ATMS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATMS1
Entrez Gene ID	831660
Full Name	Cobalamin-independent synthase family protein
Synonyms	ATCIMS,ATMETS,COBALAMIN-INDEPENDENT METHIONINE SYNTHASE,MPI7.60,MPI7_60,methionine synthesis 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001085130.2	NP_001078599.1	Cobalamin-independent synthase family protein
NM_121798.4	NP_197294.1	Cobalamin-independent synthase family protein

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MET6	NP_011015.3
Kluyveromyces lactis	KLLA0E20065g	XP_454859.1
Oryza sativa (rice)	Os12g0624000	NP_001067314.1
Neurospora crassa	NCU06512	XP_957152.1
Eremothecium gossypii	AGOS_ABR212C	NP_983161.2
Magnaporthe oryzae (rice blast fungus)	MGG_06712	XP_003709423.1
Schizosaccharomyces pombe (fission yeast)	met26	NP_593352.1
Oryza sativa (rice)	Os12g0623900	NP_001067313.1
Arabidopsis thaliana (thale cress)	ATMS1	NP_001078599.1
Arabidopsis thaliana (thale cress)	MS2	NP_001118564.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

x-ray crystallographic structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate
Title: Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate.

The following ATMS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATMS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb18734	NM_121798.4 Latest version!	Arabidopsis thaliana Cobalamin-independent synthase family protein (ATMS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb18734	NM_001085130.2 Latest version!	Arabidopsis thaliana Cobalamin-independent synthase family protein (ATMS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATMS1 ( NM_001085130.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001078599.1 Arabidopsis thaliana Cobalamin-independent synthase family protein (ATMS1), mRNA.

Price & Availability↑

CloneID	OAb18734
Clone ID Related Accession (Same CDS sequence)	NM_001085130.2 , NM_121798.4
Accession Version	NM_001085130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Cobalamin-independent synthase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_001085130.1.

