PDAT cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDAT
Entrez Gene ID	831208
Full Name	phospholipid:diacylglycerol acyltransferase
Synonyms	ARABIDOPSIS THALIANA PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE,ATPDAT,PDAT1,PHOSPHOLIPID DIACYLGLYCEROL ACYLTRANSFERASE,PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 1,T6I14.2,phospholipid:diacylglycerol acyltransferase
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_121367.3	NP_196868.1	phospholipid:diacylglycerol acyltransferase
NM_001343283.1	NP_001331240.1	phospholipid:diacylglycerol acyltransferase
NM_001343284.1	NP_001331241.1	phospholipid:diacylglycerol acyltransferase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	LRO1	NP_014405.1
Magnaporthe oryzae (rice blast fungus)	MGG_03560	XP_003716355.1
Eremothecium gossypii	AGOS_AFL179C	NP_985371.1
Schizosaccharomyces pombe (fission yeast)	plh1	NP_596330.2
Neurospora crassa	NCU02416	XP_959598.1
Kluyveromyces lactis	KLLA0F06380g	XP_455369.1
Arabidopsis thaliana (thale cress)	PDAT	NP_196868.1
Oryza sativa (rice)	Os09g0444200	NP_001063298.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE1 (PDAT1) and SUGAR-DEPENDENT1 (SDP1) TAG lipase, function synergistically in directing fatty acids (FAs) toward peroxisomal beta-oxidation via triacylglycerol (TAG) intermediates.
Title: Arabidopsis lipins, PDAT1 acyltransferase, and SDP1 triacylglycerol lipase synergistically direct fatty acids toward ?-oxidation, thereby maintaining membrane lipid homeostasis.

PDAT1 plays a critical role in mediating triacylglycerol (TAG) synthesis and thereby protecting against free fatty acids (FFAs)-induced cell death in fast-growing tissues of plants.
Title: Phospholipid:diacylglycerol acyltransferase-mediated triacylglycerol biosynthesis is crucial for protection against fatty acid-induced cell death in growing tissues of Arabidopsis.

Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2.
Title: Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2.

PDAT1 and DGAT1 have overlapping functions for triacylglycerol synthesis in both seed and pollen, and that the absence of their function leads not only to a reduction in TAG, but also to critical defects in normal pollen and embryo development. [PDAT1]
Title: DGAT1 and PDAT1 acyltransferases have overlapping functions in Arabidopsis triacylglycerol biosynthesis and are essential for normal pollen and seed development.

The following PDAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb40840	NM_001343283.1 Latest version!	Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$342.30 $489.00
OAb18305	NM_121367.3 Latest version!	Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb40839	NM_001343284.1 Latest version!	Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDAT ( NM_001343283.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331240.1 Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.

