HSP81-2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSP81-2
Entrez Gene ID	835701
Full Name	heat shock protein 81-2
Synonyms	AtHsp90.2,EARLY-RESPONSIVE TO DEHYDRATION 8,ERD8,HEAT SHOCK PROTEIN 90.2,HEAT SHOCK PROTEIN 81-2,HEAT SHOCK PROTEIN 90.2,HSP81.2,HSP90.2,MDA7.7,MDA7_7,heat shock protein 81-2,heat shock protein 81.2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001203624.1	NP_001190553.1	heat shock protein 81-2
NM_124985.5	NP_200414.1	heat shock protein 81-2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HSP90AA1	XP_005623992.1
Arabidopsis thaliana (thale cress)	HSP81-3	NP_200412.1
Arabidopsis thaliana (thale cress)	HSP81-2	NP_200414.1
Oryza sativa (rice)	Os09g0482100	NP_001063500.1
	HSP90AA1	XP_002805263.1
Arabidopsis thaliana (thale cress)	Hsp81.4	NP_200411.1
Oryza sativa (rice)	Os09g0482400	NP_001063503.1
Pan troglodytes (chimpanzee)	HSP90AA1	NP_001092042.1
Mus musculus (house mouse)	Hsp90aa1	NP_034610.1
Rattus norvegicus (Norway rat)	Hsp90aa1	NP_786937.1
Bos taurus (cattle)	HSP90AA1	NP_001012688.1
Gallus gallus (chicken)	HSP90AA1	NP_001103255.1
Oryza sativa (rice)	Os09g0482610	NP_001175894.1
Homo sapiens (human)	HSP90AA1	NP_001017963.2
Danio rerio (zebrafish)	hsp90aa1.2	NP_001038538.1
Danio rerio (zebrafish)	hsp90aa1.1	NP_571403.1
Xenopus tropicalis (tropical clawed frog)	hsp90aa1.1	NP_001016282.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that the induction expression of ABA-responsive genes under salt and drought stress was delayed by constitutive overexpression of cytosolic AtHsp90.2, but was hardly affected by that of organellar AtHsp90.5 and AtHsp90.7.
Title: Overexpression of AtHsp90.2, AtHsp90.5 and AtHsp90.7 in Arabidopsis thaliana enhances plant sensitivity to salt and drought stresses.

Nuclear magnetic resonance spectroscopy and mutational analysis of HSP90 revealed the nature of its binding with the CS domain of SGT1 which is required for innate immunity in plants and animals. [HSP90]
Title: Structural and functional analysis of SGT1-HSP90 core complex required for innate immunity in plants.

HSP90.2 is involved in the heat shock response in A. thaliana. It interacts with AtHsfA7a and AtHsfB1 but only weakly interacts or does not interact with AtHsfA4c.
Title: Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana.

The regulation of AtHSP90-1 and AtHSP90-3 proteins during seed development in A. thaliana. [HSP90-3]
Title: Tight regulation of expression of two Arabidopsis cytosolic Hsp90 genes during embryo development.

The following HSP81-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSP81-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb21548	NM_124985.5 Latest version!	Arabidopsis thaliana heat shock protein 81-2 (HSP81-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb30974	NM_001203624.1 Latest version!	Arabidopsis thaliana heat shock protein 81-2 (HSP81-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSP81-2 ( NM_124985.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200414.1 Arabidopsis thaliana heat shock protein 81-2 (HSP81-2), mRNA.

Price & Availability↑

CloneID	OAb21548
Clone ID Related Accession (Same CDS sequence)	NM_124985.5
Accession Version	NM_124985.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heat shock protein 81-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_124985.4.

