AT5G25230 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT5G25230
Entrez Gene ID	832594
Full Name	Ribosomal protein S5/Elongation factor G/III/V family protein
Synonyms	F21J6.106,F21J6_106
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_122432.2	NP_197905.1	Ribosomal protein S5/Elongation factor G/III/V family protein
NM_001343914.1	NP_001318643.1	Ribosomal protein S5/Elongation factor G/III/V family protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EFTUD2	XP_005624439.1
Saccharomyces cerevisiae (baker's yeast)	SNU114	NP_012748.1
Eremothecium gossypii	AGOS_AEL124W	NP_984737.1
Schizosaccharomyces pombe (fission yeast)	cwf10	NP_596689.2
Xenopus tropicalis (tropical clawed frog)	eftud2	NP_001025537.1
Homo sapiens (human)	EFTUD2	NP_001245282.1
Pan troglodytes (chimpanzee)	EFTUD2	XP_001145980.1
Oryza sativa (rice)	Os06g0608300	NP_001058038.1
Rattus norvegicus (Norway rat)	Eftud2	XP_213492.3
Arabidopsis thaliana (thale cress)	MEE5	NP_172112.1
Arabidopsis thaliana (thale cress)	AT5G25230	NP_197905.1
Mus musculus (house mouse)	Eftud2	NP_001103465.1
Danio rerio (zebrafish)	eftud2	NP_956802.2
Drosophila melanogaster (fruit fly)	CG4849	NP_651605.1
	EFTUD2	XP_001114964.1
Bos taurus (cattle)	EFTUD2	NP_001076865.1
Gallus gallus (chicken)	EFTUD2	NP_001026672.1
Caenorhabditis elegans (roundworm)	eftu-2	NP_498308.1
Kluyveromyces lactis	KLLA0A06688g	XP_451298.1
Magnaporthe oryzae (rice blast fungus)	MGG_13500	XP_003716278.1
Neurospora crassa	NCU02572	XP_965703.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT5G25230 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT5G25230 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb19495	NM_122432.2 Latest version!	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (AT5G25230), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAb19495	NM_001343914.1 Latest version!	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (AT5G25230), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT5G25230 ( NM_122432.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_197905.1 Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (AT5G25230), partial mRNA.

Price & Availability↑

CloneID	OAb19495
Clone ID Related Accession (Same CDS sequence)	NM_122432.2 , NM_001343914.1
Accession Version	NM_122432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Ribosomal protein S5/Elongation factor G/III/V family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_122432.1. COMPLETENESS: incomplete on the 5' end.

ATGGATGGTA GCTTGTATGG TGAGTGTGGT AACTATATTG GACCTGAGAT TGAATCTGAC 
