LON2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	LON2
Entrez Gene ID	834750
Full Name	lon protease 2
Synonyms	ABERRANT PEROXISOME MORPHOLOGY 10,APEM10,MQD22.18,MQD22_18,lon protease 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_124075.4	NP_568675.1	lon protease 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Lonp2	NP_080103.1
Arabidopsis thaliana (thale cress)	LON2	NP_568675.1
Oryza sativa (rice)	Os09g0533400	NP_001063769.1
Bos taurus (cattle)	LONP2	NP_001029895.1
Homo sapiens (human)	LONP2	NP_113678.2
Canis lupus familiaris (dog)	LONP2	XP_003638980.1
Xenopus tropicalis (tropical clawed frog)	lonp2	NP_001120534.1
Pan troglodytes (chimpanzee)	LONP2	XP_520624.3
Gallus gallus (chicken)	LONP2	NP_001186149.1
Danio rerio (zebrafish)	lonp2	NP_001008573.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified the role of LON2 protease and the photorespiratory enzyme HPR1 in drought resistance.
Title: Using Co-Expression Analysis and Stress-Based Screens to Uncover Arabidopsis Peroxisomal Proteins Involved in Drought Response.

pexophagy is accelerated when the LON2 protease is disabled
Title: Mutation of the Arabidopsis LON2 peroxisomal protease enhances pexophagy.

LON2- and autophagy-dependent pathways are interdependent, with the chaperone function of LON2 suppressing autophagic peroxisome degradation
Title: Interaction between chaperone and protease functions of LON2, and autophagy during the functional transition of peroxisomes.

atg2, atg3, and atg7 mutations suppressed lon2 defects in auxin metabolism and matrix protein processing and rescued the abnormally large size and small number of lon2 peroxisomes
Title: Disrupting autophagy restores peroxisome function to an Arabidopsis lon2 mutant and reveals a role for the LON2 protease in peroxisomal matrix protein degradation.

LON2 is necessary for IBA-stimulated lateral root formation and for sustained matrix protein import into peroxisomes.
Title: Arabidopsis LON2 is necessary for peroxisomal function and sustained matrix protein import.

The following LON2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LON2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb20788	NM_124075.4 Latest version!	Arabidopsis thaliana lon protease 2 (LON2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LON2 ( NM_124075.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_568675.1 Arabidopsis thaliana lon protease 2 (LON2), mRNA.

Price & Availability↑

CloneID	OAb20788
Clone ID Related Accession (Same CDS sequence)	NM_124075.4
Accession Version	NM_124075.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	lon protease 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_124075.3.

