RRS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	RRS1
Entrez Gene ID	834562
Full Name	Disease resistance protein (TIR-NBS-LRR class)
Synonyms	ARABIDOPSIS THALIANA WRKY DOMAIN PROTEIN 52,ATWRKY52,K9E15.2,K9E15_2,RESISTANT TO RALSTONIA SOLANACEARUM 1,SENSITIVE TO LOW HUMIDITY 1,SLH1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001344604.1	NP_001318742.1	Disease resistance protein (TIR-NBS-LRR class)
NM_123894.4	NP_199339.2	Disease resistance protein (TIR-NBS-LRR class)
NM_001085246.2	NP_001078715.1	Disease resistance protein (TIR-NBS-LRR class)

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT5G38344	NP_001031980.1
Arabidopsis thaliana (thale cress)	WRR4	NP_176043.1
Arabidopsis thaliana (thale cress)	AT3G51570	NP_190725.1
Arabidopsis thaliana (thale cress)	AT4G04110	NP_192320.1
Arabidopsis thaliana (thale cress)	AT3G04210	NP_566223.1
Arabidopsis thaliana (thale cress)	AT5G18370	NP_197338.1
Arabidopsis thaliana (thale cress)	AT5G38350	NP_198651.1
Arabidopsis thaliana (thale cress)	AT5G38850	NP_198701.1
Arabidopsis thaliana (thale cress)	AT5G41540	NP_198969.1
Arabidopsis thaliana (thale cress)	RLM1	NP_176590.2
Arabidopsis thaliana (thale cress)	AT5G44870	NP_199300.1
Arabidopsis thaliana (thale cress)	AT5G44900	NP_199303.2
Arabidopsis thaliana (thale cress)	AT5G45060	NP_199319.1
Arabidopsis thaliana (thale cress)	AT5G46520	NP_199464.2
Arabidopsis thaliana (thale cress)	AT5G58120	NP_200620.1
Arabidopsis thaliana (thale cress)	AT5G48780	NP_199689.1
Arabidopsis thaliana (thale cress)	AT1G66090	NP_176783.1
Arabidopsis thaliana (thale cress)	AT1G09665	NP_683292.1
Arabidopsis thaliana (thale cress)	AT1G72860	NP_177429.2
Arabidopsis thaliana (thale cress)	AT1G72940	NP_177437.1
Arabidopsis thaliana (thale cress)	AT1G72910	NP_177434.1
Arabidopsis thaliana (thale cress)	TIR	NP_001077816.1
Arabidopsis thaliana (thale cress)	RPP4	NP_193420.2
Arabidopsis thaliana (thale cress)	AT4G16920	NP_193425.1
Arabidopsis thaliana (thale cress)	AT4G19510	NP_193686.5
Arabidopsis thaliana (thale cress)	AT2G20142	NP_973487.2
Arabidopsis thaliana (thale cress)	AT1G56540	NP_176047.1
Arabidopsis thaliana (thale cress)	AT1G63860	NP_001154443.1
Arabidopsis thaliana (thale cress)	AT4G36140	NP_195337.1
Arabidopsis thaliana (thale cress)	RPS4	NP_199338.1
Arabidopsis thaliana (thale cress)	AT1G31540	NP_001117396.1
Arabidopsis thaliana (thale cress)	AT5G46260	NP_199438.1
Arabidopsis thaliana (thale cress)	AT2G14080	NP_179024.1
Arabidopsis thaliana (thale cress)	AT5G18350	NP_197336.4
Arabidopsis thaliana (thale cress)	AT4G12010	NP_192938.1
Arabidopsis thaliana (thale cress)	RRS1	NP_001078715.1
Arabidopsis thaliana (thale cress)	AT5G46450	NP_199457.1
Arabidopsis thaliana (thale cress)	AT5G41550	NP_198970.1
Arabidopsis thaliana (thale cress)	PP2-A8	NP_199320.1
Arabidopsis thaliana (thale cress)	AT1G57830	NP_176093.1
Arabidopsis thaliana (thale cress)	AT4G19530	NP_193688.1
Arabidopsis thaliana (thale cress)	AT4G19520	NP_193687.3
Arabidopsis thaliana (thale cress)	AT5G46490	NP_974894.1
Arabidopsis thaliana (thale cress)	AT5G46510	NP_199463.1
Arabidopsis thaliana (thale cress)	AT1G60320	NP_176236.1
Arabidopsis thaliana (thale cress)	AT4G23515	NP_974598.1
Arabidopsis thaliana (thale cress)	AT4G19925	NP_974578.1
Arabidopsis thaliana (thale cress)	AT1G72950	NP_177438.1
Arabidopsis thaliana (thale cress)	SNC1	NP_193422.1
Arabidopsis thaliana (thale cress)	WRKY19	NP_001118968.1
Arabidopsis thaliana (thale cress)	AT1G63870	NP_176571.1
Arabidopsis thaliana (thale cress)	CSA1	NP_197290.1
Arabidopsis thaliana (thale cress)	AT1G69550	NP_564971.2
Arabidopsis thaliana (thale cress)	AT5G17680	NP_197270.1
Arabidopsis thaliana (thale cress)	AT5G17970	NP_197298.1
Arabidopsis thaliana (thale cress)	RPS6	NP_199459.2
Arabidopsis thaliana (thale cress)	DAR4	NP_197291.2
Arabidopsis thaliana (thale cress)	AT5G38340	NP_198650.1
Arabidopsis thaliana (thale cress)	AT5G40060	NP_198822.3
Arabidopsis thaliana (thale cress)	AT5G40100	NP_198826.1
Arabidopsis thaliana (thale cress)	AT1G17600	NP_173203.2
Arabidopsis thaliana (thale cress)	AT5G40910	NP_198907.1
Arabidopsis thaliana (thale cress)	AT5G41740	NP_001154757.1
Arabidopsis thaliana (thale cress)	PP2-A5	NP_176718.2
Arabidopsis thaliana (thale cress)	AT5G45000	NP_199313.3
Arabidopsis thaliana (thale cress)	AT5G45200	NP_199333.1
Arabidopsis thaliana (thale cress)	AT2G17060	NP_179298.2
Arabidopsis thaliana (thale cress)	AT5G45210	NP_199334.1
Arabidopsis thaliana (thale cress)	AT5G46270	NP_199439.5
Arabidopsis thaliana (thale cress)	AT4G08450	NP_192585.1
Arabidopsis thaliana (thale cress)	AT4G11170	NP_192855.1
Arabidopsis thaliana (thale cress)	AT1G56520	NP_176044.1
Arabidopsis thaliana (thale cress)	AT1G63740	NP_176561.2
Arabidopsis thaliana (thale cress)	AT1G57850	NP_176094.1
Arabidopsis thaliana (thale cress)	AT4G11340	NP_192873.1
Arabidopsis thaliana (thale cress)	AT4G09430	NP_192681.1
Arabidopsis thaliana (thale cress)	AT1G72920	NP_177435.1
Arabidopsis thaliana (thale cress)	AT3G04220	NP_187072.2
Arabidopsis thaliana (thale cress)	AT4G16940	NP_193427.4
Arabidopsis thaliana (thale cress)	AT4G16960	NP_193429.1
Arabidopsis thaliana (thale cress)	AT4G19470	NP_193682.1
Arabidopsis thaliana (thale cress)	MEKK1	NP_192590.1
Arabidopsis thaliana (thale cress)	DAR6	NP_201463.1
Arabidopsis thaliana (thale cress)	DAR7	NP_201462.2
Arabidopsis thaliana (thale cress)	TTR1	NP_851133.1
Arabidopsis thaliana (thale cress)	AT4G36150	NP_195338.1
Arabidopsis thaliana (thale cress)	AT1G27170	NP_001154372.1
Arabidopsis thaliana (thale cress)	RLM3	NP_849399.1
Arabidopsis thaliana (thale cress)	AT5G18360	NP_197337.1
Arabidopsis thaliana (thale cress)	AT1G65850	NP_001185325.1
Arabidopsis thaliana (thale cress)	AT5G22690	NP_197661.1
Arabidopsis thaliana (thale cress)	AT3G44630	NP_850655.1
Arabidopsis thaliana (thale cress)	AT1G17610	NP_173204.1
Arabidopsis thaliana (thale cress)	PP2-A7	NP_199322.1
Arabidopsis thaliana (thale cress)	AT2G03300	NP_178429.1
Arabidopsis thaliana (thale cress)	AT5G44910	NP_199304.1
Arabidopsis thaliana (thale cress)	AT5G45220	NP_199335.1
Arabidopsis thaliana (thale cress)	AT2G03030	NP_178403.1
Arabidopsis thaliana (thale cress)	AT5G44920	NP_851131.1
Arabidopsis thaliana (thale cress)	AT5G45240	NP_199337.2
Arabidopsis thaliana (thale cress)	AT5G48770	NP_199688.1
Arabidopsis thaliana (thale cress)	AT5G49140	NP_199725.1
Arabidopsis thaliana (thale cress)	AT1G63730	NP_176560.1
Arabidopsis thaliana (thale cress)	AT4G14370	NP_193173.4
Arabidopsis thaliana (thale cress)	AT1G57650	NP_001185254.1
Arabidopsis thaliana (thale cress)	AT3G25510	NP_189178.1
Arabidopsis thaliana (thale cress)	AT1G57670	NP_176080.1
Arabidopsis thaliana (thale cress)	AT2G32140	NP_180773.3
Arabidopsis thaliana (thale cress)	AT4G16900	NP_193424.2
Arabidopsis thaliana (thale cress)	AT1G72850	NP_177428.1
Arabidopsis thaliana (thale cress)	AT1G72890	NP_001185387.1
Arabidopsis thaliana (thale cress)	AT1G17615	NP_173205.1
Arabidopsis thaliana (thale cress)	AT2G17050	NP_179297.2
Arabidopsis thaliana (thale cress)	RPP1	NP_190034.2
Arabidopsis thaliana (thale cress)	RPP5	NP_193428.2
Arabidopsis thaliana (thale cress)	DAR5	NP_201464.2
Arabidopsis thaliana (thale cress)	AT1G27180	NP_174038.1
Arabidopsis thaliana (thale cress)	TAO1	NP_199264.1
Arabidopsis thaliana (thale cress)	AT5G11250	NP_196686.1
Arabidopsis thaliana (thale cress)	AT5G36930	NP_001119319.1
Arabidopsis thaliana (thale cress)	AT2G16870	NP_179279.1
Arabidopsis thaliana (thale cress)	AT5G40090	NP_198825.3
Arabidopsis thaliana (thale cress)	AT5G41750	NP_851117.2
Arabidopsis thaliana (thale cress)	AT5G45230	NP_199336.1
Arabidopsis thaliana (thale cress)	PP2-A4	NP_174654.1
Arabidopsis thaliana (thale cress)	PP2-A6	NP_199321.1
Arabidopsis thaliana (thale cress)	AT4G19920	NP_193725.3
Arabidopsis thaliana (thale cress)	AT3G51560	NP_190724.1
Arabidopsis thaliana (thale cress)	AT5G51630	NP_001190516.1
Arabidopsis thaliana (thale cress)	AT1G72840	NP_177427.2
Arabidopsis thaliana (thale cress)	AT1G63750	NP_001077769.1
Arabidopsis thaliana (thale cress)	AT1G63880	NP_176572.1
Arabidopsis thaliana (thale cress)	AT3G44400	NP_001118772.1
Arabidopsis thaliana (thale cress)	AT4G23510	NP_194082.2
Arabidopsis thaliana (thale cress)	AT4G19910	NP_974577.1
Arabidopsis thaliana (thale cress)	AT4G09420	NP_192680.1
Arabidopsis thaliana (thale cress)	AT1G72870	NP_177430.1
Arabidopsis thaliana (thale cress)	AT1G72900	NP_177433.1
Arabidopsis thaliana (thale cress)	AT3G44670	NP_001078237.1
Arabidopsis thaliana (thale cress)	AT4G09360	NP_192674.1
Arabidopsis thaliana (thale cress)	AT4G19500	NP_001190772.1
Arabidopsis thaliana (thale cress)	MEKK3	NP_192587.1
Arabidopsis thaliana (thale cress)	MAPKKK9	NP_192588.1
Arabidopsis thaliana (thale cress)	DAR3	NP_201465.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Although quantitative disease resistance (QDR) to Xanthomonas campestris is conferred by At5g22540 encoding for a protein of unknown function, QDR to XccCFBP6943 involves the well-known immune receptor pair RRS1/RPS4.
Title: Quantitative disease resistance to the bacterial pathogen Xanthomonas campestris involves an Arabidopsis immune receptor pair and a gene of unknown function.