AGAGATAGTG ATGATAGTGT AGAAGATGAA GATCTCCAAG AGCCTGGGGG TTCTAATGGC 
TGGATTACCA CTATCAATGA GAACCAAAAT ATTGTTCTTC CTGAGGATAA GAAGTACTAC 
CCTATTGCTA AGGAGGTTTA TGGTGAGGAT GTTGAGACCT TGGTTATGGA TGAAGATGAG 
CAGTCTCTTG AGCAACCCAT TATCAAACCC GTTAGAGATA TTAGATTTGA GGTGGGGGTC 
ATCAAGGATC AAACAACAAC GTATGTGTCA ACACTGTTTC TTATCGGCCT CATGTCTAAT 
CCTGCGCTTG TGAGGAATGT TGCTCTTGTG GGGCATCTCC AGCACGGCAA AACTGTCTTC 
ATGGATATGT TGGTAGAGCA GACGCATCGT ATGTCTACTT TTAATGCTGA AAACGACAAG 
CATATGAGAT ATACGGACAC AAGAGTCGAT GAGCAGGAGA GAAATATATC TATCAAGGCG 
GTTCCAATGT CTCTTGTCCT TGAGGACAGT AGATCCAAAT CATACCTGTG CAATATCATG 
GATACACCTG GAAATGTCAA TTTCTCTGAC GAAATGACTG CTTCTTTAAG ACTTGCTGAT 
GGTGCTGTTT TCATTGTTGA TGCTGCTCAA GGAGTCATGG TAAACACAGA GAGAGCTATA 
CGGCATGCAA TCCAGGATCA TCTCCCTATT GTTGTTGTGA TCAATAAGGT TGACAGGCTC 
ATAACTGAGC TCAAACTACC TCCAAGGGAT GCTTACTACA AGCTGAGGTA TACAATTGAA 
GTCATTAATA ACCATATATC TGCTGCTTCA ACAAATGCTG CAGACTTACC TCTTATAGAC 
CCTGCAGCTG GAAATGTATG TTTCGCCAGT GGTACAGCCG GATGGTCCTT CACATTACAA 
TCTTTTGCTA GAATGTATGC CAAGCTTCAT GGTGTGGCCA TGGACGTGGA TAAGTTTGCA 
TCTAGACTTT GGGGAGATGT ATATTATCAC CCTGATACTA GGGTATTCAA TACAAGTCCT 
CCAGTAGGTG GTGGAGAAAG AGCATTTGTT CAGTTTATTC TGGAGCCTCT ATACAAGATA 
TACAGCCAAG TGATAGGTGA ACATAAGAAG AGTGTAGAGA CCACCCTTGC AGAATTAGGA 
GTGACTCTGA GTAATAGTGC ATATAAGCTG AACGTCAGAC CTTTACTTAG GTTAGCCTGT 
AGTTCAGTGT TTGGTTCGGC ATCAGGGTTC ACTGATATGT TGGTAAAGCA CATTCCTTCA 
CCCAGAGAGG CTGCAGCTAG GAAAGTGGAC CATTCATACA CTGGAACCAA AGATTCTCCC 
ATTTATGAAT CAATGGTAGA ATGTGATCCA TCTGGACCTC TCATGGTTAA TGTGACAAAG 
TTGTACCCCA AATCAGATAC TAGTGTTTTT GATGTGTTTG GAAGAGTTTA TAGTGGTAGG 
CTTCAAACAG GGCAAAGCGT ACGTGTACTA GGAGAAGGGT ATTCACCTGA GGATGAGGAG 
GACATGACTA TAAAAGAAGT CACGAAGCTA TGGATATATC AAGCTAGGTA TAGAATACCA 
GTAAGCAGTG CCCCTCCCGG TTCTTGGGTT TTAATTGAAG GTGTCGATGC ATCCATCATG 
AAAACTGCAA CTCTGTGTAA TGCAAGTTAT GATGAAGATG TCTACATATT CCGGGCTCTC 
AAATTTAATA CCCTCCCAGT AGTCAAGACT GCAACTGAGC CTTTGAATCC TAGTGAGCTG 
CCTAAAATGG TGGAAGGGCT TAGGAAAATT AGCAAAAGCT ATCCCCTTGC GATAACCAAA 
GTTGAGGAGT CTGGGGAACA TACTATTCTA GGCACAGGTG AGTTGTACTT GGATTCCATC 
ATCAAGGACC TCAGGGAACT CTATTCAGAG GTCCAAGTAA AGGTTGCAGA TCCTGTGGTC 
TCCTTCTGTG AGACAGTGGT TGAATCTTCG TCAATGAAGT GCTTTGCTGA GACACCAAAC 
AAAAAGAACA AACTAACTAT GATTGCAGAG CCATTGGATA GAGGACTTGC AGAGGATATT 
GAGAATGGCG TTGTAAGCAT TGACTGGAAC AGAGTACAAC TTGGTGACTT CTTCAGGACG 
AAATATGACT GGGATTTACT TGCTGCACGT TCCATCTGGG CTTTTGGCCC TGACAAACAG 
GGGACAAACA TATTATTGGA TGACACCCTT CCAACTGAGG TGGACAGGAA CTTGATGATG 
GGAGTGAAAG ACTCCATTGT TCAAGGGTTC CAGTGGGGTG CTCGTGAAGG TCCCCTCTGT 