ATGGCGGACG CTGAAACCTT TGCTTTCCAA GCTGAGATCA ACCAGTTGCT TTCCCTCATC 
ATCAATACCT TTTACTCTAA CAAGGAGATC TTCCTCCGTG AACTCATCAG TAACTCTTCT 
GATGCTTTGG ACAAGATTAG ATTCGAGTCC TTGACGGACA AGAGCAAGCT CGATGGTCAG 
CCTGAGCTCT TCATTCACAT CATTCCCGAC AAGACTAACA ACACCTTGAC CATTATTGAT 
AGTGGTATTG GCATGACCAA GGCTGATTTG GTCAACAACC TTGGAACAAT TGCAAGATCT 
GGAACCAAGG AATTCATGGA AGCACTGGCT GCTGGAGCTG ATGTTAGCAT GATTGGTCAG 
TTTGGTGTTG GTTTCTACTC TGCTTACTTG GTTGCTGACA AGGTTGTTGT TACTACCAAG 
CACAATGACG ATGAGCAGTA CGTGTGGGAG TCTCAGGCCG GGGGATCTTT CACTGTGACC 
AGAGACACCT CTGGGGAGAC TCTTGGTAGA GGAACAAAGA TGGTTCTTTA CCTCAAGGAA 
GACCAGTTGG AGTACCTTGA GGAGCGAAGG CTTAAGGATT TGGTGAAGAA GCACTCTGAG 
TTCATCAGCT ACCCAATCTC TCTCTGGATT GAGAAGACCA TTGAGAAGGA GATCTCTGAT 
GATGAAGAGG AGGAAGAGAA GAAGGATGAG GAAGGCAAGG TTGAGGAAGT TGATGAGGAG 
AAAGAAAAGG AGGAGAAGAA AAAGAAGAAG ATTAAGGAGG TTTCTCATGA GTGGGATTTG 
GTGAACAAGC AGAAGCCTAT CTGGATGAGG AAGCCAGAGG AGATCAACAA GGAGGAGTAC 
GCTGCTTTCT ACAAGAGTCT CAGCAATGAT TGGGAGGAGC ATTTGGCTGT GAAGCATTTC 
TCTGTTGAAG GACAGCTTGA GTTCAAAGCT ATCCTCTTTG TTCCCAAGAG AGCTCCTTTT 
GATCTCTTTG ACACTAAGAA GAAGCCCAAC AACATCAAGC TCTATGTCCG TCGTGTCTTC 
ATCATGGACA ACTGTGAAGA CATCATCCCT GAGTACCTCG GATTTGTGAA AGGTATTGTC 
GACTCTGAAG ATCTTCCTCT CAACATCTCG AGAGAGACAT TGCAGCAGAA CAAGATCCTC 
AAGGTCATCC GCAAGAACCT TGTGAAGAAG TGCCTTGAGC TCTTCTTTGA GATTGCTGAG 
AACAAGGAAG ACTACAACAA GTTCTACGAG GCTTTCTCTA AGAACCTGAA GCTCGGTATC 
CATGAGGACT CCCAAAACAG AACCAAGATT GCTGAGTTGC TCCGTTACCA CTCAACCAAG 
AGCGGTGATG AATTGACTAG TCTCAAGGAC TACGTGACAA GGATGAAGGA AGGTCAGAAC 
