TOP1ALPHA cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP1ALPHA
Entrez Gene ID	835623
Full Name	DNA topoisomerase I alpha
Synonyms	DNA topoisomerase 1 beta,DNA topoisomerase I alpha,MGO1,MGOUN 1,MTE17.1,MTE17_1,TOP1,TOP1BETA,TOPOISOMERASE 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001203615.1	NP_001190544.1	DNA topoisomerase I alpha
NM_001203614.1	NP_001190543.1	DNA topoisomerase I alpha
NM_124912.5	NP_200341.1	DNA topoisomerase I alpha

Pathways from BioSystems

Homologies

	TOP1	XP_001088021.1
Bos taurus (cattle)	TOP1	NP_001193416.1
Gallus gallus (chicken)	TOP1	NP_990441.1
Neurospora crassa	NCU09118	XP_958033.2
Arabidopsis thaliana (thale cress)	TOP1ALPHA	NP_200341.1
Canis lupus familiaris (dog)	TOP1	XP_534420.3
Caenorhabditis elegans (roundworm)	top-1	NP_001021578.1
Saccharomyces cerevisiae (baker's yeast)	TOP1	NP_014637.1
Magnaporthe oryzae (rice blast fungus)	MGG_09917	XP_003710015.1
Arabidopsis thaliana (thale cress)	TOP1BETA	NP_200342.1
Kluyveromyces lactis	KLLA0C06116g	XP_452470.1
Eremothecium gossypii	AGOS_ACL008C	NP_983396.2
Xenopus tropicalis (tropical clawed frog)	LOC100496521	XP_002932918.2
Mus musculus (house mouse)	Top1	NP_033434.2
Danio rerio (zebrafish)	top1l	NP_001037789.1
Oryza sativa (rice)	Os08g0154600	NP_001061015.1
Homo sapiens (human)	TOP1	NP_003277.1
Pan troglodytes (chimpanzee)	TOP1	XP_514649.2
Rattus norvegicus (Norway rat)	Top1	NP_072137.1
Drosophila melanogaster (fruit fly)	Top1	NP_001014742.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004078	XP_003436707.1
Schizosaccharomyces pombe (fission yeast)	top1	NP_596209.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TOP1ALPHA role in flowering
Title: DNA Topoisomerase I? Affects the Floral Transition.

Pleiotropic effect of the fas5 mutation on different stages of ontogeny and different organs suggests that the FAS5 gene plays a complex regulatory role at all stages of the A. thaliana shoot development. [Fas5]
Title: [Pleiotropic effect of the fas5 mutation on the shoot development of Arabidopsis thaliana].

Further studies with Arabidopsis top1alpha mutants showed that TOP1alpha silences endogenous RdDM loci by facilitating the production of Pol V-dependent long non-coding RNAs, AGONAUTE4 recruitment and H3K9me2 deposition at TEs and repeats
Title: DNA topoisomerase 1? promotes transcriptional silencing of transposable elements through DNA methylation and histone lysine 9 dimethylation in Arabidopsis.

In the floral meristem, the binding of AG to WUS is reduced in top1a-2, which results in reduced H3K27me3 levels at WUS and prolonged WUS expression, and consequently loss of floral determinacy.
Title: DNA topoisomerase I affects polycomb group protein-mediated epigenetic regulation and plant development by altering nucleosome distribution in Arabidopsis.

MGO1 encodes a putative Arabidopsis type IB topoisomerase that functions together with WUS in stem cell regulation. [MGO1]
Title: MGOUN1 encodes an Arabidopsis type IB DNA topoisomerase required in stem cell regulation and to maintain developmentally regulated gene silencing.

The following TOP1ALPHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1ALPHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb30964	NM_001203615.1 Latest version!	Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$342.30 $489.00
OAb21488	NM_124912.5 Latest version!	Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OAb30963	NM_001203614.1 Latest version!	Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP1ALPHA ( NM_001203615.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190544.1 Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.

