AT4G40000 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G40000
Entrez Gene ID	830161
Full Name	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Synonyms	T5J17.170,T5J17_170
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_120164.5	NP_195710.3	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
NM_001342563.1	NP_001329785.1	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NSUN2	NP_001026175.1
Oryza sativa (rice)	Os08g0484400	NP_001062081.1
Canis lupus familiaris (dog)	NSUN2	XP_535800.2
Schizosaccharomyces pombe (fission yeast)	SPAC17D4.04	NP_594695.3
Neurospora crassa	NCU05301	XP_962072.1
Arabidopsis thaliana (thale cress)	AT4G40000	NP_195710.3
Oryza sativa (rice)	Os09g0471900	NP_001063445.1
Pan troglodytes (chimpanzee)	NSUN2	XP_517620.2
Mus musculus (house mouse)	Nsun2	NP_663329.3
Drosophila melanogaster (fruit fly)	Nsun2	NP_652007.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002504	XP_563662.3
Caenorhabditis elegans (roundworm)	Y48G8AL.5	NP_490830.2
Eremothecium gossypii	AGOS_AFR179C	NP_985726.2
Homo sapiens (human)	NSUN2	NP_060225.4
Saccharomyces cerevisiae (baker's yeast)	NCL1	NP_009529.1
Kluyveromyces lactis	KLLA0F12562g	XP_455648.1
	NSUN2	XP_002804365.1
Bos taurus (cattle)	NSUN2	XP_002696495.1
Rattus norvegicus (Norway rat)	Nsun2	NP_001101873.1
Danio rerio (zebrafish)	nsun2	NP_956005.1
Schizosaccharomyces pombe (fission yeast)	SPAC23C4.17	NP_593189.2
Magnaporthe oryzae (rice blast fungus)	MGG_13217	XP_003711942.1
Arabidopsis thaliana (thale cress)	AT2G22400	NP_850024.1
Xenopus tropicalis (tropical clawed frog)	nsun2	NP_001015962.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT4G40000 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G40000 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb17582	NM_120164.5 Latest version!	Arabidopsis thaliana S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (AT4G40000), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb40182	NM_001342563.1 Latest version!	Arabidopsis thaliana S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (AT4G40000), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G40000 ( NM_120164.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_195710.3 Arabidopsis thaliana S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (AT4G40000), mRNA.

Price & Availability↑

CloneID	OAb17582
Clone ID Related Accession (Same CDS sequence)	NM_120164.5
Accession Version	NM_120164.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_120164.4.