GATATCTTCT ACATCACTGG TGAGAGCAAG AAGGCTGTTG AGAACTCTCC ATTCCTTGAG 
AAGCTCAAGA AGAAAGGTAT TGAAGTTCTC TACATGGTTG ATGCGATTGA TGAGTACGCT 
ATTGGTCAGC TTAAGGAATT CGAGGGAAAG AAACTTGTTT CTGCAACCAA GGAAGGTCTG 
AAACTGGATG AGACCGAAGA TGAAAAGAAG AAGAAAGAAG AGCTCAAGGA GAAGTTTGAA 
GGACTCTGCA AAGTGATCAA AGACGTTTTG GGTGACAAGG TCGAGAAGGT TATTGTTTCT 
GACCGTGTTG TGGACTCACC GTGCTGTCTT GTAACCGGCG AATACGGCTG GACTGCAAAC 
ATGGAGAGGA TCATGAAAGC TCAAGCCTTG AGAGACAGCA GCATGGCTGG TTACATGTCG 
AGTAAGAAGA CAATGGAGAT TAATCCAGAG AACTCCATCA TGGATGAGTT GAGAAAGCGA 
GCTGACGCAG ACAAGAACGA CAAGTCTGTG AAGGACCTTG TGCTTCTCCT CTTTGAGACC 
GCTCTTCTCA CTTCTGGTTT CAGCCTTGAT GAGCCAAACA CTTTCGGAAG CAGGATCCAC 
AGGATGCTGA AGCTTGGACT GAGCATTGAT GACGATGATG CTGTTGAAGC CGATGCTGAG 
ATGCCTCCCC TTGAAGACGA TGCTGATGCC GAAGGTAGCA AGATGGAGGA AGTCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200414.1
CDS	451..2550
Translation	MADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQPELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEALAAGADVSMIGQFGVGFYSAYLVADKVVVTTKHNDDEQYVWESQAGGSFTVTRDTSGETLGRGTKMVLYLKEDQLEYLEERRLKDLVKKHSEFISYPISLWIEKTIEKEISDDEEEEEKKDEEGKVEEVDEEKEKEEKKKKKIKEVSHEWDLVNKQKPIWMRKPEEINKEEYAAFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTKKKPNNIKLYVRRVFIMDNCEDIIPEYLGFVKGIVDSEDLPLNISRETLQQNKILKVIRKNLVKKCLELFFEIAENKEDYNKFYEAFSKNLKLGIHEDSQNRTKIAELLRYHSTKSGDELTSLKDYVTRMKEGQNDIFYITGESKKAVENSPFLEKLKKKGIEVLYMVDAIDEYAIGQLKEFEGKKLVSATKEGLKLDETEDEKKKKEELKEKFEGLCKVIKDVLGDKVEKVIVSDRVVDSPCCLVTGEYGWTANMERIMKAQALRDSSMAGYMSSKKTMEINPENSIMDELRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIDDDDAVEADAEMPPLEDDADAEGSKMEEVD