Price & Availability↑

CloneID	OAb30964
Clone ID Related Accession (Same CDS sequence)	NM_001203615.1
Accession Version	NM_001203615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA topoisomerase I alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGGCACTG AAACAGTTTC AAAACCTGTG ATGGATAATG GGTCTGGAGA CAGTGATGAT 
GACAAGCCTT TAGCGTTCAA GAGGAATAAT ACAGTGGCTT CTAATTCGAA TCAATCTAAA 
TCCAATTCCC AGAGAAGCAA GGCAGTTCCT ACCACCAAGG TATCACCTAT GAGATCACCT 
GTGACTAGCC CAAATGGAAC CACTCCTTCG AATAAAACTT CTATAGTGAA ATCCTCTATG 
CCATCATCTT CTTCTAAGGC TTCACCAGCA AAGTCACCAT TGCGGAATGA TATGCCCTCT 
ACTGTTAAGG ATAGGAGCCA GTTACAGAAA GATCAGTCTG AATGTAAAAT TGAGCATGAG 
GATTCTGAGG ATGATAGACC TTTAAGTTCC ATACTATCTG GAAATAAAGG GCCAACCTCT 
TCGCGGCAGG TTTCTTCACC GCAGCCAGAG AAAAAGAATA ATGGTGATCG ACCTCTTGAT 
AGAGCAAGCA GAATTATAAA AGACGAGTCA GATGATGAAA CTCCAATCTC GTCAATGTTT 
CGGAAGAAGA TTGATAGTGG GATGTCAGGG GGCAACCAGC TCTCCAATGA TGAAAAGAAA 
CCTTTGGTTC AAAAGTTGCA TCAGAATGGT TCCACAGTTA AGAATGAAGT GCCGAATGGT 
AAGGTATTAG GTAAGAGACC TCTGGAGAAG AATAGTTCTG CAGATCAATC TTCCTTGAAG 
AAGGCCAAAA TATCAGCTTC ACCTACTTCA GTGAAAATGA AACAAGATTC TGTCAAGAAA 
GAGATAGATG ATAAGGGAAG GGTTTTGGTT TCACCAAAAA TGAAAGCTAA ACAGTTATCT 
ACCAGAGAAG ATGGAACTGA TGATGATGAT GATGATGATG ATGATGATGA GGCCGACACT 
GTTGGTTGTT GTACTTTAAA AGTTGGAAAT GTGGAGTGTA TTCCTCCAAA TAAAATAAAG 
TTTGATTTTC TTGGTAAGGA TTCAATCCAG TATGTGAACA CAGTCGAGGT TGAGCCTCTT 
GTTTACAAGG CCATTGGACA GTTCCAGGCG GGAAAATCCA AAACAGATGA TCTTTTTGAT 
GAGTTAGACA CAAGTAAACT GAATGCTCAT TTGAAGGAAC TTGTGCCTGG TCTCACAGCT 
AAAGTATTCC GTACATATAA TGCTTCCATC ACTTTGGATG AAATGCTGAG TCAAGAAACC 
AAAGATGGCG ATGTTACTCA AAAAATAGTA GTTTATCAGA AAGCAAATAA GGAGGTTGCC 
ATAATCTGCA ATCATCAGCG TACTGTATCA AAGACACATG GTGCGCAAAT TGAAAAATTG 
ACTGCGAGAA TAGAAGAACT CAAGGAGGTT CTTAAAGAGC TCAAAACTAA TCTTGACCGG 
GCTAAAAAGG GAAAACCACC ATTGGAAGGT TCTGATGGAA AGAAGATTAG AAGCTTAGAA 
CCAAACGCGT GGGAGAAGAA GATTGCTCAA CAGAGTGCTA AGATCGAGAA AATGGAACGA 
GACATGCATA CAAAAGAGGA CTTGAAAACA GTGGCACTGG GCACGTCTAA GATCAACTAC 
CTGGATCCTA GAATCACAGT TGCATGGTGC AAACGTCATG AAGTACCAAT CGAAAAGATA 
TTCACCAAGT CTCTTTTGGA GAAGTTTGCG TGGGCAATGG ATGTAGAACC CGAGTACAGA 
TTCTCGCGCC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190544.1
CDS	145..1839
Translation	MGTETVSKPVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMRSPVTSPNGTTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQLQKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNEVPNGKVLGKRPLEKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMKAKQLSTREDGTDDDDDDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFTKSLLEKFAWAMDVEPEYRFSRR

Target ORF information:

RefSeq Version	NM_001203615.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203615.1

ORF Insert Sequence:

ATGGGCACTG AAACAGTTTC AAAACCTGTG ATGGATAATG GGTCTGGAGA CAGTGATGAT 
GACAAGCCTT TAGCGTTCAA GAGGAATAAT ACAGTGGCTT CTAATTCGAA TCAATCTAAA 
TCCAATTCCC AGAGAAGCAA GGCAGTTCCT ACCACCAAGG TATCACCTAT GAGATCACCT 
GTGACTAGCC CAAATGGAAC CACTCCTTCG AATAAAACTT CTATAGTGAA ATCCTCTATG 
CCATCATCTT CTTCTAAGGC TTCACCAGCA AAGTCACCAT TGCGGAATGA TATGCCCTCT 
ACTGTTAAGG ATAGGAGCCA GTTACAGAAA GATCAGTCTG AATGTAAAAT TGAGCATGAG 
GATTCTGAGG ATGATAGACC TTTAAGTTCC ATACTATCTG GAAATAAAGG GCCAACCTCT 
TCGCGGCAGG TTTCTTCACC GCAGCCAGAG AAAAAGAATA ATGGTGATCG ACCTCTTGAT 
AGAGCAAGCA GAATTATAAA AGACGAGTCA GATGATGAAA CTCCAATCTC GTCAATGTTT 
CGGAAGAAGA TTGATAGTGG GATGTCAGGG GGCAACCAGC TCTCCAATGA TGAAAAGAAA 
CCTTTGGTTC AAAAGTTGCA TCAGAATGGT TCCACAGTTA AGAATGAAGT GCCGAATGGT 
AAGGTATTAG GTAAGAGACC TCTGGAGAAG AATAGTTCTG CAGATCAATC TTCCTTGAAG 
AAGGCCAAAA TATCAGCTTC ACCTACTTCA GTGAAAATGA AACAAGATTC TGTCAAGAAA 
GAGATAGATG ATAAGGGAAG GGTTTTGGTT TCACCAAAAA TGAAAGCTAA ACAGTTATCT 
ACCAGAGAAG ATGGAACTGA TGATGATGAT GATGATGATG ATGATGATGA GGCCGACACT 
GTTGGTTGTT GTACTTTAAA AGTTGGAAAT GTGGAGTGTA TTCCTCCAAA TAAAATAAAG 
TTTGATTTTC TTGGTAAGGA TTCAATCCAG TATGTGAACA CAGTCGAGGT TGAGCCTCTT 
GTTTACAAGG CCATTGGACA GTTCCAGGCG GGAAAATCCA AAACAGATGA TCTTTTTGAT 
GAGTTAGACA CAAGTAAACT GAATGCTCAT TTGAAGGAAC TTGTGCCTGG TCTCACAGCT 
AAAGTATTCC GTACATATAA TGCTTCCATC ACTTTGGATG AAATGCTGAG TCAAGAAACC 
AAAGATGGCG ATGTTACTCA AAAAATAGTA GTTTATCAGA AAGCAAATAA GGAGGTTGCC 
ATAATCTGCA ATCATCAGCG TACTGTATCA AAGACACATG GTGCGCAAAT TGAAAAATTG 
ACTGCGAGAA TAGAAGAACT CAAGGAGGTT CTTAAAGAGC TCAAAACTAA TCTTGACCGG 
GCTAAAAAGG GAAAACCACC ATTGGAAGGT TCTGATGGAA AGAAGATTAG AAGCTTAGAA 
CCAAACGCGT GGGAGAAGAA GATTGCTCAA CAGAGTGCTA AGATCGAGAA AATGGAACGA 
GACATGCATA CAAAAGAGGA CTTGAAAACA GTGGCACTGG GCACGTCTAA GATCAACTAC 
CTGGATCCTA GAATCACAGT TGCATGGTGC AAACGTCATG AAGTACCAAT CGAAAAGATA 
TTCACCAAGT CTCTTTTGGA GAAGTTTGCG TGGGCAATGG ATGTAGAACC CGAGTACAGA 
TTCTCGCGCC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP1ALPHA ( NM_124912.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200341.1 Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.

Price & Availability↑

CloneID	OAb21488
Clone ID Related Accession (Same CDS sequence)	NM_124912.5
Accession Version	NM_124912.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA topoisomerase I alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_124912.4.

