EMB2771 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMB2771
Entrez Gene ID	830439
Full Name	E3 ubiquitin ligase
Synonyms	APC1,EMBRYO DEFECTIVE 2771,MOP10.10,MOP10_10,anaphase promoting complex 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001342825.1	NP_001331419.1	E3 ubiquitin ligase
NM_001342826.1	NP_001331420.1	E3 ubiquitin ligase
NM_001342824.1	NP_001318485.1	E3 ubiquitin ligase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANAPC1	XP_005630492.1
Rattus norvegicus (Norway rat)	Anapc1	NP_001101241.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000374	XP_310737.5
Arabidopsis thaliana (thale cress)	EMB2771	NP_196175.1
Oryza sativa (rice)	Os05g0354300	NP_001055285.1
Drosophila melanogaster (fruit fly)	shtd	NP_573025.2
Xenopus tropicalis (tropical clawed frog)	anapc1	NP_001072866.1
Homo sapiens (human)	ANAPC1	NP_073153.1
Mus musculus (house mouse)	Anapc1	NP_032595.2
Danio rerio (zebrafish)	anapc1	XP_001921168.2
Bos taurus (cattle)	ANAPC1	XP_005212426.1
Gallus gallus (chicken)	ANAPC1	XP_419303.3

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EMB2771 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMB2771 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb40306	NM_001342824.1 Latest version!	Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAb40585	NM_001342826.1 Latest version!	Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAb40586	NM_001342825.1 Latest version!	Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMB2771 ( NM_001342824.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001318485.1 Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.

Price & Availability↑

CloneID	OAb40306
Clone ID Related Accession (Same CDS sequence)	NM_001342824.1
Accession Version	NM_001342824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	E3 ubiquitin ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGCCTCCAG GGGTTCGCCA ACTTACCGTC CTCGGAAAGT TTAAACCTTT CGGTCTAATC 
GCCGAAGCTA CTGATGGTAA ATCACCGGAT GATTCCTATC AGTATTTCCT CTTCGATCCT 
GAGCTCACTG GCGAGCGAGA TGATGCTGAT GGCAATGATG CCAACTTCTC GCGGCAGAGA 
GAGCACGAGC TCTTCATCCG AGACAACTGC ATAATCTGGA CAAGCGGTTC GCGGGTCCTC 
AAGCGTTTTA CCTTGTCTTC TGCCGTCATT AAGGCATGCT GGAGCAACCT TGGTCGAGGG 
ACAGAAGCTT TTCTTTGTGT ATTACAAATT GCTTGCTTAA CAATCTACAA CACATCAGGC 
GAAGTTGTAT CTGTTCCACT AATGCGTACT GTCAAATCAA TATGGCCTCT ACCATGTGGT 
CTACTTCTTG AGCAAGCTGG TGAAGTGAAT CCACCCTCAC ATGTTCCATT TTCTCCTGTT 
AGCCCCATCC TTGGTTCTCG TGAGATGCTG CGCCAAAGAA AAGAAGTTGG GAATAGTTCA 
CCACAAAACT TCCATTCCCC AGTCGCGCAT GACCTTATTA GCAAAAGAGA TATGCCATGC 
ATGTCTTCCC ATCTGATTCT AAGAGATCCA CTAGAGGAAC CTGGGCCAAC CTACGTTGAA 
GAGAGGGGGA AGTTGACCAT AATGAAGGAC TACGATGAGA GGACAATTTG GACAAGTGAC 
CGTCTACCTC TTATGACGTC GTATAACAAA GGCAAGATGC AACATTCTGT GTGGGCGGCC 
GAATTTATAG AGTCAAACCT TGAAGCTTCT GCGTCATGTT CAAGCGGCAT TGTTCCTGAT 
GCTGTTCTCT CGAAGCGGGT TTCTTTTCGT AGAATATGGC AAGCGAAAGG TGCTAAGAAA 
GCTGCATCTA AGGTCTTTTT GGCAACTGAT AATTCAGTCC CAGTAATTTG CTTTCTGATT 
CTAGAACAAA AGAAGCTGTT ATCGGTGGGA CTTCAAACTG TTGAAATCAA TAACGAAATT 
CTGTTCGATG TTAAGCCTGA TATAAGCTGG AGTGTGTCTG CGATTGCTGC TGCACCTGTT 
GTTGTGACAC GTTCTCAAGT GAAAATAGGA CTGCTTCCAC ATTTGGATAT CATTGTTTTA 
TCTCCAGAAA ATGACCTCTT TCTCTATTCA GGAAAACAAT GTCTCTGTAG ATATGTATTA 
CCTTCTTGGT TGGGAGAAAG TATTGGTTCC GGGGATGGAG AGTCTGCAAA AACAGATTCA 
GGTTTCAGGA ACCTGAAGAT CACAGGATTG TCTGATGCTG TTTTAGGATC TATCAATCTC 
TCAGTTAACC ATTCACAGAT CTTTCGGTGT GCCTTAACTG GTAAACCTTC ATCGTCACTT 
GCAAATGATT GCATAGCAGC CATAGCTGAG GGACTACGAT CCGATCTGTA CAGTTTGTTT 
CTCTCTCTTC TTTGGGGAGA TGGTCATTCT GACCTGCAAG GTTCCAGTAT TCATTTTGAA 
TGGGAAGCAT TATGCAACAT TTTTCTGGAG ATCTGTCAAA AGCCTACTGT TGTGCATCGA 
AAGCAACCCA AAACAGCATC AGAGTCCTCC TGGGAGTTTC TTCTCATCAG CAAATTTCAT 
AAGACCTACT CTAGGTTTCA CAATGGTATT ACTTCAATAA ATCGTTTAGA TCTGGAAGGC 
ATTGTCCCAT TTGATTCCAA GATTTGTAGT GAAGAAACAC TGGGCAGCTC ATGTGAACTA 
ATGGTCCAGA GTTTAGATTG TCTCCATGCA GTTTATGAGA GCTTGAAGAT GGATAATCTA 
CGGAAACAGG ATTTGCATCA CTTGGCTGTT TTGTTGTGCA ATATTGCCAA GTTTCTGGAT 
GAGAAGTGTT ACTTAGATTA CTACATACGT GATTTTCCTC GTCTGTCAAC AACTATTGGG 
GCGTGCACAA CCCTTTCTTC TAGCAGAAAA CCTCCAAATT TATTTAGATG GCTTGAGAAT 
TGTTTAAGAC GTGGATGTTT ATCAACAAAC TTTGATGACC TCCCAGATTT GATCCGTAGA 
GATGGGTGCT CCATAGTAAG CTGGGCAAGG AAAGTTGTTT CCTTCTACAG TGTGTTATTT 
GGTGATAAGC CAGAAGGACG GACACTTTCA TCAGGTGTCC CCTGTAATAT CGCGCCAGGA 
TCATACTCCT GTAACGAGGA ACTCACTATC TTAGCTATGG CAGGAGAGAG ATTTGGGCTT 
CACCAACTGG ATTTGCTACC TTCTGGTGTT TCTCTCCCGC TAAGACATGC ACTGGATAGC 
TGTCGGGAAT CTCCTCCAGC TGACTGGCCA GCAATTGCTT ATGTGCTTCT TGGTCGAGAA 
GATATGGCCC TATCCGTCTT CAGAAATTTT AGTTCATCTA AGGAATTTGA GATGCAGTCA 
AATACGAGTT TGATATCGAT GTCCATACCT TACATGCTAC ATTTGCATCC AGTAATTGTT 
CCATCCTCTG AGTCGATTGG CTTAGAAAAC ACTAAGATTG AGGATACAAA TTCTGTGGAT 