Price & Availability↑

CloneID	OAb40840
Clone ID Related Accession (Same CDS sequence)	NM_001343283.1
Accession Version	NM_001343283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phospholipid:diacylglycerol acyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGTCACTTG ACAATGAAAC TGGGTTGGAT CCAGCTGGTA TTAGAGTTCG AGCTGTATCA 
GGACTCGTGG CTGCTGACTA CTTTGCTCCT GGCTACTTTG TCTGGGCAGT GCTGATTGCT 
AACCTTGCAC ATATTGGATA TGAAGAGAAA AATATGTACA TGGCTGCATA TGACTGGCGG 
CTTTCGTTTC AGAACACAGA GGTACGTGAT CAGACTCTTA GCCGTATGAA AAGTAATATA 
GAGTTGATGG TTTCTACCAA CGGTGGAAAA AAAGCAGTTA TAGTTCCGCA TTCCATGGGG 
GTCTTGTATT TTCTACATTT TATGAAGTGG GTTGAGGCAC CAGCTCCTCT GGGTGGCGGG 
GGTGGGCCAG ATTGGTGTGC AAAGTATATT AAGGCGGTGA TGAACATTGG TGGACCATTT 
CTTGGTGTTC CAAAAGCTGT TGCAGGGCTT TTCTCTGCTG AAGCAAAGGA TGTTGCAGTT 
GCCAGAGCGA TTGCCCCAGG ATTCTTAGAC ACCGATATAT TTAGACTTCA GACCTTGCAG 
CATGTAATGA GAATGACACG CACATGGGAC TCAACAATGT CTATGTTACC GAAGGGAGGT 
GACACGATAT GGGGCGGGCT TGATTGGTCA CCGGAGAAAG GCCACACCTG TTGTGGGAAA 
AAGCAAAAGA ACAACGAAAC TTGTGGTGAA GCAGGTGAAA ACGGAGTTTC CAAGAAAAGT 
CCTGTTAACT ATGGAAGGAT GATATCTTTT GGGAAAGAAG TAGCAGAGGC TGCGCCATCT 
GAGATTAATA ATATTGATTT TCGAGGTGCT GTCAAAGGTC AGAGTATCCC AAATCACACC 
TGTCGTGACG TGTGGACAGA GTACCATGAC ATGGGAATTG CTGGGATCAA AGCTATCGCT 
GAGTATAAGG TCTACACTGC TGGTGAAGCT ATAGATCTAC TACATTATGT TGCTCCTAAG 
ATGATGGCGC GTGGTGCCGC TCATTTCTCT TATGGAATTG CTGATGATTT GGATGACACC 
AAGTATCAAG ATCCCAAATA CTGGTCAAAT CCGTTAGAGA CAAAATTACC GAATGCTCCT 
GAGATGGAAA TCTACTCATT ATACGGAGTG GGGATACCAA CGGAACGAGC ATACGTATAC 
AAGCTTAACC AGTCTCCCGA CAGTTGCATC CCCTTTCAGA TATTCACTTC TGCTCACGAG 
GAGGACGAAG ATAGCTGTCT GAAAGCAGGA GTTTACAATG TGGATGGGGA TGAAACAGTA 
CCCGTCCTAA GTGCCGGGTA CATGTGTGCA AAAGCGTGGC GTGGCAAGAC AAGATTCAAC 
CCTTCCGGAA TCAAGACTTA TATAAGAGAA TACAATCACT CTCCGCCGGC TAACCTGTTG 
GAAGGGCGCG GGACGCAGAG TGGTGCCCAT GTTGATATCA TGGGAAACTT TGCTTTGATC 
GAAGATATCA TGAGGGTTGC CGCCGGAGGT AACGGGTCTG ATATAGGACA TGACCAGGTC 
CACTCTGGCA TATTTGAATG GTCGGAGCGT ATTGACCTGA AGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331240.1
CDS	1248..2795
Translation	MSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLYFLHFMKWVEAPAPLGGGGGPDWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVARAIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKKQKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSIPNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL

Target ORF information:

RefSeq Version	NM_001343283.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343283.1

ORF Insert Sequence:

ATGTCACTTG ACAATGAAAC TGGGTTGGAT CCAGCTGGTA TTAGAGTTCG AGCTGTATCA 
GGACTCGTGG CTGCTGACTA CTTTGCTCCT GGCTACTTTG TCTGGGCAGT GCTGATTGCT 
AACCTTGCAC ATATTGGATA TGAAGAGAAA AATATGTACA TGGCTGCATA TGACTGGCGG 
CTTTCGTTTC AGAACACAGA GGTACGTGAT CAGACTCTTA GCCGTATGAA AAGTAATATA 
GAGTTGATGG TTTCTACCAA CGGTGGAAAA AAAGCAGTTA TAGTTCCGCA TTCCATGGGG 
GTCTTGTATT TTCTACATTT TATGAAGTGG GTTGAGGCAC CAGCTCCTCT GGGTGGCGGG 
GGTGGGCCAG ATTGGTGTGC AAAGTATATT AAGGCGGTGA TGAACATTGG TGGACCATTT 
CTTGGTGTTC CAAAAGCTGT TGCAGGGCTT TTCTCTGCTG AAGCAAAGGA TGTTGCAGTT 
GCCAGAGCGA TTGCCCCAGG ATTCTTAGAC ACCGATATAT TTAGACTTCA GACCTTGCAG 
CATGTAATGA GAATGACACG CACATGGGAC TCAACAATGT CTATGTTACC GAAGGGAGGT 
GACACGATAT GGGGCGGGCT TGATTGGTCA CCGGAGAAAG GCCACACCTG TTGTGGGAAA 
AAGCAAAAGA ACAACGAAAC TTGTGGTGAA GCAGGTGAAA ACGGAGTTTC CAAGAAAAGT 
CCTGTTAACT ATGGAAGGAT GATATCTTTT GGGAAAGAAG TAGCAGAGGC TGCGCCATCT 
GAGATTAATA ATATTGATTT TCGAGGTGCT GTCAAAGGTC AGAGTATCCC AAATCACACC 
TGTCGTGACG TGTGGACAGA GTACCATGAC ATGGGAATTG CTGGGATCAA AGCTATCGCT 
GAGTATAAGG TCTACACTGC TGGTGAAGCT ATAGATCTAC TACATTATGT TGCTCCTAAG 
ATGATGGCGC GTGGTGCCGC TCATTTCTCT TATGGAATTG CTGATGATTT GGATGACACC 
AAGTATCAAG ATCCCAAATA CTGGTCAAAT CCGTTAGAGA CAAAATTACC GAATGCTCCT 
GAGATGGAAA TCTACTCATT ATACGGAGTG GGGATACCAA CGGAACGAGC ATACGTATAC 
AAGCTTAACC AGTCTCCCGA CAGTTGCATC CCCTTTCAGA TATTCACTTC TGCTCACGAG 
GAGGACGAAG ATAGCTGTCT GAAAGCAGGA GTTTACAATG TGGATGGGGA TGAAACAGTA 
CCCGTCCTAA GTGCCGGGTA CATGTGTGCA AAAGCGTGGC GTGGCAAGAC AAGATTCAAC 
CCTTCCGGAA TCAAGACTTA TATAAGAGAA TACAATCACT CTCCGCCGGC TAACCTGTTG 
GAAGGGCGCG GGACGCAGAG TGGTGCCCAT GTTGATATCA TGGGAAACTT TGCTTTGATC 
GAAGATATCA TGAGGGTTGC CGCCGGAGGT AACGGGTCTG ATATAGGACA TGACCAGGTC 
CACTCTGGCA TATTTGAATG GTCGGAGCGT ATTGACCTGA AGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDAT ( NM_121367.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196868.1 Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.

Price & Availability↑

CloneID	OAb18305
Clone ID Related Accession (Same CDS sequence)	NM_121367.3
Accession Version	NM_121367.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phospholipid:diacylglycerol acyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121367.2.

