IMS2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	IMS2
Entrez Gene ID	832366
Full Name	2-isopropylmalate synthase 2
Synonyms	2-isopropylmalate synthase 2,MAM-L,MAM3,METHYLTHIOALKYMALATE SYNTHASE-LIKE,MYJ24.1,MYJ24_1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_122208.4	NP_197693.1	2-isopropylmalate synthase 2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	LYS20	NP_010099.1
Arabidopsis thaliana (thale cress)	MAM1	NP_197692.1
Kluyveromyces lactis	KLLA0F05489g	XP_455329.1
Eremothecium gossypii	AGOS_ADR107W	NP_984203.2
Arabidopsis thaliana (thale cress)	IMS2	NP_197693.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We studied the expression patterns of MAM1 and MAM3 genes in different organs and developmental stages using promoter-GUS fusion lines and qRT-PCR.
Title: Expression pattern of the glucosinolate side chain biosynthetic genes MAM1 and MAM3 of Arabidopsis thaliana in different organs and developmental stages.

MAM3 (formerly known as MAM-L)catalyzes the formation of all glucosinolate chain lengths in vivo as well as in vitro.
Title: MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis.

The following IMS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IMS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb19289	NM_122208.4 Latest version!	Arabidopsis thaliana 2-isopropylmalate synthase 2 (IMS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IMS2 ( NM_122208.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_197693.1 Arabidopsis thaliana 2-isopropylmalate synthase 2 (IMS2), mRNA.

Price & Availability↑

CloneID	OAb19289
Clone ID Related Accession (Same CDS sequence)	NM_122208.4
Accession Version	NM_122208.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	2-isopropylmalate synthase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_122208.3.

ATGGCTTCGT TACTTCTCAC ATCGTCGAGT ATGATAACCA CTAGCTGTCG CTCCATGGTT 
CTCCGGTCAG GGTTACCCAT CGGATCTTCT TTTCCCTCTC TTCGCCTGAC CCGTCCATAC 
GACAAGGCGA CTCTGTTCGT CTCATGTTGC TCCGCTGAGT CCAAAAAGGT GGCAACTAGT 
GCTACTGATC TCAAACCTAT CATGGAACGG AGGCCGGAGT ACATTCCGAA CAAGCTTCCC 
CACAAGAACT ATGTGCGTGT ATTAGACACG ACGCTTCGTG ACGGTGAACA ATCTCCCGGT 
GCAGCACTTA CTCCACCGCA GAAGTTAGAG ATTGCCAGGC AGCTAGCTAA ACTCCGAGTA 
GACATCATGG AAGTTGGTTT TCCGGTGTCG TCTGAGGAAG AATTCGAAGC TATTAAAACC 
ATCGCCAAGA CCGTGGGGAA CGAGGTGGAT GAGGAAACCG GTTACGTTCC TGTGATATGC 
GGCATTGCAC GATGCAAAAA GAGAGACATC GAGGCAACTT GGGAGGCACT GAAGTATGCG 
AAGAGGCCGA GGGTAATGCT ATTCACATCT ACTAGTGAAA TTCACATGAA ATATAAGTTG 
AAAAAGACTA AAGAAGAAGT GATCGAGATG GCCGTGAACA GTGTTAAGTA CGCTAAAAGC 
TTGGGCTTCA AAGACATCCA ATTTGGGTGC GAAGATGGCG GCAGGACGGA GAAAGATTTT 
ATATGTAAGA TTCTAGGAGA ATCGATAAAA GCGGGTGCAA CCACGGTGGG GTTTGCGGAC 
ACGGTCGGGA TCAACATGCC GCAAGAATTC GGAGAACTCG TGGCCTATGT CATAGAAAAC 
ACTCCAGGGG CTGATGATAT TGTCTTCGCC ATTCATTGTC ATAACGACCT TGGTGTTGCT 
ACCGCCAACA CAATATCCGG TATATGTGCG GGAGCAAGAC AAGTCGAAGT GACGATCAAC 
GGAATTGGTG AAAGAAGTGG GAATGCGCCG CTTGAAGAGG TCGTGATGGC TTTGAAATGT 
CGAGGAGAAT CTCTGATGGA TGGTGTTTAC ACAAAAATAG ACTCACGCCA AATTATGGCT 
ACAAGCAAGA TGGTTCAAGA GCATACCGGC ATGTATGTTC AACCACATAA GCCAATAGTT 
GGAGACAACT GTTTTGTTCA TGAGAGCGGC ATTCACCAGG ATGGAATATT GAAAAATCGA 
AGTACATATG AGATCTTATC ACCAGAAGAT GTTGGGATCG TAAAATCTGA AAATTCTGGC 
ATTGTTCTTG GAAAGCTTAG CGGACGTCAT GCTGTAAAAG ACCGGCTTAA AGAGTTGGGA 
TATGAAATCA GTGATGAGAA ATTCAACGAC ATCTTCTCAC GATACAGAGA ATTAACGAAG 
GACAAAAAGA GAATCACAGA CGCTGATCTG AAGGCATTAG TGGTGAACGG TGCTGAAATC 
TCATCAGAAA AATTAAACAG TAAAGGAATT AACGACCTTA TGTCAAGCCC TCAGATTTCC 
GCTGTTGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_197693.1
CDS	86..1597
Translation	MASLLLTSSSMITTSCRSMVLRSGLPIGSSFPSLRLTRPYDKATLFVSCCSAESKKVATSATDLKPIMERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQFGCEDGGRTEKDFICKILGESIKAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDIVFAIHCHNDLGVATANTISGICAGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHTGMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYEISDEKFNDIFSRYRELTKDKKRITDADLKALVVNGAEISSEKLNSKGINDLMSSPQISAVV

