KAK cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	KAK
Entrez Gene ID	830017
Full Name	HECT ubiquitin protein ligase family protein KAK
Synonyms	AT4G38610,F20M13.160,F20M13_160,KAKTUS,UBIQUITIN-PROTEIN LIGASE 3,UBIQUITIN_PROTEIN LIGASE,UPL3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_120021.2	NP_195572.2	HECT ubiquitin protein ligase family protein KAK
NM_179236.3	NP_849567.2	HECT ubiquitin protein ligase family protein KAK
NM_001342508.1	NP_001329354.1	HECT ubiquitin protein ligase family protein KAK

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001296	XP_321851.5
	TRIP12	XP_002799130.1
Mus musculus (house mouse)	Trip12	NP_598736.4
Xenopus tropicalis (tropical clawed frog)	trip12	NP_001136384.1
Homo sapiens (human)	TRIP12	NP_004229.1
Canis lupus familiaris (dog)	TRIP12	XP_005635860.1
Gallus gallus (chicken)	TRIP12	XP_004937121.1
Pan troglodytes (chimpanzee)	TRIP12	XP_001138950.1
Bos taurus (cattle)	TRIP12	NP_001178132.1
Arabidopsis thaliana (thale cress)	KAK	NP_849567.2
Rattus norvegicus (Norway rat)	Trip12	NP_001026829.1
Danio rerio (zebrafish)	trip12	XP_005159284.1
Oryza sativa (rice)	Os02g0101700	NP_001045591.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a recessive mutation in the KAKTUS (KAK) gene that suppressed dwarfism and the collapsed xylem character, the cause of decreased hydraulic conductivity in the esk1-5 mutant.
Title: Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis.

Mutation in the gl3 locus negatively affects UPL3 expression, whereas overexpression of GL3 moderately upregulates it, suggesting the presence of a regulatory loop involving GL3 and UPL3.
Title: Ubiquitin protein ligase 3 mediates the proteasomal degradation of GLABROUS 3 and ENHANCER OF GLABROUS 3, regulators of trichome development and flavonoid biosynthesis in Arabidopsis.

The following KAK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KAK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb17450	NM_120021.2 Latest version!	Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OAb17449	NM_001342508.1 Latest version!	Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OAb17449	NM_179236.3 Latest version!	Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KAK ( NM_120021.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_195572.2 Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.

Price & Availability↑

CloneID	OAb17450
Clone ID Related Accession (Same CDS sequence)	NM_120021.2
Accession Version	NM_120021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	HECT ubiquitin protein ligase family protein KAK
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On May 13, 2003 this sequence version replaced NM_120021.1.

ATGGACACCT CCACCGACTC TTCTGGATTT CGCCGAGGCG GACGTGGTAA CAGGGGAAAC 
AACAACGATA ATTCTGACAA AGGTAAGGAG AAGGAACATG ACGTTAGGAT TAGGGAGCGT 
GAAAGAGAAA GAGACCGAGC CAGAGAACAA CTCAACATGG ATGCTGCCGC CGCCGCTGCT 
AGGAGCGCTG ACGAGGATGA CGACAATGAC AGTGAGGATG GCAACGGCGG TTTCATGCAT 
CCTAACATGA GCTCTGCGAG CAGTGCTTTA CAAGGCTTGC TCAGGAAGCT CGGTGCTGGA 
TTGGATGACT TGCTTCCTTC TTCCGGTATC GGCTCTGCTT CTTCCTCCCA CTTGAATGGA 
AGGATGAAGA AGATTCTCTC TGGCTTGCGC GCTGAAGGAG AAGAGGGAAA ACAGGTCGAG 
GCTTTAACCC AGCTTTGTGA GATGTTATCC ATTGGGACCG AAGACTCGCT TAGCACCTTC 
TCTGTTGATT CCTTCGTCCC AGTTCTTGTC GGTCTACTTA ACCATGAAAG CAATCCCGAC 
ATTATGCTTC TTGCTGCCAG GGCTCTTACC CATCTATGTG ATGTCTTGCC GTCTTCTTGT 
GCTGCTGTTG TACATTACGG GGCAGTTTCA TGCTTGGTGG CCAGATTGCT AACCATAGAA 
TACATGGACT TGGCGGAACA GTCTCTGCAA GCTCTCAAAA AGATATCTCA GGAGCACCCA 
ACTGCCTGTT TGCGAGCTGG TGCTCTTATG GCTGTGCTCT CGTATCTGGA TTTCTTCTCC 
ACTGGTGTTC AGCGCGTAGC ACTATCTACT GCTGCCAACA TGTGCAAGAA ACTACCTTCT 
GATGCATCTG ATTATGTTAT GGAAGCTGTA CCTTTGCTGA CAAACCTACT TCAGTATCAT 
GATTCGAAGG TTTTGGAATA TGCTTCTATC TGTCTGACTC GAATTGCTGA AGCATTTGCA 
CCGTATCCCG AGAAATTAGA TGAATTATGT AACCATGGCC TGGTGACGCA AGCTGCGTCT 
CTTATTTCCA CGAGCAATTC AGGAGGTGGG CAAGCATCTC TTAGTGTGTC AACATACACG 
GGGTTAATCC GATTACTTTC TACCTGTGCG AGCGGGTCAC CTCTTGGATT CAGGACATTA 
CTTCTTCTTG GTATTAGTAG CATTCTTAAG GATATTCTGT TGGGTTCTGG GGTCTCTGCT 
AATGCATCTG TATCCCCAGC ACTGAGCCGG CCTGCAGATC AGATTTATGA GATAGTCAAC 
CTAGCGAATG AGCTCCTCCC TCCATTGCCA GAAGGAGTTA TCTCTCTTCC TACTAGCACA 
AACGCTCTTG TGAAAGGTTC ATGCCAAAAG AAATCTAGTC CAAGTACTTC AGGAAAACAA 
GAAGATATTC TAAAAATTTC ACCAAGAGAA AAATTACTTG GTGATCAACC TGAACTTCTG 
CAGCAGTTTG GATTGGATCT TCTTCCAGTT TTAGTGCAGA TCTATGGTTC TAGTGTCAAT 
GGTACGATTC GCCATAAATG TCTCTCAGTC ATTGGAAAGT TGATGTATTT CAGCAGTTCA 
GAAATGATTC AATCTCTAAT TGGTGACACA AATATTTCGA GCTTCTTGGC TGGTGTCTTG 
GCATGGAAAG ACCCACAGGT CTTGGTTCCT GCTCTACAAG TTGCAGAGAT TTTGATGGAA 
AAGCTTCCTG AAACATTCTC GAAAGTGTTT GTGAGGGAAG GGGTAGTCCA TGCTGTAGAT 
CAACTTGTCT TGGTTGGTAA ACCATCCCAT GCCTCACCTA CTGATAAGGA CAATGACTGT 
GTACCCGGAT CTGCACGATC TAGGCGTTAT AGACGGCGCA GTAGTAATGC CAATTCCGAT 
GGAAACCAGT CGGAAGAGCC TAAGAATCCT GCGTCCCTTA CCATAGGGGC AAACCATAAT 
TCCCTTGATA CTCCTACAGC TAGCTTCATG CTAAGGGAAA CAGTTAGTTC CTGCGCCAAA 
GCATTCAAAG ACAAGTACTT CCCGTCTGAT GGTGGGGATG TTGATGTTGG AGTTACAGAT 
GATCTTTTAC ATCTGAAGAA TCTTTGCACG AAGCTAACTG CTGGTATAGA TGATCATAAA 
GTGAAAGGAA AGGGAAAATC TAAAGCCTCT GGGCCATTCC TTGGCGATTT CTCTGCTAGC 
AAGGAAGAGT ACTTGATTGG TGTCATTTCT GAGATACTTG GCGAGATAAG TAAAGGGGAT 
GGTGTCTCAA CTTTTGAGTT TATTGGCAGT GGTGTGGTTG CAGCATTGCT TAACTATTTT 