GGTTCTGTGA TAGATGGAAT GGAGCATATC TTCAACTCCT ACACACAGCT ACGGTATGGC 
CGGGATCTAA GATTGAATGA GGTTAGACGT CTTTTGTGCT CTGCAAGACC TGTGGTAGTT 
CAAACGGCTG CTAACCCTAC TATATCAGAT CAGGAGCAGC AACAGGACCA TCTTTGGCGC 
ATAGCACAGA GGACTGCTGT TCTACCCCTC GGGCGTGGAG CATTCACATT ATCAACGATA 
CATACTCTTT TAACTGAGGC GTTTACTGTA CCAAAGCTTG TTTTAGCTGG TCGACTGCCT 
TCTCAACAAA ATGCCATTGT TAATCTAGAT CCAAACATAA GGAATATCCA AGAATTAAAG 
ACCTGGCCAG AGTTTCATAA CGCTGTTGCT GCTGGGTTAC GACTGGCTCC GCTTCAGGGA 
AAGGTCTCCA GAACGTGGAT TAGATACAAT AAACCTGGAG AGCCAAATGC TGTTCATGCT 
GGACTTCTGT TTGGCCTGGG ATTACAGGGA TATTTGCATG TGTTAAACCT TAGTGACATA 
TACCAATATT TCACTCAGGA CCATGAGAGC ACTACGGTAG GTTTGATGCT TGGTCTGGCC 
GCTTCATACA GGGGAACAAT GCAACCTGAT ATTGCAAAGG CTCTCTTTTT CCATGTTCCT 
GCTCGATACC AAGCTTCATA TACTGAGTTT GAGATACCAA CACTTCTACA GTCTGCAGCT 
TTAGTCTCCG TTGGCATGCT ATTTGAAGGA TCAGCACACC AGCAGACAAT GCAATTACTT 
TTGGGTGAAA TTGGCCGTAG AAGTGCAGGG GACAATGTCC TTGAAAGGGA GGGTTATGCT 
GTATCTGCAG GATTCTCACT TGGTCTTGTA GCTTTGGGCC GTGGAGGTGA TGCGCTCGGT 
TCCATGGACT CCTTGGTCAA TCGCTTACTC CTGTATTTAG GAGCAAAAGA GGAAAGATCT 
ATCCTTGTAC CATCCCTTGA AGATCACCGA AGTGCTGCAC AGATAACAGA CGGAAGCACT 
TCTAATGTTG ATATAACTGC ACCTGGAGCG ATAATAGCTC TCACATTAAT GTATCTTAAG 
ACAGAATCAG AGGTCATCTT CTCAAAGCTC TCTATTCCTC AAACACATTA CGATTTGGAG 
TGCGTTAGGC CTGATTTCAT TATGCTCCGT GTCATTGCTC GGAACTTGAT AATGTGGAGC 
AGGATCTGTC CTACTTGTGA TTGGATTCAG TCTCAAGTTC CTGAAGTAGT CAAAAATGGT 
ATCAGCCAAC TCCGAGATGA CATGGATAAT ATGTATGAGG TGGATGTTGA AGCCCTTGTT 
CAGGCATACG TCAATATAGT GGCCGGGGCC TGCATTTCAC TTGGGCTGAG ATTTGCTGGT 
ACGAGGGATG GAAATGCTCG TGACTTACTT AACTCCTACG CTCTCTATCT TTTGAATGAG 
ATCAAGCCTC TTTCTGCCAC ACCTGGAAAT GCATTTCCCA GGGGAATATC AAAATTTGTT 
GATCGGGGAA CACTTGAAAT GTGTCTTTAT CTTATCATTA TTTCTCTGTC AGTGGTCATG 
GCGGGATCTG GAGACTTGCA AGTTTTTCGG TTGTTGAGAT TTCTCCGCAG CCGAAACTCT 
GCCGATGGAC ATGCTAACTA TGGCACACAA ATGGCAGTGA GCTTAGCCAC AGGATTCTTG 
TTTCTTGGAG GCGGTATGCG AACATTTTCA ACGAATAATG GCTCACTGGC AATGTTGCTC 
ATTACTCTCT ATCCACGGTT GCCTTCTGGA CCAAATGACA ACCGTTGTCA CCTCCAGGCA 
TTCAGGCATT TATATGTCCT TGCAACAGAG GCTCGCTGGC TGCAGACCAT TGATGTTGAT 
TCTGGTCTGC CTGTCTATGC CCCTCTAGAA GTCACAGTAA AAGAGACAAA ACTTTATTCA 
GAAACAAAAT TTTGCGAGAT AACACCGTGC ATTCTGCCAG AGCGTGCTAT TCTAAAGAGA 
ATTTGTGTAT GTGGTCCCCG ATACTGGCCC CAACAAATTG AGCTAGTTTT TGGTTTAAGG 
ACTCTGGGAG AATCTAATTT GATTGCTAAT AGTCATAGAG AACTGGATTC TGATAGTGTT 
GATCACTTGG TCAGCACTTT CTCATCAGAT CCAAGCCTAA TAGCGTTTGC ACAGTTGTGC 
TGTGACAAAT CTTGGAATAA CAGCTTTGAT TTCCTAATTT TGGATCTAAT CTTATGGTCT 
CAGGTTGCTC TTGCCTACAA TGAGGCAGTA TCAACTGGAA GATTGGCCTC CTCAGGTGGC 
TTTGTACAGT CAATCTTCTT AGCATCGCTG AGAAAGCGAT GTGAAGAGGT TTTGAATTGC 
TCAACGGAGC TGAAGATTAA TTTGAGGAAT TATTTGACCT CAGAAGCATG GCCTTATGAT 
AAGAACTCAA AGTTGCAGAA AGATATAATT ATTCTCTCAT GGTATCTAAA ATGGTTCAAC 
GTACCATCTC CATCGATCAT CAAAGCAGCA GTGGAAAAGA TCAAATCCAA GTCCAAAAAC 
TCAACTTCGG CAATACCTTT GTTGCGATTG TTGTTACCAA ATACGCATAT TAGTGTGATT 
GGTGAAATTG ATAGGGTCTT TTTCCCCTCG AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001318485.1
CDS	88..5223
Translation	MPPGVRQLTVLGKFKPFGLIAEATDGKSPDDSYQYFLFDPELTGERDDADGNDANFSRQREHELFIRDNCIIWTSGSRVLKRFTLSSAVIKACWSNLGRGTEAFLCVLQIACLTIYNTSGEVVSVPLMRTVKSIWPLPCGLLLEQAGEVNPPSHVPFSPVSPILGSREMLRQRKEVGNSSPQNFHSPVAHDLISKRDMPCMSSHLILRDPLEEPGPTYVEERGKLTIMKDYDERTIWTSDRLPLMTSYNKGKMQHSVWAAEFIESNLEASASCSSGIVPDAVLSKRVSFRRIWQAKGAKKAASKVFLATDNSVPVICFLILEQKKLLSVGLQTVEINNEILFDVKPDISWSVSAIAAAPVVVTRSQVKIGLLPHLDIIVLSPENDLFLYSGKQCLCRYVLPSWLGESIGSGDGESAKTDSGFRNLKITGLSDAVLGSINLSVNHSQIFRCALTGKPSSSLANDCIAAIAEGLRSDLYSLFLSLLWGDGHSDLQGSSIHFEWEALCNIFLEICQKPTVVHRKQPKTASESSWEFLLISKFHKTYSRFHNGITSINRLDLEGIVPFDSKICSEETLGSSCELMVQSLDCLHAVYESLKMDNLRKQDLHHLAVLLCNIAKFLDEKCYLDYYIRDFPRLSTTIGACTTLSSSRKPPNLFRWLENCLRRGCLSTNFDDLPDLIRRDGCSIVSWARKVVSFYSVLFGDKPEGRTLSSGVPCNIAPGSYSCNEELTILAMAGERFGLHQLDLLPSGVSLPLRHALDSCRESPPADWPAIAYVLLGREDMALSVFRNFSSSKEFEMQSNTSLISMSIPYMLHLHPVIVPSSESIGLENTKIEDTNSVDGSVIDGMEHIFNSYTQLRYGRDLRLNEVRRLLCSARPVVVQTAANPTISDQEQQQDHLWRIAQRTAVLPLGRGAFTLSTIHTLLTEAFTVPKLVLAGRLPSQQNAIVNLDPNIRNIQELKTWPEFHNAVAAGLRLAPLQGKVSRTWIRYNKPGEPNAVHAGLLFGLGLQGYLHVLNLSDIYQYFTQDHESTTVGLMLGLAASYRGTMQPDIAKALFFHVPARYQASYTEFEIPTLLQSAALVSVGMLFEGSAHQQTMQLLLGEIGRRSAGDNVLEREGYAVSAGFSLGLVALGRGGDALGSMDSLVNRLLLYLGAKEERSILVPSLEDHRSAAQITDGSTSNVDITAPGAIIALTLMYLKTESEVIFSKLSIPQTHYDLECVRPDFIMLRVIARNLIMWSRICPTCDWIQSQVPEVVKNGISQLRDDMDNMYEVDVEALVQAYVNIVAGACISLGLRFAGTRDGNARDLLNSYALYLLNEIKPLSATPGNAFPRGISKFVDRGTLEMCLYLIIISLSVVMAGSGDLQVFRLLRFLRSRNSADGHANYGTQMAVSLATGFLFLGGGMRTFSTNNGSLAMLLITLYPRLPSGPNDNRCHLQAFRHLYVLATEARWLQTIDVDSGLPVYAPLEVTVKETKLYSETKFCEITPCILPERAILKRICVCGPRYWPQQIELVFGLRTLGESNLIANSHRELDSDSVDHLVSTFSSDPSLIAFAQLCCDKSWNNSFDFLILDLILWSQVALAYNEAVSTGRLASSGGFVQSIFLASLRKRCEEVLNCSTELKINLRNYLTSEAWPYDKNSKLQKDIIILSWYLKWFNVPSPSIIKAAVEKIKSKSKNSTSAIPLLRLLLPNTHISVIGEIDRVFFPSN