Target ORF information:

RefSeq Version	NM_124985.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heat shock protein 81-2 (HSP81-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124985.5

ORF Insert Sequence:

ATGGCGGACG CTGAAACCTT TGCTTTCCAA GCTGAGATCA ACCAGTTGCT TTCCCTCATC 
ATCAATACCT TTTACTCTAA CAAGGAGATC TTCCTCCGTG AACTCATCAG TAACTCTTCT 
GATGCTTTGG ACAAGATTAG ATTCGAGTCC TTGACGGACA AGAGCAAGCT CGATGGTCAG 
CCTGAGCTCT TCATTCACAT CATTCCCGAC AAGACTAACA ACACCTTGAC CATTATTGAT 
AGTGGTATTG GCATGACCAA GGCTGATTTG GTCAACAACC TTGGAACAAT TGCAAGATCT 
GGAACCAAGG AATTCATGGA AGCACTGGCT GCTGGAGCTG ATGTTAGCAT GATTGGTCAG 
TTTGGTGTTG GTTTCTACTC TGCTTACTTG GTTGCTGACA AGGTTGTTGT TACTACCAAG 
CACAATGACG ATGAGCAGTA CGTGTGGGAG TCTCAGGCCG GGGGATCTTT CACTGTGACC 
AGAGACACCT CTGGGGAGAC TCTTGGTAGA GGAACAAAGA TGGTTCTTTA CCTCAAGGAA 
GACCAGTTGG AGTACCTTGA GGAGCGAAGG CTTAAGGATT TGGTGAAGAA GCACTCTGAG 
TTCATCAGCT ACCCAATCTC TCTCTGGATT GAGAAGACCA TTGAGAAGGA GATCTCTGAT 
GATGAAGAGG AGGAAGAGAA GAAGGATGAG GAAGGCAAGG TTGAGGAAGT TGATGAGGAG 
AAAGAAAAGG AGGAGAAGAA AAAGAAGAAG ATTAAGGAGG TTTCTCATGA GTGGGATTTG 
GTGAACAAGC AGAAGCCTAT CTGGATGAGG AAGCCAGAGG AGATCAACAA GGAGGAGTAC 
GCTGCTTTCT ACAAGAGTCT CAGCAATGAT TGGGAGGAGC ATTTGGCTGT GAAGCATTTC 
TCTGTTGAAG GACAGCTTGA GTTCAAAGCT ATCCTCTTTG TTCCCAAGAG AGCTCCTTTT 
GATCTCTTTG ACACTAAGAA GAAGCCCAAC AACATCAAGC TCTATGTCCG TCGTGTCTTC 
ATCATGGACA ACTGTGAAGA CATCATCCCT GAGTACCTCG GATTTGTGAA AGGTATTGTC 
GACTCTGAAG ATCTTCCTCT CAACATCTCG AGAGAGACAT TGCAGCAGAA CAAGATCCTC 
AAGGTCATCC GCAAGAACCT TGTGAAGAAG TGCCTTGAGC TCTTCTTTGA GATTGCTGAG 
AACAAGGAAG ACTACAACAA GTTCTACGAG GCTTTCTCTA AGAACCTGAA GCTCGGTATC 
CATGAGGACT CCCAAAACAG AACCAAGATT GCTGAGTTGC TCCGTTACCA CTCAACCAAG 
AGCGGTGATG AATTGACTAG TCTCAAGGAC TACGTGACAA GGATGAAGGA AGGTCAGAAC 
GATATCTTCT ACATCACTGG TGAGAGCAAG AAGGCTGTTG AGAACTCTCC ATTCCTTGAG 
AAGCTCAAGA AGAAAGGTAT TGAAGTTCTC TACATGGTTG ATGCGATTGA TGAGTACGCT 
ATTGGTCAGC TTAAGGAATT CGAGGGAAAG AAACTTGTTT CTGCAACCAA GGAAGGTCTG 
AAACTGGATG AGACCGAAGA TGAAAAGAAG AAGAAAGAAG AGCTCAAGGA GAAGTTTGAA 
GGACTCTGCA AAGTGATCAA AGACGTTTTG GGTGACAAGG TCGAGAAGGT TATTGTTTCT 
GACCGTGTTG TGGACTCACC GTGCTGTCTT GTAACCGGCG AATACGGCTG GACTGCAAAC 
ATGGAGAGGA TCATGAAAGC TCAAGCCTTG AGAGACAGCA GCATGGCTGG TTACATGTCG 
AGTAAGAAGA CAATGGAGAT TAATCCAGAG AACTCCATCA TGGATGAGTT GAGAAAGCGA 
GCTGACGCAG ACAAGAACGA CAAGTCTGTG AAGGACCTTG TGCTTCTCCT CTTTGAGACC 
GCTCTTCTCA CTTCTGGTTT CAGCCTTGAT GAGCCAAACA CTTTCGGAAG CAGGATCCAC 
AGGATGCTGA AGCTTGGACT GAGCATTGAT GACGATGATG CTGTTGAAGC CGATGCTGAG 
ATGCCTCCCC TTGAAGACGA TGCTGATGCC GAAGGTAGCA AGATGGAGGA AGTCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSP81-2 ( NM_001203624.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190553.1 Arabidopsis thaliana heat shock protein 81-2 (HSP81-2), mRNA.