TCTTGTGGAT ACTTTTCCAA AGAGAAGATC TCCGAACTTA ATTTGCCCAA ACTTCGCCAG 
GAGGGACTCA GAAGGTTTAA AGCTTTTCTA GAAGTCGCTC TTCCTTTTGA TGGTAATGAG 
GGAAAGGTCC CTCCTATGAC AGTTTTGATT CAGAAACTTC AAAATGCTTT ATCGTCACTG 
GAGCGCTTTC CTGTTGTCCT TAGCCATCCC TCAAGGTCAC TAAGTGGAAG TGCTCGGCTC 
TCCTCGGGTT TGAGTGCTTT GGCACATCCT TTAAAGTTGC GATTATGCCG AGCATCTGGA 
GAGAAAACAC TACGTGATTA CTCCTCCAAT ATTGTACTTA TAGATCCATT GGCAAGCTTA 
GCAGCAGTGG AGGAATTTCT GTGGCCCCGA GTTCAACGGA GTGAATCTGC TCTGAAGCCG 
GCAGCGCCTA TTGGCAATAC AGAGCCAGGC ACGTTACCTA GCGGTGCTGG TGTTTCATCA 
CCATCTTCGT CAACTCCAGC TTCAACCACT CGTCGTCATT CTTCTAGATC TCGATCGGCA 
ATTAACATCG GTGATACTTC AAAGAAAGAT CCTGTGCATG AGAAAGGTAC CAGCTCATCG 
AAAGGAAAAG GTAAAGGCGT TATGAAACCG GCTCAGGCGG ATAAGGGGCC TCAAACAAGG 
AGCAATGCTC AAAAGAGAGC TGATGAGGAA TTGGAAATAT CCCCAGTCGA CATTGATGAT 
GCCTTGGTGA TTGAAGAGGA TGACATTTCT GATGATGAAG ATGATGATAA TGAAGATGTT 
TTGGATGACA GTCTTCCCAT GTGCACGCCT GATAAAGTCC ATGATGTGAA ATTGGCGGAC 
TCAGTGGATG ATGATGGTCT AGCAACCAGC GGCCGACAAA TGAATCCAGC TTCTGGAGGC 
ACTAGTGGAG CCGCAGCAGC AAGGGCATCT GATTCTATTG ATACTGGCAT TGGGAATTCC 
TATGGTTCTA GAGGTGCACT CTCCTTTGCT GCTGCAGCGA TGGCTGGGCT TGGAGCTGCC 
AGTGGTAGAG GTATCAGGGG AAGTAGGGAC TTGCATGGAC GTACCCTAAA TCGAAGTTCA 
GATGAGCCCT CTAAGTTGAT ATTTACTGCG GCAGGAAAAC AACTTAGTAG GCATTTGACG 
ATTTATCAGG CTGTACAGCG ACAACTTATG CTAGATGAAG ATGATGATGA CAGGTTTGGT 
GGCAGTGATC TAGTCTCAAG TGATGGAAGC AGATTCAATG ATATTTACAC CATCATGTAC 
CAGAGGCCAG ACAGCCAAGT GAATAGGTTG TCTGTTGGTG GAGCAAGTTC TACCACACCG 
TCAAAATCCA CGAAATCTGC TACTACCAAT TCCAGTGTAG AATCTCAGTC ACATAGGGCA 
TCTCTTTTGG ATAGTATCTT ACAAGGGGAG CTTCCATGCG ACCTTGAGAA GTCGAATTCT 
ACATATAATG TTCTGGCACT GTTACGTGTA TTAGAGGGTT TAAATCAGCT TTGCCCTCGT 
TTAAGAGCCC AAACTCTTTC CGATCGTTTT GCAGAGGGTA AAATTACAAG TCTAGATGAT 
CTGAGTACAA CTGCTGCTAA GGTTCCTCTT GATGAATTTG TCAATAGCAA ACTTACACCC 
AAATTGGCTC GACAAATCCA GGATGCGCTT GCTTTGTGCA GTGGAAGTCT TCCCTCTTGG 
TGCTACCAGT TGACTAGAGC ATGCCCATTT TTGTTTCCGT TTCAAACCCG GAGACAGTAT 
TTCTACTCGA CTGCTTTTGG GTTGTCTCGT GCATTGAATC GTTTGCAGCA GCAGCAAGGT 
GCTGACGGCA GTGGGTCTAC AAATGAACGA GAGATGAGAA TAGGGAGATT GCAGCGCCAG 
AAAGTCCGTG TATCCCGAAA TAGGATATTA GATTCTGCTG CAAAAGTTAT GGAGATGTAT 
TCTAGCCAGA AAGCTGTGCT TGAAGTAGAA TATTTTGGTG AAGTTGGTAC TGGTCTAGGC 
CCTACCCTTG AGTTTTACAC ACTTCTAAGC CATGATCTGC AAAAGGCTTC CCTAGGGATG 
TGGAGATCAA GTTCTGGTGA CAAGGTATCT ATGCAAATTG GTAGAGATGA GATTGAAGAC 
GGAAAACCAT CTGCAGCTAA CAGAGATATA GTTCTGGCAC CACTTGGATT GTTTCCTCGG 
CCTTGGCCCT CAACAGCTGA CATATCTGAA GGTGGTCAGT TTCATAAAGT CATTGAATAT 
TTCCGCCTTT TAGGGCGTGT GATGGCCAAA GCACTTCAAG ATGGACGGCT ATTGGACGTC 
CCATTGAGTA CAGCGTTTTA TAAACTTATT CTTGGTCAAG AGCTTGATTT GCATGATATT 
GTATTATTTG ACGCTGAACT TGGCAAGACC TTGCAAGAGC TGCGTGTTGT TGTTGCCCGC 
AAGCACTATC TGGAGGGAGT AGGTGGTGAC AATAGCAGCA CGATTTCTGA TTTATGTTTA 
CGTGGATGCC GAATAGAAGA TCTCTCCTTG GAATTCACGC TACCTGGCTA TCCTGAGTAC 
ATCCTGAGAT CAGGAGATGA AATTGTTGAT ATTACTAATC TTGAGGAGTA TATATCCCTT 
GTCGTTGATG CTACTGTCAA GAGAGGAGTC ACTCGGCAGA TCGAAGCCTT CAGATCTGGA 
TTCAATCAGG TGTTTGACAT AACATCTCTA CAAATATTCA CCCCTTCTGA GCTGGACTAT 
TTGCTGTGTG GTCGTAGAGA GTTGTGGGAG GTGGAGACTC TTGCTGAACA TATCAAATTT 
GATCATGGGT ATAATGCCAA AAGTCCGGCA ATCATTAACA TCATGGGAGA ACTTACAGCA 
GATCAGCAGA GGGCTTTCTG CCAATTTGTA ACTGGAGCTC CTAGGCTTCC TCCTGGTGGC 
TTAGCTGTTC TGAACCCAAA GCTTACGATT GTGAGAAAGC ACTCATCGAC CTCAAGTGCA 
GCAGCCAACG GAGCAGGGGC TTCGGAGACA GCAGATGATG ATTTGCCCAG TGTCATGACT 
TGCGCAAACT ACCTTAAACT CCCTCCTTAT TCTACAAAGG AAATCATGTA CAAGAAACTG 
CTCTACGCCA TCAACGAAGG GCAAGGATCG TTCGACCTCT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_195572.2
CDS	180..5564
Translation	MDTSTDSSGFRRGGRGNRGNNNDNSDKGKEKEHDVRIRERERERDRAREQLNMDAAAAAARSADEDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSGLRAEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCDVLPSSCAAVVHYGAVSCLVARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTSNSGGGQASLSVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDILLGSGVSANASVSPALSRPADQIYEIVNLANELLPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQEDILKISPREKLLGDQPELLQQFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSEMIQSLIGDTNISSFLAGVLAWKDPQVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPSHASPTDKDNDCVPGSARSRRYRRRSSNANSDGNQSEEPKNPASLTIGANHNSLDTPTASFMLRETVSSCAKAFKDKYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGIDDHKVKGKGKSKASGPFLGDFSASKEEYLIGVISEILGEISKGDGVSTFEFIGSGVVAALLNYFSCGYFSKEKISELNLPKLRQEGLRRFKAFLEVALPFDGNEGKVPPMTVLIQKLQNALSSLERFPVVLSHPSRSLSGSARLSSGLSALAHPLKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSESALKPAAPIGNTEPGTLPSGAGVSSPSSSTPASTTRRHSSRSRSAINIGDTSKKDPVHEKGTSSSKGKGKGVMKPAQADKGPQTRSNAQKRADEELEISPVDIDDALVIEEDDISDDEDDDNEDVLDDSLPMCTPDKVHDVKLADSVDDDGLATSGRQMNPASGGTSGAAAARASDSIDTGIGNSYGSRGALSFAAAAMAGLGAASGRGIRGSRDLHGRTLNRSSDEPSKLIFTAAGKQLSRHLTIYQAVQRQLMLDEDDDDRFGGSDLVSSDGSRFNDIYTIMYQRPDSQVNRLSVGGASSTTPSKSTKSATTNSSVESQSHRASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLCPRLRAQTLSDRFAEGKITSLDDLSTTAAKVPLDEFVNSKLTPKLARQIQDALALCSGSLPSWCYQLTRACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKASLGMWRSSSGDKVSMQIGRDEIEDGKPSAANRDIVLAPLGLFPRPWPSTADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTLQELRVVVARKHYLEGVGGDNSSTISDLCLRGCRIEDLSLEFTLPGYPEYILRSGDEIVDITNLEEYISLVVDATVKRGVTRQIEAFRSGFNQVFDITSLQIFTPSELDYLLCGRRELWEVETLAEHIKFDHGYNAKSPAIINIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS

Target ORF information:

RefSeq Version	NM_120021.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_120021.2

ORF Insert Sequence:

ATGGACACCT CCACCGACTC TTCTGGATTT CGCCGAGGCG GACGTGGTAA CAGGGGAAAC 
AACAACGATA ATTCTGACAA AGGTAAGGAG AAGGAACATG ACGTTAGGAT TAGGGAGCGT 
GAAAGAGAAA GAGACCGAGC CAGAGAACAA CTCAACATGG ATGCTGCCGC CGCCGCTGCT 
AGGAGCGCTG ACGAGGATGA CGACAATGAC AGTGAGGATG GCAACGGCGG TTTCATGCAT 
CCTAACATGA GCTCTGCGAG CAGTGCTTTA CAAGGCTTGC TCAGGAAGCT CGGTGCTGGA 
TTGGATGACT TGCTTCCTTC TTCCGGTATC GGCTCTGCTT CTTCCTCCCA CTTGAATGGA 
AGGATGAAGA AGATTCTCTC TGGCTTGCGC GCTGAAGGAG AAGAGGGAAA ACAGGTCGAG 
GCTTTAACCC AGCTTTGTGA GATGTTATCC ATTGGGACCG AAGACTCGCT TAGCACCTTC 
TCTGTTGATT CCTTCGTCCC AGTTCTTGTC GGTCTACTTA ACCATGAAAG CAATCCCGAC 
ATTATGCTTC TTGCTGCCAG GGCTCTTACC CATCTATGTG ATGTCTTGCC GTCTTCTTGT 
GCTGCTGTTG TACATTACGG GGCAGTTTCA TGCTTGGTGG CCAGATTGCT AACCATAGAA 
TACATGGACT TGGCGGAACA GTCTCTGCAA GCTCTCAAAA AGATATCTCA GGAGCACCCA 
ACTGCCTGTT TGCGAGCTGG TGCTCTTATG GCTGTGCTCT CGTATCTGGA TTTCTTCTCC 
ACTGGTGTTC AGCGCGTAGC ACTATCTACT GCTGCCAACA TGTGCAAGAA ACTACCTTCT 
GATGCATCTG ATTATGTTAT GGAAGCTGTA CCTTTGCTGA CAAACCTACT TCAGTATCAT 
GATTCGAAGG TTTTGGAATA TGCTTCTATC TGTCTGACTC GAATTGCTGA AGCATTTGCA 
CCGTATCCCG AGAAATTAGA TGAATTATGT AACCATGGCC TGGTGACGCA AGCTGCGTCT 
CTTATTTCCA CGAGCAATTC AGGAGGTGGG CAAGCATCTC TTAGTGTGTC AACATACACG 
GGGTTAATCC GATTACTTTC TACCTGTGCG AGCGGGTCAC CTCTTGGATT CAGGACATTA 
CTTCTTCTTG GTATTAGTAG CATTCTTAAG GATATTCTGT TGGGTTCTGG GGTCTCTGCT 
AATGCATCTG TATCCCCAGC ACTGAGCCGG CCTGCAGATC AGATTTATGA GATAGTCAAC 
CTAGCGAATG AGCTCCTCCC TCCATTGCCA GAAGGAGTTA TCTCTCTTCC TACTAGCACA 
AACGCTCTTG TGAAAGGTTC ATGCCAAAAG AAATCTAGTC CAAGTACTTC AGGAAAACAA 
GAAGATATTC TAAAAATTTC ACCAAGAGAA AAATTACTTG GTGATCAACC TGAACTTCTG 
CAGCAGTTTG GATTGGATCT TCTTCCAGTT TTAGTGCAGA TCTATGGTTC TAGTGTCAAT 
GGTACGATTC GCCATAAATG TCTCTCAGTC ATTGGAAAGT TGATGTATTT CAGCAGTTCA 
GAAATGATTC AATCTCTAAT TGGTGACACA AATATTTCGA GCTTCTTGGC TGGTGTCTTG 
GCATGGAAAG ACCCACAGGT CTTGGTTCCT GCTCTACAAG TTGCAGAGAT TTTGATGGAA 
AAGCTTCCTG AAACATTCTC GAAAGTGTTT GTGAGGGAAG GGGTAGTCCA TGCTGTAGAT 
CAACTTGTCT TGGTTGGTAA ACCATCCCAT GCCTCACCTA CTGATAAGGA CAATGACTGT 
GTACCCGGAT CTGCACGATC TAGGCGTTAT AGACGGCGCA GTAGTAATGC CAATTCCGAT 
GGAAACCAGT CGGAAGAGCC TAAGAATCCT GCGTCCCTTA CCATAGGGGC AAACCATAAT 
TCCCTTGATA CTCCTACAGC TAGCTTCATG CTAAGGGAAA CAGTTAGTTC CTGCGCCAAA 
GCATTCAAAG ACAAGTACTT CCCGTCTGAT GGTGGGGATG TTGATGTTGG AGTTACAGAT 
GATCTTTTAC ATCTGAAGAA TCTTTGCACG AAGCTAACTG CTGGTATAGA TGATCATAAA 
GTGAAAGGAA AGGGAAAATC TAAAGCCTCT GGGCCATTCC TTGGCGATTT CTCTGCTAGC 
AAGGAAGAGT ACTTGATTGG TGTCATTTCT GAGATACTTG GCGAGATAAG TAAAGGGGAT 
GGTGTCTCAA CTTTTGAGTT TATTGGCAGT GGTGTGGTTG CAGCATTGCT TAACTATTTT 
TCTTGTGGAT ACTTTTCCAA AGAGAAGATC TCCGAACTTA ATTTGCCCAA ACTTCGCCAG 
GAGGGACTCA GAAGGTTTAA AGCTTTTCTA GAAGTCGCTC TTCCTTTTGA TGGTAATGAG 
GGAAAGGTCC CTCCTATGAC AGTTTTGATT CAGAAACTTC AAAATGCTTT ATCGTCACTG 
GAGCGCTTTC CTGTTGTCCT TAGCCATCCC TCAAGGTCAC TAAGTGGAAG TGCTCGGCTC 
TCCTCGGGTT TGAGTGCTTT GGCACATCCT TTAAAGTTGC GATTATGCCG AGCATCTGGA 
GAGAAAACAC TACGTGATTA CTCCTCCAAT ATTGTACTTA TAGATCCATT GGCAAGCTTA 
GCAGCAGTGG AGGAATTTCT GTGGCCCCGA GTTCAACGGA GTGAATCTGC TCTGAAGCCG 
GCAGCGCCTA TTGGCAATAC AGAGCCAGGC ACGTTACCTA GCGGTGCTGG TGTTTCATCA 
CCATCTTCGT CAACTCCAGC TTCAACCACT CGTCGTCATT CTTCTAGATC TCGATCGGCA 
ATTAACATCG GTGATACTTC AAAGAAAGAT CCTGTGCATG AGAAAGGTAC CAGCTCATCG 
AAAGGAAAAG GTAAAGGCGT TATGAAACCG GCTCAGGCGG ATAAGGGGCC TCAAACAAGG 
AGCAATGCTC AAAAGAGAGC TGATGAGGAA TTGGAAATAT CCCCAGTCGA CATTGATGAT 
GCCTTGGTGA TTGAAGAGGA TGACATTTCT GATGATGAAG ATGATGATAA TGAAGATGTT 
TTGGATGACA GTCTTCCCAT GTGCACGCCT GATAAAGTCC ATGATGTGAA ATTGGCGGAC 
TCAGTGGATG ATGATGGTCT AGCAACCAGC GGCCGACAAA TGAATCCAGC TTCTGGAGGC 
ACTAGTGGAG CCGCAGCAGC AAGGGCATCT GATTCTATTG ATACTGGCAT TGGGAATTCC 
TATGGTTCTA GAGGTGCACT CTCCTTTGCT GCTGCAGCGA TGGCTGGGCT TGGAGCTGCC 
AGTGGTAGAG GTATCAGGGG AAGTAGGGAC TTGCATGGAC GTACCCTAAA TCGAAGTTCA 
GATGAGCCCT CTAAGTTGAT ATTTACTGCG GCAGGAAAAC AACTTAGTAG GCATTTGACG 
ATTTATCAGG CTGTACAGCG ACAACTTATG CTAGATGAAG ATGATGATGA CAGGTTTGGT 
GGCAGTGATC TAGTCTCAAG TGATGGAAGC AGATTCAATG ATATTTACAC CATCATGTAC 
CAGAGGCCAG ACAGCCAAGT GAATAGGTTG TCTGTTGGTG GAGCAAGTTC TACCACACCG 
TCAAAATCCA CGAAATCTGC TACTACCAAT TCCAGTGTAG AATCTCAGTC ACATAGGGCA 
TCTCTTTTGG ATAGTATCTT ACAAGGGGAG CTTCCATGCG ACCTTGAGAA GTCGAATTCT 
ACATATAATG TTCTGGCACT GTTACGTGTA TTAGAGGGTT TAAATCAGCT TTGCCCTCGT 
TTAAGAGCCC AAACTCTTTC CGATCGTTTT GCAGAGGGTA AAATTACAAG TCTAGATGAT 
CTGAGTACAA CTGCTGCTAA GGTTCCTCTT GATGAATTTG TCAATAGCAA ACTTACACCC 
AAATTGGCTC GACAAATCCA GGATGCGCTT GCTTTGTGCA GTGGAAGTCT TCCCTCTTGG 
TGCTACCAGT TGACTAGAGC ATGCCCATTT TTGTTTCCGT TTCAAACCCG GAGACAGTAT 
TTCTACTCGA CTGCTTTTGG GTTGTCTCGT GCATTGAATC GTTTGCAGCA GCAGCAAGGT 
GCTGACGGCA GTGGGTCTAC AAATGAACGA GAGATGAGAA TAGGGAGATT GCAGCGCCAG 
AAAGTCCGTG TATCCCGAAA TAGGATATTA GATTCTGCTG CAAAAGTTAT GGAGATGTAT 
TCTAGCCAGA AAGCTGTGCT TGAAGTAGAA TATTTTGGTG AAGTTGGTAC TGGTCTAGGC 
CCTACCCTTG AGTTTTACAC ACTTCTAAGC CATGATCTGC AAAAGGCTTC CCTAGGGATG 
TGGAGATCAA GTTCTGGTGA CAAGGTATCT ATGCAAATTG GTAGAGATGA GATTGAAGAC 
GGAAAACCAT CTGCAGCTAA CAGAGATATA GTTCTGGCAC CACTTGGATT GTTTCCTCGG 
CCTTGGCCCT CAACAGCTGA CATATCTGAA GGTGGTCAGT TTCATAAAGT CATTGAATAT 
TTCCGCCTTT TAGGGCGTGT GATGGCCAAA GCACTTCAAG ATGGACGGCT ATTGGACGTC 
CCATTGAGTA CAGCGTTTTA TAAACTTATT CTTGGTCAAG AGCTTGATTT GCATGATATT 
GTATTATTTG ACGCTGAACT TGGCAAGACC TTGCAAGAGC TGCGTGTTGT TGTTGCCCGC 
AAGCACTATC TGGAGGGAGT AGGTGGTGAC AATAGCAGCA CGATTTCTGA TTTATGTTTA 
CGTGGATGCC GAATAGAAGA TCTCTCCTTG GAATTCACGC TACCTGGCTA TCCTGAGTAC 
ATCCTGAGAT CAGGAGATGA AATTGTTGAT ATTACTAATC TTGAGGAGTA TATATCCCTT 
GTCGTTGATG CTACTGTCAA GAGAGGAGTC ACTCGGCAGA TCGAAGCCTT CAGATCTGGA 
TTCAATCAGG TGTTTGACAT AACATCTCTA CAAATATTCA CCCCTTCTGA GCTGGACTAT 
TTGCTGTGTG GTCGTAGAGA GTTGTGGGAG GTGGAGACTC TTGCTGAACA TATCAAATTT 
GATCATGGGT ATAATGCCAA AAGTCCGGCA ATCATTAACA TCATGGGAGA ACTTACAGCA 
GATCAGCAGA GGGCTTTCTG CCAATTTGTA ACTGGAGCTC CTAGGCTTCC TCCTGGTGGC 
TTAGCTGTTC TGAACCCAAA GCTTACGATT GTGAGAAAGC ACTCATCGAC CTCAAGTGCA 
GCAGCCAACG GAGCAGGGGC TTCGGAGACA GCAGATGATG ATTTGCCCAG TGTCATGACT 
TGCGCAAACT ACCTTAAACT CCCTCCTTAT TCTACAAAGG AAATCATGTA CAAGAAACTG 
CTCTACGCCA TCAACGAAGG GCAAGGATCG TTCGACCTCT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KAK ( NM_179236.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849567.2 Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.

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CloneID	OAb17449
Clone ID Related Accession (Same CDS sequence)	NM_179236.3 , NM_001342508.1
Accession Version	NM_179236.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	HECT ubiquitin protein ligase family protein KAK
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_179236.2.