ATGGGAAGAG GGAGACGACA CAGAGGTTCA ACTTCGAAGA GAAATCAAAT CGAAAATAGC 
AACGACGACA ATGTTCGAAA ACCCCTCAAA TCCGAAGCTT CCTTTGATTC CAACAACTTG 
CAAAAGCCCA TCTGCTCAAA TCTTCATTTT GAGGAGTATT ACAAGGAACA AAGAATAGTG 
AAAGCTCAAG AGTGGGATTC GTTTATGGAG TTTCTTCGTA AGCCATTACC TGCTGCTTTC 
AGAGTTTATT CCAATGGTCA ATTTTGTGAT GAGATTCGAC TGAAATTGGA GAATGATTTC 
ATGATATCTC TTCAGGCTGA GGCTATAGAG AGTGGTGAAT TGGAGGCTAT CAAGCCTCTG 
CCTTGGTATC CTAAGAATCT TGCTTGGCAT TCTAATTTTT CACGCAAGGA GATTAGGAAA 
AACCAGACAC TTGAGAGGTT TCATGAGTTT CTTAAGTTAG AAACTGAAGC TGGGAACATG 
ACCAGGCAAG AATCAGTTAG CATGGTACCT CCTCTCTTCC TTGATGTACA TCCAGATCAT 
TTTGTTCTCG ACATGTGTGC TGCACCTGGT TCCAAGACAT TTCAGCTGCT TGAAATAATA 
CATGAATCAT CAGAACCAGG ATCTCTACCT AATGGAATGG TGGTGGCGAA TGATGTTGAT 
TATAAAAGAT CTAATCTTCT TATTCACCAA ACGAAAAGAA CATGTACGAC CAATTTGATG 
GTCACTAACA ACGAAGGTCA ACATTTCCCC AGTTGCAACA CCAAGAGAAC CTTGTCTGTA 
GCATCTGAGA CTAATCCTCA TCCCATCGAT CAGCTTCTTT TTGATCGTGT CTTATGTGAT 
GTCCCGTGTA GTGGTGATGG TACTCTGCGC AAGGCTCCAG ACATATGGCG AAGATGGAAT 
TCTGGATCTG GCAATGGACT TCATAGTCTA CAGGTTGTGC TTGCCATGAG AGGTTTATCT 
CTGCTAAAAG TTGGTGGGAG AATGGTATAC TCAACCTGCT CAATGAACCC AATTGAAGAT 
GAAGCTGTTG TTGCTGAAAT TCTGAGGAGA TGTGGATGCT CTGTTGAGCT TGTAGATGTC 
TCAGATAAGC TTCCAGAACT TATTAGAAGA CCAGGTCTTA CGAAATGGAA GGTGCATGAC 
AGAGGTGGCT GGTATAGATC TTACAAAGAT GTTCCAAAGT CACAGAGAGA CGGAGTTCTT 
AGGAGCATGT TTCCTTCTGG TAAAAGTGAT AAAGACTCAT CGGGTGGCAA AAACAGCTAT 
GAAGAAATGG CTTCTATCTC TTCTGATGAA TCAGCCGAAG AAGTCTGTGA TCTTCCCCTT 
GAACTTTGTA TGAGGATACT GCCTCATGAT CAAAACACTG GGGGATTTTT CATTGCAGTC 
CTTCACAAAG TTTCACCTTT ACCAGACTTT CAGGAGAAAG TAACTCAGAA AAGGAATTTG 
TCTACTAGAA ACAACATCAA CTCATCTGAG AAGTCTTCTT CGTATGAAGC AGTATCTGAC 
ACTGTGGTTA CTAAACCTGA AGAGGGTACA GAGGAAATAG TTTTAGAAGC AACAGTTTCA 