ATGGGCACTG AAACAGTTTC AAAACCTGTG ATGGATAATG GGTCTGGAGA CAGTGATGAT 
GACAAGCCTT TAGCGTTCAA GAGGAATAAT ACAGTGGCTT CTAATTCGAA TCAATCTAAA 
TCCAATTCCC AGAGAAGCAA GGCAGTTCCT ACCACCAAGG TATCACCTAT GAGATCACCT 
GTGACTAGCC CAAATGGAAC CACTCCTTCG AATAAAACTT CTATAGTGAA ATCCTCTATG 
CCATCATCTT CTTCTAAGGC TTCACCAGCA AAGTCACCAT TGCGGAATGA TATGCCCTCT 
ACTGTTAAGG ATAGGAGCCA GTTACAGAAA GATCAGTCTG AATGTAAAAT TGAGCATGAG 
GATTCTGAGG ATGATAGACC TTTAAGTTCC ATACTATCTG GAAATAAAGG GCCAACCTCT 
TCGCGGCAGG TTTCTTCACC GCAGCCAGAG AAAAAGAATA ATGGTGATCG ACCTCTTGAT 
AGAGCAAGCA GAATTATAAA AGACGAGTCA GATGATGAAA CTCCAATCTC GTCAATGTTT 
CGGAAGAAGA TTGATAGTGG GATGTCAGGG GGCAACCAGC TCTCCAATGA TGAAAAGAAA 
CCTTTGGTTC AAAAGTTGCA TCAGAATGGT TCCACAGTTA AGAATGAAGT GCCGAATGGT 
AAGGTATTAG GTAAGAGACC TCTGGAGAAG AATAGTTCTG CAGATCAATC TTCCTTGAAG 
AAGGCCAAAA TATCAGCTTC ACCTACTTCA GTGAAAATGA AACAAGATTC TGTCAAGAAA 
GAGATAGATG ATAAGGGAAG GGTTTTGGTT TCACCAAAAA TGAAAGCTAA ACAGTTATCT 
ACCAGAGAAG ATGGAACTGA TGATGATGAT GATGATGATG TTCCGATATC CAAGAGATTC 
AAATCAGATT CCTCCAACAG TAACACATCA TCTGCAAAGC CAAAAGCAGT AAAACTAAAT 
TCTACTTCAT CAGCTGCAAA GCCAAAAGCG AGAAATGTCG TTTCTCCTAG GTCCAGAGCA 
ATGACCAAAA ACACTAAGAA AGTAACGAAG GACTCAAAAT ATTCCACGTC TTCAAAATCA 
TCACCTTCCT CTGGAGATGG GCAAAAGAAA TGGACTACTT TGGTGCACAA CGGTGTAATA 
TTTCCACCGC CATACAAGCC TCATGGCATT AAGATTTTGT ACAAGGGGAA ACCTGTTGAC 
CTAACTATTG AACAAGAAGA GGTTGCGACT ATGTTTGCAG TGATGAGAGA GACAGATTAT 
TATACTAAAC CTCAATTTCG GGAGAATTTC TGGAATGACT GGCGAAGACT GCTTGGCAAG 
AAACATGTGA TACAGAAGTT GGATGATTGT GACTTTACTC CCATATATGA GTGGCATTTG 
GAAGAGAAAG AGAAGAAGAA ACAAATGAGC ACAGAGGAGA AAAAAGCTTT GAAAGAGGAG 
AAAATGAAGC AAGAGGAGAA ATATATGTGG GCTGTTGTTG ATGGTGTCAA AGAGAAGATT 
GGTAATTTCA GAGTGGAACC GCCTGGCCTT TTCAGAGGCC GTGGAGAACA TCCCAAGATG 
GGAAAACTAA AGAAGCGTAT CCATCCATGC GAGATCACCT TAAATATCGG GAAAGGTGCT 
CCTATTCCAG AATGTCCAAT TGCTGGTGAA AGATGGAAAG AAGTAAAGCA TGATAACACA 
GTCACCTGGC TAGCTTTCTG GGCTGATCCT ATTAACCCAA AAGAATTCAA GTATGTATTT 
TTGGGAGCTG GCAGTTCTCT GAAAGGGCTG AGCGACAAAG AGAAGTACGA GAAAGCTAGG 
AATCTTACGG ATCATATAGA TAATATAAGA ACAACATACA CCAAGAATTT TACTGCCAAG 
GATGTTAAAA TGAGGCAAAT TGCTGTGGCG ACATATCTCA TTGATAAATT GGCGCTTAGG 
GCTGGAAATG AGAAGGATGA TGATGAGGCC GACACTGTTG GTTGTTGTAC TTTAAAAGTT 
GGAAATGTGG AGTGTATTCC TCCAAATAAA ATAAAGTTTG ATTTTCTTGG TAAGGATTCA 
ATCCAGTATG TGAACACAGT CGAGGTTGAG CCTCTTGTTT ACAAGGCCAT TGGACAGTTC 
CAGGCGGGAA AATCCAAAAC AGATGATCTT TTTGATGAGT TAGACACAAG TAAACTGAAT 
GCTCATTTGA AGGAACTTGT GCCTGGTCTC ACAGCTAAAG TATTCCGTAC ATATAATGCT 
TCCATCACTT TGGATGAAAT GCTGAGTCAA GAAACCAAAG ATGGCGATGT TACTCAAAAA 
ATAGTAGTTT ATCAGAAAGC AAATAAGGAG GTTGCCATAA TCTGCAATCA TCAGCGTACT 
GTATCAAAGA CACATGGTGC GCAAATTGAA AAATTGACTG CGAGAATAGA AGAACTCAAG 
GAGGTTCTTA AAGAGCTCAA AACTAATCTT GACCGGGCTA AAAAGGGAAA ACCACCATTG 
GAAGGTTCTG ATGGAAAGAA GATTAGAAGC TTAGAACCAA ACGCGTGGGA GAAGAAGATT 
GCTCAACAGA GTGCTAAGAT CGAGAAAATG GAACGAGACA TGCATACAAA AGAGGACTTG 
AAAACAGTGG CACTGGGCAC GTCTAAGATC AACTACCTGG ATCCTAGAAT CACAGTTGCA 
TGGTGCAAAC GTCATGAAGT ACCAATCGAA AAGATATTCA CCAAGTCTCT TTTGGAGAAG 
TTTGCGTGGG CAATGGATGT AGAACCCGAG TACAGATTCT CGCGCCGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200341.1
CDS	164..2914
Translation	MGTETVSKPVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMRSPVTSPNGTTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQLQKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNEVPNGKVLGKRPLEKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFTKSLLEKFAWAMDVEPEYRFSRR

Target ORF information:

RefSeq Version	NM_124912.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124912.5

ORF Insert Sequence:

ATGGGCACTG AAACAGTTTC AAAACCTGTG ATGGATAATG GGTCTGGAGA CAGTGATGAT 
GACAAGCCTT TAGCGTTCAA GAGGAATAAT ACAGTGGCTT CTAATTCGAA TCAATCTAAA 
TCCAATTCCC AGAGAAGCAA GGCAGTTCCT ACCACCAAGG TATCACCTAT GAGATCACCT 
GTGACTAGCC CAAATGGAAC CACTCCTTCG AATAAAACTT CTATAGTGAA ATCCTCTATG 
CCATCATCTT CTTCTAAGGC TTCACCAGCA AAGTCACCAT TGCGGAATGA TATGCCCTCT 
ACTGTTAAGG ATAGGAGCCA GTTACAGAAA GATCAGTCTG AATGTAAAAT TGAGCATGAG 
GATTCTGAGG ATGATAGACC TTTAAGTTCC ATACTATCTG GAAATAAAGG GCCAACCTCT 
TCGCGGCAGG TTTCTTCACC GCAGCCAGAG AAAAAGAATA ATGGTGATCG ACCTCTTGAT 
AGAGCAAGCA GAATTATAAA AGACGAGTCA GATGATGAAA CTCCAATCTC GTCAATGTTT 
CGGAAGAAGA TTGATAGTGG GATGTCAGGG GGCAACCAGC TCTCCAATGA TGAAAAGAAA 
CCTTTGGTTC AAAAGTTGCA TCAGAATGGT TCCACAGTTA AGAATGAAGT GCCGAATGGT 
AAGGTATTAG GTAAGAGACC TCTGGAGAAG AATAGTTCTG CAGATCAATC TTCCTTGAAG 
AAGGCCAAAA TATCAGCTTC ACCTACTTCA GTGAAAATGA AACAAGATTC TGTCAAGAAA 
GAGATAGATG ATAAGGGAAG GGTTTTGGTT TCACCAAAAA TGAAAGCTAA ACAGTTATCT 
ACCAGAGAAG ATGGAACTGA TGATGATGAT GATGATGATG TTCCGATATC CAAGAGATTC 
AAATCAGATT CCTCCAACAG TAACACATCA TCTGCAAAGC CAAAAGCAGT AAAACTAAAT 
TCTACTTCAT CAGCTGCAAA GCCAAAAGCG AGAAATGTCG TTTCTCCTAG GTCCAGAGCA 
ATGACCAAAA ACACTAAGAA AGTAACGAAG GACTCAAAAT ATTCCACGTC TTCAAAATCA 
TCACCTTCCT CTGGAGATGG GCAAAAGAAA TGGACTACTT TGGTGCACAA CGGTGTAATA 
TTTCCACCGC CATACAAGCC TCATGGCATT AAGATTTTGT ACAAGGGGAA ACCTGTTGAC 
CTAACTATTG AACAAGAAGA GGTTGCGACT ATGTTTGCAG TGATGAGAGA GACAGATTAT 
TATACTAAAC CTCAATTTCG GGAGAATTTC TGGAATGACT GGCGAAGACT GCTTGGCAAG 
AAACATGTGA TACAGAAGTT GGATGATTGT GACTTTACTC CCATATATGA GTGGCATTTG 
GAAGAGAAAG AGAAGAAGAA ACAAATGAGC ACAGAGGAGA AAAAAGCTTT GAAAGAGGAG 
AAAATGAAGC AAGAGGAGAA ATATATGTGG GCTGTTGTTG ATGGTGTCAA AGAGAAGATT 
GGTAATTTCA GAGTGGAACC GCCTGGCCTT TTCAGAGGCC GTGGAGAACA TCCCAAGATG 
GGAAAACTAA AGAAGCGTAT CCATCCATGC GAGATCACCT TAAATATCGG GAAAGGTGCT 
CCTATTCCAG AATGTCCAAT TGCTGGTGAA AGATGGAAAG AAGTAAAGCA TGATAACACA 
GTCACCTGGC TAGCTTTCTG GGCTGATCCT ATTAACCCAA AAGAATTCAA GTATGTATTT 
TTGGGAGCTG GCAGTTCTCT GAAAGGGCTG AGCGACAAAG AGAAGTACGA GAAAGCTAGG 
AATCTTACGG ATCATATAGA TAATATAAGA ACAACATACA CCAAGAATTT TACTGCCAAG 
GATGTTAAAA TGAGGCAAAT TGCTGTGGCG ACATATCTCA TTGATAAATT GGCGCTTAGG 
GCTGGAAATG AGAAGGATGA TGATGAGGCC GACACTGTTG GTTGTTGTAC TTTAAAAGTT 
GGAAATGTGG AGTGTATTCC TCCAAATAAA ATAAAGTTTG ATTTTCTTGG TAAGGATTCA 
ATCCAGTATG TGAACACAGT CGAGGTTGAG CCTCTTGTTT ACAAGGCCAT TGGACAGTTC 
CAGGCGGGAA AATCCAAAAC AGATGATCTT TTTGATGAGT TAGACACAAG TAAACTGAAT 
GCTCATTTGA AGGAACTTGT GCCTGGTCTC ACAGCTAAAG TATTCCGTAC ATATAATGCT 
TCCATCACTT TGGATGAAAT GCTGAGTCAA GAAACCAAAG ATGGCGATGT TACTCAAAAA 
ATAGTAGTTT ATCAGAAAGC AAATAAGGAG GTTGCCATAA TCTGCAATCA TCAGCGTACT 
GTATCAAAGA CACATGGTGC GCAAATTGAA AAATTGACTG CGAGAATAGA AGAACTCAAG 
GAGGTTCTTA AAGAGCTCAA AACTAATCTT GACCGGGCTA AAAAGGGAAA ACCACCATTG 
GAAGGTTCTG ATGGAAAGAA GATTAGAAGC TTAGAACCAA ACGCGTGGGA GAAGAAGATT 
GCTCAACAGA GTGCTAAGAT CGAGAAAATG GAACGAGACA TGCATACAAA AGAGGACTTG 
AAAACAGTGG CACTGGGCAC GTCTAAGATC AACTACCTGG ATCCTAGAAT CACAGTTGCA 
TGGTGCAAAC GTCATGAAGT ACCAATCGAA AAGATATTCA CCAAGTCTCT TTTGGAGAAG 
TTTGCGTGGG CAATGGATGT AGAACCCGAG TACAGATTCT CGCGCCGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP1ALPHA ( NM_001203614.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190543.1 Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.

