKEMVEMTGDYTPDLVALLSADIIISTPEKWDGISRNWHTRSYVKKVGLVILDEIHLLGADRGPILEVIVSRMRYISSQTERSVRFVGLSTALANAGDLADWLGVGEIGLFNFKPSVRPVPIEVHIQGYPGKYYCPRMNSMNKPAYAAICTHSPTKPVLIFVSSRRQTRLTALDLIQFAASDEHPRQFLSVSEEDLQMVLSQITDQNLRHTLQFGIGLHHAGLNDHDRSAVEELFTNNKIQVLVSTSTLAWGVNLPAHLVIIKGTEYFDGKTKRYVDFPLTEILQMMGRAGRPQFDQHGKAVILVHEPKKSFYKKFLYEPFPVESSLKEKLHDHFNAEIVSGTIGNKEDAVHYLTWTYLFRRLMANPAYYGLEGTQDETICSYLSRLVQTTFEDLEDSGCLKVNEDSVEPTMLGTIASQYYLCYMTVSMFGSNIGPDTSLEAFLHILAGASEYDELPVRHNEENYNKTLSDRVRYPVDNNHLDDPHVKANLLFQAHFSQLALPISDYNTDLKSVLDQSIRILQAMIDICANSGWLSSSLTCMRLLQMVMQGMWSDQDSSLWMIPCMNDLLLGSLTARGIHTLHQLLNLPRETLQSVTENFPASRLSQDLQRFPRIQMNVRLQKKDSDGKKKPSTLEIRLEKTSKRNSSRALAPRFPKVKDEAWWLVLGDTSTSELFAVKRVSFTGRLITRMELPPNITSFQDTKLILVSDCYLGFEQEHSIEQLARRG

Target ORF information:

RefSeq Version	NM_125507.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (AT5G61140), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_125507.2

ORF Insert Sequence:

ATGGTCCACA ACTCCCCATG TAATAAACAT AAAACGACGT CGATTTCGTG GACCCGTATC 
TTAGCGGGTC GGTGTACGGG CCTATTTGTT CAAGTATCTA ACCGACGCCG TTTTAGAACG 
CAAGCGATCG CCTTCTTCCT CGGAGAGCCG ACCAGAGGTG AAAACACCAC AGAGGAGAAG 
AATCCTAAAC GACACAAGTA CAGGGATTTT GTCATGTTAG TTCAGCTTCC TCGCTTGACA 
AGCTCTCTTA GAGAGCCTTT CGACATCGAT CAGGCTTACC TTCGCCGCAA AACTATACTC 
CAAACTCTTA ATAAGCCTCG CAGCTCCGGT AATCGCCTTG ATGAATCTGA CCTTGCTAAG 
AGGATAGTTC ATCAATGGGA AGGAGCTTCT CTCGAAGTGC GACAAGCATA CAAGCAGTTT 
ATTGGAGCTG TAGTGGAATT GATTGATCGG GAAGTTCCAT CAGATGAGTT TCGCGAGGTT 
GCTTTTTCCG CTTATCGTCT GTTTAACAAT CCAGTGGAGG AAGATGATAG TGATATCAAT 
GATAATATAA GCATTTCCGG GAAGAAGTTA GAGTTGCAAA ATCTTGTCGG GCATGCTGTC 
TCAGATGCTA ATGTAAAAAA CGTGGCCTCC TTTGCTCAGG CACTTTACTC AATACAGCCT 
ACTCATCAGT CTGAGACATA TGCTGATGAA GTTAATGGGG GAGCTGAGTT TGGTGCCGAC 
CTTGTTTTCA ATCTACCAGC TCGTTTTTTG GTGGAAGCAT CTCTTGACGA AACTGGGTTT 
GTGGATGTAG AAAGCAATGA TGCTCATACA TCGTTTTCTG AAGGATGGAG TGGTGTTAGC 
GACACGAAAA ATAATCTCAG TGCAGGAAAA TTCAATTTAA GTTGGCTAAG AGATGCATGT 
GGTCGGATGG TTAGAGAAAC CAATTCACAA CTTTCCCGTG AAGAATTGGC TATGGCTATA 
TGCAGATTTC TGGATTCGGA TAAACCCGGT GAGGAGATAG CTGGGGATCT GCTGGATCTT 
GTTGGGGATG GTGCATTTGA GACTGTTCAA GACCTTATTA TGCATCGGAA AGAAATTGTT 
GATGCTATCC ATCATGGCCA AATGATTCTC AAGTCTGACA AAGCAGCTTC AAACACCCAA 
TCTCGGATGC CTACCTATGG AACACAGGTT ACTGTTCAAA CAGAGTCTGC AAAGCAAATT 
GAAAAACTTC GGCGTAAGGA GGAAAAGAAA AATAAACGAA ATGCTGATCT TGGTTTAGAG 
AGTGAAATAT CAGAGGCCAA TTTTTCTTCT TTACTTGAGG CAAGTGAAAA GAAGACTGCA 
TTTGAGGATC TGATTGGGTC TGGAGAAGCG AATTCTCTTG CACTTGCCCT TCCCCAAGGT 
ACTGTAAGGA AGCACTTAAA AGGATATGAG GAAGTGTTTA TCCCGCCAAC CCCTACTGCA 
CAAATGAAAC CTGGCGAGAA GCTGATTGAG ATAAAAGAGT TAGATGACTT TGCTCAAGCT 
GCTTTTCATG GTTACAAGTC TTTAAACCGT ATTCAGAGTC GCATATTTCA GACAGTTTAC 
CACACCAATG AGAATATATT GGTCTGTGCT CCAACAGGAG CTGGTAAAAC AAATATAGCT 
ATGATTTCTG TTCTACATGA GATCAAACAA CACTTCAGGG ATGGCTACTT GCACAAAAAT 
GAGTTTAAGA TTGTTTATGT AGCTCCTATG AAGGCGTTAG CAGCAGAGGT CACGTCAGCA 
TTTAGCCGTC GTCTAGCTCC ATTAAATATG GTTGTGAAGG AGCTTACAGG GGATATGCAA 
CTCACGAAGA CTGAACTTGA AGAAACTCAG ATGATAGTGA CAACACCAGA GAAATGGGAT 
GTCATTACTC GCAAAAGCAG TGACATGTCA ATGTCAATGC TAGTAAAACT ATTGATCATT 
GATGAAGTGC ATCTTCTGAA TGATGATAGA GGTGCAGTGA TAGAGGCGCT AGTTGCTCGG 
ACCCTCCGTC AGGTGGAGTC AACTCAGACA ATGATAAGAA TTGTAGGCCT GTCAGCCACT 
