LDA cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	LDA
Entrez Gene ID	830315
Full Name	limit dextrinase
Synonyms	ATLDA,ATPU1,LIMIT DEXTRINASE,PU1,PULLULANASE,PULLULANASE 1,T19N18.90,T19N18_90,limit dextrinase
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001342767.1	NP_001330089.1	limit dextrinase
NM_120518.5	NP_196056.2	limit dextrinase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	LDA	NP_196056.2
Oryza sativa (rice)	Os04g0164900	NP_001052129.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LDA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LDA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb32155	NM_001342767.1 Latest version!	Arabidopsis thaliana limit dextrinase (LDA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb32155	NM_120518.5 Latest version!	Arabidopsis thaliana limit dextrinase (LDA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LDA ( NM_001342767.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001330089.1 Arabidopsis thaliana limit dextrinase (LDA), mRNA.

Price & Availability↑

CloneID	OAb32155
Clone ID Related Accession (Same CDS sequence)	NM_001342767.1 , NM_120518.5
Accession Version	NM_001342767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	limit dextrinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGGCACTGA CACTGACACC AACAAGTTCC GTTCATCTTC TCAGTTCCAT ATCCGTCGCG 
CGTCCTCGGA TCTTCGCCGC TGATTTTAAT CTTCGATCGC GGTGGCGACG TCGTCGGCCT 
GTTACGTCAA TTTCTAACTT CCGTCTCAGA CTTCCTTCGA AAACTTCGCT TCACTGTCTC 
TGTTCTTCGT CTTCCGCTTC ATCTCCTATG TCGCTCGAAG TCTCCAGTCC CAATTCTCAG 
TTTCTTGATT GTTTGATCTA TTCAAGAGCG TATTGGGTTA CCCAAGGCGT GATTGCATGG 
AATGTGGATG TTGGTGAAGG GTCTTGTTAC TTTTACGCGA GCAAAAGTGC TGGTTTATCA 
TTCAGCGAAG ATGGAATCGA TGGTTATGAT TTAAGAATCA AGCTTGAAGC TGAATCCGGT 
TCACTTCCTG CAGATGTAAT TGAGAAGTTT CCACATATTA GGAATTACAA ATCGTTCAAA 
GTTCCTAAGG ATTTGGATAT CAGGGATCTT GTCAAAAGCC AGTTAGCTGT TGTTTGTTTT 
GATGCGGAAG GACGGCTGAT TCAAGGAACT GGGTTGCAGT TGCCTGGCGT CCTGGATGAA 
TTGTTCTCAT ATGATGGTCC CCTAGGTGCA CATTTTACAC CAGAAGGTGT CTCTCTTCAC 
CTCTGGGCTC CCACTGCTCA AGCAGTATCT GTTTGCATCT ATAAAAACCC ACTTGACAAG 
AGTCCGATGG AAATTTGCCC ACTCAAAGAG GCTAACGGTG TGTGGAGCAC TGAAGGAGCT 
TGTAGTTGGG GAGGATGCTA TTACGTGTAT AAAGTATCAG TCTATCATCC AAGCACCATG 
AAATTGGAAA CTTGCTATGC TAATGATCCT TATGCACGGG GGCTTTCAGC AGATGGGAGG 
AAAACTTTTC TGGTTAATCT TGATTCTGAT GATCTAAAAC CTGAAGGATG GGACAACTTA 
GCAGATAAGA AACCATGCCT AAGATCCTTC TCAGATATAA GTATTTATGA GCTGCATGTG 
AGGGATTTCA GTGCCAATGA TGAGACTGTT GAGCCTGAAA ATCGTGGTGG ATATCTGGCT 
TTCACTTCAA AGGATTCTGC AGGTGTGAAA CATCTTCAGA AATTAGTTGA TGCAGGTCTT 