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CloneID	OAb30963
Clone ID Related Accession (Same CDS sequence)	NM_001203614.1
Accession Version	NM_001203614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA topoisomerase I alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGGCACTG AAACAGTTTC AAAACCTGTG ATGGATAATG GGTCTGGAGA CAGTGATGAT 
GACAAGCCTT TAGCGTTCAA GAGGAATAAT ACAGTGGCTT CTAATTCGAA TCAATCTAAA 
TCCAATTCCC AGAGAAGCAA GGCAGTTCCT ACCACCAAGG TATCACCTAT GAGATCACCT 
GTGACTAGCC CAAATGGAAC CACTCCTTCG AATAAAACTT CTATAGTGAA ATCCTCTATG 
CCATCATCTT CTTCTAAGGC TTCACCAGCA AAGTCACCAT TGCGGAATGA TATGCCCTCT 
ACTGTTAAGG ATAGGAGCCA GTTACAGAAA GATCAGTCTG AATGTAAAAT TGAGCATGAG 
GATTCTGAGG ATGATAGACC TTTAAGTTCC ATACTATCTG GAAATAAAGG GCCAACCTCT 
TCGCGGCAGG TTTCTTCACC GCAGCCAGAG AAAAAGAATA ATGGTGATCG ACCTCTTGAT 
AGAGCAAGCA GAATTATAAA AGACGAGTCA GATGATGAAA CTCCAATCTC GTCAATGTTT 
CGGAAGAAGA TTGATAGTGG GATGTCAGGG GGCAACCAGC TCTCCAATGA TGAAAAGAAA 
CCTTTGGTTC AAAAGTTGCA TCAGAATGGT TCCACAGTTA AGAATGAAGT GCCGAATGGT 
AAGGTATTAG GTAAGAGACC TCTGGAGAAG AATAGTTCTG CAGATCAATC TTCCTTGAAG 
AAGGCCAAAA TATCAGCTTC ACCTACTTCA GTGAAAATGA AACAAGATTC TGTCAAGAAA 
GAGATAGATG ATAAGGGAAG GGTTTTGGTT TCACCAAAAA TGAAAGCTAA ACAGTTATCT 
ACCAGAGAAG ATGGAACTGA TGATGATGAT GATGATGATG TTCCGATATC CAAGAGATTC 
AAATCAGATT CCTCCAACAG TAACACATCA TCTGCAAAGC CAAAAGCAGT AAAACTAAAT 
TCTACTTCAT CAGCTGCAAA GCCAAAAGCG AGAAATGTCG TTTCTCCTAG GTCCAGAGCA 
ATGACCAAAA ACACTAAGAA AGTAACGAAG GACTCAAAAT ATTCCACGTC TTCAAAATCA 
TCACCTTCCT CTGGAGATGG GCAAAAGAAA TGGACTACTT TGGTGCACAA CGGTGTAATA 
TTTCCACCGC CATACAAGCC TCATGGCATT AAGATTTTGT ACAAGGGGAA ACCTGTTGAC 
CTAACTATTG AACAAGAAGA GGTTGCGACT ATGTTTGCAG TGATGAGAGA GACAGATTAT 
TATACTAAAC CTCAATTTCG GGAGAATTTC TGGAATGACT GGCGAAGACT GCTTGGCAAG 
AAACATGTGA TACAGAAGTT GGATGATTGT GACTTTACTC CCATATATGA GTGGCATTTG 
GAAGAGAAAG AGAAGAAGAA ACAAATGAGC ACAGAGGAGA AAAAAGCTTT GAAAGAGGAG 