Target ORF information:

RefSeq Version	NM_001342824.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342824.1

ORF Insert Sequence:

ATGCCTCCAG GGGTTCGCCA ACTTACCGTC CTCGGAAAGT TTAAACCTTT CGGTCTAATC 
GCCGAAGCTA CTGATGGTAA ATCACCGGAT GATTCCTATC AGTATTTCCT CTTCGATCCT 
GAGCTCACTG GCGAGCGAGA TGATGCTGAT GGCAATGATG CCAACTTCTC GCGGCAGAGA 
GAGCACGAGC TCTTCATCCG AGACAACTGC ATAATCTGGA CAAGCGGTTC GCGGGTCCTC 
AAGCGTTTTA CCTTGTCTTC TGCCGTCATT AAGGCATGCT GGAGCAACCT TGGTCGAGGG 
ACAGAAGCTT TTCTTTGTGT ATTACAAATT GCTTGCTTAA CAATCTACAA CACATCAGGC 
GAAGTTGTAT CTGTTCCACT AATGCGTACT GTCAAATCAA TATGGCCTCT ACCATGTGGT 
CTACTTCTTG AGCAAGCTGG TGAAGTGAAT CCACCCTCAC ATGTTCCATT TTCTCCTGTT 
AGCCCCATCC TTGGTTCTCG TGAGATGCTG CGCCAAAGAA AAGAAGTTGG GAATAGTTCA 
CCACAAAACT TCCATTCCCC AGTCGCGCAT GACCTTATTA GCAAAAGAGA TATGCCATGC 
ATGTCTTCCC ATCTGATTCT AAGAGATCCA CTAGAGGAAC CTGGGCCAAC CTACGTTGAA 
GAGAGGGGGA AGTTGACCAT AATGAAGGAC TACGATGAGA GGACAATTTG GACAAGTGAC 
CGTCTACCTC TTATGACGTC GTATAACAAA GGCAAGATGC AACATTCTGT GTGGGCGGCC 
GAATTTATAG AGTCAAACCT TGAAGCTTCT GCGTCATGTT CAAGCGGCAT TGTTCCTGAT 
GCTGTTCTCT CGAAGCGGGT TTCTTTTCGT AGAATATGGC AAGCGAAAGG TGCTAAGAAA 
GCTGCATCTA AGGTCTTTTT GGCAACTGAT AATTCAGTCC CAGTAATTTG CTTTCTGATT 
CTAGAACAAA AGAAGCTGTT ATCGGTGGGA CTTCAAACTG TTGAAATCAA TAACGAAATT 
CTGTTCGATG TTAAGCCTGA TATAAGCTGG AGTGTGTCTG CGATTGCTGC TGCACCTGTT 
GTTGTGACAC GTTCTCAAGT GAAAATAGGA CTGCTTCCAC ATTTGGATAT CATTGTTTTA 
TCTCCAGAAA ATGACCTCTT TCTCTATTCA GGAAAACAAT GTCTCTGTAG ATATGTATTA 
CCTTCTTGGT TGGGAGAAAG TATTGGTTCC GGGGATGGAG AGTCTGCAAA AACAGATTCA 
GGTTTCAGGA ACCTGAAGAT CACAGGATTG TCTGATGCTG TTTTAGGATC TATCAATCTC 
TCAGTTAACC ATTCACAGAT CTTTCGGTGT GCCTTAACTG GTAAACCTTC ATCGTCACTT 
GCAAATGATT GCATAGCAGC CATAGCTGAG GGACTACGAT CCGATCTGTA CAGTTTGTTT 
CTCTCTCTTC TTTGGGGAGA TGGTCATTCT GACCTGCAAG GTTCCAGTAT TCATTTTGAA 
TGGGAAGCAT TATGCAACAT TTTTCTGGAG ATCTGTCAAA AGCCTACTGT TGTGCATCGA 
AAGCAACCCA AAACAGCATC AGAGTCCTCC TGGGAGTTTC TTCTCATCAG CAAATTTCAT 
AAGACCTACT CTAGGTTTCA CAATGGTATT ACTTCAATAA ATCGTTTAGA TCTGGAAGGC 
ATTGTCCCAT TTGATTCCAA GATTTGTAGT GAAGAAACAC TGGGCAGCTC ATGTGAACTA 
ATGGTCCAGA GTTTAGATTG TCTCCATGCA GTTTATGAGA GCTTGAAGAT GGATAATCTA 
CGGAAACAGG ATTTGCATCA CTTGGCTGTT TTGTTGTGCA ATATTGCCAA GTTTCTGGAT 
GAGAAGTGTT ACTTAGATTA CTACATACGT GATTTTCCTC GTCTGTCAAC AACTATTGGG 
GCGTGCACAA CCCTTTCTTC TAGCAGAAAA CCTCCAAATT TATTTAGATG GCTTGAGAAT 
TGTTTAAGAC GTGGATGTTT ATCAACAAAC TTTGATGACC TCCCAGATTT GATCCGTAGA 
GATGGGTGCT CCATAGTAAG CTGGGCAAGG AAAGTTGTTT CCTTCTACAG TGTGTTATTT 
GGTGATAAGC CAGAAGGACG GACACTTTCA TCAGGTGTCC CCTGTAATAT CGCGCCAGGA 
TCATACTCCT GTAACGAGGA ACTCACTATC TTAGCTATGG CAGGAGAGAG ATTTGGGCTT 
CACCAACTGG ATTTGCTACC TTCTGGTGTT TCTCTCCCGC TAAGACATGC ACTGGATAGC 
TGTCGGGAAT CTCCTCCAGC TGACTGGCCA GCAATTGCTT ATGTGCTTCT TGGTCGAGAA 
GATATGGCCC TATCCGTCTT CAGAAATTTT AGTTCATCTA AGGAATTTGA GATGCAGTCA 
AATACGAGTT TGATATCGAT GTCCATACCT TACATGCTAC ATTTGCATCC AGTAATTGTT 
CCATCCTCTG AGTCGATTGG CTTAGAAAAC ACTAAGATTG AGGATACAAA TTCTGTGGAT 
GGTTCTGTGA TAGATGGAAT GGAGCATATC TTCAACTCCT ACACACAGCT ACGGTATGGC 
CGGGATCTAA GATTGAATGA GGTTAGACGT CTTTTGTGCT CTGCAAGACC TGTGGTAGTT 
CAAACGGCTG CTAACCCTAC TATATCAGAT CAGGAGCAGC AACAGGACCA TCTTTGGCGC 
ATAGCACAGA GGACTGCTGT TCTACCCCTC GGGCGTGGAG CATTCACATT ATCAACGATA 
CATACTCTTT TAACTGAGGC GTTTACTGTA CCAAAGCTTG TTTTAGCTGG TCGACTGCCT 
TCTCAACAAA ATGCCATTGT TAATCTAGAT CCAAACATAA GGAATATCCA AGAATTAAAG 
ACCTGGCCAG AGTTTCATAA CGCTGTTGCT GCTGGGTTAC GACTGGCTCC GCTTCAGGGA 
AAGGTCTCCA GAACGTGGAT TAGATACAAT AAACCTGGAG AGCCAAATGC TGTTCATGCT 
GGACTTCTGT TTGGCCTGGG ATTACAGGGA TATTTGCATG TGTTAAACCT TAGTGACATA 
TACCAATATT TCACTCAGGA CCATGAGAGC ACTACGGTAG GTTTGATGCT TGGTCTGGCC 
GCTTCATACA GGGGAACAAT GCAACCTGAT ATTGCAAAGG CTCTCTTTTT CCATGTTCCT 
GCTCGATACC AAGCTTCATA TACTGAGTTT GAGATACCAA CACTTCTACA GTCTGCAGCT 
TTAGTCTCCG TTGGCATGCT ATTTGAAGGA TCAGCACACC AGCAGACAAT GCAATTACTT 
TTGGGTGAAA TTGGCCGTAG AAGTGCAGGG GACAATGTCC TTGAAAGGGA GGGTTATGCT 
GTATCTGCAG GATTCTCACT TGGTCTTGTA GCTTTGGGCC GTGGAGGTGA TGCGCTCGGT 
TCCATGGACT CCTTGGTCAA TCGCTTACTC CTGTATTTAG GAGCAAAAGA GGAAAGATCT 
ATCCTTGTAC CATCCCTTGA AGATCACCGA AGTGCTGCAC AGATAACAGA CGGAAGCACT 
TCTAATGTTG ATATAACTGC ACCTGGAGCG ATAATAGCTC TCACATTAAT GTATCTTAAG 
ACAGAATCAG AGGTCATCTT CTCAAAGCTC TCTATTCCTC AAACACATTA CGATTTGGAG 
TGCGTTAGGC CTGATTTCAT TATGCTCCGT GTCATTGCTC GGAACTTGAT AATGTGGAGC 
AGGATCTGTC CTACTTGTGA TTGGATTCAG TCTCAAGTTC CTGAAGTAGT CAAAAATGGT 
ATCAGCCAAC TCCGAGATGA CATGGATAAT ATGTATGAGG TGGATGTTGA AGCCCTTGTT 
CAGGCATACG TCAATATAGT GGCCGGGGCC TGCATTTCAC TTGGGCTGAG ATTTGCTGGT 
ACGAGGGATG GAAATGCTCG TGACTTACTT AACTCCTACG CTCTCTATCT TTTGAATGAG 
ATCAAGCCTC TTTCTGCCAC ACCTGGAAAT GCATTTCCCA GGGGAATATC AAAATTTGTT 
GATCGGGGAA CACTTGAAAT GTGTCTTTAT CTTATCATTA TTTCTCTGTC AGTGGTCATG 
GCGGGATCTG GAGACTTGCA AGTTTTTCGG TTGTTGAGAT TTCTCCGCAG CCGAAACTCT 
GCCGATGGAC ATGCTAACTA TGGCACACAA ATGGCAGTGA GCTTAGCCAC AGGATTCTTG 
TTTCTTGGAG GCGGTATGCG AACATTTTCA ACGAATAATG GCTCACTGGC AATGTTGCTC 
ATTACTCTCT ATCCACGGTT GCCTTCTGGA CCAAATGACA ACCGTTGTCA CCTCCAGGCA 
TTCAGGCATT TATATGTCCT TGCAACAGAG GCTCGCTGGC TGCAGACCAT TGATGTTGAT 
TCTGGTCTGC CTGTCTATGC CCCTCTAGAA GTCACAGTAA AAGAGACAAA ACTTTATTCA 
GAAACAAAAT TTTGCGAGAT AACACCGTGC ATTCTGCCAG AGCGTGCTAT TCTAAAGAGA 
ATTTGTGTAT GTGGTCCCCG ATACTGGCCC CAACAAATTG AGCTAGTTTT TGGTTTAAGG 
ACTCTGGGAG AATCTAATTT GATTGCTAAT AGTCATAGAG AACTGGATTC TGATAGTGTT 
GATCACTTGG TCAGCACTTT CTCATCAGAT CCAAGCCTAA TAGCGTTTGC ACAGTTGTGC 
TGTGACAAAT CTTGGAATAA CAGCTTTGAT TTCCTAATTT TGGATCTAAT CTTATGGTCT 
CAGGTTGCTC TTGCCTACAA TGAGGCAGTA TCAACTGGAA GATTGGCCTC CTCAGGTGGC 
TTTGTACAGT CAATCTTCTT AGCATCGCTG AGAAAGCGAT GTGAAGAGGT TTTGAATTGC 
TCAACGGAGC TGAAGATTAA TTTGAGGAAT TATTTGACCT CAGAAGCATG GCCTTATGAT 
AAGAACTCAA AGTTGCAGAA AGATATAATT ATTCTCTCAT GGTATCTAAA ATGGTTCAAC 
GTACCATCTC CATCGATCAT CAAAGCAGCA GTGGAAAAGA TCAAATCCAA GTCCAAAAAC 
TCAACTTCGG CAATACCTTT GTTGCGATTG TTGTTACCAA ATACGCATAT TAGTGTGATT 
GGTGAAATTG ATAGGGTCTT TTTCCCCTCG AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMB2771 ( NM_001342826.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331420.1 Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.