ATGGAAACTC GGAGCCGCAA GCGGGCGGAG GCGACCTCAG CTGCCCCATC TTCTTCTTCT 
TCTTCTCCTC CTCCTCCTCC CTCTGCCTCT GGTCCCACCA CCCGCAGCAA ACGCGCTCGT 
CTTTCTTCTT CTTCTTCTTC CTCACTTGCC CCCACTCCTC CTTCTTCCTC CACCACCACC 
CGCTCTCGTT CTTCTCGCTC TGCCGCCGCC GCTGCTCCCA TGGACACCTC CACCGACTCT 
TCTGGATTTC GCCGAGGCGG ACGTGGTAAC AGGGGAAACA ACAACGATAA TTCTGACAAA 
GGTAAGGAGA AGGAACATGA CGTTAGGATT AGGGAGCGTG AAAGAGAAAG AGACCGAGCC 
AGAGAACAAC TCAACATGGA TGCTGCCGCC GCCGCTGCTA GGAGCGCTGA CGAGGATGAC 
GACAATGACA GTGAGGATGG CAACGGCGGT TTCATGCATC CTAACATGAG CTCTGCGAGC 
AGTGCTTTAC AAGGCTTGCT CAGGAAGCTC GGTGCTGGAT TGGATGACTT GCTTCCTTCT 
TCCGGTATCG GCTCTGCTTC TTCCTCCCAC TTGAATGGAA GGATGAAGAA GATTCTCTCT 
GGCTTGCGCG CTGAAGGAGA AGAGGGAAAA CAGGTCGAGG CTTTAACCCA GCTTTGTGAG 
ATGTTATCCA TTGGGACCGA AGACTCGCTT AGCACCTTCT CTGTTGATTC CTTCGTCCCA 
GTTCTTGTCG GTCTACTTAA CCATGAAAGC AATCCCGACA TTATGCTTCT TGCTGCCAGG 
GCTCTTACCC ATCTATGTGA TGTCTTGCCG TCTTCTTGTG CTGCTGTTGT ACATTACGGG 
GCAGTTTCAT GCTTGGTGGC CAGATTGCTA ACCATAGAAT ACATGGACTT GGCGGAACAG 
TCTCTGCAAG CTCTCAAAAA GATATCTCAG GAGCACCCAA CTGCCTGTTT GCGAGCTGGT 
GCTCTTATGG CTGTGCTCTC GTATCTGGAT TTCTTCTCCA CTGGTGTTCA GCGCGTAGCA 
CTATCTACTG CTGCCAACAT GTGCAAGAAA CTACCTTCTG ATGCATCTGA TTATGTTATG 
GAAGCTGTAC CTTTGCTGAC AAACCTACTT CAGTATCATG ATTCGAAGGT TTTGGAATAT 
GCTTCTATCT GTCTGACTCG AATTGCTGAA GCATTTGCAC CGTATCCCGA GAAATTAGAT 
GAATTATGTA ACCATGGCCT GGTGACGCAA GCTGCGTCTC TTATTTCCAC GAGCAATTCA 
GGAGGTGGGC AAGCATCTCT TAGTGTGTCA ACATACACGG GGTTAATCCG ATTACTTTCT 
ACCTGTGCGA GCGGGTCACC TCTTGGATTC AGGACATTAC TTCTTCTTGG TATTAGTAGC 
ATTCTTAAGG ATATTCTGTT GGGTTCTGGG GTCTCTGCTA ATGCATCTGT ATCCCCAGCA 
CTGAGCCGGC CTGCAGATCA GATTTATGAG ATAGTCAACC TAGCGAATGA GCTCCTCCCT 
CCATTGCCAG AAGGAGTTAT CTCTCTTCCT ACTAGCACAA ACGCTCTTGT GAAAGGTTCA 
TGCCAAAAGA AATCTAGTCC AAGTACTTCA GGAAAACAAG AAGATATTCT AAAAATTTCA 
CCAAGAGAAA AATTACTTGG TGATCAACCT GAACTTCTGC AGCAGTTTGG ATTGGATCTT 
CTTCCAGTTT TAGTGCAGAT CTATGGTTCT AGTGTCAATG GTACGATTCG CCATAAATGT 
CTCTCAGTCA TTGGAAAGTT GATGTATTTC AGCAGTTCAG AAATGATTCA ATCTCTAATT 
GGTGACACAA ATATTTCGAG CTTCTTGGCT GGTGTCTTGG CATGGAAAGA CCCACAGGTC 
TTGGTTCCTG CTCTACAAGT TGCAGAGATT TTGATGGAAA AGCTTCCTGA AACATTCTCG 
AAAGTGTTTG TGAGGGAAGG GGTAGTCCAT GCTGTAGATC AACTTGTCTT GGTTGGTAAA 
CCATCCCATG CCTCACCTAC TGATAAGGAC AATGACTGTG TACCCGGATC TGCACGATCT 
AGGCGTTATA GACGGCGCAG TAGTAATGCC AATTCCGATG GAAACCAGTC GGAAGAGCCT 
AAGAATCCTG CGTCCCTTAC CATAGGGGCA AACCATAATT CCCTTGATAC TCCTACAGCT 
AGCTTCATGC TAAGGGAAAC AGTTAGTTCC TGCGCCAAAG CATTCAAAGA CAAGTACTTC 
CCGTCTGATG GTGGGGATGT TGATGTTGGA GTTACAGATG ATCTTTTACA TCTGAAGAAT 
CTTTGCACGA AGCTAACTGC TGGTATAGAT GATCATAAAG TGAAAGGAAA GGGAAAATCT 
AAAGCCTCTG GGCCATTCCT TGGCGATTTC TCTGCTAGCA AGGAAGAGTA CTTGATTGGT 
GTCATTTCTG AGATACTTGG CGAGATAAGT AAAGGGGATG GTGTCTCAAC TTTTGAGTTT 
ATTGGCAGTG GTGTGGTTGC AGCATTGCTT AACTATTTTT CTTGTGGATA CTTTTCCAAA 
GAGAAGATCT CCGAACTTAA TTTGCCCAAA CTTCGCCAGG AGGGACTCAG AAGGTTTAAA 
GCTTTTCTAG AAGTCGCTCT TCCTTTTGAT GGTAATGAGG GAAAGGTCCC TCCTATGACA 
GTTTTGATTC AGAAACTTCA AAATGCTTTA TCGTCACTGG AGCGCTTTCC TGTTGTCCTT 
AGCCATCCCT CAAGGTCACT AAGTGGAAGT GCTCGGCTCT CCTCGGGTTT GAGTGCTTTG 
GCACATCCTT TAAAGTTGCG ATTATGCCGA GCATCTGGAG AGAAAACACT ACGTGATTAC 
TCCTCCAATA TTGTACTTAT AGATCCATTG GCAAGCTTAG CAGCAGTGGA GGAATTTCTG 
TGGCCCCGAG TTCAACGGAG TGAATCTGCT CTGAAGCCGG CAGCGCCTAT TGGCAATACA 
GAGCCAGGCA CGTTACCTAG CGGTGCTGGT GTTTCATCAC CATCTTCGTC AACTCCAGCT 
TCAACCACTC GTCGTCATTC TTCTAGATCT CGATCGGCAA TTAACATCGG TGATACTTCA 
AAGAAAGATC CTGTGCATGA GAAAGGTACC AGCTCATCGA AAGGAAAAGG TAAAGGCGTT 
ATGAAACCGG CTCAGGCGGA TAAGGGGCCT CAAACAAGGA GCAATGCTCA AAAGAGAGCT 
GTTCTTGACA AAGATACTCA AATGAAACCA GCTAGCGGAG ACTCCAGTTC TGAGGATGAG 
GAATTGGAAA TATCCCCAGT CGACATTGAT GATGCCTTGG TGATTGAAGA GGATGACATT 
TCTGATGATG AAGATGATGA TAATGAAGAT GTTTTGGATG ACAGTCTTCC CATGTGCACG 
CCTGATAAAG TCCATGATGT GAAATTGGCG GACTCAGTGG ATGATGATGG TCTAGCAACC 
AGCGGCCGAC AAATGAATCC AGCTTCTGGA GGCACTAGTG GAGCCGCAGC AGCAAGGGCA 
TCTGATTCTA TTGATACTGG CATTGGGAAT TCCTATGGTT CTAGAGGTGC ACTCTCCTTT 
GCTGCTGCAG CGATGGCTGG GCTTGGAGCT GCCAGTGGTA GAGGTATCAG GGGAAGTAGG 
GACTTGCATG GACGTACCCT AAATCGAAGT TCAGATGAGC CCTCTAAGTT GATATTTACT 
GCGGCAGGAA AACAACTTAG TAGGCATTTG ACGATTTATC AGGCTGTACA GCGACAACTT 
ATGCTAGATG AAGATGATGA TGACAGGTTT GGTGGCAGTG ATCTAGTCTC AAGTGATGGA 
AGCAGATTCA ATGATATTTA CACCATCATG TACCAGAGGC CAGACAGCCA AGTGAATAGG 
TTGTCTGTTG GTGGAGCAAG TTCTACCACA CCGTCAAAAT CCACGAAATC TGCTACTACC 
AATTCCAGTG TAGAATCTCA GTCACATAGG GCATCTCTTT TGGATAGTAT CTTACAAGGG 
GAGCTTCCAT GCGACCTTGA GAAGTCGAAT TCTACATATA ATGTTCTGGC ACTGTTACGT 
GTATTAGAGG GTTTAAATCA GCTTTGCCCT CGTTTAAGAG CCCAAACTCT TTCCGATCGT 
TTTGCAGAGG GTAAAATTAC AAGTCTAGAT GATCTGAGTA CAACTGCTGC TAAGGTTCCT 
CTTGATGAAT TTGTCAATAG CAAACTTACA CCCAAATTGG CTCGACAAAT CCAGGATGCG 
CTTGCTTTGT GCAGTGGAAG TCTTCCCTCT TGGTGCTACC AGTTGACTAG AGCATGCCCA 
TTTTTGTTTC CGTTTCAAAC CCGGAGACAG TATTTCTACT CGACTGCTTT TGGGTTGTCT 
CGTGCATTGA ATCGTTTGCA GCAGCAGCAA GGTGCTGACG GCAGTGGGTC TACAAATGAA 
CGAGAGATGA GAATAGGGAG ATTGCAGCGC CAGAAAGTCC GTGTATCCCG AAATAGGATA 
TTAGATTCTG CTGCAAAAGT TATGGAGATG TATTCTAGCC AGAAAGCTGT GCTTGAAGTA 
GAATATTTTG GTGAAGTTGG TACTGGTCTA GGCCCTACCC TTGAGTTTTA CACACTTCTA 
AGCCATGATC TGCAAAAGGC TTCCCTAGGG ATGTGGAGAT CAAGTTCTGG TGACAAGGTA 
TCTATGCAAA TTGGTAGAGA TGAGATTGAA GACGGAAAAC CATCTGCAGC TAACAGAGAT 
ATAGTTCTGG CACCACTTGG ATTGTTTCCT CGGCCTTGGC CCTCAACAGC TGACATATCT 
GAAGGTGGTC AGTTTCATAA AGTCATTGAA TATTTCCGCC TTTTAGGGCG TGTGATGGCC 
AAAGCACTTC AAGATGGACG GCTATTGGAC GTCCCATTGA GTACAGCGTT TTATAAACTT 
ATTCTTGGTC AAGAGCTTGA TTTGCATGAT ATTGTATTAT TTGACGCTGA ACTTGGCAAG 
ACCTTGCAAG AGCTGCGTGT TGTTGTTGCC CGCAAGCACT ATCTGGAGGG AGTAGGTGGT 
GACAATAGCA GCACGATTTC TGATTTATGT TTACGTGGAT GCCGAATAGA AGATCTCTCC 
TTGGAATTCA CGCTACCTGG CTATCCTGAG TACATCCTGA GATCAGGAGA TGAAATTGTT 
GATATTACTA ATCTTGAGGA GTATATATCC CTTGTCGTTG ATGCTACTGT CAAGAGAGGA 
GTCACTCGGC AGATCGAAGC CTTCAGATCT GGATTCAATC AGGTGTTTGA CATAACATCT 
CTACAAATAT TCACCCCTTC TGAGCTGGAC TATTTGCTGT GTGGTCGTAG AGAGTTGTGG 
GAGGTGGAGA CTCTTGCTGA ACATATCAAA TTTGATCATG GGTATAATGC CAAAAGTCCG 
GCAATCATTA ACTTACTGGA GATCATGGGA GAACTTACAG CAGATCAGCA GAGGGCTTTC 
TGCCAATTTG TAACTGGAGC TCCTAGGCTT CCTCCTGGTG GCTTAGCTGT TCTGAACCCA 
AAGCTTACGA TTGTGAGAAA GCACTCATCG ACCTCAAGTG CAGCAGCCAA CGGAGCAGGG 
GCTTCGGAGA CAGCAGATGA TGATTTGCCC AGTGTCATGA CTTGCGCAAA CTACCTTAAA 
CTCCCTCCTT ATTCTACAAA GGAAATCATG TACAAGAAAC TGCTCTACGC CATCAACGAA 
GGGCAAGGAT CGTTCGACCT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849567.2
CDS	409..6075
Translation	METRSRKRAEATSAAPSSSSSSPPPPPSASGPTTRSKRARLSSSSSSSLAPTPPSSSTTTRSRSSRSAAAAAPMDTSTDSSGFRRGGRGNRGNNNDNSDKGKEKEHDVRIRERERERDRAREQLNMDAAAAAARSADEDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSGLRAEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCDVLPSSCAAVVHYGAVSCLVARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTSNSGGGQASLSVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDILLGSGVSANASVSPALSRPADQIYEIVNLANELLPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQEDILKISPREKLLGDQPELLQQFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSEMIQSLIGDTNISSFLAGVLAWKDPQVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPSHASPTDKDNDCVPGSARSRRYRRRSSNANSDGNQSEEPKNPASLTIGANHNSLDTPTASFMLRETVSSCAKAFKDKYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGIDDHKVKGKGKSKASGPFLGDFSASKEEYLIGVISEILGEISKGDGVSTFEFIGSGVVAALLNYFSCGYFSKEKISELNLPKLRQEGLRRFKAFLEVALPFDGNEGKVPPMTVLIQKLQNALSSLERFPVVLSHPSRSLSGSARLSSGLSALAHPLKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSESALKPAAPIGNTEPGTLPSGAGVSSPSSSTPASTTRRHSSRSRSAINIGDTSKKDPVHEKGTSSSKGKGKGVMKPAQADKGPQTRSNAQKRAVLDKDTQMKPASGDSSSEDEELEISPVDIDDALVIEEDDISDDEDDDNEDVLDDSLPMCTPDKVHDVKLADSVDDDGLATSGRQMNPASGGTSGAAAARASDSIDTGIGNSYGSRGALSFAAAAMAGLGAASGRGIRGSRDLHGRTLNRSSDEPSKLIFTAAGKQLSRHLTIYQAVQRQLMLDEDDDDRFGGSDLVSSDGSRFNDIYTIMYQRPDSQVNRLSVGGASSTTPSKSTKSATTNSSVESQSHRASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLCPRLRAQTLSDRFAEGKITSLDDLSTTAAKVPLDEFVNSKLTPKLARQIQDALALCSGSLPSWCYQLTRACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKASLGMWRSSSGDKVSMQIGRDEIEDGKPSAANRDIVLAPLGLFPRPWPSTADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTLQELRVVVARKHYLEGVGGDNSSTISDLCLRGCRIEDLSLEFTLPGYPEYILRSGDEIVDITNLEEYISLVVDATVKRGVTRQIEAFRSGFNQVFDITSLQIFTPSELDYLLCGRRELWEVETLAEHIKFDHGYNAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS

Target ORF information:

RefSeq Version	NM_179236.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179236.3

ORF Insert Sequence:

ATGGAAACTC GGAGCCGCAA GCGGGCGGAG GCGACCTCAG CTGCCCCATC TTCTTCTTCT 
TCTTCTCCTC CTCCTCCTCC CTCTGCCTCT GGTCCCACCA CCCGCAGCAA ACGCGCTCGT 
CTTTCTTCTT CTTCTTCTTC CTCACTTGCC CCCACTCCTC CTTCTTCCTC CACCACCACC 
CGCTCTCGTT CTTCTCGCTC TGCCGCCGCC GCTGCTCCCA TGGACACCTC CACCGACTCT 
TCTGGATTTC GCCGAGGCGG ACGTGGTAAC AGGGGAAACA ACAACGATAA TTCTGACAAA 
GGTAAGGAGA AGGAACATGA CGTTAGGATT AGGGAGCGTG AAAGAGAAAG AGACCGAGCC 
AGAGAACAAC TCAACATGGA TGCTGCCGCC GCCGCTGCTA GGAGCGCTGA CGAGGATGAC 
GACAATGACA GTGAGGATGG CAACGGCGGT TTCATGCATC CTAACATGAG CTCTGCGAGC 
AGTGCTTTAC AAGGCTTGCT CAGGAAGCTC GGTGCTGGAT TGGATGACTT GCTTCCTTCT 
TCCGGTATCG GCTCTGCTTC TTCCTCCCAC TTGAATGGAA GGATGAAGAA GATTCTCTCT 
GGCTTGCGCG CTGAAGGAGA AGAGGGAAAA CAGGTCGAGG CTTTAACCCA GCTTTGTGAG 
ATGTTATCCA TTGGGACCGA AGACTCGCTT AGCACCTTCT CTGTTGATTC CTTCGTCCCA 
GTTCTTGTCG GTCTACTTAA CCATGAAAGC AATCCCGACA TTATGCTTCT TGCTGCCAGG 
GCTCTTACCC ATCTATGTGA TGTCTTGCCG TCTTCTTGTG CTGCTGTTGT ACATTACGGG 
GCAGTTTCAT GCTTGGTGGC CAGATTGCTA ACCATAGAAT ACATGGACTT GGCGGAACAG 
TCTCTGCAAG CTCTCAAAAA GATATCTCAG GAGCACCCAA CTGCCTGTTT GCGAGCTGGT 
GCTCTTATGG CTGTGCTCTC GTATCTGGAT TTCTTCTCCA CTGGTGTTCA GCGCGTAGCA 
CTATCTACTG CTGCCAACAT GTGCAAGAAA CTACCTTCTG ATGCATCTGA TTATGTTATG 
GAAGCTGTAC CTTTGCTGAC AAACCTACTT CAGTATCATG ATTCGAAGGT TTTGGAATAT 
GCTTCTATCT GTCTGACTCG AATTGCTGAA GCATTTGCAC CGTATCCCGA GAAATTAGAT 
GAATTATGTA ACCATGGCCT GGTGACGCAA GCTGCGTCTC TTATTTCCAC GAGCAATTCA 
GGAGGTGGGC AAGCATCTCT TAGTGTGTCA ACATACACGG GGTTAATCCG ATTACTTTCT 
ACCTGTGCGA GCGGGTCACC TCTTGGATTC AGGACATTAC TTCTTCTTGG TATTAGTAGC 
ATTCTTAAGG ATATTCTGTT GGGTTCTGGG GTCTCTGCTA ATGCATCTGT ATCCCCAGCA 
CTGAGCCGGC CTGCAGATCA GATTTATGAG ATAGTCAACC TAGCGAATGA GCTCCTCCCT 
CCATTGCCAG AAGGAGTTAT CTCTCTTCCT ACTAGCACAA ACGCTCTTGT GAAAGGTTCA 
TGCCAAAAGA AATCTAGTCC AAGTACTTCA GGAAAACAAG AAGATATTCT AAAAATTTCA 
CCAAGAGAAA AATTACTTGG TGATCAACCT GAACTTCTGC AGCAGTTTGG ATTGGATCTT 
CTTCCAGTTT TAGTGCAGAT CTATGGTTCT AGTGTCAATG GTACGATTCG CCATAAATGT 
CTCTCAGTCA TTGGAAAGTT GATGTATTTC AGCAGTTCAG AAATGATTCA ATCTCTAATT 
GGTGACACAA ATATTTCGAG CTTCTTGGCT GGTGTCTTGG CATGGAAAGA CCCACAGGTC 
TTGGTTCCTG CTCTACAAGT TGCAGAGATT TTGATGGAAA AGCTTCCTGA AACATTCTCG 
AAAGTGTTTG TGAGGGAAGG GGTAGTCCAT GCTGTAGATC AACTTGTCTT GGTTGGTAAA 
CCATCCCATG CCTCACCTAC TGATAAGGAC AATGACTGTG TACCCGGATC TGCACGATCT 
AGGCGTTATA GACGGCGCAG TAGTAATGCC AATTCCGATG GAAACCAGTC GGAAGAGCCT 
AAGAATCCTG CGTCCCTTAC CATAGGGGCA AACCATAATT CCCTTGATAC TCCTACAGCT 
AGCTTCATGC TAAGGGAAAC AGTTAGTTCC TGCGCCAAAG CATTCAAAGA CAAGTACTTC 
CCGTCTGATG GTGGGGATGT TGATGTTGGA GTTACAGATG ATCTTTTACA TCTGAAGAAT 
CTTTGCACGA AGCTAACTGC TGGTATAGAT GATCATAAAG TGAAAGGAAA GGGAAAATCT 
AAAGCCTCTG GGCCATTCCT TGGCGATTTC TCTGCTAGCA AGGAAGAGTA CTTGATTGGT 
GTCATTTCTG AGATACTTGG CGAGATAAGT AAAGGGGATG GTGTCTCAAC TTTTGAGTTT 
ATTGGCAGTG GTGTGGTTGC AGCATTGCTT AACTATTTTT CTTGTGGATA CTTTTCCAAA 
GAGAAGATCT CCGAACTTAA TTTGCCCAAA CTTCGCCAGG AGGGACTCAG AAGGTTTAAA 
GCTTTTCTAG AAGTCGCTCT TCCTTTTGAT GGTAATGAGG GAAAGGTCCC TCCTATGACA 
GTTTTGATTC AGAAACTTCA AAATGCTTTA TCGTCACTGG AGCGCTTTCC TGTTGTCCTT 
AGCCATCCCT CAAGGTCACT AAGTGGAAGT GCTCGGCTCT CCTCGGGTTT GAGTGCTTTG 
GCACATCCTT TAAAGTTGCG ATTATGCCGA GCATCTGGAG AGAAAACACT ACGTGATTAC 
TCCTCCAATA TTGTACTTAT AGATCCATTG GCAAGCTTAG CAGCAGTGGA GGAATTTCTG 
TGGCCCCGAG TTCAACGGAG TGAATCTGCT CTGAAGCCGG CAGCGCCTAT TGGCAATACA 
GAGCCAGGCA CGTTACCTAG CGGTGCTGGT GTTTCATCAC CATCTTCGTC AACTCCAGCT 
TCAACCACTC GTCGTCATTC TTCTAGATCT CGATCGGCAA TTAACATCGG TGATACTTCA 
AAGAAAGATC CTGTGCATGA GAAAGGTACC AGCTCATCGA AAGGAAAAGG TAAAGGCGTT 
ATGAAACCGG CTCAGGCGGA TAAGGGGCCT CAAACAAGGA GCAATGCTCA AAAGAGAGCT 
GTTCTTGACA AAGATACTCA AATGAAACCA GCTAGCGGAG ACTCCAGTTC TGAGGATGAG 
GAATTGGAAA TATCCCCAGT CGACATTGAT GATGCCTTGG TGATTGAAGA GGATGACATT 
TCTGATGATG AAGATGATGA TAATGAAGAT GTTTTGGATG ACAGTCTTCC CATGTGCACG 
CCTGATAAAG TCCATGATGT GAAATTGGCG GACTCAGTGG ATGATGATGG TCTAGCAACC 
AGCGGCCGAC AAATGAATCC AGCTTCTGGA GGCACTAGTG GAGCCGCAGC AGCAAGGGCA 
TCTGATTCTA TTGATACTGG CATTGGGAAT TCCTATGGTT CTAGAGGTGC ACTCTCCTTT 
GCTGCTGCAG CGATGGCTGG GCTTGGAGCT GCCAGTGGTA GAGGTATCAG GGGAAGTAGG 
GACTTGCATG GACGTACCCT AAATCGAAGT TCAGATGAGC CCTCTAAGTT GATATTTACT 
GCGGCAGGAA AACAACTTAG TAGGCATTTG ACGATTTATC AGGCTGTACA GCGACAACTT 
ATGCTAGATG AAGATGATGA TGACAGGTTT GGTGGCAGTG ATCTAGTCTC AAGTGATGGA 
AGCAGATTCA ATGATATTTA CACCATCATG TACCAGAGGC CAGACAGCCA AGTGAATAGG 
TTGTCTGTTG GTGGAGCAAG TTCTACCACA CCGTCAAAAT CCACGAAATC TGCTACTACC 
AATTCCAGTG TAGAATCTCA GTCACATAGG GCATCTCTTT TGGATAGTAT CTTACAAGGG 
GAGCTTCCAT GCGACCTTGA GAAGTCGAAT TCTACATATA ATGTTCTGGC ACTGTTACGT 
GTATTAGAGG GTTTAAATCA GCTTTGCCCT CGTTTAAGAG CCCAAACTCT TTCCGATCGT 
TTTGCAGAGG GTAAAATTAC AAGTCTAGAT GATCTGAGTA CAACTGCTGC TAAGGTTCCT 
CTTGATGAAT TTGTCAATAG CAAACTTACA CCCAAATTGG CTCGACAAAT CCAGGATGCG 
CTTGCTTTGT GCAGTGGAAG TCTTCCCTCT TGGTGCTACC AGTTGACTAG AGCATGCCCA 
TTTTTGTTTC CGTTTCAAAC CCGGAGACAG TATTTCTACT CGACTGCTTT TGGGTTGTCT 
CGTGCATTGA ATCGTTTGCA GCAGCAGCAA GGTGCTGACG GCAGTGGGTC TACAAATGAA 
CGAGAGATGA GAATAGGGAG ATTGCAGCGC CAGAAAGTCC GTGTATCCCG AAATAGGATA 
TTAGATTCTG CTGCAAAAGT TATGGAGATG TATTCTAGCC AGAAAGCTGT GCTTGAAGTA 
GAATATTTTG GTGAAGTTGG TACTGGTCTA GGCCCTACCC TTGAGTTTTA CACACTTCTA 
AGCCATGATC TGCAAAAGGC TTCCCTAGGG ATGTGGAGAT CAAGTTCTGG TGACAAGGTA 
TCTATGCAAA TTGGTAGAGA TGAGATTGAA GACGGAAAAC CATCTGCAGC TAACAGAGAT 
ATAGTTCTGG CACCACTTGG ATTGTTTCCT CGGCCTTGGC CCTCAACAGC TGACATATCT 
GAAGGTGGTC AGTTTCATAA AGTCATTGAA TATTTCCGCC TTTTAGGGCG TGTGATGGCC 
AAAGCACTTC AAGATGGACG GCTATTGGAC GTCCCATTGA GTACAGCGTT TTATAAACTT 
ATTCTTGGTC AAGAGCTTGA TTTGCATGAT ATTGTATTAT TTGACGCTGA ACTTGGCAAG 
ACCTTGCAAG AGCTGCGTGT TGTTGTTGCC CGCAAGCACT ATCTGGAGGG AGTAGGTGGT 
GACAATAGCA GCACGATTTC TGATTTATGT TTACGTGGAT GCCGAATAGA AGATCTCTCC 
TTGGAATTCA CGCTACCTGG CTATCCTGAG TACATCCTGA GATCAGGAGA TGAAATTGTT 
GATATTACTA ATCTTGAGGA GTATATATCC CTTGTCGTTG ATGCTACTGT CAAGAGAGGA 
GTCACTCGGC AGATCGAAGC CTTCAGATCT GGATTCAATC AGGTGTTTGA CATAACATCT 
CTACAAATAT TCACCCCTTC TGAGCTGGAC TATTTGCTGT GTGGTCGTAG AGAGTTGTGG 
GAGGTGGAGA CTCTTGCTGA ACATATCAAA TTTGATCATG GGTATAATGC CAAAAGTCCG 
GCAATCATTA ACTTACTGGA GATCATGGGA GAACTTACAG CAGATCAGCA GAGGGCTTTC 
TGCCAATTTG TAACTGGAGC TCCTAGGCTT CCTCCTGGTG GCTTAGCTGT TCTGAACCCA 
AAGCTTACGA TTGTGAGAAA GCACTCATCG ACCTCAAGTG CAGCAGCCAA CGGAGCAGGG 
GCTTCGGAGA CAGCAGATGA TGATTTGCCC AGTGTCATGA CTTGCGCAAA CTACCTTAAA 
CTCCCTCCTT ATTCTACAAA GGAAATCATG TACAAGAAAC TGCTCTACGC CATCAACGAA 
GGGCAAGGAT CGTTCGACCT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KAK ( NM_001342508.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329354.1 Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.