ATGGCTTCAC ACATTGTTGG ATACCCACGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TCTGGGATGG TAAGAGCACT GCTGAGGATC TTCAGAAGGT GTCTGCTGAT 
CTCAGGTCAT CCATCTGGAA ACAGATGTCT GCCGCTGGGA CTAAGTTCAT CCCTAGCAAC 
ACCTTTGCTC ACTACGACCA GGTTCTTGAC ACCACCGCCA TGCTCGGTGC TGTTCCACCT 
AGGTATGGAT ACACTGGTGG TGAGATCGGC CTTGATGTTT ACTTCTCCAT GGCTAGAGGA 
AATGCCTCTG TGCCTGCCAT GGAAATGACC AAGTGGTTCG ACACCAACTA CCATTACATC 
GTCCCTGAGT TGGGCCCTGA GGTTAACTTC TCTTACGCAT CCCACAAGGC GGTGAATGAG 
TACAAGGAGG CCAAGGCTCT TGGTGTTGAC ACCGTCCCTG TACTTGTTGG CCCAGTCTCT 
TACTTGCTGC TTTCCAAGGC TGCCAAGGGT GTTGACAAGT CATTCGAACT TCTTTCTCTT 
CTCCCTAAGA TTCTCCCGAT CTACAAGGAA GTGATTACCG AGCTTAAGGC TGCTGGTGCC 
ACCTGGATTC AGCTTGACGA GCCTGTCCTT GTTATGGATC TTGAGGGTCA GAAACTCCAG 
GCCTTTACTG GTGCCTATGC TGAACTTGAA TCAACTCTTT CTGGTTTGAA TGTTCTTGTC 
GAGACCTACT TCGCTGATAT CCCTGCTGAG GCATACAAGA CCCTAACCTC ATTGAAGGGT 
GTGACTGCCT TTGGATTTGA TTTGGTTCGT GGCACCAAGA CCCTTGATTT GGTCAAGGCA 
GGTTTCCCTG AGGGAAAGTA CCTCTTTGCT GGTGTTGTTG ATGGAAGGAA CATCTGGGCC 
AACGACTTTG CTGCGTCCCT AAGCACCTTG CAGGCACTTG AAGGCATTGT TGGTAAAGAC 
AAGCTTGTGG TCTCAACCTC CTGCTCTCTT CTCCACACCG CTGTTGATCT TATCAATGAG 
ACTAAGCTTG ATGATGAAAT CAAGTCATGG TTGGCGTTTG CTGCCCAGAA GGTCGTTGAA 
GTGAACGCTT TGGCCAAGGC TTTGGCTGGT CAGAAGGACG AGGCTCTTTT CTCTGCCAAT 
GCTGCGGCTT TGGCTTCAAG GAGATCTTCC CCAAGAGTCA CCAACGAGGG TGTCCAGAAG 
GCTGCTGCTG CTTTGAAGGG ATCTGACCAC CGTCGTGCAA CCAATGTTAG TGCTAGGCTA 
GATGCTCAGC AGAAGAAGCT CAATCTCCCA ATCCTACCAA CCACAACCAT TGGATCCTTC 
CCACAGACTG TAGAGCTCAG GAGAGTTCGT CGTGAGTACA AGGCCAAAAA GGTCTCAGAG 
GAGGACTACG TTAAAGCCAT CAAGGAAGAG ATCAAGAAAG TTGTTGACCT CCAAGAGGAA 
CTTGACATCG ATGTTCTTGT CCACGGAGAG CCAGAGAGAA ACGACATGGT TGAGTACTTT 
GGTGAGCAGT TGTCTGGTTT TGCCTTCACT GCAAACGGAT GGGTCCAATC TTATGGATCT 
CGCTGTGTGA AGCCACCAGT TATCTATGGT GATGTGAGCC GTCCCAAGGC AATGACCGTC 
TTCTGGTCCG CAATGGCTCA GAGCATGACC TCTCGCCCAA TGAAGGGTAT GCTTACTGGT 
CCCGTCACCA TTCTCAACTG GTCCTTTGTC AGGAACGACC AGCCCAGGCA CGAAACCTGT 
TACCAGATCG CTTTGGCCAT CAAGGACGAA GTCGAGGATC TTGAGAAAGG TGGAATCGGT 
GTCATTCAGA TTGATGAGGC TGCACTTAGA GAAGGACTAC CACTCAGGAA ATCCGAGCAT 
GCTTTCTACT TGGACTGGGC CGTCCACTCC TTCAGAATCA CCAACTGTGG AGTCCAAGAC 
AGCACCCAGA TCCACACTCA CATGTGCTAC TCCCACTTCA ATGACATCAT ACACTCCATC 
ATCGACATGG ATGCTGATGT CATCACCATT GAGAACTCCA GGTCTGATGA GAAGCTTCTT 
TCCGTGTTCC GTGAAGGAGT GAAGTACGGT GCTGGAATCG GTCCAGGAGT CTACGACATC 
CACTCTCCAA GAATACCATC TTCTGAGGAA ATCGCAGACA GGGTCAACAA GATGCTTGCT 
GTCCTAGAGC AGAACATCCT TTGGGTTAAC CCTGACTGTG GTCTCAAGAC CCGTAAGTAC 
ACCGAGGTCA AGCCTGCACT CAAGAACATG GTTGATGCGG CTAAGCTCAT CCGCTCCCAG 
CTCGCCAGTG CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078599.1
CDS	228..2525
Translation	MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLQKVSADLRSSIWKQMSAAGTKFIPSNTFAHYDQVLDTTAMLGAVPPRYGYTGGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYIVPELGPEVNFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKAAKGVDKSFELLSLLPKILPIYKEVITELKAAGATWIQLDEPVLVMDLEGQKLQAFTGAYAELESTLSGLNVLVETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTLDLVKAGFPEGKYLFAGVVDGRNIWANDFAASLSTLQALEGIVGKDKLVVSTSCSLLHTAVDLINETKLDDEIKSWLAFAAQKVVEVNALAKALAGQKDEALFSANAAALASRRSSPRVTNEGVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKVSEEDYVKAIKEEIKKVVDLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKAMTVFWSAMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLAVLEQNILWVNPDCGLKTRKYTEVKPALKNMVDAAKLIRSQLASAK

Target ORF information:

RefSeq Version	NM_001085130.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Cobalamin-independent synthase family protein (ATMS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085130.2

ORF Insert Sequence:

ATGGCTTCAC ACATTGTTGG ATACCCACGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TCTGGGATGG TAAGAGCACT GCTGAGGATC TTCAGAAGGT GTCTGCTGAT 
CTCAGGTCAT CCATCTGGAA ACAGATGTCT GCCGCTGGGA CTAAGTTCAT CCCTAGCAAC 
ACCTTTGCTC ACTACGACCA GGTTCTTGAC ACCACCGCCA TGCTCGGTGC TGTTCCACCT 
AGGTATGGAT ACACTGGTGG TGAGATCGGC CTTGATGTTT ACTTCTCCAT GGCTAGAGGA 
AATGCCTCTG TGCCTGCCAT GGAAATGACC AAGTGGTTCG ACACCAACTA CCATTACATC 
GTCCCTGAGT TGGGCCCTGA GGTTAACTTC TCTTACGCAT CCCACAAGGC GGTGAATGAG 
TACAAGGAGG CCAAGGCTCT TGGTGTTGAC ACCGTCCCTG TACTTGTTGG CCCAGTCTCT 
TACTTGCTGC TTTCCAAGGC TGCCAAGGGT GTTGACAAGT CATTCGAACT TCTTTCTCTT 
CTCCCTAAGA TTCTCCCGAT CTACAAGGAA GTGATTACCG AGCTTAAGGC TGCTGGTGCC 
ACCTGGATTC AGCTTGACGA GCCTGTCCTT GTTATGGATC TTGAGGGTCA GAAACTCCAG 
GCCTTTACTG GTGCCTATGC TGAACTTGAA TCAACTCTTT CTGGTTTGAA TGTTCTTGTC 
GAGACCTACT TCGCTGATAT CCCTGCTGAG GCATACAAGA CCCTAACCTC ATTGAAGGGT 
GTGACTGCCT TTGGATTTGA TTTGGTTCGT GGCACCAAGA CCCTTGATTT GGTCAAGGCA 
GGTTTCCCTG AGGGAAAGTA CCTCTTTGCT GGTGTTGTTG ATGGAAGGAA CATCTGGGCC 
AACGACTTTG CTGCGTCCCT AAGCACCTTG CAGGCACTTG AAGGCATTGT TGGTAAAGAC 
AAGCTTGTGG TCTCAACCTC CTGCTCTCTT CTCCACACCG CTGTTGATCT TATCAATGAG 
ACTAAGCTTG ATGATGAAAT CAAGTCATGG TTGGCGTTTG CTGCCCAGAA GGTCGTTGAA 
GTGAACGCTT TGGCCAAGGC TTTGGCTGGT CAGAAGGACG AGGCTCTTTT CTCTGCCAAT 
GCTGCGGCTT TGGCTTCAAG GAGATCTTCC CCAAGAGTCA CCAACGAGGG TGTCCAGAAG 
GCTGCTGCTG CTTTGAAGGG ATCTGACCAC CGTCGTGCAA CCAATGTTAG TGCTAGGCTA 
GATGCTCAGC AGAAGAAGCT CAATCTCCCA ATCCTACCAA CCACAACCAT TGGATCCTTC 
CCACAGACTG TAGAGCTCAG GAGAGTTCGT CGTGAGTACA AGGCCAAAAA GGTCTCAGAG 
GAGGACTACG TTAAAGCCAT CAAGGAAGAG ATCAAGAAAG TTGTTGACCT CCAAGAGGAA 
CTTGACATCG ATGTTCTTGT CCACGGAGAG CCAGAGAGAA ACGACATGGT TGAGTACTTT 
GGTGAGCAGT TGTCTGGTTT TGCCTTCACT GCAAACGGAT GGGTCCAATC TTATGGATCT 
CGCTGTGTGA AGCCACCAGT TATCTATGGT GATGTGAGCC GTCCCAAGGC AATGACCGTC 
TTCTGGTCCG CAATGGCTCA GAGCATGACC TCTCGCCCAA TGAAGGGTAT GCTTACTGGT 
CCCGTCACCA TTCTCAACTG GTCCTTTGTC AGGAACGACC AGCCCAGGCA CGAAACCTGT 
TACCAGATCG CTTTGGCCAT CAAGGACGAA GTCGAGGATC TTGAGAAAGG TGGAATCGGT 
GTCATTCAGA TTGATGAGGC TGCACTTAGA GAAGGACTAC CACTCAGGAA ATCCGAGCAT 
GCTTTCTACT TGGACTGGGC CGTCCACTCC TTCAGAATCA CCAACTGTGG AGTCCAAGAC 
AGCACCCAGA TCCACACTCA CATGTGCTAC TCCCACTTCA ATGACATCAT ACACTCCATC 
ATCGACATGG ATGCTGATGT CATCACCATT GAGAACTCCA GGTCTGATGA GAAGCTTCTT 
TCCGTGTTCC GTGAAGGAGT GAAGTACGGT GCTGGAATCG GTCCAGGAGT CTACGACATC 
CACTCTCCAA GAATACCATC TTCTGAGGAA ATCGCAGACA GGGTCAACAA GATGCTTGCT 
GTCCTAGAGC AGAACATCCT TTGGGTTAAC CCTGACTGTG GTCTCAAGAC CCGTAAGTAC 
ACCGAGGTCA AGCCTGCACT CAAGAACATG GTTGATGCGG CTAAGCTCAT CCGCTCCCAG 
CTCGCCAGTG CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATMS1 ( NM_121798.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_197294.1 Arabidopsis thaliana Cobalamin-independent synthase family protein (ATMS1), mRNA.