Price & Availability↑

CloneID	OAb40585
Clone ID Related Accession (Same CDS sequence)	NM_001342826.1
Accession Version	NM_001342826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	E3 ubiquitin ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGCGTACTG TCAAATCAAT ATGGCCTCTA CCATGTGGTC TACTTCTTGA GCAAGCTGGT 
GAAGTGAATC CACCCTCACA TGTTCCATTT TCTCCTGTTA GCCCCATCCT TGGTTCTCGT 
GAGATGCTGC GCCAAAGAAA AGAAGTTGGG AATAGTTCAC CACAAAACTT CCATTCCCCA 
GTCGCGCATG ACCTTATTAG CAAAAGAGAT ATGCCATGCA TGTCTTCCCA TCTGATTCTA 
AGAGATCCAC TAGAGGAACC TGGGCCAACC TACGTTGAAG AGAGGGGGAA GTTGACCATA 
ATGAAGGACT ACGATGAGAG GACAATTTGG ACAAGTGACC GTCTACCTCT TATGACGTCG 
TATAACAAAG GCAAGATGCA ACATTCTGTG TGGGCGGCCG AATTTATAGA GTCAAACCTT 
GAAGCTTCTG CGTCATGTTC AAGCGGCATT GTTCCTGATG CTGTTCTCTC GAAGCGGGTT 
TCTTTTCGTA GAATATGGCA AGCGAAAGGT GCTAAGAAAG CTGCATCTAA GGTCTTTTTG 
GCAACTGATA ATTCAGTCCC AGTAATTTGC TTTCTGATTC TAGAACAAAA GAAGCTGTTA 
TCGGTGGGAC TTCAAACTGT TGAAATCAAT AACGAAATTC TGTTCGATGT TAAGCCTGAT 
ATAAGCTGGA GTGTGTCTGC GATTGCTGCT GCACCTGTTG TTGTGACACG TTCTCAAGTG 
AAAATAGGAC TGCTTCCACA TTTGGATATC ATTGTTTTAT CTCCAGAAAA TGACCTCTTT 
CTCTATTCAG GAAAACAATG TCTCTGTAGA TATGTATTAC CTTCTTGGTT GGGAGAAAGT 
ATTGGTTCCG GGGATGGAGA GTCTGCAAAA ACAGATTCAG GTTTCAGGAA CCTGAAGATC 
ACAGGATTGT CTGATGCTGT TTTAGGATCT ATCAATCTCT CAGTTAACCA TTCACAGATC 
TTTCGGTGTG CCTTAACTGG TAAACCTTCA TCGTCACTTG CAAATGATTG CATAGCAGCC 
ATAGCTGAGG GACTACGATC CGATCTGTAC AGTTTGTTTC TCTCTCTTCT TTGGGGAGAT 
GGTCATTCTG ACCTGCAAGG TTCCAGTATT CATTTTGAAT GGGAAGCATT ATGCAACATT 
TTTCTGGAGA TCTGTCAAAA GCCTACTGTT GTGCATCGAA AGCAACCCAA AACAGCATCA 
GAGTCCTCCT GGGAGTTTCT TCTCATCAGC AAATTTCATA AGACCTACTC TAGGTTTCAC 
AATGGTATTA CTTCAATAAA TCGTTTAGAT CTGGAAGGCA TTGTCCCATT TGATTCCAAG 
ATTTGTAGTG AAGAAACACT GGGCAGCTCA TGTGAACTAA TGGTCCAGAG TTTAGATTGT 
CTCCATGCAG TTTATGAGAG CTTGAAGATG GATAATCTAC GGAAACAGGA TTTGCATCAC 
TTGGCTGTTT TGTTGTGCAA TATTGCCAAG TTTCTGGATG AGAAGTGTTA CTTAGATTAC 
TACATACGTG ATTTTCCTCG TCTGTCAACA ACTATTGGGG CGTGCACAAC CCTTTCTTCT 
AGCAGAAAAC CTCCAAATTT ATTTAGATGG CTTGAGAATT GTTTAAGACG TGGATGTTTA 
TCAACAAACT TTGATGACCT CCCAGATTTG ATCCGTAGAG ATGGGTGCTC CATAGTAAGC 
TGGGCAAGGA AAGTTGTTTC CTTCTACAGT GTGTTATTTG GTGATAAGCC AGAAGGACGG 
ACACTTTCAT CAGGTGTCCC CTGTAATATC GCGCCAGGAT CATACTCCTG TAACGAGGAA 
CTCACTATCT TAGCTATGGC AGGAGAGAGA TTTGGGCTTC ACCAACTGGA TTTGCTACCT 
TCTGGTGTTT CTCTCCCGCT AAGACATGCA CTGGATAGCT GTCGGGAATC TCCTCCAGCT 
GACTGGCCAG CAATTGCTTA TGTGCTTCTT GGTCGAGAAG ATATGGCCCT ATCCGTCTTC 
AGAAATTTTA GTTCATCTAA GGAATTTGAG ATGCAGTCAA ATACGAGTTT GATATCGATG 
TCCATACCTT ACATGCTACA TTTGCATCCA GTAATTGTTC CATCCTCTGA GTCGATTGGC 
TTAGAAAACA CTAAGATTGA GGATACAAAT TCTGTGGATG GTTCTGTGAT AGATGGAATG 
GAGCATATCT TCAACTCCTA CACACAGCTA CGGTATGGCC GGGATCTAAG ATTGAATGAG 
GTTAGACGTC TTTTGTGCTC TGCAAGACCT GTGGTAGTTC AAACGGCTGC TAACCCTACT 
ATATCAGATC AGGAGCAGCA ACAGGACCAT CTTTGGCGCA TAGCACAGAG GACTGCTGTT 
CTACCCCTCG GGCGTGGAGC ATTCACATTA TCAACGATAC ATACTCTTTT AACTGAGGCG 
TTTACTGTAC CAAAGCTTGT TTTAGCTGGT CGACTGCCTT CTCAACAAAA TGCCATTGTT 
AATCTAGATC CAAACATAAG GAATATCCAA GAATTAAAGA CCTGGCCAGA GTTTCATAAC 
GCTGTTGCTG CTGGGTTACG ACTGGCTCCG CTTCAGGGAA AGGTCTCCAG AACGTGGATT 
AGATACAATA AACCTGGAGA GCCAAATGCT GTTCATGCTG GACTTCTGTT TGGCCTGGGA 
TTACAGGGAT ATTTGCATGT GTTAAACCTT AGTGACATAT ACCAATATTT CACTCAGGAC 
CATGAGAGCA CTACGGTAGG TTTGATGCTT GGTCTGGCCG CTTCATACAG GGGAACAATG 
CAACCTGATA TTGCAAAGGC TCTCTTTTTC CATGTTCCTG CTCGATACCA AGCTTCATAT 
ACTGAGTTTG AGATACCAAC ACTTCTACAG TCTGCAGCTT TAGTCTCCGT TGGCATGCTA 
TTTGAAGGAT CAGCACACCA GCAGACAATG CAATTACTTT TGGGTGAAAT TGGCCGTAGA 
AGTGCAGGGG ACAATGTCCT TGAAAGGGAG GGTTATGCTG TATCTGCAGG ATTCTCACTT 
GGTCTTGTAG CTTTGGGCCG TGGAGGTGAT GCGCTCGGTT CCATGGACTC CTTGGTCAAT 
CGCTTACTCC TGTATTTAGG AGCAAAAGAG GAAAGATCTA TCCTTGTACC ATCCCTTGAA 
GATCACCGAA GTGCTGCACA GATAACAGAC GGAAGCACTT CTAATGTTGA TATAACTGCA 
CCTGGAGCGA TAATAGCTCT CACATTAATG TATCTTAAGA CAGAATCAGA GGTCATCTTC 
TCAAAGCTCT CTATTCCTCA AACACATTAC GATTTGGAGT GCGTTAGGCC TGATTTCATT 
ATGCTCCGTG TCATTGCTCG GAACTTGATA ATGTGGAGCA GGATCTGTCC TACTTGTGAT 
TGGATTCAGT CTCAAGTTCC TGAAGTAGTC AAAAATGGTA TCAGCCAACT CCGAGATGAC 
ATGGATAATA TGTATGAGGT GGATGTTGAA GCCCTTGTTC AGGCATACGT CAATATAGTG 
GCCGGGGCCT GCATTTCACT TGGGCTGAGA TTTGCTGGTA CGAGGGATGG AAATGCTCGT 
GACTTACTTA ACTCCTACGC TCTCTATCTT TTGAATGAGA TCAAGCCTCT TTCTGCCACA 
CCTGGAAATG CATTTCCCAG GGGAATATCA AAATTTGTTG ATCGGGGAAC ACTTGAAATG 
TGTCTTTATC TTATCATTAT TTCTCTGTCA GTGGTCATGG CGGGATCTGG AGACTTGCAA 
GTTTTTCGGT TGTTGAGATT TCTCCGCAGC CGAAACTCTG CCGATGGACA TGCTAACTAT 
GGCACACAAA TGGCAGTGAG CTTAGCCACA GGATTCTTGT TTCTTGGAGG CGGTATGCGA 
ACATTTTCAA CGAATAATGG CTCACTGGCA ATGTTGCTCA TTACTCTCTA TCCACGGTTG 
CCTTCTGGAC CAAATGACAA CCGTTGTCAC CTCCAGGCAT TCAGGCATTT ATATGTCCTT 
GCAACAGAGG CTCGCTGGCT GCAGACCATT GATGTTGATT CTGGTCTGCC TGTCTATGCC 
CCTCTAGAAG TCACAGTAAA AGAGACAAAA CTTTATTCAG AAACAAAATT TTGCGAGATA 
ACACCGTGCA TTCTGCCAGA GCGTGCTATT CTAAAGAGAA TTTGTGTATG TGGTCCCCGA 
TACTGGCCCC AACAAATTGA GCTAGTCCCC GAAGAGAAAA ATTGGTGGAG CTTTGGGGAC 
AAGAGTGATC CCTTCAGTTC TGGTGTTATA TATGTCAAAA GGAAAGTAGG AGCTTGTTCG 
TATGTTGATG ATCCAGTTGG GTGTCAGTCA CTGCTTTCGC GAGCAATGCA TAAGGTTTTT 
GGTTTAAGGA CTCTGGGAGA ATCTAATTTG ATTGCTAATA GTCATAGAGA ACTGGATTCT 
GATAGTGTTG ATCACTTGGT CAGCACTTTC TCATCAGATC CAAGCCTAAT AGCGTTTGCA 
CAGTTGTGCT GTGACAAATC TTGGAATAAC AGATCTGATT CTGAATTCAA GGAGTTCTGT 
CTGCAAGTGT TGTTTGACTG TATAAGCAAG GATAGACCAG CTCTTTTGCA GGTTTATTTA 
TCTTTGTACA CAACCATTGG TTCGATGGCT GATCTGCTTG TCAAAAGCGA TAGTAACATG 
TGTGATTCAC TTTCCATCTC AAGCCTCAAG GTTGCTCTTG CCTACAATGA GGCAGTATCA 
ACTGGAAGAT TGGCCTCCTC AGGTGGCTTT GTACAGTCAA TCTTCTTAGC ATCGCTGAGA 
AAGCGATGTG AAGAGGTTTT GAATTGCTCA ACGGAGCTGA AGATTAATTT GAGGAATTAT 
TTGACCTCAG AAGCATGGCC TTATGATAAG AACTCAAAGT TGCAGAAAGA TATAATTATT 
CTCTCATGGT ATCTAAAATG GTTCAACGTA CCATCTCCAT CGATCATCAA AGCAGCAGTG 
GAAAAGATCA AATCCAAGTC CAAAAACTCA ACTTCGGCAA TACCTTTGTT GCGATTGTTG 
TTACCAAATA CGCATATTAG TGTGATTGGT GAAATTGATA GGGTCTTTTT CCCCTCGAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331420.1
CDS	131..5173
Translation	MRTVKSIWPLPCGLLLEQAGEVNPPSHVPFSPVSPILGSREMLRQRKEVGNSSPQNFHSPVAHDLISKRDMPCMSSHLILRDPLEEPGPTYVEERGKLTIMKDYDERTIWTSDRLPLMTSYNKGKMQHSVWAAEFIESNLEASASCSSGIVPDAVLSKRVSFRRIWQAKGAKKAASKVFLATDNSVPVICFLILEQKKLLSVGLQTVEINNEILFDVKPDISWSVSAIAAAPVVVTRSQVKIGLLPHLDIIVLSPENDLFLYSGKQCLCRYVLPSWLGESIGSGDGESAKTDSGFRNLKITGLSDAVLGSINLSVNHSQIFRCALTGKPSSSLANDCIAAIAEGLRSDLYSLFLSLLWGDGHSDLQGSSIHFEWEALCNIFLEICQKPTVVHRKQPKTASESSWEFLLISKFHKTYSRFHNGITSINRLDLEGIVPFDSKICSEETLGSSCELMVQSLDCLHAVYESLKMDNLRKQDLHHLAVLLCNIAKFLDEKCYLDYYIRDFPRLSTTIGACTTLSSSRKPPNLFRWLENCLRRGCLSTNFDDLPDLIRRDGCSIVSWARKVVSFYSVLFGDKPEGRTLSSGVPCNIAPGSYSCNEELTILAMAGERFGLHQLDLLPSGVSLPLRHALDSCRESPPADWPAIAYVLLGREDMALSVFRNFSSSKEFEMQSNTSLISMSIPYMLHLHPVIVPSSESIGLENTKIEDTNSVDGSVIDGMEHIFNSYTQLRYGRDLRLNEVRRLLCSARPVVVQTAANPTISDQEQQQDHLWRIAQRTAVLPLGRGAFTLSTIHTLLTEAFTVPKLVLAGRLPSQQNAIVNLDPNIRNIQELKTWPEFHNAVAAGLRLAPLQGKVSRTWIRYNKPGEPNAVHAGLLFGLGLQGYLHVLNLSDIYQYFTQDHESTTVGLMLGLAASYRGTMQPDIAKALFFHVPARYQASYTEFEIPTLLQSAALVSVGMLFEGSAHQQTMQLLLGEIGRRSAGDNVLEREGYAVSAGFSLGLVALGRGGDALGSMDSLVNRLLLYLGAKEERSILVPSLEDHRSAAQITDGSTSNVDITAPGAIIALTLMYLKTESEVIFSKLSIPQTHYDLECVRPDFIMLRVIARNLIMWSRICPTCDWIQSQVPEVVKNGISQLRDDMDNMYEVDVEALVQAYVNIVAGACISLGLRFAGTRDGNARDLLNSYALYLLNEIKPLSATPGNAFPRGISKFVDRGTLEMCLYLIIISLSVVMAGSGDLQVFRLLRFLRSRNSADGHANYGTQMAVSLATGFLFLGGGMRTFSTNNGSLAMLLITLYPRLPSGPNDNRCHLQAFRHLYVLATEARWLQTIDVDSGLPVYAPLEVTVKETKLYSETKFCEITPCILPERAILKRICVCGPRYWPQQIELVPEEKNWWSFGDKSDPFSSGVIYVKRKVGACSYVDDPVGCQSLLSRAMHKVFGLRTLGESNLIANSHRELDSDSVDHLVSTFSSDPSLIAFAQLCCDKSWNNRSDSEFKEFCLQVLFDCISKDRPALLQVYLSLYTTIGSMADLLVKSDSNMCDSLSISSLKVALAYNEAVSTGRLASSGGFVQSIFLASLRKRCEEVLNCSTELKINLRNYLTSEAWPYDKNSKLQKDIIILSWYLKWFNVPSPSIIKAAVEKIKSKSKNSTSAIPLLRLLLPNTHISVIGEIDRVFFPSN