ACCCATCTAC ATCTTCTTCC AACATTTCAG TTTGGTGATG TTGATGATGA GAAGGAGAAT 
TGGAAGTCTG TAGACACCAG TCTGCTGGAA GGATTACGAC CTGATTCAAC TGAGGCACAA 
GCTCGTATTA CAGAAATCCA GAATGATGAT GGCTATAACT GGGGGTATAA TCCTGTGCTC 
TGGGGAGTCC CAAAAGGAAG CTATGCTAGT GATCCGACTG GTCCATGCCG CATAATTGAA 
TTTAGAAAAA TGGTTCAGGC TCTTAATTGT ACTGGTCTTA ATGTCGTCTT AGATGTTGTT 
TACAACCACT TGCATGCAAG TGGGCCACAT GACAAAGAAT CTGTTCTTGA TAAGATAGTT 
CCAGGTTACT ATTTGAGAAG GAACAGTGAT GGTTTTATTG AAAACAGTAC ATGCGTAAAC 
AACACTGCTA GCGAGCACTA TATGGTTGAT CGTCTCATAC GGGATGATCT ATTAAATTGG 
GTTGTTAACT ATAAGGTTGA TGGTTTCCGT TTTGATCTTA TGGGTCATAT AATGAAAGCT 
ACAATTGTAA ACGCAAAATC TGCAATTGGT AGCTTGAGAA AGGAAACAGA TGGAGTTGAT 
GGTTCAAGAA TTTATTTATA TGGCGAAGGA TGGAACTTTG GTGAAGTTGC TGAAAATGGA 
CGTGGAATAA ATGCGTCACA GTTCAATTTA GGCGGGACAG GAATTGGAAG TTTTAACGAC 
CGTATACGAG ATGCAACTCT TGGTGGATCT CCATTTGGTC ATCCTCTTCA ACAAGGATTC 
ATTACAGGTT TATTGTTACA GCCTAACGCT CATGACCATG GTTCAGAAGC TACACAGGAA 
CTGATGCTTT CCACTGCGAA AAATCACATC CAGACTGGTA TGGCAGCAAA TTTGAAGGAT 
TATATGCTGA CCAACCACGA AGGAAAGGAG GTGAAGGGAT CAGAGGTTTT AATGCATGAT 
GCTACACCTG TTGCATATGC TTCACTGCCA ACAGAAACTA TTAATTACGT ATCTGCTCAT 
GACAATGAAA CTCTCTTTGA CATTATCAGT TTGAAGACAC CGATGGAAAT ATCTGTTGAT 
GAAAGGTGTC GAATCAATCA TTTGGCGTCA AGTATGATTG CCCTCTCTCA GGGTATTCCA 
TTCTTCCATG CTGGGGATGA GATTCTACGC TCGAAATCAC TTGATCGAGA TTCTTACAAC 
TCCGGTGACT GGTTCAATAG GCTGGACTTC AGCTACAGTT CCAACAATTG GGGTGTTGGG 
CTTCCTCCAA AAGGGAAAAA CGAACACAAC TGGCCACTGA TCAAACCGAG GCTACAAGAT 
CCATCTTTCA AGCCTAAGAG CAGTCACATA GTTGCCACCC TCCACAATTT CTTAGATTTG 
TTACGCATCA GATACTCTTC ACCTCTCTTT CGTTTAGATA CAGCAAGGGC TATACAGGAG 
CGAGTGCGAT TTCATAACAC AGGCCCCTCA TCAATCCCTG GTGCCATTGT CATGAGCATT 
GAAGATGGTC ACAGAGGCAT CCCAAGTGTA TCTCAGATCG ATCCAATCTA CTCACTCATT 
GTGGTAATCT TCAATGCACG CCCGTCTGAG TTCTCATATC CCAGTCCCGC TCTAAAAGAC 
AGGAAGCTCG AATTACACCC GGTTCAGGTG ATGTCAGCCG ATGAAATAGT CAAGAAATCA 
GTTTACGACT CGTTTTCTGG GGGCTTCACT GTACCGGCAA GAACAACGAC GGTGTTTGTT 
GAATCGCGGA ATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001330089.1
CDS	106..3003
Translation	MALTLTPTSSVHLLSSISVARPRIFAADFNLRSRWRRRRPVTSISNFRLRLPSKTSLHCLCSSSSASSPMSLEVSSPNSQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSEDGIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEGRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPMEICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESRNG

Target ORF information:

RefSeq Version	NM_001342767.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana limit dextrinase (LDA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342767.1

ORF Insert Sequence:

ATGGCACTGA CACTGACACC AACAAGTTCC GTTCATCTTC TCAGTTCCAT ATCCGTCGCG 
CGTCCTCGGA TCTTCGCCGC TGATTTTAAT CTTCGATCGC GGTGGCGACG TCGTCGGCCT 
GTTACGTCAA TTTCTAACTT CCGTCTCAGA CTTCCTTCGA AAACTTCGCT TCACTGTCTC 
TGTTCTTCGT CTTCCGCTTC ATCTCCTATG TCGCTCGAAG TCTCCAGTCC CAATTCTCAG 
TTTCTTGATT GTTTGATCTA TTCAAGAGCG TATTGGGTTA CCCAAGGCGT GATTGCATGG 
AATGTGGATG TTGGTGAAGG GTCTTGTTAC TTTTACGCGA GCAAAAGTGC TGGTTTATCA 
TTCAGCGAAG ATGGAATCGA TGGTTATGAT TTAAGAATCA AGCTTGAAGC TGAATCCGGT 
TCACTTCCTG CAGATGTAAT TGAGAAGTTT CCACATATTA GGAATTACAA ATCGTTCAAA 
GTTCCTAAGG ATTTGGATAT CAGGGATCTT GTCAAAAGCC AGTTAGCTGT TGTTTGTTTT 
GATGCGGAAG GACGGCTGAT TCAAGGAACT GGGTTGCAGT TGCCTGGCGT CCTGGATGAA 
TTGTTCTCAT ATGATGGTCC CCTAGGTGCA CATTTTACAC CAGAAGGTGT CTCTCTTCAC 
CTCTGGGCTC CCACTGCTCA AGCAGTATCT GTTTGCATCT ATAAAAACCC ACTTGACAAG 
AGTCCGATGG AAATTTGCCC ACTCAAAGAG GCTAACGGTG TGTGGAGCAC TGAAGGAGCT 
TGTAGTTGGG GAGGATGCTA TTACGTGTAT AAAGTATCAG TCTATCATCC AAGCACCATG 
AAATTGGAAA CTTGCTATGC TAATGATCCT TATGCACGGG GGCTTTCAGC AGATGGGAGG 
AAAACTTTTC TGGTTAATCT TGATTCTGAT GATCTAAAAC CTGAAGGATG GGACAACTTA 
GCAGATAAGA AACCATGCCT AAGATCCTTC TCAGATATAA GTATTTATGA GCTGCATGTG 
AGGGATTTCA GTGCCAATGA TGAGACTGTT GAGCCTGAAA ATCGTGGTGG ATATCTGGCT 
TTCACTTCAA AGGATTCTGC AGGTGTGAAA CATCTTCAGA AATTAGTTGA TGCAGGTCTT 
ACCCATCTAC ATCTTCTTCC AACATTTCAG TTTGGTGATG TTGATGATGA GAAGGAGAAT 
TGGAAGTCTG TAGACACCAG TCTGCTGGAA GGATTACGAC CTGATTCAAC TGAGGCACAA 
GCTCGTATTA CAGAAATCCA GAATGATGAT GGCTATAACT GGGGGTATAA TCCTGTGCTC 
TGGGGAGTCC CAAAAGGAAG CTATGCTAGT GATCCGACTG GTCCATGCCG CATAATTGAA 
TTTAGAAAAA TGGTTCAGGC TCTTAATTGT ACTGGTCTTA ATGTCGTCTT AGATGTTGTT 
TACAACCACT TGCATGCAAG TGGGCCACAT GACAAAGAAT CTGTTCTTGA TAAGATAGTT 
CCAGGTTACT ATTTGAGAAG GAACAGTGAT GGTTTTATTG AAAACAGTAC ATGCGTAAAC 
AACACTGCTA GCGAGCACTA TATGGTTGAT CGTCTCATAC GGGATGATCT ATTAAATTGG 
GTTGTTAACT ATAAGGTTGA TGGTTTCCGT TTTGATCTTA TGGGTCATAT AATGAAAGCT 
ACAATTGTAA ACGCAAAATC TGCAATTGGT AGCTTGAGAA AGGAAACAGA TGGAGTTGAT 
GGTTCAAGAA TTTATTTATA TGGCGAAGGA TGGAACTTTG GTGAAGTTGC TGAAAATGGA 
CGTGGAATAA ATGCGTCACA GTTCAATTTA GGCGGGACAG GAATTGGAAG TTTTAACGAC 
CGTATACGAG ATGCAACTCT TGGTGGATCT CCATTTGGTC ATCCTCTTCA ACAAGGATTC 
ATTACAGGTT TATTGTTACA GCCTAACGCT CATGACCATG GTTCAGAAGC TACACAGGAA 
CTGATGCTTT CCACTGCGAA AAATCACATC CAGACTGGTA TGGCAGCAAA TTTGAAGGAT 
TATATGCTGA CCAACCACGA AGGAAAGGAG GTGAAGGGAT CAGAGGTTTT AATGCATGAT 
GCTACACCTG TTGCATATGC TTCACTGCCA ACAGAAACTA TTAATTACGT ATCTGCTCAT 
GACAATGAAA CTCTCTTTGA CATTATCAGT TTGAAGACAC CGATGGAAAT ATCTGTTGAT 
GAAAGGTGTC GAATCAATCA TTTGGCGTCA AGTATGATTG CCCTCTCTCA GGGTATTCCA 
TTCTTCCATG CTGGGGATGA GATTCTACGC TCGAAATCAC TTGATCGAGA TTCTTACAAC 
TCCGGTGACT GGTTCAATAG GCTGGACTTC AGCTACAGTT CCAACAATTG GGGTGTTGGG 
CTTCCTCCAA AAGGGAAAAA CGAACACAAC TGGCCACTGA TCAAACCGAG GCTACAAGAT 
CCATCTTTCA AGCCTAAGAG CAGTCACATA GTTGCCACCC TCCACAATTT CTTAGATTTG 
TTACGCATCA GATACTCTTC ACCTCTCTTT CGTTTAGATA CAGCAAGGGC TATACAGGAG 
CGAGTGCGAT TTCATAACAC AGGCCCCTCA TCAATCCCTG GTGCCATTGT CATGAGCATT 
GAAGATGGTC ACAGAGGCAT CCCAAGTGTA TCTCAGATCG ATCCAATCTA CTCACTCATT 
GTGGTAATCT TCAATGCACG CCCGTCTGAG TTCTCATATC CCAGTCCCGC TCTAAAAGAC 
AGGAAGCTCG AATTACACCC GGTTCAGGTG ATGTCAGCCG ATGAAATAGT CAAGAAATCA 
GTTTACGACT CGTTTTCTGG GGGCTTCACT GTACCGGCAA GAACAACGAC GGTGTTTGTT 
GAATCGCGGA ATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LDA ( NM_120518.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_196056.2 Arabidopsis thaliana limit dextrinase (LDA), mRNA.