GAGAATGGCT TTGAGCCGCC GGAGATGGAA AGCACCTGGA ATGAAGGTAT CGTAGAATTG 
GCTCAAAAAC TTGATGATAT AGAAGGCAAG AGAGAAGTAC CATCAATGCA AGGGAAGTGG 
AAAGGCCTTT ACCCTGTTGT TTTCTTGAGA GATCAAACAG GGATCAACGG GATCAAGACA 
TTTTACGGCA TTAAAGATGA ATCGTTTCCG CTGTATGGTC ATCTCGTGAC TAGAAACAGC 
GACATAAGCA GCCACGGAAA CGTGAAAAGG ATTTACTATG TTTCGAAAGC AGTTAAGAAT 
GTTCTTGAGC TTAATTTTGC AGTTGGGAAG CCGCTCAAGA TCTCCTCTGT TGGCCTCAAG 
ATGTTTGAGA AACAATCTGC AAAAGAATGT GAAGCCAACT GTTGCTCATT CCGAATAACG 
TCAGAGGGAT TGCCTGTGAT CCTTCCGTAC ATGACCAAGC AAATACTTTA TGCAACAATG 
GCGGATTTCA AGAATCTCCT GCAACACAAA TCAATCAAGT TTCTAGATTT CATACATCCA 
CAGATTGGTG AGAAAGCAGC AGAACTAGCC TTGGGATCTT GCGTTATGGT TCTCGTTGAT 
GATTCTCAGC TCGGGTCAGA ACAATTCAAA GTGAATTCAT CAACAATAGC CATTGGATGC 
TGGAAGGGCA AGGCTAGCTT AACCGTTATG GTCACAACAG TCGATTGTCA CCAACTTATC 
CAGAGGCTTT CTGATAGAAG TAAAGAAAAA GAAAAAGCTG AGAACTGTTT CAAGACTGCT 
GAGACAGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_195710.3
CDS	114..2465
Translation	MGRGRRHRGSTSKRNQIENSNDDNVRKPLKSEASFDSNNLQKPICSNLHFEEYYKEQRIVKAQEWDSFMEFLRKPLPAAFRVYSNGQFCDEIRLKLENDFMISLQAEAIESGELEAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLETEAGNMTRQESVSMVPPLFLDVHPDHFVLDMCAAPGSKTFQLLEIIHESSEPGSLPNGMVVANDVDYKRSNLLIHQTKRTCTTNLMVTNNEGQHFPSCNTKRTLSVASETNPHPIDQLLFDRVLCDVPCSGDGTLRKAPDIWRRWNSGSGNGLHSLQVVLAMRGLSLLKVGGRMVYSTCSMNPIEDEAVVAEILRRCGCSVELVDVSDKLPELIRRPGLTKWKVHDRGGWYRSYKDVPKSQRDGVLRSMFPSGKSDKDSSGGKNSYEEMASISSDESAEEVCDLPLELCMRILPHDQNTGGFFIAVLHKVSPLPDFQEKVTQKRNLSTRNNINSSEKSSSYEAVSDTVVTKPEEGTEEIVLEATVSENGFEPPEMESTWNEGIVELAQKLDDIEGKREVPSMQGKWKGLYPVVFLRDQTGINGIKTFYGIKDESFPLYGHLVTRNSDISSHGNVKRIYYVSKAVKNVLELNFAVGKPLKISSVGLKMFEKQSAKECEANCCSFRITSEGLPVILPYMTKQILYATMADFKNLLQHKSIKFLDFIHPQIGEKAAELALGSCVMVLVDDSQLGSEQFKVNSSTIAIGCWKGKASLTVMVTTVDCHQLIQRLSDRSKEKEKAENCFKTAETA