Price & Availability↑

CloneID	OAb18734
Clone ID Related Accession (Same CDS sequence)	NM_001085130.2 , NM_121798.4
Accession Version	NM_121798.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Cobalamin-independent synthase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121798.3.

ATGGCTTCAC ACATTGTTGG ATACCCACGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TCTGGGATGG TAAGAGCACT GCTGAGGATC TTCAGAAGGT GTCTGCTGAT 
CTCAGGTCAT CCATCTGGAA ACAGATGTCT GCCGCTGGGA CTAAGTTCAT CCCTAGCAAC 
ACCTTTGCTC ACTACGACCA GGTTCTTGAC ACCACCGCCA TGCTCGGTGC TGTTCCACCT 
AGGTATGGAT ACACTGGTGG TGAGATCGGC CTTGATGTTT ACTTCTCCAT GGCTAGAGGA 
AATGCCTCTG TGCCTGCCAT GGAAATGACC AAGTGGTTCG ACACCAACTA CCATTACATC 
GTCCCTGAGT TGGGCCCTGA GGTTAACTTC TCTTACGCAT CCCACAAGGC GGTGAATGAG 
TACAAGGAGG CCAAGGCTCT TGGTGTTGAC ACCGTCCCTG TACTTGTTGG CCCAGTCTCT 
TACTTGCTGC TTTCCAAGGC TGCCAAGGGT GTTGACAAGT CATTCGAACT TCTTTCTCTT 
CTCCCTAAGA TTCTCCCGAT CTACAAGGAA GTGATTACCG AGCTTAAGGC TGCTGGTGCC 
ACCTGGATTC AGCTTGACGA GCCTGTCCTT GTTATGGATC TTGAGGGTCA GAAACTCCAG 
GCCTTTACTG GTGCCTATGC TGAACTTGAA TCAACTCTTT CTGGTTTGAA TGTTCTTGTC 
GAGACCTACT TCGCTGATAT CCCTGCTGAG GCATACAAGA CCCTAACCTC ATTGAAGGGT 
GTGACTGCCT TTGGATTTGA TTTGGTTCGT GGCACCAAGA CCCTTGATTT GGTCAAGGCA 
GGTTTCCCTG AGGGAAAGTA CCTCTTTGCT GGTGTTGTTG ATGGAAGGAA CATCTGGGCC 
AACGACTTTG CTGCGTCCCT AAGCACCTTG CAGGCACTTG AAGGCATTGT TGGTAAAGAC 
AAGCTTGTGG TCTCAACCTC CTGCTCTCTT CTCCACACCG CTGTTGATCT TATCAATGAG 
ACTAAGCTTG ATGATGAAAT CAAGTCATGG TTGGCGTTTG CTGCCCAGAA GGTCGTTGAA 
GTGAACGCTT TGGCCAAGGC TTTGGCTGGT CAGAAGGACG AGGCTCTTTT CTCTGCCAAT 
GCTGCGGCTT TGGCTTCAAG GAGATCTTCC CCAAGAGTCA CCAACGAGGG TGTCCAGAAG 
GCTGCTGCTG CTTTGAAGGG ATCTGACCAC CGTCGTGCAA CCAATGTTAG TGCTAGGCTA 
GATGCTCAGC AGAAGAAGCT CAATCTCCCA ATCCTACCAA CCACAACCAT TGGATCCTTC 
CCACAGACTG TAGAGCTCAG GAGAGTTCGT CGTGAGTACA AGGCCAAAAA GGTCTCAGAG 