Price & Availability↑

CloneID	OAb32155
Clone ID Related Accession (Same CDS sequence)	NM_001342767.1 , NM_120518.5
Accession Version	NM_120518.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	limit dextrinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_120518.4.

ATGGCACTGA CACTGACACC AACAAGTTCC GTTCATCTTC TCAGTTCCAT ATCCGTCGCG 
CGTCCTCGGA TCTTCGCCGC TGATTTTAAT CTTCGATCGC GGTGGCGACG TCGTCGGCCT 
GTTACGTCAA TTTCTAACTT CCGTCTCAGA CTTCCTTCGA AAACTTCGCT TCACTGTCTC 
TGTTCTTCGT CTTCCGCTTC ATCTCCTATG TCGCTCGAAG TCTCCAGTCC CAATTCTCAG 
TTTCTTGATT GTTTGATCTA TTCAAGAGCG TATTGGGTTA CCCAAGGCGT GATTGCATGG 
AATGTGGATG TTGGTGAAGG GTCTTGTTAC TTTTACGCGA GCAAAAGTGC TGGTTTATCA 
TTCAGCGAAG ATGGAATCGA TGGTTATGAT TTAAGAATCA AGCTTGAAGC TGAATCCGGT 
TCACTTCCTG CAGATGTAAT TGAGAAGTTT CCACATATTA GGAATTACAA ATCGTTCAAA 
GTTCCTAAGG ATTTGGATAT CAGGGATCTT GTCAAAAGCC AGTTAGCTGT TGTTTGTTTT 
GATGCGGAAG GACGGCTGAT TCAAGGAACT GGGTTGCAGT TGCCTGGCGT CCTGGATGAA 
TTGTTCTCAT ATGATGGTCC CCTAGGTGCA CATTTTACAC CAGAAGGTGT CTCTCTTCAC 
CTCTGGGCTC CCACTGCTCA AGCAGTATCT GTTTGCATCT ATAAAAACCC ACTTGACAAG 
AGTCCGATGG AAATTTGCCC ACTCAAAGAG GCTAACGGTG TGTGGAGCAC TGAAGGAGCT 
TGTAGTTGGG GAGGATGCTA TTACGTGTAT AAAGTATCAG TCTATCATCC AAGCACCATG 
AAATTGGAAA CTTGCTATGC TAATGATCCT TATGCACGGG GGCTTTCAGC AGATGGGAGG 
AAAACTTTTC TGGTTAATCT TGATTCTGAT GATCTAAAAC CTGAAGGATG GGACAACTTA 
GCAGATAAGA AACCATGCCT AAGATCCTTC TCAGATATAA GTATTTATGA GCTGCATGTG 
AGGGATTTCA GTGCCAATGA TGAGACTGTT GAGCCTGAAA ATCGTGGTGG ATATCTGGCT 
TTCACTTCAA AGGATTCTGC AGGTGTGAAA CATCTTCAGA AATTAGTTGA TGCAGGTCTT 
ACCCATCTAC ATCTTCTTCC AACATTTCAG TTTGGTGATG TTGATGATGA GAAGGAGAAT 
TGGAAGTCTG TAGACACCAG TCTGCTGGAA GGATTACGAC CTGATTCAAC TGAGGCACAA 
GCTCGTATTA CAGAAATCCA GAATGATGAT GGCTATAACT GGGGGTATAA TCCTGTGCTC 
TGGGGAGTCC CAAAAGGAAG CTATGCTAGT GATCCGACTG GTCCATGCCG CATAATTGAA 
TTTAGAAAAA TGGTTCAGGC TCTTAATTGT ACTGGTCTTA ATGTCGTCTT AGATGTTGTT 
TACAACCACT TGCATGCAAG TGGGCCACAT GACAAAGAAT CTGTTCTTGA TAAGATAGTT 