The RRS1-leucine zipper motif is crucial for the regulation of the RPS4/RRS1 complex.
Title: Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1.

The Arabidopsis RRS1-R nucleotide-binding, leucine-rich repeat (NB-LRR) protein carries a C-terminal WRKY DNA binding domain and forms a receptor complex with RPS4, another NB-LRR protein. This complex detects the bacterial effectors AvrRps4 or PopP2 and then activates defense.
Title: A Plant Immune Receptor Detects Pathogen Effectors that Target WRKY Transcription Factors.

We show that many sushi(slh1) mutants carry mutations in RPS4, suggesting that RPS4 acts downstream or in a complex with RRS1. Interestingly, several mutations were identified in a domain C-terminal to the RPS4 LRR domain
Title: The nuclear immune receptor RPS4 is required for RRS1SLH1-dependent constitutive defense activation in Arabidopsis thaliana.

Here, we report that dual R proteins, RRS1 and RPS4, from A. thaliana ecotype Wassilewskija confer resistance to bacterial wilt in transgenic Brassica crops.
Title: Arabidopsis dual resistance proteins, both RPS4 and RRS1, are required for resistance to bacterial wilt in transgenic Brassica crops.

The following RRS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RRS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb20612	NM_123894.4 Latest version!	Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OAb20613	NM_001344604.1 Latest version!	Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OAb20613	NM_001085246.2 Latest version!	Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RRS1 ( NM_123894.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199339.2 Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.

Price & Availability↑

CloneID	OAb20612
Clone ID Related Accession (Same CDS sequence)	NM_123894.4
Accession Version	NM_123894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Disease resistance protein (TIR-NBS-LRR class)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123894.3.