TTACCTAGCT ACCTACAGGT TGCTCAGTTT CTACGAGTGA ATACAGACAC TGGCCTATTC 
TATTTTGATT CAAGTTATCG CCCAGTTCCC TTAGCTCAAC AATACATAGG CATCACTGAG 
CATAACTTTG CTGCTAGAAA CGAATTATTA AATGAGATAT GCTACAAAAA GGTTGTTGAT 
TCAATTAAAC AAGGTCATCA AGCAATGATA TTTGTTCATT CCCGAAAAGA CACTTCAAAA 
ACAGCTGAGA AACTGGTTGA TCTTGCCCGG CAATATGAAA CTCTGGATTT GTTCACGAAT 
GAGACTCATC CTCAATTTCA ACTGATGAAG AAAGACGTTA TGAAGTCCAG AAATAAGGAT 
CTTGTCAAAT TCTTTGAAGC TGGCTTCGGA ATTCATCATG CTGGGATGTT GAGATCTGAT 
AGAACACTTA CGGAGAGGCT GTTTTCTGAT GGTCTCTTGA AGGTGCTTGT TTGCACTGCA 
ACTCTTGCGT GGGGTGTAAA TCTGCCTGCA CACACAGTCG TAATTAAGGG AACACAATTG 
TATGATGCCA AGGCTGGTGG GTGGAAAGAC TTGGGAATGC TTGATGTCAT GCAGATCTTT 
GGAAGGGCAG GGAGACCACA ATTTGATAAG AGTGGTGAGG GAATTATCAT CACATCCCAT 
GACAAGCTGG CATACTATCT CCGGCTGCTG ACTAGTCAGC TTCCCATAGA AAGTCAGTTC 
ATAAGCTCCT TGAAAGATAA CTTAAACGCA GAAGTGGTTT TGGGAACTGT AACTAATGTA 
AAGGAAGCCT GTGCTTGGCT TGGATATACA TATTTGTCAA TTCGGATGAA GTTAAATCCT 
TTGGCTTATG GCATTGGATG GGAAGAGATA ATTGCCGATC CTTCTTTATC CTTGAAGCAA 
CGAGCCCTTG TTGCTGATGC TGCCCGTTCA CTGGACAAAG CTAAGATGAT GAGGTTCGAT 
GAGAAAAGTG GAAACTTTTA CTGTACCGAG CTTGGACGGG TTGCAAGTCA CTTCTATATT 
CAATATTCTA GTGTTGAAAC ATACAACGAA ATGTTGAAGC GGCACATGAA TGAAAGCGAG 
ATAATTAATA TGGTTGCCCA TTCTTCTGAA TTTGAAAATA TTGTGGTAAG AGAGGAAGAG 
CAGCATGAAC TTGAGACTCT TGCACGCTCT TGCTGTCCCT TAGAAGTTAA GGGAGGCCCT 
TCTAACAAAC ATGGAAAAAT TTCGATTCTT ATTCAGTTGT ACATATCCCG TGGATCAATT 
GATGCATTTT CTCTGGTCTC TGATGCTTCA TACATTAGTG CTAGTTTAGC ACGTATAATG 
CGTGCTCTGT TTGAAATATG CTTGCGAAAA GGCTGGTGTG AGATGACTTT ATTTATGTTG 
GAATACTGCA AGGCAGTTGA TCGTCAACTA TGGCCTCATC AGCATCCTCT TCGGCAATTC 
GAAAGGGATC TGCCTTCTGA TCGAAGGGAT GACTTAGACC ATTTGTATGA GATGGAGGAA 
AAGGAAATTG GAGCGCTGAT TCGTTATAAT CCTGGGGGAA GGCATCTAGG ATATTTTCCT 
TCAATTCAGT TGGCAGCTAC TGTTAGTCCA ATCACCCGCA CTGTATTGAA GGTGGATTTG 
CTTATAACTC CAAATTTCAT ATGGAAGGAT CGTTTTCATG GTACTGCTCT TCGTTGGTGG 
ATTCTCATTG AGGACACAGA GAATGATTAT ATCTATCACT CAGATCTTTT CACACTAACG 
AAGCGGATGG CTAGAGGGGA ACCACAGAAG CTTTCTTTTA CCGTACCGAT ATTTGAACCA 
CACCCACCAC AATACTATGT TCATGCGGTT TCTGATTCTT GGTTGCATGC AGAGACTTAC 
TTCACAATCT CTTTTCACAA TCTTGCACTA CCAGAGGCGC GGACGTCTCA CACAGAGCTT 
TTAGACTTAA AACCTCTTCC TGTGACTTCT CTTGGGAATA AGTTGTATGA ATCGCTTTAC 
AAATTTTCAC ATTTCAATCC AATACAGACT CAGATTTTTC ATGTGTTATA TCACACAGAC 
AACAATGTTC TTGTAGGAGC TCCAACTGGA AGTGGGAAAA CTATATCTGC TGAACTTGCA 
ATGTTGCGTC TCTTCAGTAC CCAGCCTGAT ATGAAGGTTG TTTACATAGC ACCACTGAAG 
GCTATTGTTC GAGAAAGGAT GAATGATTGG AAAAAGCATC TTGTTGCTCC ACTTGGAAAA 
GAAATGGTTG AAATGACTGG AGATTACACT CCAGATTTAG TAGCTCTCTT GTCCGCGGAC 
ATCATCATAT CTACCCCTGA AAAATGGGAT GGCATTAGTC GTAATTGGCA TACTCGAAGT 
TATGTCAAAA AGGTCGGCCT TGTTATTTTG GATGAGATTC ACTTGCTTGG AGCCGACAGA 
GGACCGATTC TTGAGGTTAT TGTATCTAGG ATGAGATATA TATCATCACA GACAGAGCGC 
TCCGTCCGGT TTGTTGGGCT TTCTACAGCT CTGGCAAATG CAGGTGATTT GGCTGACTGG 
TTGGGTGTTG GGGAGATCGG ACTTTTCAAT TTTAAACCAA GCGTCAGGCC TGTACCAATT 
GAAGTTCATA TTCAGGGTTA TCCTGGAAAG TATTACTGCC CAAGGATGAA TAGCATGAAC 
AAGCCAGCAT ATGCAGCCAT ATGTACTCAT TCACCTACGA AACCTGTTCT TATCTTTGTT 
TCATCCCGTA GGCAGACAAG GCTTACTGCA TTGGATCTTA TTCAGTTTGC TGCCTCCGAC 
GAACATCCGC GGCAATTTCT GAGTGTGTCA GAGGAAGACT TGCAGATGGT CCTCTCACAG 
ATAACTGATC AAAACCTTCG ACATACGTTG CAATTCGGCA TCGGACTGCA TCATGCAGGT 