CCAGGTTACT ATTTGAGAAG GAACAGTGAT GGTTTTATTG AAAACAGTAC ATGCGTAAAC 
AACACTGCTA GCGAGCACTA TATGGTTGAT CGTCTCATAC GGGATGATCT ATTAAATTGG 
GTTGTTAACT ATAAGGTTGA TGGTTTCCGT TTTGATCTTA TGGGTCATAT AATGAAAGCT 
ACAATTGTAA ACGCAAAATC TGCAATTGGT AGCTTGAGAA AGGAAACAGA TGGAGTTGAT 
GGTTCAAGAA TTTATTTATA TGGCGAAGGA TGGAACTTTG GTGAAGTTGC TGAAAATGGA 
CGTGGAATAA ATGCGTCACA GTTCAATTTA GGCGGGACAG GAATTGGAAG TTTTAACGAC 
CGTATACGAG ATGCAACTCT TGGTGGATCT CCATTTGGTC ATCCTCTTCA ACAAGGATTC 
ATTACAGGTT TATTGTTACA GCCTAACGCT CATGACCATG GTTCAGAAGC TACACAGGAA 
CTGATGCTTT CCACTGCGAA AAATCACATC CAGACTGGTA TGGCAGCAAA TTTGAAGGAT 
TATATGCTGA CCAACCACGA AGGAAAGGAG GTGAAGGGAT CAGAGGTTTT AATGCATGAT 
GCTACACCTG TTGCATATGC TTCACTGCCA ACAGAAACTA TTAATTACGT ATCTGCTCAT 
GACAATGAAA CTCTCTTTGA CATTATCAGT TTGAAGACAC CGATGGAAAT ATCTGTTGAT 
GAAAGGTGTC GAATCAATCA TTTGGCGTCA AGTATGATTG CCCTCTCTCA GGGTATTCCA 
TTCTTCCATG CTGGGGATGA GATTCTACGC TCGAAATCAC TTGATCGAGA TTCTTACAAC 
TCCGGTGACT GGTTCAATAG GCTGGACTTC AGCTACAGTT CCAACAATTG GGGTGTTGGG 
CTTCCTCCAA AAGGGAAAAA CGAACACAAC TGGCCACTGA TCAAACCGAG GCTACAAGAT 
CCATCTTTCA AGCCTAAGAG CAGTCACATA GTTGCCACCC TCCACAATTT CTTAGATTTG 
TTACGCATCA GATACTCTTC ACCTCTCTTT CGTTTAGATA CAGCAAGGGC TATACAGGAG 
CGAGTGCGAT TTCATAACAC AGGCCCCTCA TCAATCCCTG GTGCCATTGT CATGAGCATT 
GAAGATGGTC ACAGAGGCAT CCCAAGTGTA TCTCAGATCG ATCCAATCTA CTCACTCATT 
GTGGTAATCT TCAATGCACG CCCGTCTGAG TTCTCATATC CCAGTCCCGC TCTAAAAGAC 
AGGAAGCTCG AATTACACCC GGTTCAGGTG ATGTCAGCCG ATGAAATAGT CAAGAAATCA 
GTTTACGACT CGTTTTCTGG GGGCTTCACT GTACCGGCAA GAACAACGAC GGTGTTTGTT 
GAATCGCGGA ATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_196056.2
CDS	119..3016
Translation	MALTLTPTSSVHLLSSISVARPRIFAADFNLRSRWRRRRPVTSISNFRLRLPSKTSLHCLCSSSSASSPMSLEVSSPNSQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSEDGIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEGRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPMEICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESRNG

Target ORF information:

RefSeq Version	NM_120518.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana limit dextrinase (LDA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_120518.5

ORF Insert Sequence:

ATGGCACTGA CACTGACACC AACAAGTTCC GTTCATCTTC TCAGTTCCAT ATCCGTCGCG 
CGTCCTCGGA TCTTCGCCGC TGATTTTAAT CTTCGATCGC GGTGGCGACG TCGTCGGCCT 
GTTACGTCAA TTTCTAACTT CCGTCTCAGA CTTCCTTCGA AAACTTCGCT TCACTGTCTC 
TGTTCTTCGT CTTCCGCTTC ATCTCCTATG TCGCTCGAAG TCTCCAGTCC CAATTCTCAG 
TTTCTTGATT GTTTGATCTA TTCAAGAGCG TATTGGGTTA CCCAAGGCGT GATTGCATGG 
AATGTGGATG TTGGTGAAGG GTCTTGTTAC TTTTACGCGA GCAAAAGTGC TGGTTTATCA 
TTCAGCGAAG ATGGAATCGA TGGTTATGAT TTAAGAATCA AGCTTGAAGC TGAATCCGGT 
TCACTTCCTG CAGATGTAAT TGAGAAGTTT CCACATATTA GGAATTACAA ATCGTTCAAA 
GTTCCTAAGG ATTTGGATAT CAGGGATCTT GTCAAAAGCC AGTTAGCTGT TGTTTGTTTT 
GATGCGGAAG GACGGCTGAT TCAAGGAACT GGGTTGCAGT TGCCTGGCGT CCTGGATGAA 
TTGTTCTCAT ATGATGGTCC CCTAGGTGCA CATTTTACAC CAGAAGGTGT CTCTCTTCAC 
CTCTGGGCTC CCACTGCTCA AGCAGTATCT GTTTGCATCT ATAAAAACCC ACTTGACAAG 
AGTCCGATGG AAATTTGCCC ACTCAAAGAG GCTAACGGTG TGTGGAGCAC TGAAGGAGCT 
TGTAGTTGGG GAGGATGCTA TTACGTGTAT AAAGTATCAG TCTATCATCC AAGCACCATG 
AAATTGGAAA CTTGCTATGC TAATGATCCT TATGCACGGG GGCTTTCAGC AGATGGGAGG 
AAAACTTTTC TGGTTAATCT TGATTCTGAT GATCTAAAAC CTGAAGGATG GGACAACTTA 
GCAGATAAGA AACCATGCCT AAGATCCTTC TCAGATATAA GTATTTATGA GCTGCATGTG 
AGGGATTTCA GTGCCAATGA TGAGACTGTT GAGCCTGAAA ATCGTGGTGG ATATCTGGCT 
TTCACTTCAA AGGATTCTGC AGGTGTGAAA CATCTTCAGA AATTAGTTGA TGCAGGTCTT 
ACCCATCTAC ATCTTCTTCC AACATTTCAG TTTGGTGATG TTGATGATGA GAAGGAGAAT 
TGGAAGTCTG TAGACACCAG TCTGCTGGAA GGATTACGAC CTGATTCAAC TGAGGCACAA 
GCTCGTATTA CAGAAATCCA GAATGATGAT GGCTATAACT GGGGGTATAA TCCTGTGCTC 
TGGGGAGTCC CAAAAGGAAG CTATGCTAGT GATCCGACTG GTCCATGCCG CATAATTGAA 
TTTAGAAAAA TGGTTCAGGC TCTTAATTGT ACTGGTCTTA ATGTCGTCTT AGATGTTGTT 