ATGACCAATT GTGAAAAGGA TGAGGAATTC GTGTGCATCA GCTGCGTAGA AGAGGTACGG 
TACTCTTTCG TGAGCCACCT CTCTGAAGCT CTCCGTCGAA AAGGCATAAA TAATGTGGTC 
GTAGATGTAG ATATCGATGA TCTGCTTTTC AAGGAGTCTC AGGCAAAGAT CGAGAAAGCT 
GGGGTTTCTG TGATGGTTTT ACCCGGAAAC TGTGATCCTT CCGAGGTATG GCTTGACAAG 
TTCGCCAAGG TTCTCGAGTG CCAAAGGAAC AACAAGGACC AGGCGGTGGT TTCAGTGTTG 
TACGGTGACA GTCTATTACG GGACCAATGG CTTAGCGAGC TGGATTTCAG AGGCTTATCA 
CGAATTCACC AATCCAGGAA GGAATGTAGT GACTCTATAC TTGTAGAAGA GATTGTGAGA 
GATGTGTACG AGACGCACTT TTATGTTGGA CGAATTGGAA TCTATTCGAA GCTGCTGGAG 
ATTGAAAACA TGGTTAACAA GCAACCGATA GGCATCCGTT GTGTTGGAAT TTGGGGTATG 
CCTGGCATAG GAAAGACAAC ACTTGCTAAA GCAGTCTTTG ACCAAATGTC TAGCGCCTTT 
GATGCTTCTT GTTTTATCGA AGACTATGAC AAATCAATTC ATGAGAAGGG TCTTTATTGT 
TTGCTGGAGG AACAACTTTT GCCGGGCAAT GATGCAACCA TTATGAAACT GAGCTCGCTC 
AGAGACAGAT TGAACAGTAA GAGAGTTCTT GTTGTTCTCG ATGACGTGCG CAATGCTCTG 
GTTGGGGAGT CCTTTCTCGA GGGGTTTGAC TGGCTAGGAC CCGGAAGCCT GATCATCATA 
ACCTCTAGAG ATAAACAAGT GTTTTGCCTT TGCGGAATCA ATCAAATATA TGAGGTCCAG 
GGTTTAAATG AGAAAGAGGC TCGTCAACTT TTCTTGCTGT CTGCGTCTAT AAAGGAGGAT 
ATGGGAGAGC AGAATCTCCA GGAGTTGTCA GTGAGAGTAA TAAATTATGC TAATGGAAAC 
CCGTTAGCTA TCAGTGTTTA TGGAAGAGAG CTGAAAGGTA AGAAAAAACT CTCAGAAATG 
GAGACTGCAT TCCTCAAACT CAAGCGACGT CCTCCATTTA AGATTGTCGA TGCATTTAAA 
AGCACCTATG ACACACTCAG TGACAACGAA AAGAACATTT TTTTGGACAT AGCTTGTTTC 
TTCCAGGGAG AAAATGTCAA CTACGTGATA CAACTGCTTG AGGGTTGTGG TTTCTTTCCA 
CATGTTGAAA TTGATGTCCT TGTTGACAAG TGTCTGGTAA CTATTTCAGA AAACCGAGTT 
TGGTTGCATA AGCTGACCCA GGATATCGGC CGAGAAATCA TAAATGGAGA AACAGTACAG 
ATCGAGAGGC GCAGAAGACT GTGGGAACCT TGGAGCATCA AATATTTATT AGAATATAAT 
GAACACAAAG CAAATGGAGA ACCTAAAACA ACCTTCAAAC GTGCTCAGGG CTCTGAAGAG 
ATCGAAGGCC TGTTTCTAGA CACATCAAAC TTAAGATTTG ATCTGCAGCC CTCTGCCTTT 
AAGAATATGT TGAACCTTAG ATTGCTCAAA ATTTATTGTT CCAATCCTGA AGTCCATCCT 
GTAATCAATT TCCCAACAGG CTCTCTGCAT TCTCTTCCTA ATGAGCTAAG ACTCCTCCAT 
TGGGAGAACT ATCCTCTGAA ATCTTTGCCT CAGAATTTTG ATCCAAGGCA CCTTGTCGAA 
ATCAACATGC CGTATAGTCA ACTTCAGAAA CTTTGGGGTG GAACCAAGAA CCTGGAGATG 
TTGAGGACGA TCAGGCTTTG CCATTCCCAC CATCTAGTTG ATATCGATGA TCTCTTAAAA 
GCTGAAAATC TTGAGGTAAT TGATCTCCAA GGTTGTACGA GACTGCAGAA TTTCCCAGCC 
GCAGGTCGAT TGCTACGTCT ACGAGTTGTA AATCTCTCAG GTTGCATAAA GATTAAAAGT 
GTCCTAGAAA TTCCACCAAA TATTGAGAAA CTACATCTAC AGGGAACTGG CATATTAGCA 
TTACCAGTTT CCACTGTTAA GCCAAACCAT AGAGAGCTTG TGAATTTTCT AACAGAAATT 
CCGGGTCTTT CAGAGGAACT TGAGCGTTTA ACAAGTCTGC TGGAATCTAA CTCATCTTGT 
CAAGATCTTG GGAAGCTTAT TTGCTTGGAG CTGAAAGATT GCTCTTGTTT GCAGAGTCTG 
CCAAACATGG CTAATTTAGA TCTTAATGTT CTTGATCTCT CGGGTTGCTC AAGTCTTAAT 
TCTATTCAGG GTTTCCCTCG TTTTCTGAAA CAGTTATATC TTGGTGGCAC TGCAATAAGA 
GAAGTGCCAC AACTTCCTCA AAGTCTAGAA ATCTTGAATG CACATGGATC TTGTTTGCGA 
AGTCTGCCAA ACATGGCTAA TTTAGAATTT CTCAAAGTTC TTGATCTCTC TGGTTGCTCA 
GAGCTCGAGA CTATTCAGGG TTTTCCTCGG AACCTAAAAG AGTTATATTT TGCTGGCACT 
ACGTTAAGAG AAGTGCCCCA ACTTCCTTTA AGCCTAGAGG TCTTGAATGC ACATGGTTCT 
GACTCGGAGA AGCTTCCTAT GCATTACAAG TTCAACAATT TTTTCGATCT ATCTCAACAA 
GTGGTCAACG ATTTTTTATT GAAAACGCTG ACTTATGTAA AACACATACC AAGAGGGTAT 
ACGCAGGAAC TCATCAACAA AGCTCCGACT TTCAGCTTCA GTGCGCCCTC ACATACAAAT 
CAAAACGCCA CATTTGATCT GCAATCAGGA TCTTCTGTAA TGACACGACT AAATCATTCA 
TGGAGGAACA CGCTTGTGGG ATTTGGTATG CTGGTGGAAG TTGCATTTCC CGAGGACTAC 
TGTGATGCTA CAGATGTTGG CATAAGTTGT GTTTGCAGAT GGAGCAACAA AGAAGGCCGC 
TCTTGTAGGA TAGAAAGAAA ATTTCATTGT TGGGCACCAT GGCAAGTTGT TCCAAAAGTT 
CGAAAGGATC ATACGTTTGT CTTTAGTGAT GTCAACATGC GCCCAAGTAC CGGTGAAGGA 
AATGACCCTG ATATCTGGGC TGGATTAGTT GTATTTGAGT TCTTTCCTAT CAATCAGCAG 
ACAAAGTGTC TAAATGATAG GTTCACAGTG AGAAGATGTG GAGTCCGTGT AATAAATGTT 
GCAACTGGCA ATACAAGTCT TGAGAACATA GCACTAGTTT TGTCTTTGGA TCCAGTAGAG 
GTTTCCGGTT ATGAAGTATT GAGAGTCAGC TATGATGATT TACAGGAGAT GGATAAAGTT 
CTATTTCTTT ACATAGCGTC TTTGTTCAAT GACGAGGATG TTGATTTTGT GGCACCACTT 
ATTGCCGGTA TTGACTTGGA TGTTAGCTCT GGGCTCAAGG TCTTAGCCGA TGTGTCTCTC 
ATAAGTGTAT CATCAAATGG GGAAATAGTG ATGCATAGTT TGCAAAGACA AATGGGTAAA 
GAAATCCTCC ATGGACAATC CATGCTGCTG TCTGATTGTG AGAGTTCCAT GACCGAGAAT 
TTGTCTGACG TACCAAAAAA GAAGAAGAAA CATAGCGAAA GTAGGGTAAA GAAAGTGGTT 
TCCATACCGG CTATAGACGA GGGAGATCTA TGGACTTGGC GAAAGTACGG TCAAAAAGAC 
ATCTTAGGTT CTCGTTTTCC AAGGGGTTAC TACAGGTGCG CTTACAAGTT CACGCATGGT 
TGTAAAGCTA CAAAACAAGT CCAACGGAGC GAGACCGATT CAAACATGTT AGCTATTACT 
TACCTATCTG AGCATAACCA TCCACGGCCC ACTAAACGCA AGGCTCTCGC TGACTCCACT 
CGTTCCACTT CCTCCTCCAT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199339.2
CDS	75..3941
Translation	MTNCEKDEEFVCISCVEEVRYSFVSHLSEALRRKGINNVVVDVDIDDLLFKESQAKIEKAGVSVMVLPGNCDPSEVWLDKFAKVLECQRNNKDQAVVSVLYGDSLLRDQWLSELDFRGLSRIHQSRKECSDSILVEEIVRDVYETHFYVGRIGIYSKLLEIENMVNKQPIGIRCVGIWGMPGIGKTTLAKAVFDQMSSAFDASCFIEDYDKSIHEKGLYCLLEEQLLPGNDATIMKLSSLRDRLNSKRVLVVLDDVRNALVGESFLEGFDWLGPGSLIIITSRDKQVFCLCGINQIYEVQGLNEKEARQLFLLSASIKEDMGEQNLQELSVRVINYANGNPLAISVYGRELKGKKKLSEMETAFLKLKRRPPFKIVDAFKSTYDTLSDNEKNIFLDIACFFQGENVNYVIQLLEGCGFFPHVEIDVLVDKCLVTISENRVWLHKLTQDIGREIINGETVQIERRRRLWEPWSIKYLLEYNEHKANGEPKTTFKRAQGSEEIEGLFLDTSNLRFDLQPSAFKNMLNLRLLKIYCSNPEVHPVINFPTGSLHSLPNELRLLHWENYPLKSLPQNFDPRHLVEINMPYSQLQKLWGGTKNLEMLRTIRLCHSHHLVDIDDLLKAENLEVIDLQGCTRLQNFPAAGRLLRLRVVNLSGCIKIKSVLEIPPNIEKLHLQGTGILALPVSTVKPNHRELVNFLTEIPGLSEELERLTSLLESNSSCQDLGKLICLELKDCSCLQSLPNMANLDLNVLDLSGCSSLNSIQGFPRFLKQLYLGGTAIREVPQLPQSLEILNAHGSCLRSLPNMANLEFLKVLDLSGCSELETIQGFPRNLKELYFAGTTLREVPQLPLSLEVLNAHGSDSEKLPMHYKFNNFFDLSQQVVNDFLLKTLTYVKHIPRGYTQELINKAPTFSFSAPSHTNQNATFDLQSGSSVMTRLNHSWRNTLVGFGMLVEVAFPEDYCDATDVGISCVCRWSNKEGRSCRIERKFHCWAPWQVVPKVRKDHTFVFSDVNMRPSTGEGNDPDIWAGLVVFEFFPINQQTKCLNDRFTVRRCGVRVINVATGNTSLENIALVLSLDPVEVSGYEVLRVSYDDLQEMDKVLFLYIASLFNDEDVDFVAPLIAGIDLDVSSGLKVLADVSLISVSSNGEIVMHSLQRQMGKEILHGQSMLLSDCESSMTENLSDVPKKKKKHSESRVKKVVSIPAIDEGDLWTWRKYGQKDILGSRFPRGYYRCAYKFTHGCKATKQVQRSETDSNMLAITYLSEHNHPRPTKRKALADSTRSTSSSIC

Target ORF information:

RefSeq Version	NM_123894.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123894.4

ORF Insert Sequence:

ATGACCAATT GTGAAAAGGA TGAGGAATTC GTGTGCATCA GCTGCGTAGA AGAGGTACGG 
TACTCTTTCG TGAGCCACCT CTCTGAAGCT CTCCGTCGAA AAGGCATAAA TAATGTGGTC 
GTAGATGTAG ATATCGATGA TCTGCTTTTC AAGGAGTCTC AGGCAAAGAT CGAGAAAGCT 
GGGGTTTCTG TGATGGTTTT ACCCGGAAAC TGTGATCCTT CCGAGGTATG GCTTGACAAG 
TTCGCCAAGG TTCTCGAGTG CCAAAGGAAC AACAAGGACC AGGCGGTGGT TTCAGTGTTG 
TACGGTGACA GTCTATTACG GGACCAATGG CTTAGCGAGC TGGATTTCAG AGGCTTATCA 
CGAATTCACC AATCCAGGAA GGAATGTAGT GACTCTATAC TTGTAGAAGA GATTGTGAGA 
GATGTGTACG AGACGCACTT TTATGTTGGA CGAATTGGAA TCTATTCGAA GCTGCTGGAG 
ATTGAAAACA TGGTTAACAA GCAACCGATA GGCATCCGTT GTGTTGGAAT TTGGGGTATG 
CCTGGCATAG GAAAGACAAC ACTTGCTAAA GCAGTCTTTG ACCAAATGTC TAGCGCCTTT 
GATGCTTCTT GTTTTATCGA AGACTATGAC AAATCAATTC ATGAGAAGGG TCTTTATTGT 
TTGCTGGAGG AACAACTTTT GCCGGGCAAT GATGCAACCA TTATGAAACT GAGCTCGCTC 
AGAGACAGAT TGAACAGTAA GAGAGTTCTT GTTGTTCTCG ATGACGTGCG CAATGCTCTG 
GTTGGGGAGT CCTTTCTCGA GGGGTTTGAC TGGCTAGGAC CCGGAAGCCT GATCATCATA 
ACCTCTAGAG ATAAACAAGT GTTTTGCCTT TGCGGAATCA ATCAAATATA TGAGGTCCAG 
GGTTTAAATG AGAAAGAGGC TCGTCAACTT TTCTTGCTGT CTGCGTCTAT AAAGGAGGAT 
ATGGGAGAGC AGAATCTCCA GGAGTTGTCA GTGAGAGTAA TAAATTATGC TAATGGAAAC 
CCGTTAGCTA TCAGTGTTTA TGGAAGAGAG CTGAAAGGTA AGAAAAAACT CTCAGAAATG 
GAGACTGCAT TCCTCAAACT CAAGCGACGT CCTCCATTTA AGATTGTCGA TGCATTTAAA 
AGCACCTATG ACACACTCAG TGACAACGAA AAGAACATTT TTTTGGACAT AGCTTGTTTC 
TTCCAGGGAG AAAATGTCAA CTACGTGATA CAACTGCTTG AGGGTTGTGG TTTCTTTCCA 
CATGTTGAAA TTGATGTCCT TGTTGACAAG TGTCTGGTAA CTATTTCAGA AAACCGAGTT 
TGGTTGCATA AGCTGACCCA GGATATCGGC CGAGAAATCA TAAATGGAGA AACAGTACAG 
ATCGAGAGGC GCAGAAGACT GTGGGAACCT TGGAGCATCA AATATTTATT AGAATATAAT 
GAACACAAAG CAAATGGAGA ACCTAAAACA ACCTTCAAAC GTGCTCAGGG CTCTGAAGAG 
ATCGAAGGCC TGTTTCTAGA CACATCAAAC TTAAGATTTG ATCTGCAGCC CTCTGCCTTT 
AAGAATATGT TGAACCTTAG ATTGCTCAAA ATTTATTGTT CCAATCCTGA AGTCCATCCT 
GTAATCAATT TCCCAACAGG CTCTCTGCAT TCTCTTCCTA ATGAGCTAAG ACTCCTCCAT 
TGGGAGAACT ATCCTCTGAA ATCTTTGCCT CAGAATTTTG ATCCAAGGCA CCTTGTCGAA 
ATCAACATGC CGTATAGTCA ACTTCAGAAA CTTTGGGGTG GAACCAAGAA CCTGGAGATG 
TTGAGGACGA TCAGGCTTTG CCATTCCCAC CATCTAGTTG ATATCGATGA TCTCTTAAAA 
GCTGAAAATC TTGAGGTAAT TGATCTCCAA GGTTGTACGA GACTGCAGAA TTTCCCAGCC 
GCAGGTCGAT TGCTACGTCT ACGAGTTGTA AATCTCTCAG GTTGCATAAA GATTAAAAGT 
GTCCTAGAAA TTCCACCAAA TATTGAGAAA CTACATCTAC AGGGAACTGG CATATTAGCA 
TTACCAGTTT CCACTGTTAA GCCAAACCAT AGAGAGCTTG TGAATTTTCT AACAGAAATT 
CCGGGTCTTT CAGAGGAACT TGAGCGTTTA ACAAGTCTGC TGGAATCTAA CTCATCTTGT 
CAAGATCTTG GGAAGCTTAT TTGCTTGGAG CTGAAAGATT GCTCTTGTTT GCAGAGTCTG 
CCAAACATGG CTAATTTAGA TCTTAATGTT CTTGATCTCT CGGGTTGCTC AAGTCTTAAT 
TCTATTCAGG GTTTCCCTCG TTTTCTGAAA CAGTTATATC TTGGTGGCAC TGCAATAAGA 
GAAGTGCCAC AACTTCCTCA AAGTCTAGAA ATCTTGAATG CACATGGATC TTGTTTGCGA 
AGTCTGCCAA ACATGGCTAA TTTAGAATTT CTCAAAGTTC TTGATCTCTC TGGTTGCTCA 
GAGCTCGAGA CTATTCAGGG TTTTCCTCGG AACCTAAAAG AGTTATATTT TGCTGGCACT 
ACGTTAAGAG AAGTGCCCCA ACTTCCTTTA AGCCTAGAGG TCTTGAATGC ACATGGTTCT 
GACTCGGAGA AGCTTCCTAT GCATTACAAG TTCAACAATT TTTTCGATCT ATCTCAACAA 
GTGGTCAACG ATTTTTTATT GAAAACGCTG ACTTATGTAA AACACATACC AAGAGGGTAT 
ACGCAGGAAC TCATCAACAA AGCTCCGACT TTCAGCTTCA GTGCGCCCTC ACATACAAAT 
CAAAACGCCA CATTTGATCT GCAATCAGGA TCTTCTGTAA TGACACGACT AAATCATTCA 
TGGAGGAACA CGCTTGTGGG ATTTGGTATG CTGGTGGAAG TTGCATTTCC CGAGGACTAC 
TGTGATGCTA CAGATGTTGG CATAAGTTGT GTTTGCAGAT GGAGCAACAA AGAAGGCCGC 
TCTTGTAGGA TAGAAAGAAA ATTTCATTGT TGGGCACCAT GGCAAGTTGT TCCAAAAGTT 
CGAAAGGATC ATACGTTTGT CTTTAGTGAT GTCAACATGC GCCCAAGTAC CGGTGAAGGA 
AATGACCCTG ATATCTGGGC TGGATTAGTT GTATTTGAGT TCTTTCCTAT CAATCAGCAG 
ACAAAGTGTC TAAATGATAG GTTCACAGTG AGAAGATGTG GAGTCCGTGT AATAAATGTT 
GCAACTGGCA ATACAAGTCT TGAGAACATA GCACTAGTTT TGTCTTTGGA TCCAGTAGAG 
GTTTCCGGTT ATGAAGTATT GAGAGTCAGC TATGATGATT TACAGGAGAT GGATAAAGTT 
CTATTTCTTT ACATAGCGTC TTTGTTCAAT GACGAGGATG TTGATTTTGT GGCACCACTT 
ATTGCCGGTA TTGACTTGGA TGTTAGCTCT GGGCTCAAGG TCTTAGCCGA TGTGTCTCTC 
ATAAGTGTAT CATCAAATGG GGAAATAGTG ATGCATAGTT TGCAAAGACA AATGGGTAAA 
GAAATCCTCC ATGGACAATC CATGCTGCTG TCTGATTGTG AGAGTTCCAT GACCGAGAAT 
TTGTCTGACG TACCAAAAAA GAAGAAGAAA CATAGCGAAA GTAGGGTAAA GAAAGTGGTT 
TCCATACCGG CTATAGACGA GGGAGATCTA TGGACTTGGC GAAAGTACGG TCAAAAAGAC 
ATCTTAGGTT CTCGTTTTCC AAGGGGTTAC TACAGGTGCG CTTACAAGTT CACGCATGGT 
TGTAAAGCTA CAAAACAAGT CCAACGGAGC GAGACCGATT CAAACATGTT AGCTATTACT 
TACCTATCTG AGCATAACCA TCCACGGCCC ACTAAACGCA AGGCTCTCGC TGACTCCACT 
CGTTCCACTT CCTCCTCCAT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRS1 ( NM_001344604.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001318742.1 Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.