Target ORF information:

RefSeq Version	NM_122208.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana 2-isopropylmalate synthase 2 (IMS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_122208.4

ORF Insert Sequence:

ATGGCTTCGT TACTTCTCAC ATCGTCGAGT ATGATAACCA CTAGCTGTCG CTCCATGGTT 
CTCCGGTCAG GGTTACCCAT CGGATCTTCT TTTCCCTCTC TTCGCCTGAC CCGTCCATAC 
GACAAGGCGA CTCTGTTCGT CTCATGTTGC TCCGCTGAGT CCAAAAAGGT GGCAACTAGT 
GCTACTGATC TCAAACCTAT CATGGAACGG AGGCCGGAGT ACATTCCGAA CAAGCTTCCC 
CACAAGAACT ATGTGCGTGT ATTAGACACG ACGCTTCGTG ACGGTGAACA ATCTCCCGGT 
GCAGCACTTA CTCCACCGCA GAAGTTAGAG ATTGCCAGGC AGCTAGCTAA ACTCCGAGTA 
GACATCATGG AAGTTGGTTT TCCGGTGTCG TCTGAGGAAG AATTCGAAGC TATTAAAACC 
ATCGCCAAGA CCGTGGGGAA CGAGGTGGAT GAGGAAACCG GTTACGTTCC TGTGATATGC 
GGCATTGCAC GATGCAAAAA GAGAGACATC GAGGCAACTT GGGAGGCACT GAAGTATGCG 
AAGAGGCCGA GGGTAATGCT ATTCACATCT ACTAGTGAAA TTCACATGAA ATATAAGTTG 
AAAAAGACTA AAGAAGAAGT GATCGAGATG GCCGTGAACA GTGTTAAGTA CGCTAAAAGC 
TTGGGCTTCA AAGACATCCA ATTTGGGTGC GAAGATGGCG GCAGGACGGA GAAAGATTTT 
ATATGTAAGA TTCTAGGAGA ATCGATAAAA GCGGGTGCAA CCACGGTGGG GTTTGCGGAC 
ACGGTCGGGA TCAACATGCC GCAAGAATTC GGAGAACTCG TGGCCTATGT CATAGAAAAC 
ACTCCAGGGG CTGATGATAT TGTCTTCGCC ATTCATTGTC ATAACGACCT TGGTGTTGCT 
ACCGCCAACA CAATATCCGG TATATGTGCG GGAGCAAGAC AAGTCGAAGT GACGATCAAC 
GGAATTGGTG AAAGAAGTGG GAATGCGCCG CTTGAAGAGG TCGTGATGGC TTTGAAATGT 
CGAGGAGAAT CTCTGATGGA TGGTGTTTAC ACAAAAATAG ACTCACGCCA AATTATGGCT 
ACAAGCAAGA TGGTTCAAGA GCATACCGGC ATGTATGTTC AACCACATAA GCCAATAGTT 
GGAGACAACT GTTTTGTTCA TGAGAGCGGC ATTCACCAGG ATGGAATATT GAAAAATCGA 
AGTACATATG AGATCTTATC ACCAGAAGAT GTTGGGATCG TAAAATCTGA AAATTCTGGC 
ATTGTTCTTG GAAAGCTTAG CGGACGTCAT GCTGTAAAAG ACCGGCTTAA AGAGTTGGGA 
TATGAAATCA GTGATGAGAA ATTCAACGAC ATCTTCTCAC GATACAGAGA ATTAACGAAG 
GACAAAAAGA GAATCACAGA CGCTGATCTG AAGGCATTAG TGGTGAACGG TGCTGAAATC 
TCATCAGAAA AATTAAACAG TAAAGGAATT AACGACCTTA TGTCAAGCCC TCAGATTTCC 
GCTGTTGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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IMS2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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