Price & Availability↑

CloneID	OAb30974
Clone ID Related Accession (Same CDS sequence)	NM_001203624.1
Accession Version	NM_001203624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heat shock protein 81-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGCGGACG CTGAAACCTT TGCTTTCCAA GCTGAGATCA ACCAGTTGCT TTCCCTCATC 
ATCAATACCT TTTACTCTAA CAAGGAGATC TTCCTCCGTG AACTCATCAG TAACTCTTCT 
GATGTACGAT CTCTATCTCT CTCTACCTTA TTGTATACTT GTGTATCTCG TTTGATTGAT 
TTGGCGGATG GATCTGTAGA TTTAGGTTTG GCTTTGGACA AGATTAGATT CGAGTCCTTG 
ACGGACAAGA GCAAGCTCGA TGGTCAGCCT GAGCTCTTCA TTCACATCAT TCCCGACAAG 
ACTAACAACA CCTTGACCAT TATTGATAGT GGTATTGGCA TGACCAAGGC TGATTTGGTC 
AACAACCTTG GAACAATTGC AAGATCTGGA ACCAAGGAAT TCATGGAAGC ACTGGCTGCT 
GGAGCTGATG TTAGCATGAT TGGTCAGTTT GGTGTTGGTT TCTACTCTGC TTACTTGGTT 
GCTGACAAGG TTGTTGTTAC TACCAAGCAC AATGACGATG AGCAGTACGT GTGGGAGTCT 
CAGGCCGGGG GATCTTTCAC TGTGACCAGA GACACCTCTG GGGAGACTCT TGGTAGAGGA 
ACAAAGATGG TTCTTTACCT CAAGGAAGAC CAGTTGGAGT ACCTTGAGGA GCGAAGGCTT 
AAGGATTTGG TGAAGAAGCA CTCTGAGTTC ATCAGCTACC CAATCTCTCT CTGGATTGAG 
AAGACCATTG AGAAGGAGAT CTCTGATGAT GAAGAGGAGG AAGAGAAGAA GGATGAGGAA 
GGCAAGGTTG AGGAAGTTGA TGAGGAGAAA GAAAAGGAGG AGAAGAAAAA GAAGAAGATT 
AAGGAGGTTT CTCATGAGTG GGATTTGGTG AACAAGCAGA AGCCTATCTG GATGAGGAAG 
CCAGAGGAGA TCAACAAGGA GGAGTACGCT GCTTTCTACA AGAGTCTCAG CAATGATTGG 
GAGGAGCATT TGGCTGTGAA GCATTTCTCT GTTGAAGGAC AGCTTGAGTT CAAAGCTATC 
CTCTTTGTTC CCAAGAGAGC TCCTTTTGAT CTCTTTGACA CTAAGAAGAA GCCCAACAAC 
ATCAAGCTCT ATGTCCGTCG TGTCTTCATC ATGGACAACT GTGAAGACAT CATCCCTGAG 
TACCTCGGAT TTGTGAAAGG TATTGTCGAC TCTGAAGATC TTCCTCTCAA CATCTCGAGA 
GAGACATTGC AGCAGAACAA GATCCTCAAG GTCATCCGCA AGAACCTTGT GAAGAAGTGC 
CTTGAGCTCT TCTTTGAGAT TGCTGAGAAC AAGGAAGACT ACAACAAGTT CTACGAGGCT 
TTCTCTAAGA ACCTGAAGCT CGGTATCCAT GAGGACTCCC AAAACAGAAC CAAGATTGCT 