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CloneID	OAb20613
Clone ID Related Accession (Same CDS sequence)	NM_001344604.1 , NM_001085246.2
Accession Version	NM_001344604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Disease resistance protein (TIR-NBS-LRR class)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGACCAATT GTGAAAAGGA TGAGGAATTC GTGTGCATCA GCTGCGTAGA AGAGGTACGG 
TACTCTTTCG TGAGCCACCT CTCTGAAGCT CTCCGTCGAA AAGGCATAAA TAATGTGGTC 
GTAGATGTAG ATATCGATGA TCTGCTTTTC AAGGAGTCTC AGGCAAAGAT CGAGAAAGCT 
GGGGTTTCTG TGATGGTTTT ACCCGGAAAC TGTGATCCTT CCGAGGTATG GCTTGACAAG 
TTCGCCAAGG TTCTCGAGTG CCAAAGGAAC AACAAGGACC AGGCGGTGGT TTCAGTGTTG 
TACGGTGACA GTCTATTACG GGACCAATGG CTTAGCGAGC TGGATTTCAG AGGCTTATCA 
CGAATTCACC AATCCAGGAA GGAATGTAGT GACTCTATAC TTGTAGAAGA GATTGTGAGA 
GATGTGTACG AGACGCACTT TTATGTTGGA CGAATTGGAA TCTATTCGAA GCTGCTGGAG 
ATTGAAAACA TGGTTAACAA GCAACCGATA GGCATCCGTT GTGTTGGAAT TTGGGGTATG 
CCTGGCATAG GAAAGACAAC ACTTGCTAAA GCAGTCTTTG ACCAAATGTC TAGCGCCTTT 
GATGCTTCTT GTTTTATCGA AGACTATGAC AAATCAATTC ATGAGAAGGG TCTTTATTGT 
TTGCTGGAGG AACAACTTTT GCCGGGCAAT GATGCAACCA TTATGAAACT GAGCTCGCTC 
AGAGACAGAT TGAACAGTAA GAGAGTTCTT GTTGTTCTCG ATGACGTGCG CAATGCTCTG 
GTTGGGGAGT CCTTTCTCGA GGGGTTTGAC TGGCTAGGAC CCGGAAGCCT GATCATCATA 
ACCTCTAGAG ATAAACAAGT GTTTTGCCTT TGCGGAATCA ATCAAATATA TGAGGTCCAG 
GGTTTAAATG AGAAAGAGGC TCGTCAACTT TTCTTGCTGT CTGCGTCTAT AAAGGAGGAT 
ATGGGAGAGC AGAATCTCCA GGAGTTGTCA GTGAGAGTAA TAAATTATGC TAATGGAAAC 
CCGTTAGCTA TCAGTGTTTA TGGAAGAGAG CTGAAAGGTA AGAAAAAACT CTCAGAAATG 
GAGACTGCAT TCCTCAAACT CAAGCGACGT CCTCCATTTA AGATTGTCGA TGCATTTAAA 
AGCACCTATG ACACACTCAG TGACAACGAA AAGAACATTT TTTTGGACAT AGCTTGTTTC 
TTCCAGGGAG AAAATGTCAA CTACGTGATA CAACTGCTTG AGGGTTGTGG TTTCTTTCCA 
CATGTTGAAA TTGATGTCCT TGTTGACAAG TGTCTGGTAA CTATTTCAGA AAACCGAGTT 
TGGTTGCATA AGCTGACCCA GGATATCGGC CGAGAAATCA TAAATGGAGA AACAGTACAG 
ATCGAGAGGC GCAGAAGACT GTGGGAACCT TGGAGCATCA AATATTTATT AGAATATAAT 
GAACACAAAG CAAATGGAGA ACCTAAAACA ACCTTCAAAC GTGCTCAGGG CTCTGAAGAG 
ATCGAAGGCC TGTTTCTAGA CACATCAAAC TTAAGATTTG ATCTGCAGCC CTCTGCCTTT 
AAGAATATGT TGAACCTTAG ATTGCTCAAA ATTTATTGTT CCAATCCTGA AGTCCATCCT 
GTAATCAATT TCCCAACAGG CTCTCTGCAT TCTCTTCCTA ATGAGCTAAG ACTCCTCCAT 
TGGGAGAACT ATCCTCTGAA ATCTTTGCCT CAGAATTTTG ATCCAAGGCA CCTTGTCGAA 
ATCAACATGC CGTATAGTCA ACTTCAGAAA CTTTGGGGTG GAACCAAGAA CCTGGAGATG 
TTGAGGACGA TCAGGCTTTG CCATTCCCAC CATCTAGTTG ATATCGATGA TCTCTTAAAA 
GCTGAAAATC TTGAGGTAAT TGATCTCCAA GGTTGTACGA GACTGCAGAA TTTCCCAGCC 
GCAGGTCGAT TGCTACGTCT ACGAGTTGTA AATCTCTCAG GTTGCATAAA GATTAAAAGT 
GTCCTAGAAA TTCCACCAAA TATTGAGAAA CTACATCTAC AGGGAACTGG CATATTAGCA 
TTACCAGTTT CCACTGTTAA GCCAAACCAT AGAGAGCTTG TGAATTTTCT AACAGAAATT 
CCGGGTCTTT CAGAGGAACT TGAGCGTTTA ACAAGTCTGC TGGAATCTAA CTCATCTTGT 
CAAGATCTTG GGAAGCTTAT TTGCTTGGAG CTGAAAGATT GCTCTTGTTT GCAGAGTCTG 
CCAAACATGG CTAATTTAGA TCTTAATGTT CTTGATCTCT CGGGTTGCTC AAGTCTTAAT 
TCTATTCAGG GTTTCCCTCG TTTTCTGAAA CAGTTATATC TTGGTGGCAC TGCAATAAGA 
GAAGTGCCAC AACTTCCTCA AAGTCTAGAA ATCTTGAATG CACATGGATC TTGTTTGCGA 
AGTCTGCCAA ACATGGCTAA TTTAGAATTT CTCAAAGTTC TTGATCTCTC TGGTTGCTCA 
GAGCTCGAGA CTATTCAGGG TTTTCCTCGG AACCTAAAAG AGTTATATTT TGCTGGCACT 
ACGTTAAGAG AAGTGCCCCA ACTTCCTTTA AGCCTAGAGG TCTTGAATGC ACATGGTTCT 
GACTCGGAGA AGCTTCCTAT GCATTACAAG TTCAACAATT TTTTCGATCT ATCTCAACAA 
GTGGTCAACG ATTTTTTATT GAAAACGCTG ACTTATGTAA AACACATACC AAGAGGGTAT 
ACGCAGGAAC TCATCAACAA AGCTCCGACT TTCAGCTTCA GTGCGCCCTC ACATACAAAT 
CAAAACGCCA CATTTGATCT GCAATCAGGA TCTTCTGTAA TGACACGACT AAATCATTCA 
TGGAGGAACA CGCTTGTGGG ATTTGGTATG CTGGTGGAAG TTGCATTTCC CGAGGACTAC 
TGTGATGCTA CAGATGTTGG CATAAGTTGT GTTTGCAGAT GGAGCAACAA AGAAGGCCGC 
TCTTGTAGGA TAGAAAGAAA ATTTCATTGT TGGGCACCAT GGCAAGTTGT TCCAAAAGTT 
CGAAAGGATC ATACGTTTGT CTTTAGTGAT GTCAACATGC GCCCAAGTAC CGGTGAAGGA 
AATGACCCTG ATATCTGGGC TGGATTAGTT GTATTTGAGT TCTTTCCTAT CAATCAGCAG 
ACAAAGTGTC TAAATGATAG GTTCACAGTG AGAAGATGTG GAGTCCGTGT AATAAATGTT 
GCAACTGGCA ATACAAGTCT TGAGAACATA GCACTAGTTT TGTCTTTGGA TCCAGTAGAG 
GTTTCCGGTT ATGAAGTATT GAGAGTCAGC TATGATGATT TACAGGAGAT GGATAAAGTT 
CTATTTCTTT ACATAGCGTC TTTGTTCAAT GACGAGGATG TTGATTTTGT GGCACCACTT 
ATTGCCGGTA TTGACTTGGA TGTTAGCTCT GGGCTCAAGG TCTTAGCCGA TGTGTCTCTC 
ATAAGTGTAT CATCAAATGG GGAAATAGTG ATGCATAGTT TGCAAAGACA AATGGGTAAA 
GAAATCCTCC ATGGACAATC CATGCTGCTG TCTGATTGTG AGAGTTCCAT GACCGAGAAT 
TTGTCTGACG TACCAAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318742.1
CDS	75..3638
Translation	MTNCEKDEEFVCISCVEEVRYSFVSHLSEALRRKGINNVVVDVDIDDLLFKESQAKIEKAGVSVMVLPGNCDPSEVWLDKFAKVLECQRNNKDQAVVSVLYGDSLLRDQWLSELDFRGLSRIHQSRKECSDSILVEEIVRDVYETHFYVGRIGIYSKLLEIENMVNKQPIGIRCVGIWGMPGIGKTTLAKAVFDQMSSAFDASCFIEDYDKSIHEKGLYCLLEEQLLPGNDATIMKLSSLRDRLNSKRVLVVLDDVRNALVGESFLEGFDWLGPGSLIIITSRDKQVFCLCGINQIYEVQGLNEKEARQLFLLSASIKEDMGEQNLQELSVRVINYANGNPLAISVYGRELKGKKKLSEMETAFLKLKRRPPFKIVDAFKSTYDTLSDNEKNIFLDIACFFQGENVNYVIQLLEGCGFFPHVEIDVLVDKCLVTISENRVWLHKLTQDIGREIINGETVQIERRRRLWEPWSIKYLLEYNEHKANGEPKTTFKRAQGSEEIEGLFLDTSNLRFDLQPSAFKNMLNLRLLKIYCSNPEVHPVINFPTGSLHSLPNELRLLHWENYPLKSLPQNFDPRHLVEINMPYSQLQKLWGGTKNLEMLRTIRLCHSHHLVDIDDLLKAENLEVIDLQGCTRLQNFPAAGRLLRLRVVNLSGCIKIKSVLEIPPNIEKLHLQGTGILALPVSTVKPNHRELVNFLTEIPGLSEELERLTSLLESNSSCQDLGKLICLELKDCSCLQSLPNMANLDLNVLDLSGCSSLNSIQGFPRFLKQLYLGGTAIREVPQLPQSLEILNAHGSCLRSLPNMANLEFLKVLDLSGCSELETIQGFPRNLKELYFAGTTLREVPQLPLSLEVLNAHGSDSEKLPMHYKFNNFFDLSQQVVNDFLLKTLTYVKHIPRGYTQELINKAPTFSFSAPSHTNQNATFDLQSGSSVMTRLNHSWRNTLVGFGMLVEVAFPEDYCDATDVGISCVCRWSNKEGRSCRIERKFHCWAPWQVVPKVRKDHTFVFSDVNMRPSTGEGNDPDIWAGLVVFEFFPINQQTKCLNDRFTVRRCGVRVINVATGNTSLENIALVLSLDPVEVSGYEVLRVSYDDLQEMDKVLFLYIASLFNDEDVDFVAPLIAGIDLDVSSGLKVLADVSLISVSSNGEIVMHSLQRQMGKEILHGQSMLLSDCESSMTENLSDVPKK

Target ORF information:

RefSeq Version	NM_001344604.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001344604.1

ORF Insert Sequence:

ATGACCAATT GTGAAAAGGA TGAGGAATTC GTGTGCATCA GCTGCGTAGA AGAGGTACGG 
TACTCTTTCG TGAGCCACCT CTCTGAAGCT CTCCGTCGAA AAGGCATAAA TAATGTGGTC 
GTAGATGTAG ATATCGATGA TCTGCTTTTC AAGGAGTCTC AGGCAAAGAT CGAGAAAGCT 
GGGGTTTCTG TGATGGTTTT ACCCGGAAAC TGTGATCCTT CCGAGGTATG GCTTGACAAG 
TTCGCCAAGG TTCTCGAGTG CCAAAGGAAC AACAAGGACC AGGCGGTGGT TTCAGTGTTG 
TACGGTGACA GTCTATTACG GGACCAATGG CTTAGCGAGC TGGATTTCAG AGGCTTATCA 
CGAATTCACC AATCCAGGAA GGAATGTAGT GACTCTATAC TTGTAGAAGA GATTGTGAGA 
GATGTGTACG AGACGCACTT TTATGTTGGA CGAATTGGAA TCTATTCGAA GCTGCTGGAG 
ATTGAAAACA TGGTTAACAA GCAACCGATA GGCATCCGTT GTGTTGGAAT TTGGGGTATG 
CCTGGCATAG GAAAGACAAC ACTTGCTAAA GCAGTCTTTG ACCAAATGTC TAGCGCCTTT 
GATGCTTCTT GTTTTATCGA AGACTATGAC AAATCAATTC ATGAGAAGGG TCTTTATTGT 
TTGCTGGAGG AACAACTTTT GCCGGGCAAT GATGCAACCA TTATGAAACT GAGCTCGCTC 
AGAGACAGAT TGAACAGTAA GAGAGTTCTT GTTGTTCTCG ATGACGTGCG CAATGCTCTG 
GTTGGGGAGT CCTTTCTCGA GGGGTTTGAC TGGCTAGGAC CCGGAAGCCT GATCATCATA 
ACCTCTAGAG ATAAACAAGT GTTTTGCCTT TGCGGAATCA ATCAAATATA TGAGGTCCAG 
GGTTTAAATG AGAAAGAGGC TCGTCAACTT TTCTTGCTGT CTGCGTCTAT AAAGGAGGAT 
ATGGGAGAGC AGAATCTCCA GGAGTTGTCA GTGAGAGTAA TAAATTATGC TAATGGAAAC 
CCGTTAGCTA TCAGTGTTTA TGGAAGAGAG CTGAAAGGTA AGAAAAAACT CTCAGAAATG 
GAGACTGCAT TCCTCAAACT CAAGCGACGT CCTCCATTTA AGATTGTCGA TGCATTTAAA 
AGCACCTATG ACACACTCAG TGACAACGAA AAGAACATTT TTTTGGACAT AGCTTGTTTC 
TTCCAGGGAG AAAATGTCAA CTACGTGATA CAACTGCTTG AGGGTTGTGG TTTCTTTCCA 
CATGTTGAAA TTGATGTCCT TGTTGACAAG TGTCTGGTAA CTATTTCAGA AAACCGAGTT 
TGGTTGCATA AGCTGACCCA GGATATCGGC CGAGAAATCA TAAATGGAGA AACAGTACAG 
ATCGAGAGGC GCAGAAGACT GTGGGAACCT TGGAGCATCA AATATTTATT AGAATATAAT 
GAACACAAAG CAAATGGAGA ACCTAAAACA ACCTTCAAAC GTGCTCAGGG CTCTGAAGAG 
ATCGAAGGCC TGTTTCTAGA CACATCAAAC TTAAGATTTG ATCTGCAGCC CTCTGCCTTT 
AAGAATATGT TGAACCTTAG ATTGCTCAAA ATTTATTGTT CCAATCCTGA AGTCCATCCT 
GTAATCAATT TCCCAACAGG CTCTCTGCAT TCTCTTCCTA ATGAGCTAAG ACTCCTCCAT 
TGGGAGAACT ATCCTCTGAA ATCTTTGCCT CAGAATTTTG ATCCAAGGCA CCTTGTCGAA 
ATCAACATGC CGTATAGTCA ACTTCAGAAA CTTTGGGGTG GAACCAAGAA CCTGGAGATG 
TTGAGGACGA TCAGGCTTTG CCATTCCCAC CATCTAGTTG ATATCGATGA TCTCTTAAAA 
GCTGAAAATC TTGAGGTAAT TGATCTCCAA GGTTGTACGA GACTGCAGAA TTTCCCAGCC 
GCAGGTCGAT TGCTACGTCT ACGAGTTGTA AATCTCTCAG GTTGCATAAA GATTAAAAGT 
GTCCTAGAAA TTCCACCAAA TATTGAGAAA CTACATCTAC AGGGAACTGG CATATTAGCA 
TTACCAGTTT CCACTGTTAA GCCAAACCAT AGAGAGCTTG TGAATTTTCT AACAGAAATT 
CCGGGTCTTT CAGAGGAACT TGAGCGTTTA ACAAGTCTGC TGGAATCTAA CTCATCTTGT 
CAAGATCTTG GGAAGCTTAT TTGCTTGGAG CTGAAAGATT GCTCTTGTTT GCAGAGTCTG 
CCAAACATGG CTAATTTAGA TCTTAATGTT CTTGATCTCT CGGGTTGCTC AAGTCTTAAT 
TCTATTCAGG GTTTCCCTCG TTTTCTGAAA CAGTTATATC TTGGTGGCAC TGCAATAAGA 
GAAGTGCCAC AACTTCCTCA AAGTCTAGAA ATCTTGAATG CACATGGATC TTGTTTGCGA 
AGTCTGCCAA ACATGGCTAA TTTAGAATTT CTCAAAGTTC TTGATCTCTC TGGTTGCTCA 
GAGCTCGAGA CTATTCAGGG TTTTCCTCGG AACCTAAAAG AGTTATATTT TGCTGGCACT 
ACGTTAAGAG AAGTGCCCCA ACTTCCTTTA AGCCTAGAGG TCTTGAATGC ACATGGTTCT 
GACTCGGAGA AGCTTCCTAT GCATTACAAG TTCAACAATT TTTTCGATCT ATCTCAACAA 
GTGGTCAACG ATTTTTTATT GAAAACGCTG ACTTATGTAA AACACATACC AAGAGGGTAT 
ACGCAGGAAC TCATCAACAA AGCTCCGACT TTCAGCTTCA GTGCGCCCTC ACATACAAAT 
CAAAACGCCA CATTTGATCT GCAATCAGGA TCTTCTGTAA TGACACGACT AAATCATTCA 
TGGAGGAACA CGCTTGTGGG ATTTGGTATG CTGGTGGAAG TTGCATTTCC CGAGGACTAC 
TGTGATGCTA CAGATGTTGG CATAAGTTGT GTTTGCAGAT GGAGCAACAA AGAAGGCCGC 
TCTTGTAGGA TAGAAAGAAA ATTTCATTGT TGGGCACCAT GGCAAGTTGT TCCAAAAGTT 
CGAAAGGATC ATACGTTTGT CTTTAGTGAT GTCAACATGC GCCCAAGTAC CGGTGAAGGA 
AATGACCCTG ATATCTGGGC TGGATTAGTT GTATTTGAGT TCTTTCCTAT CAATCAGCAG 
ACAAAGTGTC TAAATGATAG GTTCACAGTG AGAAGATGTG GAGTCCGTGT AATAAATGTT 
GCAACTGGCA ATACAAGTCT TGAGAACATA GCACTAGTTT TGTCTTTGGA TCCAGTAGAG 
GTTTCCGGTT ATGAAGTATT GAGAGTCAGC TATGATGATT TACAGGAGAT GGATAAAGTT 
CTATTTCTTT ACATAGCGTC TTTGTTCAAT GACGAGGATG TTGATTTTGT GGCACCACTT 
ATTGCCGGTA TTGACTTGGA TGTTAGCTCT GGGCTCAAGG TCTTAGCCGA TGTGTCTCTC 
ATAAGTGTAT CATCAAATGG GGAAATAGTG ATGCATAGTT TGCAAAGACA AATGGGTAAA 
GAAATCCTCC ATGGACAATC CATGCTGCTG TCTGATTGTG AGAGTTCCAT GACCGAGAAT 
TTGTCTGACG TACCAAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRS1 ( NM_001085246.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001078715.1 Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.

Price & Availability↑

CloneID	OAb20613
Clone ID Related Accession (Same CDS sequence)	NM_001344604.1 , NM_001085246.2
Accession Version	NM_001085246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Disease resistance protein (TIR-NBS-LRR class)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001085246.1.