Target ORF information:

RefSeq Version	NM_120164.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (AT4G40000), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_120164.5

ORF Insert Sequence:

ATGGGAAGAG GGAGACGACA CAGAGGTTCA ACTTCGAAGA GAAATCAAAT CGAAAATAGC 
AACGACGACA ATGTTCGAAA ACCCCTCAAA TCCGAAGCTT CCTTTGATTC CAACAACTTG 
CAAAAGCCCA TCTGCTCAAA TCTTCATTTT GAGGAGTATT ACAAGGAACA AAGAATAGTG 
AAAGCTCAAG AGTGGGATTC GTTTATGGAG TTTCTTCGTA AGCCATTACC TGCTGCTTTC 
AGAGTTTATT CCAATGGTCA ATTTTGTGAT GAGATTCGAC TGAAATTGGA GAATGATTTC 
ATGATATCTC TTCAGGCTGA GGCTATAGAG AGTGGTGAAT TGGAGGCTAT CAAGCCTCTG 
CCTTGGTATC CTAAGAATCT TGCTTGGCAT TCTAATTTTT CACGCAAGGA GATTAGGAAA 
AACCAGACAC TTGAGAGGTT TCATGAGTTT CTTAAGTTAG AAACTGAAGC TGGGAACATG 
ACCAGGCAAG AATCAGTTAG CATGGTACCT CCTCTCTTCC TTGATGTACA TCCAGATCAT 
TTTGTTCTCG ACATGTGTGC TGCACCTGGT TCCAAGACAT TTCAGCTGCT TGAAATAATA 
CATGAATCAT CAGAACCAGG ATCTCTACCT AATGGAATGG TGGTGGCGAA TGATGTTGAT 
TATAAAAGAT CTAATCTTCT TATTCACCAA ACGAAAAGAA CATGTACGAC CAATTTGATG 
GTCACTAACA ACGAAGGTCA ACATTTCCCC AGTTGCAACA CCAAGAGAAC CTTGTCTGTA 
GCATCTGAGA CTAATCCTCA TCCCATCGAT CAGCTTCTTT TTGATCGTGT CTTATGTGAT 
GTCCCGTGTA GTGGTGATGG TACTCTGCGC AAGGCTCCAG ACATATGGCG AAGATGGAAT 
TCTGGATCTG GCAATGGACT TCATAGTCTA CAGGTTGTGC TTGCCATGAG AGGTTTATCT 
CTGCTAAAAG TTGGTGGGAG AATGGTATAC TCAACCTGCT CAATGAACCC AATTGAAGAT 
GAAGCTGTTG TTGCTGAAAT TCTGAGGAGA TGTGGATGCT CTGTTGAGCT TGTAGATGTC 
TCAGATAAGC TTCCAGAACT TATTAGAAGA CCAGGTCTTA CGAAATGGAA GGTGCATGAC 
AGAGGTGGCT GGTATAGATC TTACAAAGAT GTTCCAAAGT CACAGAGAGA CGGAGTTCTT 
AGGAGCATGT TTCCTTCTGG TAAAAGTGAT AAAGACTCAT CGGGTGGCAA AAACAGCTAT 
GAAGAAATGG CTTCTATCTC TTCTGATGAA TCAGCCGAAG AAGTCTGTGA TCTTCCCCTT 
GAACTTTGTA TGAGGATACT GCCTCATGAT CAAAACACTG GGGGATTTTT CATTGCAGTC 
CTTCACAAAG TTTCACCTTT ACCAGACTTT CAGGAGAAAG TAACTCAGAA AAGGAATTTG 
TCTACTAGAA ACAACATCAA CTCATCTGAG AAGTCTTCTT CGTATGAAGC AGTATCTGAC 
ACTGTGGTTA CTAAACCTGA AGAGGGTACA GAGGAAATAG TTTTAGAAGC AACAGTTTCA 
GAGAATGGCT TTGAGCCGCC GGAGATGGAA AGCACCTGGA ATGAAGGTAT CGTAGAATTG 
GCTCAAAAAC TTGATGATAT AGAAGGCAAG AGAGAAGTAC CATCAATGCA AGGGAAGTGG 
AAAGGCCTTT ACCCTGTTGT TTTCTTGAGA GATCAAACAG GGATCAACGG GATCAAGACA 
TTTTACGGCA TTAAAGATGA ATCGTTTCCG CTGTATGGTC ATCTCGTGAC TAGAAACAGC 
GACATAAGCA GCCACGGAAA CGTGAAAAGG ATTTACTATG TTTCGAAAGC AGTTAAGAAT 
GTTCTTGAGC TTAATTTTGC AGTTGGGAAG CCGCTCAAGA TCTCCTCTGT TGGCCTCAAG 
ATGTTTGAGA AACAATCTGC AAAAGAATGT GAAGCCAACT GTTGCTCATT CCGAATAACG 
TCAGAGGGAT TGCCTGTGAT CCTTCCGTAC ATGACCAAGC AAATACTTTA TGCAACAATG 
GCGGATTTCA AGAATCTCCT GCAACACAAA TCAATCAAGT TTCTAGATTT CATACATCCA 
CAGATTGGTG AGAAAGCAGC AGAACTAGCC TTGGGATCTT GCGTTATGGT TCTCGTTGAT 
GATTCTCAGC TCGGGTCAGA ACAATTCAAA GTGAATTCAT CAACAATAGC CATTGGATGC 
TGGAAGGGCA AGGCTAGCTT AACCGTTATG GTCACAACAG TCGATTGTCA CCAACTTATC 
CAGAGGCTTT CTGATAGAAG TAAAGAAAAA GAAAAAGCTG AGAACTGTTT CAAGACTGCT 
GAGACAGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G40000 ( NM_001342563.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329785.1 Arabidopsis thaliana S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (AT4G40000), partial mRNA.

Price & Availability↑

CloneID	OAb40182
Clone ID Related Accession (Same CDS sequence)	NM_001342563.1
Accession Version	NM_001342563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). COMPLETENESS: incomplete on the 3' end.