ATGGCGGAAA CAGTGGAGCT CCCGAGTAGG TTAGCGATTC TTCCTTTCAG GAATAAGGTT 
CTCTTACCTG GCGCCATTAT TCGAATCCGT TGTACTTCTC ATAGCAGCGT CACACTAGTG 
GAGCAAGAGC TATGGCAGAA AGAAGAGAAA GGTTTAATTG GCATTTTACC TGTTCGTGAT 
GATGCCGAGG GTTCTTCAAT TGGGACGATG ATTAATCCAG GTGCTGGAAG TGATTCTGGA 
GAAAGAAGCT TGAAATTTCT AGTTGGTACA ACAGATGCTC AGAAGTCTGA TGCTAAAGAT 
CAGCAGGATC TTCAATGGCA CACCAGGGGA GTAGCAGCTC GTGCTCTGCA TCTTTCAAGA 
GGGGTGGAAA AACCAAGTGG AAGAGTTACG TATGTGGTTG TCCTTGAAGG TCTGAGCAGG 
TTTAATGTTC AGGAGCTTGG AAAGAGAGGA CCATATTCGG TTGCTCGGAT TACATCGCTC 
GAGATGACAA AGGCAGAGTT GGAGCAAGTA AAACAAGATC CAGATTTTGT AGCACTGTCA 
CGCCAGTTTA AAACCACAGC TATGGAGCTT GTCTCTGTGC TTGAGCAGAA ACAAAAAACT 
GGTGGAAGGA CAAAAGTGCT TTTGGAGACA GTTCCTATCC ATAAACTAGC AGACATATTT 
GTTGCTAGCT TTGAGATGAG TTTTGAAGAG CAACTATCTA TGCTGGATTC AGTTGACCTT 
AAAGTAAGAC TTTCAAAGGC TACTGAACTA GTTGATCGGC ATCTGCAGTC AATTCGCGTG 
GCAGAGAAGA TTACGCAGAA GGTGGAGGGC CAGTTGTCAA AGTCTCAGAA GGAGTATCTC 
TTGCGCCAGC AGATGAGAGC TATTAAAGAG GAGCTTGGTG ACAACGATGA TGATGAAGAT 
GATGTAGCTG CACTTGAAAG AAAGATGCAA GCTGCGGGAA TGCCGTCAAA CATATGGAAG 
CATGCTCAAA GGGAATTGAG GCGTCTAAAA AAGATGCAAC CTCAGCAACC TGGCTATAAC 
AGTTCACGAG TTTACCTGGA GCTGTTGGCT GATCTTCCTT GGGATAAGGC AAGCGAAGAA 
CATGAGTTGG ATCTAAAAGC AGCAAAAGAA CGTCTTGACA GTGATCACTA TGGTTTAGCC 
AAGGTCAAGC AACGGATCAT TGAATATCTG GCTGTTCGAA AGCTTAAACC AGATGCGAGA 
GGCCCAGTAT TGTGCTTTGT TGGTCCACCA GGTGTGGGCA AGACGTCTTT GGCATCATCT 
ATTGCAGCTG CATTAGGTAG AAAATTTGTT CGCTTATCTT TGGGTGGTGT GAAAGATGAA 
GCTGACATTA GAGGACACAG AAGAACTTAT ATAGGAAGCA TGCCAGGGCG CCTCATTGAT 
GGCCTAAAGA GGGTTGGTGT TTGCAATCCA GTTATGCTGT TGGATGAGAT TGACAAAACA 
GGCTCAGACG TTCGGGGTGA TCCAGCTTCT GCACTGCTCG AGGTTTTGGA TCCAGAGCAG 
AACAAATCTT TTAATGATCA CTATCTGAAT GTTCCATATG ACTTATCAAA GGTGGTTTTT 
GTTGCCACTG CTAATAGGGT TCAACCTATT CCACCTCCTC TTCTAGACAG GATGGAGCTA 
ATTGAGTTGC CTGGTTATAC GCAAGAGGAA AAACTTAAGA TAGCTATGCG CCATCTGATT 
CCTCGAGTTC TAGATCAGCA TGGGCTTAGT TCCGAGTTCC TCAAGATTCC AGAGGCTATG 