ATGACCAATT GTGAAAAGGA TGAGGAATTC GTGTGCATCA GCTGCGTAGA AGAGGTACGG 
TACTCTTTCG TGAGCCACCT CTCTGAAGCT CTCCGTCGAA AAGGCATAAA TAATGTGGTC 
GTAGATGTAG ATATCGATGA TCTGCTTTTC AAGGAGTCTC AGGCAAAGAT CGAGAAAGCT 
GGGGTTTCTG TGATGGTTTT ACCCGGAAAC TGTGATCCTT CCGAGGTATG GCTTGACAAG 
TTCGCCAAGG TTCTCGAGTG CCAAAGGAAC AACAAGGACC AGGCGGTGGT TTCAGTGTTG 
TACGGTGACA GTCTATTACG GGACCAATGG CTTAGCGAGC TGGATTTCAG AGGCTTATCA 
CGAATTCACC AATCCAGGAA GGAATGTAGT GACTCTATAC TTGTAGAAGA GATTGTGAGA 
GATGTGTACG AGACGCACTT TTATGTTGGA CGAATTGGAA TCTATTCGAA GCTGCTGGAG 
ATTGAAAACA TGGTTAACAA GCAACCGATA GGCATCCGTT GTGTTGGAAT TTGGGGTATG 
CCTGGCATAG GAAAGACAAC ACTTGCTAAA GCAGTCTTTG ACCAAATGTC TAGCGCCTTT 
GATGCTTCTT GTTTTATCGA AGACTATGAC AAATCAATTC ATGAGAAGGG TCTTTATTGT 
TTGCTGGAGG AACAACTTTT GCCGGGCAAT GATGCAACCA TTATGAAACT GAGCTCGCTC 
AGAGACAGAT TGAACAGTAA GAGAGTTCTT GTTGTTCTCG ATGACGTGCG CAATGCTCTG 
GTTGGGGAGT CCTTTCTCGA GGGGTTTGAC TGGCTAGGAC CCGGAAGCCT GATCATCATA 
ACCTCTAGAG ATAAACAAGT GTTTTGCCTT TGCGGAATCA ATCAAATATA TGAGGTCCAG 
GGTTTAAATG AGAAAGAGGC TCGTCAACTT TTCTTGCTGT CTGCGTCTAT AAAGGAGGAT 
ATGGGAGAGC AGAATCTCCA GGAGTTGTCA GTGAGAGTAA TAAATTATGC TAATGGAAAC 
CCGTTAGCTA TCAGTGTTTA TGGAAGAGAG CTGAAAGGTA AGAAAAAACT CTCAGAAATG 
GAGACTGCAT TCCTCAAACT CAAGCGACGT CCTCCATTTA AGATTGTCGA TGCATTTAAA 
AGCACCTATG ACACACTCAG TGACAACGAA AAGAACATTT TTTTGGACAT AGCTTGTTTC 
TTCCAGGGAG AAAATGTCAA CTACGTGATA CAACTGCTTG AGGGTTGTGG TTTCTTTCCA 
CATGTTGAAA TTGATGTCCT TGTTGACAAG TGTCTGGTAA CTATTTCAGA AAACCGAGTT 
TGGTTGCATA AGCTGACCCA GGATATCGGC CGAGAAATCA TAAATGGAGA AACAGTACAG 
ATCGAGAGGC GCAGAAGACT GTGGGAACCT TGGAGCATCA AATATTTATT AGAATATAAT 
GAACACAAAG CAAATGGAGA ACCTAAAACA ACCTTCAAAC GTGCTCAGGG CTCTGAAGAG 
ATCGAAGGCC TGTTTCTAGA CACATCAAAC TTAAGATTTG ATCTGCAGCC CTCTGCCTTT 
AAGAATATGT TGAACCTTAG ATTGCTCAAA ATTTATTGTT CCAATCCTGA AGTCCATCCT 
GTAATCAATT TCCCAACAGG CTCTCTGCAT TCTCTTCCTA ATGAGCTAAG ACTCCTCCAT 
TGGGAGAACT ATCCTCTGAA ATCTTTGCCT CAGAATTTTG ATCCAAGGCA CCTTGTCGAA 
ATCAACATGC CGTATAGTCA ACTTCAGAAA CTTTGGGGTG GAACCAAGAA CCTGGAGATG 
TTGAGGACGA TCAGGCTTTG CCATTCCCAC CATCTAGTTG ATATCGATGA TCTCTTAAAA 
GCTGAAAATC TTGAGGTAAT TGATCTCCAA GGTTGTACGA GACTGCAGAA TTTCCCAGCC 
GCAGGTCGAT TGCTACGTCT ACGAGTTGTA AATCTCTCAG GTTGCATAAA GATTAAAAGT 
GTCCTAGAAA TTCCACCAAA TATTGAGAAA CTACATCTAC AGGGAACTGG CATATTAGCA 
TTACCAGTTT CCACTGTTAA GCCAAACCAT AGAGAGCTTG TGAATTTTCT AACAGAAATT 
CCGGGTCTTT CAGAGGAACT TGAGCGTTTA ACAAGTCTGC TGGAATCTAA CTCATCTTGT 
CAAGATCTTG GGAAGCTTAT TTGCTTGGAG CTGAAAGATT GCTCTTGTTT GCAGAGTCTG 
CCAAACATGG CTAATTTAGA TCTTAATGTT CTTGATCTCT CGGGTTGCTC AAGTCTTAAT 
TCTATTCAGG GTTTCCCTCG TTTTCTGAAA CAGTTATATC TTGGTGGCAC TGCAATAAGA 
GAAGTGCCAC AACTTCCTCA AAGTCTAGAA ATCTTGAATG CACATGGATC TTGTTTGCGA 
AGTCTGCCAA ACATGGCTAA TTTAGAATTT CTCAAAGTTC TTGATCTCTC TGGTTGCTCA 
GAGCTCGAGA CTATTCAGGG TTTTCCTCGG AACCTAAAAG AGTTATATTT TGCTGGCACT 
ACGTTAAGAG AAGTGCCCCA ACTTCCTTTA AGCCTAGAGG TCTTGAATGC ACATGGTTCT 
GACTCGGAGA AGCTTCCTAT GCATTACAAG TTCAACAATT TTTTCGATCT ATCTCAACAA 
GTGGTCAACG ATTTTTTATT GAAAACGCTG ACTTATGTAA AACACATACC AAGAGGGTAT 
ACGCAGGAAC TCATCAACAA AGCTCCGACT TTCAGCTTCA GTGCGCCCTC ACATACAAAT 
CAAAACGCCA CATTTGATCT GCAATCAGGA TCTTCTGTAA TGACACGACT AAATCATTCA 
TGGAGGAACA CGCTTGTGGG ATTTGGTATG CTGGTGGAAG TTGCATTTCC CGAGGACTAC 
TGTGATGCTA CAGATGTTGG CATAAGTTGT GTTTGCAGAT GGAGCAACAA AGAAGGCCGC 
TCTTGTAGGA TAGAAAGAAA ATTTCATTGT TGGGCACCAT GGCAAGTTGT TCCAAAAGTT 
CGAAAGGATC ATACGTTTGT CTTTAGTGAT GTCAACATGC GCCCAAGTAC CGGTGAAGGA 
AATGACCCTG ATATCTGGGC TGGATTAGTT GTATTTGAGT TCTTTCCTAT CAATCAGCAG 
ACAAAGTGTC TAAATGATAG GTTCACAGTG AGAAGATGTG GAGTCCGTGT AATAAATGTT 
GCAACTGGCA ATACAAGTCT TGAGAACATA GCACTAGTTT TGTCTTTGGA TCCAGTAGAG 
GTTTCCGGTT ATGAAGTATT GAGAGTCAGC TATGATGATT TACAGGAGAT GGATAAAGTT 
CTATTTCTTT ACATAGCGTC TTTGTTCAAT GACGAGGATG TTGATTTTGT GGCACCACTT 
ATTGCCGGTA TTGACTTGGA TGTTAGCTCT GGGCTCAAGG TCTTAGCCGA TGTGTCTCTC 
ATAAGTGTAT CATCAAATGG GGAAATAGTG ATGCATAGTT TGCAAAGACA AATGGGTAAA 
GAAATCCTCC ATGGACAATC CATGCTGCTG TCTGATTGTG AGAGTTCCAT GACCGAGAAT 
TTGTCTGACG TACCAAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078715.1
CDS	75..3638
Translation	MTNCEKDEEFVCISCVEEVRYSFVSHLSEALRRKGINNVVVDVDIDDLLFKESQAKIEKAGVSVMVLPGNCDPSEVWLDKFAKVLECQRNNKDQAVVSVLYGDSLLRDQWLSELDFRGLSRIHQSRKECSDSILVEEIVRDVYETHFYVGRIGIYSKLLEIENMVNKQPIGIRCVGIWGMPGIGKTTLAKAVFDQMSSAFDASCFIEDYDKSIHEKGLYCLLEEQLLPGNDATIMKLSSLRDRLNSKRVLVVLDDVRNALVGESFLEGFDWLGPGSLIIITSRDKQVFCLCGINQIYEVQGLNEKEARQLFLLSASIKEDMGEQNLQELSVRVINYANGNPLAISVYGRELKGKKKLSEMETAFLKLKRRPPFKIVDAFKSTYDTLSDNEKNIFLDIACFFQGENVNYVIQLLEGCGFFPHVEIDVLVDKCLVTISENRVWLHKLTQDIGREIINGETVQIERRRRLWEPWSIKYLLEYNEHKANGEPKTTFKRAQGSEEIEGLFLDTSNLRFDLQPSAFKNMLNLRLLKIYCSNPEVHPVINFPTGSLHSLPNELRLLHWENYPLKSLPQNFDPRHLVEINMPYSQLQKLWGGTKNLEMLRTIRLCHSHHLVDIDDLLKAENLEVIDLQGCTRLQNFPAAGRLLRLRVVNLSGCIKIKSVLEIPPNIEKLHLQGTGILALPVSTVKPNHRELVNFLTEIPGLSEELERLTSLLESNSSCQDLGKLICLELKDCSCLQSLPNMANLDLNVLDLSGCSSLNSIQGFPRFLKQLYLGGTAIREVPQLPQSLEILNAHGSCLRSLPNMANLEFLKVLDLSGCSELETIQGFPRNLKELYFAGTTLREVPQLPLSLEVLNAHGSDSEKLPMHYKFNNFFDLSQQVVNDFLLKTLTYVKHIPRGYTQELINKAPTFSFSAPSHTNQNATFDLQSGSSVMTRLNHSWRNTLVGFGMLVEVAFPEDYCDATDVGISCVCRWSNKEGRSCRIERKFHCWAPWQVVPKVRKDHTFVFSDVNMRPSTGEGNDPDIWAGLVVFEFFPINQQTKCLNDRFTVRRCGVRVINVATGNTSLENIALVLSLDPVEVSGYEVLRVSYDDLQEMDKVLFLYIASLFNDEDVDFVAPLIAGIDLDVSSGLKVLADVSLISVSSNGEIVMHSLQRQMGKEILHGQSMLLSDCESSMTENLSDVPKK