ATGCCCCTTA TTCATCGGAA AAAGCCGACG GAGAAACCAT CGACGCCGCC ATCTGAAGAG 
GTGGTGCACG ATGAGGATTC GCAAAAGAAA CCACACGAAT CTTCCAAATC CCACCATAAG 
AAATCGAACG GAGGAGGGAA GTGGTCGTGC ATCGATTCTT GTTGTTGGTT CATTGGGTGT 
GTGTGTGTAA CCTGGTGGTT TCTTCTCTTC CTTTACAACG CAATGCCTGC GAGCTTCCCT 
CAGTATGTAA CGGAGCGAAT CACGGGTCCT TTGCCTGACC CGCCCGGTGT TAAGCTCAAA 
AAAGAAGGTC TTAAGGCGAA ACATCCTGTT GTCTTCATTC CTGGGATTGT CACCGGTGGG 
CTCGAGCTTT GGGAAGGCAA ACAATGCGCT GATGGTTTAT TTAGAAAACG TTTGTGGGGT 
GGAACTTTTG GTGAAGTCTA CAAAAGGCCT CTATGTTGGG TGGAACACAT GTCACTTGAC 
AATGAAACTG GGTTGGATCC AGCTGGTATT AGAGTTCGAG CTGTATCAGG ACTCGTGGCT 
GCTGACTACT TTGCTCCTGG CTACTTTGTC TGGGCAGTGC TGATTGCTAA CCTTGCACAT 
ATTGGATATG AAGAGAAAAA TATGTACATG GCTGCATATG ACTGGCGGCT TTCGTTTCAG 
AACACAGAGG TACGTGATCA GACTCTTAGC CGTATGAAAA GTAATATAGA GTTGATGGTT 
TCTACCAACG GTGGAAAAAA AGCAGTTATA GTTCCGCATT CCATGGGGGT CTTGTATTTT 
CTACATTTTA TGAAGTGGGT TGAGGCACCA GCTCCTCTGG GTGGCGGGGG TGGGCCAGAT 
TGGTGTGCAA AGTATATTAA GGCGGTGATG AACATTGGTG GACCATTTCT TGGTGTTCCA 
AAAGCTGTTG CAGGGCTTTT CTCTGCTGAA GCAAAGGATG TTGCAGTTGC CAGAGCGATT 
GCCCCAGGAT TCTTAGACAC CGATATATTT AGACTTCAGA CCTTGCAGCA TGTAATGAGA 
ATGACACGCA CATGGGACTC AACAATGTCT ATGTTACCGA AGGGAGGTGA CACGATATGG 
GGCGGGCTTG ATTGGTCACC GGAGAAAGGC CACACCTGTT GTGGGAAAAA GCAAAAGAAC 
AACGAAACTT GTGGTGAAGC AGGTGAAAAC GGAGTTTCCA AGAAAAGTCC TGTTAACTAT 
GGAAGGATGA TATCTTTTGG GAAAGAAGTA GCAGAGGCTG CGCCATCTGA GATTAATAAT 
ATTGATTTTC GAGGTGCTGT CAAAGGTCAG AGTATCCCAA ATCACACCTG TCGTGACGTG 
TGGACAGAGT ACCATGACAT GGGAATTGCT GGGATCAAAG CTATCGCTGA GTATAAGGTC 
TACACTGCTG GTGAAGCTAT AGATCTACTA CATTATGTTG CTCCTAAGAT GATGGCGCGT 
GGTGCCGCTC ATTTCTCTTA TGGAATTGCT GATGATTTGG ATGACACCAA GTATCAAGAT 
CCCAAATACT GGTCAAATCC GTTAGAGACA AAATTACCGA ATGCTCCTGA GATGGAAATC 
TACTCATTAT ACGGAGTGGG GATACCAACG GAACGAGCAT ACGTATACAA GCTTAACCAG 
TCTCCCGACA GTTGCATCCC CTTTCAGATA TTCACTTCTG CTCACGAGGA GGACGAAGAT 
AGCTGTCTGA AAGCAGGAGT TTACAATGTG GATGGGGATG AAACAGTACC CGTCCTAAGT 
GCCGGGTACA TGTGTGCAAA AGCGTGGCGT GGCAAGACAA GATTCAACCC TTCCGGAATC 
AAGACTTATA TAAGAGAATA CAATCACTCT CCGCCGGCTA ACCTGTTGGA AGGGCGCGGG 
ACGCAGAGTG GTGCCCATGT TGATATCATG GGAAACTTTG CTTTGATCGA AGATATCATG 
AGGGTTGCCG CCGGAGGTAA CGGGTCTGAT ATAGGACATG ACCAGGTCCA CTCTGGCATA 
TTTGAATGGT CGGAGCGTAT TGACCTGAAG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196868.1
CDS	593..2608
Translation	MPLIHRKKPTEKPSTPPSEEVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASFPQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLWGGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLYFLHFMKWVEAPAPLGGGGGPDWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVARAIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKKQKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSIPNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL

Target ORF information:

RefSeq Version	NM_121367.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121367.3

ORF Insert Sequence:

ATGCCCCTTA TTCATCGGAA AAAGCCGACG GAGAAACCAT CGACGCCGCC ATCTGAAGAG 
GTGGTGCACG ATGAGGATTC GCAAAAGAAA CCACACGAAT CTTCCAAATC CCACCATAAG 
AAATCGAACG GAGGAGGGAA GTGGTCGTGC ATCGATTCTT GTTGTTGGTT CATTGGGTGT 
GTGTGTGTAA CCTGGTGGTT TCTTCTCTTC CTTTACAACG CAATGCCTGC GAGCTTCCCT 
CAGTATGTAA CGGAGCGAAT CACGGGTCCT TTGCCTGACC CGCCCGGTGT TAAGCTCAAA 
AAAGAAGGTC TTAAGGCGAA ACATCCTGTT GTCTTCATTC CTGGGATTGT CACCGGTGGG 
CTCGAGCTTT GGGAAGGCAA ACAATGCGCT GATGGTTTAT TTAGAAAACG TTTGTGGGGT 
GGAACTTTTG GTGAAGTCTA CAAAAGGCCT CTATGTTGGG TGGAACACAT GTCACTTGAC 
AATGAAACTG GGTTGGATCC AGCTGGTATT AGAGTTCGAG CTGTATCAGG ACTCGTGGCT 
GCTGACTACT TTGCTCCTGG CTACTTTGTC TGGGCAGTGC TGATTGCTAA CCTTGCACAT 
ATTGGATATG AAGAGAAAAA TATGTACATG GCTGCATATG ACTGGCGGCT TTCGTTTCAG 
AACACAGAGG TACGTGATCA GACTCTTAGC CGTATGAAAA GTAATATAGA GTTGATGGTT 
TCTACCAACG GTGGAAAAAA AGCAGTTATA GTTCCGCATT CCATGGGGGT CTTGTATTTT 
CTACATTTTA TGAAGTGGGT TGAGGCACCA GCTCCTCTGG GTGGCGGGGG TGGGCCAGAT 
TGGTGTGCAA AGTATATTAA GGCGGTGATG AACATTGGTG GACCATTTCT TGGTGTTCCA 
AAAGCTGTTG CAGGGCTTTT CTCTGCTGAA GCAAAGGATG TTGCAGTTGC CAGAGCGATT 
GCCCCAGGAT TCTTAGACAC CGATATATTT AGACTTCAGA CCTTGCAGCA TGTAATGAGA 
ATGACACGCA CATGGGACTC AACAATGTCT ATGTTACCGA AGGGAGGTGA CACGATATGG 
GGCGGGCTTG ATTGGTCACC GGAGAAAGGC CACACCTGTT GTGGGAAAAA GCAAAAGAAC 
AACGAAACTT GTGGTGAAGC AGGTGAAAAC GGAGTTTCCA AGAAAAGTCC TGTTAACTAT 
GGAAGGATGA TATCTTTTGG GAAAGAAGTA GCAGAGGCTG CGCCATCTGA GATTAATAAT 
ATTGATTTTC GAGGTGCTGT CAAAGGTCAG AGTATCCCAA ATCACACCTG TCGTGACGTG 
TGGACAGAGT ACCATGACAT GGGAATTGCT GGGATCAAAG CTATCGCTGA GTATAAGGTC 
TACACTGCTG GTGAAGCTAT AGATCTACTA CATTATGTTG CTCCTAAGAT GATGGCGCGT 
GGTGCCGCTC ATTTCTCTTA TGGAATTGCT GATGATTTGG ATGACACCAA GTATCAAGAT 
CCCAAATACT GGTCAAATCC GTTAGAGACA AAATTACCGA ATGCTCCTGA GATGGAAATC 
TACTCATTAT ACGGAGTGGG GATACCAACG GAACGAGCAT ACGTATACAA GCTTAACCAG 
TCTCCCGACA GTTGCATCCC CTTTCAGATA TTCACTTCTG CTCACGAGGA GGACGAAGAT 
AGCTGTCTGA AAGCAGGAGT TTACAATGTG GATGGGGATG AAACAGTACC CGTCCTAAGT 
GCCGGGTACA TGTGTGCAAA AGCGTGGCGT GGCAAGACAA GATTCAACCC TTCCGGAATC 
AAGACTTATA TAAGAGAATA CAATCACTCT CCGCCGGCTA ACCTGTTGGA AGGGCGCGGG 
ACGCAGAGTG GTGCCCATGT TGATATCATG GGAAACTTTG CTTTGATCGA AGATATCATG 
AGGGTTGCCG CCGGAGGTAA CGGGTCTGAT ATAGGACATG ACCAGGTCCA CTCTGGCATA 
TTTGAATGGT CGGAGCGTAT TGACCTGAAG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDAT ( NM_001343284.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331241.1 Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.