GATGAGCCCA TCAGAAATGT AAAGTTCAAG ATTGTTGATG CACGGATAGC ACCTGAGCCG 
TTGCATAGAG GATCTGGTCA GATGATTCCA ACGGCAAGAC GTGTTGCATA TTCAGCCTTT 
CTTATGGCAA CTCCAAGGCT AATGGAACCT GTCTACTATG TGGAGATACA AACACCAATA 
GACTGTGTAA CTGCCATCTA CACAGTTTTG TCACGTAGAC GTGGGTATGT CACATCTGAT 
GTTCCACAAC CCGGTACTCC TGCTTATATC GTGAAGGCTT TTCTGCCTGT GATAGAGTCG 
TTTGGGTTTG AGACAGATCT GAGGTACCAC ACTCAGGGCC AAGCCTTTTG CCTATCTGTG 
TTTGATCATT GGGCCATAGT CCCTGGAGAC CCTCTAGACA AAGCCATCCA ATTGCGCCCA 
CTTGAACCCG CCCCTATTCA GCATCTAGCT CGGGAATTCA TGGTCAAGAC CCGCCGTAGA 
AAGGGAATGA GCGAAGATGT CAGCGGGAAT AAATTCTTTG ATGAAGCGAT GATGGTAGAG 
CTTGCCCAGC AAACAGGCGA TCTCCATCTG CAGATGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_197905.1
CDS	1..2922
Translation	MDGSLYGECGNYIGPEIESDRDSDDSVEDEDLQEPGGSNGWITTINENQNIVLPEDKKYYPIAKEVYGEDVETLVMDEDEQSLEQPIIKPVRDIRFEVGVIKDQTTTYVSTLFLIGLMSNPALVRNVALVGHLQHGKTVFMDMLVEQTHRMSTFNAENDKHMRYTDTRVDEQERNISIKAVPMSLVLEDSRSKSYLCNIMDTPGNVNFSDEMTASLRLADGAVFIVDAAQGVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRYTIEVINNHISAASTNAADLPLIDPAAGNVCFASGTAGWSFTLQSFARMYAKLHGVAMDVDKFASRLWGDVYYHPDTRVFNTSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRLACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALKFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVVSFCETVVESSSMKCFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIWAFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSEDVSGNKFFDEAMMVELAQQTGDLHLQMI

Target ORF information:

RefSeq Version	NM_122432.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (AT5G25230), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_122432.2

ORF Insert Sequence:

ATGGATGGTA GCTTGTATGG TGAGTGTGGT AACTATATTG GACCTGAGAT TGAATCTGAC 
AGAGATAGTG ATGATAGTGT AGAAGATGAA GATCTCCAAG AGCCTGGGGG TTCTAATGGC 
TGGATTACCA CTATCAATGA GAACCAAAAT ATTGTTCTTC CTGAGGATAA GAAGTACTAC 
CCTATTGCTA AGGAGGTTTA TGGTGAGGAT GTTGAGACCT TGGTTATGGA TGAAGATGAG 
CAGTCTCTTG AGCAACCCAT TATCAAACCC GTTAGAGATA TTAGATTTGA GGTGGGGGTC 
ATCAAGGATC AAACAACAAC GTATGTGTCA ACACTGTTTC TTATCGGCCT CATGTCTAAT 
CCTGCGCTTG TGAGGAATGT TGCTCTTGTG GGGCATCTCC AGCACGGCAA AACTGTCTTC 
ATGGATATGT TGGTAGAGCA GACGCATCGT ATGTCTACTT TTAATGCTGA AAACGACAAG 
CATATGAGAT ATACGGACAC AAGAGTCGAT GAGCAGGAGA GAAATATATC TATCAAGGCG 
GTTCCAATGT CTCTTGTCCT TGAGGACAGT AGATCCAAAT CATACCTGTG CAATATCATG 
GATACACCTG GAAATGTCAA TTTCTCTGAC GAAATGACTG CTTCTTTAAG ACTTGCTGAT 
GGTGCTGTTT TCATTGTTGA TGCTGCTCAA GGAGTCATGG TAAACACAGA GAGAGCTATA 
CGGCATGCAA TCCAGGATCA TCTCCCTATT GTTGTTGTGA TCAATAAGGT TGACAGGCTC 
ATAACTGAGC TCAAACTACC TCCAAGGGAT GCTTACTACA AGCTGAGGTA TACAATTGAA 
