EMF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMF1
Entrez Gene ID	831025
Full Name	embryonic flower 1 (EMF1)
Synonyms	F15N18.120,F15N18_120,embryonic flower 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001343200.1	NP_001330242.1	embryonic flower 1 (EMF1)
NM_001343201.1	NP_001330243.1	embryonic flower 1 (EMF1)
NM_121191.4	NP_196714.1	embryonic flower 1 (EMF1)

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EMF1 and ULT1 mediate histone mark deposition and modulate transcriptional activities of the target genes.EMF1 and ULT1 act antagonistically on Arabidopsis development and stress response.
Title: EMBRYONIC FLOWER1 and ULTRAPETALA1 Act Antagonistically on Arabidopsis Development and Stress Response.

to promote vegetative development and to regulate cell differentiation during shoot and root organogenesis, EMF1 binds genes required for other developmental phases. These are primarily genes trimethylated by EMF2-PRC2 on their H3K27
Title: EMF1 and PRC2 cooperate to repress key regulators of Arabidopsis development.

EMF1 interacts with EIP1, EIP6 and EIP9 during vegetative development to regulate flowering time in Arabidopsis.
Title: EMF1 interacts with EIP1, EIP6 or EIP9 involved in the regulation of flowering time in Arabidopsis.

EMF1 and EMF2 target transcription factor genes to mediate vegetative development and stress responses, and regulate flowering time genes.
Title: Epigenetic regulation of gene programs by EMF1 and EMF2 in Arabidopsis.

Results are consistent with EMF1's primary role in repressing flowering in order to allow for vegetative growth.
Title: Temporal and spatial requirement of EMF1 activity for Arabidopsis vegetative and reproductive development.

The following EMF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb18208	NM_121191.4 Latest version!	Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb18208	NM_001343201.1 Latest version!	Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb18208	NM_001343200.1 Latest version!	Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMF1 ( NM_001343200.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330242.1 Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.