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CloneID	OAb17449
Clone ID Related Accession (Same CDS sequence)	NM_179236.3 , NM_001342508.1
Accession Version	NM_001342508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	HECT ubiquitin protein ligase family protein KAK
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGAAACTC GGAGCCGCAA GCGGGCGGAG GCGACCTCAG CTGCCCCATC TTCTTCTTCT 
TCTTCTCCTC CTCCTCCTCC CTCTGCCTCT GGTCCCACCA CCCGCAGCAA ACGCGCTCGT 
CTTTCTTCTT CTTCTTCTTC CTCACTTGCC CCCACTCCTC CTTCTTCCTC CACCACCACC 
CGCTCTCGTT CTTCTCGCTC TGCCGCCGCC GCTGCTCCCA TGGACACCTC CACCGACTCT 
TCTGGATTTC GCCGAGGCGG ACGTGGTAAC AGGGGAAACA ACAACGATAA TTCTGACAAA 
GGTAAGGAGA AGGAACATGA CGTTAGGATT AGGGAGCGTG AAAGAGAAAG AGACCGAGCC 
AGAGAACAAC TCAACATGGA TGCTGCCGCC GCCGCTGCTA GGAGCGCTGA CGAGGATGAC 
GACAATGACA GTGAGGATGG CAACGGCGGT TTCATGCATC CTAACATGAG CTCTGCGAGC 
AGTGCTTTAC AAGGCTTGCT CAGGAAGCTC GGTGCTGGAT TGGATGACTT GCTTCCTTCT 
TCCGGTATCG GCTCTGCTTC TTCCTCCCAC TTGAATGGAA GGATGAAGAA GATTCTCTCT 
GGCTTGCGCG CTGAAGGAGA AGAGGGAAAA CAGGTCGAGG CTTTAACCCA GCTTTGTGAG 
ATGTTATCCA TTGGGACCGA AGACTCGCTT AGCACCTTCT CTGTTGATTC CTTCGTCCCA 
GTTCTTGTCG GTCTACTTAA CCATGAAAGC AATCCCGACA TTATGCTTCT TGCTGCCAGG 
GCTCTTACCC ATCTATGTGA TGTCTTGCCG TCTTCTTGTG CTGCTGTTGT ACATTACGGG 
GCAGTTTCAT GCTTGGTGGC CAGATTGCTA ACCATAGAAT ACATGGACTT GGCGGAACAG 
TCTCTGCAAG CTCTCAAAAA GATATCTCAG GAGCACCCAA CTGCCTGTTT GCGAGCTGGT 
GCTCTTATGG CTGTGCTCTC GTATCTGGAT TTCTTCTCCA CTGGTGTTCA GCGCGTAGCA 
CTATCTACTG CTGCCAACAT GTGCAAGAAA CTACCTTCTG ATGCATCTGA TTATGTTATG 
GAAGCTGTAC CTTTGCTGAC AAACCTACTT CAGTATCATG ATTCGAAGGT TTTGGAATAT 
GCTTCTATCT GTCTGACTCG AATTGCTGAA GCATTTGCAC CGTATCCCGA GAAATTAGAT 
GAATTATGTA ACCATGGCCT GGTGACGCAA GCTGCGTCTC TTATTTCCAC GAGCAATTCA 
GGAGGTGGGC AAGCATCTCT TAGTGTGTCA ACATACACGG GGTTAATCCG ATTACTTTCT 
ACCTGTGCGA GCGGGTCACC TCTTGGATTC AGGACATTAC TTCTTCTTGG TATTAGTAGC 
ATTCTTAAGG ATATTCTGTT GGGTTCTGGG GTCTCTGCTA ATGCATCTGT ATCCCCAGCA 
CTGAGCCGGC CTGCAGATCA GATTTATGAG ATAGTCAACC TAGCGAATGA GCTCCTCCCT 
CCATTGCCAG AAGGAGTTAT CTCTCTTCCT ACTAGCACAA ACGCTCTTGT GAAAGGTTCA 
TGCCAAAAGA AATCTAGTCC AAGTACTTCA GGAAAACAAG AAGATATTCT AAAAATTTCA 
CCAAGAGAAA AATTACTTGG TGATCAACCT GAACTTCTGC AGCAGTTTGG ATTGGATCTT 
CTTCCAGTTT TAGTGCAGAT CTATGGTTCT AGTGTCAATG GTACGATTCG CCATAAATGT 
CTCTCAGTCA TTGGAAAGTT GATGTATTTC AGCAGTTCAG AAATGATTCA ATCTCTAATT 
GGTGACACAA ATATTTCGAG CTTCTTGGCT GGTGTCTTGG CATGGAAAGA CCCACAGGTC 
TTGGTTCCTG CTCTACAAGT TGCAGAGATT TTGATGGAAA AGCTTCCTGA AACATTCTCG 
AAAGTGTTTG TGAGGGAAGG GGTAGTCCAT GCTGTAGATC AACTTGTCTT GGTTGGTAAA 
CCATCCCATG CCTCACCTAC TGATAAGGAC AATGACTGTG TACCCGGATC TGCACGATCT 
AGGCGTTATA GACGGCGCAG TAGTAATGCC AATTCCGATG GAAACCAGTC GGAAGAGCCT 
AAGAATCCTG CGTCCCTTAC CATAGGGGCA AACCATAATT CCCTTGATAC TCCTACAGCT 
AGCTTCATGC TAAGGGAAAC AGTTAGTTCC TGCGCCAAAG CATTCAAAGA CAAGTACTTC 
CCGTCTGATG GTGGGGATGT TGATGTTGGA GTTACAGATG ATCTTTTACA TCTGAAGAAT 
CTTTGCACGA AGCTAACTGC TGGTATAGAT GATCATAAAG TGAAAGGAAA GGGAAAATCT 
AAAGCCTCTG GGCCATTCCT TGGCGATTTC TCTGCTAGCA AGGAAGAGTA CTTGATTGGT 
GTCATTTCTG AGATACTTGG CGAGATAAGT AAAGGGGATG GTGTCTCAAC TTTTGAGTTT 
ATTGGCAGTG GTGTGGTTGC AGCATTGCTT AACTATTTTT CTTGTGGATA CTTTTCCAAA 
GAGAAGATCT CCGAACTTAA TTTGCCCAAA CTTCGCCAGG AGGGACTCAG AAGGTTTAAA 
GCTTTTCTAG AAGTCGCTCT TCCTTTTGAT GGTAATGAGG GAAAGGTCCC TCCTATGACA 
GTTTTGATTC AGAAACTTCA AAATGCTTTA TCGTCACTGG AGCGCTTTCC TGTTGTCCTT 
AGCCATCCCT CAAGGTCACT AAGTGGAAGT GCTCGGCTCT CCTCGGGTTT GAGTGCTTTG 
GCACATCCTT TAAAGTTGCG ATTATGCCGA GCATCTGGAG AGAAAACACT ACGTGATTAC 
TCCTCCAATA TTGTACTTAT AGATCCATTG GCAAGCTTAG CAGCAGTGGA GGAATTTCTG 
TGGCCCCGAG TTCAACGGAG TGAATCTGCT CTGAAGCCGG CAGCGCCTAT TGGCAATACA 
GAGCCAGGCA CGTTACCTAG CGGTGCTGGT GTTTCATCAC CATCTTCGTC AACTCCAGCT 
TCAACCACTC GTCGTCATTC TTCTAGATCT CGATCGGCAA TTAACATCGG TGATACTTCA 
AAGAAAGATC CTGTGCATGA GAAAGGTACC AGCTCATCGA AAGGAAAAGG TAAAGGCGTT 
ATGAAACCGG CTCAGGCGGA TAAGGGGCCT CAAACAAGGA GCAATGCTCA AAAGAGAGCT 
GTTCTTGACA AAGATACTCA AATGAAACCA GCTAGCGGAG ACTCCAGTTC TGAGGATGAG 
GAATTGGAAA TATCCCCAGT CGACATTGAT GATGCCTTGG TGATTGAAGA GGATGACATT 
TCTGATGATG AAGATGATGA TAATGAAGAT GTTTTGGATG ACAGTCTTCC CATGTGCACG 
CCTGATAAAG TCCATGATGT GAAATTGGCG GACTCAGTGG ATGATGATGG TCTAGCAACC 
AGCGGCCGAC AAATGAATCC AGCTTCTGGA GGCACTAGTG GAGCCGCAGC AGCAAGGGCA 
TCTGATTCTA TTGATACTGG CATTGGGAAT TCCTATGGTT CTAGAGGTGC ACTCTCCTTT 
GCTGCTGCAG CGATGGCTGG GCTTGGAGCT GCCAGTGGTA GAGGTATCAG GGGAAGTAGG 