GTCATTAATA ACCATATATC TGCTGCTTCA ACAAATGCTG CAGACTTACC TCTTATAGAC 
CCTGCAGCTG GAAATGTATG TTTCGCCAGT GGTACAGCCG GATGGTCCTT CACATTACAA 
TCTTTTGCTA GAATGTATGC CAAGCTTCAT GGTGTGGCCA TGGACGTGGA TAAGTTTGCA 
TCTAGACTTT GGGGAGATGT ATATTATCAC CCTGATACTA GGGTATTCAA TACAAGTCCT 
CCAGTAGGTG GTGGAGAAAG AGCATTTGTT CAGTTTATTC TGGAGCCTCT ATACAAGATA 
TACAGCCAAG TGATAGGTGA ACATAAGAAG AGTGTAGAGA CCACCCTTGC AGAATTAGGA 
GTGACTCTGA GTAATAGTGC ATATAAGCTG AACGTCAGAC CTTTACTTAG GTTAGCCTGT 
AGTTCAGTGT TTGGTTCGGC ATCAGGGTTC ACTGATATGT TGGTAAAGCA CATTCCTTCA 
CCCAGAGAGG CTGCAGCTAG GAAAGTGGAC CATTCATACA CTGGAACCAA AGATTCTCCC 
ATTTATGAAT CAATGGTAGA ATGTGATCCA TCTGGACCTC TCATGGTTAA TGTGACAAAG 
TTGTACCCCA AATCAGATAC TAGTGTTTTT GATGTGTTTG GAAGAGTTTA TAGTGGTAGG 
CTTCAAACAG GGCAAAGCGT ACGTGTACTA GGAGAAGGGT ATTCACCTGA GGATGAGGAG 
GACATGACTA TAAAAGAAGT CACGAAGCTA TGGATATATC AAGCTAGGTA TAGAATACCA 
GTAAGCAGTG CCCCTCCCGG TTCTTGGGTT TTAATTGAAG GTGTCGATGC ATCCATCATG 
AAAACTGCAA CTCTGTGTAA TGCAAGTTAT GATGAAGATG TCTACATATT CCGGGCTCTC 
AAATTTAATA CCCTCCCAGT AGTCAAGACT GCAACTGAGC CTTTGAATCC TAGTGAGCTG 
CCTAAAATGG TGGAAGGGCT TAGGAAAATT AGCAAAAGCT ATCCCCTTGC GATAACCAAA 
GTTGAGGAGT CTGGGGAACA TACTATTCTA GGCACAGGTG AGTTGTACTT GGATTCCATC 
ATCAAGGACC TCAGGGAACT CTATTCAGAG GTCCAAGTAA AGGTTGCAGA TCCTGTGGTC 
TCCTTCTGTG AGACAGTGGT TGAATCTTCG TCAATGAAGT GCTTTGCTGA GACACCAAAC 
AAAAAGAACA AACTAACTAT GATTGCAGAG CCATTGGATA GAGGACTTGC AGAGGATATT 
GAGAATGGCG TTGTAAGCAT TGACTGGAAC AGAGTACAAC TTGGTGACTT CTTCAGGACG 
AAATATGACT GGGATTTACT TGCTGCACGT TCCATCTGGG CTTTTGGCCC TGACAAACAG 
GGGACAAACA TATTATTGGA TGACACCCTT CCAACTGAGG TGGACAGGAA CTTGATGATG 
GGAGTGAAAG ACTCCATTGT TCAAGGGTTC CAGTGGGGTG CTCGTGAAGG TCCCCTCTGT 
GATGAGCCCA TCAGAAATGT AAAGTTCAAG ATTGTTGATG CACGGATAGC ACCTGAGCCG 
TTGCATAGAG GATCTGGTCA GATGATTCCA ACGGCAAGAC GTGTTGCATA TTCAGCCTTT 
CTTATGGCAA CTCCAAGGCT AATGGAACCT GTCTACTATG TGGAGATACA AACACCAATA 
GACTGTGTAA CTGCCATCTA CACAGTTTTG TCACGTAGAC GTGGGTATGT CACATCTGAT 
GTTCCACAAC CCGGTACTCC TGCTTATATC GTGAAGGCTT TTCTGCCTGT GATAGAGTCG 
TTTGGGTTTG AGACAGATCT GAGGTACCAC ACTCAGGGCC AAGCCTTTTG CCTATCTGTG 
TTTGATCATT GGGCCATAGT CCCTGGAGAC CCTCTAGACA AAGCCATCCA ATTGCGCCCA 
CTTGAACCCG CCCCTATTCA GCATCTAGCT CGGGAATTCA TGGTCAAGAC CCGCCGTAGA 
AAGGGAATGA GCGAAGATGT CAGCGGGAAT AAATTCTTTG ATGAAGCGAT GATGGTAGAG 
CTTGCCCAGC AAACAGGCGA TCTCCATCTG CAGATGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT5G25230 ( NM_001343914.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001318643.1 Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (AT5G25230), mRNA.