TTAAACGACC ACGATAGATC TGCAGTCGAA GAGCTTTTCA CGAACAACAA GATTCAGGTT 
TTGGTCTCAA CAAGTACCTT GGCATGGGGA GTGAACCTAC CAGCTCACTT AGTTATTATA 
AAGGGAACAG AATATTTCGA CGGGAAAACC AAAAGATATG TTGACTTCCC TCTTACAGAG 
ATCTTGCAAA TGATGGGACG TGCAGGGCGA CCACAGTTTG ACCAACATGG GAAAGCGGTT 
ATTCTTGTAC ATGAACCTAA AAAAAGCTTT TACAAAAAGT TCTTGTACGA ACCATTCCCC 
GTAGAAAGTA GTTTGAAAGA GAAGCTGCAC GATCACTTCA ATGCGGAAAT CGTCTCTGGA 
ACAATAGGCA ACAAAGAAGA TGCAGTGCAT TATCTTACAT GGACGTATTT GTTTCGTAGA 
CTGATGGCTA ATCCTGCTTA CTATGGGCTA GAAGGTACTC AAGACGAAAC AATCTGTTCC 
TACTTATCAA GATTGGTGCA AACCACATTT GAGGATCTTG AAGACAGTGG TTGCCTCAAA 
GTAAATGAAG ATAGTGTGGA GCCTACGATG CTGGGTACAA TAGCGTCACA GTATTACCTT 
TGCTACATGA CCGTGTCTAT GTTTGGCTCT AATATAGGTC CTGACACATC GCTTGAAGCA 
TTTTTGCATA TCTTGGCTGG TGCTTCTGAG TATGATGAAC TTCCTGTGCG GCACAATGAG 
GAAAATTACA ATAAAACACT GTCAGATAGA GTTCGGTATC CCGTGGATAA TAACCATCTA 
GATGATCCTC ATGTGAAAGC GAATCTGCTC TTCCAGGCAC ATTTTTCTCA ATTGGCACTT 
CCAATCAGTG ATTATAACAC GGATTTAAAG TCAGTACTGG ATCAAAGCAT TCGGATCCTC 
CAGGCCATGA TTGATATCTG CGCAAACAGT GGATGGCTCT CAAGCTCACT GACGTGTATG 
CGCCTTTTGC AAATGGTCAT GCAGGGCATG TGGTCTGATC AAGATTCGTC ATTATGGATG 
ATCCCATGCA TGAATGACCT TCTTCTAGGA TCTCTGACCG CAAGAGGGAT TCACACGCTA 
CATCAGCTAT TAAATCTTCC AAGGGAAACT CTACAAAGTG TAACCGAAAA CTTCCCTGCA 
TCAAGATTAT CTCAGGATCT CCAACGATTC CCACGGATCC AAATGAATGT GAGACTCCAA 
AAGAAGGATT CCGATGGCAA GAAAAAGCCT TCAACGCTGG AAATCAGATT GGAAAAGACA 
TCAAAGAGGA ATAGCTCGAG GGCATTGGCC CCTCGATTCC CAAAGGTGAA AGACGAAGCG 
TGGTGGTTGG TTCTTGGTGA TACTTCAACT TCTGAGCTCT TTGCTGTGAA GAGAGTTTCG 
TTCACTGGTC GTCTGATCAC ACGCATGGAG TTACCTCCCA ATATAACCTC TTTTCAGGAT 
ACGAAACTCA TCTTAGTTTC GGATTGTTAT TTGGGGTTTG AACAAGAACA CTCCATTGAA 
CAACTTGCAC GACGCGGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT5G61140 ( NM_001203655.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190584.1 Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (AT5G61140), mRNA.

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CloneID	OAb29763
Clone ID Related Accession (Same CDS sequence)	NM_001203655.2
Accession Version	NM_001203655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	U5 small nuclear ribonucleoprotein helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001203655.1.

ATGGTCCACA ACTCCCCATG TAATAAACAT AAAACGACGT CGATTTCGTG GACCCGTATC 
TTAGCGGGTC GGTGTACGGG CCTATTTGTT CAAGTATCTA ACCGACGCCG TTTTAGAACG 
CAAGCGATCG CCTTCTTCCT CGGAGAGCCG ACCAGAGGTG AAAACACCAC AGAGGAGAAG 
AATCCTAAAC GACACAAGTA CAGGGATTTT GTCATGTTAG TTCAGCTTCC TCGCTTGACA 
AGCTCTCTTA GAGAGCCTTT CGACATCGAT CAGGCTTACC TTCGCCGCAA AACTATACTC 
CAAACTCTTA ATAAGCCTCG CAGCTCCGGT AATCGCCTTG ATGAATCTGA CCTTGCTAAG 
AGGATAGTTC ATCAATGGGA AGGAGCTTCT CTCGAAGTGC GACAAGCATA CAAGCAGTTT 
ATTGGAGCTG TAGTGGAATT GATTGATCGG GAAGTTCCAT CAGATGAGTT TCGCGAGGTT 
GCTTTTTCCG CTTATCGTCT GTTTAACAAT CCAGTGGAGG AAGATGATAG TGATATCAAT 
GATAATATAA GCATTTCCGG GAAGAAGTTA GAGTTGCAAA ATCTTGTCGG GCATGCTGTC 
TCAGATGCTA ATGTAAAAAA CGTGGCCTCC TTTGCTCAGG CACTTTACTC AATACAGCCT 
ACTCATCAGT CTGAGACATA TGCTGATGAA GTTAATGGGG GAGCTGAGTT TGGTGCCGAC 
CTTGTTTTCA ATCTACCAGC TCGTTTTTTG GTGGAAGCAT CTCTTGACGA AACTGGGTTT 
GTGGATGTAG AAAGCAATGA TGCTCATACA TCGTTTTCTG AAGGATGGAG TGGTGTTAGC 
GACACGAAAA ATAATCTCAG TGCAGGAAAA TTCAATTTAA GTTGGCTAAG AGATGCATGT 