Price & Availability↑

CloneID	OAb18208
Clone ID Related Accession (Same CDS sequence)	NM_001343200.1 , NM_001343201.1 , NM_121191.4
Accession Version	NM_001343200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	embryonic flower 1 (EMF1)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGGATCTT CCATCAAGAT CAACTCAATA TCCATAGATC TCGCAGGTGC TGCCAACGAG 
ATTGATATGG TGAAATGTGA TCATTTTTCC ATGCGTGGAT TCGTAGCTGA AACTCGTGAG 
AGAGACCTTA GAAAATGTTG GCCGTTTTCA GAAGAGAGTG TTAGTTTAGT AGACCAACAA 
AGCTATACTC TTCCTACTTT ATCTGTTCCA AAGTTTAGAT GGTGGCATTG TATGAGCTGC 
ATCAAAGATA TAGATGCTCA TGGGCCAAAA GATTGTGGAC TGCATTCAAA CTCAAAAGCT 
ATTGGAAACT CTTCTGTTAT CGAAAGTAAA AGCAAGTTCA ATTCGCTAAC TATCATTGAT 
CACGAGAAAG AAAAGAAAAC TGATATTGCA GATAATGCTA TTGAGGAGAA AGTGGGTGTA 
AACTGTGAGA ATGATGATCA GACAGCTACT ACGTTTCTCA AGAAAGCACG TGGTCGACCT 
ATGGGTGCTT CTAATGTTAG GAGCAAGAGC AGAAAGCTTG TGAGTCCGGA GCAGGTAGGA 
AACAACAGAT CTAAAGAAAA ACTAAACAAA CCATCGATGG ATATTAGCAG CTGGAAAGAG 
AAACAAAATG TGGATCAGGC TGTGACAACG TTCGGCTCAT CTGAAATTGC TGGTGTGGTT 
GAGGATACAC CACCTAAGGC AACCAAGAAT CATAAAGGCA TTCGCGGTCT GATGGAATGC 
GATAACGGGT CATCAGAAAG TATAAATCTT GCTATGAGTG GGTTGCAGCG TAGGAAATCT 
CGCAAGGTTC GTCTACTCAG TGAGTTGCTT GGTAATACAA AAACCAGTGG TGGTAGTAAC 
ATCAGAAAAG AAGAGTCTGC TTTGAAGAAG GAATCAGTTA GAGGTCGAAA AAGAAAGTTG 
TTACCTGAAA ACAATTATGT CAGCCGGATA TTGAGTACAA TGGGTGCAAC CTCTGAAAAT 
GCTTCCAAAA GTTGTGACTC TGATCAAGGT AATAGTGAAT CAACTGATAG TGGGTTTGAC 
AGAACTCCAT TTAAGGGTAA GCAGAGAAAC AGAAGATTTC AGGTTGTTGA CGAGTTTGTA 
CCATCACTTC CTTGTGAAAC TTCACAAGAA GGTATCAAGG AGCATGATGC AGATCCTAGT 
AAGAGATCAA CTCCTGCGCA CTCTTTATTC ACTGGAAACG ATTCTGTTCC TTGTCCTCCG 
GGTACTCAGA GAACAGAGAG GAAGCTCAGT TTACCCAAGA AGAAGACAAA GAAGCCTGTA 
ATCGATAATG GGAAGAGCAC TGTGATCAGT TTTAGTAACG GCATTGATGG AAGTCAAGTT 
AACTCGCATA CTGGTCCTTC CATGAACACA GTATCCCAAA CTCGAGACTT ATTGAATGGG 
AAAAGGGTGG GCGGTTTATT TGACAACCGT TTGGCTTCAG ATGGATATTT CAGAAAATAT 
CTCTCTCAGG TTAATGATAA GCCGATAACA TCTTTGCATT TGCAAGACAA TGATTATGTG 
AGGTCAAGAG ACGCGGAACC AAACTGTCTT CGAGATTTTA GTTCCTCTTC TAAATCCAGC 
TCAGGTGGAT GGTTGAGAAC TGGAGTAGAT ATTGTTGACT TCAGAAACAA CAACCACAAT 
ACAAACAGAT CGTCTTTCTC GAACTTGAAG CTAAGATACC CCCCTTCTTC TACTGAAGTT 
GCGGATTTAT CTCGGGTGCT GCAAAAGGAT GCTTCTGGTG CAGATAGAAA GGGGAAGACT 
GTTATGGTCC AAGAACATCA TGGAGCACCA AGAAGCCAAA GTCACGATAG AAAGGAGACT 
ACGACTGAAG AGCAAAACAA CGATGATATT CCAATGGAGA TAGTGGAGCT CATGGCCAAA 
AACCAGTACG AGAGGTGTCT TCCCGACAAA GAAGAAGATG TTAGCAACAA ACAGCCATCA 
CAAGAAACAG CACACAAATC CAAGAATGCT CTACTGATTG ATCTCAATGA AACCTACGAT 
AACGGGATCT CACTTGAGGA CAACAACACA TCAAGACCAC CAAAACCGTG TAGTAGCAAC 
GCAAGGAGGG AAGAACATTT TCCTATGGGA AGACAGCAGA ACTCTCATGA CTTCTTCCCA 
ATAAGTCAGC CTTATGTGCC TTCTCCGTTT GGGATCTTTC CTCCTACCCA AGAAAACCGA 
GCCAGCTCCA TCCGGTTTTC TGGTCACAAC TGTCAGTGGC TTGGGAATTT GCCAACTGTG 
GGTAATCAGA ACCCTTCTCC ATCCTCATTT CGGGTATTAC GTGCTTGTGA TACTTGCCAG 
AGTGTTCCTA ATCAATACAG AGAAGCTTCT CATCCAATTT GGCCATCTTC CATGATACCA 
CCACAGAGTC AATACAAGCC AGTTTCTTTA AATATTAATC AGTCAACAAA TCCGGGTACG 
CTTTCACAGG CATCTAACAA TGAAAATACA TGGAACCTCA ACTTTGTTGC TGCCAACGGG 
AAGCAAAAAT GTGGGCCTAA TCCAGAATTT TCATTTGGCT GCAAACATGC TGCTGGGGTT 
AGTAGTAGTA GTAGTAGGCC AATAGATAAC TTTTCTAGTG AGAGCTCTAT ACCGGCATTG 
CATCTACTCA GCCTTCTGGA TCCTCGCCTG AGGTCAACGA CTCCCGCTGA CCAACACGGA 
AACACTAAAT TTACTAAAAG ACATTTTCCG CCAGCCAACC AGTCCAAAGA GTTTATAGAG 
CTTCAAACAG GGGACTCTAG TAAGTCAGCC TACTCAACTA AGCAGATACC TTTTGATCTA 
TACAGCAAGA GATTCACACA AGAGCCTTCC CGGAAGAGTT TCCCCATCAC TCCACCTATT 
GGGACGTCTT CACTTTCATT TCAAAATGCT TCATGGAGTC CTCATCATCA AGAGAAGAAA 
ACCAAGAGAA AAGACACCTT TGCTCCTGTT TACAATACTC ATGAAAAGCC GGTGTTTGCA 
AGCAGCAACG ACCAAGCGAA GTTCCAGCTG CTGGGAGCAT CGAATTCCAT GATGCTTCCT 
TTGAAATTTC ACATGACGGA TAAAGAAAAG AAACAAAAGA GAAAAGCAGA GAGCTGCAAT 
AACAATGCCT CTGCGGGACC TGTGAAGAAC AGTTCTGGAC CCATTGTGTG CAGCGTCAAT 
AGAAACCCTG CTGATTTCAC CATTCCTGAA CCTGGGAATG TTTACATGTT AACGGGTGAA 
CATCTGAAAG TCCGTAAACG CACAACCTTC AAGAAAAAGC CAGCCGTGTG TAAGCAGGAC 
GCAATGAAGC AGACCAAGAA GCCGGTTTGT CCACCTACAC AAAATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330242.1
CDS	703..3993
Translation	MGSSIKINSISIDLAGAANEIDMVKCDHFSMRGFVAETRERDLRKCWPFSEESVSLVDQQSYTLPTLSVPKFRWWHCMSCIKDIDAHGPKDCGLHSNSKAIGNSSVIESKSKFNSLTIIDHEKEKKTDIADNAIEEKVGVNCENDDQTATTFLKKARGRPMGASNVRSKSRKLVSPEQVGNNRSKEKLNKPSMDISSWKEKQNVDQAVTTFGSSEIAGVVEDTPPKATKNHKGIRGLMECDNGSSESINLAMSGLQRRKSRKVRLLSELLGNTKTSGGSNIRKEESALKKESVRGRKRKLLPENNYVSRILSTMGATSENASKSCDSDQGNSESTDSGFDRTPFKGKQRNRRFQVVDEFVPSLPCETSQEGIKEHDADPSKRSTPAHSLFTGNDSVPCPPGTQRTERKLSLPKKKTKKPVIDNGKSTVISFSNGIDGSQVNSHTGPSMNTVSQTRDLLNGKRVGGLFDNRLASDGYFRKYLSQVNDKPITSLHLQDNDYVRSRDAEPNCLRDFSSSSKSSSGGWLRTGVDIVDFRNNNHNTNRSSFSNLKLRYPPSSTEVADLSRVLQKDASGADRKGKTVMVQEHHGAPRSQSHDRKETTTEEQNNDDIPMEIVELMAKNQYERCLPDKEEDVSNKQPSQETAHKSKNALLIDLNETYDNGISLEDNNTSRPPKPCSSNARREEHFPMGRQQNSHDFFPISQPYVPSPFGIFPPTQENRASSIRFSGHNCQWLGNLPTVGNQNPSPSSFRVLRACDTCQSVPNQYREASHPIWPSSMIPPQSQYKPVSLNINQSTNPGTLSQASNNENTWNLNFVAANGKQKCGPNPEFSFGCKHAAGVSSSSSRPIDNFSSESSIPALHLLSLLDPRLRSTTPADQHGNTKFTKRHFPPANQSKEFIELQTGDSSKSAYSTKQIPFDLYSKRFTQEPSRKSFPITPPIGTSSLSFQNASWSPHHQEKKTKRKDTFAPVYNTHEKPVFASSNDQAKFQLLGASNSMMLPLKFHMTDKEKKQKRKAESCNNNASAGPVKNSSGPIVCSVNRNPADFTIPEPGNVYMLTGEHLKVRKRTTFKKKPAVCKQDAMKQTKKPVCPPTQNA

Target ORF information:

RefSeq Version	NM_001343200.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343200.1

ORF Insert Sequence:

ATGGGATCTT CCATCAAGAT CAACTCAATA TCCATAGATC TCGCAGGTGC TGCCAACGAG 
ATTGATATGG TGAAATGTGA TCATTTTTCC ATGCGTGGAT TCGTAGCTGA AACTCGTGAG 
AGAGACCTTA GAAAATGTTG GCCGTTTTCA GAAGAGAGTG TTAGTTTAGT AGACCAACAA 
AGCTATACTC TTCCTACTTT ATCTGTTCCA AAGTTTAGAT GGTGGCATTG TATGAGCTGC 
ATCAAAGATA TAGATGCTCA TGGGCCAAAA GATTGTGGAC TGCATTCAAA CTCAAAAGCT 
ATTGGAAACT CTTCTGTTAT CGAAAGTAAA AGCAAGTTCA ATTCGCTAAC TATCATTGAT 
CACGAGAAAG AAAAGAAAAC TGATATTGCA GATAATGCTA TTGAGGAGAA AGTGGGTGTA 
AACTGTGAGA ATGATGATCA GACAGCTACT ACGTTTCTCA AGAAAGCACG TGGTCGACCT 
ATGGGTGCTT CTAATGTTAG GAGCAAGAGC AGAAAGCTTG TGAGTCCGGA GCAGGTAGGA 
AACAACAGAT CTAAAGAAAA ACTAAACAAA CCATCGATGG ATATTAGCAG CTGGAAAGAG 
AAACAAAATG TGGATCAGGC TGTGACAACG TTCGGCTCAT CTGAAATTGC TGGTGTGGTT 
GAGGATACAC CACCTAAGGC AACCAAGAAT CATAAAGGCA TTCGCGGTCT GATGGAATGC 
GATAACGGGT CATCAGAAAG TATAAATCTT GCTATGAGTG GGTTGCAGCG TAGGAAATCT 
CGCAAGGTTC GTCTACTCAG TGAGTTGCTT GGTAATACAA AAACCAGTGG TGGTAGTAAC 
ATCAGAAAAG AAGAGTCTGC TTTGAAGAAG GAATCAGTTA GAGGTCGAAA AAGAAAGTTG 
TTACCTGAAA ACAATTATGT CAGCCGGATA TTGAGTACAA TGGGTGCAAC CTCTGAAAAT 
GCTTCCAAAA GTTGTGACTC TGATCAAGGT AATAGTGAAT CAACTGATAG TGGGTTTGAC 
AGAACTCCAT TTAAGGGTAA GCAGAGAAAC AGAAGATTTC AGGTTGTTGA CGAGTTTGTA 
CCATCACTTC CTTGTGAAAC TTCACAAGAA GGTATCAAGG AGCATGATGC AGATCCTAGT 
AAGAGATCAA CTCCTGCGCA CTCTTTATTC ACTGGAAACG ATTCTGTTCC TTGTCCTCCG 
GGTACTCAGA GAACAGAGAG GAAGCTCAGT TTACCCAAGA AGAAGACAAA GAAGCCTGTA 
ATCGATAATG GGAAGAGCAC TGTGATCAGT TTTAGTAACG GCATTGATGG AAGTCAAGTT 
AACTCGCATA CTGGTCCTTC CATGAACACA GTATCCCAAA CTCGAGACTT ATTGAATGGG 
AAAAGGGTGG GCGGTTTATT TGACAACCGT TTGGCTTCAG ATGGATATTT CAGAAAATAT 
CTCTCTCAGG TTAATGATAA GCCGATAACA TCTTTGCATT TGCAAGACAA TGATTATGTG 
AGGTCAAGAG ACGCGGAACC AAACTGTCTT CGAGATTTTA GTTCCTCTTC TAAATCCAGC 
TCAGGTGGAT GGTTGAGAAC TGGAGTAGAT ATTGTTGACT TCAGAAACAA CAACCACAAT 
ACAAACAGAT CGTCTTTCTC GAACTTGAAG CTAAGATACC CCCCTTCTTC TACTGAAGTT 
GCGGATTTAT CTCGGGTGCT GCAAAAGGAT GCTTCTGGTG CAGATAGAAA GGGGAAGACT 
GTTATGGTCC AAGAACATCA TGGAGCACCA AGAAGCCAAA GTCACGATAG AAAGGAGACT 
ACGACTGAAG AGCAAAACAA CGATGATATT CCAATGGAGA TAGTGGAGCT CATGGCCAAA 
AACCAGTACG AGAGGTGTCT TCCCGACAAA GAAGAAGATG TTAGCAACAA ACAGCCATCA 
CAAGAAACAG CACACAAATC CAAGAATGCT CTACTGATTG ATCTCAATGA AACCTACGAT 
AACGGGATCT CACTTGAGGA CAACAACACA TCAAGACCAC CAAAACCGTG TAGTAGCAAC 
GCAAGGAGGG AAGAACATTT TCCTATGGGA AGACAGCAGA ACTCTCATGA CTTCTTCCCA 
ATAAGTCAGC CTTATGTGCC TTCTCCGTTT GGGATCTTTC CTCCTACCCA AGAAAACCGA 
GCCAGCTCCA TCCGGTTTTC TGGTCACAAC TGTCAGTGGC TTGGGAATTT GCCAACTGTG 
GGTAATCAGA ACCCTTCTCC ATCCTCATTT CGGGTATTAC GTGCTTGTGA TACTTGCCAG 
AGTGTTCCTA ATCAATACAG AGAAGCTTCT CATCCAATTT GGCCATCTTC CATGATACCA 
CCACAGAGTC AATACAAGCC AGTTTCTTTA AATATTAATC AGTCAACAAA TCCGGGTACG 
CTTTCACAGG CATCTAACAA TGAAAATACA TGGAACCTCA ACTTTGTTGC TGCCAACGGG 
AAGCAAAAAT GTGGGCCTAA TCCAGAATTT TCATTTGGCT GCAAACATGC TGCTGGGGTT 
AGTAGTAGTA GTAGTAGGCC AATAGATAAC TTTTCTAGTG AGAGCTCTAT ACCGGCATTG 
CATCTACTCA GCCTTCTGGA TCCTCGCCTG AGGTCAACGA CTCCCGCTGA CCAACACGGA 
AACACTAAAT TTACTAAAAG ACATTTTCCG CCAGCCAACC AGTCCAAAGA GTTTATAGAG 
CTTCAAACAG GGGACTCTAG TAAGTCAGCC TACTCAACTA AGCAGATACC TTTTGATCTA 
TACAGCAAGA GATTCACACA AGAGCCTTCC CGGAAGAGTT TCCCCATCAC TCCACCTATT 
GGGACGTCTT CACTTTCATT TCAAAATGCT TCATGGAGTC CTCATCATCA AGAGAAGAAA 
ACCAAGAGAA AAGACACCTT TGCTCCTGTT TACAATACTC ATGAAAAGCC GGTGTTTGCA 
AGCAGCAACG ACCAAGCGAA GTTCCAGCTG CTGGGAGCAT CGAATTCCAT GATGCTTCCT 
TTGAAATTTC ACATGACGGA TAAAGAAAAG AAACAAAAGA GAAAAGCAGA GAGCTGCAAT 
AACAATGCCT CTGCGGGACC TGTGAAGAAC AGTTCTGGAC CCATTGTGTG CAGCGTCAAT 
AGAAACCCTG CTGATTTCAC CATTCCTGAA CCTGGGAATG TTTACATGTT AACGGGTGAA 
CATCTGAAAG TCCGTAAACG CACAACCTTC AAGAAAAAGC CAGCCGTGTG TAAGCAGGAC 
GCAATGAAGC AGACCAAGAA GCCGGTTTGT CCACCTACAC AAAATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMF1 ( NM_001343201.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330243.1 Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.