Price & Availability↑

CloneID	OAb19495
Clone ID Related Accession (Same CDS sequence)	NM_122432.2 , NM_001343914.1
Accession Version	NM_001343914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Ribosomal protein S5/Elongation factor G/III/V family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGATGGTA GCTTGTATGG TGAGTGTGGT AACTATATTG GACCTGAGAT TGAATCTGAC 
AGAGATAGTG ATGATAGTGT AGAAGATGAA GATCTCCAAG AGCCTGGGGG TTCTAATGGC 
TGGATTACCA CTATCAATGA GAACCAAAAT ATTGTTCTTC CTGAGGATAA GAAGTACTAC 
CCTATTGCTA AGGAGGTTTA TGGTGAGGAT GTTGAGACCT TGGTTATGGA TGAAGATGAG 
CAGTCTCTTG AGCAACCCAT TATCAAACCC GTTAGAGATA TTAGATTTGA GGTGGGGGTC 
ATCAAGGATC AAACAACAAC GTATGTGTCA ACACTGTTTC TTATCGGCCT CATGTCTAAT 
CCTGCGCTTG TGAGGAATGT TGCTCTTGTG GGGCATCTCC AGCACGGCAA AACTGTCTTC 
ATGGATATGT TGGTAGAGCA GACGCATCGT ATGTCTACTT TTAATGCTGA AAACGACAAG 
CATATGAGAT ATACGGACAC AAGAGTCGAT GAGCAGGAGA GAAATATATC TATCAAGGCG 
GTTCCAATGT CTCTTGTCCT TGAGGACAGT AGATCCAAAT CATACCTGTG CAATATCATG 
GATACACCTG GAAATGTCAA TTTCTCTGAC GAAATGACTG CTTCTTTAAG ACTTGCTGAT 
GGTGCTGTTT TCATTGTTGA TGCTGCTCAA GGAGTCATGG TAAACACAGA GAGAGCTATA 
CGGCATGCAA TCCAGGATCA TCTCCCTATT GTTGTTGTGA TCAATAAGGT TGACAGGCTC 
ATAACTGAGC TCAAACTACC TCCAAGGGAT GCTTACTACA AGCTGAGGTA TACAATTGAA 
GTCATTAATA ACCATATATC TGCTGCTTCA ACAAATGCTG CAGACTTACC TCTTATAGAC 
CCTGCAGCTG GAAATGTATG TTTCGCCAGT GGTACAGCCG GATGGTCCTT CACATTACAA 
TCTTTTGCTA GAATGTATGC CAAGCTTCAT GGTGTGGCCA TGGACGTGGA TAAGTTTGCA 
TCTAGACTTT GGGGAGATGT ATATTATCAC CCTGATACTA GGGTATTCAA TACAAGTCCT 
CCAGTAGGTG GTGGAGAAAG AGCATTTGTT CAGTTTATTC TGGAGCCTCT ATACAAGATA 
TACAGCCAAG TGATAGGTGA ACATAAGAAG AGTGTAGAGA CCACCCTTGC AGAATTAGGA 
GTGACTCTGA GTAATAGTGC ATATAAGCTG AACGTCAGAC CTTTACTTAG GTTAGCCTGT 
AGTTCAGTGT TTGGTTCGGC ATCAGGGTTC ACTGATATGT TGGTAAAGCA CATTCCTTCA 
CCCAGAGAGG CTGCAGCTAG GAAAGTGGAC CATTCATACA CTGGAACCAA AGATTCTCCC 
ATTTATGAAT CAATGGTAGA ATGTGATCCA TCTGGACCTC TCATGGTTAA TGTGACAAAG 
TTGTACCCCA AATCAGATAC TAGTGTTTTT GATGTGTTTG GAAGAGTTTA TAGTGGTAGG 
CTTCAAACAG GGCAAAGCGT ACGTGTACTA GGAGAAGGGT ATTCACCTGA GGATGAGGAG 