ATGGTGGTGG CGAATGATGT TGATTATAAA AGATCTAATC TTCTTATTCA CCAAACGAAA 
AGAACATGTA CGACCAATTT GATGGTCACT AACAACGAAG GTCAACATTT CCCCAGTTGC 
AACACCAAGA GAACCTTGTC TGTAGCATCT GAGACTAATC CTCATCCCAT CGATCAGCTT 
CTTTTTGATC GTGTCTTATG TGATGTCCCG TGTAGTGGTG ATGGTACTCT GCGCAAGGCT 
CCAGACATAT GGCGAAGATG GAATTCTGGA TCTGGCAATG GACTTCATAG TCTACAGGTT 
GTGCTTGCCA TGAGAGGTTT ATCTCTGCTA AAAGTTGGTG GGAGAATGGT ATACTCAACC 
TGCTCAATGA ACCCAATTGA AGATGAAGCT GTTGTTGCTG AAATTCTGAG GAGATGTGGA 
TGCTCTGTTG AGCTTGTAGA TGTCTCAGAT AAGCTTCCAG AACTTATTAG AAGACCAGGT 
CTTACGAAAT GGAAGGTGCA TGACAGAGGT GGCTGGTATA GATCTTACAA AGATGTTCCA 
AAGTCACAGA GAGACGGAGT TCTTAGGAGC ATGTTTCCTT CTGGTAAAAG TGATAAAGAC 
TCATCGGGTG GCAAAAACAG CTATGAAGAA ATGGCTTCTA TCTCTTCTGA TGAATCAGCC 
GAAGAAGTCT GTGATCTTCC CCTTGAACTT TGTATGAGGA TACTGCCTCA TGATCAAAAC 
ACTGGGGGAT TTTTCATTGC AGTCCTTCAC AAAGTTTCAC CTTTACCAGA CTTTCAGGAG 
AAAGTAACTC AGAAAAGGAA TTTGTCTACT AGAAACAACA TCAACTCATC TGAGAAGTCT 
TCTTCGTATG AAGCAGTATC TGACACTGTG GTTACTAAAC CTGAAGAGGG TACAGAGGAA 
ATAGTTTTAG AAGCAACAGT TTCAGAGAAT GGCTTTGAGC CGCCGGAGAT GGAAAGCACC 
TGGAATGAAG GTATCGTAGA ATTGGCTCAA AAACTTGATG ATATAGAAGG CAAGAGAGAA 
GTACCATCAA TGCAAGGGAA GTGGAAAGGC CTTTACCCTG TTGTTTTCTT GAGAGATCAA 
ACAGGGATCA ACGGGATCAA GACATTTTAC GGCATTAAAG ATGAATCGTT TCCGCTGTAT 
GGTCATCTCG TGACTAGAAA CAGCGACATA AGCAGCCACG GAAACGTGAA AAGGATTTAC 
TATGTTTCGA AAGCAGTTAA GAATGTTCTT GAGCTTAATT TTGCAGTTGG GAAGCCGCTC 
AAGATCTCCT CTGTTGGCCT CAAGATGTTT GAGAAACAAT CTGCAAAAGA ATGTGAAGCC 
AACTGTTGCT CATTCCGAAT AACGTCAGAG GGATTGCCTG TGATCCTTCC GTACATGACC 
AAGCAAATAC TTTATGCAAC AATGGCGGAT TTCAAGAATC TCCTGCAACA CAAATCAATC 
AAGTTTCTAG ATTTCATACA TCCACAGATT GGTGAGAAAG CAGCAGAACT AGCCTTGGGA 
TCTTGCGTTA TGGTTCTCGT TGATGATTCT CAGCTCGGGT CAGAACAATT CAAAGTGAAT 
TCATCAACAA TAGCCATTGG ATGCTGGAAG GGCAAGGCTA GCTTAACCGT TATGGTCACA 
ACAGTCGATT GTCACCAACT TATCCAGAGG CTTTCTGATA GAAGTAAAGA AAAAGAAAAA 
GCTGAGAACT GTTTCAAGAC TGCTGAGACA GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329785.1
CDS	131..1846
Translation	MVVANDVDYKRSNLLIHQTKRTCTTNLMVTNNEGQHFPSCNTKRTLSVASETNPHPIDQLLFDRVLCDVPCSGDGTLRKAPDIWRRWNSGSGNGLHSLQVVLAMRGLSLLKVGGRMVYSTCSMNPIEDEAVVAEILRRCGCSVELVDVSDKLPELIRRPGLTKWKVHDRGGWYRSYKDVPKSQRDGVLRSMFPSGKSDKDSSGGKNSYEEMASISSDESAEEVCDLPLELCMRILPHDQNTGGFFIAVLHKVSPLPDFQEKVTQKRNLSTRNNINSSEKSSSYEAVSDTVVTKPEEGTEEIVLEATVSENGFEPPEMESTWNEGIVELAQKLDDIEGKREVPSMQGKWKGLYPVVFLRDQTGINGIKTFYGIKDESFPLYGHLVTRNSDISSHGNVKRIYYVSKAVKNVLELNFAVGKPLKISSVGLKMFEKQSAKECEANCCSFRITSEGLPVILPYMTKQILYATMADFKNLLQHKSIKFLDFIHPQIGEKAAELALGSCVMVLVDDSQLGSEQFKVNSSTIAIGCWKGKASLTVMVTTVDCHQLIQRLSDRSKEKEKAENCFKTAETA