GAGTTGCTCC GTTACCACTC AACCAAGAGC GGTGATGAAT TGACTAGTCT CAAGGACTAC 
GTGACAAGGA TGAAGGAAGG TCAGAACGAT ATCTTCTACA TCACTGGTGA GAGCAAGAAG 
GCTGTTGAGA ACTCTCCATT CCTTGAGAAG CTCAAGAAGA AAGGTATTGA AGTTCTCTAC 
ATGGTTGATG CGATTGATGA GTACGCTATT GGTCAGCTTA AGGAATTCGA GGGAAAGAAA 
CTTGTTTCTG CAACCAAGGA AGGTCTGAAA CTGGATGAGA CCGAAGATGA AAAGAAGAAG 
AAAGAAGAGC TCAAGGAGAA GTTTGAAGGA CTCTGCAAAG TGATCAAAGA CGTTTTGGGT 
GACAAGGTCG AGAAGGTTAT TGTTTCTGAC CGTGTTGTGG ACTCACCGTG CTGTCTTGTA 
ACCGGCGAAT ACGGCTGGAC TGCAAACATG GAGAGGATCA TGAAAGCTCA AGCCTTGAGA 
GACAGCAGCA TGGCTGGTTA CATGTCGAGT AAGAAGACAA TGGAGATTAA TCCAGAGAAC 
TCCATCATGG ATGAGTTGAG AAAGCGAGCT GACGCAGACA AGAACGACAA GTCTGTGAAG 
GACCTTGTGC TTCTCCTCTT TGAGACCGCT CTTCTCACTT CTGGTTTCAG CCTTGATGAG 
CCAAACACTT TCGGAAGCAG GATCCACAGG ATGCTGAAGC TTGGACTGAG CATTGATGAC 
GATGATGCTG TTGAAGCCGA TGCTGAGATG CCTCCCCTTG AAGACGATGC TGATGCCGAA 
GGTAGCAAGA TGGAGGAAGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190553.1
CDS	92..2278
Translation	MADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDVRSLSLSTLLYTCVSRLIDLADGSVDLGLALDKIRFESLTDKSKLDGQPELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEALAAGADVSMIGQFGVGFYSAYLVADKVVVTTKHNDDEQYVWESQAGGSFTVTRDTSGETLGRGTKMVLYLKEDQLEYLEERRLKDLVKKHSEFISYPISLWIEKTIEKEISDDEEEEEKKDEEGKVEEVDEEKEKEEKKKKKIKEVSHEWDLVNKQKPIWMRKPEEINKEEYAAFYKSLSNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTKKKPNNIKLYVRRVFIMDNCEDIIPEYLGFVKGIVDSEDLPLNISRETLQQNKILKVIRKNLVKKCLELFFEIAENKEDYNKFYEAFSKNLKLGIHEDSQNRTKIAELLRYHSTKSGDELTSLKDYVTRMKEGQNDIFYITGESKKAVENSPFLEKLKKKGIEVLYMVDAIDEYAIGQLKEFEGKKLVSATKEGLKLDETEDEKKKKEELKEKFEGLCKVIKDVLGDKVEKVIVSDRVVDSPCCLVTGEYGWTANMERIMKAQALRDSSMAGYMSSKKTMEINPENSIMDELRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIDDDDAVEADAEMPPLEDDADAEGSKMEEVD