GACATGACTA TAAAAGAAGT CACGAAGCTA TGGATATATC AAGCTAGGTA TAGAATACCA 
GTAAGCAGTG CCCCTCCCGG TTCTTGGGTT TTAATTGAAG GTGTCGATGC ATCCATCATG 
AAAACTGCAA CTCTGTGTAA TGCAAGTTAT GATGAAGATG TCTACATATT CCGGGCTCTC 
AAATTTAATA CCCTCCCAGT AGTCAAGACT GCAACTGAGC CTTTGAATCC TAGTGAGCTG 
CCTAAAATGG TGGAAGGGCT TAGGAAAATT AGCAAAAGCT ATCCCCTTGC GATAACCAAA 
GTTGAGGAGT CTGGGGAACA TACTATTCTA GGCACAGGTG AGTTGTACTT GGATTCCATC 
ATCAAGGACC TCAGGGAACT CTATTCAGAG GTCCAAGTAA AGGTTGCAGA TCCTGTGGTC 
TCCTTCTGTG AGACAGTGGT TGAATCTTCG TCAATGAAGT GCTTTGCTGA GACACCAAAC 
AAAAAGAACA AACTAACTAT GATTGCAGAG CCATTGGATA GAGGACTTGC AGAGGATATT 
GAGAATGGCG TTGTAAGCAT TGACTGGAAC AGAGTACAAC TTGGTGACTT CTTCAGGACG 
AAATATGACT GGGATTTACT TGCTGCACGT TCCATCTGGG CTTTTGGCCC TGACAAACAG 
GGGACAAACA TATTATTGGA TGACACCCTT CCAACTGAGG TGGACAGGAA CTTGATGATG 
GGAGTGAAAG ACTCCATTGT TCAAGGGTTC CAGTGGGGTG CTCGTGAAGG TCCCCTCTGT 
GATGAGCCCA TCAGAAATGT AAAGTTCAAG ATTGTTGATG CACGGATAGC ACCTGAGCCG 
TTGCATAGAG GATCTGGTCA GATGATTCCA ACGGCAAGAC GTGTTGCATA TTCAGCCTTT 
CTTATGGCAA CTCCAAGGCT AATGGAACCT GTCTACTATG TGGAGATACA AACACCAATA 
GACTGTGTAA CTGCCATCTA CACAGTTTTG TCACGTAGAC GTGGGTATGT CACATCTGAT 
GTTCCACAAC CCGGTACTCC TGCTTATATC GTGAAGGCTT TTCTGCCTGT GATAGAGTCG 
TTTGGGTTTG AGACAGATCT GAGGTACCAC ACTCAGGGCC AAGCCTTTTG CCTATCTGTG 
TTTGATCATT GGGCCATAGT CCCTGGAGAC CCTCTAGACA AAGCCATCCA ATTGCGCCCA 
CTTGAACCCG CCCCTATTCA GCATCTAGCT CGGGAATTCA TGGTCAAGAC CCGCCGTAGA 
AAGGGAATGA GCGAAGATGT CAGCGGGAAT AAATTCTTTG ATGAAGCGAT GATGGTAGAG 
CTTGCCCAGC AAACAGGCGA TCTCCATCTG CAGATGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318643.1
CDS	87..3008
Translation	MDGSLYGECGNYIGPEIESDRDSDDSVEDEDLQEPGGSNGWITTINENQNIVLPEDKKYYPIAKEVYGEDVETLVMDEDEQSLEQPIIKPVRDIRFEVGVIKDQTTTYVSTLFLIGLMSNPALVRNVALVGHLQHGKTVFMDMLVEQTHRMSTFNAENDKHMRYTDTRVDEQERNISIKAVPMSLVLEDSRSKSYLCNIMDTPGNVNFSDEMTASLRLADGAVFIVDAAQGVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRYTIEVINNHISAASTNAADLPLIDPAAGNVCFASGTAGWSFTLQSFARMYAKLHGVAMDVDKFASRLWGDVYYHPDTRVFNTSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRLACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALKFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVVSFCETVVESSSMKCFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIWAFGPDKQGTNILLDDTLPTEVDRNLMMGVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSEDVSGNKFFDEAMMVELAQQTGDLHLQMI

Target ORF information:

RefSeq Version	NM_001343914.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (AT5G25230), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343914.1

ORF Insert Sequence:

ATGGATGGTA GCTTGTATGG TGAGTGTGGT AACTATATTG GACCTGAGAT TGAATCTGAC 
AGAGATAGTG ATGATAGTGT AGAAGATGAA GATCTCCAAG AGCCTGGGGG TTCTAATGGC 
TGGATTACCA CTATCAATGA GAACCAAAAT ATTGTTCTTC CTGAGGATAA GAAGTACTAC 
CCTATTGCTA AGGAGGTTTA TGGTGAGGAT GTTGAGACCT TGGTTATGGA TGAAGATGAG 
CAGTCTCTTG AGCAACCCAT TATCAAACCC GTTAGAGATA TTAGATTTGA GGTGGGGGTC 
ATCAAGGATC AAACAACAAC GTATGTGTCA ACACTGTTTC TTATCGGCCT CATGTCTAAT 
CCTGCGCTTG TGAGGAATGT TGCTCTTGTG GGGCATCTCC AGCACGGCAA AACTGTCTTC 
ATGGATATGT TGGTAGAGCA GACGCATCGT ATGTCTACTT TTAATGCTGA AAACGACAAG 
CATATGAGAT ATACGGACAC AAGAGTCGAT GAGCAGGAGA GAAATATATC TATCAAGGCG 
GTTCCAATGT CTCTTGTCCT TGAGGACAGT AGATCCAAAT CATACCTGTG CAATATCATG 
GATACACCTG GAAATGTCAA TTTCTCTGAC GAAATGACTG CTTCTTTAAG ACTTGCTGAT 
GGTGCTGTTT TCATTGTTGA TGCTGCTCAA GGAGTCATGG TAAACACAGA GAGAGCTATA 
CGGCATGCAA TCCAGGATCA TCTCCCTATT GTTGTTGTGA TCAATAAGGT TGACAGGCTC 
ATAACTGAGC TCAAACTACC TCCAAGGGAT GCTTACTACA AGCTGAGGTA TACAATTGAA 
GTCATTAATA ACCATATATC TGCTGCTTCA ACAAATGCTG CAGACTTACC TCTTATAGAC 
CCTGCAGCTG GAAATGTATG TTTCGCCAGT GGTACAGCCG GATGGTCCTT CACATTACAA 
TCTTTTGCTA GAATGTATGC CAAGCTTCAT GGTGTGGCCA TGGACGTGGA TAAGTTTGCA 
TCTAGACTTT GGGGAGATGT ATATTATCAC CCTGATACTA GGGTATTCAA TACAAGTCCT 
CCAGTAGGTG GTGGAGAAAG AGCATTTGTT CAGTTTATTC TGGAGCCTCT ATACAAGATA 
TACAGCCAAG TGATAGGTGA ACATAAGAAG AGTGTAGAGA CCACCCTTGC AGAATTAGGA 
GTGACTCTGA GTAATAGTGC ATATAAGCTG AACGTCAGAC CTTTACTTAG GTTAGCCTGT 
AGTTCAGTGT TTGGTTCGGC ATCAGGGTTC ACTGATATGT TGGTAAAGCA CATTCCTTCA 