Price & Availability↑

CloneID	OAb18208
Clone ID Related Accession (Same CDS sequence)	NM_001343200.1 , NM_001343201.1 , NM_121191.4
Accession Version	NM_001343201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	embryonic flower 1 (EMF1)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGGATCTT CCATCAAGAT CAACTCAATA TCCATAGATC TCGCAGGTGC TGCCAACGAG 
ATTGATATGG TGAAATGTGA TCATTTTTCC ATGCGTGGAT TCGTAGCTGA AACTCGTGAG 
AGAGACCTTA GAAAATGTTG GCCGTTTTCA GAAGAGAGTG TTAGTTTAGT AGACCAACAA 
AGCTATACTC TTCCTACTTT ATCTGTTCCA AAGTTTAGAT GGTGGCATTG TATGAGCTGC 
ATCAAAGATA TAGATGCTCA TGGGCCAAAA GATTGTGGAC TGCATTCAAA CTCAAAAGCT 
ATTGGAAACT CTTCTGTTAT CGAAAGTAAA AGCAAGTTCA ATTCGCTAAC TATCATTGAT 
CACGAGAAAG AAAAGAAAAC TGATATTGCA GATAATGCTA TTGAGGAGAA AGTGGGTGTA 
AACTGTGAGA ATGATGATCA GACAGCTACT ACGTTTCTCA AGAAAGCACG TGGTCGACCT 
ATGGGTGCTT CTAATGTTAG GAGCAAGAGC AGAAAGCTTG TGAGTCCGGA GCAGGTAGGA 
AACAACAGAT CTAAAGAAAA ACTAAACAAA CCATCGATGG ATATTAGCAG CTGGAAAGAG 
AAACAAAATG TGGATCAGGC TGTGACAACG TTCGGCTCAT CTGAAATTGC TGGTGTGGTT 
GAGGATACAC CACCTAAGGC AACCAAGAAT CATAAAGGCA TTCGCGGTCT GATGGAATGC 
GATAACGGGT CATCAGAAAG TATAAATCTT GCTATGAGTG GGTTGCAGCG TAGGAAATCT 
CGCAAGGTTC GTCTACTCAG TGAGTTGCTT GGTAATACAA AAACCAGTGG TGGTAGTAAC 
ATCAGAAAAG AAGAGTCTGC TTTGAAGAAG GAATCAGTTA GAGGTCGAAA AAGAAAGTTG 
TTACCTGAAA ACAATTATGT CAGCCGGATA TTGAGTACAA TGGGTGCAAC CTCTGAAAAT 
GCTTCCAAAA GTTGTGACTC TGATCAAGGT AATAGTGAAT CAACTGATAG TGGGTTTGAC 
AGAACTCCAT TTAAGGGTAA GCAGAGAAAC AGAAGATTTC AGGTTGTTGA CGAGTTTGTA 
CCATCACTTC CTTGTGAAAC TTCACAAGAA GGTATCAAGG AGCATGATGC AGATCCTAGT 
AAGAGATCAA CTCCTGCGCA CTCTTTATTC ACTGGAAACG ATTCTGTTCC TTGTCCTCCG 
GGTACTCAGA GAACAGAGAG GAAGCTCAGT TTACCCAAGA AGAAGACAAA GAAGCCTGTA 
ATCGATAATG GGAAGAGCAC TGTGATCAGT TTTAGTAACG GCATTGATGG AAGTCAAGTT 
AACTCGCATA CTGGTCCTTC CATGAACACA GTATCCCAAA CTCGAGACTT ATTGAATGGG 
AAAAGGGTGG GCGGTTTATT TGACAACCGT TTGGCTTCAG ATGGATATTT CAGAAAATAT 
CTCTCTCAGG TTAATGATAA GCCGATAACA TCTTTGCATT TGCAAGACAA TGATTATGTG 
AGGTCAAGAG ACGCGGAACC AAACTGTCTT CGAGATTTTA GTTCCTCTTC TAAATCCAGC 
TCAGGTGGAT GGTTGAGAAC TGGAGTAGAT ATTGTTGACT TCAGAAACAA CAACCACAAT 
ACAAACAGAT CGTCTTTCTC GAACTTGAAG CTAAGATACC CCCCTTCTTC TACTGAAGTT 
GCGGATTTAT CTCGGGTGCT GCAAAAGGAT GCTTCTGGTG CAGATAGAAA GGGGAAGACT 
GTTATGGTCC AAGAACATCA TGGAGCACCA AGAAGCCAAA GTCACGATAG AAAGGAGACT 
ACGACTGAAG AGCAAAACAA CGATGATATT CCAATGGAGA TAGTGGAGCT CATGGCCAAA 
AACCAGTACG AGAGGTGTCT TCCCGACAAA GAAGAAGATG TTAGCAACAA ACAGCCATCA 
CAAGAAACAG CACACAAATC CAAGAATGCT CTACTGATTG ATCTCAATGA AACCTACGAT 
AACGGGATCT CACTTGAGGA CAACAACACA TCAAGACCAC CAAAACCGTG TAGTAGCAAC 
GCAAGGAGGG AAGAACATTT TCCTATGGGA AGACAGCAGA ACTCTCATGA CTTCTTCCCA 
ATAAGTCAGC CTTATGTGCC TTCTCCGTTT GGGATCTTTC CTCCTACCCA AGAAAACCGA 
GCCAGCTCCA TCCGGTTTTC TGGTCACAAC TGTCAGTGGC TTGGGAATTT GCCAACTGTG 
GGTAATCAGA ACCCTTCTCC ATCCTCATTT CGGGTATTAC GTGCTTGTGA TACTTGCCAG 
AGTGTTCCTA ATCAATACAG AGAAGCTTCT CATCCAATTT GGCCATCTTC CATGATACCA 
CCACAGAGTC AATACAAGCC AGTTTCTTTA AATATTAATC AGTCAACAAA TCCGGGTACG 
CTTTCACAGG CATCTAACAA TGAAAATACA TGGAACCTCA ACTTTGTTGC TGCCAACGGG 
AAGCAAAAAT GTGGGCCTAA TCCAGAATTT TCATTTGGCT GCAAACATGC TGCTGGGGTT 
AGTAGTAGTA GTAGTAGGCC AATAGATAAC TTTTCTAGTG AGAGCTCTAT ACCGGCATTG 
CATCTACTCA GCCTTCTGGA TCCTCGCCTG AGGTCAACGA CTCCCGCTGA CCAACACGGA 
AACACTAAAT TTACTAAAAG ACATTTTCCG CCAGCCAACC AGTCCAAAGA GTTTATAGAG 
CTTCAAACAG GGGACTCTAG TAAGTCAGCC TACTCAACTA AGCAGATACC TTTTGATCTA 
TACAGCAAGA GATTCACACA AGAGCCTTCC CGGAAGAGTT TCCCCATCAC TCCACCTATT 
GGGACGTCTT CACTTTCATT TCAAAATGCT TCATGGAGTC CTCATCATCA AGAGAAGAAA 
ACCAAGAGAA AAGACACCTT TGCTCCTGTT TACAATACTC ATGAAAAGCC GGTGTTTGCA 
AGCAGCAACG ACCAAGCGAA GTTCCAGCTG CTGGGAGCAT CGAATTCCAT GATGCTTCCT 
TTGAAATTTC ACATGACGGA TAAAGAAAAG AAACAAAAGA GAAAAGCAGA GAGCTGCAAT 
AACAATGCCT CTGCGGGACC TGTGAAGAAC AGTTCTGGAC CCATTGTGTG CAGCGTCAAT 
AGAAACCCTG CTGATTTCAC CATTCCTGAA CCTGGGAATG TTTACATGTT AACGGGTGAA 
CATCTGAAAG TCCGTAAACG CACAACCTTC AAGAAAAAGC CAGCCGTGTG TAAGCAGGAC 
GCAATGAAGC AGACCAAGAA GCCGGTTTGT CCACCTACAC AAAATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330243.1
CDS	425..3715
Translation	MGSSIKINSISIDLAGAANEIDMVKCDHFSMRGFVAETRERDLRKCWPFSEESVSLVDQQSYTLPTLSVPKFRWWHCMSCIKDIDAHGPKDCGLHSNSKAIGNSSVIESKSKFNSLTIIDHEKEKKTDIADNAIEEKVGVNCENDDQTATTFLKKARGRPMGASNVRSKSRKLVSPEQVGNNRSKEKLNKPSMDISSWKEKQNVDQAVTTFGSSEIAGVVEDTPPKATKNHKGIRGLMECDNGSSESINLAMSGLQRRKSRKVRLLSELLGNTKTSGGSNIRKEESALKKESVRGRKRKLLPENNYVSRILSTMGATSENASKSCDSDQGNSESTDSGFDRTPFKGKQRNRRFQVVDEFVPSLPCETSQEGIKEHDADPSKRSTPAHSLFTGNDSVPCPPGTQRTERKLSLPKKKTKKPVIDNGKSTVISFSNGIDGSQVNSHTGPSMNTVSQTRDLLNGKRVGGLFDNRLASDGYFRKYLSQVNDKPITSLHLQDNDYVRSRDAEPNCLRDFSSSSKSSSGGWLRTGVDIVDFRNNNHNTNRSSFSNLKLRYPPSSTEVADLSRVLQKDASGADRKGKTVMVQEHHGAPRSQSHDRKETTTEEQNNDDIPMEIVELMAKNQYERCLPDKEEDVSNKQPSQETAHKSKNALLIDLNETYDNGISLEDNNTSRPPKPCSSNARREEHFPMGRQQNSHDFFPISQPYVPSPFGIFPPTQENRASSIRFSGHNCQWLGNLPTVGNQNPSPSSFRVLRACDTCQSVPNQYREASHPIWPSSMIPPQSQYKPVSLNINQSTNPGTLSQASNNENTWNLNFVAANGKQKCGPNPEFSFGCKHAAGVSSSSSRPIDNFSSESSIPALHLLSLLDPRLRSTTPADQHGNTKFTKRHFPPANQSKEFIELQTGDSSKSAYSTKQIPFDLYSKRFTQEPSRKSFPITPPIGTSSLSFQNASWSPHHQEKKTKRKDTFAPVYNTHEKPVFASSNDQAKFQLLGASNSMMLPLKFHMTDKEKKQKRKAESCNNNASAGPVKNSSGPIVCSVNRNPADFTIPEPGNVYMLTGEHLKVRKRTTFKKKPAVCKQDAMKQTKKPVCPPTQNA