TACAACCACT TGCATGCAAG TGGGCCACAT GACAAAGAAT CTGTTCTTGA TAAGATAGTT 
CCAGGTTACT ATTTGAGAAG GAACAGTGAT GGTTTTATTG AAAACAGTAC ATGCGTAAAC 
AACACTGCTA GCGAGCACTA TATGGTTGAT CGTCTCATAC GGGATGATCT ATTAAATTGG 
GTTGTTAACT ATAAGGTTGA TGGTTTCCGT TTTGATCTTA TGGGTCATAT AATGAAAGCT 
ACAATTGTAA ACGCAAAATC TGCAATTGGT AGCTTGAGAA AGGAAACAGA TGGAGTTGAT 
GGTTCAAGAA TTTATTTATA TGGCGAAGGA TGGAACTTTG GTGAAGTTGC TGAAAATGGA 
CGTGGAATAA ATGCGTCACA GTTCAATTTA GGCGGGACAG GAATTGGAAG TTTTAACGAC 
CGTATACGAG ATGCAACTCT TGGTGGATCT CCATTTGGTC ATCCTCTTCA ACAAGGATTC 
ATTACAGGTT TATTGTTACA GCCTAACGCT CATGACCATG GTTCAGAAGC TACACAGGAA 
CTGATGCTTT CCACTGCGAA AAATCACATC CAGACTGGTA TGGCAGCAAA TTTGAAGGAT 
TATATGCTGA CCAACCACGA AGGAAAGGAG GTGAAGGGAT CAGAGGTTTT AATGCATGAT 
GCTACACCTG TTGCATATGC TTCACTGCCA ACAGAAACTA TTAATTACGT ATCTGCTCAT 
GACAATGAAA CTCTCTTTGA CATTATCAGT TTGAAGACAC CGATGGAAAT ATCTGTTGAT 
GAAAGGTGTC GAATCAATCA TTTGGCGTCA AGTATGATTG CCCTCTCTCA GGGTATTCCA 
TTCTTCCATG CTGGGGATGA GATTCTACGC TCGAAATCAC TTGATCGAGA TTCTTACAAC 
TCCGGTGACT GGTTCAATAG GCTGGACTTC AGCTACAGTT CCAACAATTG GGGTGTTGGG 
CTTCCTCCAA AAGGGAAAAA CGAACACAAC TGGCCACTGA TCAAACCGAG GCTACAAGAT 
CCATCTTTCA AGCCTAAGAG CAGTCACATA GTTGCCACCC TCCACAATTT CTTAGATTTG 
TTACGCATCA GATACTCTTC ACCTCTCTTT CGTTTAGATA CAGCAAGGGC TATACAGGAG 
CGAGTGCGAT TTCATAACAC AGGCCCCTCA TCAATCCCTG GTGCCATTGT CATGAGCATT 
GAAGATGGTC ACAGAGGCAT CCCAAGTGTA TCTCAGATCG ATCCAATCTA CTCACTCATT 
GTGGTAATCT TCAATGCACG CCCGTCTGAG TTCTCATATC CCAGTCCCGC TCTAAAAGAC 
AGGAAGCTCG AATTACACCC GGTTCAGGTG ATGTCAGCCG ATGAAATAGT CAAGAAATCA 
GTTTACGACT CGTTTTCTGG GGGCTTCACT GTACCGGCAA GAACAACGAC GGTGTTTGTT 
GAATCGCGGA ATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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LDA cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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