MSI1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MSI1
Entrez Gene ID	835935
Full Name	Transducin/WD40 repeat-like superfamily protein
Synonyms	ARABIDOPSIS MULTICOPY SUPRESSOR OF IRA1,ATMSI1,MATERNAL EFFECT EMBRYO ARREST 70,MCK7.10,MCK7_10,MEE70,MULTICOPY SUPRESSOR OF IRA1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_125208.3	NP_200631.1	Transducin/WD40 repeat-like superfamily protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rbbp4	NP_033056.2
Gallus gallus (chicken)	RBBP4	NP_990183.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003228	XP_312936.3
Homo sapiens (human)	RBBP4	NP_005601.1
Bos taurus (cattle)	RBBP4	NP_001070481.1
Danio rerio (zebrafish)	rbb4	NP_997760.1
Schizosaccharomyces pombe (fission yeast)	mis16	NP_587881.1
Neurospora crassa	NCU06679	XP_960994.2
Arabidopsis thaliana (thale cress)	MSI1	NP_200631.1
Pan troglodytes (chimpanzee)	RBBP4	NP_001233303.1
Drosophila melanogaster (fruit fly)	Caf1	NP_001163617.1
Magnaporthe oryzae (rice blast fungus)	MGG_07323	XP_003715557.1
Oryza sativa (rice)	Os03g0640100	NP_001050741.1
Canis lupus familiaris (dog)	RBBP4	XP_864445.1
Rattus norvegicus (Norway rat)	Rbbp4	NP_001101382.1
Xenopus tropicalis (tropical clawed frog)	rbbp4	NP_001011394.2
	RBBP4	XP_001104493.1
Caenorhabditis elegans (roundworm)	lin-53	NP_492552.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results reveal the presence of an MSI1-HDA19 complex that fine-tunes abscissic acid signaling in Arabidopsis.
Title: The WD40 Domain Protein MSI1 Functions in a Histone Deacetylase Complex to Fine-Tune Abscisic Acid Signaling.

MSI1 serves to link PRC2 to LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), a protein that binds H3K27me3 in vitro and in vivo and is required for a functional polycomb group system.
Title: Arabidopsis MSI1 connects LHP1 to PRC2 complexes.

CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting.
Title: The Arabidopsis CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting.

Data report that MSI1 can bind to the chromatin of the drought-inducible downstream target RD20 and suggest a new role for MSI1 in the negative regulation of the Arabidopsis drought-stress response.
Title: Arabidopsis MSI1 is required for negative regulation of the response to drought stress.

Rb-dependent activation is mediated by direct transcriptional repression of MET1 during female gametogenesis.
Title: Retinoblastoma and its binding partner MSI1 control imprinting in Arabidopsis.

The following MSI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb21766	NM_125208.3 Latest version!	Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (MSI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSI1 ( NM_125208.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200631.1 Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (MSI1), mRNA.

Price & Availability↑

CloneID	OAb21766
Clone ID Related Accession (Same CDS sequence)	NM_125208.3
Accession Version	NM_125208.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Transducin/WD40 repeat-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Apr 18, 2007 this sequence version replaced NM_125208.2.

ATGGGGAAAG ACGAAGAGGA AATGCGAGGT GAGATCGAGG AGCGACTAAT CAATGAAGAG 
TACAAGATCT GGAAGAAGAA CACTCCTTTC CTCTACGATC TCGTTATTAC TCACGCTCTC 
GAGTGGCCTT CACTTACTGT TGAATGGCTT CCTGACCGTG AAGAACCTTC CGGTAAAGAC 
TACTCCGTTC AGAAGATGAT TCTCGGTACC CATACCTCCG AGAGCGAGCC CAATTACTTG 
ATGCTAGCCC AAGTTCAGCT TCCTCTTGAC GATACCGAGA GTGAAGCTCG TCAGTACGAT 
GATGACCGCT CTGAATTTGG TGGCTTTGGC TGTGCAACTG GAAAGGTACA AATTATCCAG 
CAGATAAATC ATGATGGTGA GGTTAATCGA GCTCGCTATA TGCCTCAAAA CCCTTTCATA 
ATTGCTACCA AGACAGTTAA TGCGGAGGTC TATGTTTTCG ATTACAGTAA GCATCCATCT 
AAGCCTCCTC TTGATGGGGC TTGTAATCCT GATTTAAAGC TTAGAGGTCA CAGCTCTGAG 
GGATATGGTC TCTCTTGGAG TAAGTTCAAG CAGGGTCATT TGCTTAGTGG CTCTGATGAT 
GCTCAGATCT GCTTGTGGGA TATTAACGCT ACTCCTAAAA ACAAATCTCT GGACGCTCAG 
CAGATTTTTA AGGCTCATGA AGGGGTTGTG GAAGATGTTG CATGGCATCT GAGACATGAG 
TACCTGTTTG GATCAGTTGG AGATGACCAA TACCTTCTCA TATGGGATCT GCGTTCTCCA 
TCTGCCAGTA AACCAGTACA GTCTGTGGTT GCTCATTCGA TGGAGGTTAA CTGCTTAGCC 
TTCAATCCAT TTAATGAGTG GGTTGTGGCA ACAGGATCAA CTGACAAGAC TGTTAAATTA 
TTCGATCTAC GGAAGCTAAG CACCGCTCTT CACACATTTG ATAGCCACAA AGAGGAGGTT 
TTTCAAGTTG GTTGGAACCC AAAGAACGAG ACTATTTTAG CTTCATGTTG CCTTGGTAGA 
AGACTTATGG TTTGGGACCT TAGCAGGATC GATGAGGAAC AGACAGTGGA GGACGCAGAA 
GACGGCCCAC CTGAGCTGCT GTTTATTCAC GGTGGTCACA CTAGCAAAAT TTCAGATTTC 
TCGTGGAACC CTTGTGAAGA TTGGGTTATT TCCAGTGTTG CTGAGGACAA CATACTCCAA 
ATATGGCAAA TGGCTGAAAA CATCTATCAT GATGAGGATG ACGCTCCAGG GGAAGAACCA 
TCAAAAGCTT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200631.1
CDS	101..1375
Translation	MGKDEEEMRGEIEERLINEEYKIWKKNTPFLYDLVITHALEWPSLTVEWLPDREEPSGKDYSVQKMILGTHTSESEPNYLMLAQVQLPLDDTESEARQYDDDRSEFGGFGCATGKVQIIQQINHDGEVNRARYMPQNPFIIATKTVNAEVYVFDYSKHPSKPPLDGACNPDLKLRGHSSEGYGLSWSKFKQGHLLSGSDDAQICLWDINATPKNKSLDAQQIFKAHEGVVEDVAWHLRHEYLFGSVGDDQYLLIWDLRSPSASKPVQSVVAHSMEVNCLAFNPFNEWVVATGSTDKTVKLFDLRKLSTALHTFDSHKEEVFQVGWNPKNETILASCCLGRRLMVWDLSRIDEEQTVEDAEDGPPELLFIHGGHTSKISDFSWNPCEDWVISSVAEDNILQIWQMAENIYHDEDDAPGEEPSKAS