GTAAAGAATA TAATTCAGAG GTATACAAGG GAAGCCGGTG TTCGTAGTCT AGAGAGGAAC 
TTGGCTGCTT TAGCTCGTGC AGCTGCTGTG ATGGTGGCAG AGCATGAACA AAGTCTTCCG 
TTGAGCAAAG ATGTGCAGAA ACTTACATCT CCTCTGCTTA ATGGTAGAAT GGCCGAAGGA 
GGCGAAGTGG AAATGGAAGT TATTCCAATG GGTGTAAATG ATCATGAGAT TGGAGGCACC 
TTCCAGAGTC CCTCAGCTTT GGTGGTGGAT GAGACCATGC TTGAAAAAAT CCTCGGGCCT 
CCAAGGTTTG ATGACAGTGA AGCCGCTGAT CGAGTGGCGT CAGCTGGCGT TTCAGTGGGT 
CTCGTTTGGA CTACTTTTGG TGGTGAAGTC CAGTTCGTGG AGGCTACATC TATGGTGGGC 
AAAGGCGAAA TGCATTTGAC GGGCCAACTC GGAGATGTTA TCAAAGAATC TGCTCAATTA 
GCTCTTACAT GGGTAAGAGC TCGTGCGTCG GATTTTAAGC TGGCACTTGC AGGGGATATG 
AATGTTCTTG ATGGACGAGA CATCCACATT CACTTTCCTG CTGGTGCAGT GCCGAAAGAC 
GGGCCTTCTG CGGGAGTGAC TTTGGTGACA GCTTTGGTTT CGTTGTTCAG TCAGAAACGA 
GTTAGAGCAG ACACAGCCAT GACCGGAGAG ATGACACTCA GAGGTCTCGT TCTGCCTGTT 
GGTGGCATCA AAGATAAGAT ATTGGCTGCA CACCGGTATG GTATCAAAAG AGTGATTCTA 
CCGCAAAGGA ACTCGAAAGA CTTAGTTGAA GTACCCGCCG CTGTACTTTC CAGTCTGGAG 
GTGATATTGG CAAAGAGAAT GGAAGACGTC CTGGAAAATG CATTCGAAGG AGGTTGTCCA 
TGGCGGAACA ACTACTCTAA ATTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_568675.1
CDS	95..2761
Translation	MAETVELPSRLAILPFRNKVLLPGAIIRIRCTSHSSVTLVEQELWQKEEKGLIGILPVRDDAEGSSIGTMINPGAGSDSGERSLKFLVGTTDAQKSDAKDQQDLQWHTRGVAARALHLSRGVEKPSGRVTYVVVLEGLSRFNVQELGKRGPYSVARITSLEMTKAELEQVKQDPDFVALSRQFKTTAMELVSVLEQKQKTGGRTKVLLETVPIHKLADIFVASFEMSFEEQLSMLDSVDLKVRLSKATELVDRHLQSIRVAEKITQKVEGQLSKSQKEYLLRQQMRAIKEELGDNDDDEDDVAALERKMQAAGMPSNIWKHAQRELRRLKKMQPQQPGYNSSRVYLELLADLPWDKASEEHELDLKAAKERLDSDHYGLAKVKQRIIEYLAVRKLKPDARGPVLCFVGPPGVGKTSLASSIAAALGRKFVRLSLGGVKDEADIRGHRRTYIGSMPGRLIDGLKRVGVCNPVMLLDEIDKTGSDVRGDPASALLEVLDPEQNKSFNDHYLNVPYDLSKVVFVATANRVQPIPPPLLDRMELIELPGYTQEEKLKIAMRHLIPRVLDQHGLSSEFLKIPEAMVKNIIQRYTREAGVRSLERNLAALARAAAVMVAEHEQSLPLSKDVQKLTSPLLNGRMAEGGEVEMEVIPMGVNDHEIGGTFQSPSALVVDETMLEKILGPPRFDDSEAADRVASAGVSVGLVWTTFGGEVQFVEATSMVGKGEMHLTGQLGDVIKESAQLALTWVRARASDFKLALAGDMNVLDGRDIHIHFPAGAVPKDGPSAGVTLVTALVSLFSQKRVRADTAMTGEMTLRGLVLPVGGIKDKILAAHRYGIKRVILPQRNSKDLVEVPAAVLSSLEVILAKRMEDVLENAFEGGCPWRNNYSKL