Target ORF information:

RefSeq Version	NM_001203624.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heat shock protein 81-2 (HSP81-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203624.1

ORF Insert Sequence:

ATGGCGGACG CTGAAACCTT TGCTTTCCAA GCTGAGATCA ACCAGTTGCT TTCCCTCATC 
ATCAATACCT TTTACTCTAA CAAGGAGATC TTCCTCCGTG AACTCATCAG TAACTCTTCT 
GATGTACGAT CTCTATCTCT CTCTACCTTA TTGTATACTT GTGTATCTCG TTTGATTGAT 
TTGGCGGATG GATCTGTAGA TTTAGGTTTG GCTTTGGACA AGATTAGATT CGAGTCCTTG 
ACGGACAAGA GCAAGCTCGA TGGTCAGCCT GAGCTCTTCA TTCACATCAT TCCCGACAAG 
ACTAACAACA CCTTGACCAT TATTGATAGT GGTATTGGCA TGACCAAGGC TGATTTGGTC 
AACAACCTTG GAACAATTGC AAGATCTGGA ACCAAGGAAT TCATGGAAGC ACTGGCTGCT 
GGAGCTGATG TTAGCATGAT TGGTCAGTTT GGTGTTGGTT TCTACTCTGC TTACTTGGTT 
GCTGACAAGG TTGTTGTTAC TACCAAGCAC AATGACGATG AGCAGTACGT GTGGGAGTCT 
CAGGCCGGGG GATCTTTCAC TGTGACCAGA GACACCTCTG GGGAGACTCT TGGTAGAGGA 
ACAAAGATGG TTCTTTACCT CAAGGAAGAC CAGTTGGAGT ACCTTGAGGA GCGAAGGCTT 
AAGGATTTGG TGAAGAAGCA CTCTGAGTTC ATCAGCTACC CAATCTCTCT CTGGATTGAG 
AAGACCATTG AGAAGGAGAT CTCTGATGAT GAAGAGGAGG AAGAGAAGAA GGATGAGGAA 
GGCAAGGTTG AGGAAGTTGA TGAGGAGAAA GAAAAGGAGG AGAAGAAAAA GAAGAAGATT 
AAGGAGGTTT CTCATGAGTG GGATTTGGTG AACAAGCAGA AGCCTATCTG GATGAGGAAG 
CCAGAGGAGA TCAACAAGGA GGAGTACGCT GCTTTCTACA AGAGTCTCAG CAATGATTGG 
GAGGAGCATT TGGCTGTGAA GCATTTCTCT GTTGAAGGAC AGCTTGAGTT CAAAGCTATC 
CTCTTTGTTC CCAAGAGAGC TCCTTTTGAT CTCTTTGACA CTAAGAAGAA GCCCAACAAC 
ATCAAGCTCT ATGTCCGTCG TGTCTTCATC ATGGACAACT GTGAAGACAT CATCCCTGAG 
TACCTCGGAT TTGTGAAAGG TATTGTCGAC TCTGAAGATC TTCCTCTCAA CATCTCGAGA 
GAGACATTGC AGCAGAACAA GATCCTCAAG GTCATCCGCA AGAACCTTGT GAAGAAGTGC 
CTTGAGCTCT TCTTTGAGAT TGCTGAGAAC AAGGAAGACT ACAACAAGTT CTACGAGGCT 
TTCTCTAAGA ACCTGAAGCT CGGTATCCAT GAGGACTCCC AAAACAGAAC CAAGATTGCT 
GAGTTGCTCC GTTACCACTC AACCAAGAGC GGTGATGAAT TGACTAGTCT CAAGGACTAC 
GTGACAAGGA TGAAGGAAGG TCAGAACGAT ATCTTCTACA TCACTGGTGA GAGCAAGAAG 
GCTGTTGAGA ACTCTCCATT CCTTGAGAAG CTCAAGAAGA AAGGTATTGA AGTTCTCTAC 
ATGGTTGATG CGATTGATGA GTACGCTATT GGTCAGCTTA AGGAATTCGA GGGAAAGAAA 
CTTGTTTCTG CAACCAAGGA AGGTCTGAAA CTGGATGAGA CCGAAGATGA AAAGAAGAAG 
AAAGAAGAGC TCAAGGAGAA GTTTGAAGGA CTCTGCAAAG TGATCAAAGA CGTTTTGGGT 
GACAAGGTCG AGAAGGTTAT TGTTTCTGAC CGTGTTGTGG ACTCACCGTG CTGTCTTGTA 
ACCGGCGAAT ACGGCTGGAC TGCAAACATG GAGAGGATCA TGAAAGCTCA AGCCTTGAGA 
GACAGCAGCA TGGCTGGTTA CATGTCGAGT AAGAAGACAA TGGAGATTAA TCCAGAGAAC 
TCCATCATGG ATGAGTTGAG AAAGCGAGCT GACGCAGACA AGAACGACAA GTCTGTGAAG 
GACCTTGTGC TTCTCCTCTT TGAGACCGCT CTTCTCACTT CTGGTTTCAG CCTTGATGAG 
CCAAACACTT TCGGAAGCAG GATCCACAGG ATGCTGAAGC TTGGACTGAG CATTGATGAC 
GATGATGCTG TTGAAGCCGA TGCTGAGATG CCTCCCCTTG AAGACGATGC TGATGCCGAA 
GGTAGCAAGA TGGAGGAAGT CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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HSP81-2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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