PAF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PAF1
Entrez Gene ID	834290
Full Name	proteasome alpha subunit F1
Synonyms	20S PROTEASOME SUBUNIT PAF1,ARS5,ARSENIC TOLERANCE 5,ATPSM30,MJB21.17,MJB21_17,proteasome alpha subunit F1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_123644.5	NP_199093.1	proteasome alpha subunit F1

Pathways from BioSystems

Homologies

	PSMA1	XP_001089332.2
Rattus norvegicus (Norway rat)	Psma1	NP_058974.1
Caenorhabditis elegans (roundworm)	pas-6	NP_504472.1
Neurospora crassa	NCU06712	XP_960892.1
Mus musculus (house mouse)	Psma1	NP_036095.1
Danio rerio (zebrafish)	psma1	NP_001003427.1
Eremothecium gossypii	AGOS_AGL336W	NP_986331.1
Saccharomyces cerevisiae (baker's yeast)	PRE5	NP_014045.1
Kluyveromyces lactis	KLLA0A10153g	XP_451444.1
Arabidopsis thaliana (thale cress)	PAF2	NP_175158.1
Oryza sativa (rice)	Os02g0133800	NP_001045808.1
Pan troglodytes (chimpanzee)	PSMA1	XP_508298.3
Canis lupus familiaris (dog)	PSMA1	XP_005633752.1
Bos taurus (cattle)	PSMA1	NP_001030387.1
Drosophila melanogaster (fruit fly)	Prosalpha6T	NP_609623.1
Schizosaccharomyces pombe (fission yeast)	SPAC6G10.04c	NP_594101.1
Magnaporthe oryzae (rice blast fungus)	MGG_04053	XP_003719786.1
Homo sapiens (human)	PSMA1	NP_002777.1
Gallus gallus (chicken)	PSMA1	NP_990351.1
Arabidopsis thaliana (thale cress)	PAF1	NP_199093.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The SNW Domain of SKIP Is Required for Its Integration into the Spliceosome and Its Interaction with the Paf1 Complex in Arabidopsis
Title: The SNW Domain of SKIP Is Required for Its Integration into the Spliceosome and Its Interaction with the Paf1 Complex in Arabidopsis.

SKIP interacted with the Paf1c to modulate the expression of FLC clade genes at the transcriptional level.
Title: SKIP Interacts with the Paf1 Complex to Regulate Flowering via the Activation of FLC Transcription in Arabidopsis.

ARS5 encodes the proteasome alpha subunit F1 protein (PAF1) of the 26S proteasome complex, and is also a negative regulator of thiol-mediated arsenic tolerance.
Title: ARS5 is a component of the 26S proteasome complex, and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis.

The following PAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb20367	NM_123644.5 Latest version!	Arabidopsis thaliana proteasome alpha subunit F1 (PAF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAF1 ( NM_123644.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_199093.1 Arabidopsis thaliana proteasome alpha subunit F1 (PAF1), mRNA.

Price & Availability↑

CloneID	OAb20367
Clone ID Related Accession (Same CDS sequence)	NM_123644.5
Accession Version	NM_123644.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	proteasome alpha subunit F1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_123644.4.

ATGTTCAGAA ACCAATACGA CACAGATGTT ACGACATGGA GTCCAACAGG TCGTTTATTC 
CAAGTGGAAT ACGCCATGGA AGCAGTGAAG CAAGGATCTG CAGCAATTGG ACTCAGATCT 
CGTTCTCATG TCGTTCTCGC TTGTGTTAAC AAAGCTCAAT CGGAGCTTTC TTCGCATCAG 
AGGAAGATCT TTAAAGTCGA TGACCATATC GGTGTTGCAA TCGCTGGTCT CACAGCCGAC 
GGTCGTGTAT TGTCTCGGTA TATGAGATCG GAATCGATTA ATCACTCTTT TACTTATGAG 
TCTCCTCTTC CTGTTGGTCG TCTCGTTGTT CATCTTGCTG ATAAAGCTCA GGTCTGTACC 
CAACGATCAT GGAAACGACC CTATGGTGTT GGTTTGCTGG TAGGTGGATT GGATGAGTCT 
GGAGCCCACC TTTACTACAA CTGTCCCAGC GGAAACTACT TCGAGTACCA AGCTTTTGCT 
ATTGGATCCC GTTCCCAAGC AGCAAAGACC TATCTGGAAC GCAGGTTTGA GAGCTTTGGG 
GATTCTTCTA GAGAAGATCT GATCAAAGAT GCTATTTTGG CTGTTAGGGA GACATTGCAA 
GGGGAAACAC TGAAGAGTTC TCTCTGCACA GTCGCTATTC TAGGAGTCGA TGAACCGTTC 
CACTTCCTGG ACCAGGAAGC AATACAGAAA GTGATCGACA CATTTGAGAA AGTGCCAGAG 
GAAGAGGAAG GAGAAGGCGA GGCTGGTGAG GGTGAGGCCG AAGCTGCCGA GGCTGCACCA 
GCAGAACGAG GTGGTGGTGT TGCAGGTGAC CAAGACGTGG CTCCAATGGA AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_199093.1
CDS	182..1018
Translation	MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQRKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFGDSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEPFHFLDQEAIQKVIDTFEKVPEEEEGEGEAGEGEAEAAEAAPAERGGGVAGDQDVAPMEM

Target ORF information:

RefSeq Version	NM_123644.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana proteasome alpha subunit F1 (PAF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_123644.5

ORF Insert Sequence:

ATGTTCAGAA ACCAATACGA CACAGATGTT ACGACATGGA GTCCAACAGG TCGTTTATTC 
CAAGTGGAAT ACGCCATGGA AGCAGTGAAG CAAGGATCTG CAGCAATTGG ACTCAGATCT 
CGTTCTCATG TCGTTCTCGC TTGTGTTAAC AAAGCTCAAT CGGAGCTTTC TTCGCATCAG 
AGGAAGATCT TTAAAGTCGA TGACCATATC GGTGTTGCAA TCGCTGGTCT CACAGCCGAC 
GGTCGTGTAT TGTCTCGGTA TATGAGATCG GAATCGATTA ATCACTCTTT TACTTATGAG 
TCTCCTCTTC CTGTTGGTCG TCTCGTTGTT CATCTTGCTG ATAAAGCTCA GGTCTGTACC 
CAACGATCAT GGAAACGACC CTATGGTGTT GGTTTGCTGG TAGGTGGATT GGATGAGTCT 
GGAGCCCACC TTTACTACAA CTGTCCCAGC GGAAACTACT TCGAGTACCA AGCTTTTGCT 
ATTGGATCCC GTTCCCAAGC AGCAAAGACC TATCTGGAAC GCAGGTTTGA GAGCTTTGGG 
GATTCTTCTA GAGAAGATCT GATCAAAGAT GCTATTTTGG CTGTTAGGGA GACATTGCAA 
GGGGAAACAC TGAAGAGTTC TCTCTGCACA GTCGCTATTC TAGGAGTCGA TGAACCGTTC 
CACTTCCTGG ACCAGGAAGC AATACAGAAA GTGATCGACA CATTTGAGAA AGTGCCAGAG 
GAAGAGGAAG GAGAAGGCGA GGCTGGTGAG GGTGAGGCCG AAGCTGCCGA GGCTGCACCA 
GCAGAACGAG GTGGTGGTGT TGCAGGTGAC CAAGACGTGG CTCCAATGGA AATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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PAF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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