Target ORF information:

RefSeq Version	NM_001342826.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342826.1

ORF Insert Sequence:

ATGCGTACTG TCAAATCAAT ATGGCCTCTA CCATGTGGTC TACTTCTTGA GCAAGCTGGT 
GAAGTGAATC CACCCTCACA TGTTCCATTT TCTCCTGTTA GCCCCATCCT TGGTTCTCGT 
GAGATGCTGC GCCAAAGAAA AGAAGTTGGG AATAGTTCAC CACAAAACTT CCATTCCCCA 
GTCGCGCATG ACCTTATTAG CAAAAGAGAT ATGCCATGCA TGTCTTCCCA TCTGATTCTA 
AGAGATCCAC TAGAGGAACC TGGGCCAACC TACGTTGAAG AGAGGGGGAA GTTGACCATA 
ATGAAGGACT ACGATGAGAG GACAATTTGG ACAAGTGACC GTCTACCTCT TATGACGTCG 
TATAACAAAG GCAAGATGCA ACATTCTGTG TGGGCGGCCG AATTTATAGA GTCAAACCTT 
GAAGCTTCTG CGTCATGTTC AAGCGGCATT GTTCCTGATG CTGTTCTCTC GAAGCGGGTT 
TCTTTTCGTA GAATATGGCA AGCGAAAGGT GCTAAGAAAG CTGCATCTAA GGTCTTTTTG 
GCAACTGATA ATTCAGTCCC AGTAATTTGC TTTCTGATTC TAGAACAAAA GAAGCTGTTA 
TCGGTGGGAC TTCAAACTGT TGAAATCAAT AACGAAATTC TGTTCGATGT TAAGCCTGAT 
ATAAGCTGGA GTGTGTCTGC GATTGCTGCT GCACCTGTTG TTGTGACACG TTCTCAAGTG 
AAAATAGGAC TGCTTCCACA TTTGGATATC ATTGTTTTAT CTCCAGAAAA TGACCTCTTT 
CTCTATTCAG GAAAACAATG TCTCTGTAGA TATGTATTAC CTTCTTGGTT GGGAGAAAGT 
ATTGGTTCCG GGGATGGAGA GTCTGCAAAA ACAGATTCAG GTTTCAGGAA CCTGAAGATC 
ACAGGATTGT CTGATGCTGT TTTAGGATCT ATCAATCTCT CAGTTAACCA TTCACAGATC 
TTTCGGTGTG CCTTAACTGG TAAACCTTCA TCGTCACTTG CAAATGATTG CATAGCAGCC 
ATAGCTGAGG GACTACGATC CGATCTGTAC AGTTTGTTTC TCTCTCTTCT TTGGGGAGAT 
GGTCATTCTG ACCTGCAAGG TTCCAGTATT CATTTTGAAT GGGAAGCATT ATGCAACATT 
TTTCTGGAGA TCTGTCAAAA GCCTACTGTT GTGCATCGAA AGCAACCCAA AACAGCATCA 
GAGTCCTCCT GGGAGTTTCT TCTCATCAGC AAATTTCATA AGACCTACTC TAGGTTTCAC 
AATGGTATTA CTTCAATAAA TCGTTTAGAT CTGGAAGGCA TTGTCCCATT TGATTCCAAG 
ATTTGTAGTG AAGAAACACT GGGCAGCTCA TGTGAACTAA TGGTCCAGAG TTTAGATTGT 
CTCCATGCAG TTTATGAGAG CTTGAAGATG GATAATCTAC GGAAACAGGA TTTGCATCAC 
TTGGCTGTTT TGTTGTGCAA TATTGCCAAG TTTCTGGATG AGAAGTGTTA CTTAGATTAC 
TACATACGTG ATTTTCCTCG TCTGTCAACA ACTATTGGGG CGTGCACAAC CCTTTCTTCT 
AGCAGAAAAC CTCCAAATTT ATTTAGATGG CTTGAGAATT GTTTAAGACG TGGATGTTTA 
TCAACAAACT TTGATGACCT CCCAGATTTG ATCCGTAGAG ATGGGTGCTC CATAGTAAGC 
TGGGCAAGGA AAGTTGTTTC CTTCTACAGT GTGTTATTTG GTGATAAGCC AGAAGGACGG 
ACACTTTCAT CAGGTGTCCC CTGTAATATC GCGCCAGGAT CATACTCCTG TAACGAGGAA 
CTCACTATCT TAGCTATGGC AGGAGAGAGA TTTGGGCTTC ACCAACTGGA TTTGCTACCT 
TCTGGTGTTT CTCTCCCGCT AAGACATGCA CTGGATAGCT GTCGGGAATC TCCTCCAGCT 
GACTGGCCAG CAATTGCTTA TGTGCTTCTT GGTCGAGAAG ATATGGCCCT ATCCGTCTTC 
AGAAATTTTA GTTCATCTAA GGAATTTGAG ATGCAGTCAA ATACGAGTTT GATATCGATG 
TCCATACCTT ACATGCTACA TTTGCATCCA GTAATTGTTC CATCCTCTGA GTCGATTGGC 
TTAGAAAACA CTAAGATTGA GGATACAAAT TCTGTGGATG GTTCTGTGAT AGATGGAATG 
GAGCATATCT TCAACTCCTA CACACAGCTA CGGTATGGCC GGGATCTAAG ATTGAATGAG 
GTTAGACGTC TTTTGTGCTC TGCAAGACCT GTGGTAGTTC AAACGGCTGC TAACCCTACT 
ATATCAGATC AGGAGCAGCA ACAGGACCAT CTTTGGCGCA TAGCACAGAG GACTGCTGTT 
CTACCCCTCG GGCGTGGAGC ATTCACATTA TCAACGATAC ATACTCTTTT AACTGAGGCG 
TTTACTGTAC CAAAGCTTGT TTTAGCTGGT CGACTGCCTT CTCAACAAAA TGCCATTGTT 
AATCTAGATC CAAACATAAG GAATATCCAA GAATTAAAGA CCTGGCCAGA GTTTCATAAC 
GCTGTTGCTG CTGGGTTACG ACTGGCTCCG CTTCAGGGAA AGGTCTCCAG AACGTGGATT 
AGATACAATA AACCTGGAGA GCCAAATGCT GTTCATGCTG GACTTCTGTT TGGCCTGGGA 
TTACAGGGAT ATTTGCATGT GTTAAACCTT AGTGACATAT ACCAATATTT CACTCAGGAC 
CATGAGAGCA CTACGGTAGG TTTGATGCTT GGTCTGGCCG CTTCATACAG GGGAACAATG 
CAACCTGATA TTGCAAAGGC TCTCTTTTTC CATGTTCCTG CTCGATACCA AGCTTCATAT 
ACTGAGTTTG AGATACCAAC ACTTCTACAG TCTGCAGCTT TAGTCTCCGT TGGCATGCTA 
TTTGAAGGAT CAGCACACCA GCAGACAATG CAATTACTTT TGGGTGAAAT TGGCCGTAGA 
AGTGCAGGGG ACAATGTCCT TGAAAGGGAG GGTTATGCTG TATCTGCAGG ATTCTCACTT 
GGTCTTGTAG CTTTGGGCCG TGGAGGTGAT GCGCTCGGTT CCATGGACTC CTTGGTCAAT 
CGCTTACTCC TGTATTTAGG AGCAAAAGAG GAAAGATCTA TCCTTGTACC ATCCCTTGAA 
GATCACCGAA GTGCTGCACA GATAACAGAC GGAAGCACTT CTAATGTTGA TATAACTGCA 
CCTGGAGCGA TAATAGCTCT CACATTAATG TATCTTAAGA CAGAATCAGA GGTCATCTTC 
TCAAAGCTCT CTATTCCTCA AACACATTAC GATTTGGAGT GCGTTAGGCC TGATTTCATT 
ATGCTCCGTG TCATTGCTCG GAACTTGATA ATGTGGAGCA GGATCTGTCC TACTTGTGAT 
TGGATTCAGT CTCAAGTTCC TGAAGTAGTC AAAAATGGTA TCAGCCAACT CCGAGATGAC 
ATGGATAATA TGTATGAGGT GGATGTTGAA GCCCTTGTTC AGGCATACGT CAATATAGTG 
GCCGGGGCCT GCATTTCACT TGGGCTGAGA TTTGCTGGTA CGAGGGATGG AAATGCTCGT 
GACTTACTTA ACTCCTACGC TCTCTATCTT TTGAATGAGA TCAAGCCTCT TTCTGCCACA 
CCTGGAAATG CATTTCCCAG GGGAATATCA AAATTTGTTG ATCGGGGAAC ACTTGAAATG 
TGTCTTTATC TTATCATTAT TTCTCTGTCA GTGGTCATGG CGGGATCTGG AGACTTGCAA 
GTTTTTCGGT TGTTGAGATT TCTCCGCAGC CGAAACTCTG CCGATGGACA TGCTAACTAT 
GGCACACAAA TGGCAGTGAG CTTAGCCACA GGATTCTTGT TTCTTGGAGG CGGTATGCGA 
ACATTTTCAA CGAATAATGG CTCACTGGCA ATGTTGCTCA TTACTCTCTA TCCACGGTTG 
CCTTCTGGAC CAAATGACAA CCGTTGTCAC CTCCAGGCAT TCAGGCATTT ATATGTCCTT 
GCAACAGAGG CTCGCTGGCT GCAGACCATT GATGTTGATT CTGGTCTGCC TGTCTATGCC 
CCTCTAGAAG TCACAGTAAA AGAGACAAAA CTTTATTCAG AAACAAAATT TTGCGAGATA 
ACACCGTGCA TTCTGCCAGA GCGTGCTATT CTAAAGAGAA TTTGTGTATG TGGTCCCCGA 
TACTGGCCCC AACAAATTGA GCTAGTCCCC GAAGAGAAAA ATTGGTGGAG CTTTGGGGAC 
AAGAGTGATC CCTTCAGTTC TGGTGTTATA TATGTCAAAA GGAAAGTAGG AGCTTGTTCG 
TATGTTGATG ATCCAGTTGG GTGTCAGTCA CTGCTTTCGC GAGCAATGCA TAAGGTTTTT 
GGTTTAAGGA CTCTGGGAGA ATCTAATTTG ATTGCTAATA GTCATAGAGA ACTGGATTCT 
GATAGTGTTG ATCACTTGGT CAGCACTTTC TCATCAGATC CAAGCCTAAT AGCGTTTGCA 
CAGTTGTGCT GTGACAAATC TTGGAATAAC AGATCTGATT CTGAATTCAA GGAGTTCTGT 
CTGCAAGTGT TGTTTGACTG TATAAGCAAG GATAGACCAG CTCTTTTGCA GGTTTATTTA 
TCTTTGTACA CAACCATTGG TTCGATGGCT GATCTGCTTG TCAAAAGCGA TAGTAACATG 
TGTGATTCAC TTTCCATCTC AAGCCTCAAG GTTGCTCTTG CCTACAATGA GGCAGTATCA 
ACTGGAAGAT TGGCCTCCTC AGGTGGCTTT GTACAGTCAA TCTTCTTAGC ATCGCTGAGA 
AAGCGATGTG AAGAGGTTTT GAATTGCTCA ACGGAGCTGA AGATTAATTT GAGGAATTAT 
TTGACCTCAG AAGCATGGCC TTATGATAAG AACTCAAAGT TGCAGAAAGA TATAATTATT 
CTCTCATGGT ATCTAAAATG GTTCAACGTA CCATCTCCAT CGATCATCAA AGCAGCAGTG 
GAAAAGATCA AATCCAAGTC CAAAAACTCA ACTTCGGCAA TACCTTTGTT GCGATTGTTG 
TTACCAAATA CGCATATTAG TGTGATTGGT GAAATTGATA GGGTCTTTTT CCCCTCGAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMB2771 ( NM_001342825.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331419.1 Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.