GAGGACTACG TTAAAGCCAT CAAGGAAGAG ATCAAGAAAG TTGTTGACCT CCAAGAGGAA 
CTTGACATCG ATGTTCTTGT CCACGGAGAG CCAGAGAGAA ACGACATGGT TGAGTACTTT 
GGTGAGCAGT TGTCTGGTTT TGCCTTCACT GCAAACGGAT GGGTCCAATC TTATGGATCT 
CGCTGTGTGA AGCCACCAGT TATCTATGGT GATGTGAGCC GTCCCAAGGC AATGACCGTC 
TTCTGGTCCG CAATGGCTCA GAGCATGACC TCTCGCCCAA TGAAGGGTAT GCTTACTGGT 
CCCGTCACCA TTCTCAACTG GTCCTTTGTC AGGAACGACC AGCCCAGGCA CGAAACCTGT 
TACCAGATCG CTTTGGCCAT CAAGGACGAA GTCGAGGATC TTGAGAAAGG TGGAATCGGT 
GTCATTCAGA TTGATGAGGC TGCACTTAGA GAAGGACTAC CACTCAGGAA ATCCGAGCAT 
GCTTTCTACT TGGACTGGGC CGTCCACTCC TTCAGAATCA CCAACTGTGG AGTCCAAGAC 
AGCACCCAGA TCCACACTCA CATGTGCTAC TCCCACTTCA ATGACATCAT ACACTCCATC 
ATCGACATGG ATGCTGATGT CATCACCATT GAGAACTCCA GGTCTGATGA GAAGCTTCTT 
TCCGTGTTCC GTGAAGGAGT GAAGTACGGT GCTGGAATCG GTCCAGGAGT CTACGACATC 
CACTCTCCAA GAATACCATC TTCTGAGGAA ATCGCAGACA GGGTCAACAA GATGCTTGCT 
GTCCTAGAGC AGAACATCCT TTGGGTTAAC CCTGACTGTG GTCTCAAGAC CCGTAAGTAC 
ACCGAGGTCA AGCCTGCACT CAAGAACATG GTTGATGCGG CTAAGCTCAT CCGCTCCCAG 
CTCGCCAGTG CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_197294.1
CDS	182..2479
Translation	MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLQKVSADLRSSIWKQMSAAGTKFIPSNTFAHYDQVLDTTAMLGAVPPRYGYTGGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYIVPELGPEVNFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKAAKGVDKSFELLSLLPKILPIYKEVITELKAAGATWIQLDEPVLVMDLEGQKLQAFTGAYAELESTLSGLNVLVETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTLDLVKAGFPEGKYLFAGVVDGRNIWANDFAASLSTLQALEGIVGKDKLVVSTSCSLLHTAVDLINETKLDDEIKSWLAFAAQKVVEVNALAKALAGQKDEALFSANAAALASRRSSPRVTNEGVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKVSEEDYVKAIKEEIKKVVDLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKAMTVFWSAMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLAVLEQNILWVNPDCGLKTRKYTEVKPALKNMVDAAKLIRSQLASAK