GACTTGCATG GACGTACCCT AAATCGAAGT TCAGATGAGC CCTCTAAGTT GATATTTACT 
GCGGCAGGAA AACAACTTAG TAGGCATTTG ACGATTTATC AGGCTGTACA GCGACAACTT 
ATGCTAGATG AAGATGATGA TGACAGGTTT GGTGGCAGTG ATCTAGTCTC AAGTGATGGA 
AGCAGATTCA ATGATATTTA CACCATCATG TACCAGAGGC CAGACAGCCA AGTGAATAGG 
TTGTCTGTTG GTGGAGCAAG TTCTACCACA CCGTCAAAAT CCACGAAATC TGCTACTACC 
AATTCCAGTG TAGAATCTCA GTCACATAGG GCATCTCTTT TGGATAGTAT CTTACAAGGG 
GAGCTTCCAT GCGACCTTGA GAAGTCGAAT TCTACATATA ATGTTCTGGC ACTGTTACGT 
GTATTAGAGG GTTTAAATCA GCTTTGCCCT CGTTTAAGAG CCCAAACTCT TTCCGATCGT 
TTTGCAGAGG GTAAAATTAC AAGTCTAGAT GATCTGAGTA CAACTGCTGC TAAGGTTCCT 
CTTGATGAAT TTGTCAATAG CAAACTTACA CCCAAATTGG CTCGACAAAT CCAGGATGCG 
CTTGCTTTGT GCAGTGGAAG TCTTCCCTCT TGGTGCTACC AGTTGACTAG AGCATGCCCA 
TTTTTGTTTC CGTTTCAAAC CCGGAGACAG TATTTCTACT CGACTGCTTT TGGGTTGTCT 
CGTGCATTGA ATCGTTTGCA GCAGCAGCAA GGTGCTGACG GCAGTGGGTC TACAAATGAA 
CGAGAGATGA GAATAGGGAG ATTGCAGCGC CAGAAAGTCC GTGTATCCCG AAATAGGATA 
TTAGATTCTG CTGCAAAAGT TATGGAGATG TATTCTAGCC AGAAAGCTGT GCTTGAAGTA 
GAATATTTTG GTGAAGTTGG TACTGGTCTA GGCCCTACCC TTGAGTTTTA CACACTTCTA 
AGCCATGATC TGCAAAAGGC TTCCCTAGGG ATGTGGAGAT CAAGTTCTGG TGACAAGGTA 
TCTATGCAAA TTGGTAGAGA TGAGATTGAA GACGGAAAAC CATCTGCAGC TAACAGAGAT 
ATAGTTCTGG CACCACTTGG ATTGTTTCCT CGGCCTTGGC CCTCAACAGC TGACATATCT 
GAAGGTGGTC AGTTTCATAA AGTCATTGAA TATTTCCGCC TTTTAGGGCG TGTGATGGCC 
AAAGCACTTC AAGATGGACG GCTATTGGAC GTCCCATTGA GTACAGCGTT TTATAAACTT 
ATTCTTGGTC AAGAGCTTGA TTTGCATGAT ATTGTATTAT TTGACGCTGA ACTTGGCAAG 
ACCTTGCAAG AGCTGCGTGT TGTTGTTGCC CGCAAGCACT ATCTGGAGGG AGTAGGTGGT 
GACAATAGCA GCACGATTTC TGATTTATGT TTACGTGGAT GCCGAATAGA AGATCTCTCC 
TTGGAATTCA CGCTACCTGG CTATCCTGAG TACATCCTGA GATCAGGAGA TGAAATTGTT 
GATATTACTA ATCTTGAGGA GTATATATCC CTTGTCGTTG ATGCTACTGT CAAGAGAGGA 
GTCACTCGGC AGATCGAAGC CTTCAGATCT GGATTCAATC AGGTGTTTGA CATAACATCT 
CTACAAATAT TCACCCCTTC TGAGCTGGAC TATTTGCTGT GTGGTCGTAG AGAGTTGTGG 
GAGGTGGAGA CTCTTGCTGA ACATATCAAA TTTGATCATG GGTATAATGC CAAAAGTCCG 
GCAATCATTA ACTTACTGGA GATCATGGGA GAACTTACAG CAGATCAGCA GAGGGCTTTC 
TGCCAATTTG TAACTGGAGC TCCTAGGCTT CCTCCTGGTG GCTTAGCTGT TCTGAACCCA 
AAGCTTACGA TTGTGAGAAA GCACTCATCG ACCTCAAGTG CAGCAGCCAA CGGAGCAGGG 
GCTTCGGAGA CAGCAGATGA TGATTTGCCC AGTGTCATGA CTTGCGCAAA CTACCTTAAA 
CTCCCTCCTT ATTCTACAAA GGAAATCATG TACAAGAAAC TGCTCTACGC CATCAACGAA 
GGGCAAGGAT CGTTCGACCT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329354.1
CDS	409..6075
Translation	METRSRKRAEATSAAPSSSSSSPPPPPSASGPTTRSKRARLSSSSSSSLAPTPPSSSTTTRSRSSRSAAAAAPMDTSTDSSGFRRGGRGNRGNNNDNSDKGKEKEHDVRIRERERERDRAREQLNMDAAAAAARSADEDDDNDSEDGNGGFMHPNMSSASSALQGLLRKLGAGLDDLLPSSGIGSASSSHLNGRMKKILSGLRAEGEEGKQVEALTQLCEMLSIGTEDSLSTFSVDSFVPVLVGLLNHESNPDIMLLAARALTHLCDVLPSSCAAVVHYGAVSCLVARLLTIEYMDLAEQSLQALKKISQEHPTACLRAGALMAVLSYLDFFSTGVQRVALSTAANMCKKLPSDASDYVMEAVPLLTNLLQYHDSKVLEYASICLTRIAEAFAPYPEKLDELCNHGLVTQAASLISTSNSGGGQASLSVSTYTGLIRLLSTCASGSPLGFRTLLLLGISSILKDILLGSGVSANASVSPALSRPADQIYEIVNLANELLPPLPEGVISLPTSTNALVKGSCQKKSSPSTSGKQEDILKISPREKLLGDQPELLQQFGLDLLPVLVQIYGSSVNGTIRHKCLSVIGKLMYFSSSEMIQSLIGDTNISSFLAGVLAWKDPQVLVPALQVAEILMEKLPETFSKVFVREGVVHAVDQLVLVGKPSHASPTDKDNDCVPGSARSRRYRRRSSNANSDGNQSEEPKNPASLTIGANHNSLDTPTASFMLRETVSSCAKAFKDKYFPSDGGDVDVGVTDDLLHLKNLCTKLTAGIDDHKVKGKGKSKASGPFLGDFSASKEEYLIGVISEILGEISKGDGVSTFEFIGSGVVAALLNYFSCGYFSKEKISELNLPKLRQEGLRRFKAFLEVALPFDGNEGKVPPMTVLIQKLQNALSSLERFPVVLSHPSRSLSGSARLSSGLSALAHPLKLRLCRASGEKTLRDYSSNIVLIDPLASLAAVEEFLWPRVQRSESALKPAAPIGNTEPGTLPSGAGVSSPSSSTPASTTRRHSSRSRSAINIGDTSKKDPVHEKGTSSSKGKGKGVMKPAQADKGPQTRSNAQKRAVLDKDTQMKPASGDSSSEDEELEISPVDIDDALVIEEDDISDDEDDDNEDVLDDSLPMCTPDKVHDVKLADSVDDDGLATSGRQMNPASGGTSGAAAARASDSIDTGIGNSYGSRGALSFAAAAMAGLGAASGRGIRGSRDLHGRTLNRSSDEPSKLIFTAAGKQLSRHLTIYQAVQRQLMLDEDDDDRFGGSDLVSSDGSRFNDIYTIMYQRPDSQVNRLSVGGASSTTPSKSTKSATTNSSVESQSHRASLLDSILQGELPCDLEKSNSTYNVLALLRVLEGLNQLCPRLRAQTLSDRFAEGKITSLDDLSTTAAKVPLDEFVNSKLTPKLARQIQDALALCSGSLPSWCYQLTRACPFLFPFQTRRQYFYSTAFGLSRALNRLQQQQGADGSGSTNEREMRIGRLQRQKVRVSRNRILDSAAKVMEMYSSQKAVLEVEYFGEVGTGLGPTLEFYTLLSHDLQKASLGMWRSSSGDKVSMQIGRDEIEDGKPSAANRDIVLAPLGLFPRPWPSTADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTAFYKLILGQELDLHDIVLFDAELGKTLQELRVVVARKHYLEGVGGDNSSTISDLCLRGCRIEDLSLEFTLPGYPEYILRSGDEIVDITNLEEYISLVVDATVKRGVTRQIEAFRSGFNQVFDITSLQIFTPSELDYLLCGRRELWEVETLAEHIKFDHGYNAKSPAIINLLEIMGELTADQQRAFCQFVTGAPRLPPGGLAVLNPKLTIVRKHSSTSSAAANGAGASETADDDLPSVMTCANYLKLPPYSTKEIMYKKLLYAINEGQGSFDLS