GGTCGGATGG TTAGAGAAAC CAATTCACAA CTTTCCCGTG AAGAATTGGC TATGGCTATA 
TGCAGATTTC TGGATTCGGA TAAACCCGGT GAGGAGATAG CTGGGGATCT GCTGGATCTT 
GTTGGGGATG GTGCATTTGA GACTGTTCAA GACCTTATTA TGCATCGGAA AGAAATTGTT 
GATGCTATCC ATCATGGCCA AATGATTCTC AAGTCTGACA AAGCAGCTTC AAACACCCAA 
TCTCGGATGC CTACCTATGG AACACAGGTT ACTGTTCAAA CAGAGTCTGC AAAGCAAATT 
GAAAAACTTC GGCGTAAGGA GGAAAAGAAA AATAAACGAA ATGCTGATCT TGGTTTAGAG 
AGTGAAATAT CAGAGGCCAA TTTTTCTTCT TTACTTGAGG CAAGTGAAAA GAAGACTGCA 
TTTGAGGATC TGATTGGGTC TGGAGAAGCG AATTCTCTTG CACTTGCCCT TCCCCAAGGT 
ACTGTAAGGA AGCACTTAAA AGGATATGAG GAAGTGTTTA TCCCGCCAAC CCCTACTGCA 
CAAATGAAAC CTGGCGAGAA GCTGATTGAG ATAAAAGAGT TAGATGACTT TGCTCAAGCT 
GCTTTTCATG GTTACAAGTC TTTAAACCGT ATTCAGAGTC GCATATTTCA GACAGTTTAC 
CACACCAATG AGAATATATT GGTCTGTGCT CCAACAGGAG CTGGTAAAAC AAATATAGCT 
ATGATTTCTG TTCTACATGA GATCAAACAA CACTTCAGGG ATGGCTACTT GCACAAAAAT 
GAGTTTAAGA TTGTTTATGT AGCTCCTATG AAGGCGTTAG CAGCAGAGGT CACGTCAGCA 
TTTAGCCGTC GTCTAGCTCC ATTAAATATG GTTGTGAAGG AGCTTACAGG GGATATGCAA 
CTCACGAAGA CTGAACTTGA AGAAACTCAG ATGATAGTGA CAACACCAGA GAAATGGGAT 
GTCATTACTC GCAAAAGCAG TGACATGTCA ATGTCAATGC TAGTAAAACT ATTGATCATT 
GATGAAGTGC ATCTTCTGAA TGATGATAGA GGTGCAGTGA TAGAGGCGCT AGTTGCTCGG 
ACCCTCCGTC AGGTGGAGTC AACTCAGACA ATGATAAGAA TTGTAGGCCT GTCAGCCACT 
TTACCTAGCT ACCTACAGGT TGCTCAGTTT CTACGAGTGA ATACAGACAC TGGCCTATTC 
TATTTTGATT CAAGTTATCG CCCAGTTCCC TTAGCTCAAC AATACATAGG CATCACTGAG 
CATAACTTTG CTGCTAGAAA CGAATTATTA AATGAGATAT GCTACAAAAA GGTTGTTGAT 
TCAATTAAAC AAGGTCATCA AGCAATGATA TTTGTTCATT CCCGAAAAGA CACTTCAAAA 
ACAGCTGAGA AACTGGTTGA TCTTGCCCGG CAATATGAAA CTCTGGATTT GTTCACGAAT 
GAGACTCATC CTCAATTTCA ACTGATGAAG AAAGACGTTA TGAAGTCCAG AAATAAGGAT 
CTTGTCAAAT TCTTTGAAGC TGGCTTCGGA ATTCATCATG CTGGGATGTT GAGATCTGAT 
AGAACACTTA CGGAGAGGCT GTTTTCTGAT GGTCTCTTGA AGGTGCTTGT TTGCACTGCA 
ACTCTTGCGT GGGGTGTAAA TCTGCCTGCA CACACAGTCG TAATTAAGGG AACACAATTG 
TATGATGCCA AGGCTGGTGG GTGGAAAGAC TTGGGAATGC TTGATGTCAT GCAGATCTTT 
GGAAGGGCAG GGAGACCACA ATTTGATAAG AGTGGTGAGG GAATTATCAT CACATCCCAT 
GACAAGCTGG CATACTATCT CCGGCTGCTG ACTAGTCAGC TTCCCATAGA AAGTCAGTTC 
ATAAGCTCCT TGAAAGATAA CTTAAACGCA GAAGTGGTTT TGGGAACTGT AACTAATGTA 
AAGGAAGCCT GTGCTTGGCT TGGATATACA TATTTGTCAA TTCGGATGAA GTTAAATCCT 
TTGGCTTATG GCATTGGATG GGAAGAGATA ATTGCCGATC CTTCTTTATC CTTGAAGCAA 
CGAGCCCTTG TTGCTGATGC TGCCCGTTCA CTGGACAAAG CTAAGATGAT GAGGTTCGAT 
GAGAAAAGTG GAAACTTTTA CTGTACCGAG CTTGGACGGG TTGCAAGTCA CTTCTATATT 
CAATATTCTA GTGTTGAAAC ATACAACGAA ATGTTGAAGC GGCACATGAA TGAAAGCGAG 
ATAATTAATA TGGTTGCCCA TTCTTCTGAA TTTGAAAATA TTGTGGTAAG AGAGGAAGAG 
CAGCATGAAC TTGAGACTCT TGCACGCTCT TGCTGTCCCT TAGAAGTTAA GGGAGGCCCT 
TCTAACAAAC ATGGAAAAAT TTCGATTCTT ATTCAGTTGT ACATATCCCG TGGATCAATT 
GATGCATTTT CTCTGGTCTC TGATGCTTCA TACATTAGTG CTAGTTTAGC ACGTATAATG 
CGTGCTCTGT TTGAAATATG CTTGCGAAAA GGCTGGTGTG AGATGACTTT ATTTATGTTG 
GAATACTGCA AGGCAGTTGA TCGTCAACTA TGGCCTCATC AGCATCCTCT TCGGCAATTC 
GAAAGGGATC TGCCTTCTGA TATATTAAGA AAACTTGAAG AGCGAAGGGA TGACTTAGAC 
CATTTGTATG AGATGGAGGA AAAGGAAATT GGAGCGCTGA TTCGTTATAA TCCTGGGGGA 
AGGTTGGTCA AGCAGCATCT AGGATATTTT CCTTCAATTC AGTTGGCAGC TACTGTTAGT 
CCAATCACCC GCACTGTATT GAAGGTGGAT TTGCTTATAA CTCCAAATTT CATATGGAAG 
GATCGTTTTC ATGGTACTGC TCTTCGTTGG TGGATTCTCA TTGAGGACAC AGAGAATGAT 
TATATCTATC ACTCAGATCT TTTCACACTA ACGAAGCGGA TGGCTAGAGG GGAACCACAG 