Target ORF information:

RefSeq Version	NM_001343201.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001343201.1

ORF Insert Sequence:

ATGGGATCTT CCATCAAGAT CAACTCAATA TCCATAGATC TCGCAGGTGC TGCCAACGAG 
ATTGATATGG TGAAATGTGA TCATTTTTCC ATGCGTGGAT TCGTAGCTGA AACTCGTGAG 
AGAGACCTTA GAAAATGTTG GCCGTTTTCA GAAGAGAGTG TTAGTTTAGT AGACCAACAA 
AGCTATACTC TTCCTACTTT ATCTGTTCCA AAGTTTAGAT GGTGGCATTG TATGAGCTGC 
ATCAAAGATA TAGATGCTCA TGGGCCAAAA GATTGTGGAC TGCATTCAAA CTCAAAAGCT 
ATTGGAAACT CTTCTGTTAT CGAAAGTAAA AGCAAGTTCA ATTCGCTAAC TATCATTGAT 
CACGAGAAAG AAAAGAAAAC TGATATTGCA GATAATGCTA TTGAGGAGAA AGTGGGTGTA 
AACTGTGAGA ATGATGATCA GACAGCTACT ACGTTTCTCA AGAAAGCACG TGGTCGACCT 
ATGGGTGCTT CTAATGTTAG GAGCAAGAGC AGAAAGCTTG TGAGTCCGGA GCAGGTAGGA 
AACAACAGAT CTAAAGAAAA ACTAAACAAA CCATCGATGG ATATTAGCAG CTGGAAAGAG 
AAACAAAATG TGGATCAGGC TGTGACAACG TTCGGCTCAT CTGAAATTGC TGGTGTGGTT 
GAGGATACAC CACCTAAGGC AACCAAGAAT CATAAAGGCA TTCGCGGTCT GATGGAATGC 
GATAACGGGT CATCAGAAAG TATAAATCTT GCTATGAGTG GGTTGCAGCG TAGGAAATCT 
CGCAAGGTTC GTCTACTCAG TGAGTTGCTT GGTAATACAA AAACCAGTGG TGGTAGTAAC 
ATCAGAAAAG AAGAGTCTGC TTTGAAGAAG GAATCAGTTA GAGGTCGAAA AAGAAAGTTG 
TTACCTGAAA ACAATTATGT CAGCCGGATA TTGAGTACAA TGGGTGCAAC CTCTGAAAAT 
GCTTCCAAAA GTTGTGACTC TGATCAAGGT AATAGTGAAT CAACTGATAG TGGGTTTGAC 
AGAACTCCAT TTAAGGGTAA GCAGAGAAAC AGAAGATTTC AGGTTGTTGA CGAGTTTGTA 
CCATCACTTC CTTGTGAAAC TTCACAAGAA GGTATCAAGG AGCATGATGC AGATCCTAGT 
AAGAGATCAA CTCCTGCGCA CTCTTTATTC ACTGGAAACG ATTCTGTTCC TTGTCCTCCG 
GGTACTCAGA GAACAGAGAG GAAGCTCAGT TTACCCAAGA AGAAGACAAA GAAGCCTGTA 
ATCGATAATG GGAAGAGCAC TGTGATCAGT TTTAGTAACG GCATTGATGG AAGTCAAGTT 
AACTCGCATA CTGGTCCTTC CATGAACACA GTATCCCAAA CTCGAGACTT ATTGAATGGG 
AAAAGGGTGG GCGGTTTATT TGACAACCGT TTGGCTTCAG ATGGATATTT CAGAAAATAT 
CTCTCTCAGG TTAATGATAA GCCGATAACA TCTTTGCATT TGCAAGACAA TGATTATGTG 
AGGTCAAGAG ACGCGGAACC AAACTGTCTT CGAGATTTTA GTTCCTCTTC TAAATCCAGC 
TCAGGTGGAT GGTTGAGAAC TGGAGTAGAT ATTGTTGACT TCAGAAACAA CAACCACAAT 
ACAAACAGAT CGTCTTTCTC GAACTTGAAG CTAAGATACC CCCCTTCTTC TACTGAAGTT 
GCGGATTTAT CTCGGGTGCT GCAAAAGGAT GCTTCTGGTG CAGATAGAAA GGGGAAGACT 
GTTATGGTCC AAGAACATCA TGGAGCACCA AGAAGCCAAA GTCACGATAG AAAGGAGACT 
ACGACTGAAG AGCAAAACAA CGATGATATT CCAATGGAGA TAGTGGAGCT CATGGCCAAA 
AACCAGTACG AGAGGTGTCT TCCCGACAAA GAAGAAGATG TTAGCAACAA ACAGCCATCA 
CAAGAAACAG CACACAAATC CAAGAATGCT CTACTGATTG ATCTCAATGA AACCTACGAT 
AACGGGATCT CACTTGAGGA CAACAACACA TCAAGACCAC CAAAACCGTG TAGTAGCAAC 
GCAAGGAGGG AAGAACATTT TCCTATGGGA AGACAGCAGA ACTCTCATGA CTTCTTCCCA 
ATAAGTCAGC CTTATGTGCC TTCTCCGTTT GGGATCTTTC CTCCTACCCA AGAAAACCGA 
GCCAGCTCCA TCCGGTTTTC TGGTCACAAC TGTCAGTGGC TTGGGAATTT GCCAACTGTG 
GGTAATCAGA ACCCTTCTCC ATCCTCATTT CGGGTATTAC GTGCTTGTGA TACTTGCCAG 
AGTGTTCCTA ATCAATACAG AGAAGCTTCT CATCCAATTT GGCCATCTTC CATGATACCA 
CCACAGAGTC AATACAAGCC AGTTTCTTTA AATATTAATC AGTCAACAAA TCCGGGTACG 
CTTTCACAGG CATCTAACAA TGAAAATACA TGGAACCTCA ACTTTGTTGC TGCCAACGGG 
AAGCAAAAAT GTGGGCCTAA TCCAGAATTT TCATTTGGCT GCAAACATGC TGCTGGGGTT 
AGTAGTAGTA GTAGTAGGCC AATAGATAAC TTTTCTAGTG AGAGCTCTAT ACCGGCATTG 
CATCTACTCA GCCTTCTGGA TCCTCGCCTG AGGTCAACGA CTCCCGCTGA CCAACACGGA 
AACACTAAAT TTACTAAAAG ACATTTTCCG CCAGCCAACC AGTCCAAAGA GTTTATAGAG 
CTTCAAACAG GGGACTCTAG TAAGTCAGCC TACTCAACTA AGCAGATACC TTTTGATCTA 
TACAGCAAGA GATTCACACA AGAGCCTTCC CGGAAGAGTT TCCCCATCAC TCCACCTATT 
GGGACGTCTT CACTTTCATT TCAAAATGCT TCATGGAGTC CTCATCATCA AGAGAAGAAA 
ACCAAGAGAA AAGACACCTT TGCTCCTGTT TACAATACTC ATGAAAAGCC GGTGTTTGCA 
AGCAGCAACG ACCAAGCGAA GTTCCAGCTG CTGGGAGCAT CGAATTCCAT GATGCTTCCT 
TTGAAATTTC ACATGACGGA TAAAGAAAAG AAACAAAAGA GAAAAGCAGA GAGCTGCAAT 
AACAATGCCT CTGCGGGACC TGTGAAGAAC AGTTCTGGAC CCATTGTGTG CAGCGTCAAT 
AGAAACCCTG CTGATTTCAC CATTCCTGAA CCTGGGAATG TTTACATGTT AACGGGTGAA 
CATCTGAAAG TCCGTAAACG CACAACCTTC AAGAAAAAGC CAGCCGTGTG TAAGCAGGAC 
GCAATGAAGC AGACCAAGAA GCCGGTTTGT CCACCTACAC AAAATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMF1 ( NM_121191.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196714.1 Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.