Target ORF information:

RefSeq Version	NM_125208.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Transducin/WD40 repeat-like superfamily protein (MSI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_125208.3

ORF Insert Sequence:

ATGGGGAAAG ACGAAGAGGA AATGCGAGGT GAGATCGAGG AGCGACTAAT CAATGAAGAG 
TACAAGATCT GGAAGAAGAA CACTCCTTTC CTCTACGATC TCGTTATTAC TCACGCTCTC 
GAGTGGCCTT CACTTACTGT TGAATGGCTT CCTGACCGTG AAGAACCTTC CGGTAAAGAC 
TACTCCGTTC AGAAGATGAT TCTCGGTACC CATACCTCCG AGAGCGAGCC CAATTACTTG 
ATGCTAGCCC AAGTTCAGCT TCCTCTTGAC GATACCGAGA GTGAAGCTCG TCAGTACGAT 
GATGACCGCT CTGAATTTGG TGGCTTTGGC TGTGCAACTG GAAAGGTACA AATTATCCAG 
CAGATAAATC ATGATGGTGA GGTTAATCGA GCTCGCTATA TGCCTCAAAA CCCTTTCATA 
ATTGCTACCA AGACAGTTAA TGCGGAGGTC TATGTTTTCG ATTACAGTAA GCATCCATCT 
AAGCCTCCTC TTGATGGGGC TTGTAATCCT GATTTAAAGC TTAGAGGTCA CAGCTCTGAG 
GGATATGGTC TCTCTTGGAG TAAGTTCAAG CAGGGTCATT TGCTTAGTGG CTCTGATGAT 
GCTCAGATCT GCTTGTGGGA TATTAACGCT ACTCCTAAAA ACAAATCTCT GGACGCTCAG 
CAGATTTTTA AGGCTCATGA AGGGGTTGTG GAAGATGTTG CATGGCATCT GAGACATGAG 
TACCTGTTTG GATCAGTTGG AGATGACCAA TACCTTCTCA TATGGGATCT GCGTTCTCCA 
TCTGCCAGTA AACCAGTACA GTCTGTGGTT GCTCATTCGA TGGAGGTTAA CTGCTTAGCC 
TTCAATCCAT TTAATGAGTG GGTTGTGGCA ACAGGATCAA CTGACAAGAC TGTTAAATTA 
TTCGATCTAC GGAAGCTAAG CACCGCTCTT CACACATTTG ATAGCCACAA AGAGGAGGTT 
TTTCAAGTTG GTTGGAACCC AAAGAACGAG ACTATTTTAG CTTCATGTTG CCTTGGTAGA 
AGACTTATGG TTTGGGACCT TAGCAGGATC GATGAGGAAC AGACAGTGGA GGACGCAGAA 
GACGGCCCAC CTGAGCTGCT GTTTATTCAC GGTGGTCACA CTAGCAAAAT TTCAGATTTC 
TCGTGGAACC CTTGTGAAGA TTGGGTTATT TCCAGTGTTG CTGAGGACAA CATACTCCAA 
ATATGGCAAA TGGCTGAAAA CATCTATCAT GATGAGGATG ACGCTCCAGG GGAAGAACCA 
TCAAAAGCTT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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MSI1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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