Price & Availability↑

CloneID	OAb40839
Clone ID Related Accession (Same CDS sequence)	NM_001343284.1
Accession Version	NM_001343284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	phospholipid:diacylglycerol acyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGTTTTTGC TATGGCCTCT ATGTTGGGTG GAACACATGT CACTTGACAA TGAAACTGGG 
TTGGATCCAG CTGGTATTAG AGTTCGAGCT GTATCAGGAC TCGTGGCTGC TGACTACTTT 
GCTCCTGGCT ACTTTGTCTG GGCAGTGCTG ATTGCTAACC TTGCACATAT TGGATATGAA 
GAGAAAAATA TGTACATGGC TGCATATGAC TGGCGGCTTT CGTTTCAGAA CACAGAGGTA 
CGTGATCAGA CTCTTAGCCG TATGAAAAGT AATATAGAGT TGATGGTTTC TACCAACGGT 
GGAAAAAAAG CAGTTATAGT TCCGCATTCC ATGGGGGTCT TGTATTTTCT ACATTTTATG 
AAGTGGGTTG AGGCACCAGC TCCTCTGGGT GGCGGGGGTG GGCCAGATTG GTGTGCAAAG 
TATATTAAGG CGGTGATGAA CATTGGTGGA CCATTTCTTG GTGTTCCAAA AGCTGTTGCA 
GGGCTTTTCT CTGCTGAAGC AAAGGATGTT GCAGTTGCCA GAGCGATTGC CCCAGGATTC 
TTAGACACCG ATATATTTAG ACTTCAGACC TTGCAGCATG TAATGAGAAT GACACGCACA 
TGGGACTCAA CAATGTCTAT GTTACCGAAG GGAGGTGACA CGATATGGGG CGGGCTTGAT 
TGGTCACCGG AGAAAGGCCA CACCTGTTGT GGGAAAAAGC AAAAGAACAA CGAAACTTGT 
GGTGAAGCAG GTGAAAACGG AGTTTCCAAG AAAAGTCCTG TTAACTATGG AAGGATGATA 
TCTTTTGGGA AAGAAGTAGC AGAGGCTGCG CCATCTGAGA TTAATAATAT TGATTTTCGA 
GGTGCTGTCA AAGGTCAGAG TATCCCAAAT CACACCTGTC GTGACGTGTG GACAGAGTAC 
CATGACATGG GAATTGCTGG GATCAAAGCT ATCGCTGAGT ATAAGGTCTA CACTGCTGGT 
GAAGCTATAG ATCTACTACA TTATGTTGCT CCTAAGATGA TGGCGCGTGG TGCCGCTCAT 
TTCTCTTATG GAATTGCTGA TGATTTGGAT GACACCAAGT ATCAAGATCC CAAATACTGG 
TCAAATCCGT TAGAGACAAA ATTACCGAAT GCTCCTGAGA TGGAAATCTA CTCATTATAC 
GGAGTGGGGA TACCAACGGA ACGAGCATAC GTATACAAGC TTAACCAGTC TCCCGACAGT 
TGCATCCCCT TTCAGATATT CACTTCTGCT CACGAGGAGG ACGAAGATAG CTGTCTGAAA 
GCAGGAGTTT ACAATGTGGA TGGGGATGAA ACAGTACCCG TCCTAAGTGC CGGGTACATG 
TGTGCAAAAG CGTGGCGTGG CAAGACAAGA TTCAACCCTT CCGGAATCAA GACTTATATA 
AGAGAATACA ATCACTCTCC GCCGGCTAAC CTGTTGGAAG GGCGCGGGAC GCAGAGTGGT 
GCCCATGTTG ATATCATGGG AAACTTTGCT TTGATCGAAG ATATCATGAG GGTTGCCGCC 
GGAGGTAACG GGTCTGATAT AGGACATGAC CAGGTCCACT CTGGCATATT TGAATGGTCG 
GAGCGTATTG ACCTGAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331241.1
CDS	421..2004
Translation	MFLLWPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLYFLHFMKWVEAPAPLGGGGGPDWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVARAIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKKQKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSIPNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL

Target ORF information:

RefSeq Version	NM_001343284.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana phospholipid:diacylglycerol acyltransferase (PDAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343284.1

ORF Insert Sequence:

ATGTTTTTGC TATGGCCTCT ATGTTGGGTG GAACACATGT CACTTGACAA TGAAACTGGG 
TTGGATCCAG CTGGTATTAG AGTTCGAGCT GTATCAGGAC TCGTGGCTGC TGACTACTTT 
GCTCCTGGCT ACTTTGTCTG GGCAGTGCTG ATTGCTAACC TTGCACATAT TGGATATGAA 
GAGAAAAATA TGTACATGGC TGCATATGAC TGGCGGCTTT CGTTTCAGAA CACAGAGGTA 
CGTGATCAGA CTCTTAGCCG TATGAAAAGT AATATAGAGT TGATGGTTTC TACCAACGGT 
GGAAAAAAAG CAGTTATAGT TCCGCATTCC ATGGGGGTCT TGTATTTTCT ACATTTTATG 
AAGTGGGTTG AGGCACCAGC TCCTCTGGGT GGCGGGGGTG GGCCAGATTG GTGTGCAAAG 
TATATTAAGG CGGTGATGAA CATTGGTGGA CCATTTCTTG GTGTTCCAAA AGCTGTTGCA 
GGGCTTTTCT CTGCTGAAGC AAAGGATGTT GCAGTTGCCA GAGCGATTGC CCCAGGATTC 
TTAGACACCG ATATATTTAG ACTTCAGACC TTGCAGCATG TAATGAGAAT GACACGCACA 
TGGGACTCAA CAATGTCTAT GTTACCGAAG GGAGGTGACA CGATATGGGG CGGGCTTGAT 
TGGTCACCGG AGAAAGGCCA CACCTGTTGT GGGAAAAAGC AAAAGAACAA CGAAACTTGT 
GGTGAAGCAG GTGAAAACGG AGTTTCCAAG AAAAGTCCTG TTAACTATGG AAGGATGATA 
TCTTTTGGGA AAGAAGTAGC AGAGGCTGCG CCATCTGAGA TTAATAATAT TGATTTTCGA 
GGTGCTGTCA AAGGTCAGAG TATCCCAAAT CACACCTGTC GTGACGTGTG GACAGAGTAC 
CATGACATGG GAATTGCTGG GATCAAAGCT ATCGCTGAGT ATAAGGTCTA CACTGCTGGT 
GAAGCTATAG ATCTACTACA TTATGTTGCT CCTAAGATGA TGGCGCGTGG TGCCGCTCAT 
TTCTCTTATG GAATTGCTGA TGATTTGGAT GACACCAAGT ATCAAGATCC CAAATACTGG 
TCAAATCCGT TAGAGACAAA ATTACCGAAT GCTCCTGAGA TGGAAATCTA CTCATTATAC 
GGAGTGGGGA TACCAACGGA ACGAGCATAC GTATACAAGC TTAACCAGTC TCCCGACAGT 
TGCATCCCCT TTCAGATATT CACTTCTGCT CACGAGGAGG ACGAAGATAG CTGTCTGAAA 
GCAGGAGTTT ACAATGTGGA TGGGGATGAA ACAGTACCCG TCCTAAGTGC CGGGTACATG 
TGTGCAAAAG CGTGGCGTGG CAAGACAAGA TTCAACCCTT CCGGAATCAA GACTTATATA 
AGAGAATACA ATCACTCTCC GCCGGCTAAC CTGTTGGAAG GGCGCGGGAC GCAGAGTGGT 
GCCCATGTTG ATATCATGGG AAACTTTGCT TTGATCGAAG ATATCATGAG GGTTGCCGCC 
GGAGGTAACG GGTCTGATAT AGGACATGAC CAGGTCCACT CTGGCATATT TGAATGGTCG 
GAGCGTATTG ACCTGAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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PDAT cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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