AAGCTTTCTT TTACCGTACC GATATTTGAA CCACACCCAC CACAATACTA TGTTCATGCG 
GTTTCTGATT CTTGGTTGCA TGCAGAGACT TACTTCACAA TCTCTTTTCA CAATCTTGCA 
CTACCAGAGG CGCGGACGTC TCACACAGAG CTTTTAGACT TAAAACCTCT TCCTGTGACT 
TCTCTTGGGA ATAAGTTGTA TGAATCGCTT TACAAATTTT CACATTTCAA TCCAATACAG 
ACTCAGATTT TTCATGTGTT ATATCACACA GACAACAATG TTCTTGTAGG AGCTCCAACT 
GGAAGTGGGA AAACTATATC TGCTGAACTT GCAATGTTGC GTCTCTTCAG TACCCAGCCT 
GATATGAAGG TTGTTTACAT AGCACCACTG AAGGCTATTG TTCGAGAAAG GATGAATGAT 
TGGAAAAAGC ATCTTGTTGC TCCACTTGGA AAAGAAATGG TTGAAATGAC TGGAGATTAC 
ACTCCAGATT TAGTAGCTCT CTTGTCCGCG GACATCATCA TATCTACCCC TGAAAAATGG 
GATGGCATTA GTCGTAATTG GCATACTCGA AGTTATGTCA AAAAGGTCGG CCTTGTTATT 
TTGGATGAGA TTCACTTGCT TGGAGCCGAC AGAGGACCGA TTCTTGAGGT TATTGTATCT 
AGGATGAGAT ATATATCATC ACAGACAGAG CGCTCCGTCC GGTTTGTTGG GCTTTCTACA 
GCTCTGGCAA ATGCAGGTGA TTTGGCTGAC TGGTTGGGTG TTGGGGAGAT CGGACTTTTC 
AATTTTAAAC CAAGCGTCAG GCCTGTACCA ATTGAAGTTC ATATTCAGGG TTATCCTGGA 
AAGTATTACT GCCCAAGGAT GAATAGCATG AACAAGCCAG CATATGCAGC CATATGTACT 
CATTCACCTA CGAAACCTGT TCTTATCTTT GTTTCATCCC GTAGGCAGAC AAGGCTTACT 
GCATTGGATC TTATTCAGTT TGCTGCCTCC GACGAACATC CGCGGCAATT TCTGAGTGTG 
TCAGAGGAAG ACTTGCAGAT GGTCCTCTCA CAGATAACTG ATCAAAACCT TCGACATACG 
TTGCAATTCG GCATCGGACT GCATCATGCA GGTTTAAACG ACCACGATAG ATCTGCAGTC 
GAAGAGCTTT TCACGAACAA CAAGATTCAG GTTTTGGTCT CAACAAGTAC CTTGGCATGG 
GGAGTGAACC TACCAGCTCA CTTAGTTATT ATAAAGGGAA CAGAATATTT CGACGGGAAA 
ACCAAAAGAT ATGTTGACTT CCCTCTTACA GAGATCTTGC AAATGATGGG ACGTGCAGGG 
CGACCACAGT TTGACCAACA TGGGAAAGCG GTTATTCTTG TACATGAACC TAAAAAAAGC 
TTTTACAAAA AGTTCTTGTA CGAACCATTC CCCGTAGAAA GTAGTTTGAA AGAGAAGCTG 
CACGATCACT TCAATGCGGA AATCGTCTCT GGAACAATAG GCAACAAAGA AGATGCAGTG 
CATTATCTTA CATGGACGTA TTTGTTTCGT AGACTGATGG CTAATCCTGC TTACTATGGG 
CTAGAAGGTA CTCAAGACGA AACAATCTGT TCCTACTTAT CAAGATTGGT GCAAACCACA 
TTTGAGGATC TTGAAGACAG TGGTTGCCTC AAAGTAAATG AAGATAGTGT GGAGCCTACG 
ATGCTGGGTA CAATAGCGTC ACAGTATTAC CTTTGCTACA TGACCGTGTC TATGTTTGGC 
TCTAATATAG GTCCTGACAC ATCGCTTGAA GCATTTTTGC ATATCTTGGC TGGTGCTTCT 
GAGTATGATG AACTTCCTGT GCGGCACAAT GAGGAAAATT ACAATAAAAC ACTGTCAGAT 
AGAGTTCGGT ATCCCGTGGA TAATAACCAT CTAGATGATC CTCATGTGAA AGCGAATCTG 
CTCTTCCAGG CACATTTTTC TCAATTGGCA CTTCCAATCA GTGATTATAA CACGGATTTA 
AAGTCAGTAC TGGATCAAAG CATTCGGATC CTCCAGGCCA TGATTGATAT CTGCGCAAAC 
AGTGGATGGC TCTCAAGCTC ACTGACGTGT ATGCGCCTTT TGCAAATGGT CATGCAGGGC 
ATGTGGTCTG ATCAAGATTC GTCATTATGG ATGATCCCAT GCATGAATGA CCTTCTTCTA 
GGATCTCTGA CCGCAAGAGG GATTCACACG CTACATCAGC TATTAAATCT TCCAAGGGAA 
ACTCTACAAA GTGTAACCGA AAACTTCCCT GCATCAAGAT TATCTCAGGA TCTCCAACGA 
TTCCCACGGA TCCAAATGAA TGTGAGACTC CAAAAGAAGG ATTCCGATGG CAAGAAAAAG 
CCTTCAACGC TGGAAATCAG ATTGGAAAAG ACATCAAAGA GGAATAGCTC GAGGGCATTG 
GCCCCTCGAT TCCCAAAGGT GAAAGACGAA GCGTGGTGGT TGGTTCTTGG TGATACTTCA 
ACTTCTGAGC TCTTTGCTGT GAAGAGAGTT TCGTTCACTG GTCGTCTGAT CACACGCATG 
GAGTTACCTC CCAATATAAC CTCTTTTCAG GATACGAAAC TCATCTTAGT TTCGGATTGT 
TATTTGGGGT TTGAACAAGA ACACTCCATT GAACAACTTG CACGACGCGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190584.1
CDS	94..6567