Price & Availability↑

CloneID	OAb40586
Clone ID Related Accession (Same CDS sequence)	NM_001342825.1
Accession Version	NM_001342825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	E3 ubiquitin ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGCCTCCAG GGGTTCGCCA ACTTACCGTC CTCGGAAAGT TTAAACCTTT CGGTCTAATC 
GCCGAAGCTA CTGATGGTAA ATCACCGGAT GATTCCTATC AGTATTTCCT CTTCGATCCT 
GAGCTCACTG GCGAGCGAGA TGATGCTGAT GGCAATGATG CCAACTTCTC GCGGCAGAGA 
GAGCACGAGC TCTTCATCCG AGACAACTGC ATAATCTGGA CAAGCGGTTC GCGGGTCCTC 
AAGCGTTTTA CCTTGTCTTC TGCCGTCATT AAGGCATGCT GGAGCAACCT TGGTCGAGGG 
ACAGAAGCTT TTCTTTGTGT ATTACAAATT GCTTGCTTAA CAATCTACAA CACATCAGGC 
GAAGTTGTAT CTGTTCCACT AATGCGTACT GTCAAATCAA TATGGCCTCT ACCATGTGGT 
CTACTTCTTG AGCAAGCTGG TGAAGTGAAT CCACCCTCAC ATGTTCCATT TTCTCCTGTT 
AGCCCCATCC TTGGTTCTCG TGAGATGCTG CGCCAAAGAA AAGAAGTTGG GAATAGTTCA 
CCACAAAACT TCCATTCCCC AGTCGCGCAT GACCTTATTA GCAAAAGAGA TATGCCATGC 
ATGTCTTCCC ATCTGATTCT AAGAGATCCA CTAGAGGAAC CTGGGCCAAC CTACGTTGAA 
GAGAGGGGGA AGTTGACCAT AATGAAGGAC TACGATGAGA GGACAATTTG GACAAGTGAC 
CGTCTACCTC TTATGACGTC GTATAACAAA GGCAAGATGC AACATTCTGT GTGGGCGGCC 
GAATTTATAG AGTCAAACCT TGAAGCTTCT GCGTCATGTT CAAGCGGCAT TGTTCCTGAT 
GCTGTTCTCT CGAAGCGGGT TTCTTTTCGT AGAATATGGC AAGCGAAAGG TGCTAAGAAA 
GCTGCATCTA AGGTCTTTTT GGCAACTGAT AATTCAGTCC CAGTAATTTG CTTTCTGATT 
CTAGAACAAA AGAAGCTGTT ATCGGTGGGA CTTCAAACTG TTGAAATCAA TAACGAAATT 
CTGTTCGATG TTAAGCCTGA TATAAGCTGG AGTGTGTCTG CGATTGCTGC TGCACCTGTT 
GTTGTGACAC GTTCTCAAGT GAAAATAGGA CTGCTTCCAC ATTTGGATAT CATTGTTTTA 
TCTCCAGAAA ATGACCTCTT TCTCTATTCA GGAAAACAAT GTCTCTGTAG ATATGTATTA 
CCTTCTTGGT TGGGAGAAAG TATTGGTTCC GGGGATGGAG AGTCTGCAAA AACAGATTCA 
GGTTTCAGGA ACCTGAAGAT CACAGGATTG TCTGATGCTG TTTTAGGATC TATCAATCTC 
TCAGTTAACC ATTCACAGAT CTTTCGGTGT GCCTTAACTG GTAAACCTTC ATCGTCACTT 
GCAAATGATT GCATAGCAGC CATAGCTGAG GGACTACGAT CCGATCTGTA CAGTTTGTTT 
CTCTCTCTTC TTTGGGGAGA TGGTCATTCT GACCTGCAAG GTTCCAGTAT TCATTTTGAA 
TGGGAAGCAT TATGCAACAT TTTTCTGGAG ATCTGTCAAA AGCCTACTGT TGTGCATCGA 
AAGCAACCCA AAACAGCATC AGAGTCCTCC TGGGAGTTTC TTCTCATCAG CAAATTTCAT 
AAGACCTACT CTAGGTTTCA CAATGGTATT ACTTCAATAA ATCGTTTAGA TCTGGAAGGC 
ATTGTCCCAT TTGATTCCAA GATTTGTAGT GAAGAAACAC TGGGCAGCTC ATGTGAACTA 
ATGGTCCAGA GTTTAGATTG TCTCCATGCA GTTTATGAGA GCTTGAAGAT GGATAATCTA 
CGGAAACAGG ATTTGCATCA CTTGGCTGTT TTGTTGTGCA ATATTGCCAA GTTTCTGGAT 
GAGAAGTGTT ACTTAGATTA CTACATACGT GATTTTCCTC GTCTGTCAAC AACTATTGGG 
GCGTGCACAA CCCTTTCTTC TAGCAGAAAA CCTCCAAATT TATTTAGATG GCTTGAGAAT 
TGTTTAAGAC GTGGATGTTT ATCAACAAAC TTTGATGACC TCCCAGATTT GATCCGTAGA 
GATGGGTGCT CCATAGTAAG CTGGGCAAGG AAAGTTGTTT CCTTCTACAG TGTGTTATTT 
GGTGATAAGC CAGAAGGACG GACACTTTCA TCAGGTGTCC CCTGTAATAT CGCGCCAGGA 
TCATACTCCT GTAACGAGGA ACTCACTATC TTAGCTATGG CAGGAGAGAG ATTTGGGCTT 
CACCAACTGG ATTTGCTACC TTCTGGTGTT TCTCTCCCGC TAAGACATGC ACTGGATAGC 
TGTCGGGAAT CTCCTCCAGC TGACTGGCCA GCAATTGCTT ATGTGCTTCT TGGTCGAGAA 
GATATGGCCC TATCCGTCTT CAGAAATTTT AGTTCATCTA AGGAATTTGA GATGCAGTCA 
AATACGAGTT TGATATCGAT GTCCATACCT TACATGCTAC ATTTGCATCC AGTAATTGTT 
CCATCCTCTG AGTCGATTGG CTTAGAAAAC ACTAAGATTG AGGATACAAA TTCTGTGGAT 
GGTTCTGTGA TAGATGGAAT GGAGCATATC TTCAACTCCT ACACACAGCT ACGGTATGGC 
CGGGATCTAA GATTGAATGA GGTTAGACGT CTTTTGTGCT CTGCAAGACC TGTGGTAGTT 
CAAACGGCTG CTAACCCTAC TATATCAGAT CAGGAGCAGC AACAGGACCA TCTTTGGCGC 
ATAGCACAGA GGACTGCTGT TCTACCCCTC GGGCGTGGAG CATTCACATT ATCAACGATA 
CATACTCTTT TAACTGAGGC GTTTACTGTA CCAAAGCTTG TTTTAGCTGG TCGACTGCCT 
TCTCAACAAA ATGCCATTGT TAATCTAGAT CCAAACATAA GGAATATCCA AGAATTAAAG 
ACCTGGCCAG AGTTTCATAA CGCTGTTGCT GCTGGGTTAC GACTGGCTCC GCTTCAGGGA 
AAGGTCTCCA GAACGTGGAT TAGATACAAT AAACCTGGAG AGCCAAATGC TGTTCATGCT 
GGACTTCTGT TTGGCCTGGG ATTACAGGGA TATTTGCATG TGTTAAACCT TAGTGACATA 
TACCAATATT TCACTCAGGA CCATGAGAGC ACTACGGTAG GTTTGATGCT TGGTCTGGCC 
GCTTCATACA GGGGAACAAT GCAACCTGAT ATTGCAAAGG CTCTCTTTTT CCATGTTCCT 
GCTCGATACC AAGCTTCATA TACTGAGTTT GAGATACCAA CACTTCTACA GTCTGCAGCT 
TTAGTCTCCG TTGGCATGCT ATTTGAAGGA TCAGCACACC AGCAGACAAT GCAATTACTT 
TTGGGTGAAA TTGGCCGTAG AAGTGCAGGG GACAATGTCC TTGAAAGGGA GGGTTATGCT 
GTATCTGCAG GATTCTCACT TGGTCTTGTA GCTTTGGGCC GTGGAGGTGA TGCGCTCGGT 
TCCATGGACT CCTTGGTCAA TCGCTTACTC CTGTATTTAG GAGCAAAAGA GGAAAGATCT 
ATCCTTGTAC CATCCCTTGA AGATCACCGA AGTGCTGCAC AGATAACAGA CGGAAGCACT 