Target ORF information:

RefSeq Version	NM_001342563.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (AT4G40000), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342563.1

ORF Insert Sequence:

ATGGTGGTGG CGAATGATGT TGATTATAAA AGATCTAATC TTCTTATTCA CCAAACGAAA 
AGAACATGTA CGACCAATTT GATGGTCACT AACAACGAAG GTCAACATTT CCCCAGTTGC 
AACACCAAGA GAACCTTGTC TGTAGCATCT GAGACTAATC CTCATCCCAT CGATCAGCTT 
CTTTTTGATC GTGTCTTATG TGATGTCCCG TGTAGTGGTG ATGGTACTCT GCGCAAGGCT 
CCAGACATAT GGCGAAGATG GAATTCTGGA TCTGGCAATG GACTTCATAG TCTACAGGTT 
GTGCTTGCCA TGAGAGGTTT ATCTCTGCTA AAAGTTGGTG GGAGAATGGT ATACTCAACC 
TGCTCAATGA ACCCAATTGA AGATGAAGCT GTTGTTGCTG AAATTCTGAG GAGATGTGGA 
TGCTCTGTTG AGCTTGTAGA TGTCTCAGAT AAGCTTCCAG AACTTATTAG AAGACCAGGT 
CTTACGAAAT GGAAGGTGCA TGACAGAGGT GGCTGGTATA GATCTTACAA AGATGTTCCA 
AAGTCACAGA GAGACGGAGT TCTTAGGAGC ATGTTTCCTT CTGGTAAAAG TGATAAAGAC 
TCATCGGGTG GCAAAAACAG CTATGAAGAA ATGGCTTCTA TCTCTTCTGA TGAATCAGCC 
GAAGAAGTCT GTGATCTTCC CCTTGAACTT TGTATGAGGA TACTGCCTCA TGATCAAAAC 
ACTGGGGGAT TTTTCATTGC AGTCCTTCAC AAAGTTTCAC CTTTACCAGA CTTTCAGGAG 
AAAGTAACTC AGAAAAGGAA TTTGTCTACT AGAAACAACA TCAACTCATC TGAGAAGTCT 
TCTTCGTATG AAGCAGTATC TGACACTGTG GTTACTAAAC CTGAAGAGGG TACAGAGGAA 
ATAGTTTTAG AAGCAACAGT TTCAGAGAAT GGCTTTGAGC CGCCGGAGAT GGAAAGCACC 
TGGAATGAAG GTATCGTAGA ATTGGCTCAA AAACTTGATG ATATAGAAGG CAAGAGAGAA 
GTACCATCAA TGCAAGGGAA GTGGAAAGGC CTTTACCCTG TTGTTTTCTT GAGAGATCAA 
ACAGGGATCA ACGGGATCAA GACATTTTAC GGCATTAAAG ATGAATCGTT TCCGCTGTAT 
GGTCATCTCG TGACTAGAAA CAGCGACATA AGCAGCCACG GAAACGTGAA AAGGATTTAC 
TATGTTTCGA AAGCAGTTAA GAATGTTCTT GAGCTTAATT TTGCAGTTGG GAAGCCGCTC 
AAGATCTCCT CTGTTGGCCT CAAGATGTTT GAGAAACAAT CTGCAAAAGA ATGTGAAGCC 
AACTGTTGCT CATTCCGAAT AACGTCAGAG GGATTGCCTG TGATCCTTCC GTACATGACC 
AAGCAAATAC TTTATGCAAC AATGGCGGAT TTCAAGAATC TCCTGCAACA CAAATCAATC 
AAGTTTCTAG ATTTCATACA TCCACAGATT GGTGAGAAAG CAGCAGAACT AGCCTTGGGA 
TCTTGCGTTA TGGTTCTCGT TGATGATTCT CAGCTCGGGT CAGAACAATT CAAAGTGAAT 
TCATCAACAA TAGCCATTGG ATGCTGGAAG GGCAAGGCTA GCTTAACCGT TATGGTCACA 
ACAGTCGATT GTCACCAACT TATCCAGAGG CTTTCTGATA GAAGTAAAGA AAAAGAAAAA 
GCTGAGAACT GTTTCAAGAC TGCTGAGACA GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AT4G40000 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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