Target ORF information:

RefSeq Version	NM_121798.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Cobalamin-independent synthase family protein (ATMS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121798.4

ORF Insert Sequence:

ATGGCTTCAC ACATTGTTGG ATACCCACGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TCTGGGATGG TAAGAGCACT GCTGAGGATC TTCAGAAGGT GTCTGCTGAT 
CTCAGGTCAT CCATCTGGAA ACAGATGTCT GCCGCTGGGA CTAAGTTCAT CCCTAGCAAC 
ACCTTTGCTC ACTACGACCA GGTTCTTGAC ACCACCGCCA TGCTCGGTGC TGTTCCACCT 
AGGTATGGAT ACACTGGTGG TGAGATCGGC CTTGATGTTT ACTTCTCCAT GGCTAGAGGA 
AATGCCTCTG TGCCTGCCAT GGAAATGACC AAGTGGTTCG ACACCAACTA CCATTACATC 
GTCCCTGAGT TGGGCCCTGA GGTTAACTTC TCTTACGCAT CCCACAAGGC GGTGAATGAG 
TACAAGGAGG CCAAGGCTCT TGGTGTTGAC ACCGTCCCTG TACTTGTTGG CCCAGTCTCT 
TACTTGCTGC TTTCCAAGGC TGCCAAGGGT GTTGACAAGT CATTCGAACT TCTTTCTCTT 
CTCCCTAAGA TTCTCCCGAT CTACAAGGAA GTGATTACCG AGCTTAAGGC TGCTGGTGCC 
ACCTGGATTC AGCTTGACGA GCCTGTCCTT GTTATGGATC TTGAGGGTCA GAAACTCCAG 
GCCTTTACTG GTGCCTATGC TGAACTTGAA TCAACTCTTT CTGGTTTGAA TGTTCTTGTC 
GAGACCTACT TCGCTGATAT CCCTGCTGAG GCATACAAGA CCCTAACCTC ATTGAAGGGT 
GTGACTGCCT TTGGATTTGA TTTGGTTCGT GGCACCAAGA CCCTTGATTT GGTCAAGGCA 
GGTTTCCCTG AGGGAAAGTA CCTCTTTGCT GGTGTTGTTG ATGGAAGGAA CATCTGGGCC 
AACGACTTTG CTGCGTCCCT AAGCACCTTG CAGGCACTTG AAGGCATTGT TGGTAAAGAC 
AAGCTTGTGG TCTCAACCTC CTGCTCTCTT CTCCACACCG CTGTTGATCT TATCAATGAG 
ACTAAGCTTG ATGATGAAAT CAAGTCATGG TTGGCGTTTG CTGCCCAGAA GGTCGTTGAA 
GTGAACGCTT TGGCCAAGGC TTTGGCTGGT CAGAAGGACG AGGCTCTTTT CTCTGCCAAT 
GCTGCGGCTT TGGCTTCAAG GAGATCTTCC CCAAGAGTCA CCAACGAGGG TGTCCAGAAG 
GCTGCTGCTG CTTTGAAGGG ATCTGACCAC CGTCGTGCAA CCAATGTTAG TGCTAGGCTA 
GATGCTCAGC AGAAGAAGCT CAATCTCCCA ATCCTACCAA CCACAACCAT TGGATCCTTC 
CCACAGACTG TAGAGCTCAG GAGAGTTCGT CGTGAGTACA AGGCCAAAAA GGTCTCAGAG 
GAGGACTACG TTAAAGCCAT CAAGGAAGAG ATCAAGAAAG TTGTTGACCT CCAAGAGGAA 
CTTGACATCG ATGTTCTTGT CCACGGAGAG CCAGAGAGAA ACGACATGGT TGAGTACTTT 
GGTGAGCAGT TGTCTGGTTT TGCCTTCACT GCAAACGGAT GGGTCCAATC TTATGGATCT 
CGCTGTGTGA AGCCACCAGT TATCTATGGT GATGTGAGCC GTCCCAAGGC AATGACCGTC 
TTCTGGTCCG CAATGGCTCA GAGCATGACC TCTCGCCCAA TGAAGGGTAT GCTTACTGGT 
CCCGTCACCA TTCTCAACTG GTCCTTTGTC AGGAACGACC AGCCCAGGCA CGAAACCTGT 
TACCAGATCG CTTTGGCCAT CAAGGACGAA GTCGAGGATC TTGAGAAAGG TGGAATCGGT 
GTCATTCAGA TTGATGAGGC TGCACTTAGA GAAGGACTAC CACTCAGGAA ATCCGAGCAT 
GCTTTCTACT TGGACTGGGC CGTCCACTCC TTCAGAATCA CCAACTGTGG AGTCCAAGAC 
AGCACCCAGA TCCACACTCA CATGTGCTAC TCCCACTTCA ATGACATCAT ACACTCCATC 
ATCGACATGG ATGCTGATGT CATCACCATT GAGAACTCCA GGTCTGATGA GAAGCTTCTT 
TCCGTGTTCC GTGAAGGAGT GAAGTACGGT GCTGGAATCG GTCCAGGAGT CTACGACATC 
CACTCTCCAA GAATACCATC TTCTGAGGAA ATCGCAGACA GGGTCAACAA GATGCTTGCT 
GTCCTAGAGC AGAACATCCT TTGGGTTAAC CCTGACTGTG GTCTCAAGAC CCGTAAGTAC 
ACCGAGGTCA AGCCTGCACT CAAGAACATG GTTGATGCGG CTAAGCTCAT CCGCTCCCAG 
CTCGCCAGTG CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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ATMS1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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