CCCAGAGAGG CTGCAGCTAG GAAAGTGGAC CATTCATACA CTGGAACCAA AGATTCTCCC 
ATTTATGAAT CAATGGTAGA ATGTGATCCA TCTGGACCTC TCATGGTTAA TGTGACAAAG 
TTGTACCCCA AATCAGATAC TAGTGTTTTT GATGTGTTTG GAAGAGTTTA TAGTGGTAGG 
CTTCAAACAG GGCAAAGCGT ACGTGTACTA GGAGAAGGGT ATTCACCTGA GGATGAGGAG 
GACATGACTA TAAAAGAAGT CACGAAGCTA TGGATATATC AAGCTAGGTA TAGAATACCA 
GTAAGCAGTG CCCCTCCCGG TTCTTGGGTT TTAATTGAAG GTGTCGATGC ATCCATCATG 
AAAACTGCAA CTCTGTGTAA TGCAAGTTAT GATGAAGATG TCTACATATT CCGGGCTCTC 
AAATTTAATA CCCTCCCAGT AGTCAAGACT GCAACTGAGC CTTTGAATCC TAGTGAGCTG 
CCTAAAATGG TGGAAGGGCT TAGGAAAATT AGCAAAAGCT ATCCCCTTGC GATAACCAAA 
GTTGAGGAGT CTGGGGAACA TACTATTCTA GGCACAGGTG AGTTGTACTT GGATTCCATC 
ATCAAGGACC TCAGGGAACT CTATTCAGAG GTCCAAGTAA AGGTTGCAGA TCCTGTGGTC 
TCCTTCTGTG AGACAGTGGT TGAATCTTCG TCAATGAAGT GCTTTGCTGA GACACCAAAC 
AAAAAGAACA AACTAACTAT GATTGCAGAG CCATTGGATA GAGGACTTGC AGAGGATATT 
GAGAATGGCG TTGTAAGCAT TGACTGGAAC AGAGTACAAC TTGGTGACTT CTTCAGGACG 
AAATATGACT GGGATTTACT TGCTGCACGT TCCATCTGGG CTTTTGGCCC TGACAAACAG 
GGGACAAACA TATTATTGGA TGACACCCTT CCAACTGAGG TGGACAGGAA CTTGATGATG 
GGAGTGAAAG ACTCCATTGT TCAAGGGTTC CAGTGGGGTG CTCGTGAAGG TCCCCTCTGT 
GATGAGCCCA TCAGAAATGT AAAGTTCAAG ATTGTTGATG CACGGATAGC ACCTGAGCCG 
TTGCATAGAG GATCTGGTCA GATGATTCCA ACGGCAAGAC GTGTTGCATA TTCAGCCTTT 
CTTATGGCAA CTCCAAGGCT AATGGAACCT GTCTACTATG TGGAGATACA AACACCAATA 
GACTGTGTAA CTGCCATCTA CACAGTTTTG TCACGTAGAC GTGGGTATGT CACATCTGAT 
GTTCCACAAC CCGGTACTCC TGCTTATATC GTGAAGGCTT TTCTGCCTGT GATAGAGTCG 
TTTGGGTTTG AGACAGATCT GAGGTACCAC ACTCAGGGCC AAGCCTTTTG CCTATCTGTG 
TTTGATCATT GGGCCATAGT CCCTGGAGAC CCTCTAGACA AAGCCATCCA ATTGCGCCCA 
CTTGAACCCG CCCCTATTCA GCATCTAGCT CGGGAATTCA TGGTCAAGAC CCGCCGTAGA 
AAGGGAATGA GCGAAGATGT CAGCGGGAAT AAATTCTTTG ATGAAGCGAT GATGGTAGAG 
CTTGCCCAGC AAACAGGCGA TCTCCATCTG CAGATGATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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AT5G25230 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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