Target ORF information:

RefSeq Version	NM_001085246.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Disease resistance protein (TIR-NBS-LRR class) (RRS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085246.2

ORF Insert Sequence:

ATGACCAATT GTGAAAAGGA TGAGGAATTC GTGTGCATCA GCTGCGTAGA AGAGGTACGG 
TACTCTTTCG TGAGCCACCT CTCTGAAGCT CTCCGTCGAA AAGGCATAAA TAATGTGGTC 
GTAGATGTAG ATATCGATGA TCTGCTTTTC AAGGAGTCTC AGGCAAAGAT CGAGAAAGCT 
GGGGTTTCTG TGATGGTTTT ACCCGGAAAC TGTGATCCTT CCGAGGTATG GCTTGACAAG 
TTCGCCAAGG TTCTCGAGTG CCAAAGGAAC AACAAGGACC AGGCGGTGGT TTCAGTGTTG 
TACGGTGACA GTCTATTACG GGACCAATGG CTTAGCGAGC TGGATTTCAG AGGCTTATCA 
CGAATTCACC AATCCAGGAA GGAATGTAGT GACTCTATAC TTGTAGAAGA GATTGTGAGA 
GATGTGTACG AGACGCACTT TTATGTTGGA CGAATTGGAA TCTATTCGAA GCTGCTGGAG 
ATTGAAAACA TGGTTAACAA GCAACCGATA GGCATCCGTT GTGTTGGAAT TTGGGGTATG 
CCTGGCATAG GAAAGACAAC ACTTGCTAAA GCAGTCTTTG ACCAAATGTC TAGCGCCTTT 
GATGCTTCTT GTTTTATCGA AGACTATGAC AAATCAATTC ATGAGAAGGG TCTTTATTGT 
TTGCTGGAGG AACAACTTTT GCCGGGCAAT GATGCAACCA TTATGAAACT GAGCTCGCTC 
AGAGACAGAT TGAACAGTAA GAGAGTTCTT GTTGTTCTCG ATGACGTGCG CAATGCTCTG 
GTTGGGGAGT CCTTTCTCGA GGGGTTTGAC TGGCTAGGAC CCGGAAGCCT GATCATCATA 
ACCTCTAGAG ATAAACAAGT GTTTTGCCTT TGCGGAATCA ATCAAATATA TGAGGTCCAG 
GGTTTAAATG AGAAAGAGGC TCGTCAACTT TTCTTGCTGT CTGCGTCTAT AAAGGAGGAT 
ATGGGAGAGC AGAATCTCCA GGAGTTGTCA GTGAGAGTAA TAAATTATGC TAATGGAAAC 
CCGTTAGCTA TCAGTGTTTA TGGAAGAGAG CTGAAAGGTA AGAAAAAACT CTCAGAAATG 
GAGACTGCAT TCCTCAAACT CAAGCGACGT CCTCCATTTA AGATTGTCGA TGCATTTAAA 
AGCACCTATG ACACACTCAG TGACAACGAA AAGAACATTT TTTTGGACAT AGCTTGTTTC 
TTCCAGGGAG AAAATGTCAA CTACGTGATA CAACTGCTTG AGGGTTGTGG TTTCTTTCCA 
CATGTTGAAA TTGATGTCCT TGTTGACAAG TGTCTGGTAA CTATTTCAGA AAACCGAGTT 
TGGTTGCATA AGCTGACCCA GGATATCGGC CGAGAAATCA TAAATGGAGA AACAGTACAG 
ATCGAGAGGC GCAGAAGACT GTGGGAACCT TGGAGCATCA AATATTTATT AGAATATAAT 
GAACACAAAG CAAATGGAGA ACCTAAAACA ACCTTCAAAC GTGCTCAGGG CTCTGAAGAG 
ATCGAAGGCC TGTTTCTAGA CACATCAAAC TTAAGATTTG ATCTGCAGCC CTCTGCCTTT 
AAGAATATGT TGAACCTTAG ATTGCTCAAA ATTTATTGTT CCAATCCTGA AGTCCATCCT 
GTAATCAATT TCCCAACAGG CTCTCTGCAT TCTCTTCCTA ATGAGCTAAG ACTCCTCCAT 
TGGGAGAACT ATCCTCTGAA ATCTTTGCCT CAGAATTTTG ATCCAAGGCA CCTTGTCGAA 
ATCAACATGC CGTATAGTCA ACTTCAGAAA CTTTGGGGTG GAACCAAGAA CCTGGAGATG 
TTGAGGACGA TCAGGCTTTG CCATTCCCAC CATCTAGTTG ATATCGATGA TCTCTTAAAA 
GCTGAAAATC TTGAGGTAAT TGATCTCCAA GGTTGTACGA GACTGCAGAA TTTCCCAGCC 
GCAGGTCGAT TGCTACGTCT ACGAGTTGTA AATCTCTCAG GTTGCATAAA GATTAAAAGT 
GTCCTAGAAA TTCCACCAAA TATTGAGAAA CTACATCTAC AGGGAACTGG CATATTAGCA 
TTACCAGTTT CCACTGTTAA GCCAAACCAT AGAGAGCTTG TGAATTTTCT AACAGAAATT 
CCGGGTCTTT CAGAGGAACT TGAGCGTTTA ACAAGTCTGC TGGAATCTAA CTCATCTTGT 
CAAGATCTTG GGAAGCTTAT TTGCTTGGAG CTGAAAGATT GCTCTTGTTT GCAGAGTCTG 
CCAAACATGG CTAATTTAGA TCTTAATGTT CTTGATCTCT CGGGTTGCTC AAGTCTTAAT 
TCTATTCAGG GTTTCCCTCG TTTTCTGAAA CAGTTATATC TTGGTGGCAC TGCAATAAGA 
GAAGTGCCAC AACTTCCTCA AAGTCTAGAA ATCTTGAATG CACATGGATC TTGTTTGCGA 
AGTCTGCCAA ACATGGCTAA TTTAGAATTT CTCAAAGTTC TTGATCTCTC TGGTTGCTCA 
GAGCTCGAGA CTATTCAGGG TTTTCCTCGG AACCTAAAAG AGTTATATTT TGCTGGCACT 
ACGTTAAGAG AAGTGCCCCA ACTTCCTTTA AGCCTAGAGG TCTTGAATGC ACATGGTTCT 
GACTCGGAGA AGCTTCCTAT GCATTACAAG TTCAACAATT TTTTCGATCT ATCTCAACAA 
GTGGTCAACG ATTTTTTATT GAAAACGCTG ACTTATGTAA AACACATACC AAGAGGGTAT 
ACGCAGGAAC TCATCAACAA AGCTCCGACT TTCAGCTTCA GTGCGCCCTC ACATACAAAT 
CAAAACGCCA CATTTGATCT GCAATCAGGA TCTTCTGTAA TGACACGACT AAATCATTCA 
TGGAGGAACA CGCTTGTGGG ATTTGGTATG CTGGTGGAAG TTGCATTTCC CGAGGACTAC 
TGTGATGCTA CAGATGTTGG CATAAGTTGT GTTTGCAGAT GGAGCAACAA AGAAGGCCGC 
TCTTGTAGGA TAGAAAGAAA ATTTCATTGT TGGGCACCAT GGCAAGTTGT TCCAAAAGTT 
CGAAAGGATC ATACGTTTGT CTTTAGTGAT GTCAACATGC GCCCAAGTAC CGGTGAAGGA 
AATGACCCTG ATATCTGGGC TGGATTAGTT GTATTTGAGT TCTTTCCTAT CAATCAGCAG 
ACAAAGTGTC TAAATGATAG GTTCACAGTG AGAAGATGTG GAGTCCGTGT AATAAATGTT 
GCAACTGGCA ATACAAGTCT TGAGAACATA GCACTAGTTT TGTCTTTGGA TCCAGTAGAG 
GTTTCCGGTT ATGAAGTATT GAGAGTCAGC TATGATGATT TACAGGAGAT GGATAAAGTT 
CTATTTCTTT ACATAGCGTC TTTGTTCAAT GACGAGGATG TTGATTTTGT GGCACCACTT 
ATTGCCGGTA TTGACTTGGA TGTTAGCTCT GGGCTCAAGG TCTTAGCCGA TGTGTCTCTC 
ATAAGTGTAT CATCAAATGG GGAAATAGTG ATGCATAGTT TGCAAAGACA AATGGGTAAA 
GAAATCCTCC ATGGACAATC CATGCTGCTG TCTGATTGTG AGAGTTCCAT GACCGAGAAT 
TTGTCTGACG TACCAAAAAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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RRS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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