AAAATGAAGC AAGAGGAGAA ATATATGTGG GCTGTTGTTG ATGGTGTCAA AGAGAAGATT 
GGTAATTTCA GAGTGGAACC GCCTGGCCTT TTCAGAGGCC GTGGAGAACA TCCCAAGATG 
GGAAAACTAA AGAAGCGTAT CCATCCATGC GAGATCACCT TAAATATCGG GAAAGGTGCT 
CCTATTCCAG AATGTCCAAT TGCTGGTGAA AGATGGAAAG AAGTAAAGCA TGATAACACA 
GTCACCTGGC TAGCTTTCTG GGCTGATCCT ATTAACCCAA AAGAATTCAA GTATGTATTT 
TTGGGAGCTG GCAGTTCTCT GAAAGGGCTG AGCGACAAAG AGAAGTACGA GAAAGCTAGG 
AATCTTACGG ATCATATAGA TAATATAAGA ACAACATACA CCAAGAATTT TACTGCCAAG 
GATGTTAAAA TGAGGCAAAT TGCTGTGGCG ACATATCTCA TTGATAAATT GGCGCTTAGG 
GCTGGAAATG AGAAGGATGA TGATGAGGCC GACACTGTTG GTTGTTGTAC TTTAAAAGTT 
GGAAATGTGG AGTGTATTCC TCCAAATAAA ATAAAGTTTG ATTTTCTTGG TAAGGATTCA 
ATCCAGTATG TGAACACAGT CGAGGTTGAG CCTCTTGTTT ACAAGGCCAT TGGACAGTTC 
CAGGCGGGAA AATCCAAAAC AGATGATCTT TTTGATGAGT TAGACACAAG TAAACTGAAT 
GCTCATTTGA AGGAACTTGT GCCTGGTCTC ACAGCTAAAG TATTCCGTAC ATATAATGCT 
TCCATCACTT TGGATGAAAT GCTGAGTCAA GAAACCAAAG ATGGCGATGT TACTCAAAAA 
ATAGTAGTTT ATCAGAAAGC AAATAAGGAG GTTGCCATAA TCTGCAATCA TCAGCGTACT 
GTATCAAAGA CACATGGTGC GCAAATTGAA AAATTGACTG CGAGAATAGA AGAACTCAAG 
GAGGTTCTTA AAGAGCTCAA AACTAATCTT GACCGGGCTA AAAAGGGAAA ACCACCATTG 
GAAGGTTCTG ATGGAAAGAA GATTAGAAGC TTAGAACCAA ACGCGTGGGA GAAGAAGATT 
GCTCAACAGA GTGCTAAGAT CGAGAAAATG GAACGAGACA TGCATACAAA AGAGGACTTG 
AAAACAGTGG CACTGGGCAC GTCTAAGATC AACTACCTGG ATCCTAGAAT CACAGTTGCA 
TGGTGCAAAC GTCATGAAGT ACCAATCGAA AAGATATTCA CCAAGTCTCT TTTGGAGAAG 
TTTGCGTGGG CAATGGATGT AGAACCCGAA GCAAAGAGAA AACCGGCCTT TCTTCTATTC 
ATCTTCCTCC CTTGTTTAGA ATTTTATTAC CTTAGTGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190543.1
CDS	152..2953
Translation	MGTETVSKPVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMRSPVTSPNGTTPSNKTSIVKSSMPSSSSKASPAKSPLRNDMPSTVKDRSQLQKDQSECKIEHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNEVPNGKVLGKRPLEKNSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFTKSLLEKFAWAMDVEPEAKRKPAFLLFIFLPCLEFYYLSE