Translation	MVHNSPCNKHKTTSISWTRILAGRCTGLFVQVSNRRRFRTQAIAFFLGEPTRGENTTEEKNPKRHKYRDFVMLVQLPRLTSSLREPFDIDQAYLRRKTILQTLNKPRSSGNRLDESDLAKRIVHQWEGASLEVRQAYKQFIGAVVELIDREVPSDEFREVAFSAYRLFNNPVEEDDSDINDNISISGKKLELQNLVGHAVSDANVKNVASFAQALYSIQPTHQSETYADEVNGGAEFGADLVFNLPARFLVEASLDETGFVDVESNDAHTSFSEGWSGVSDTKNNLSAGKFNLSWLRDACGRMVRETNSQLSREELAMAICRFLDSDKPGEEIAGDLLDLVGDGAFETVQDLIMHRKEIVDAIHHGQMILKSDKAASNTQSRMPTYGTQVTVQTESAKQIEKLRRKEEKKNKRNADLGLESEISEANFSSLLEASEKKTAFEDLIGSGEANSLALALPQGTVRKHLKGYEEVFIPPTPTAQMKPGEKLIEIKELDDFAQAAFHGYKSLNRIQSRIFQTVYHTNENILVCAPTGAGKTNIAMISVLHEIKQHFRDGYLHKNEFKIVYVAPMKALAAEVTSAFSRRLAPLNMVVKELTGDMQLTKTELEETQMIVTTPEKWDVITRKSSDMSMSMLVKLLIIDEVHLLNDDRGAVIEALVARTLRQVESTQTMIRIVGLSATLPSYLQVAQFLRVNTDTGLFYFDSSYRPVPLAQQYIGITEHNFAARNELLNEICYKKVVDSIKQGHQAMIFVHSRKDTSKTAEKLVDLARQYETLDLFTNETHPQFQLMKKDVMKSRNKDLVKFFEAGFGIHHAGMLRSDRTLTERLFSDGLLKVLVCTATLAWGVNLPAHTVVIKGTQLYDAKAGGWKDLGMLDVMQIFGRAGRPQFDKSGEGIIITSHDKLAYYLRLLTSQLPIESQFISSLKDNLNAEVVLGTVTNVKEACAWLGYTYLSIRMKLNPLAYGIGWEEIIADPSLSLKQRALVADAARSLDKAKMMRFDEKSGNFYCTELGRVASHFYIQYSSVETYNEMLKRHMNESEIINMVAHSSEFENIVVREEEQHELETLARSCCPLEVKGGPSNKHGKISILIQLYISRGSIDAFSLVSDASYISASLARIMRALFEICLRKGWCEMTLFMLEYCKAVDRQLWPHQHPLRQFERDLPSDILRKLEERRDDLDHLYEMEEKEIGALIRYNPGGRLVKQHLGYFPSIQLAATVSPITRTVLKVDLLITPNFIWKDRFHGTALRWWILIEDTENDYIYHSDLFTLTKRMARGEPQKLSFTVPIFEPHPPQYYVHAVSDSWLHAETYFTISFHNLALPEARTSHTELLDLKPLPVTSLGNKLYESLYKFSHFNPIQTQIFHVLYHTDNNVLVGAPTGSGKTISAELAMLRLFSTQPDMKVVYIAPLKAIVRERMNDWKKHLVAPLGKEMVEMTGDYTPDLVALLSADIIISTPEKWDGISRNWHTRSYVKKVGLVILDEIHLLGADRGPILEVIVSRMRYISSQTERSVRFVGLSTALANAGDLADWLGVGEIGLFNFKPSVRPVPIEVHIQGYPGKYYCPRMNSMNKPAYAAICTHSPTKPVLIFVSSRRQTRLTALDLIQFAASDEHPRQFLSVSEEDLQMVLSQITDQNLRHTLQFGIGLHHAGLNDHDRSAVEELFTNNKIQVLVSTSTLAWGVNLPAHLVIIKGTEYFDGKTKRYVDFPLTEILQMMGRAGRPQFDQHGKAVILVHEPKKSFYKKFLYEPFPVESSLKEKLHDHFNAEIVSGTIGNKEDAVHYLTWTYLFRRLMANPAYYGLEGTQDETICSYLSRLVQTTFEDLEDSGCLKVNEDSVEPTMLGTIASQYYLCYMTVSMFGSNIGPDTSLEAFLHILAGASEYDELPVRHNEENYNKTLSDRVRYPVDNNHLDDPHVKANLLFQAHFSQLALPISDYNTDLKSVLDQSIRILQAMIDICANSGWLSSSLTCMRLLQMVMQGMWSDQDSSLWMIPCMNDLLLGSLTARGIHTLHQLLNLPRETLQSVTENFPASRLSQDLQRFPRIQMNVRLQKKDSDGKKKPSTLEIRLEKTSKRNSSRALAPRFPKVKDEAWWLVLGDTSTSELFAVKRVSFTGRLITRMELPPNITSFQDTKLILVSDCYLGFEQEHSIEQLARRG

Target ORF information:

RefSeq Version	NM_001203655.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana U5 small nuclear ribonucleoprotein helicase (AT5G61140), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203655.2

ORF Insert Sequence:

ATGGTCCACA ACTCCCCATG TAATAAACAT AAAACGACGT CGATTTCGTG GACCCGTATC 
TTAGCGGGTC GGTGTACGGG CCTATTTGTT CAAGTATCTA ACCGACGCCG TTTTAGAACG 
CAAGCGATCG CCTTCTTCCT CGGAGAGCCG ACCAGAGGTG AAAACACCAC AGAGGAGAAG 
AATCCTAAAC GACACAAGTA CAGGGATTTT GTCATGTTAG TTCAGCTTCC TCGCTTGACA 
AGCTCTCTTA GAGAGCCTTT CGACATCGAT CAGGCTTACC TTCGCCGCAA AACTATACTC 
CAAACTCTTA ATAAGCCTCG CAGCTCCGGT AATCGCCTTG ATGAATCTGA CCTTGCTAAG 
AGGATAGTTC ATCAATGGGA AGGAGCTTCT CTCGAAGTGC GACAAGCATA CAAGCAGTTT 
ATTGGAGCTG TAGTGGAATT GATTGATCGG GAAGTTCCAT CAGATGAGTT TCGCGAGGTT 
GCTTTTTCCG CTTATCGTCT GTTTAACAAT CCAGTGGAGG AAGATGATAG TGATATCAAT 
GATAATATAA GCATTTCCGG GAAGAAGTTA GAGTTGCAAA ATCTTGTCGG GCATGCTGTC 
TCAGATGCTA ATGTAAAAAA CGTGGCCTCC TTTGCTCAGG CACTTTACTC AATACAGCCT 
ACTCATCAGT CTGAGACATA TGCTGATGAA GTTAATGGGG GAGCTGAGTT TGGTGCCGAC 
CTTGTTTTCA ATCTACCAGC TCGTTTTTTG GTGGAAGCAT CTCTTGACGA AACTGGGTTT 
GTGGATGTAG AAAGCAATGA TGCTCATACA TCGTTTTCTG AAGGATGGAG TGGTGTTAGC 
GACACGAAAA ATAATCTCAG TGCAGGAAAA TTCAATTTAA GTTGGCTAAG AGATGCATGT 