TCTAATGTTG ATATAACTGC ACCTGGAGCG ATAATAGCTC TCACATTAAT GTATCTTAAG 
ACAGAATCAG AGGTCATCTT CTCAAAGCTC TCTATTCCTC AAACACATTA CGATTTGGAG 
TGCGTTAGGC CTGATTTCAT TATGCTCCGT GTCATTGCTC GGAACTTGAT AATGTGGAGC 
AGGATCTGTC CTACTTGTGA TTGGATTCAG TCTCAAGTTC CTGAAGTAGT CAAAAATGGT 
ATCAGCCAAC TCCGAGATGA CATGGATAAT ATGTATGAGG TGGATGTTGA AGCCCTTGTT 
CAGGCATACG TCAATATAGT GGCCGGGGCC TGCATTTCAC TTGGGCTGAG ATTTGCTGGT 
ACGAGGGATG GAAATGCTCG TGACTTACTT AACTCCTACG CTCTCTATCT TTTGAATGAG 
ATCAAGCCTC TTTCTGCCAC ACCTGGAAAT GCATTTCCCA GGGGAATATC AAAATTTGTT 
GATCGGGGAA CACTTGAAAT GTGTCTTTAT CTTATCATTA TTTCTCTGTC AGTGGTCATG 
GCGGGATCTG GAGACTTGCA AGTTTTTCGG TTGTTGAGAT TTCTCCGCAG CCGAAACTCT 
GCCGATGGAC ATGCTAACTA TGGCACACAA ATGGCAGTGA GCTTAGCCAC AGGATTCTTG 
TTTCTTGGAG GCGGTATGCG AACATTTTCA ACGAATAATG GCTCACTGGC AATGTTGCTC 
ATTACTCTCT ATCCACGGTT GCCTTCTGGA CCAAATGACA ACCGTTGTCA CCTCCAGGCA 
TTCAGGCATT TATATGTCCT TGCAACAGAG GCTCGCTGGC TGCAGACCAT TGATGTTGAT 
TCTGGTCTGC CTGTCTATGC CCCTCTAGAA GTCACAGTAA AAGAGACAAA ACTTTATTCA 
GAAACAAAAT TTTGCGAGAT AACACCGTGC ATTCTGCCAG AGCGTGCTAT TCTAAAGAGA 
ATTTGTGTAT GTGGTCCCCG ATACTGGCCC CAACAAATTG AGCTAGTCCC CGAAGAGAAA 
AATTGGTGGA GCTTTGGGGA CAAGAGTGAT CCCTTCAGTT CTGGTGTTAT ATATGTCAAA 
AGGAAAGTAG GAGCTTGTTC GTATGTTGAT GATCCAGTTG GGTGTCAGTC ACTGCTTTCG 
CGAGCAATGC ATAAGGTTTT TGGTTTAAGG ACTCTGGGAG AATCTAATTT GATTGCTAAT 
AGTCATAGAG AACTGGATTC TGATAGTGTT GATCACTTGG TCAGCACTTT CTCATCAGAT 
CCAAGCCTAA TAGCGTTTGC ACAGTTGTGC TGTGACAAAT CTTGGAATAA CAGATCTGAT 
TCTGAATTCA AGGAGTTCTG TCTGCAAGTG TTGTTTGACT GTATAAGCAA GGATAGACCA 
GCTCTTTTGC AGGTTTATTT ATCTTTGTAC ACAACCATTG GTTCGATGGC TGATCTGCTT 
GTCAAAAGCG ATAGTAACAT GTGTGATTCA CTTTCCATCT CAAGCCTCAA GGTTGCTCTT 
GCCTACAATG AGGCAGTATC AACTGGAAGA TTGGCCTCCT CAGGTGGCTT TGTACAGTCA 
ATCTTCTTAG CATCGCTGAG AAAGCGATGT GAAGAGGTTT TGAATTGCTC AACGGAGCTG 
AAGATTAATT TGAGGAATTA TTTGACCTCA GAAGCATGGC CTTATGATAA GAACTCAAAG 
TTGCAGAAAG ATATAATTAT TCTCTCATGG TATCTAAAAT GGTTCAACGT ACCATCTCCA 
TCGATCATCA AAGCAGCAGT GGAAAAGATC AAATCCAAGT CCAAAAACTC AACTTCGGCA 
ATACCTTTGT TGCGATTGTT GTTACCAAAT ACGCATATTA GTGTGATTGG TGAAATTGAT 
AGGGTCTTTT TCCCCTCGAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331419.1
CDS	1246..6669
Translation	MPPGVRQLTVLGKFKPFGLIAEATDGKSPDDSYQYFLFDPELTGERDDADGNDANFSRQREHELFIRDNCIIWTSGSRVLKRFTLSSAVIKACWSNLGRGTEAFLCVLQIACLTIYNTSGEVVSVPLMRTVKSIWPLPCGLLLEQAGEVNPPSHVPFSPVSPILGSREMLRQRKEVGNSSPQNFHSPVAHDLISKRDMPCMSSHLILRDPLEEPGPTYVEERGKLTIMKDYDERTIWTSDRLPLMTSYNKGKMQHSVWAAEFIESNLEASASCSSGIVPDAVLSKRVSFRRIWQAKGAKKAASKVFLATDNSVPVICFLILEQKKLLSVGLQTVEINNEILFDVKPDISWSVSAIAAAPVVVTRSQVKIGLLPHLDIIVLSPENDLFLYSGKQCLCRYVLPSWLGESIGSGDGESAKTDSGFRNLKITGLSDAVLGSINLSVNHSQIFRCALTGKPSSSLANDCIAAIAEGLRSDLYSLFLSLLWGDGHSDLQGSSIHFEWEALCNIFLEICQKPTVVHRKQPKTASESSWEFLLISKFHKTYSRFHNGITSINRLDLEGIVPFDSKICSEETLGSSCELMVQSLDCLHAVYESLKMDNLRKQDLHHLAVLLCNIAKFLDEKCYLDYYIRDFPRLSTTIGACTTLSSSRKPPNLFRWLENCLRRGCLSTNFDDLPDLIRRDGCSIVSWARKVVSFYSVLFGDKPEGRTLSSGVPCNIAPGSYSCNEELTILAMAGERFGLHQLDLLPSGVSLPLRHALDSCRESPPADWPAIAYVLLGREDMALSVFRNFSSSKEFEMQSNTSLISMSIPYMLHLHPVIVPSSESIGLENTKIEDTNSVDGSVIDGMEHIFNSYTQLRYGRDLRLNEVRRLLCSARPVVVQTAANPTISDQEQQQDHLWRIAQRTAVLPLGRGAFTLSTIHTLLTEAFTVPKLVLAGRLPSQQNAIVNLDPNIRNIQELKTWPEFHNAVAAGLRLAPLQGKVSRTWIRYNKPGEPNAVHAGLLFGLGLQGYLHVLNLSDIYQYFTQDHESTTVGLMLGLAASYRGTMQPDIAKALFFHVPARYQASYTEFEIPTLLQSAALVSVGMLFEGSAHQQTMQLLLGEIGRRSAGDNVLEREGYAVSAGFSLGLVALGRGGDALGSMDSLVNRLLLYLGAKEERSILVPSLEDHRSAAQITDGSTSNVDITAPGAIIALTLMYLKTESEVIFSKLSIPQTHYDLECVRPDFIMLRVIARNLIMWSRICPTCDWIQSQVPEVVKNGISQLRDDMDNMYEVDVEALVQAYVNIVAGACISLGLRFAGTRDGNARDLLNSYALYLLNEIKPLSATPGNAFPRGISKFVDRGTLEMCLYLIIISLSVVMAGSGDLQVFRLLRFLRSRNSADGHANYGTQMAVSLATGFLFLGGGMRTFSTNNGSLAMLLITLYPRLPSGPNDNRCHLQAFRHLYVLATEARWLQTIDVDSGLPVYAPLEVTVKETKLYSETKFCEITPCILPERAILKRICVCGPRYWPQQIELVPEEKNWWSFGDKSDPFSSGVIYVKRKVGACSYVDDPVGCQSLLSRAMHKVFGLRTLGESNLIANSHRELDSDSVDHLVSTFSSDPSLIAFAQLCCDKSWNNRSDSEFKEFCLQVLFDCISKDRPALLQVYLSLYTTIGSMADLLVKSDSNMCDSLSISSLKVALAYNEAVSTGRLASSGGFVQSIFLASLRKRCEEVLNCSTELKINLRNYLTSEAWPYDKNSKLQKDIIILSWYLKWFNVPSPSIIKAAVEKIKSKSKNSTSAIPLLRLLLPNTHISVIGEIDRVFFPSN

Target ORF information:

RefSeq Version	NM_001342825.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana E3 ubiquitin ligase (EMB2771), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342825.1

ORF Insert Sequence:

ATGCCTCCAG GGGTTCGCCA ACTTACCGTC CTCGGAAAGT TTAAACCTTT CGGTCTAATC 
GCCGAAGCTA CTGATGGTAA ATCACCGGAT GATTCCTATC AGTATTTCCT CTTCGATCCT 
GAGCTCACTG GCGAGCGAGA TGATGCTGAT GGCAATGATG CCAACTTCTC GCGGCAGAGA 
GAGCACGAGC TCTTCATCCG AGACAACTGC ATAATCTGGA CAAGCGGTTC GCGGGTCCTC 
AAGCGTTTTA CCTTGTCTTC TGCCGTCATT AAGGCATGCT GGAGCAACCT TGGTCGAGGG 
ACAGAAGCTT TTCTTTGTGT ATTACAAATT GCTTGCTTAA CAATCTACAA CACATCAGGC 
GAAGTTGTAT CTGTTCCACT AATGCGTACT GTCAAATCAA TATGGCCTCT ACCATGTGGT 
CTACTTCTTG AGCAAGCTGG TGAAGTGAAT CCACCCTCAC ATGTTCCATT TTCTCCTGTT 
AGCCCCATCC TTGGTTCTCG TGAGATGCTG CGCCAAAGAA AAGAAGTTGG GAATAGTTCA 
CCACAAAACT TCCATTCCCC AGTCGCGCAT GACCTTATTA GCAAAAGAGA TATGCCATGC 
ATGTCTTCCC ATCTGATTCT AAGAGATCCA CTAGAGGAAC CTGGGCCAAC CTACGTTGAA 
GAGAGGGGGA AGTTGACCAT AATGAAGGAC TACGATGAGA GGACAATTTG GACAAGTGAC 
CGTCTACCTC TTATGACGTC GTATAACAAA GGCAAGATGC AACATTCTGT GTGGGCGGCC 
GAATTTATAG AGTCAAACCT TGAAGCTTCT GCGTCATGTT CAAGCGGCAT TGTTCCTGAT 
GCTGTTCTCT CGAAGCGGGT TTCTTTTCGT AGAATATGGC AAGCGAAAGG TGCTAAGAAA 
GCTGCATCTA AGGTCTTTTT GGCAACTGAT AATTCAGTCC CAGTAATTTG CTTTCTGATT 
CTAGAACAAA AGAAGCTGTT ATCGGTGGGA CTTCAAACTG TTGAAATCAA TAACGAAATT 
CTGTTCGATG TTAAGCCTGA TATAAGCTGG AGTGTGTCTG CGATTGCTGC TGCACCTGTT 
GTTGTGACAC GTTCTCAAGT GAAAATAGGA CTGCTTCCAC ATTTGGATAT CATTGTTTTA 
TCTCCAGAAA ATGACCTCTT TCTCTATTCA GGAAAACAAT GTCTCTGTAG ATATGTATTA 
CCTTCTTGGT TGGGAGAAAG TATTGGTTCC GGGGATGGAG AGTCTGCAAA AACAGATTCA 
GGTTTCAGGA ACCTGAAGAT CACAGGATTG TCTGATGCTG TTTTAGGATC TATCAATCTC 
TCAGTTAACC ATTCACAGAT CTTTCGGTGT GCCTTAACTG GTAAACCTTC ATCGTCACTT 
GCAAATGATT GCATAGCAGC CATAGCTGAG GGACTACGAT CCGATCTGTA CAGTTTGTTT 
CTCTCTCTTC TTTGGGGAGA TGGTCATTCT GACCTGCAAG GTTCCAGTAT TCATTTTGAA 
TGGGAAGCAT TATGCAACAT TTTTCTGGAG ATCTGTCAAA AGCCTACTGT TGTGCATCGA 
AAGCAACCCA AAACAGCATC AGAGTCCTCC TGGGAGTTTC TTCTCATCAG CAAATTTCAT 
AAGACCTACT CTAGGTTTCA CAATGGTATT ACTTCAATAA ATCGTTTAGA TCTGGAAGGC 
ATTGTCCCAT TTGATTCCAA GATTTGTAGT GAAGAAACAC TGGGCAGCTC ATGTGAACTA 
ATGGTCCAGA GTTTAGATTG TCTCCATGCA GTTTATGAGA GCTTGAAGAT GGATAATCTA 
CGGAAACAGG ATTTGCATCA CTTGGCTGTT TTGTTGTGCA ATATTGCCAA GTTTCTGGAT 
GAGAAGTGTT ACTTAGATTA CTACATACGT GATTTTCCTC GTCTGTCAAC AACTATTGGG 
GCGTGCACAA CCCTTTCTTC TAGCAGAAAA CCTCCAAATT TATTTAGATG GCTTGAGAAT 
TGTTTAAGAC GTGGATGTTT ATCAACAAAC TTTGATGACC TCCCAGATTT GATCCGTAGA 
GATGGGTGCT CCATAGTAAG CTGGGCAAGG AAAGTTGTTT CCTTCTACAG TGTGTTATTT 
GGTGATAAGC CAGAAGGACG GACACTTTCA TCAGGTGTCC CCTGTAATAT CGCGCCAGGA 
TCATACTCCT GTAACGAGGA ACTCACTATC TTAGCTATGG CAGGAGAGAG ATTTGGGCTT 
CACCAACTGG ATTTGCTACC TTCTGGTGTT TCTCTCCCGC TAAGACATGC ACTGGATAGC 
TGTCGGGAAT CTCCTCCAGC TGACTGGCCA GCAATTGCTT ATGTGCTTCT TGGTCGAGAA 
GATATGGCCC TATCCGTCTT CAGAAATTTT AGTTCATCTA AGGAATTTGA GATGCAGTCA 
AATACGAGTT TGATATCGAT GTCCATACCT TACATGCTAC ATTTGCATCC AGTAATTGTT 
CCATCCTCTG AGTCGATTGG CTTAGAAAAC ACTAAGATTG AGGATACAAA TTCTGTGGAT 
GGTTCTGTGA TAGATGGAAT GGAGCATATC TTCAACTCCT ACACACAGCT ACGGTATGGC 
CGGGATCTAA GATTGAATGA GGTTAGACGT CTTTTGTGCT CTGCAAGACC TGTGGTAGTT 
CAAACGGCTG CTAACCCTAC TATATCAGAT CAGGAGCAGC AACAGGACCA TCTTTGGCGC 
ATAGCACAGA GGACTGCTGT TCTACCCCTC GGGCGTGGAG CATTCACATT ATCAACGATA 
CATACTCTTT TAACTGAGGC GTTTACTGTA CCAAAGCTTG TTTTAGCTGG TCGACTGCCT 
TCTCAACAAA ATGCCATTGT TAATCTAGAT CCAAACATAA GGAATATCCA AGAATTAAAG 
ACCTGGCCAG AGTTTCATAA CGCTGTTGCT GCTGGGTTAC GACTGGCTCC GCTTCAGGGA 
AAGGTCTCCA GAACGTGGAT TAGATACAAT AAACCTGGAG AGCCAAATGC TGTTCATGCT 
GGACTTCTGT TTGGCCTGGG ATTACAGGGA TATTTGCATG TGTTAAACCT TAGTGACATA 
TACCAATATT TCACTCAGGA CCATGAGAGC ACTACGGTAG GTTTGATGCT TGGTCTGGCC 
GCTTCATACA GGGGAACAAT GCAACCTGAT ATTGCAAAGG CTCTCTTTTT CCATGTTCCT 
GCTCGATACC AAGCTTCATA TACTGAGTTT GAGATACCAA CACTTCTACA GTCTGCAGCT 
TTAGTCTCCG TTGGCATGCT ATTTGAAGGA TCAGCACACC AGCAGACAAT GCAATTACTT 
TTGGGTGAAA TTGGCCGTAG AAGTGCAGGG GACAATGTCC TTGAAAGGGA GGGTTATGCT 
GTATCTGCAG GATTCTCACT TGGTCTTGTA GCTTTGGGCC GTGGAGGTGA TGCGCTCGGT 
TCCATGGACT CCTTGGTCAA TCGCTTACTC CTGTATTTAG GAGCAAAAGA GGAAAGATCT 
ATCCTTGTAC CATCCCTTGA AGATCACCGA AGTGCTGCAC AGATAACAGA CGGAAGCACT 
TCTAATGTTG ATATAACTGC ACCTGGAGCG ATAATAGCTC TCACATTAAT GTATCTTAAG 
ACAGAATCAG AGGTCATCTT CTCAAAGCTC TCTATTCCTC AAACACATTA CGATTTGGAG 
TGCGTTAGGC CTGATTTCAT TATGCTCCGT GTCATTGCTC GGAACTTGAT AATGTGGAGC 
AGGATCTGTC CTACTTGTGA TTGGATTCAG TCTCAAGTTC CTGAAGTAGT CAAAAATGGT 
ATCAGCCAAC TCCGAGATGA CATGGATAAT ATGTATGAGG TGGATGTTGA AGCCCTTGTT 
CAGGCATACG TCAATATAGT GGCCGGGGCC TGCATTTCAC TTGGGCTGAG ATTTGCTGGT 
ACGAGGGATG GAAATGCTCG TGACTTACTT AACTCCTACG CTCTCTATCT TTTGAATGAG 
ATCAAGCCTC TTTCTGCCAC ACCTGGAAAT GCATTTCCCA GGGGAATATC AAAATTTGTT 
GATCGGGGAA CACTTGAAAT GTGTCTTTAT CTTATCATTA TTTCTCTGTC AGTGGTCATG 
GCGGGATCTG GAGACTTGCA AGTTTTTCGG TTGTTGAGAT TTCTCCGCAG CCGAAACTCT 
GCCGATGGAC ATGCTAACTA TGGCACACAA ATGGCAGTGA GCTTAGCCAC AGGATTCTTG 
TTTCTTGGAG GCGGTATGCG AACATTTTCA ACGAATAATG GCTCACTGGC AATGTTGCTC 
ATTACTCTCT ATCCACGGTT GCCTTCTGGA CCAAATGACA ACCGTTGTCA CCTCCAGGCA 
TTCAGGCATT TATATGTCCT TGCAACAGAG GCTCGCTGGC TGCAGACCAT TGATGTTGAT 
TCTGGTCTGC CTGTCTATGC CCCTCTAGAA GTCACAGTAA AAGAGACAAA ACTTTATTCA 
GAAACAAAAT TTTGCGAGAT AACACCGTGC ATTCTGCCAG AGCGTGCTAT TCTAAAGAGA 
ATTTGTGTAT GTGGTCCCCG ATACTGGCCC CAACAAATTG AGCTAGTCCC CGAAGAGAAA 
AATTGGTGGA GCTTTGGGGA CAAGAGTGAT CCCTTCAGTT CTGGTGTTAT ATATGTCAAA 
AGGAAAGTAG GAGCTTGTTC GTATGTTGAT GATCCAGTTG GGTGTCAGTC ACTGCTTTCG 
CGAGCAATGC ATAAGGTTTT TGGTTTAAGG ACTCTGGGAG AATCTAATTT GATTGCTAAT 
AGTCATAGAG AACTGGATTC TGATAGTGTT GATCACTTGG TCAGCACTTT CTCATCAGAT 
CCAAGCCTAA TAGCGTTTGC ACAGTTGTGC TGTGACAAAT CTTGGAATAA CAGATCTGAT 
TCTGAATTCA AGGAGTTCTG TCTGCAAGTG TTGTTTGACT GTATAAGCAA GGATAGACCA 
GCTCTTTTGC AGGTTTATTT ATCTTTGTAC ACAACCATTG GTTCGATGGC TGATCTGCTT 
GTCAAAAGCG ATAGTAACAT GTGTGATTCA CTTTCCATCT CAAGCCTCAA GGTTGCTCTT 
GCCTACAATG AGGCAGTATC AACTGGAAGA TTGGCCTCCT CAGGTGGCTT TGTACAGTCA 
ATCTTCTTAG CATCGCTGAG AAAGCGATGT GAAGAGGTTT TGAATTGCTC AACGGAGCTG 
AAGATTAATT TGAGGAATTA TTTGACCTCA GAAGCATGGC CTTATGATAA GAACTCAAAG 
TTGCAGAAAG ATATAATTAT TCTCTCATGG TATCTAAAAT GGTTCAACGT ACCATCTCCA 
TCGATCATCA AAGCAGCAGT GGAAAAGATC AAATCCAAGT CCAAAAACTC AACTTCGGCA 
ATACCTTTGT TGCGATTGTT GTTACCAAAT ACGCATATTA GTGTGATTGG TGAAATTGAT 
AGGGTCTTTT TCCCCTCGAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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EMB2771 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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