Target ORF information:

RefSeq Version	NM_001203614.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA topoisomerase I alpha (TOP1ALPHA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203614.1

ORF Insert Sequence:

ATGGGCACTG AAACAGTTTC AAAACCTGTG ATGGATAATG GGTCTGGAGA CAGTGATGAT 
GACAAGCCTT TAGCGTTCAA GAGGAATAAT ACAGTGGCTT CTAATTCGAA TCAATCTAAA 
TCCAATTCCC AGAGAAGCAA GGCAGTTCCT ACCACCAAGG TATCACCTAT GAGATCACCT 
GTGACTAGCC CAAATGGAAC CACTCCTTCG AATAAAACTT CTATAGTGAA ATCCTCTATG 
CCATCATCTT CTTCTAAGGC TTCACCAGCA AAGTCACCAT TGCGGAATGA TATGCCCTCT 
ACTGTTAAGG ATAGGAGCCA GTTACAGAAA GATCAGTCTG AATGTAAAAT TGAGCATGAG 
GATTCTGAGG ATGATAGACC TTTAAGTTCC ATACTATCTG GAAATAAAGG GCCAACCTCT 
TCGCGGCAGG TTTCTTCACC GCAGCCAGAG AAAAAGAATA ATGGTGATCG ACCTCTTGAT 
AGAGCAAGCA GAATTATAAA AGACGAGTCA GATGATGAAA CTCCAATCTC GTCAATGTTT 
CGGAAGAAGA TTGATAGTGG GATGTCAGGG GGCAACCAGC TCTCCAATGA TGAAAAGAAA 
CCTTTGGTTC AAAAGTTGCA TCAGAATGGT TCCACAGTTA AGAATGAAGT GCCGAATGGT 
AAGGTATTAG GTAAGAGACC TCTGGAGAAG AATAGTTCTG CAGATCAATC TTCCTTGAAG 
AAGGCCAAAA TATCAGCTTC ACCTACTTCA GTGAAAATGA AACAAGATTC TGTCAAGAAA 
GAGATAGATG ATAAGGGAAG GGTTTTGGTT TCACCAAAAA TGAAAGCTAA ACAGTTATCT 
ACCAGAGAAG ATGGAACTGA TGATGATGAT GATGATGATG TTCCGATATC CAAGAGATTC 
AAATCAGATT CCTCCAACAG TAACACATCA TCTGCAAAGC CAAAAGCAGT AAAACTAAAT 
TCTACTTCAT CAGCTGCAAA GCCAAAAGCG AGAAATGTCG TTTCTCCTAG GTCCAGAGCA 
ATGACCAAAA ACACTAAGAA AGTAACGAAG GACTCAAAAT ATTCCACGTC TTCAAAATCA 
TCACCTTCCT CTGGAGATGG GCAAAAGAAA TGGACTACTT TGGTGCACAA CGGTGTAATA 
TTTCCACCGC CATACAAGCC TCATGGCATT AAGATTTTGT ACAAGGGGAA ACCTGTTGAC 
CTAACTATTG AACAAGAAGA GGTTGCGACT ATGTTTGCAG TGATGAGAGA GACAGATTAT 
TATACTAAAC CTCAATTTCG GGAGAATTTC TGGAATGACT GGCGAAGACT GCTTGGCAAG 
AAACATGTGA TACAGAAGTT GGATGATTGT GACTTTACTC CCATATATGA GTGGCATTTG 
GAAGAGAAAG AGAAGAAGAA ACAAATGAGC ACAGAGGAGA AAAAAGCTTT GAAAGAGGAG 
AAAATGAAGC AAGAGGAGAA ATATATGTGG GCTGTTGTTG ATGGTGTCAA AGAGAAGATT 
GGTAATTTCA GAGTGGAACC GCCTGGCCTT TTCAGAGGCC GTGGAGAACA TCCCAAGATG 
GGAAAACTAA AGAAGCGTAT CCATCCATGC GAGATCACCT TAAATATCGG GAAAGGTGCT 
CCTATTCCAG AATGTCCAAT TGCTGGTGAA AGATGGAAAG AAGTAAAGCA TGATAACACA 
GTCACCTGGC TAGCTTTCTG GGCTGATCCT ATTAACCCAA AAGAATTCAA GTATGTATTT 
TTGGGAGCTG GCAGTTCTCT GAAAGGGCTG AGCGACAAAG AGAAGTACGA GAAAGCTAGG 
AATCTTACGG ATCATATAGA TAATATAAGA ACAACATACA CCAAGAATTT TACTGCCAAG 
GATGTTAAAA TGAGGCAAAT TGCTGTGGCG ACATATCTCA TTGATAAATT GGCGCTTAGG 
GCTGGAAATG AGAAGGATGA TGATGAGGCC GACACTGTTG GTTGTTGTAC TTTAAAAGTT 
GGAAATGTGG AGTGTATTCC TCCAAATAAA ATAAAGTTTG ATTTTCTTGG TAAGGATTCA 
ATCCAGTATG TGAACACAGT CGAGGTTGAG CCTCTTGTTT ACAAGGCCAT TGGACAGTTC 
CAGGCGGGAA AATCCAAAAC AGATGATCTT TTTGATGAGT TAGACACAAG TAAACTGAAT 
GCTCATTTGA AGGAACTTGT GCCTGGTCTC ACAGCTAAAG TATTCCGTAC ATATAATGCT 
TCCATCACTT TGGATGAAAT GCTGAGTCAA GAAACCAAAG ATGGCGATGT TACTCAAAAA 
ATAGTAGTTT ATCAGAAAGC AAATAAGGAG GTTGCCATAA TCTGCAATCA TCAGCGTACT 
GTATCAAAGA CACATGGTGC GCAAATTGAA AAATTGACTG CGAGAATAGA AGAACTCAAG 
GAGGTTCTTA AAGAGCTCAA AACTAATCTT GACCGGGCTA AAAAGGGAAA ACCACCATTG 
GAAGGTTCTG ATGGAAAGAA GATTAGAAGC TTAGAACCAA ACGCGTGGGA GAAGAAGATT 
GCTCAACAGA GTGCTAAGAT CGAGAAAATG GAACGAGACA TGCATACAAA AGAGGACTTG 
AAAACAGTGG CACTGGGCAC GTCTAAGATC AACTACCTGG ATCCTAGAAT CACAGTTGCA 
TGGTGCAAAC GTCATGAAGT ACCAATCGAA AAGATATTCA CCAAGTCTCT TTTGGAGAAG 
TTTGCGTGGG CAATGGATGT AGAACCCGAA GCAAAGAGAA AACCGGCCTT TCTTCTATTC 
ATCTTCCTCC CTTGTTTAGA ATTTTATTAC CTTAGTGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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TOP1ALPHA cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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