Target ORF information:

RefSeq Version	NM_001342508.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana HECT ubiquitin protein ligase family protein KAK (KAK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342508.1

ORF Insert Sequence:

ATGGAAACTC GGAGCCGCAA GCGGGCGGAG GCGACCTCAG CTGCCCCATC TTCTTCTTCT 
TCTTCTCCTC CTCCTCCTCC CTCTGCCTCT GGTCCCACCA CCCGCAGCAA ACGCGCTCGT 
CTTTCTTCTT CTTCTTCTTC CTCACTTGCC CCCACTCCTC CTTCTTCCTC CACCACCACC 
CGCTCTCGTT CTTCTCGCTC TGCCGCCGCC GCTGCTCCCA TGGACACCTC CACCGACTCT 
TCTGGATTTC GCCGAGGCGG ACGTGGTAAC AGGGGAAACA ACAACGATAA TTCTGACAAA 
GGTAAGGAGA AGGAACATGA CGTTAGGATT AGGGAGCGTG AAAGAGAAAG AGACCGAGCC 
AGAGAACAAC TCAACATGGA TGCTGCCGCC GCCGCTGCTA GGAGCGCTGA CGAGGATGAC 
GACAATGACA GTGAGGATGG CAACGGCGGT TTCATGCATC CTAACATGAG CTCTGCGAGC 
AGTGCTTTAC AAGGCTTGCT CAGGAAGCTC GGTGCTGGAT TGGATGACTT GCTTCCTTCT 
TCCGGTATCG GCTCTGCTTC TTCCTCCCAC TTGAATGGAA GGATGAAGAA GATTCTCTCT 
GGCTTGCGCG CTGAAGGAGA AGAGGGAAAA CAGGTCGAGG CTTTAACCCA GCTTTGTGAG 
ATGTTATCCA TTGGGACCGA AGACTCGCTT AGCACCTTCT CTGTTGATTC CTTCGTCCCA 
GTTCTTGTCG GTCTACTTAA CCATGAAAGC AATCCCGACA TTATGCTTCT TGCTGCCAGG 
GCTCTTACCC ATCTATGTGA TGTCTTGCCG TCTTCTTGTG CTGCTGTTGT ACATTACGGG 
GCAGTTTCAT GCTTGGTGGC CAGATTGCTA ACCATAGAAT ACATGGACTT GGCGGAACAG 
TCTCTGCAAG CTCTCAAAAA GATATCTCAG GAGCACCCAA CTGCCTGTTT GCGAGCTGGT 
GCTCTTATGG CTGTGCTCTC GTATCTGGAT TTCTTCTCCA CTGGTGTTCA GCGCGTAGCA 
CTATCTACTG CTGCCAACAT GTGCAAGAAA CTACCTTCTG ATGCATCTGA TTATGTTATG 
GAAGCTGTAC CTTTGCTGAC AAACCTACTT CAGTATCATG ATTCGAAGGT TTTGGAATAT 
GCTTCTATCT GTCTGACTCG AATTGCTGAA GCATTTGCAC CGTATCCCGA GAAATTAGAT 
GAATTATGTA ACCATGGCCT GGTGACGCAA GCTGCGTCTC TTATTTCCAC GAGCAATTCA 
GGAGGTGGGC AAGCATCTCT TAGTGTGTCA ACATACACGG GGTTAATCCG ATTACTTTCT 
ACCTGTGCGA GCGGGTCACC TCTTGGATTC AGGACATTAC TTCTTCTTGG TATTAGTAGC 
ATTCTTAAGG ATATTCTGTT GGGTTCTGGG GTCTCTGCTA ATGCATCTGT ATCCCCAGCA 
CTGAGCCGGC CTGCAGATCA GATTTATGAG ATAGTCAACC TAGCGAATGA GCTCCTCCCT 
CCATTGCCAG AAGGAGTTAT CTCTCTTCCT ACTAGCACAA ACGCTCTTGT GAAAGGTTCA 
TGCCAAAAGA AATCTAGTCC AAGTACTTCA GGAAAACAAG AAGATATTCT AAAAATTTCA 
CCAAGAGAAA AATTACTTGG TGATCAACCT GAACTTCTGC AGCAGTTTGG ATTGGATCTT 
CTTCCAGTTT TAGTGCAGAT CTATGGTTCT AGTGTCAATG GTACGATTCG CCATAAATGT 
CTCTCAGTCA TTGGAAAGTT GATGTATTTC AGCAGTTCAG AAATGATTCA ATCTCTAATT 
GGTGACACAA ATATTTCGAG CTTCTTGGCT GGTGTCTTGG CATGGAAAGA CCCACAGGTC 
TTGGTTCCTG CTCTACAAGT TGCAGAGATT TTGATGGAAA AGCTTCCTGA AACATTCTCG 
AAAGTGTTTG TGAGGGAAGG GGTAGTCCAT GCTGTAGATC AACTTGTCTT GGTTGGTAAA 
CCATCCCATG CCTCACCTAC TGATAAGGAC AATGACTGTG TACCCGGATC TGCACGATCT 
AGGCGTTATA GACGGCGCAG TAGTAATGCC AATTCCGATG GAAACCAGTC GGAAGAGCCT 
AAGAATCCTG CGTCCCTTAC CATAGGGGCA AACCATAATT CCCTTGATAC TCCTACAGCT 
AGCTTCATGC TAAGGGAAAC AGTTAGTTCC TGCGCCAAAG CATTCAAAGA CAAGTACTTC 
CCGTCTGATG GTGGGGATGT TGATGTTGGA GTTACAGATG ATCTTTTACA TCTGAAGAAT 
CTTTGCACGA AGCTAACTGC TGGTATAGAT GATCATAAAG TGAAAGGAAA GGGAAAATCT 
AAAGCCTCTG GGCCATTCCT TGGCGATTTC TCTGCTAGCA AGGAAGAGTA CTTGATTGGT 
GTCATTTCTG AGATACTTGG CGAGATAAGT AAAGGGGATG GTGTCTCAAC TTTTGAGTTT 
ATTGGCAGTG GTGTGGTTGC AGCATTGCTT AACTATTTTT CTTGTGGATA CTTTTCCAAA 
GAGAAGATCT CCGAACTTAA TTTGCCCAAA CTTCGCCAGG AGGGACTCAG AAGGTTTAAA 
GCTTTTCTAG AAGTCGCTCT TCCTTTTGAT GGTAATGAGG GAAAGGTCCC TCCTATGACA 
GTTTTGATTC AGAAACTTCA AAATGCTTTA TCGTCACTGG AGCGCTTTCC TGTTGTCCTT 
AGCCATCCCT CAAGGTCACT AAGTGGAAGT GCTCGGCTCT CCTCGGGTTT GAGTGCTTTG 
GCACATCCTT TAAAGTTGCG ATTATGCCGA GCATCTGGAG AGAAAACACT ACGTGATTAC 
TCCTCCAATA TTGTACTTAT AGATCCATTG GCAAGCTTAG CAGCAGTGGA GGAATTTCTG 
TGGCCCCGAG TTCAACGGAG TGAATCTGCT CTGAAGCCGG CAGCGCCTAT TGGCAATACA 
GAGCCAGGCA CGTTACCTAG CGGTGCTGGT GTTTCATCAC CATCTTCGTC AACTCCAGCT 
TCAACCACTC GTCGTCATTC TTCTAGATCT CGATCGGCAA TTAACATCGG TGATACTTCA 
AAGAAAGATC CTGTGCATGA GAAAGGTACC AGCTCATCGA AAGGAAAAGG TAAAGGCGTT 
ATGAAACCGG CTCAGGCGGA TAAGGGGCCT CAAACAAGGA GCAATGCTCA AAAGAGAGCT 
GTTCTTGACA AAGATACTCA AATGAAACCA GCTAGCGGAG ACTCCAGTTC TGAGGATGAG 
GAATTGGAAA TATCCCCAGT CGACATTGAT GATGCCTTGG TGATTGAAGA GGATGACATT 
TCTGATGATG AAGATGATGA TAATGAAGAT GTTTTGGATG ACAGTCTTCC CATGTGCACG 
CCTGATAAAG TCCATGATGT GAAATTGGCG GACTCAGTGG ATGATGATGG TCTAGCAACC 
AGCGGCCGAC AAATGAATCC AGCTTCTGGA GGCACTAGTG GAGCCGCAGC AGCAAGGGCA 
TCTGATTCTA TTGATACTGG CATTGGGAAT TCCTATGGTT CTAGAGGTGC ACTCTCCTTT 
GCTGCTGCAG CGATGGCTGG GCTTGGAGCT GCCAGTGGTA GAGGTATCAG GGGAAGTAGG 
GACTTGCATG GACGTACCCT AAATCGAAGT TCAGATGAGC CCTCTAAGTT GATATTTACT 
GCGGCAGGAA AACAACTTAG TAGGCATTTG ACGATTTATC AGGCTGTACA GCGACAACTT 
ATGCTAGATG AAGATGATGA TGACAGGTTT GGTGGCAGTG ATCTAGTCTC AAGTGATGGA 
AGCAGATTCA ATGATATTTA CACCATCATG TACCAGAGGC CAGACAGCCA AGTGAATAGG 
TTGTCTGTTG GTGGAGCAAG TTCTACCACA CCGTCAAAAT CCACGAAATC TGCTACTACC 
AATTCCAGTG TAGAATCTCA GTCACATAGG GCATCTCTTT TGGATAGTAT CTTACAAGGG 
GAGCTTCCAT GCGACCTTGA GAAGTCGAAT TCTACATATA ATGTTCTGGC ACTGTTACGT 
GTATTAGAGG GTTTAAATCA GCTTTGCCCT CGTTTAAGAG CCCAAACTCT TTCCGATCGT 
TTTGCAGAGG GTAAAATTAC AAGTCTAGAT GATCTGAGTA CAACTGCTGC TAAGGTTCCT 
CTTGATGAAT TTGTCAATAG CAAACTTACA CCCAAATTGG CTCGACAAAT CCAGGATGCG 
CTTGCTTTGT GCAGTGGAAG TCTTCCCTCT TGGTGCTACC AGTTGACTAG AGCATGCCCA 
TTTTTGTTTC CGTTTCAAAC CCGGAGACAG TATTTCTACT CGACTGCTTT TGGGTTGTCT 
CGTGCATTGA ATCGTTTGCA GCAGCAGCAA GGTGCTGACG GCAGTGGGTC TACAAATGAA 
CGAGAGATGA GAATAGGGAG ATTGCAGCGC CAGAAAGTCC GTGTATCCCG AAATAGGATA 
TTAGATTCTG CTGCAAAAGT TATGGAGATG TATTCTAGCC AGAAAGCTGT GCTTGAAGTA 
GAATATTTTG GTGAAGTTGG TACTGGTCTA GGCCCTACCC TTGAGTTTTA CACACTTCTA 
AGCCATGATC TGCAAAAGGC TTCCCTAGGG ATGTGGAGAT CAAGTTCTGG TGACAAGGTA 
TCTATGCAAA TTGGTAGAGA TGAGATTGAA GACGGAAAAC CATCTGCAGC TAACAGAGAT 
ATAGTTCTGG CACCACTTGG ATTGTTTCCT CGGCCTTGGC CCTCAACAGC TGACATATCT 
GAAGGTGGTC AGTTTCATAA AGTCATTGAA TATTTCCGCC TTTTAGGGCG TGTGATGGCC 
AAAGCACTTC AAGATGGACG GCTATTGGAC GTCCCATTGA GTACAGCGTT TTATAAACTT 
ATTCTTGGTC AAGAGCTTGA TTTGCATGAT ATTGTATTAT TTGACGCTGA ACTTGGCAAG 
ACCTTGCAAG AGCTGCGTGT TGTTGTTGCC CGCAAGCACT ATCTGGAGGG AGTAGGTGGT 
GACAATAGCA GCACGATTTC TGATTTATGT TTACGTGGAT GCCGAATAGA AGATCTCTCC 
TTGGAATTCA CGCTACCTGG CTATCCTGAG TACATCCTGA GATCAGGAGA TGAAATTGTT 
GATATTACTA ATCTTGAGGA GTATATATCC CTTGTCGTTG ATGCTACTGT CAAGAGAGGA 
GTCACTCGGC AGATCGAAGC CTTCAGATCT GGATTCAATC AGGTGTTTGA CATAACATCT 
CTACAAATAT TCACCCCTTC TGAGCTGGAC TATTTGCTGT GTGGTCGTAG AGAGTTGTGG 
GAGGTGGAGA CTCTTGCTGA ACATATCAAA TTTGATCATG GGTATAATGC CAAAAGTCCG 
GCAATCATTA ACTTACTGGA GATCATGGGA GAACTTACAG CAGATCAGCA GAGGGCTTTC 
TGCCAATTTG TAACTGGAGC TCCTAGGCTT CCTCCTGGTG GCTTAGCTGT TCTGAACCCA 
AAGCTTACGA TTGTGAGAAA GCACTCATCG ACCTCAAGTG CAGCAGCCAA CGGAGCAGGG 
GCTTCGGAGA CAGCAGATGA TGATTTGCCC AGTGTCATGA CTTGCGCAAA CTACCTTAAA 
CTCCCTCCTT ATTCTACAAA GGAAATCATG TACAAGAAAC TGCTCTACGC CATCAACGAA 
GGGCAAGGAT CGTTCGACCT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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KAK cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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