Target ORF information:

RefSeq Version	NM_124075.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana lon protease 2 (LON2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124075.4

ORF Insert Sequence:

ATGGCGGAAA CAGTGGAGCT CCCGAGTAGG TTAGCGATTC TTCCTTTCAG GAATAAGGTT 
CTCTTACCTG GCGCCATTAT TCGAATCCGT TGTACTTCTC ATAGCAGCGT CACACTAGTG 
GAGCAAGAGC TATGGCAGAA AGAAGAGAAA GGTTTAATTG GCATTTTACC TGTTCGTGAT 
GATGCCGAGG GTTCTTCAAT TGGGACGATG ATTAATCCAG GTGCTGGAAG TGATTCTGGA 
GAAAGAAGCT TGAAATTTCT AGTTGGTACA ACAGATGCTC AGAAGTCTGA TGCTAAAGAT 
CAGCAGGATC TTCAATGGCA CACCAGGGGA GTAGCAGCTC GTGCTCTGCA TCTTTCAAGA 
GGGGTGGAAA AACCAAGTGG AAGAGTTACG TATGTGGTTG TCCTTGAAGG TCTGAGCAGG 
TTTAATGTTC AGGAGCTTGG AAAGAGAGGA CCATATTCGG TTGCTCGGAT TACATCGCTC 
GAGATGACAA AGGCAGAGTT GGAGCAAGTA AAACAAGATC CAGATTTTGT AGCACTGTCA 
CGCCAGTTTA AAACCACAGC TATGGAGCTT GTCTCTGTGC TTGAGCAGAA ACAAAAAACT 
GGTGGAAGGA CAAAAGTGCT TTTGGAGACA GTTCCTATCC ATAAACTAGC AGACATATTT 
GTTGCTAGCT TTGAGATGAG TTTTGAAGAG CAACTATCTA TGCTGGATTC AGTTGACCTT 
AAAGTAAGAC TTTCAAAGGC TACTGAACTA GTTGATCGGC ATCTGCAGTC AATTCGCGTG 
GCAGAGAAGA TTACGCAGAA GGTGGAGGGC CAGTTGTCAA AGTCTCAGAA GGAGTATCTC 
TTGCGCCAGC AGATGAGAGC TATTAAAGAG GAGCTTGGTG ACAACGATGA TGATGAAGAT 
GATGTAGCTG CACTTGAAAG AAAGATGCAA GCTGCGGGAA TGCCGTCAAA CATATGGAAG 
CATGCTCAAA GGGAATTGAG GCGTCTAAAA AAGATGCAAC CTCAGCAACC TGGCTATAAC 
AGTTCACGAG TTTACCTGGA GCTGTTGGCT GATCTTCCTT GGGATAAGGC AAGCGAAGAA 
CATGAGTTGG ATCTAAAAGC AGCAAAAGAA CGTCTTGACA GTGATCACTA TGGTTTAGCC 
AAGGTCAAGC AACGGATCAT TGAATATCTG GCTGTTCGAA AGCTTAAACC AGATGCGAGA 
GGCCCAGTAT TGTGCTTTGT TGGTCCACCA GGTGTGGGCA AGACGTCTTT GGCATCATCT 
ATTGCAGCTG CATTAGGTAG AAAATTTGTT CGCTTATCTT TGGGTGGTGT GAAAGATGAA 
GCTGACATTA GAGGACACAG AAGAACTTAT ATAGGAAGCA TGCCAGGGCG CCTCATTGAT 
GGCCTAAAGA GGGTTGGTGT TTGCAATCCA GTTATGCTGT TGGATGAGAT TGACAAAACA 
GGCTCAGACG TTCGGGGTGA TCCAGCTTCT GCACTGCTCG AGGTTTTGGA TCCAGAGCAG 
AACAAATCTT TTAATGATCA CTATCTGAAT GTTCCATATG ACTTATCAAA GGTGGTTTTT 
GTTGCCACTG CTAATAGGGT TCAACCTATT CCACCTCCTC TTCTAGACAG GATGGAGCTA 
ATTGAGTTGC CTGGTTATAC GCAAGAGGAA AAACTTAAGA TAGCTATGCG CCATCTGATT 
CCTCGAGTTC TAGATCAGCA TGGGCTTAGT TCCGAGTTCC TCAAGATTCC AGAGGCTATG 
GTAAAGAATA TAATTCAGAG GTATACAAGG GAAGCCGGTG TTCGTAGTCT AGAGAGGAAC 
TTGGCTGCTT TAGCTCGTGC AGCTGCTGTG ATGGTGGCAG AGCATGAACA AAGTCTTCCG 
TTGAGCAAAG ATGTGCAGAA ACTTACATCT CCTCTGCTTA ATGGTAGAAT GGCCGAAGGA 
GGCGAAGTGG AAATGGAAGT TATTCCAATG GGTGTAAATG ATCATGAGAT TGGAGGCACC 
TTCCAGAGTC CCTCAGCTTT GGTGGTGGAT GAGACCATGC TTGAAAAAAT CCTCGGGCCT 
CCAAGGTTTG ATGACAGTGA AGCCGCTGAT CGAGTGGCGT CAGCTGGCGT TTCAGTGGGT 
CTCGTTTGGA CTACTTTTGG TGGTGAAGTC CAGTTCGTGG AGGCTACATC TATGGTGGGC 
AAAGGCGAAA TGCATTTGAC GGGCCAACTC GGAGATGTTA TCAAAGAATC TGCTCAATTA 
GCTCTTACAT GGGTAAGAGC TCGTGCGTCG GATTTTAAGC TGGCACTTGC AGGGGATATG 
AATGTTCTTG ATGGACGAGA CATCCACATT CACTTTCCTG CTGGTGCAGT GCCGAAAGAC 
GGGCCTTCTG CGGGAGTGAC TTTGGTGACA GCTTTGGTTT CGTTGTTCAG TCAGAAACGA 
GTTAGAGCAG ACACAGCCAT GACCGGAGAG ATGACACTCA GAGGTCTCGT TCTGCCTGTT 
GGTGGCATCA AAGATAAGAT ATTGGCTGCA CACCGGTATG GTATCAAAAG AGTGATTCTA 
CCGCAAAGGA ACTCGAAAGA CTTAGTTGAA GTACCCGCCG CTGTACTTTC CAGTCTGGAG 
GTGATATTGG CAAAGAGAAT GGAAGACGTC CTGGAAAATG CATTCGAAGG AGGTTGTCCA 
TGGCGGAACA ACTACTCTAA ATTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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LON2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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