GGTCGGATGG TTAGAGAAAC CAATTCACAA CTTTCCCGTG AAGAATTGGC TATGGCTATA 
TGCAGATTTC TGGATTCGGA TAAACCCGGT GAGGAGATAG CTGGGGATCT GCTGGATCTT 
GTTGGGGATG GTGCATTTGA GACTGTTCAA GACCTTATTA TGCATCGGAA AGAAATTGTT 
GATGCTATCC ATCATGGCCA AATGATTCTC AAGTCTGACA AAGCAGCTTC AAACACCCAA 
TCTCGGATGC CTACCTATGG AACACAGGTT ACTGTTCAAA CAGAGTCTGC AAAGCAAATT 
GAAAAACTTC GGCGTAAGGA GGAAAAGAAA AATAAACGAA ATGCTGATCT TGGTTTAGAG 
AGTGAAATAT CAGAGGCCAA TTTTTCTTCT TTACTTGAGG CAAGTGAAAA GAAGACTGCA 
TTTGAGGATC TGATTGGGTC TGGAGAAGCG AATTCTCTTG CACTTGCCCT TCCCCAAGGT 
ACTGTAAGGA AGCACTTAAA AGGATATGAG GAAGTGTTTA TCCCGCCAAC CCCTACTGCA 
CAAATGAAAC CTGGCGAGAA GCTGATTGAG ATAAAAGAGT TAGATGACTT TGCTCAAGCT 
GCTTTTCATG GTTACAAGTC TTTAAACCGT ATTCAGAGTC GCATATTTCA GACAGTTTAC 
CACACCAATG AGAATATATT GGTCTGTGCT CCAACAGGAG CTGGTAAAAC AAATATAGCT 
ATGATTTCTG TTCTACATGA GATCAAACAA CACTTCAGGG ATGGCTACTT GCACAAAAAT 
GAGTTTAAGA TTGTTTATGT AGCTCCTATG AAGGCGTTAG CAGCAGAGGT CACGTCAGCA 
TTTAGCCGTC GTCTAGCTCC ATTAAATATG GTTGTGAAGG AGCTTACAGG GGATATGCAA 
CTCACGAAGA CTGAACTTGA AGAAACTCAG ATGATAGTGA CAACACCAGA GAAATGGGAT 
GTCATTACTC GCAAAAGCAG TGACATGTCA ATGTCAATGC TAGTAAAACT ATTGATCATT 
GATGAAGTGC ATCTTCTGAA TGATGATAGA GGTGCAGTGA TAGAGGCGCT AGTTGCTCGG 
ACCCTCCGTC AGGTGGAGTC AACTCAGACA ATGATAAGAA TTGTAGGCCT GTCAGCCACT 
TTACCTAGCT ACCTACAGGT TGCTCAGTTT CTACGAGTGA ATACAGACAC TGGCCTATTC 
TATTTTGATT CAAGTTATCG CCCAGTTCCC TTAGCTCAAC AATACATAGG CATCACTGAG 
CATAACTTTG CTGCTAGAAA CGAATTATTA AATGAGATAT GCTACAAAAA GGTTGTTGAT 
TCAATTAAAC AAGGTCATCA AGCAATGATA TTTGTTCATT CCCGAAAAGA CACTTCAAAA 
ACAGCTGAGA AACTGGTTGA TCTTGCCCGG CAATATGAAA CTCTGGATTT GTTCACGAAT 
GAGACTCATC CTCAATTTCA ACTGATGAAG AAAGACGTTA TGAAGTCCAG AAATAAGGAT 
CTTGTCAAAT TCTTTGAAGC TGGCTTCGGA ATTCATCATG CTGGGATGTT GAGATCTGAT 
AGAACACTTA CGGAGAGGCT GTTTTCTGAT GGTCTCTTGA AGGTGCTTGT TTGCACTGCA 
ACTCTTGCGT GGGGTGTAAA TCTGCCTGCA CACACAGTCG TAATTAAGGG AACACAATTG 
TATGATGCCA AGGCTGGTGG GTGGAAAGAC TTGGGAATGC TTGATGTCAT GCAGATCTTT 
GGAAGGGCAG GGAGACCACA ATTTGATAAG AGTGGTGAGG GAATTATCAT CACATCCCAT 
GACAAGCTGG CATACTATCT CCGGCTGCTG ACTAGTCAGC TTCCCATAGA AAGTCAGTTC 
ATAAGCTCCT TGAAAGATAA CTTAAACGCA GAAGTGGTTT TGGGAACTGT AACTAATGTA 
AAGGAAGCCT GTGCTTGGCT TGGATATACA TATTTGTCAA TTCGGATGAA GTTAAATCCT 
TTGGCTTATG GCATTGGATG GGAAGAGATA ATTGCCGATC CTTCTTTATC CTTGAAGCAA 
CGAGCCCTTG TTGCTGATGC TGCCCGTTCA CTGGACAAAG CTAAGATGAT GAGGTTCGAT 
GAGAAAAGTG GAAACTTTTA CTGTACCGAG CTTGGACGGG TTGCAAGTCA CTTCTATATT 
CAATATTCTA GTGTTGAAAC ATACAACGAA ATGTTGAAGC GGCACATGAA TGAAAGCGAG 
ATAATTAATA TGGTTGCCCA TTCTTCTGAA TTTGAAAATA TTGTGGTAAG AGAGGAAGAG 
CAGCATGAAC TTGAGACTCT TGCACGCTCT TGCTGTCCCT TAGAAGTTAA GGGAGGCCCT 
TCTAACAAAC ATGGAAAAAT TTCGATTCTT ATTCAGTTGT ACATATCCCG TGGATCAATT 
GATGCATTTT CTCTGGTCTC TGATGCTTCA TACATTAGTG CTAGTTTAGC ACGTATAATG 
CGTGCTCTGT TTGAAATATG CTTGCGAAAA GGCTGGTGTG AGATGACTTT ATTTATGTTG 
GAATACTGCA AGGCAGTTGA TCGTCAACTA TGGCCTCATC AGCATCCTCT TCGGCAATTC 
GAAAGGGATC TGCCTTCTGA TATATTAAGA AAACTTGAAG AGCGAAGGGA TGACTTAGAC 
CATTTGTATG AGATGGAGGA AAAGGAAATT GGAGCGCTGA TTCGTTATAA TCCTGGGGGA 
AGGTTGGTCA AGCAGCATCT AGGATATTTT CCTTCAATTC AGTTGGCAGC TACTGTTAGT 
CCAATCACCC GCACTGTATT GAAGGTGGAT TTGCTTATAA CTCCAAATTT CATATGGAAG 
GATCGTTTTC ATGGTACTGC TCTTCGTTGG TGGATTCTCA TTGAGGACAC AGAGAATGAT 