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CloneID	OAb18208
Clone ID Related Accession (Same CDS sequence)	NM_001343200.1 , NM_001343201.1 , NM_121191.4
Accession Version	NM_121191.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	embryonic flower 1 (EMF1)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_121191.3.

ATGGGATCTT CCATCAAGAT CAACTCAATA TCCATAGATC TCGCAGGTGC TGCCAACGAG 
ATTGATATGG TGAAATGTGA TCATTTTTCC ATGCGTGGAT TCGTAGCTGA AACTCGTGAG 
AGAGACCTTA GAAAATGTTG GCCGTTTTCA GAAGAGAGTG TTAGTTTAGT AGACCAACAA 
AGCTATACTC TTCCTACTTT ATCTGTTCCA AAGTTTAGAT GGTGGCATTG TATGAGCTGC 
ATCAAAGATA TAGATGCTCA TGGGCCAAAA GATTGTGGAC TGCATTCAAA CTCAAAAGCT 
ATTGGAAACT CTTCTGTTAT CGAAAGTAAA AGCAAGTTCA ATTCGCTAAC TATCATTGAT 
CACGAGAAAG AAAAGAAAAC TGATATTGCA GATAATGCTA TTGAGGAGAA AGTGGGTGTA 
AACTGTGAGA ATGATGATCA GACAGCTACT ACGTTTCTCA AGAAAGCACG TGGTCGACCT 
ATGGGTGCTT CTAATGTTAG GAGCAAGAGC AGAAAGCTTG TGAGTCCGGA GCAGGTAGGA 
AACAACAGAT CTAAAGAAAA ACTAAACAAA CCATCGATGG ATATTAGCAG CTGGAAAGAG 
AAACAAAATG TGGATCAGGC TGTGACAACG TTCGGCTCAT CTGAAATTGC TGGTGTGGTT 
GAGGATACAC CACCTAAGGC AACCAAGAAT CATAAAGGCA TTCGCGGTCT GATGGAATGC 
GATAACGGGT CATCAGAAAG TATAAATCTT GCTATGAGTG GGTTGCAGCG TAGGAAATCT 
CGCAAGGTTC GTCTACTCAG TGAGTTGCTT GGTAATACAA AAACCAGTGG TGGTAGTAAC 
ATCAGAAAAG AAGAGTCTGC TTTGAAGAAG GAATCAGTTA GAGGTCGAAA AAGAAAGTTG 
TTACCTGAAA ACAATTATGT CAGCCGGATA TTGAGTACAA TGGGTGCAAC CTCTGAAAAT 
GCTTCCAAAA GTTGTGACTC TGATCAAGGT AATAGTGAAT CAACTGATAG TGGGTTTGAC 
AGAACTCCAT TTAAGGGTAA GCAGAGAAAC AGAAGATTTC AGGTTGTTGA CGAGTTTGTA 
CCATCACTTC CTTGTGAAAC TTCACAAGAA GGTATCAAGG AGCATGATGC AGATCCTAGT 
AAGAGATCAA CTCCTGCGCA CTCTTTATTC ACTGGAAACG ATTCTGTTCC TTGTCCTCCG 
GGTACTCAGA GAACAGAGAG GAAGCTCAGT TTACCCAAGA AGAAGACAAA GAAGCCTGTA 
ATCGATAATG GGAAGAGCAC TGTGATCAGT TTTAGTAACG GCATTGATGG AAGTCAAGTT 
AACTCGCATA CTGGTCCTTC CATGAACACA GTATCCCAAA CTCGAGACTT ATTGAATGGG 
AAAAGGGTGG GCGGTTTATT TGACAACCGT TTGGCTTCAG ATGGATATTT CAGAAAATAT 
CTCTCTCAGG TTAATGATAA GCCGATAACA TCTTTGCATT TGCAAGACAA TGATTATGTG 
AGGTCAAGAG ACGCGGAACC AAACTGTCTT CGAGATTTTA GTTCCTCTTC TAAATCCAGC 
TCAGGTGGAT GGTTGAGAAC TGGAGTAGAT ATTGTTGACT TCAGAAACAA CAACCACAAT 
ACAAACAGAT CGTCTTTCTC GAACTTGAAG CTAAGATACC CCCCTTCTTC TACTGAAGTT 
GCGGATTTAT CTCGGGTGCT GCAAAAGGAT GCTTCTGGTG CAGATAGAAA GGGGAAGACT 
GTTATGGTCC AAGAACATCA TGGAGCACCA AGAAGCCAAA GTCACGATAG AAAGGAGACT 
ACGACTGAAG AGCAAAACAA CGATGATATT CCAATGGAGA TAGTGGAGCT CATGGCCAAA 
AACCAGTACG AGAGGTGTCT TCCCGACAAA GAAGAAGATG TTAGCAACAA ACAGCCATCA 
CAAGAAACAG CACACAAATC CAAGAATGCT CTACTGATTG ATCTCAATGA AACCTACGAT 
AACGGGATCT CACTTGAGGA CAACAACACA TCAAGACCAC CAAAACCGTG TAGTAGCAAC 
GCAAGGAGGG AAGAACATTT TCCTATGGGA AGACAGCAGA ACTCTCATGA CTTCTTCCCA 
ATAAGTCAGC CTTATGTGCC TTCTCCGTTT GGGATCTTTC CTCCTACCCA AGAAAACCGA 
GCCAGCTCCA TCCGGTTTTC TGGTCACAAC TGTCAGTGGC TTGGGAATTT GCCAACTGTG 
GGTAATCAGA ACCCTTCTCC ATCCTCATTT CGGGTATTAC GTGCTTGTGA TACTTGCCAG 
AGTGTTCCTA ATCAATACAG AGAAGCTTCT CATCCAATTT GGCCATCTTC CATGATACCA 
CCACAGAGTC AATACAAGCC AGTTTCTTTA AATATTAATC AGTCAACAAA TCCGGGTACG 
CTTTCACAGG CATCTAACAA TGAAAATACA TGGAACCTCA ACTTTGTTGC TGCCAACGGG 
AAGCAAAAAT GTGGGCCTAA TCCAGAATTT TCATTTGGCT GCAAACATGC TGCTGGGGTT 
AGTAGTAGTA GTAGTAGGCC AATAGATAAC TTTTCTAGTG AGAGCTCTAT ACCGGCATTG 
CATCTACTCA GCCTTCTGGA TCCTCGCCTG AGGTCAACGA CTCCCGCTGA CCAACACGGA 
AACACTAAAT TTACTAAAAG ACATTTTCCG CCAGCCAACC AGTCCAAAGA GTTTATAGAG 
CTTCAAACAG GGGACTCTAG TAAGTCAGCC TACTCAACTA AGCAGATACC TTTTGATCTA 
TACAGCAAGA GATTCACACA AGAGCCTTCC CGGAAGAGTT TCCCCATCAC TCCACCTATT 
GGGACGTCTT CACTTTCATT TCAAAATGCT TCATGGAGTC CTCATCATCA AGAGAAGAAA 
ACCAAGAGAA AAGACACCTT TGCTCCTGTT TACAATACTC ATGAAAAGCC GGTGTTTGCA 
AGCAGCAACG ACCAAGCGAA GTTCCAGCTG CTGGGAGCAT CGAATTCCAT GATGCTTCCT 
TTGAAATTTC ACATGACGGA TAAAGAAAAG AAACAAAAGA GAAAAGCAGA GAGCTGCAAT 
AACAATGCCT CTGCGGGACC TGTGAAGAAC AGTTCTGGAC CCATTGTGTG CAGCGTCAAT 
AGAAACCCTG CTGATTTCAC CATTCCTGAA CCTGGGAATG TTTACATGTT AACGGGTGAA 
CATCTGAAAG TCCGTAAACG CACAACCTTC AAGAAAAAGC CAGCCGTGTG TAAGCAGGAC 
GCAATGAAGC AGACCAAGAA GCCGGTTTGT CCACCTACAC AAAATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196714.1
CDS	456..3746
Translation	MGSSIKINSISIDLAGAANEIDMVKCDHFSMRGFVAETRERDLRKCWPFSEESVSLVDQQSYTLPTLSVPKFRWWHCMSCIKDIDAHGPKDCGLHSNSKAIGNSSVIESKSKFNSLTIIDHEKEKKTDIADNAIEEKVGVNCENDDQTATTFLKKARGRPMGASNVRSKSRKLVSPEQVGNNRSKEKLNKPSMDISSWKEKQNVDQAVTTFGSSEIAGVVEDTPPKATKNHKGIRGLMECDNGSSESINLAMSGLQRRKSRKVRLLSELLGNTKTSGGSNIRKEESALKKESVRGRKRKLLPENNYVSRILSTMGATSENASKSCDSDQGNSESTDSGFDRTPFKGKQRNRRFQVVDEFVPSLPCETSQEGIKEHDADPSKRSTPAHSLFTGNDSVPCPPGTQRTERKLSLPKKKTKKPVIDNGKSTVISFSNGIDGSQVNSHTGPSMNTVSQTRDLLNGKRVGGLFDNRLASDGYFRKYLSQVNDKPITSLHLQDNDYVRSRDAEPNCLRDFSSSSKSSSGGWLRTGVDIVDFRNNNHNTNRSSFSNLKLRYPPSSTEVADLSRVLQKDASGADRKGKTVMVQEHHGAPRSQSHDRKETTTEEQNNDDIPMEIVELMAKNQYERCLPDKEEDVSNKQPSQETAHKSKNALLIDLNETYDNGISLEDNNTSRPPKPCSSNARREEHFPMGRQQNSHDFFPISQPYVPSPFGIFPPTQENRASSIRFSGHNCQWLGNLPTVGNQNPSPSSFRVLRACDTCQSVPNQYREASHPIWPSSMIPPQSQYKPVSLNINQSTNPGTLSQASNNENTWNLNFVAANGKQKCGPNPEFSFGCKHAAGVSSSSSRPIDNFSSESSIPALHLLSLLDPRLRSTTPADQHGNTKFTKRHFPPANQSKEFIELQTGDSSKSAYSTKQIPFDLYSKRFTQEPSRKSFPITPPIGTSSLSFQNASWSPHHQEKKTKRKDTFAPVYNTHEKPVFASSNDQAKFQLLGASNSMMLPLKFHMTDKEKKQKRKAESCNNNASAGPVKNSSGPIVCSVNRNPADFTIPEPGNVYMLTGEHLKVRKRTTFKKKPAVCKQDAMKQTKKPVCPPTQNA

Target ORF information:

RefSeq Version	NM_121191.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana embryonic flower 1 (EMF1) (EMF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_121191.4

ORF Insert Sequence:

ATGGGATCTT CCATCAAGAT CAACTCAATA TCCATAGATC TCGCAGGTGC TGCCAACGAG 
ATTGATATGG TGAAATGTGA TCATTTTTCC ATGCGTGGAT TCGTAGCTGA AACTCGTGAG 
AGAGACCTTA GAAAATGTTG GCCGTTTTCA GAAGAGAGTG TTAGTTTAGT AGACCAACAA 
AGCTATACTC TTCCTACTTT ATCTGTTCCA AAGTTTAGAT GGTGGCATTG TATGAGCTGC 
ATCAAAGATA TAGATGCTCA TGGGCCAAAA GATTGTGGAC TGCATTCAAA CTCAAAAGCT 
ATTGGAAACT CTTCTGTTAT CGAAAGTAAA AGCAAGTTCA ATTCGCTAAC TATCATTGAT 
CACGAGAAAG AAAAGAAAAC TGATATTGCA GATAATGCTA TTGAGGAGAA AGTGGGTGTA 
AACTGTGAGA ATGATGATCA GACAGCTACT ACGTTTCTCA AGAAAGCACG TGGTCGACCT 
ATGGGTGCTT CTAATGTTAG GAGCAAGAGC AGAAAGCTTG TGAGTCCGGA GCAGGTAGGA 
AACAACAGAT CTAAAGAAAA ACTAAACAAA CCATCGATGG ATATTAGCAG CTGGAAAGAG 
AAACAAAATG TGGATCAGGC TGTGACAACG TTCGGCTCAT CTGAAATTGC TGGTGTGGTT 
GAGGATACAC CACCTAAGGC AACCAAGAAT CATAAAGGCA TTCGCGGTCT GATGGAATGC 
GATAACGGGT CATCAGAAAG TATAAATCTT GCTATGAGTG GGTTGCAGCG TAGGAAATCT 
CGCAAGGTTC GTCTACTCAG TGAGTTGCTT GGTAATACAA AAACCAGTGG TGGTAGTAAC 
ATCAGAAAAG AAGAGTCTGC TTTGAAGAAG GAATCAGTTA GAGGTCGAAA AAGAAAGTTG 
TTACCTGAAA ACAATTATGT CAGCCGGATA TTGAGTACAA TGGGTGCAAC CTCTGAAAAT 
GCTTCCAAAA GTTGTGACTC TGATCAAGGT AATAGTGAAT CAACTGATAG TGGGTTTGAC 
AGAACTCCAT TTAAGGGTAA GCAGAGAAAC AGAAGATTTC AGGTTGTTGA CGAGTTTGTA 
CCATCACTTC CTTGTGAAAC TTCACAAGAA GGTATCAAGG AGCATGATGC AGATCCTAGT 
AAGAGATCAA CTCCTGCGCA CTCTTTATTC ACTGGAAACG ATTCTGTTCC TTGTCCTCCG 
GGTACTCAGA GAACAGAGAG GAAGCTCAGT TTACCCAAGA AGAAGACAAA GAAGCCTGTA 
ATCGATAATG GGAAGAGCAC TGTGATCAGT TTTAGTAACG GCATTGATGG AAGTCAAGTT 
AACTCGCATA CTGGTCCTTC CATGAACACA GTATCCCAAA CTCGAGACTT ATTGAATGGG 
AAAAGGGTGG GCGGTTTATT TGACAACCGT TTGGCTTCAG ATGGATATTT CAGAAAATAT 
CTCTCTCAGG TTAATGATAA GCCGATAACA TCTTTGCATT TGCAAGACAA TGATTATGTG 
AGGTCAAGAG ACGCGGAACC AAACTGTCTT CGAGATTTTA GTTCCTCTTC TAAATCCAGC 
TCAGGTGGAT GGTTGAGAAC TGGAGTAGAT ATTGTTGACT TCAGAAACAA CAACCACAAT 
ACAAACAGAT CGTCTTTCTC GAACTTGAAG CTAAGATACC CCCCTTCTTC TACTGAAGTT 
GCGGATTTAT CTCGGGTGCT GCAAAAGGAT GCTTCTGGTG CAGATAGAAA GGGGAAGACT 
GTTATGGTCC AAGAACATCA TGGAGCACCA AGAAGCCAAA GTCACGATAG AAAGGAGACT 
ACGACTGAAG AGCAAAACAA CGATGATATT CCAATGGAGA TAGTGGAGCT CATGGCCAAA 
AACCAGTACG AGAGGTGTCT TCCCGACAAA GAAGAAGATG TTAGCAACAA ACAGCCATCA 
CAAGAAACAG CACACAAATC CAAGAATGCT CTACTGATTG ATCTCAATGA AACCTACGAT 
AACGGGATCT CACTTGAGGA CAACAACACA TCAAGACCAC CAAAACCGTG TAGTAGCAAC 
GCAAGGAGGG AAGAACATTT TCCTATGGGA AGACAGCAGA ACTCTCATGA CTTCTTCCCA 
ATAAGTCAGC CTTATGTGCC TTCTCCGTTT GGGATCTTTC CTCCTACCCA AGAAAACCGA 
GCCAGCTCCA TCCGGTTTTC TGGTCACAAC TGTCAGTGGC TTGGGAATTT GCCAACTGTG 
GGTAATCAGA ACCCTTCTCC ATCCTCATTT CGGGTATTAC GTGCTTGTGA TACTTGCCAG 
AGTGTTCCTA ATCAATACAG AGAAGCTTCT CATCCAATTT GGCCATCTTC CATGATACCA 
CCACAGAGTC AATACAAGCC AGTTTCTTTA AATATTAATC AGTCAACAAA TCCGGGTACG 
CTTTCACAGG CATCTAACAA TGAAAATACA TGGAACCTCA ACTTTGTTGC TGCCAACGGG 
AAGCAAAAAT GTGGGCCTAA TCCAGAATTT TCATTTGGCT GCAAACATGC TGCTGGGGTT 
AGTAGTAGTA GTAGTAGGCC AATAGATAAC TTTTCTAGTG AGAGCTCTAT ACCGGCATTG 
CATCTACTCA GCCTTCTGGA TCCTCGCCTG AGGTCAACGA CTCCCGCTGA CCAACACGGA 
AACACTAAAT TTACTAAAAG ACATTTTCCG CCAGCCAACC AGTCCAAAGA GTTTATAGAG 
CTTCAAACAG GGGACTCTAG TAAGTCAGCC TACTCAACTA AGCAGATACC TTTTGATCTA 
TACAGCAAGA GATTCACACA AGAGCCTTCC CGGAAGAGTT TCCCCATCAC TCCACCTATT 
GGGACGTCTT CACTTTCATT TCAAAATGCT TCATGGAGTC CTCATCATCA AGAGAAGAAA 
ACCAAGAGAA AAGACACCTT TGCTCCTGTT TACAATACTC ATGAAAAGCC GGTGTTTGCA 
AGCAGCAACG ACCAAGCGAA GTTCCAGCTG CTGGGAGCAT CGAATTCCAT GATGCTTCCT 
TTGAAATTTC ACATGACGGA TAAAGAAAAG AAACAAAAGA GAAAAGCAGA GAGCTGCAAT 
AACAATGCCT CTGCGGGACC TGTGAAGAAC AGTTCTGGAC CCATTGTGTG CAGCGTCAAT 
AGAAACCCTG CTGATTTCAC CATTCCTGAA CCTGGGAATG TTTACATGTT AACGGGTGAA 
CATCTGAAAG TCCGTAAACG CACAACCTTC AAGAAAAAGC CAGCCGTGTG TAAGCAGGAC 
GCAATGAAGC AGACCAAGAA GCCGGTTTGT CCACCTACAC AAAATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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EMF1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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