TATATCTATC ACTCAGATCT TTTCACACTA ACGAAGCGGA TGGCTAGAGG GGAACCACAG 
AAGCTTTCTT TTACCGTACC GATATTTGAA CCACACCCAC CACAATACTA TGTTCATGCG 
GTTTCTGATT CTTGGTTGCA TGCAGAGACT TACTTCACAA TCTCTTTTCA CAATCTTGCA 
CTACCAGAGG CGCGGACGTC TCACACAGAG CTTTTAGACT TAAAACCTCT TCCTGTGACT 
TCTCTTGGGA ATAAGTTGTA TGAATCGCTT TACAAATTTT CACATTTCAA TCCAATACAG 
ACTCAGATTT TTCATGTGTT ATATCACACA GACAACAATG TTCTTGTAGG AGCTCCAACT 
GGAAGTGGGA AAACTATATC TGCTGAACTT GCAATGTTGC GTCTCTTCAG TACCCAGCCT 
GATATGAAGG TTGTTTACAT AGCACCACTG AAGGCTATTG TTCGAGAAAG GATGAATGAT 
TGGAAAAAGC ATCTTGTTGC TCCACTTGGA AAAGAAATGG TTGAAATGAC TGGAGATTAC 
ACTCCAGATT TAGTAGCTCT CTTGTCCGCG GACATCATCA TATCTACCCC TGAAAAATGG 
GATGGCATTA GTCGTAATTG GCATACTCGA AGTTATGTCA AAAAGGTCGG CCTTGTTATT 
TTGGATGAGA TTCACTTGCT TGGAGCCGAC AGAGGACCGA TTCTTGAGGT TATTGTATCT 
AGGATGAGAT ATATATCATC ACAGACAGAG CGCTCCGTCC GGTTTGTTGG GCTTTCTACA 
GCTCTGGCAA ATGCAGGTGA TTTGGCTGAC TGGTTGGGTG TTGGGGAGAT CGGACTTTTC 
AATTTTAAAC CAAGCGTCAG GCCTGTACCA ATTGAAGTTC ATATTCAGGG TTATCCTGGA 
AAGTATTACT GCCCAAGGAT GAATAGCATG AACAAGCCAG CATATGCAGC CATATGTACT 
CATTCACCTA CGAAACCTGT TCTTATCTTT GTTTCATCCC GTAGGCAGAC AAGGCTTACT 
GCATTGGATC TTATTCAGTT TGCTGCCTCC GACGAACATC CGCGGCAATT TCTGAGTGTG 
TCAGAGGAAG ACTTGCAGAT GGTCCTCTCA CAGATAACTG ATCAAAACCT TCGACATACG 
TTGCAATTCG GCATCGGACT GCATCATGCA GGTTTAAACG ACCACGATAG ATCTGCAGTC 
GAAGAGCTTT TCACGAACAA CAAGATTCAG GTTTTGGTCT CAACAAGTAC CTTGGCATGG 
GGAGTGAACC TACCAGCTCA CTTAGTTATT ATAAAGGGAA CAGAATATTT CGACGGGAAA 
ACCAAAAGAT ATGTTGACTT CCCTCTTACA GAGATCTTGC AAATGATGGG ACGTGCAGGG 
CGACCACAGT TTGACCAACA TGGGAAAGCG GTTATTCTTG TACATGAACC TAAAAAAAGC 
TTTTACAAAA AGTTCTTGTA CGAACCATTC CCCGTAGAAA GTAGTTTGAA AGAGAAGCTG 
CACGATCACT TCAATGCGGA AATCGTCTCT GGAACAATAG GCAACAAAGA AGATGCAGTG 
CATTATCTTA CATGGACGTA TTTGTTTCGT AGACTGATGG CTAATCCTGC TTACTATGGG 
CTAGAAGGTA CTCAAGACGA AACAATCTGT TCCTACTTAT CAAGATTGGT GCAAACCACA 
TTTGAGGATC TTGAAGACAG TGGTTGCCTC AAAGTAAATG AAGATAGTGT GGAGCCTACG 
ATGCTGGGTA CAATAGCGTC ACAGTATTAC CTTTGCTACA TGACCGTGTC TATGTTTGGC 
TCTAATATAG GTCCTGACAC ATCGCTTGAA GCATTTTTGC ATATCTTGGC TGGTGCTTCT 
GAGTATGATG AACTTCCTGT GCGGCACAAT GAGGAAAATT ACAATAAAAC ACTGTCAGAT 
AGAGTTCGGT ATCCCGTGGA TAATAACCAT CTAGATGATC CTCATGTGAA AGCGAATCTG 
CTCTTCCAGG CACATTTTTC TCAATTGGCA CTTCCAATCA GTGATTATAA CACGGATTTA 
AAGTCAGTAC TGGATCAAAG CATTCGGATC CTCCAGGCCA TGATTGATAT CTGCGCAAAC 
AGTGGATGGC TCTCAAGCTC ACTGACGTGT ATGCGCCTTT TGCAAATGGT CATGCAGGGC 
ATGTGGTCTG ATCAAGATTC GTCATTATGG ATGATCCCAT GCATGAATGA CCTTCTTCTA 
GGATCTCTGA CCGCAAGAGG GATTCACACG CTACATCAGC TATTAAATCT TCCAAGGGAA 
ACTCTACAAA GTGTAACCGA AAACTTCCCT GCATCAAGAT TATCTCAGGA TCTCCAACGA 
TTCCCACGGA TCCAAATGAA TGTGAGACTC CAAAAGAAGG ATTCCGATGG CAAGAAAAAG 
CCTTCAACGC TGGAAATCAG ATTGGAAAAG ACATCAAAGA GGAATAGCTC GAGGGCATTG 
GCCCCTCGAT TCCCAAAGGT GAAAGACGAA GCGTGGTGGT TGGTTCTTGG TGATACTTCA 
ACTTCTGAGC TCTTTGCTGT GAAGAGAGTT TCGTTCACTG GTCGTCTGAT CACACGCATG 
GAGTTACCTC CCAATATAAC CTCTTTTCAG GATACGAAAC TCATCTTAGT TTCGGATTGT 
TATTTGGGGT TTGAACAAGA ACACTCCATT GAACAACTTG CACGACGCGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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AT5G61140 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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